Application and method of Cinnamomum longepaniculatum extract delta-terpilenol in prevention and treatment of agricultural fungal diseases and postharvest rot of fruits

Abstract

The invention discloses application and method of a cinnamomum longepaniculatum extract delta-terpilenol in prevention and treatment of agricultural fungal diseases and fruit postharvest rot, and belongs to the technical field of plant-source fungus bacteriostasis. Based on the efficient bacteriostatic activity of delta-terpilenol, the delta-terpilenol has a significant effect on botrytis cinerea causing fruit postharvest rot, and the delta-terpilenol has a significant effect on preventing and treating agricultural fungal diseases and fruit postharvest rot. The bactericidal composition has high-efficiency antibacterial activity on various agricultural pesticides, and rhizoctonia solani, pestalotiopsis, sweet potato coracocystis and the like which cause agricultural plant diseases, can replace chemical pesticides to prevent and treat crop diseases, has the advantages of low toxicity, low residue and environmental friendliness, can reduce the research and development cost of agricultural bactericides, saves time, improves the production of pesticides, and has wide application prospects. A wider choice is provided for a plant-derived fungus bacteriostatic agent.

Description

Technical Field

[0001] The present invention discloses an application and method of δ-pineneol of camphor tree extract in preventing and controlling agricultural fungal diseases and post-harvest fruit decay, and specifically relates to an application of δ-pineneol of camphor tree extract in preventing and controlling agricultural fungal diseases and post-harvest fruit decay, a control agent and a preservative used in the application, and a method for respectively inhibiting the activity of agricultural pathogenic fungi and post-harvest fruit decay fungi by using the control agent and the preservative, and belongs to the technical field of plant-derived fungal inhibition. Background Art

[0002] Plant fungal diseases seriously threaten the healthy development of agriculture and food safety. There are many types of fungal diseases, accounting for more than 70-80% of all plant diseases. Fungal diseases are widely distributed in my country. They not only cause harm in the field, but also harm fruits due to their latent infection characteristics, which can reduce yields and make fruits lose their commercial value. The losses are huge and seriously affect the safety of fruit production. These fungal diseases usually cause anthracnose, ring rot, powdery mildew, rot, black spot disease, etc. in plants. These serious plant diseases cause up to 40% loss of global crop yields each year, causing more than $220 billion in losses to the global economy, and have seriously threatened global food security and economic development. Therefore, research and development of new pesticides with high efficiency, low toxicity and low residue that can effectively control plant pathogens has become one of the primary goals of pesticide creation.

[0003] Camphor oil contains extremely rich monoterpene natural products and their analogs, including α-terpineol, β-terpineol, γ-terpineol, α-pinene, camphor, etc. Most of these monoterpene natural products and their analogs can be used to inhibit agricultural pathogenic fungi, but as the fungal resistance increases, the activity of these monoterpene natural products and their analogs gradually decreases. Therefore, it is urgent to develop other natural products to solve this problem. Natural camphor extract δ-terpineol is an isomer of α-terpineol, and the main structural difference lies in the different positions of carbon-carbon double bonds and hydroxyl groups. δ-terpineol is a scarce natural plant product. At present, with the improvement of process technology, it has been solved to obtain high-purity δ-terpineol and other natural products from plant essential oils, but due to its lack of application, the relevant research on δ-terpineol and these natural extracts has been seriously lagging behind, especially the research on agriculture is very limited.

[0004] In the prior art, Wei Qin et al. studied the antibacterial activity of camphor oil against rice blast pathogen, pine red blight pathogen, corn sheath blight pathogen, corn curvature pathogen, wheat scab pathogen and gloeosporium anthracis in "Inhibitory effect of camphor oil on plant pathogenic fungi" (Chinese Journal of Oil Crops, March 28, 2006 (1): 63-66). In a method for inhibiting fungi with camphor leaf essential oil disclosed in Chinese patent CN110150333A, it is mentioned that camphor leaf essential oil has obvious antibacterial effect against Fusarium oxysporum, Thielavia septoria and Phomopsis spp., and GC-MS analysis of camphor leaf essential oil shows that the contents of compounds such as 1,8-cineole, β-phellandrene, α-pinene, α-pinene, L-β-pinene and terpineol-4 are relatively high, but δ-pinene, as a rare natural plant product, has not been identified in the camphor leaf essential oil.

