Application of agrobacterium tumefaciens C53 in promoting soil nitrification and plant growth
By screening and isolating Agrobacterium tumefaciens C5_3 and inoculating the strain in the soil, the problems of soil nitration and low plant growth efficiency in the prior art were solved, significant acceleration of soil nitration and the promotion of corn growth and development were achieved, and a new agricultural microbial resource was provided.
Patent Information
- Application Number
- CN202411971193.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-04-25
AI Technical Summary
No research and report on Agrobacterium tumefaciens in promoting plant growth and promoting soil nitration has been seen in the prior art, resulting in low nitrogen conversion efficiency and limited plant growth in agricultural production.
Agrobacterium tumefaciens strain C5_3 (classified as Agrobacterium tumefaciens strain C5_3) was screened and isolated, and the strain was inoculated in the soil to promote soil nitration and plant growth and development.
Agrobacterium tumefaciens C5_3 significantly promotes soil nitration and corn growth and development, improves crop yield and quality, and provides a new microbial resource suitable for promotion and use in corn cultivation areas across the country.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of microorganisms and their applications, and relates to Agrobacterium tumefaciens bacteria for promoting soil nitrification and plant growth and development and applications thereof. Background Art
[0002] Soil nitrification is one of the important nitrogen transformation processes in the soil, which can convert ammonium nitrogen into nitrate nitrogen, and the produced nitrate nitrogen is an important source of soil nitrogen. Nitrogen, as an essential macronutrient for plants, is the main factor affecting plant growth and yield. Therefore, regulating soil nitrification by appropriate means to promote crop growth and development is of great significance to agricultural production.
[0003] Agrobacterium tumefaciens is widely present in natural environments such as soil and river sediments. Currently, there are many reports on the research of Agrobacterium tumefaciens in the field of environmental protection, but there are no research reports on its promotion of plant growth and soil nitrification in the prior art.
[0004] Invention Technology The technical objectives of the present invention are: 1. To provide the application of Agrobacterium tumefaciens C5_3 in promoting soil nitrification; 2. To provide a method for promoting soil nitrification; 3. To provide the application of Agrobacterium tumefaciens C5_3 in promoting plant growth and development; 4. To provide a method for promoting plant growth and development; The present invention explores the function of Agrobacterium tumefaciens C5_3 screened from the soil and finds that it has the effects of promoting plant growth and development and promoting soil nitrification, thereby providing a new technical path for agricultural production.
[0005] In order to achieve the above object, the specific scheme adopted by the present invention is: application of Agrobacterium tumefaciens C5_3 in promoting soil nitrification, wherein the Agrobacterium tumefaciens C5_3 is classified as Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
[0006] A method for promoting soil nitrification, inoculating Agrobacterium tumefaciens C5_3 in the soil; the Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
[0007] Application of Agrobacterium tumefaciens C5_3 in promoting plant growth and development, wherein the Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strainC5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
[0008] Furthermore, the plant is corn.
[0009] A method for promoting plant growth and development, inoculating Agrobacterium tumefaciens C5_3 in soil; the Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
[0010] Furthermore, before sowing the plants, the plant seeds are evenly mixed with Agrobacterium tumefaciens C5_3 and then planted in the soil.
[0011] Beneficial effects: The present invention aims to promote plant growth, screens and separates Agrobacterium tumefaciens C5_3 from the soil, and identifies and analyzes the growth and development of plants before and after seed dressing and the promotion of soil nitrification, and finds that Agrobacterium tumefaciens C5_3 has significant effects in promoting plant growth and development and promoting soil nitrification, and can be applied to crop growth, providing a new microbial resource for agricultural production. In addition, the microbial agent prepared by using the Agrobacterium tumefaciens C5_3 has the advantages of low production cost and convenient use, and is suitable for promotion and use in corn-growing areas across the country.
[0012] Bacterium deposit information: The Agrobacterium tumefaciens C5_3 described in the present invention is classified and named Agrobacterium tumefaciens strain C5_3 has been deposited in the China Center for Type Culture Collection. The deposit date is November 5, 2024. The deposit address is Wuhan University, Wuhan, China. The deposit number is CCTCC NO: M 20242432. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 This is a colony morphology diagram of Agrobacterium tumefaciens C5_3 in a separation medium that has the function of promoting soil nitrification and promoting plant growth and development according to the present invention; Figure 2 This is an optical microscope image of the colony morphology of Agrobacterium tumefaciens C5_3 that has the function of promoting soil nitrification and promoting plant growth and development according to the present invention. DETAILED DESCRIPTION
[0014] The technical solution of the present invention is described more clearly and completely below in conjunction with the accompanying drawings and embodiments.
[0015] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.
[0016] The inoculation amounts described in the following examples are all inoculated according to the corresponding volume ratios.
[0017] Example 1: Isolation and screening of Agrobacterium tumefaciens C5_3 that promotes soil nitrification and plant growth and development Take 20g of soil from the experimental farm of Henan University of Science and Technology and add it to the enrichment medium, the formula of which is: (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.75 g, NaH 2 PO 4 0.25 g, MnSO 4 ∙4H 2 O 0.01 g, MgSO 4 ∙7H 2 O 0.03 g, CaCO 3 5.0g, deionized water is added to 1000ml, pH value is 7.0-7.2, sterilized at 121℃ for 20min, and cultured at 28℃ in the dark for 15-25 days. Then, Griess reagent is used to detect the presence of ammonia oxidizing bacterial strains, and the enriched culture solution showing the growth of ammonia oxidizing bacterial strains is transferred to a new enriched culture medium for further culture. This process is repeated twice to obtain a pure bacterial enriched solution.
