Chronic stress experimental device for experimental small animals and experimental method thereof

Abstract

The invention discloses an experimental small animal chronic stress experimental device and an experimental method thereof, and relates to the technical field of animal chronic stress experiments.The technical scheme includes that the experimental small animal chronic stress experimental device comprises an experimental box, an illuminating lamp is arranged in the experimental box, a camera is arranged on the right side of the illuminating lamp, a buzzer is arranged on the rear side of the camera, and a smell throwing opening is formed in the top of the experimental box; the lower part of the experiment box is fixedly connected with a filter plate, the lower part of the filter plate is provided with an excrement collecting tank, the excrement collecting tank is in sliding connection with the experiment box, the upper part of the experiment box is provided with a transmission mechanism, and the lower part of the transmission mechanism is fixedly connected with a movable plate. When the push plate moves, the excrement suction heads arranged on the two sides can effectively suck excrement on the surface of the filter plate, and small animal urine remaining on the surface of the filter plate can be cleaned through the water suction cloth liner arranged at the bottom of the push plate.

Description

Technical Field

[0001] The invention relates to the technical field of animal chronic stress experiments, in particular to a small animal chronic stress experiment device and an experiment method thereof. Background Art

[0002] The background technology of the experimental device for chronic stress of experimental small animals mainly stems from the research needs of the behavior and physiological responses of experimental animals under chronic stress conditions. The design of the device aims to simulate a variety of chronic stressors, such as crowded environment, day and night reversal, noise, odor of natural enemies, etc., in order to explore the impact of these stress factors on the mental health and physiological functions of experimental animals. Traditional experimental methods are often difficult to apply these stress factors comprehensively and systematically, and the operation is cumbersome and inefficient. Therefore, an experimental device that can automatically and intelligently realize a variety of stress stimuli has been developed to improve experimental efficiency, reduce labor costs, and more accurately simulate the chronic stress state of experimental animals in the natural environment, providing strong support for related research.

[0003] After searching, the Chinese patent number CN220831384U discloses a chronic stress experimental device for experimental small animals, including an experimental box, a load-bearing partition is fixed at the lower part of the experimental box to divide the internal space of the experimental box into an upper cavity for carrying multiple experimental small animals and a lower cavity for placing a waste tray, a plurality of leakage holes are opened on the load-bearing partition, so that the waste generated by the experimental small animals can leak into the waste tray through the leakage holes, an upper box door is hinged on the front side of the experimental box and corresponds to the position of the upper cavity, a lower box door is hinged on the front side of the experimental box and corresponds to the position of the lower cavity, an adjustable box tilt component is fixed at the bottom of the experimental box, the experimental box is fixed to the experimental table through the bottom of the adjustable box tilt component, the experimental box is provided with an adjustable space crowding component, an adjustable day and night reversal component and a natural enemy odor release component, and an adjustable strobe component and a noise playing component are provided in the experimental box. Compared with the prior art, the Chinese patent number CN220831384U solves the problem that it is very troublesome to complete the chronic stress experiment on experimental animals. If the experimental animals are changed to different devices to achieve the corresponding stress stimulation, this will affect the accuracy of the final experimental results.

[0004] However, in actual use of the above device, it is difficult to deal with the feces and urine discharged by the small animals in the experimental box in time, which can easily affect the living environment of the small animals and increase the impact of non-stress factors on the experimental results. Therefore, an experimental small animal chronic stress experimental device and an experimental method are proposed. Summary of the invention

[0005] The purpose of the present invention is to solve the problem in the prior art that the feces and urine discharged by small animals in the experimental box are difficult to be processed in time, which can easily affect the living environment of the small animals and increase the influence of non-stress factors on the experimental results. A chronic stress experimental device and an experimental method for experimental small animals are proposed.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A small animal chronic stress experimental device and an experimental method thereof, comprising an experimental box, wherein an illuminating lamp is arranged inside the experimental box, a camera is arranged on the right side of the illuminating lamp, a buzzer is arranged on the rear side of the camera, an odor delivery port is arranged on the top of the experimental box, a filter plate is fixedly connected to the lower part of the experimental box, a feces collecting trough is arranged at the lower part of the filter plate, the feces collecting trough is slidably connected to the experimental box, a transmission mechanism is arranged on the upper part of the experimental box, a movable plate is fixedly connected to the lower part of the transmission mechanism, a cleaning mechanism is arranged at the front end of the filter plate, the cleaning mechanism comprises a slide fixedly connected to the inside of the experimental box, a push plate is slidably connected to the inside of the slide, a second motor is arranged on the upper part of the push plate, a first gear is arranged at the output end of the second motor, the first gear is meshedly connected to the second gear, the second gear is meshedly connected to the third gear, a transmission rack is meshedly connected to the right side of the third gear, and the transmission rack is fixedly connected to the experimental box;

