Preparation method and application of cosmetic composition capable of resisting oxidation and brightening skin color

Abstract

The invention relates to the technical field of cosmetic preparation, in particular to a preparation method and application of a cosmetic composition capable of resisting oxidation and brightening skin color, and the preparation method of the composition comprises the following steps: S100, raw material pretreatment, S200, active ingredient extraction and preliminary mixing, S300, liposome / microcapsule preparation, S400, final mixing and blending, S500, packaging, S600, equipment calibration and maintenance, and S700, environment control. S800, preparation before quality detection, S900, quality detection and S1000, reworking treatment and finished product packaging. Through the measures of proportioning the active components, adopting nitrogen protection, adding an antioxidant and the like, the oxidation resistance of the cosmetic is remarkably improved, the active components are effectively prevented from being oxidized in the preparation and storage processes, the high quality of the product is ensured, and the cosmetic is suitable for being used in the field of cosmetics. Through strict control of each link in the preparation process, including but not limited to equipment calibration and maintenance, environment control and quality detection, it is ensured that each performance index of the final product meets the standard.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic preparation, and in particular to a method for preparing an antioxidant and skin-lightening cosmetic composition and an application thereof. Background Art

[0002] In the cosmetics industry, antioxidant and skin-lightening products have always been popular among consumers. However, traditional cosmetics have many shortcomings in the preparation and use process, which limit the full play of their antioxidant properties and skin-lightening effects.

[0003] First, traditional cosmetics often ignore the protection of active ingredients during preparation. Antioxidant ingredients such as ascorbic acid (vitamin C) and tocopherol (vitamin E), although they have good antioxidant properties, are easily affected by factors such as light, heat and oxygen, causing oxidation and deterioration, thus losing their activity. Due to the lack of effective protection of these easily oxidized ingredients in traditional preparation processes, the content of active ingredients in cosmetics is unstable, and the antioxidant properties are greatly reduced.

[0004] Secondly, the environmental control of traditional cosmetics during the preparation process is not strict enough. Factors such as humidity, temperature and cleanliness of the preparation environment have an important impact on the quality and stability of cosmetics. However, some cosmetics manufacturers often ignore the control of these factors during the preparation process, which makes the cosmetics easily contaminated and the ingredients are not mixed evenly, affecting the use effect and stability of the product.

[0005] In addition, the traditional packaging method of cosmetics also has certain defects. Some cosmetics fail to adopt effective sealing measures after filling, which causes oxygen and moisture in the air to easily enter the packaging container and react with the ingredients in the cosmetics, thereby accelerating the deterioration of the product. This not only shortens the shelf life of the cosmetics, but also reduces the product's use effect.

[0006] In summary, traditional cosmetics have many deficiencies in preparation and packaging, which limit the full play of their antioxidant properties and skin-lightening effects. Therefore, there is an urgent need to develop a new method for preparing an antioxidant and skin-lightening cosmetic composition to solve the problems existing in traditional cosmetics. Summary of the invention

[0007] Technical issues solved

[0008] In view of the above-mentioned shortcomings of the prior art, the present invention provides a method for preparing an antioxidant and skin-lightening cosmetic composition and its application. The present invention significantly improves the antioxidant properties of cosmetics by proportioning active ingredients and adopting nitrogen protection and adding antioxidants, effectively preventing the oxidation of active ingredients during preparation and storage, ensuring the high quality of products, and ensuring that various performance indicators of the final product meet the standards through strict control of each link in the preparation process, including but not limited to equipment calibration and maintenance, environmental control and quality testing, which helps to improve the stability and bioavailability of the product.

[0009] Technical Solution

[0010] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0011] A method for preparing an antioxidant and skin-lightening cosmetic composition, the method comprising the following steps:

[0012] S100, raw material pretreatment:

