Composite seaweed, feed additive, application and method for reducing methane emission of ruminant rumen
By adding complex seaweeds, including salt algae and Chlorella, the problem of methane emissions in ruminants was solved in ruminant feed, achieving significant methane emission reduction effects and ensuring food safety and sustainability.
Patent Information
- Application Number
- CN202510109670.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-23
- Publication Date
- 2025-04-29
AI Technical Summary
Ruminant methane emissions in ruminants are an important source of the carbon footprint of the animal husbandry industry. The existing technology is difficult to effectively reduce methane emissions, and some additives accumulate in animals, which poses food safety risks.
Compound seaweeds are used as feed additives, including salt algae and Chlorella, and are mixed and treated in specific proportions to make wettable powders, emulsions, pastes, granules or solutions, and added to the base feed.
It significantly reduces the production of rumen methane in ruminants, has significant methane emission reduction effect, and compound seaweeds are natural, safe and sustainable, and will not have a negative impact on animal health.
Abstract
Description
Technical Field
[0001] The present application relates to the field of ruminant feed, and in particular, to a composite seaweed, a feed additive, an application thereof, and a method for reducing rumen methane emission of ruminants. Background Art
[0002] Ruminants such as cattle and sheep produce about 800 million tons of methane each year, accounting for about 28% of methane emissions from human activities. Methane emissions from ruminants have become a serious challenge to the sustainable development of agriculture and animal husbandry. Marine algae are rich in a variety of life-active substances and can be used as ruminant feed additives. Natural products such as brominated compounds, seaweed polysaccharides, polyphenols and polyunsaturated fatty acids in certain algae can reduce the abundance of methane-producing microorganisms in the rumen of cattle and sheep, and the efficiency of inhibiting methane emissions can be as high as 90%.
[0003] Methane emissions from animal husbandry mainly come from three aspects: first, during the fermentation of feed stacking; second, during the feces treatment process; and third, methane produced by fermentation in the rumen of ruminants is emitted into the atmosphere through rumination. The first two sources can be collected as clean energy by setting up a closed fermentation system, but it is difficult to completely collect methane emitted by rumination during the grazing process. Therefore, ruminant gastrointestinal methane emission reduction technology is an important topic for methane emission reduction in animal husbandry. Methane emission reduction seaweed feed additives have been developed to help reduce carbon emissions in animal husbandry and achieve green feeding.
[0004] At present, there are three technical strategies for reducing methane emissions from the gastrointestinal tract of ruminants. The first is to increase concentrate feed, adjust daily ration nutrition, and improve feed quality; the second is to artificially add probiotics to change the structure of gastrointestinal microbial communities; the third is to specifically add methane inhibitors to feed. The first strategy is costly and the methane reduction efficiency is less than 50%. The discontinuous change in the ratio of concentrate to roughage in the feed may also lead to a series of negative hazards such as acute or subacute acidosis of the rumen, disturbance of digestive tract microbial flora, hoof disease, ketosis, etc. The technology of the second strategy is not yet mature, and the long-term effect is unknown. Specially adding methane inhibitors to feed is considered to be the most cost-effective methane reduction strategy. Vegetable oils containing unsaturated fatty acids, plant extracts containing tannins and saponins, 3-nitrooxypropanol, nitrates, etc. have been tried as ruminant feed additives, but their gastrointestinal methane reduction efficiency is rarely higher than 50%, and these substances accumulate in ruminants, causing concerns about food safety risks. Summary of the invention
[0005] In order to make up for the above shortcomings, the present application provides an application of composite seaweed in reducing methane emissions in the rumen of ruminants, aiming to improve the problem of methane production in the rumen of ruminants.
[0006] An embodiment of the present application provides a composite seaweed, including Dunaliella and Chlorella.
[0007] In a preferred embodiment of the present invention, the ratio of the Dunaliella to the Chlorella is 1:1. The carotene content of the Dunaliella is greater than or equal to 3%; the protein content of the Chlorella is greater than or equal to 57.5%.