[0008] A disinfectant tank is fixedly connected to the upper part of the experimental box, two disinfectant nozzles are arranged at the rear end of the disinfectant tank, an adjustment mechanism is arranged at the upper part of the disinfectant nozzle, collection boxes are fixedly connected to both sides of the disinfectant tank, a feces suction head is fixedly connected to the rear end of the collection box, an air pump is arranged inside the feces suction head, after the small animal defecates on the filter plate, the second motor is started, and the third gear is driven by the second motor to move along the meshing transmission rack, thereby driving the push plate to move along the bottom of the filter plate, and the feces of the small animal is absorbed by the feces suction head during the movement of the push plate, and after the absorption is completed, the disinfectant is sprayed by the disinfectant nozzle to disinfect the surface of the filter plate, a pressurized water pump is arranged inside the disinfectant nozzle, and a water-absorbing pad is arranged at the contact part of the bottom of the push plate with the surface of the filter plate.

[0009] The above technical solution further includes:

[0010] A water supply pipe is fixedly connected to the left side of the experimental box, a water tank is arranged at the lower part of the water supply pipe, and the water tank is fixedly connected to the experimental box.

[0011] A food pipe is fixedly connected to the left side of the experimental box, a food trough is arranged at the lower part of the food pipe, and the food trough is fixedly connected to the experimental box.

[0012] The left and right sides of the experimental box are symmetrically provided with vents, and the front end of the experimental box is provided with a box door.

[0013] The adjusting mechanism comprises an adjusting housing fixedly connected to the upper part of the push plate, a third motor is arranged on one side of the adjusting housing, and an adjusting component is arranged at the output end of the third motor.

[0014] The adjustment component includes a fourth gear arranged at the output end of the third motor, the fourth gear is meshedly connected with a fifth gear, the fifth gear is fixedly connected with a rotating rod close to a side of the adjustment housing, and the rotating rod is fixedly connected to the adjustment plate.

[0015] The transmission mechanism comprises a control housing fixedly connected to the upper part of the experimental box, a first motor is arranged inside the control housing, and a transmission assembly is arranged at the output end of the first motor.

[0016] The transmission assembly includes a screw rod arranged at the output end of the first motor, the screw rod is rotationally connected to the control housing, the screw rod is threadedly connected to a movable plate, and the movable plate is slidingly connected to the control housing.

[0017] A method for using a chronic stress experimental device for experimental small animals comprises the following steps:

[0018] Step 1: Place the experimental animal in the experimental box, turn on the camera, make sure the camera is operating normally, and observe the animal's activities inside the experimental box through the camera;

[0019] Step 2: Adjust the flashing frequency and intensity of the lighting to simulate different visual stimulation environments, observe the behavioral responses of small animals under visual stress, play noises of different frequencies, intensities and types through the buzzer to simulate a noisy environment, observe the behavioral changes of small animals under auditory stress, release the smell of natural enemies through the odor delivery port to simulate the environment where natural enemies exist, and observe the escape behavior and defense mechanism of small animals under olfactory stress;

[0020] Step 3: After the small animals defecate inside the experimental box, the second motor is started, and the push plate is driven by the second motor to move on the surface of the filter plate. During the movement, the small animal feces on the surface of the filter plate are absorbed by the feces suction head;

[0021] Step 4: After the feces are collected, spray disinfectant onto the surface of the filter plate through a disinfection nozzle to disinfect the surface of the filter plate.

[0022] The present invention has the following beneficial effects:

[0023] 1. In the present invention, after the small animals defecate in the experimental box, the cleaning mechanism can drive the push plate to move along the surface of the filter plate. When the push plate moves, the feces suction heads arranged on both sides can effectively absorb the feces on the surface of the filter plate, and the small animal urine remaining on the surface of the filter plate can be cleaned by the absorbent pad cloth arranged at the bottom of the push plate. During the cleaning process, the surface of the filter plate can be effectively disinfected by the disinfection nozzle, which effectively improves the living comfort of the small animals in the experimental box and avoids the influence of non-stress factors on the experimental results.