[0013] The following raw materials are selected: water 45%-55%, ascorbic acid (vitamin C) 12%-18%, butylene glycol (whole) 9%-11%, dipropylene glycol 4.5%-5.5%, 1,3-propylene glycol 4.5%-5.5%, dicaprylyl carbonate 3.2%-3.8%, propylene glycol dicaprylate / dicaprate 2.2%-2.8%, caprylic / capric triglyceride 1.3%-1.7%, polyglycerol-10 laurate 0.9%-1.1%, β-glucan 0.6%-0.9%, 1,2-hexanediol Alcohol is added in several times, each time in appropriate amount, polysorbate-60 0.75%-0.95%, glycerol 0.65%-0.85%, polydimethylsiloxane 0.65%-0.85%, glyceryl stearate SE 0.45%-0.55%, glutathione 0.32%-0.38%, panthenol 0.09%-0.11%, polyvinyl pyrrolidone 0.09%-0.11%, xanthan gum 0.09%-0.11%, sunflower seed oil 0.09%-0.11%, carrot root extract in appropriate amount, Hu Radish seed oil, β-carotene 0.009%-0.011%, tocopherol (vitamin E) 0.007%-0.009%, mung bean seed extract, Asian birch bark extract, Rumex crispus root extract, sodium hyaluronate 0.09%-0.11%, ethylhexylglycerin 0.005%-0.007%, grapefruit fruit extract, (daily use) flavor 0.055%-0.065%, gluconolactone 0.045%-0.055%, sodium gluconate 0.04 5%-0.055%, Pullulan polysaccharide 0.045%-0.055%, Guar Hydroxypropyltrimonium Chloride 0.045%-0.055%, Cyclopentasiloxane 0.045%-0.055%, Dimethicone / Vinyl Dimethicone Crosspolymer 0.045%-0.055%, Ethylhexyl Salicylate 0.045%-0.055%, Phytic Acid 0.045%-0.055%, Disodium EDTA 0.018%-0.022%;

[0014] The solid raw materials are crushed by a crushing device to control the particle size within the range of 50-100 microns to ensure uniformity. The liquid raw materials are filtered through a 0.2-0.45 micron filter membrane to remove possible impurities. For moisture-sensitive raw materials including but not limited to ascorbic acid, glutathione and tocopherol, they are immediately placed in a sealed container filled with dry inert gas for storage after crushing and filtering until entering the subsequent preparation steps to prevent the raw materials from being damp and deteriorating. Ascorbic acid has the effects of whitening, lightening, anti-wrinkle, and anti-photodamage in skin care, but because it is unstable and easily oxidized, it needs special storage to ensure that its active ingredients are not destroyed;

[0015] S200, active ingredient extraction and preliminary mixing:

[0016] A solvent system composed of ethanol and water in a ratio of 3:2 is prepared, the pretreated raw material is placed in the solvent system, the overall temperature of the solvent system is stirred at 0-5°C, and the stirring speed is 200-300 rpm for 30-40 minutes to fully dissolve the active ingredient, and then the mixed solution is centrifuged for 10-15 minutes using a centrifuge with a rotation speed of 3000-3500 rpm to remove undissolved impurities to obtain a pure active ingredient mixed solution, wherein the active ingredient contains easily oxidizable ingredients including but not limited to ascorbic acid and tocopherol, in addition to using the ethanol-water solvent system, an appropriate amount of antioxidant including but not limited to sodium vitamin C is added, and the added amount is 0.1%-0.3% of the total mass of the active ingredient, and it is added simultaneously during the stirring process to further prevent oxidation of the active ingredient and ensure its chemical stability;

[0017] S300, liposome / microcapsule preparation:

[0018] Select lipid material in a ratio of phospholipid to cholesterol of 2:1, use a rotary evaporator at a speed of 100-120 rpm and 40-45° C. to rotary evaporate the lipid material for 20-25 minutes to form a uniform film, then add the prepared active ingredient mixture under nitrogen protection, ultrasonically vibrate at a frequency of 30-35 kHz and a pulse interval of 2-3 seconds for 8-12 minutes, and then pass through a polycarbonate membrane with a pore size of 100-150 nanometers and perform high-pressure homogenization at a pressure of 600-700 bar for 3-4 times, and cool to 30-32° C. after each cycle to form liposomes or microcapsules encapsulating the active ingredients;

[0019] S400, final mixing:

[0020] The prepared liposomes or microcapsules are mixed with butylene glycol and propylene glycol solvents, emulsifiers such as Tween-80 and Span-60, hydroxyethyl cellulose, carbomer and other thickeners, and a new cross-linking agent, and stirred at a speed of 350-400 rpm for 15-20 minutes, and then the stirring speed is gradually reduced to 100-150 rpm within 15-20 minutes, and the stirring is continued for 30-40 minutes to fully mix the ingredients;

[0021] S500, Packaging:

[0022] The packaging containers such as glass bottles or plastic bottles are sterilized with high-temperature steam at a temperature of 121°C for 15-20 minutes. In a clean workshop with a cleanliness level of 10,000, the mixed cosmetic composition is filled into the container at a rate of 5-10 ml per second. After filling, nitrogen is immediately used for sealing to ensure the sealing and stability of the packaging.

