Application of jointly inoculated rhizosphere phosphate solubilizing bacteria in plant growth promotion and soil nutrient improvement

Abstract

The invention discloses application of jointly inoculated rhizosphere phosphate solubilizing bacteria in plant growth promotion and soil nutrient improvement, and belongs to the technical field of microorganisms. The acinetobacter and pantoea combined inoculation provided by the invention can effectively convert insoluble inorganic phosphorus into soluble inorganic phosphorus, thereby promoting the utilization of phosphorus. The ratio of acinetobacter to pantoea combined inoculation is (4-6): (6-4), the soil phosphorus dissolving capacity and the plant growth promoting effect are remarkable, and the compound fertilizer can be applied to improving the soil phosphorus utilization rate and promoting plant growth and has important agricultural and environmental values.

Description

Technical Field The present invention belongs to the field of microbial technology and relates to the application of combined inoculation of rhizosphere phosphate-solubilizing bacteria in promoting plant growth and improving soil nutrients, and specifically relates to the application of combined inoculation of Acinetobacter and Pantoea strains in soil phosphate solubilization and plant growth promotion. Background Art Phosphorus is one of the macronutrients necessary for plant growth and development. However, more than 90% of the phosphorus in the soil is ineffective, which limits the absorption and utilization of phosphorus nutrition by plants. Phosphate-solubilizing bacteria can convert the insoluble phosphorus in the soil into effective phosphates that can be absorbed by plants, improve the utilization rate of phosphorus in the soil, and promote plant growth. At present, the phosphate-solubilizing bacteria fertilizer preparations widely used in the market are mainly Bacillus, and the joint inoculation of multiple phosphate-solubilizing bacteria may not be better than a single inoculation in promoting soil and plant growth. This may be due to the antagonistic effect between multiple bacteria, and the activity is inhibited when co-inoculated, thereby reducing its promoting effect. Therefore, studying the appropriate ratio of phosphate-solubilizing bacteria will not only provide new bacterial resources for the development of bacterial fertilizers, but also has important application value. Summary of the invention

[0001] A method for promoting plant growth, wherein Pantoeacypripedii and Acinetobacter sp. are added to a plant culture system; The Pantoea strain Pantoea cypripedii was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33054; The Acinetobacter sp. was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33053.

[0002] The method according to [1], wherein the Pantoea cypripedii strain and Acinetobacter sp. are added in a ratio of (4-6):(6-4).

[0003] The method according to [2], wherein the total concentration of Pantoea cypripedii and Acinetobacter sp. is not less than 10 6 cfu / mL.

[0004] The method according to any one of [1] to [3], wherein the plant comprises tomato, wheat, rice, corn, cotton, rapeseed, soybean, pea, preferably tomato.

[0005] The method according to any one of [1] to [4], wherein the promoting plant growth includes at least one of promoting plant height, promoting stem circumference, increasing aboveground fresh weight, and increasing underground fresh weight.

[0006] A method for improving soil nutrient status, wherein Pantoeacypripedii and Acinetobacter sp. are added to the soil; The Pantoea strain Pantoea cypripedii was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33054; The Acinetobacter sp. was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33053.

[0007] The method according to [6], wherein the Pantoea cypripedii strain and Acinetobacter sp. are added in a ratio of (4-6): (6-4).

[0008] The method according to [6] or [7], wherein the total concentration of Pantoea cypripedii and Acinetobacter sp. is not less than 10 6 cfu / mL.