[0024] 2. In the present invention, the odor delivery port, buzzer and lighting arranged inside the experimental box can respectively provide natural enemy odor stimulation, noise stimulation and visual stimulation to small animals, thereby effectively observing the activity phenomena of small animals under various chronic stimulations, and by starting the transmission mechanism, the movable plate can be driven to move, thereby effectively compressing the living space of small animals and conducting a squeezing stimulation test on the small animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a schematic diagram of the structure of a small animal chronic stress experimental device proposed by the present invention;

[0026] Figure 2 It is a schematic diagram of the internal structure of the experimental box in the present invention;

[0027] Figure 3 It is a schematic diagram of the internal structure of the control housing in the present invention;

[0028] Figure 4 A bottom view of the internal structure of the experimental box of the present invention;

[0029] Figure 5 It is a schematic diagram of the cleaning mechanism structure in the present invention;

[0030] Figure 6 It is a schematic diagram of the internal structure of the push plate in the present invention;

[0031] Figure 7 It is a schematic diagram of the first structure of the adjustment mechanism in the present invention;

[0032] Figure 8 It is a second structural schematic diagram of the adjustment mechanism in the present invention.

[0033] In the figure: 1. Experimental box; 2. Box door; 3. Odor outlet; 4. Ventilation port; 5. Manure collecting trough; 6. Filter plate; 7. Disinfectant tank; 8. Collection box; 9. Water pipe; 10. Water tank; 11. Food pipe; 12. Food trough; 13. Movable plate; 14. Control shell; 15. First motor; 16. Screw rod; 17. Camera; 18. Buzzer; 19. Lighting lamp; 20. Push plate; 21. Manure suction head; 22. Disinfection nozzle; 23. Slide; 24. Transmission rack; 25. Second motor; 26. First gear; 27. Second gear; 28. Third gear; 29. ​​Adjustment shell; 30. Adjustment plate; 31. Third motor; 32. Fourth gear; 33. Fifth gear; 34. Rotating rod. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1

[0036] like Figure 1-Figure 8 As shown, an experimental small animal chronic stress experimental device and an experimental method thereof proposed by the present invention include an experimental box 1, a filter plate 6 is fixedly connected to the lower part of the experimental box 1, a feces collecting trough 5 is arranged at the lower part of the filter plate 6, the feces collecting trough 5 and the experimental box 1 are slidably connected, a transmission mechanism is arranged at the upper part of the experimental box 1, a movable plate 13 is fixedly connected to the lower part of the transmission mechanism, a cleaning mechanism is arranged at the front end of the filter plate 6, the cleaning mechanism includes a slide 23 fixedly connected to the inside of the experimental box 1, a push plate 20 is slidably connected to the inside of the slide 23, a second motor 25 is arranged at the upper part of the push plate 20, a first gear 26 is arranged at the output end of the second motor 25, the first gear 26 is meshedly connected to the second gear 27, the second gear 27 is meshedly connected to the third gear 28, the third gear 28 is meshedly connected to the transmission rack 24 on the right side, and the transmission rack 24 is fixedly connected to the experimental box 1.

[0037] A disinfectant tank 7 is fixedly connected to the upper part of the experimental box 1, and two disinfectant nozzles 22 are arranged at the rear end of the disinfectant tank 7. An adjustment mechanism is arranged on the upper part of the disinfectant nozzle 22. Collection boxes 8 are fixedly connected to both sides of the disinfectant tank 7, and a feces suction head 21 is fixedly connected to the rear end of the collecting box 8. An air pump is arranged inside the feces suction head 21. After the small animal defecates on the filter plate 6, the second motor 25 is started, and the third gear 28 is driven by the second motor 25 to move along the meshing transmission rack 24, thereby driving the push plate 20 to move along the bottom of the filter plate 6. During the movement of the push plate 20, the feces of the small animal are absorbed by the feces suction head 21. After the absorption is completed, the disinfectant is sprayed by the disinfectant nozzle 22 to disinfect the surface of the filter plate 6. A pressurized water pump is arranged inside the disinfectant nozzle 22, and a water-absorbing pad is arranged at the contact part of the bottom of the push plate 20 with the surface of the filter plate 6.

[0038] The adjusting mechanism includes an adjusting shell 29 fixedly connected to the upper part of the push plate 20, a third motor 31 is arranged on one side of the adjusting shell 29, an adjusting component is arranged at the output end of the third motor 31, the adjusting component includes a fourth gear 32 arranged at the output end of the third motor 31, the fourth gear 32 is meshingly connected with a fifth gear 33, the fifth gear 33 is fixedly connected with a rotating rod 34 close to one side of the adjusting shell 29, and the rotating rod 34 is fixedly connected to the adjusting plate 30.