[0023] Furthermore, the method for preparing the composition further comprises the following steps:

[0024] S600, Equipment Calibration and Maintenance:

[0025] The agitator, rotary evaporator and high-pressure homogenizer are calibrated every 1-2 months. The calibration content includes but is not limited to rotation speed, temperature and pressure. Standard measuring instruments are used for testing to ensure that the parameter error is within ±5%. At the same time, the equipment is cleaned every week and key components of the equipment are replaced every 3-6 months, including but not limited to stirring paddles, evaporating flasks and homogenizing valves. Before using the equipment each time, run it idle for 5-10 minutes to check whether the equipment is operating normally. The use parameters and maintenance status of the equipment are recorded each time, including but not limited to calibration time, maintenance items and parts replacement information, so as to facilitate quality traceability and process improvement.

[0026] Furthermore, the method for preparing the composition further comprises the following steps:

[0027] S700, Environmental Control:

[0028] During the entire preparation process, the humidity of the preparation environment is controlled at 45%-55%, and the temperature is controlled at 20-23°C. Solid raw materials are stored in a special dry, light-proof warehouse. The warehouse temperature is maintained at 18-20°C and the relative humidity is lower than 40%. Liquid raw materials are stored in a cool place with a temperature not exceeding 25°C. By installing air conditioning and dehumidification equipment, environmental parameters are monitored and adjusted in real time to ensure that the raw materials and the preparation process are not affected by environmental factors;

[0029] S800, Preparation before quality inspection:

[0030] After the final mixing and preparation, the mixed cosmetic composition is subjected to a preliminary standing treatment for 12-24 hours to further evenly distribute the ingredients therein and eliminate possible bubbles and stratification.

[0031] Furthermore, the method for preparing the composition further comprises the following steps:

[0032] S900, quality inspection:

[0033] The content of active ingredients in the cosmetic composition is determined by high performance liquid chromatography according to specific detection conditions including but not limited to mobile phase composition, flow rate and column temperature to ensure that the content of each active ingredient is within the set range. The particle size distribution of liposomes or microcapsules is detected by dynamic light scattering, requiring that the particle size is mainly concentrated in the range of 100-200 nanometers and the distribution uniformity meets certain standards. The plate count method is used to detect microbial limits according to national standards. The total number of colonies, molds and yeasts per gram of cosmetics should be lower than the specified limit. The pH value of the cosmetic composition is measured using a pH meter, and the pH value is between 4 and 7;

[0034] S1000, rework and finished product packaging:

[0035] Based on the quality test results, batches that do not meet the standards for active ingredient content, particle size distribution, microbial limits or pH value will be reworked. The rework process includes re-extraction of active ingredients, re-preparation of liposomes / microcapsules or mixing and blending until all test indicators are qualified. Only products that pass all test items can be packaged for market sale. The product name, ingredients, shelf life and production batch information should be marked on the packaging.

[0036] Furthermore, in the S200 active ingredient extraction and preliminary mixing step, the active ingredient contains easily oxidized ingredients including but not limited to ascorbic acid and tocopherol. In addition to adding sodium vitamin C as an antioxidant, inert gas nitrogen is also introduced to protect the mixed solution. The introduction rate of the inert gas is controlled at 0.1-0.3 L / min, and it is continuously introduced throughout the entire process from the start of stirring to the full dissolution of the active ingredient and the completion of centrifugal treatment, so as to better prevent the oxidation of the active ingredient and ensure its chemical stability.

[0037] Furthermore, in the S300 liposome / microcapsule preparation step, when the high-pressure homogenization method is used, the homogenization pressure and the number of cycles are adjusted according to the characteristics of the active ingredient and the required liposome or microcapsule performance. Specifically, the homogenization pressure is increased to 700-800 bar and the number of cycles is increased to 4-6 times to ensure that the active ingredient can be completely encapsulated in the liposome or microcapsule and has good release characteristics. During the high-pressure homogenization process, the sealing components of the homogenization equipment are inspected and maintained every 1-2 cycles to ensure that the equipment is well sealed and prevent leakage of active ingredients.

