Vinegar powder and application thereof

Abstract

The invention relates to the field of biology, in particular to a novel preparation process and novel application of vinegar powder. An additive containing water-soluble beta-glucan, maltodextrin and beta-cyclodextrin is screened out, then a vinegar powder process is invented, firstly, sorghum is smashed, steamed and fermented, inoculated with bacteria and subjected to acetic acid fermentation, mature vinegar fermented grains are sprayed and smoked to obtain aged vinegar, the aged vinegar is blended, sterilized and filtered, food additives are added, uniform mixing is performed, and the vinegar powder is prepared. A mixed solution is obtained; and performing vacuum concentration treatment and spray drying to obtain the vinegar powder. The additive with high safety is adopted, so that the content of ligustrazine and aminobutyric acid in the vinegar powder product is higher, and the vinegar powder product has an auxiliary protection effect on ionizing radiation hazards.

Description

Technical Field

[0001] The invention relates to food additives, and based on the food additives, also relates to a new process for preparing vinegar powder and a new application thereof. Background Art

[0002] Vinegar has been used as a folk medicine since ancient times. According to the Compendium of Materia Medica, vinegar can eliminate carbuncle, disperse water vapor, kill evil toxins, and regulate various medicines. The main active ingredients in vinegar include protein, polysaccharide, ligustrazine, aminobutyric acid, polyphenols, etc. Among them, ligustrazine and aminobutyric acid have good antioxidant effects.

[0003] The vinegar powder made by spray-drying vinegar has the functions of enriching active ingredients, facilitating transportation, reducing the original irritating strong organic acid in vinegar and retaining nutrients such as ligustrazine and minerals.

[0004] Ionizing radiation is widely used in industry, medicine and military fields. However, long-term exposure to ionizing radiation can have adverse effects on human health. Radiation can cause direct and indirect damage to normal tissues and systems of the human body, especially the hematopoietic system and immune system.

[0005] At present, radiation protectors are mainly divided into two categories: one is chemical radiation protectors based on synthetic drugs, and the other is natural radiation protectors based on natural products. Traditional radiation protectors are generally chemically synthesized drugs, mainly sulfur compounds, hormone compounds, metal chelators, cytokines and other substances, which have great toxic side effects and are not suitable for long-term use. Natural products are easy to recycle and have low prices, so it is necessary to screen radiation protectors with low toxic side effects and good antioxidant capacity from natural products.

[0006] In view of this, the present invention is proposed. Summary of the invention

[0007] The invention aims to provide a functional vinegar product, for which a food additive compound is developed, and the efficacy is further improved by adding a suitable proportion of food additives with high safety and good enrichment effect. The present invention combines vacuum concentration, spray drying and other process operations, and the content of active ingredients ligustrazine and aminobutyric acid in the prepared vinegar powder is greatly improved compared with the vinegar powder using other food additive proportions. The vinegar powder prepared by the formula process has more significant antioxidant and anti-radiation effects.

[0008] In order to achieve the purpose of the invention, the technical solutions adopted are as follows:

[0009] The first aspect: a compound additive comprising water-soluble β-glucan, maltodextrin and β-cyclodextrin. The compound may comprise other components or even functional components that do not affect the activity, and is preferably composed of these three raw materials.

[0010] The mass ratio of water-soluble β-glucan, maltodextrin and β-cyclodextrin can be 0.5-2:3-6:1-4, preferably 2:5:3.

[0011] In a second aspect, the additive is added to aged vinegar to prepare a product, such as for enriching ligustrazine and / or aminobutyric acid in vinegar. The product can be a dry powder, such as vinegar powder, and its preparation includes the following steps: (2) premixing the additive with aged vinegar liquid to obtain a premixed liquid, and optionally filtering the aged vinegar before premixing to obtain a filtrate with a molecular weight of less than 4000Da; (3) spray drying to convert the premixed liquid into vinegar powder, and optionally vacuum concentrating the premixed liquid before spray drying. The aged vinegar liquid can be derived from sorghum. As a more specific example, the present invention discloses a method for preparing vinegar powder, comprising the following steps:

[0012] (1) After pulverizing sorghum, water is added to steam the material, alcohol fermentation is carried out by using Daqu, acetic acid fermentation is carried out by inoculation, and the mature vinegar mash is poured with vinegar and smoked to obtain aged vinegar liquid;

[0013] (2) blending and sterilizing the aged vinegar solution to obtain a filtrate having a molecular weight of less than 4000 Da; mixing the additive (consisting of water-soluble β-glucan, maltodextrin, and β-cyclodextrin) with the filtrate to obtain a premix;

[0014] (3) The premixed liquid is spray-dried to obtain vinegar powder.