[0005] It can be seen that although existing studies have shown that most extracts of camphor oil have the effect of inhibiting the activity of plant pathogenic fungi, there is currently no report that δ-pineneol, a camphor oil extract, has anti-plant pathogenic fungi and mold activity. Therefore, research and development of the application of δ-pineneol in agriculture is expected to reduce the research and development cost of agricultural fungicides, save time, and increase the output of pesticide creation. Summary of the invention

[0006] The present invention aims to provide an application and method of δ-pineneol, a camphor tree extract, in preventing and controlling agricultural fungal diseases and post-harvest fruit decay. The antibacterial activity of δ-pineneol in agricultural fungal diseases and post-harvest fruit decay is studied, giving the scarce natural plant product δ-pineneol a broader application prospect, in order to replace chemical pesticides to prevent and control crop diseases. The invention has the advantages of low toxicity, low residue and environmental friendliness, and can also be used as a preservative for post-harvest fruit, providing a wider range of choices for plant-derived fungal inhibitors.

[0007] The present invention is achieved through the following technical solutions:

[0008] Technical solution 1:

[0009] Provided is the use of δ-pineneol from camphor tree extract in preventing and controlling agricultural fungal diseases, wherein the agricultural fungal diseases include gray mold, sweet potato black spot, damping-off or verticillium wilt, and the structure of the δ-pineneol is as follows:

[0010]

[0011] The control is achieved by utilizing delta-pinene alcohol to inhibit the activity of agricultural pathogenic fungi, and the agricultural pathogenic fungi include: Fusarium, Rhizoctonia solani, Alternaria, Pyrospora, Aspergillus niger, Polytrichomoniasis, Sclerotinia sclerotiorum, Verticillium fulvicinum and Cochliobolus graminearum.

[0012] Furthermore, the Fusarium includes Fusarium graminearum, Fusarium graminearum-1, Fusarium graminearum-2, Fusarium solani (Fusarium solani-1, Fusarium solani-2), Fusarium oxysporum and Fusarium oxysporum (Fusarium oxysporum cucurbit-specific type, Fusarium oxysporum watermelon-specific type, Fusarium oxysporum wilt-specific type).

[0013] The polyhistosporium includes polyhistosporium microsporum and polyhistosporium mangiferum.

[0014] The Alternaria species include Alternaria apple.

[0015] Technical solution 2:

[0016] Provided is a control agent for use in the above application, that is, a control agent for the above agricultural fungal diseases, wherein the control agent contains at least the above effective amount of δ-pineneol from camphora extract.

[0017] Technical solution three:

[0018] A method for inhibiting the activity of agricultural pathogenic fungi using the above-mentioned control agent is provided.

[0019] Technical solution 4:

[0020] Provided is the use of δ-pineneol from camphor tree extract in preventing and controlling post-harvest fruit decay. The prevention and control is achieved by using δ-pineneol to inhibit the activity of post-harvest fruit decay fungi. The structure of the δ-pineneol is as follows:

[0021]

[0022] The fruit post-harvest decay fungi include Botrytis cinerea, such as Botrytis cinerea (gray mold) and Botrytis cinerea (strawberry gray mold).

[0023] Technical solution five:

[0024] Provided is a preservative for use in the above application, that is, a preservative for treating post-harvest fruit decay, wherein the preservative contains at least the above effective amount of δ-pineneol from camphora oleifera extract.

[0025] Technical solution six:

[0026] Provided is a method for inhibiting the activity of post-harvest decay-causing fungi of fruits by utilizing the preservative agent.