[0008] In a preferred embodiment of the present invention, the composite seaweed is used as a ruminant feed additive. The composite seaweed is added to the basic feed in the form of a wettable powder, emulsion, paste, granule or solution, and the feed additive is obtained by mixing. The composite seaweed granules are prepared by the following method: obtaining the composite seaweed, desalting, and drying. The composite seaweed is mixed in a certain proportion, and processed by drying, crushing and other processes to prepare composite seaweed powder. The amount of composite seaweed powder added to ruminant feed can be adjusted according to factors such as the type, weight, and growth stage of the ruminant.
[0009] On the one hand, as a ruminant feed additive, the feed additive is added in an amount of 0.2% to 10% in the feed.
[0010] In another aspect, a method of reducing rumen methane emissions from a ruminant comprises adding a feed additive to a feed for the ruminant.
[0011] In a preferred embodiment of the present invention, the amount of the feed additive added to the feed is 1% to 5%; specifically, the amount of the feed additive added to the feed is 1.5% or 4.5%.
[0012] In a preferred embodiment of the present invention, the feed includes a basic feed and a composite seaweed. The composite seaweed is in the form of one or more of a fresh product, a dry product or a freeze-dried powder. The composite seaweed is prepared by salt pan aquaculture concentration, separation, desalination and drying to obtain a fresh seaweed product, and the fresh seaweed product is processed to obtain a dry product powder or a freeze-dried powder.
[0013] In a preferred embodiment of the present invention, the amount of the composite seaweed added to the feed is calculated on a dry weight basis and is expressed in weight percentage. The basic feed is selected from feed suitable for ruminants available in the prior art, and the basic feed is selected from corn silage.
[0014] In a preferred embodiment of the present invention, the composite seaweed is used as a feed additive to reduce the production of methane in the gastrointestinal tract of ruminants.
[0015] In a preferred embodiment of the present invention, the composite seaweed is used in the preparation of feed with the effect of reducing gastrointestinal methane production in ruminants; the amount of the seaweed added to the feed is 0.2% to 10%. The ruminants include cattle, sheep and deer.
[0016] Beneficial effects: This application provides an application of composite seaweed in ruminant rumen methane emission reduction. The present invention adds composite seaweed to basic feed, and experimental studies have shown that it has a good gastrointestinal methane emission reduction effect. The composite seaweed used is Dunaliella and Chlorella. Dunaliella and Chlorella can utilize my country's abundant salt field breeding resources, have mature industrial breeding technology, and are easy to obtain resources; the raw materials used in the present invention are widely available, with low cost, and significant gastrointestinal methane emission reduction effect. The present invention has important value and significance for my country's animal husbandry methane emission reduction technology, and has great potential for industrial application.
[0017] Composite seaweed can effectively reduce the production of rumen methane, with significant emission reduction effects. In addition, composite seaweed is natural, safe, and sustainable, and will not have a negative impact on the health of ruminants. The composite seaweed preparation method is simple, low-cost, and easy to promote and apply. DETAILED DESCRIPTION
[0018] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0019] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.
[0020] The technical solutions in the embodiments of the present application will be described below in combination with the embodiments of the present application.
[0021] The present invention provides a composite seaweed, including Dunaliella and Chlorella. Seaweed is a rich marine resource with multiple biologically active components. Studies have shown that certain components in seaweed can inhibit the production of rumen methane. The effect of a single seaweed is limited, and a composite seaweed formula is used to improve its methane emission reduction effect. Reducing rumen methane emissions in ruminants is of great significance for environmental protection and sustainable development.
[0022] In a specific embodiment of the present invention, the ratio of the Dunaliella to the Chlorella is 1: 1. The carotene content of the Dunaliella is greater than or equal to 3%; the protein content of the Chlorella is greater than or equal to 57.5%.
