Stevia rebaudianum culture process

Abstract

The invention provides a stevia rebaudianum culture process, and relates to the technical field of stevia rebaudianum culture, and the stevia rebaudianum culture process comprises the following steps: 1, environment disinfection; step 2, strain extraction; 3, preparing a culture medium; step 4, inoculation and culture; 5, extracting and purifying a product; and step 6, quality detection and analysis. The method has the advantages that sterilization and disinfection are carried out before strain extraction, then a special culture environment is set for stevia rebaudiana, in this way, the inhibition effect on other strains can be achieved, finally, physical screening is carried out for formed strain communities, and therefore the purity of the strains is guaranteed to the maximum extent.

Description

Technical Field

[0001] The present invention relates to the technical field of stevia cultivation, and in particular to a stevia cultivation process. Background Art

[0002] Most of the existing stevia bacteria are directly cultured using parts of the stevia plant, which can easily lead to the contamination of other bacteria on the stevia plant with the stevia bacteria, thus greatly affecting the purity of the stevia strains, causing great trouble for subsequent extraction and not conducive to ensuring the concentration of stevia bacteria. Summary of the invention

[0003] The purpose of the present invention is to solve the shortcomings of the prior art and propose a stevia culture process. The technical solution adopted by the present invention is:

[0004] A stevia culture process comprises the following steps:

[0005] Step 1: Environmental disinfection;

[0006] Step 2: Extracting bacteria;

[0007] Step 3: Preparation of culture medium;

[0008] Step 4: Inoculation and cultivation;

[0009] Step 5: product extraction and purification;

[0010] Step 6: Quality inspection and analysis.

[0011] As an improvement, the operation method of step 1, environmental disinfection is:

[0012] The stevia plant is sterilized by pasteurization, and the extraction environment is also sterilized. The sterilization temperature should be 60-82°C and maintained for thirty minutes.

[0013] As an improvement, the operation method of step 2, strain extraction is:

[0014] The parts of the stevia plant are separated after disinfection, and then the separated parts are soaked. After soaking for a certain period of time, the soaked liquid is extracted, and then the extracted liquid is concentrated. The concentrated liquid is then placed in the corresponding culture dish for culture. During the culture process, the colonies must be tested to find the species of stevia.

[0015] As an improvement, the operation method of step 3, preparing the culture medium is as follows:

[0016] The corresponding culture medium is configured according to the extracted bacterial species and the environment of the culture site, so that the environment inside the culture medium is conducive to the growth of the Stevia species.

[0017] As an improvement, the operation steps of step 4, inoculation and cultivation are as follows:

[0018] Then, the strain obtained in step 2 is inoculated into the culture medium prepared in step 3, so that the strain of stevia can grow and ferment normally. After fermentation for six months, stevia and other residual fungi are obtained to form a variety of biological bacteria.

[0019] As an improvement, the operating steps of step 5, product extraction and purification are as follows:

[0020] According to step 4, a plurality of biological bacteria are formed, and then stevia is extracted from the plurality of biological bacteria. The stevia can be screened by filtering and sterilizing, so that bacteria larger or smaller than stevia are removed, thereby achieving the extraction and purification of stevia.

[0021] As an improvement, the operation steps of step 6, quality inspection and analysis are as follows:

[0022] The extracted and purified stevia is subjected to quality inspection and analysis to determine whether the required stevia purity and other requirements are met.

[0023] The beneficial effects of the present invention are:

[0024] The stevia culture process of the present invention first performs sterilization and disinfection before strain extraction, then sets a special culture environment for stevia, which can inhibit other strains, and finally performs physical screening on the formed strain community, thereby ensuring the purity of the strain to the greatest extent possible. DETAILED DESCRIPTION

[0026] A stevia culture process comprises the following steps:

[0027] Step 1: Environmental disinfection:

[0028] Use pasteurization to sterilize the stevia plant and the extraction environment. The sterilization temperature should be 60-82℃ for 30 minutes.

[0029] Step 2: Extraction of bacteria:

[0030] Separate the parts of the stevia plant after disinfection, and then soak the separated parts. After soaking for a certain period of time, extract the soaked liquid, and then concentrate the extracted liquid. Then put the concentrated liquid into the corresponding culture dish for culture. During the culture process, the colony should be detected to find the strain of stevia.

[0031] Step 3: Preparation of culture medium:

[0032] The corresponding culture medium is configured according to the extracted bacterial species and the environment of the culture site, so that the environment inside the culture medium is conducive to the growth of the stevia species;

[0033] Step 4: Inoculation and cultivation:

[0034] Then, the strain obtained in step 2 is inoculated into the culture medium prepared in step 3, so that the strain of stevia can grow and ferment normally. After fermentation for six months, stevia and other residual fungi are obtained to form a variety of biological bacteria;

[0035] Step 5: Product extraction and purification:

[0036] According to step 4, a plurality of biological bacteria are formed, and then stevia is extracted from the plurality of biological bacteria. The stevia can be screened by filtering and sterilizing, so that bacteria larger or smaller than stevia are removed, thereby achieving extraction and purification of stevia;

[0037] Step 6: Quality inspection and analysis:

[0038] The extracted and purified stevia is subjected to quality inspection and analysis to determine whether the required stevia purity and other requirements are met.

[0039] Before the strains are extracted, sterilization and disinfection are first carried out, and then a special culture environment is set up for Stevia, which can have an inhibitory effect on other strains. Finally, the formed bacterial communities are physically screened to ensure the purity of the strains to the greatest extent possible.

[0040] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A stevia culture process, characterized in that: The following steps are involved: Step 1: Environmental disinfection; Step 2: Extracting bacteria; Step 3: preparing culture medium; Step 4: Inoculation and cultivation; Step 5: product extraction and purification; Step 6: Quality inspection and analysis.

2. A stevia culture process according to claim 1, characterized in that: The operation method of step 1, environmental disinfection is: The stevia plant is sterilized by pasteurization, and the extraction environment is also sterilized. The sterilization temperature should be 60-82°C and maintained for thirty minutes.

3. A stevia culture process according to claim 1, characterized in that: The operation method of step 2, strain extraction is as follows: The parts of the stevia plant are separated after disinfection, and then the separated parts are soaked. After soaking for a certain period of time, the soaked liquid is extracted, and then the extracted liquid is concentrated. The concentrated liquid is then placed in the corresponding culture dish for culture. During the culture process, the colonies must be tested to find the species of stevia.

4. A stevia culture process according to claim 1, characterized in that: The operation method of step 3, preparing the culture medium is as follows: The corresponding culture medium is configured according to the extracted bacterial species and the environment of the culture site, so that the environment inside the culture medium is conducive to the growth of the Stevia species.

5. A stevia culture process according to claim 1, characterized in that: The operation steps of step 4, inoculation and cultivation are as follows: Then, the strain obtained in step 2 is inoculated into the culture medium prepared in step 3, so that the strain of stevia can grow and ferment normally. After fermentation for six months, stevia and other residual fungi are obtained to form a variety of biological bacteria.

6. A stevia culture process according to claim 1, characterized in that: The operation steps of step 5, product extraction and purification are as follows: According to step 4, a plurality of biological bacteria are formed, and then stevia is extracted from the plurality of biological bacteria. The stevia can be screened by filtering and sterilizing, so that bacteria larger or smaller than stevia are removed, thereby achieving the extraction and purification of stevia.

7. A stevia culture process according to claim 1, characterized in that: The operation steps of step 6, quality inspection and analysis are as follows: The extracted and purified stevia is subjected to quality inspection and analysis to determine whether the required stevia purity and other requirements are met.