[0027] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0028] (1) The present invention is based on the high antibacterial activity of δ-pinene alcohol, especially for Botrytis cinerea, which causes post-harvest fruit decay, the semi-inhibitory activity of δ-pinene alcohol can reach 463.07 ppm, providing a new use of δ-pinene alcohol in preventing and controlling post-harvest fruit decay. Based on the new use, a preservative containing δ-pinene alcohol is further provided, as well as a method for using the preservative to inhibit the activity of post-harvest fruit decay fungi.

[0029] (2) The present invention is based on the high antibacterial activity of δ-pinene alcohol against Rhizoctonia solani, Pseudomonas aeruginosa, and Aspergillus niger, which cause agricultural plant diseases. Among them, the in vitro activity EC50 for Rhizoctonia solani can reach 594.36 ppm; the EC50 for Pseudomonas microsporus and Pseudomonas mangifera can reach 615.65 ppm and 600.41 ppm, respectively; the EC50 for Verticillium foetida can reach 556.31 ppm; and the in vitro activity EC50 for Aspergillus niger can reach 429.31 ppm. Thus, a new use of δ-pinene alcohol in preventing and controlling agricultural fungal diseases is provided. Based on this use, a control agent containing δ-pinene alcohol is further provided, as well as a method for using the control agent to inhibit the activity of agricultural pathogenic fungi.

[0030] (3) The δ-pinene alcohol involved in the present invention belongs to a kind of natural camphor extract. It has been proved by experiments that the δ-pinene alcohol has obvious antibacterial effects on Rhizoctonia solani, Pseudomonas microsporus, Botrytis cinerea, Verticillium erythrorhizium and Aspergillus niger. When the concentration of δ-pinene alcohol is 1000ppm, the inhibitory effect on pathogenic fungi is most obvious. δ-pinene alcohol can inhibit or destroy the formation of biofilm and cell wall. When the permeability of cell wall increases or is damaged, the dissolved substances leak out of the cell, resulting in the death of bacteria. Therefore, it can replace chemical pesticides to prevent and control crop diseases, and has the advantages of low toxicity, low residue and environmental friendliness. It can reduce the research and development cost of agricultural fungicides, save time, and increase the output of pesticide creation, providing a wider range of choices for plant-derived fungicide. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The graph shows the antibacterial effects of 16 camphor tree extracts on Botrytis cinerea (gray mold) (127).

[0032] Figure 2 This is a graph showing the antibacterial effects of 16 camphor tree extracts on Fusarium (strawberry) (26-1).

[0033] Figure 3 The graph shows the antibacterial effects of 16 camphor tree extracts on Fusarium graminearum (Gibberellus) (54).

[0034] Figure 4 The graph shows the antibacterial effects of 16 camphor tree extracts on Rhizoctonia solani (146).

[0035] Figure 5 This is a graph showing the antibacterial effects of 16 camphor tree extracts on Fusarium solani-1 (001).

[0036] Figure 6 The graph shows the antibacterial effects of 16 camphor tree extracts on Fusarium solani-2 (143).

[0037] Figure 7 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Pseudomonas microsporum (159).

[0038] Figure 8 The graph shows the antibacterial effects of 16 camphor tree extracts on Botrytis cinerea (153).

[0039] Fig. 9 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Pseudomonas aeruginosa (59).

[0040] Fig.10 This is a graph showing the antibacterial effects of 16 Cinnamomum camphora extracts on Fusarium oxysporum var. gourdii-specific type (109).

[0041] Fig.11 The graph shows the antibacterial effects of 16 camphor tree extracts on Fusarium oxysporum (solanegrum root rot) (92).

[0042] Fig.12 This is a graph showing the antibacterial effects of 16 Cinnamomum camphora extracts on Fusarium oxysporum var. melonensis type (108).

[0043] Fig.13 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on the specialized type of Fusarium oxysporum (107).

[0044] Fig.14 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Fusarium solani (tomato root rot) (77).

[0045] Fig.15 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Fusarium oxysporum (cucumber wilt pathogen) (106).

[0046] Fig.16 This is a graph showing the antibacterial effects of 16 Cinnamomum camphora extracts on Fusarium solani (peach tree)-1 (86).