[0023] In a specific embodiment of the present invention, the composite seaweed is used as a ruminant feed additive. The composite seaweed is added to the basic feed in the form of a wettable powder, emulsion, paste, granule or solution, and the ruminant feed additive is obtained by mixing. The composite seaweed granules are prepared by the following method: obtaining the composite seaweed, desalting, and drying. The composite seaweed is mixed in a certain proportion, and processed by drying, crushing and other processes to make composite seaweed powder. The amount of composite seaweed powder added to ruminant feed can be adjusted according to factors such as the type, weight, and growth stage of the ruminant.
[0024] On the one hand, as a ruminant feed additive, the feed additive is added in an amount of 0.2% to 10% in the feed.
[0025] On the other hand, a method for reducing rumen methane emission from ruminants comprises adding the above feed additive to the feed of the ruminants.
[0026] In a specific embodiment of the present invention, the amount of the feed additive added to the feed is 1% to 5%; specifically, the amount of the feed additive added to the feed is 1.5% or 4.5%.
[0027] In a specific embodiment of the present invention, the feed includes a basic feed and a composite seaweed. The composite seaweed product is in the form of one or more of fresh product, dry product or freeze-dried powder. The composite seaweed is prepared by salt pan cultivation concentration, separation, desalination and drying to obtain fresh seaweed, and the fresh seaweed is processed to obtain dry product powder or freeze-dried powder.
[0028] In a specific embodiment of the present invention, the amount of the composite seaweed added to the feed is based on dry weight and is expressed in weight percentage. The basic feed is selected from feed suitable for ruminants available in the prior art, and the basic feed is selected from corn silage.
[0029] In one specific implementation, composite seaweed is used as a feed additive to reduce gastrointestinal methane production in ruminants.
[0030] In another specific implementation mode, the composite seaweed is used in the preparation of feed with the effect of reducing gastrointestinal methane production in ruminants; the addition amount of the composite seaweed in the feed is 0.2% to 10%. The ruminants include cattle, sheep and deer.
[0031] The composite seaweed is applied to rumen methane reduction in ruminants. The composite seaweed is added to the basic feed. Experimental studies have shown that it has a good gastrointestinal methane reduction effect. The composite seaweed used is Dunaliella and Chlorella. Dunaliella and Chlorella can utilize my country's abundant salt pan aquaculture resources, have mature industrial aquaculture technology, and are easy to obtain. The raw materials used in the present invention are widely available, the cost is low, and the gastrointestinal methane reduction effect is significant. The present invention has important value and significance for my country's animal husbandry methane reduction technology and has great potential for industrial application.
[0032] The instruments, reagents, materials, etc. involved in the following embodiments, unless otherwise specified, are all conventional instruments, reagents, materials, etc. in the prior art and can be obtained through regular commercial channels. The experimental methods, detection methods, etc. involved in the following embodiments, unless otherwise specified, are all conventional experimental methods, detection methods, etc. in the prior art.
[0033] The salt algae and chlorella are mixed in a ratio of 1:1, and processed by drying, crushing and other processes to make composite seaweed powder.
[0034] Example 1: Study on methane emission reduction of Dunaliella and Chlorella In this example, the methane emission reduction effect of Dunaliella and Chlorella was detected by in vitro culture of rumen gastric fluid of adult beef cattle. The specific process is as follows: Gastric juice was obtained from the rumen of 2-year-old yellow cattle (weight 517.5 kg).
[0035] Corn silage was used as the basic feed, and 6.0% salt algae or 6.0% chlorella was added. The seaweed was produced in Tianjin Hangu Salt Field, desalted and dried, and the particle size was 80% over 60 mesh. A total of 3 experimental groups were set up, and 3 parallel tests were set up in each experimental group: 6.0% salt algae group, 6.0% chlorella group, and the basic feed (without compound seaweed) was used as the control group.