[0038] Furthermore, in the final mixing and preparation step S400, the consistency of the cosmetic composition needs to be adjusted, and different types and amounts of thickeners need to be added, including but not limited to adding an appropriate amount of guar gum when a higher consistency is required, and the added amount is 0.5%-1% of the total mixed material. It is slowly added and fully mixed during the stirring process to achieve the required consistency requirements without affecting the performance of other ingredients and the release characteristics of the active ingredients. After adding the thickener, continue stirring for 10-15 minutes to ensure that the thickener is fully integrated with the other ingredients to form a uniform mixture.

[0039] Furthermore, in the S500 packaging step, for the packaging of export products, in addition to the conventional sealing method, an additional layer of moisture-proof and oxygen-proof aluminum foil packaging bag is wrapped outside the packaging container and heat-sealed to ensure that the product can effectively prevent the external environment from affecting the product quality during transportation and storage, and ensure the stability and effectiveness of the product. At the same time, before packaging, the packaging container and aluminum foil packaging bag are sterilized with ultraviolet light for 5-10 minutes, and the ultraviolet light intensity is controlled at 1000-1500μW / cm 2 , to further reduce the risk of product contamination by microorganisms.

[0040] Beneficial Effects

[0041] Compared with the known public technology, the technical solution provided by the present invention has the following beneficial effects:

[0042] 1. The method for preparing the antioxidant and skin-lightening cosmetic composition of the present invention selects a variety of active ingredients with antioxidant functions, including but not limited to ascorbic acid (vitamin C), tocopherol (vitamin E) and glutathione, and adopts liposome / microcapsule encapsulation technology to effectively protect these easily oxidized active ingredients and improve their stability. At the same time, by optimizing the preparation process, such as controlling the humidity and temperature of the preparation environment, pre-treating and finely blending the raw materials, it is ensured that the various ingredients can be fully mixed and exert a synergistic effect.

[0043] 2. The preparation method described in the present invention also focuses on improving the stability of cosmetics. Through strict control of each link in the preparation process, including but not limited to equipment calibration and maintenance, environmental control and quality inspection, it is ensured that the various performance indicators of the final product meet the standards. In particular, in the liposome / microcapsule preparation process, a high-pressure homogenization method is used and the homogenization pressure and the number of cycles are adjusted to make the particle size distribution of the liposome or microcapsule more uniform and mainly concentrated in the appropriate nanometer range, which helps to improve the stability and bioavailability of the product. At the same time, the present invention further extends the shelf life of the product by adding an appropriate amount of antioxidants and using inert gas protection and other measures. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] The present invention will be further described below in conjunction with the embodiments.

[0046] Example 1

[0047] Raw material selection:

[0048] Select the raw materials according to the following proportions: water 50%, ascorbic acid (vitamin C) 15%, butylene glycol 10%, dipropylene glycol 5%, 1,3-propylene glycol 5%, dicaprylyl carbonate 3.5%, propylene glycol dicaprylate / dicaprate 2.5%, caprylic / capric triglyceride 1.5%, polyglyceryl-10 laurate 1%, β-glucan 0.75%, 1,2-hexanediol (add in several times), polysorbate-60 0.85%, glycerol 0.75%, polydimethylsiloxane 0.75%, glyceryl stearate SE 0.5%, glutathione 0.35%, panthenol 0.1%, polyvinyl pyrrolidone 0.1%, xanthan gum 0.1%, sunflower seed oil 0.1%, carrot root extract (add in several times), carrot Appropriate amount of radish seed oil, β-carotene 0.01%, tocopherol (vitamin E) 0.008%, appropriate amount of mung bean seed extract, appropriate amount of Asian birch bark extract, appropriate amount of Rumex crispus root extract, appropriate amount of sodium hyaluronate 0.1%, ethylhexylglycerin 0.006%, appropriate amount of grapefruit fruit extract, (daily use) flavor 0.06%, gluconolactone 0.05%, sodium gluconate 0.05%, budding pullulan polysaccharide 0.05%, guar hydroxypropyltrimonium chloride 0.05%, cyclopentasiloxane 0.05%, polydimethylsiloxane / vinyl polydimethylsiloxane crosspolymer 0.05%, ethylhexyl salicylate 0.05%, inositol hexaphosphate 0.05%, disodium EDTA 0.02%.