[0018] Take 0.1 ml of the enriched culture solution obtained above, spread it on the agar plate separation medium, and culture it at 28°C and in the dark for 7 days. The formula of the agar plate separation medium is: (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 0.75 g, NaH 2 PO 4 0.25 g, MnSO 4 ∙4H 2 O 0.01g, MgSO 4 ∙7H 2 O 0.03 g, CaCO 35.0 g, 2.5 g agar, deionized water to 1000 ml, pH 7.0-7.2, sterilized at 121°C for 20 minutes. After 7 days, single colonies were selected from the above separation medium and spread on the separation medium of the agar plate. This process was repeated 5-10 times to obtain a plate medium containing pure bacterial colonies.
[0019] On the plate culture medium containing pure bacterial colonies obtained above, 5 to 10 pure colonies were transplanted into an enrichment culture medium (formula as above), and cultured at 28°C in the dark for 15 days to obtain the Agrobacterium tumefaciens C5_3 microbial agent described in this study.
[0020] The above Agrobacterium tumefaciens C5_3, which has the function of promoting soil nitrification and promoting plant growth and development, is named C5_3, and its classification is named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number being CCTCC NO: M 20242432.
[0021] Example 2: Determination of the effects of Agrobacterium tumefaciens C5_3 on promoting soil nitrification and plant growth and development Take 12 pots, each pot is designed to be planted with 3 corns, and the pots are filled with soil. It is determined that each kilogram of soil in the pot contains 25.7g of organic matter. The 12 pots of corn are randomly divided into 3 groups, each with 4 pots, namely test groups a, b, and c, and the 4 pots in each group represent 4 repetitions.
[0022] 200 ml of bacterial solution was added to 1 kg of corn seeds for seed dressing. Experimental group a was used as a blank control, and corn seeds were directly sown. In experimental group b, corn seeds were mixed with bacterial solution in each pot when sowing. In experimental group c, the same proportion of pure enrichment solution was added to the seeds in each pot when sowing, stirred, and then sown as a control; that is, experimental group b was corn seeds mixed with Agrobacterium tumefaciens C5_3 bacterial agent, and experimental group c was corn seeds mixed with enrichment solution.
[0023] Take out the roots, stems and leaves of corn seedlings grown 20 days after sowing, test group a; test group b; and test group c. Put these roots, stems and leaves into a drying box at a temperature of 75°C, and after drying, obtain the whole corn plant dried products, and then use an electronic balance to detect the weight of the dried products to obtain the dried product mass Ra of test group a, the dried product mass Rb of test group b, and the dried product mass Rc of test group c, and set them aside.
[0024] The soil nitrification rate of the rhizosphere soil of a, b, and c was determined by the culture method, and the soil nitrification rate Za after inoculation of the test group a was 0.047, the soil nitrification rate Zb after inoculation of the test group b was 0.182, and the soil nitrification rate Zc after inoculation of the test group c was 0.072, which were set aside for later use.
[0025] The test results show that Ra, Rb, and Rc are 2.21g, 3.34g, and 2.54g / pot, respectively. Comparing the sizes of Ra, Rb, and Rc, Rb>Rc>Ra, and Zb>Zc>Za, indicating that inoculation with Agrobacterium tumefaciens C5_3 can effectively promote the acceleration of soil nitrification and promote the growth and development of corn. That is, inoculation with Agrobacterium tumefaciens C5_3 can accelerate the growth and development of corn by promoting nitrification in the rhizosphere soil.
[0026] It should be noted that the above-described embodiments should be understood as illustrative rather than limiting the scope of protection of the present invention, and the scope of protection of the present invention shall be subject to the claims. For those skilled in the art, some non-essential improvements and adjustments made to the present invention still fall within the scope of protection of the present invention without departing from the essence and scope of the present invention.
Claims
1. Application of Agrobacterium tumefaciens C5_3 in promoting soil nitrification, characterized in that: The Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
2. A method for promoting soil nitrification, characterized in that: Inoculate Agrobacterium tumefaciens C5_3 in soil; the Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
3. Application of Agrobacterium tumefaciens C5_3 in promoting plant growth and development, characterized in that: The Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
4. The use of Agrobacterium tumefaciens C5_3 in promoting plant growth and development according to claim 3, characterized in that: The plant is corn.
5. A method for promoting plant growth and development, characterized in that: Inoculate Agrobacterium tumefaciens C5_3 in soil; the Agrobacterium tumefaciens C5_3 is classified and named Agrobacterium tumefaciens strain C5_3 was deposited in the China Center for Type Culture Collection on November 5, 2024, with the deposit number CCTCC NO: M 20242432.
6. A method for promoting plant growth and development according to claim 5, characterized in that: Before sowing the plants, mix the plant seeds with Agrobacterium tumefaciens C5_3 evenly and then plant them in the soil.