[0039] In this embodiment, after the small animals defecate in the experimental box 1, the feces will remain on the surface of the filter plate 6, and the urine will fall into the feces collecting trough 5 through the filter holes on the filter plate 6. By starting the second motor 25, the first gear 26 can be driven to rotate, and the rotation of the first gear 26 drives the meshing second gear 27 to rotate, and the rotation of the second gear 27 drives the meshing third gear 28 to rotate, and the third gear 28 rotates to move along the meshing transmission rack 24, thereby driving the push plate 20 to move along the surface of the filter plate 6. During the movement, the push plate 20 can ensure the stability of the push plate 20 through the slide groove 23, and when the push plate 20 moves, the feces suction heads 21 arranged on both sides can effectively absorb the feces on the surface of the filter plate 6, and the small animal urine remaining on the surface of the filter plate 6 can be cleaned by the absorbent pad cloth arranged at the bottom of the push plate 20.

[0040] During the cleaning process, the disinfectant in the disinfectant tank 7 can be sprayed out through the disinfectant nozzle 22, thereby disinfecting the surface of the filter plate 6, effectively improving the living comfort of the small animals in the experimental box 1, and avoiding the influence of non-stress factors on the experimental results. The third motor 31 can drive the fourth gear 32 to rotate, and the rotation of the fourth gear 32 drives the fifth gear 33 to rotate. The rotation of the fifth gear 33 can drive the fixedly connected rotating rod 34 to rotate, and the rotation of the rotating rod 34 can drive the fixedly connected adjusting plate 30 to rotate, thereby driving the disinfectant nozzle 22 to rotate and adjust the spraying angle of the disinfectant, thereby effectively improving the disinfection effect.

[0041] Embodiment 2

[0042] like Figure 1-Figure 8 As shown, based on the first embodiment, a water supply pipe 9 is fixedly connected to the left side of the experimental box 1, a water tank 10 is arranged at the lower part of the water supply pipe 9, the water tank 10 is fixedly connected to the experimental box 1, a food pipe 11 is fixedly connected to the left side of the experimental box 1, a food trough 12 is arranged at the lower part of the food pipe 11, the food trough 12 is fixedly connected to the experimental box 1, vents 4 are symmetrically arranged on the left and right sides of the experimental box 1, and a box door 2 is arranged at the front end of the experimental box 1.

[0043] The transmission mechanism includes a control shell 14 fixedly connected to the upper part of the experimental box 1, a first motor 15 is arranged inside the control shell 14, a transmission assembly is arranged at the output end of the first motor 15, the transmission assembly includes a screw rod 16 arranged at the output end of the first motor 15, the screw rod 16 is rotatably connected to the control shell 14, the screw rod 16 is threadedly connected to a movable plate 13, the movable plate 13 and the control shell 14 are slidably connected, an illumination lamp 19 is arranged inside the experimental box 1, a camera 17 is arranged on the right side of the illumination lamp 19, a buzzer 18 is arranged on the rear side of the camera 17, and an odor delivery port 3 is opened on the top of the experimental box 1.

[0044] In this embodiment, the odor delivery port 3, buzzer 18 and lighting lamp 19 arranged inside the experimental box 1 can respectively provide natural enemy odor stimulation, noise stimulation and visual stimulation to the small animals, so as to effectively observe the activity phenomena of the small animals under various chronic stimulations. By starting the first motor 15, the screw rod 16 can be driven to rotate, and the rotation of the screw rod 16 drives the threaded movable plate 13 to move, thereby effectively compressing the living space of the small animals and performing a squeezing stimulation test on the small animals.