[0049] Raw material pretreatment:

[0050] Solid raw material processing: Use crushing equipment to crush ascorbic acid, glutathione, β-carotene, polyvinyl pyrrolidone, xanthan gum, guar hydroxypropyltrimethylammonium chloride and other solid raw materials to control the particle size within the range of 50-100 microns to ensure uniformity. This step is particularly important for ascorbic acid, because ascorbic acid has multiple functions in skin care, such as whitening, anti-wrinkle, and anti-photodamage, but it is unstable itself. Smaller particle size helps it to fully dissolve and evenly disperse in the solvent system later, while reducing the risk of oxidation caused by excessive local concentration.

[0051] Processing of liquid raw materials: butylene glycol, dipropylene glycol, 1,3-propanediol, dicaprylyl carbonate, propylene glycol dicaprylate / dicaprate, caprylic / capric triglyceride, polyglyceryl-10 laurate, 1,2-hexanediol, polysorbate-60, glycerin, dimethicone, glyceryl stearate SE, panthenol, sunflower seed oil, ethylhexylglycerin, grapefruit fruit extract, flavor, gluconolactone, sodium gluconate, budding pullulanase polysaccharide, cyclopentasiloxane, dimethicone / vinyl dimethicone crosspolymer, ethylhexyl salicylate, phytic acid, disodium EDTA and other liquid raw materials are filtered through a 0.2-0.45 micron filter membrane to remove possible impurities. Pure liquid raw materials help to ensure the quality and stability of the entire cosmetic composition and avoid the influence of impurities on active ingredients and possible chemical reactions.

[0052] Storage of moisture-sensitive raw materials: For moisture-sensitive raw materials such as ascorbic acid, glutathione and tocopherol, after crushing and filtering, they should be immediately placed in a sealed container filled with dry nitrogen (as an inert gas) for storage until entering the subsequent preparation steps to prevent the raw materials from getting damp and deteriorating. The stability of ascorbic acid is crucial to its efficacy in cosmetics. Moisture may accelerate its oxidation process and reduce its antioxidant and skin-brightening effects.

[0053] Active ingredient extraction and preliminary mixing:

[0054] Solvent system configuration: A solvent system consisting of ethanol and water in a ratio of 3:2 is configured. This ratio of the solvent system can provide a certain polar environment while ensuring the solubility of the active ingredient, which is beneficial to the interaction between the subsequent antioxidant and the active ingredient, and helps maintain the stability of the system in a low temperature environment and reduce the oxidation risk of the active ingredient.

[0055] Raw material dissolution and stirring: the pretreated raw material is placed in the solvent system, and the overall temperature of the solvent system is stirred at 0-5°C, and stirred at a stirring speed of 250 rpm for 35 minutes to fully dissolve the active ingredient. In this process, in addition to using the ethanol-water solvent system, sodium vitamin C is added as an antioxidant, and the addition amount is 0.2% of the total mass of the active ingredient. At the same time, nitrogen is introduced to protect the mixed liquid. The nitrogen introduction speed is controlled at 0.2 liters / minute, and it is continuously introduced from the beginning of stirring to the full dissolution of the active ingredient and the completion of centrifugal treatment. The low temperature environment can effectively reduce the chemical reaction rate and reduce the possibility of oxidation of the active ingredient during the dissolution process; the appropriate stirring speed can not only promote the dissolution of the raw material, but also avoid accelerating oxidation due to the introduction of too much air due to too fast stirring. Sodium vitamin C as an antioxidant can capture free radicals in the system and further protect easily oxidized components such as ascorbic acid. The continuous introduction of nitrogen forms an inert gas layer above the liquid surface to isolate oxygen and provide a stable dissolution environment for the active ingredient.

[0056] Centrifugation: The mixture was centrifuged at 3500 rpm for 12 minutes to remove undissolved impurities and obtain a pure active ingredient mixture. High-speed centrifugation can use centrifugal force to quickly precipitate and separate insoluble impurities, ensuring the purity of the active ingredient mixture and providing high-quality raw materials for subsequent liposome / microcapsule preparation, avoiding the influence of impurities on the structure and performance of liposomes / microcapsules, thereby ensuring that the active ingredients can be effectively encapsulated and stably present.

[0057] Liposome / microcapsule preparation:

[0058] Lipid material treatment: lipid material was selected at a ratio of phospholipid to cholesterol of 2:1, and the lipid material was rotary evaporated at 42° C. at a speed of 110 rpm for 22 minutes to form a uniform film.