[0036] On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, 0.12 g of Dunaliella was added to the 6.0% Dunaliella group, and 0.12 g of Chlorella was added to the 6.0% Chlorella group, and the culture was sealed. The experiment lasted for 1 month (30 days), and the total methane production of each experimental group was detected on the 7th and 30th days, and the average value was taken. The methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine.
[0037] Results: After 7 days, the methane production of the control group was 750.0±36.1 mg, the methane production of the 4.5% Dunaliella group was 108.7±19.4 mg, a decrease of 85.5%, and the methane production of the 1.5% Chlorella group was 116.3±33.2 mg, a decrease of 84.5%.
[0038] After 30 days, the methane production of the control group was 662.5±117.3 mg, the methane production of the 4.5% salt algae group was 388.4±153.4 mg, a decrease of 51.1% (with the passage of culture time, the methane-reducing effective components of salt algae were exhausted and the methane production gradually increased), and the methane production of the 1.5% Chlorella group was 333.2±9.1 mg, a decrease of 49.7%.
[0039] Example 2: Study on methane emission reduction by using Dunaliella and Chlorella In this embodiment, the methane emission reduction effect of the composite algae powder was detected by in vitro culture of the rumen gastric fluid of adult beef cattle. The specific process is as follows: Gastric juice was collected from the rumen of 2-year-old yellow cattle (weight 530.5 kg).
[0040] Corn silage (obtained from Weihai livestock farm) was used as the basic feed, and compound freeze-dried powder was added. Two experimental groups were set up, and two parallel tests were set up in each experimental group: 6.0% compound algae powder group (4.5% Dunaliella + 4.5% Chlorella), and the basic feed (without compound seaweed) was used as the control group.
[0041] Preparation of compound algae powder collected from Tianjin Hangu Salt Field on September 8, 2024 On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, and 0.12 g of composite seaweed was added to the 6.0% composite algae powder group, and the culture was sealed. The experiment lasted for 1 month (28 days), and the total methane production of each experimental group was detected on the 7th and 28th days, and the average value was taken (the methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine).
[0042] Results: After 7 days, the methane production of the control group was 750.0±36.1mgmg, and the methane production of the 6.0% composite algae powder group was 73.5±15.1mg, a decrease of 90.2%.
[0043] After 30 days, the methane production of the control group was 662.5±12.3 mg, and the methane production of the 6.0% composite algae powder group was 295.5±10.5 mg, a decrease of 55.4%.
[0044] Conclusion: Due to the combined effects of Dunaliella and Chlorella, the methane emission reduction effect of composite seaweed is more significant than that of single seaweed. By comparing the effects of different seaweed ratios, it can be seen that the specific composite algae (4.5% Dunaliella, 4.5% Chlorella, and freeze-dried algae powder) has the best experimental effect (the effect is 5.2% higher after 7 days, but 5.4% higher after 30 days), which is significantly better than single seaweed.
[0045] Example 3: Study on methane emission reduction by using Dunaliella and Chlorella In this embodiment, the methane emission reduction effect of the composite algae powder was tested by in vitro culture of adult sheep rumen gastric fluid. The specific process is as follows: Gastric juice was obtained from the rumen of 2-year-old goats (weight 40.5 kg).
[0046] Corn silage was used as the basic feed, and 6.0% salt algae or 6.0% chlorella was added. The seaweed was produced in Tianjin Hangu Salt Field, desalted and dried, and the particle size was 80% over 60 mesh. A total of 3 experimental groups were set up, and 3 parallel tests were set up in each experimental group: 6.0% salt algae group, 6.0% chlorella group, and the basic feed (without compound seaweed) was used as the control group.
[0047] On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, 0.12 g of Dunaliella was added to the 6.0% Dunaliella group, and 0.12 g of Chlorella was added to the 6.0% Chlorella group, and the culture was sealed. The experiment lasted for 1 month (30 days), and the total methane production of each experimental group was detected on the 7th and 30th days, and the average value was taken. The methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine.