[0047] Fig.17 This is a graph showing the antibacterial effects of 16 camphor extracts on Fusarium solani (peach tree)-2 (85).

[0048] Fig.18 The antibacterial effect diagram of 16 camphor extracts on Sclerotinia sclerotiorum (tobacco) (160).

[0049] Fig.19The graph shows the antibacterial effects of 16 camphor tree extracts on Alternaria alternata (tobacco brown spot disease-2) (156).

[0050] Fig. 20 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Verticillium fulvicinum (120).

[0051] Fig.21 The graph shows the antibacterial effects of 16 camphor tree extracts on Alternaria alternata (tobacco brown spot disease-1) (155).

[0052] Fig. 22 The graph shows the antibacterial effects of 16 camphor tree extracts on Alternaria officinalis (4).

[0053] Fig.23 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Aspergillus (apple rot) (42).

[0054] Fig.24 The graph shows the antibacterial effects of 16 Cinnamomum camphora extracts on Helicobacter graminearum (wheat root rot) (157).

[0055] Fig.25 The graph shows the antibacterial effects of 16 camphor extracts on sweet potato long-beaked fungus (sweet potato black spot) (72).

[0056] Fig.26 The graph shows the antibacterial effects of 16 camphor tree extracts on Fusarium oxysporum (a pathogenic bacterium of Albizzia julibrissin) (110).

[0057] Fig. 27 This is a diagram showing the inhibitory effect on leaves infected with gray mold in the Blank group.

[0058] Fig.28 This is a graph showing the inhibitory effect of 500 ppm δ-pineneol on leaves infected with gray mold.

[0059] Fig.29 This is a graph showing the inhibitory effect of 1000 ppm δ-pineneol on leaves infected with gray mold.

[0060] Fig.30 This is a diagram showing the inhibitory effect of the oxazolidinone group on leaves infected with gray mold. DETAILED DESCRIPTION

[0061] The invention objectives, technical solutions and beneficial effects of the present invention are further described in detail below.

[0062] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the claimed invention, and unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs.

[0063] The present invention provides an application of δ-pineneol from camphora extract in preventing and controlling agricultural fungal diseases and post-harvest fruit decay, and also provides control agents and preservatives used in these applications, as well as methods for using these control agents and preservatives to inhibit the activity of agricultural pathogenic fungi and post-harvest fruit decay fungi, respectively. The following is a further detailed overview of the technical solution of the present invention:

[0064] With regard to the application of δ-pineneol of camphor extract in preventing and controlling agricultural fungal diseases, the present invention utilizes δ-pineneol to inhibit the activity of agricultural pathogenic fungi to achieve the prevention and control of agricultural fungal diseases, wherein the δ-pineneol has the following structural formula:

[0065]

[0066] The agricultural pathogenic fungi involved in the present invention include but are not limited to Fusarium, Rhizoctonia solani, Alternaria, Pyrospora, Echinopsora, Pseudomonas, Sclerotinia, Verticillium sclerotiorum and Corytomyces graminearum. Fusarium includes Fusarium graminearum, Fusarium graminearum-1, Fusarium graminearum-2, Fusarium solani (Fusarium solani-1, Fusarium solani-2), Fusarium oxysporum and Fusarium oxysporum (Fusarium oxysporum cucurbita specialized type, Fusarium oxysporum watermelon specialized type, Fusarium oxysporum wilt specialized type); Pseudomonas sclerotiorum includes Pseudomonas microsporus and Pseudomonas mangoe; Alternaria includes Alternaria applee.

[0067] The agricultural fungal diseases involved in the present invention include but are not limited to gray mold, sweet potato black spot, damping-off or verticillium wilt.

[0068] With regard to the application of δ-pinene alcohol extracted from camphor tree extract in preventing and controlling post-harvest fruit decay, the present invention utilizes δ-pinene alcohol to inhibit the activity of post-harvest fruit decay fungi to achieve the prevention and control of post-harvest fruit decay, wherein the δ-pinene alcohol is the same as above.