[0059] Addition of active ingredients and ultrasonic oscillation: The prepared active ingredient mixture was added under nitrogen protection, and ultrasonic oscillation was performed for 10 minutes at a frequency of 32kHz and a pulse interval of 2.5 seconds. Nitrogen protection continued to maintain the low oxygen environment of the system to prevent the active ingredients from being oxidized during the addition process; ultrasonic oscillation utilized the cavitation effect of ultrasound to evenly disperse the active ingredient mixture in the lipid film, promote the interaction between the lipid material and the active ingredient, form a preliminary encapsulation structure, and avoid excessive energy input to damage the active ingredients.

[0060] High-pressure homogenization treatment: Then pass through a polycarbonate membrane with a pore size of 120 nanometers and perform high-pressure homogenization at a pressure of 650 bar for 4 times. Cool to 31°C after each cycle to form liposomes or microcapsules that encapsulate active ingredients. During the high-pressure homogenization process, the sealing components of the homogenization equipment are inspected and maintained every other cycle to ensure that the equipment is well sealed and prevent leakage of active ingredients. The high-pressure homogenization process can further refine the particle size of the liposomes / microcapsules so that they are mainly concentrated in the range of 100-200 nanometers. This size range is conducive to the liposomes / microcapsules penetrating the stratum corneum of the skin and improving the percutaneous absorption of active ingredients such as ascorbic acid, thereby better exerting their efficacy in skin care. The cooling step after each cycle helps to stabilize the structure of the liposomes / microcapsules and prevent denaturation of lipid materials or inactivation of active ingredients due to excessive temperature. Regular inspection of the sealing components can ensure that the high-pressure homogenization process is carried out in a closed environment to avoid losses and potential safety risks caused by leakage of active ingredients.

[0061] Final Mix:

[0062] Mixing and preliminary stirring of ingredients: Mix the prepared liposomes or microcapsules with butylene glycol and propylene glycol solvents, Tween-80 and Span-60 emulsifiers, hydroxyethyl cellulose, carbomer and other thickeners, and a new cross-linking agent, and stir at 380 rpm for 18 minutes.

[0063] Adjustment of stirring speed and continuous stirring: Then gradually reduce the stirring speed to 120 rpm within 18 minutes, and continue stirring for 35 minutes to fully mix the ingredients. Gradually reducing the stirring speed can reduce bubbles caused by excessive stirring and make the system more stable and uniform. Continue stirring for a sufficient time to ensure that the ingredients fully interact with each other to form a stable emulsion or gel structure, ensure the uniformity and stability of the cosmetics during use, and enable the active ingredients to be evenly distributed and continue to play a role. During this process, if it is necessary to adjust the consistency of the cosmetic composition, for example, when a higher consistency is required, an appropriate amount of guar gum can be added, the amount of which is 0.8% of the total mixed material, and it is slowly added and fully mixed during the stirring process. After adding the thickener, continue stirring for 12 minutes to ensure that the thickener is fully integrated with other ingredients to form a uniform mixture. As a commonly used thickener, guar gum has been optimized in terms of its addition amount and addition method, which can achieve the purpose of adjusting the consistency without affecting the performance of other ingredients and the release characteristics of active ingredients.

[0064] Package:

[0065] Sterilization of packaging containers: The glass bottle packaging containers are sterilized with high-temperature steam, where the temperature is 121°C and the sterilization time is 18 minutes.

[0066] Filling and sealing: In a cleanroom with a cleanliness level of 10,000, the mixed cosmetic composition is filled into the container at a speed of 8 ml per second. After filling, it is immediately sealed with nitrogen. The cleanroom environment can minimize the contamination of dust particles and microorganisms in the air to the product and ensure the purity of the product. The specific filling speed is determined through experiments, which can not only ensure production efficiency but also avoid excessive bubbles or splashes during the filling process. After filling, it is immediately sealed with nitrogen. The inertness of nitrogen is used to exclude the air in the container, further preventing the cosmetic composition from oxidizing and deteriorating due to contact with oxygen during storage and use, protecting the stability of active ingredients such as ascorbic acid, and ensuring that the product continues to maintain good antioxidant and skin-brightening effects during its shelf life.

[0067] Equipment calibration and maintenance:

[0068] Regular calibration: The agitator, rotary evaporator and high-pressure homogenizer are calibrated every 1.5 months. The calibration content includes speed, temperature and pressure. Standard measuring tools are used for testing to ensure that the parameter error is within ±5%.