[0015] Optionally, the acidity of the aged vinegar solution may be 4-5 g / 100 ml, the content of ligustrazine may be 10-15 mg / ml, and the content of aminobutyric acid may be 0.05-0.1 mg / ml.

[0016] Furthermore, the additive in step (2) is composed of the following components in parts by weight: 0.5-2 parts of water-soluble β-glucan, 3-6 parts of maltodextrin, and 1-4 parts of β-cyclodextrin.

[0017] Further, in step (3), the spray drying is carried out under any of the following conditions: the feed temperature is 65-70°C, the air volume can be 0.7-1.0m 3 / min, the inlet air temperature can be 120-130℃, the outlet air temperature can be 65-75℃, and the drying time can be 10-25s.

[0018] In a third aspect, the present invention discloses an aged vinegar product, such as vinegar powder, whose acidity may be 4-5g / 100g, the content of ligustrazine may be 20-40mg / g, and the content of aminobutyric acid may be 0.2-0.5mg / g.

[0019] In a fourth aspect, the present invention discloses the use of the above-mentioned vinegar product for radiation resistance, such as a new application of vinegar powder, wherein the application is to prevent radiation damage.

[0020] Optionally, the radiation damage includes radiation damage to cancer patients and radiation damage to normal people; preferably, the radiation damage to cancer patients is radiation damage during radiation treatment of cancer patients; preferably, the radiation damage to normal people is radiation damage to personnel engaged in radiation work.

[0021] Optionally, the radiation damage includes damage to the hematopoietic system and the immune system.

[0022] Optionally, the applications include: application in the preparation of a product for increasing the level of white blood cells after ionizing radiation, application in the preparation of a product for increasing the level of bone marrow DNA after ionizing radiation, and application in the preparation of a product for increasing the total antioxidant capacity (T-AOC) in serum after ionizing radiation.

[0023] Beneficial effects: The present invention uses additives to enrich the content of active ingredients, so that the obtained vinegar powder has a higher content of antioxidant active substances and better overall reducing power, and the vinegar powder can be used to improve radiation-induced damage in mice. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is the total reducing power of different vinegar powders;

[0025] Figure 2 The effect of different vinegar powders on the white blood cell count of mice after ionizing radiation;

[0026] Figure 3 The effect of different vinegar powders on the DNA content in the bone marrow of mice after ionizing radiation;

[0027] Figure 4 The effect of different vinegar powders on serum T-AOC in mice after ionizing radiation;

[0028] In the figure, the vinegar powder group is marked with ELVP and numbered according to the example number, such as ELVP-1 is the vinegar powder obtained in Example 1. DETAILED DESCRIPTION

[0029] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0030] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form also includes the plural form. In addition, it should be understood that when the terms "include" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0031] The technical solution of the present invention will be described clearly and completely in conjunction with the embodiments below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments in the invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The present invention proposes a new process for preparing vinegar powder and a new application, which can effectively enrich the antioxidant active ingredients ligustrazine and aminobutyric acid, and the vinegar powder with the best ratio of additives can be used for radiation protection. The present invention experiment found that a 6Gy dose of 60 After Kunming mice were irradiated with Go-γ rays (dose rate 2Gy / min), vinegar powder had a significant protective effect on the hematopoietic system and immune system of the irradiated experimental animals, and could increase the number of white blood cells and bone marrow DNA content in mice, and increase the T-AOC of mouse serum.