[0069] The fruit post-harvest decay fungi involved in the present invention include but are not limited to Botrytis cinerea, such as Botrytis cinerea (gray mold) and Botrytis cinerea (strawberry gray mold).

[0070] Since the δ-pinene alcohol of the present invention has obvious antibacterial effects on the above-mentioned pathogens, and δ-pinene alcohol is a natural camphor tree extract, δ-pinene alcohol can be used as a plant-derived control agent or preservative, which can replace existing chemical agents and has the advantages of low toxicity, low residue and environmental friendliness.

[0071] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0072] The natural camphor extract δ-pinpineol used in the following examples was provided by Yibin Shiping Spice Co., Ltd.; the test strains were all provided by Chengdu Xinchaoyang Crop Science Co., Ltd.

[0073] Example 1: Test on the activity of δ-terpineol in inhibiting agricultural pathogenic fungi and post-harvest fruit decay fungi

[0074] This example verifies the control effects of 16 natural camphor extracts including δ-pineneol on 26 plant pathogenic fungi. The 16 natural camphor extracts are shown in Table 1 below, and the 26 plant pathogenic fungi are shown in Table 2 below.

[0075] Table 1: 16 main extracts of natural camphor

[0076] serial number name serial number name 1 Eucalyptol 9 α-Terpineol 2 Crude Camphor Oil 10 White camphor oil 3 Eucalyptus Oil 11 α-Pyrene 4 Myrcene 12 Naphtha 5 Whitening oil 13 Terpinen-4-ol 6 Sabinene 14 Terpinolene 7 β-Caryophyllene 15 Natural Camphor 8 γ-Terpineol 16 δ-Pinpineol

[0077] Table 2: 26 plant pathogenic fungi

[0078] serial number Fungus name serial number Fungus name 127 Botrytis cinerea (grey mold) 109 Fusarium oxysporum 26-1 Fusarium (Strawberry) 92 Fusarium oxysporum (eggplant root rot) 54 Fusarium graminearum (Gibberella) 108 Fusarium oxysporum 146 Rhizoctonia solani 110 Fusarium oxysporum (pathogenic bacteria of Albizzia julibrissin) 001 Fusarium-1 107 Fusarium wilt type 143 Fusarium-2 72 Sweet potato black spot 159 Pseudodiscus microsporus 77 Solanum solani (solanum root rot) 153 Botrytis cinerea 106 Fusarium oxysporum (Cucumber wilt pathogen) 59 Pseudodiscus mangifera 86 Solanum disease sickle (peach tree) - 1 85 Solanum sickle (peach tree) -2 160 Sclerotinia sclerotiorum (tobacco) 155 Alternaria alternata (tobacco brown spot disease-1) 120 Verticillium fulvei 156 Alternaria alternata (tobacco brown spot disease-2) 4 Alternaria apple 42 Pyrospora (apple rot) 157 Helicobacterium graminearum (wheat root rot)

[0079] The plant pathogenic fungi used in this embodiment are strains stored at -80°C in the laboratory. The mycelium growth rate method is used. The culture medium used for all plant pathogenic fungi in the laboratory is potato agar glucose medium (PDA for short). PDA medium formula: 200g potato, 20g glucose, 15g agar, 1000mL distilled water. The prepared PDA medium is sterilized at 121°C for 20 minutes and cooled for use.

[0080] Experimental method (hyphae growth rate method):

[0081] 1. Cultivate the plant pathogens on PDA plates at 25°C for several days to activate them;

[0082] 2. Heat and melt the PDA culture medium, and after cooling, add 16 kinds of Cinnamomum camphora extract mother solutions to prepare drug-containing plates;

[0083] 3. Use a cork puncher to punch a round bacterial cake (4 mm in diameter) at the edge of the hyphae of each strain that has been cultured for several days (the growth conditions should be as consistent as possible), and then use a bacteria needle to pick it to the center of the drug-containing plate. The above operations are all performed on a sterile clean bench, and then the culture dish is inverted and cultured in an incubator at 25°C for several days;

[0084] 4. After the blank control group is fully grown, observe the growth of mycelium in the drug-treated plate group, measure the diameter using the cross method, process the data, and calculate the mycelium growth inhibition rate (Percentages of Growth Inhibition, PGI);

[0085] 5. Mycelial growth inhibition rate (%) = (control mycelial diameter - treated mycelial diameter) / (control mycelial diameter - mycelial disc diameter) × 100;

[0086] 6. Each treatment was repeated three times.