[0069] Daily cleaning and replacement of key parts: Clean the equipment weekly and replace key parts of the equipment every 4 months, such as stirring blades, evaporation flasks and homogenizing valves. Before using the equipment each time, run it idle for 8 minutes to check whether it is operating normally, and record the use parameters and maintenance of the equipment each time, including calibration time, maintenance items and parts replacement information, so as to facilitate quality traceability and process improvement.

[0070] Environmental Control:

[0071] Preparation environment control: During the entire preparation process, the humidity of the preparation environment is controlled at 50% and the temperature is controlled at 22°C by installing air conditioners and dehumidification equipment. Excessive humidity may cause the raw materials to become damp and deteriorate, especially for humidity-sensitive raw materials such as ascorbic acid (combined with the properties of ascorbic acid), which will accelerate its oxidation process and reduce the effectiveness of the active ingredients. Too high or too low temperature may affect the chemical reaction rate, the physical state of the raw materials and the operating performance of the equipment. For example, in the active ingredient extraction and preliminary mixing steps, a low temperature environment helps to reduce the oxidation of the active ingredients, but if the temperature is too low, it may cause some raw materials to solidify or increase viscosity, affecting the stirring and dissolution effects. Therefore, controlling the ambient temperature at 22°C can provide relatively stable conditions for each preparation step, ensuring the normal progress of the reaction, the stability of the raw material performance and the normal operation of the equipment, thereby ensuring the consistency of product quality.

[0072] Raw material storage environment control: Solid raw materials are stored in a special dry, light-proof warehouse. The warehouse temperature is maintained at 19°C and the relative humidity is below 40%. Liquid raw materials are stored in a cool place with a temperature not exceeding 25°C to ensure that the raw materials and the preparation process are not affected by environmental factors. The dry, light-proof warehouse environment can effectively prevent the solid raw materials from absorbing moisture, oxidation and light decomposition, and protect the stability and activity of the raw materials. Ascorbic acid is easily oxidized and deteriorated under light and humid conditions. Strict control of the warehouse environment can extend its storage life and ensure that it still has good performance when used.

[0073] Preparation before quality inspection:

[0074] After the final mixing and preparation, the mixed cosmetic composition is subjected to a preliminary standing treatment for 18 hours to allow the ingredients therein to be further evenly distributed and to eliminate possible bubbles and stratification.

[0075] Quality Inspection:

[0076] Determination of active ingredient content: High performance liquid chromatography is used to determine the content of active ingredients in the cosmetic composition according to specific detection conditions (such as mobile phase composition, flow rate and column temperature, etc.) to ensure that the content of each active ingredient is within the set range. Among them, ascorbic acid is a key active ingredient, and the accuracy of its content directly affects the product's antioxidant and skin-lightening effects. High performance liquid chromatography has the characteristics of high separation efficiency, high sensitivity and accuracy, and can accurately determine the content of various active ingredients in cosmetics, including ascorbic acid, tocopherol, glutathione, etc. By comparing and analyzing with standard products, the content of active ingredients is strictly controlled within the specified range to ensure the consistency and stability of product efficacy, so that each batch of products can provide consumers with reliable antioxidant and skin-lightening effects.

[0077] Particle size distribution detection: The particle size distribution of liposomes or microcapsules is detected by dynamic light scattering. The particle size is required to be mainly concentrated in the range of 100-200 nanometers and the distribution uniformity meets certain standards.

[0078] Microbial limit detection: The plate count method is used to detect microbial limits in accordance with national standards. The total number of colonies, molds and yeasts per gram of cosmetics should be lower than the prescribed limits.

[0079] pH measurement: The pH value of the cosmetic composition was measured using a pH meter, and the pH value was between 4-7.

[0080] Rework and finished product packaging:

[0081] According to the quality test results, any batch that does not meet the standards in terms of active ingredient content, particle size distribution, microbial limit or pH value will be reworked. The rework process includes re-extraction of active ingredients, liposome / microcapsule preparation or mixing and blending until all test indicators are qualified. Only products that pass all test items can be packaged for market sale. The product name, ingredients, shelf life and production batch information should be marked on the packaging. For the packaging of export products, in addition to the conventional sealing method, an additional layer of moisture-proof and oxygen-proof aluminum foil packaging bag should be wrapped outside the packaging container and heat-sealed. Before packaging, the packaging container and aluminum foil packaging bag should be sterilized by ultraviolet light for 8 minutes, and the ultraviolet light intensity should be controlled at 1200μW / cm 2 , in order to further reduce the risk of product contamination by microorganisms, ensure that the product can effectively prevent the impact of the external environment on product quality during transportation and storage, and ensure the stability and effectiveness of the product.