[0033] Optionally, radiation damage includes radiation damage to cancer patients and radiation damage to normal people; preferably, radiation damage to cancer patients is radiation damage during radiation therapy for cancer patients; more preferably, radiation damage to normal people is radiation damage to personnel engaged in radiation work. Radiation damage includes damage to the hematopoietic system and the immune system.

[0035] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is further described in detail below in conjunction with specific embodiments.

[0036] Note: In the following examples, the content of ligustrazine was detected by GC-MS, and the content of aminobutyric acid was detected according to QB / T4587-2013.

[0037] Example 1

[0038] (1) After pulverizing the sorghum, adding drinking water to steam the material, performing alcohol fermentation through Daqu and inoculating acetic acid fermentation, pouring vinegar on the mature fermented grains and smoking the fermented grains to obtain aged vinegar (with an acidity of 5 g / 100 ml, a ligustrazine content of 15 mg / ml, and a gamma-aminobutyric acid content of 0.1 mg / ml), and then discharging the material for later use;

[0039] (2) blending and sterilizing the aged vinegar to obtain a filtrate having a molecular weight of less than 4000 Da; mixing the additive (composition: water-soluble β-glucan: β-cyclodextrin mass ratio = 2:5) with the filtrate to obtain a mixed liquid;

[0040] (3) After vacuum concentration, the resulting mixture is spray dried to obtain vinegar powder. The spray drying conditions are feed temperature 65°C, air volume 0.7m 3 / min, inlet air temperature 120℃, outlet air temperature 70℃, drying time 20s.

[0041] The test results show that the acidity of vinegar powder is 5g / 100g, the content of ligustrazine is 17mg / g, and the content of aminobutyric acid is 0.2mg / g.

[0042] Example 2

[0043] (1) Obtain aged vinegar (acidity of 5 g / 100 ml, ligustrazine content of 15 mg / ml, and aminobutyric acid content of 0.1 mg / ml) in the manner of Example 1

[0044] (2) blending and sterilizing the aged vinegar to obtain a filtrate having a molecular weight of less than 4000 Da; mixing the additive (composed of maltodextrin and β-cyclodextrin in a mass ratio of 5:3) with the filtrate to obtain a mixed liquid;

[0045] (3) The vinegar powder was obtained by spray drying. The spray drying conditions were as follows: feed temperature 70°C, air volume 1m 3 / min, inlet air temperature 130℃, outlet air temperature 65℃, drying time 10s.

[0046] The test results show that the acidity of vinegar powder is 4g / 100g, the content of ligustrazine is 22mg / g, and the content of aminobutyric acid is 0.3mg / g.

[0047] Example 3

[0048] (1) Obtain aged vinegar (acidity of 5 g / 100 ml, ligustrazine content of 15 mg / ml, and aminobutyric acid content of 0.1 mg / ml) in the manner of Example 1

[0049] (2) blending and sterilizing the aged vinegar to obtain a filtrate having a molecular weight of less than 4000 Da; mixing an additive (comprising water-soluble β-glucan: maltodextrin: β-cyclodextrin in a weight ratio of 2:5:3) with the filtrate to obtain a mixed liquid;

[0050] (3) The mixed liquid is vacuum concentrated and spray dried to obtain vinegar powder. The spray drying conditions are feed temperature 65°C, air volume 0.9m3 / min, inlet air temperature 125℃, outlet air temperature 75℃, drying time 25s.

[0051] The test results show that the acidity of vinegar powder is 4g / 100g, the content of ligustrazine is 40mg / g, and the content of aminobutyric acid is 0.5mg / g.

[0052] Experimental Example 1 Total reducing power of different vinegar powders

[0053] Take 2 ml of three kinds of vinegar powder sample solutions of different concentrations (0.005-0.025 mg / mL) respectively, mix with 2 mL of phosphate buffer (pH 6.6, 0.2 mol / L) and 2 mL of 1% potassium ferricyanide solution, let it stand at 50°C for 20 min, add 2 mL of 10% trifluoroacetic acid to terminate the reaction, take 2 mL of supernatant and mix with 2 mL of ultrapure water and 0.4 mL of 0.1% FeCl3 solution, let it stand at 50°C for 10 min, and detect the absorbance of the mixed solution at 734 nm.