[0045] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An experimental device for chronic stress of experimental small animals, comprising an experimental box (1), characterized in that: The experimental box (1) is provided with an illumination lamp (19) inside, a camera (17) is provided on the right side of the illumination lamp (19), a buzzer (18) is provided on the rear side of the camera (17), an odor injection port (3) is provided on the top of the experimental box (1), a filter plate (6) is fixedly connected to the lower part of the experimental box (1), a feces collecting trough (5) is provided at the lower part of the filter plate (6), the feces collecting trough (5) and the experimental box (1) are slidably connected, a transmission mechanism is provided on the upper part of the experimental box (1), a movable plate (13) is fixedly connected to the lower part of the transmission mechanism, and a filter plate (6) is provided at the front end thereof. A cleaning mechanism, the cleaning mechanism comprising a slide groove (23) fixedly connected inside the experimental box (1), a push plate (20) slidably connected inside the slide groove (23), a second motor (25) being arranged on the upper part of the push plate (20), a first gear (26) being arranged at the output end of the second motor (25), the first gear (26) being meshingly connected with a second gear (27), the second gear (27) being meshingly connected with a third gear (28), the third gear (28) being meshingly connected with a transmission rack (24) on the right side, the transmission rack (24) being fixedly connected to the experimental box (1); The upper part of the experimental box (1) is fixedly connected to a disinfectant tank (7), and two disinfectant nozzles (22) are arranged at the rear end of the disinfectant tank (7). An adjustment mechanism is arranged at the upper part of the disinfectant nozzle (22). The two sides of the disinfectant tank (7) are fixedly connected to a collection box (8), and the rear end of the collection box (8) is fixedly connected to a feces suction head (21). An air pump is arranged inside the feces suction head (21). After the small animal defecates on the filter plate (6), the second motor (25) is started, and the third gear (28) is driven by the second motor (25) to move along the meshing transmission rack (24), thereby driving the push plate (20) to move along the bottom of the filter plate (6). During the movement of the push plate (20), the feces of the small animal are absorbed by the feces suction head (21). After the absorption is completed, the disinfectant is sprayed on the surface of the filter plate (6) by the disinfectant nozzle (22) to disinfect.

2. The experimental device for chronic stress of small animals according to claim 1, characterized in that: A water supply pipe (9) is fixedly connected to the left side of the experimental box (1), a water tank (10) is arranged at the lower part of the water supply pipe (9), and the water tank (10) is fixedly connected to the experimental box (1).

3. The experimental device for chronic stress of experimental small animals according to claim 1, characterized in that: A food pipe (11) is fixedly connected to the left side of the experimental box (1), a food trough (12) is provided at the lower part of the food pipe (11), and the food trough (12) is fixedly connected to the experimental box (1).

4. The experimental device for chronic stress of experimental small animals according to claim 1, characterized in that: The experimental box (1) is symmetrically provided with ventilation openings (4) on both sides thereof, and the front end of the experimental box (1) is provided with a box door (2).

5. The experimental device for chronic stress of experimental small animals according to claim 1, characterized in that: The adjustment mechanism comprises an adjustment housing (29) fixedly connected to the upper part of the push plate (20), a third motor (31) is arranged on one side of the adjustment housing (29), and an adjustment component is arranged at the output end of the third motor (31).

6. The experimental device for chronic stress of experimental small animals according to claim 5, characterized in that: The adjustment component comprises a fourth gear (32) arranged at the output end of the third motor (31), the fourth gear (32) being meshingly connected with a fifth gear (33), the fifth gear (33) being fixedly connected with a rotating rod (34) on a side close to the adjustment housing (29), and the rotating rod (34) being fixedly connected to the adjustment plate (30).

7. The experimental device for chronic stress of experimental small animals according to claim 1, characterized in that: The transmission mechanism comprises a control housing (14) fixedly connected to the upper part of the experimental box (1), a first motor (15) is arranged inside the control housing (14), and a transmission assembly is arranged at the output end of the first motor (15).

8. The experimental device for chronic stress of experimental small animals according to claim 7, characterized in that: The transmission assembly comprises a screw rod (16) arranged at the output end of the first motor (15), the screw rod (16) being rotationally connected to the control housing (14), the screw rod (16) being threadedly connected to a movable plate (13), and the movable plate (13) being slidingly connected to the control housing (14).

9. The method for using the experimental small animal chronic stress experimental device according to claim 1, characterized in that: The following steps are involved: Step 1: Place the experimental animal into the experimental box (1), turn on the camera (17), ensure that the camera (17) operates normally, and observe the activities of the animal inside the experimental box (1) through the camera (17); Step 2: Adjust the flashing frequency and intensity of the lighting lamp (19) to simulate different visual stimulation environments, observe the behavioral responses of the small animals under visual stress, play noises of different frequencies, intensities and types through the buzzer (18) to simulate a noisy environment, observe the behavioral changes of the small animals under auditory stress, release the smell of natural enemies through the smell delivery port (3) to simulate the environment where natural enemies exist, and observe the escape behavior and defense mechanism of the small animals under olfactory stress; Step 3: After the small animals defecate inside the experimental box (1), the second motor (25) is started, and the push plate (20) is driven by the second motor (25) to move on the surface of the filter plate (6), and the small animal feces on the surface of the filter plate (6) are absorbed by the feces suction head (21) during the movement; Step 4: After the feces are collected, a disinfectant is sprayed onto the surface of the filter plate (6) through a disinfectant nozzle (22) to disinfect the surface of the filter plate (6).

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