[0048] Results: After 7 days, the methane production of the control group was 750.0±36.1 mg, the methane production of the 4.5% Dunaliella group was 108.7±19.4 mg, a decrease of 85.5%, and the methane production of the 1.5% Chlorella group was 116.3±33.2 mg, a decrease of 84.5%.
[0049] After 30 days, the methane production of the control group was 662.5±117.3 mg, the methane production of the 4.5% salt algae group was 388.4±153.4 mg, a decrease of 51.1% (with the passage of culture time, the methane-reducing effective components of salt algae were exhausted and the methane production gradually increased), and the methane production of the 1.5% Chlorella group was 333.2±9.1 mg, a decrease of 49.7%.
[0050] Example 4: Study on methane emission reduction by Dunaliella and Chlorella In this embodiment, the methane emission reduction effect of the composite algae powder was detected by in vitro culture of adult sheep rumen gastric fluid, and the specific process is as follows: Gastric juice was obtained from the rumen of 2-year-old goats (weight 40.5 kg).
[0051] Corn silage (obtained from Weihai livestock farm) was used as the basic feed, and compound freeze-dried powder was added. Two experimental groups were set up, and two parallel tests were set up in each experimental group: 6.0% compound algae powder group (4.5% Dunaliella + 4.5% Chlorella), and the basic feed (without compound seaweed) was used as the control group.
[0052] Preparation of compound algae powder collected from Tianjin Hangu Salt Field on September 8, 2024 On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, and 0.12 g of composite seaweed was added to the 6.0% composite algae powder group, and the culture was sealed. The experiment lasted for 1 month (28 days), and the total methane production of each experimental group was detected on the 7th and 28th days, and the average value was taken (the methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine).
[0053] Results: After 7 days, the methane production of the control group was 750.0±36.1mgmg, and the methane production of the 6.0% composite algae powder group was 73.5±15.1mg, a decrease of 90.2%.
[0054] After 30 days, the methane production of the control group was 662.5±12.3 mg, and the methane production of the 6.0% composite algae powder group was 295.5±10.5 mg, a decrease of 55.4%.
[0055] Conclusion: Due to the combined effects of Dunaliella and Chlorella, the methane emission reduction effect of composite seaweed is more significant than that of single seaweed. By comparing the effects of different seaweed ratios, it can be seen that the specific composite algae (4.5% Dunaliella, 4.5% Chlorella, and freeze-dried algae powder) has the best experimental effect (the effect is 5.2% higher after 7 days, but 5.4% higher after 30 days), which is significantly better than single seaweed.
[0056] Example 5: Study on methane emission reduction by Dunaliella and Chlorella In this embodiment, the methane emission reduction effect of the composite algae powder was tested by in vitro culture of adult deer rumen gastric fluid, and the specific process is as follows: Gastric juice was obtained from the rumen of 2-year-old sika deer (weight 40.5 kg).
[0057] Corn silage was used as the basic feed, and 6.0% salt algae or 6.0% chlorella was added. The seaweed was produced in Tianjin Hangu Salt Field, desalted and dried, and the particle size was 80% over 60 mesh. A total of 3 experimental groups were set up, and 3 parallel tests were set up in each experimental group: 6.0% salt algae group, 6.0% chlorella group, and the basic feed (without compound seaweed) was used as the control group.
[0058] On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, 0.12 g of Dunaliella was added to the 6.0% Dunaliella group, and 0.12 g of Chlorella was added to the 6.0% Chlorella group, and the culture was sealed. The experiment lasted for 1 month (30 days), and the total methane production of each experimental group was detected on the 7th and 30th days, and the average value was taken. The methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine.
[0059] Results: After 7 days, the methane production of the control group was 750.0±36.1 mg, the methane production of the 4.5% Dunaliella group was 108.7±19.4 mg, a decrease of 85.5%, and the methane production of the 1.5% Chlorella group was 116.3±33.2 mg, a decrease of 84.5%.