[0054] Total reducing power is an important indicator for detecting the antioxidant activity of a substance and can be used to measure its potential antioxidant activity. Figure 1 It can be seen that the vinegar powder ELVP-3 of Example 3 has a stronger total reducing power than the other two vinegar powders.

[0055] Experimental Example 2 Effects of different vinegar powders on the body weight of mice

[0056] 1. Drugs and Reagents

[0057] Test drug: vinegar powder;

[0058] Positive medicine: Licojun tablets.

[0059] 2. Experimental animals: Male Kunming mice, 6-8 weeks old, weighing 18-25 g, were raised in an animal-grade SPF barrier system and purchased from the Henan Provincial Experimental Animal Center. They were placed in dry and clean plastic cages and fed with standard experimental animal feed, with free access to water and food.

[0060] 3. Experimental method: After the mice were adaptively fed for one week, they were randomly divided into a normal control group (NC), an irradiation model group (IR), a positive control group (PC), the vinegar powder group of Example 1 (ELVP-1), the vinegar powder group of Example 2 (ELVP-2), and the vinegar powder group of Example 3 (ELVP-3), with 12 mice in each group.

[0061] Blank control group (NC): 10ml / kg bw normal saline

[0062] Irradiated control group (IR): 10ml / kg bw normal saline

[0063] Positive drug control group (PC): 12ml / kg bw Licojun tablets

[0064] Example 1 Vinegar powder group (ELVP-1): 2.7 g / kg bw

[0065] Example 2 Vinegar powder group (ELVP-2): 2.7 g / kg bw

[0066] Example 3 Vinegar powder group (ELVP-3): 2.7 g / kg bw

[0067] Each group of mice was gavaged for 30 consecutive days, and the weight changes of the mice were recorded. The test results showed that with the increase in the number of gavage days, the weight of the mice in each group continued to increase steadily, and the average daily weight of the mice gavaged with ELVP was not significantly different from that of the NC group (P>0.05), and the weight of the mice in each group was maintained at about 36g in the late stage of gavage, indicating that the mice were in a normal growth state, indicating that the highest dose of ELVP had no acute toxic side effects on mice.

[0068] Experimental Example 3 Effects of different vinegar powders on the number of white blood cells in mice after ionizing radiation

[0069] Animal experiments were performed in accordance with the method of Experimental Example 2. After continuous intragastric administration for 30 days, mice in all groups except the NC group were given 60 Co-γ ray was used for one-time uniform whole-body irradiation treatment, with a dose rate of 2Gγ / min and a total irradiation dose of 6Gγ. 24 hours after irradiation, 30μl of blood was collected from the mouse eyeball, 5μl of anticoagulant (EDTA) was added, and the blood was diluted to 240μl with buffer, and the blood was tested using a blood cell analyzer.

[0070] The experimental results are as follows Figure 2 As shown in the figure, the number of white blood cells in mice after radiation treatment changed significantly, compared with the NC group (7.84±0.62×10 9 cells / l) after irradiation. 9 cells / l) decreased sharply (P<0.001), indicating that radiation caused great damage to the body's white blood cells, leading to weakened hematopoietic function and suppression of the body's immune function. The ELVP-3 group could restore the number of white blood cells to 2.36±0.62ⅹ10 9 cells / l(P<0.05). The vinegar powder of ELVP-3 can significantly alleviate the effect of irradiation on leukocytes and has a good radiation protection effect.

[0071] Experimental Example 4 Effects of different vinegar powders on the DNA content of bone marrow in mice after ionizing radiation

[0072] Animal experiments were performed in the same manner as in Experimental Example 2. After continuous intragastric administration for 30 days, 60Co-γ ray was used for uniform whole-body irradiation at one time, with a dose rate of 2Gγ / min and a total irradiation dose of 6Gγ. On the second day after irradiation, the left intact femur was taken, and the bone marrow cells were washed down with 5mmol / l CaCl2 solution and the volume was fixed to 5ml. The obtained solution was centrifuged for 10min (2500rpm), the supernatant was discarded, and the precipitate was retained. Then 5ml of 0.20mol / l HClO4 was added, mixed and reacted at 90℃ for 15min, cooled, and centrifuged. The supernatant was taken and the absorbance value was measured at a wavelength of 260nm.