[0087] The experimental results are shown in Figures 1 to 26 as follows, which are the bacteriostatic effect diagrams of 16 natural Cinnamomum longepaniculatum extracts including δ-terpineol against 26 plant pathogenic fungi, Figures 1 to 26 where BL represents the blank group, 9 ml of PDA + 1 ml of sterile water; Rong represents the solvent group, 9 ml of PDA + 1 ml of solvent; Zheng represents the currently commercially available bacteriostatic agent (hymexazol), and the numbers 1 - 16 correspond to the numbers of the 16 natural Cinnamomum longepaniculatum extracts in Table 1 in sequence, and the number 16 is the δ-terpineol group.

[0088] Furthermore, according to the mycelial growth of δ-terpineol against 26 plant pathogenic fungi, the corresponding mycelial growth inhibition rate was calculated, as shown in Tables 3 to 5.

[0089] Table 3: Summary of the bacteriostatic effects of δ-terpineol against Botrytis cinerea, Fusarium spp., and Pestalotiopsis spp.

[0090]

[0091] Note: The concentration of δ-terpineol is 1000 ppm; the bacteriostatic effect of grade A indicates that the bacteriostatic rate is 80 - 100%; grade B indicates 60 - 79%; grade C indicates 40 - 59%; grade D indicates < 40%; - indicates that the bacteriostatic rate is negative.

[0092] Table 4: Summary of the bacteriostatic effects of δ-terpineol against Fusarium spp.

[0093]

[0094] Note: The concentration of δ-terpineol is 1000 ppm; the bacteriostatic effect of grade A indicates that the bacteriostatic rate is 80 - 100%; grade B indicates 60 - 79%; grade C indicates 40 - 59%; grade D indicates < 40%; - indicates that the bacteriostatic rate is negative.

[0095] Table 5: Summary of the bacteriostatic effects of δ-terpineol against Fusarium spp., Alternaria alternata, Verticillium albo-atrum, etc.

[0096]

[0097]

[0098] Note: The concentration of δ-terpineol is 1000ppm; the antibacterial effect of grade A means the antibacterial rate is 80-100%; grade B means 60-79%; grade C means 40-59%; grade D means <40%; - means the antibacterial rate is negative.

[0099] After initially screening the antibacterial activity of δ-pineneol against 26 agricultural pathogenic fungi, we selected agricultural pathogens with better inhibitory effects, tested the antibacterial activity of samples of different concentrations against agricultural pathogens, and calculated the corresponding EC50.

[0100] Table 6: Summary of EC50 of δ-pinpineol against Botrytis cinerea, Rhizoctonia solani, Polytrichomoniasis spp., Verticillium fulviciensis and Aspergillus niger

[0101]

[0102] Note: - indicates that there is no antibacterial activity test for the concentration corresponding to the pathogen.

[0103] Based on the above experiments, it can be known that the natural camphor extract δ-pineneol of the present invention has certain antibacterial activity against a variety of agricultural plant pathogenic fungi and fruit post-harvest decay fungi. The semi-inhibitory activity of δ-pineneol against Botrytis cinerea (gray mold) can reach 463.07ppm; the in vitro activity EC50 for Rhizoctonia solani can reach 594.36ppm; the EC50 for Pseudomonas microsporus and Pseudomonas mangifera can reach 615.65ppm and 600.41ppm respectively; the EC50 for Verticillium erythrorhizium can reach 556.31ppm; the in vitro activity EC50 for Pseudomonas aeruginosa can reach 429.31ppm, and δ-pineneol is separated from natural camphor and has good safety.