[0060] After 30 days, the methane production of the control group was 662.5±117.3 mg, the methane production of the 4.5% salt algae group was 388.4±153.4 mg, a decrease of 51.1% (with the passage of culture time, the methane-reducing effective components of salt algae were exhausted and the methane production gradually increased), and the methane production of the 1.5% Chlorella group was 333.2±9.1 mg, a decrease of 49.7%.
[0061] Example 6: Study on methane emission reduction by Dunaliella and Chlorella In this example, the methane emission reduction effect of the composite algae powder was tested by in vitro culture of adult deer rumen gastric fluid, and the specific process is as follows: Gastric juice was obtained from the rumen of 2-year-old sika deer (weight 40.5 kg).
[0062] Corn silage (obtained from Weihai livestock farm) was used as the basic feed, and compound freeze-dried powder was added. Two experimental groups were set up, and two parallel tests were set up in each experimental group: 6.0% compound algae powder group (4.5% Dunaliella + 4.5% Chlorella), and the basic feed (without compound seaweed) was used as the control group.
[0063] Preparation of compound algae powder collected from Tianjin Hangu Salt Field on September 8, 2024 On the first day of the experiment, 2 g of basal feed was added to 200 ml of gastric juice, and 0.12 g of composite seaweed was added to the 6.0% composite algae powder group, and the culture was sealed. The experiment lasted for 1 month (28 days), and the total methane production of each experimental group was detected on the 7th and 28th days, and the average value was taken (the methane content of the gas was measured using a gas chromatograph equipped with an FID detector (GC-FID, PEAK, USA), and the methane content in the gas was detected on the machine).
[0064] Results: After 7 days, the methane production of the control group was 750.0±36.1mgmg, and the methane production of the 6.0% composite algae powder group was 73.5±15.1mg, a decrease of 90.2%.
[0065] After 30 days, the methane production of the control group was 662.5±12.3 mg, and the methane production of the 6.0% composite algae powder group was 295.5±10.5 mg, a decrease of 55.4%.
[0066] Conclusion: Due to the combined effects of Dunaliella and Chlorella, the methane emission reduction effect of composite seaweed is more significant than that of single seaweed. By comparing the effects of different seaweed ratios, it can be seen that the specific composite algae (4.5% Dunaliella, 4.5% Chlorella, and freeze-dried algae powder) has the best experimental effect (the effect is 5.2% higher after 7 days, but 5.4% higher after 30 days), which is significantly better than single seaweed.
[0067] The above description is only an embodiment of the present application and is not intended to limit the protection scope of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A composite seaweed, characterized in that: Includes Dunaliella and Chlorella.
2. The composite seaweed according to claim 1, characterized in that: The ratio of the salt algae to the chlorella is 1:
1.
3. A ruminant feed additive, characterized in that: Comprising the composite seaweed according to claim 1 or 2.
4. The ruminant feed additive according to claim 3, characterized in that: The feed additive is added in an amount of 0.2% to 10% in the feed.
5. A method for reducing rumen methane emissions from ruminants, characterized in that: The method comprises adding the feed additive according to claim 4 to feed for ruminants.
6. The method for reducing rumen methane emission of ruminants according to claim 5, characterized in that: The feed additive is added in an amount of 1% to 5% in the feed.
7. The method for reducing rumen methane emission of ruminants according to claim 5, characterized in that: The feed comprises basic feed and compound seaweed.
8. The method for reducing rumen methane emission of ruminants according to claim 7, characterized in that: The amount of the composite seaweed added to the feed is calculated on a dry basis in the form of weight percentage, and the basic feed is selected from corn silage.
9. Use of the composite seaweed according to claim 1 or 2 as a feed additive for reducing gastrointestinal methane production in ruminants.
10. Use of the composite seaweed according to claim 1 or 2 in preparing feed having the effect of reducing methane production in the gastrointestinal tract of ruminants.