[0009] The method according to any one of [5] to [8], wherein the improving soil nutrient status comprises: increasing the content of available potassium in the soil, increasing the content of available phosphorus in the soil, and increasing the content of organic matter in the soil. Beneficial effects: The present invention has significant soil phosphorus solubilization ability and plant growth promotion effect by inoculating the combined Acinetobacter and Pantoea strains in a ratio of (4-6): (6-4), and can be used to improve soil phosphorus utilization and promote plant growth, and has important agricultural and environmental values. Biomaterial Deposit The phosphate-solubilizing bacteria PoPSB-1 ​​provided by the present invention is classified and named Acinetobacter sp., and has been deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on December 12, 2024, with a deposit number of CGMCC No.33053, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The phosphate-solubilizing bacteria PoPSB-2 provided by the present invention is classified and named Pantoea cypripedii, and has been deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on December 12, 2024, with a deposit number of CGMCC No. 33054, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 The growth status of tomato seedlings in Example 1 is shown. DETAILED DESCRIPTION The inventors previously screened two strains of phosphate-solubilizing bacteria from the rhizosphere soil of peony 'Fengdan', which can convert insoluble phosphorus into soluble phosphorus, namely PoPSB-1 ​​and PoPSB-2. Among them, PoPSB-1 ​​is Acinetobacter, and PoPSB-2 is a strain of Pantoea. Since tomatoes are plants that rely on soluble phosphorus for growth and development. Soluble phosphorus is a key factor in biological processes such as photosynthesis, energy metabolism and signal transduction in tomato plants. Soluble phosphorus in the soil can be absorbed by the tomato root system and converted into phosphorus compounds required by the plant, promoting the formation and ripening of the fruit. However, usually, phosphorus in the soil mostly exists in a poorly soluble form and is difficult to be used by tomatoes. Therefore, the inventors tried to add the screened phosphate-solubilizing strains PoPSB-1 ​​and PoPSB-2 to the soil for cultivating tomatoes, and combined the two in different ratios. They unexpectedly found the best ratio, which can effectively promote the growth of tomatoes and also has a significant improvement effect on soil nutrients (for example, it can promote the content of available potassium, available phosphorus, and organic matter in the soil). In this article, "available potassium" refers to potassium in the soil that is easily absorbed and utilized by crops, including soil solution potassium and soil exchangeable potassium. Available potassium accounts for 0.1% to 2% of the total potassium in the soil, of which soil solution potassium accounts for 1% to 2% of available potassium. Due to its low proportion, it is often included in exchangeable potassium. The content of available potassium is one of the important indicators to characterize the supply of soil potassium. In this article, "available phosphorus", also known as fast-acting phosphorus, is the phosphorus component in the soil that can be absorbed by plants. It includes all water-soluble phosphorus, some adsorbed phosphorus and organic phosphorus, and some soils also include some precipitated phosphorus. In this article, "organic matter" refers to substances in the soil that come from life, including soil microorganisms and soil animals and their secretions, as well as plant residues and plant secretions in the soil. Example 1 The phosphate-solubilizing strains PoPSB-1 ​​and PoPSB-2 screened in the early stage were inoculated into 40 mL LB liquid medium, respectively, and cultured overnight at 180 r / min and 30 °C. After 18 h, the bacterial solution was collected by centrifugation, and the bacteria were washed twice with 40 mL of sterile water. Finally, sterile water was added to adjust the bacterial solution concentration by 10 6 cfu / mL. Preparation of bacterial suspensions with different ratios: PoPSB-1:PoPSB-2=4:6, that is, the concentration of PoPSB-1 ​​is 4×10 5 cfu / mL, the concentration of PoPSB-2 was 6×10 5 cfu / mL; PoPSB-1:PoPSB-2=6:4, that is, the concentration of PoPSB-1 ​​is 6×10 5 cfu / mL, the concentration of PoPSB-2 was 4×10 5 cfu / mL; PoPSB-1:PoPSB-2=2:8, that is, the concentration of PoPSB-1 ​​is 2×10 5 cfu / mL, the concentration of PoPSB-2 was 8×10 5 cfu / mL; PoPSB-1:PoPSB-2=8:2, that is, the concentration of PoPSB-1 ​​is 8×10 5 cfu / mL, the concentration of PoPSB-2 was 2×10 5 cfu / mL. Each pot was filled with 1.0 kg of test soil, and 30 tomato seedlings with basically the same growth potential were selected and planted in it. 40 mL of the above-mentioned bacterial suspension with different ratios (PoPSB-1:PoPSB-2=4:6 or 6:4 or 2:8 or 8:2) was added to the treatment group, and 40 mL of sterile water was used as the control. Six replicates were set for both the treatment group and the control group. The root irrigation method was used during inoculation, and the inoculation was placed in an artificial climate incubator for culture. After 25 days, samples were taken to measure the growth index of tomato seedlings and the nutrient content of soil. Growth of tomato seedlings Figure 1 As shown, Table 1. Tomato growth indicators: Table 2. Soil nutrient content: Although the present invention has been disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for promoting plant growth, characterized in that: Adding Pantoea cypripedii and Acinetobacter sp. to the plant culture system; The Pantoea strain Pantoea cypripedii was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33054; The Acinetobacter sp. was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33053.

2. The method according to claim 1, characterized in that The Pantoea cypripedii strain and Acinetobacter sp. are added in a ratio of (4-6): (6-4).

3. The method according to claim 2, characterized in that The total concentration of the Pantoea cypripedii strain and Acinetobacter sp. is not less than 10 6 cfu / mL.

4. The method according to any one of claims 1 to 3, characterized in that: The plants include tomato, wheat, rice, corn, cotton, rapeseed, soybean, pea, preferably tomato.

5. The method according to any one of claims 1 to 4, characterized in that: The promoting of plant growth comprises at least one of promoting plant height, promoting stem circumference, increasing the fresh weight of above-ground parts and increasing the fresh weight of underground parts.

6. A method for improving soil nutrient status, characterized in that: Pantoeacypripedii and Acinetobacter sp. were added to the soil; The Pantoea strain Pantoea cypripedii was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33054; The Acinetobacter sp. was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 12, 2024, with the deposit number CGMCC No.33053.

7. The method according to claim 6, characterized in that The Pantoea cypripedii strain and Acinetobacter sp. are added in a ratio of (4-6): (6-4).

8. The method according to claim 6 or 7, characterized in that: The total concentration of the Pantoea cypripedii strain and Acinetobacter sp. is not less than 10 6 cfu / mL.

9. The method according to any one of claims 5 to 8, characterized in that: The improvement of soil nutrient conditions includes: increasing the content of available potassium in the soil, increasing the content of available phosphorus in the soil, and increasing the content of organic matter in the soil.

Images