[0073] The experimental results are as follows Figure 3 As shown in the results, after irradiation, the bone marrow DNA content in the IR group (0.244±0.066mg, P<0.001) showed a very significant decrease compared with the NC group (1.029±0.070mg). Compared with the IR group, the bone marrow DNA content of ELVP-3 mice increased significantly to 0.304±0.062mg (P<0.05). ELVP-3 can significantly increase the bone marrow DNA content of mice after irradiation, alleviate the damage of radiation to the bone marrow of mice, and thus play a role in protecting the hematopoietic system.

[0074] Experimental Example 5 Effects of different vinegar powders on serum T-AOC content in mice after ionizing radiation

[0075] Animal experiments were performed in accordance with the method of Experimental Example 2. After continuous intragastric administration for 30 days, mice in all groups except the NC group were given 60 The mice were irradiated uniformly with Co-γ rays at one time, with a dose rate of 2Gγ / min and a total irradiation dose of 6Gγ. 24 hours after irradiation, blood was collected from the eyeballs of the mice, and serum was collected by static centrifugation, and the T-AOC content was detected according to the kit.

[0076] Depend on Figure 4 As shown, the T-AOC content in the serum of mice decreased significantly after ionizing radiation. The vinegar powder of ELVP-3 can significantly increase the level of T-AOC content in the serum of mice after ionizing radiation, indicating that the vinegar powder of Example 3 has significant antioxidant and anti-radiation effects in vivo.

[0077] Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the claims. Any technical personnel in this field may make several possible changes and modifications without departing from the concept of the present application. Therefore, the scope of protection of the present application shall be based on the scope defined by the claims of the present application.

Claims

1. An additive for preparing dry powder from aged vinegar liquid, comprising water-soluble β-glucan, maltodextrin and β-cyclodextrin (preferably consisting of them).

2. The additive according to claim 1, characterized in that: The mass ratio of the water-soluble β-glucan, maltodextrin and β-cyclodextrin is 0.5-2:3-6:1-4, preferably 2:5:

3.

3. A method for preparing vinegar powder using the additive of any preceding claim, comprising the following steps: (2) premixing the additive with aged vinegar to obtain a premixed solution, and optionally filtering the aged vinegar before premixing to obtain a filtrate having a molecular weight of less than 4000 Da; (3) Spray drying to convert the premixed liquid into vinegar powder, and optionally concentrating the premixed liquid before spray drying.

4. The method according to the preceding claim, characterized in that The spray drying conditions are feed temperature 65-70 °C, inlet air temperature 120-130 °C, outlet air temperature 65-75 °C; the air volume can be 0.7-0.9 m 3 / min, optionally, the drying time is 10-25 s.

5. A method as claimed in any preceding claim, characterised in that The content of ligustrazine in the aged vinegar liquid is 10-15 mg / ml, the content of aminobutyric acid is 0.05-0.1 mg / ml, and the acidity is optionally 4-5 g / 100g.

6. A method as claimed in any preceding claim, characterised in that The aged vinegar liquid is derived from sorghum, and its preparation may include: After the sorghum is crushed, water is added to steam it. After alcohol fermentation through Daqu, acetic acid fermentation is inoculated, and the mature vinegar mash is poured with vinegar and smoked to obtain aged vinegar.

7. Vinegar powder obtainable by the process of any preceding claim.

8. Use of the vinegar powder according to the preceding claim in the preparation of products for resisting and / or preventing radiation damage.

9. Use according to the preceding claim, characterized in that The radiation damage includes damage to the hematopoietic system and / or the immune system.

10. The use according to any preceding claim, characterized in that The product can produce at least one of the following effects: improving the white blood cell level after ionizing radiation, improving the bone marrow DNA level after ionizing radiation, and improving the total antioxidant capacity T-AOC in serum after ionizing radiation.

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