[0104] The present invention further proves through experiments that the natural camphor extract δ-pineneol has a certain preventive effect on plants infected with gray mold, sweet potato black spot, damping-off and Verticillium wilt, and therefore has a prospect for development and application in the field of agricultural fungal disease prevention and control. At the same time, through fruit preservation experiments, it is proved that the natural camphor extract δ-pineneol has a certain preventive effect on post-harvest fruit decay. Take the potted plant experiment infected with gray mold as an example, see Example 2 for details.

[0105] Example 2: Effect of δ-pinpineol on the prevention and treatment of gray mold infection

[0106] Experimental method: Take leaves of normal-sized potted plants and soak them in different test drug groups for 10 seconds. Then take out the leaves, dry them naturally, and place them face up in a culture dish covered with filter paper. Soak the filter paper with ultrapure water (UP water) to keep it moist. Connect a 4mm diameter bacterial cake to the surface of the dried leaves inversely. Use an artificial climate box with humidity >95% and 25℃, and add UP water to keep the leaves moist. Investigate the disease situation after 72 hours. Repeat each experiment 3 times.

[0107] Experimental drug groups: (1) Blank group (leaves soaked in up water); (2) 500 ppm δ-pineneol group (leaves soaked in 500 ppm δ-pineneol); (3) 1000 ppm δ-pineneol group (leaves soaked in 1000 ppm δ-pineneol); (4) 500 ppm oxadiazon group (leaves soaked in 500 ppm oxadiazon).

[0108] The experimental results are shown in Figures 27 to 30 As shown in the figure, it can be seen that compared with the Blank group, the 500ppm δ-pineneol group, the 1000ppm δ-pineneol group and the oxazolidinone group all have inhibitory activity on leaves infected with gray mold (Botrytis cinerea), among which the 1000ppm δ-pineneol group has the best inhibitory effect, with an inhibition rate of 100%, and the inhibition rate of the 500ppm δ-pineneol group can also reach 48.3%, which is almost equivalent to the inhibitory effect of the oxazolidinone group (the inhibition rate is 50.2%).

[0109] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Any simple modification or equivalent change made to the above embodiment based on the technical essence of the present invention shall fall within the protection scope of the present invention.

Claims

1. Application of δ-pineneol from camphor extract in preventing and controlling agricultural fungal diseases, characterized in that: The agricultural fungal diseases include gray mold, sweet potato black spot, damping-off or Verticillium wilt, and the structure of the delta-terpineol is as follows: 。 2. The use according to claim 1, characterized in that: The control is achieved by utilizing delta-pinene alcohol to inhibit the activity of agricultural pathogenic fungi, and the agricultural pathogenic fungi include: Fusarium, Rhizoctonia solani, Alternaria, Pyrospora, Aspergillus niger, Polytrichomoniasis, Sclerotinia sclerotiorum, Verticillium fulvicinum and Cochliobolus graminearum.

3. The use according to claim 2, characterized in that: The Fusarium species include Fusarium graminearum, Fusarium graminearum-1, Fusarium graminearum-2, Fusarium solani, Fusarium oxysporum and Fusarium oxysporum.

4. The use according to claim 2, characterized in that: The polyhistosporium includes polyhistosporium microsporum and polyhistosporium mangiferum.

5. The use according to claim 2, characterized in that: The Alternaria species include Alternaria apple.

6. A control agent for use as claimed in any one of claims 1 to 5.

7. A method for inhibiting the activity of agricultural pathogenic fungi using the control agent according to claim 6.

8. Application of δ-pineneol from camphor extract in preventing and controlling postharvest fruit decay, characterized in that: The control is achieved by using δ-terpineol to inhibit the activity of post-harvest decay fungi in fruits. The structure of the δ-terpineol is as follows: ， The post-harvest fruit decay fungi include Botrytis cinerea.

9. An antiseptic for use as claimed in claim 8.

10. A method for inhibiting the activity of post-harvest decay-causing fungi of fruits using the preservative according to claim 9.

Images