[0082] Example 2

[0083] Raw material selection:

[0084] The same raw material selection ratio as in Example 1.

[0085] Raw material pretreatment:

[0086] Solid raw material processing: Same as Example 1, the solid raw material was crushed to 50-100 microns.

[0087] Liquid raw material treatment: Same as Example 1, the liquid raw material is filtered.

[0088] Storage of humidity-sensitive raw materials: As in Example 1, the humidity-sensitive raw materials are placed in a nitrogen-filled sealed container for storage.

[0089] Active ingredient extraction and preliminary mixing:

[0090] Solvent system configuration: Same as in Example 1, except that an ethanol-water solvent system (3:2) was configured.

[0091] Dissolving and stirring raw materials: put the raw materials into the solvent system, keep the overall temperature of the solvent system at 0-5°C and stir at 200 rpm for 40 minutes, add 0.1% of the total mass of active ingredients of sodium vitamin C as an antioxidant, and pass nitrogen protection at a speed of 0.1 L / min until centrifugation is completed.

[0092] Centrifugation: Centrifuge at 3000 rpm for 15 minutes to obtain a pure active ingredient mixture.

[0093] Liposome / microcapsule preparation:

[0094] Lipid material treatment: Select lipid material according to the ratio of phospholipid to cholesterol 2:1, and form a film by rotary evaporation at 100 rpm and 40°C for 25 minutes.

[0095] Addition of active ingredients and ultrasonic oscillation: Add the active ingredient mixture under nitrogen protection and ultrasonically oscillate for 12 minutes at 30 kHz and a pulse interval of 2 seconds.

[0096] High pressure homogenization: through a 100 nm polycarbonate membrane, high pressure homogenization at 700 bar for 6 times (cooling to 30° C. after each cycle), and checking the sealing parts every 2 cycles.

[0097] Final Mix:

[0098] Mixing and initial stirring of ingredients: Mix the liposomes or microcapsules with the solvent, emulsifier, thickener and cross-linking agent, and stir at 350 rpm for 20 minutes.

[0099] Stirring speed adjustment and continuous stirring: reduce to 100 rpm within 15 minutes and continue stirring for 40 minutes. If a higher consistency is required, add 1% guar gum to the total mixture (add slowly and stir for 15 minutes).

[0100] Package:

[0101] Sterilization of packaging containers: Steam sterilize plastic bottle packaging containers at 121°C for 15 minutes.

[0102] Filling and sealing: Filling at 5 ml per second in a clean room, nitrogen sealing, export products are heat-sealed in aluminum foil bags, and containers and aluminum foil bags are sterilized with ultraviolet light for 5 minutes before packaging (intensity 1500μW / cm 2 ).

[0103] Equipment calibration and maintenance:

[0104] Regular calibration: calibrate the agitator, rotary evaporator and high-pressure homogenizer every month to ensure that the parameter error is within ±5%.

[0105] Daily cleaning and replacement of key parts: Clean the equipment weekly, replace key parts every 3 months, run it idle for 5 minutes before each use and record parameters and maintenance status.

[0106] Environmental Control:

[0107] Preparation environment control: The preparation environment humidity was controlled at 45% and the temperature was controlled at 20°C.

[0108] Raw material storage environment control: solid raw materials are stored in a dry, dark warehouse at 18°C ​​and humidity below 40%; liquid raw materials are stored in a cool place (not exceeding 25°C).

[0109] Preparation before quality inspection:

[0110] After mixing, let it sit for 24 hours.

[0111] Quality Inspection:

[0112] Determination of active ingredient content: High performance liquid chromatography was used to measure the active ingredient content.

[0113] Particle size distribution detection: Dynamic light scattering method is used to measure particle size distribution (100-200 nanometers and the uniformity meets the standard).

[0114] Microbial limit test: Plate count method is used to measure microbial limits.

[0115] pH measurement: pH meter is used to measure pH value (4-7).

[0116] Rework and finished product packaging:

[0117] Unqualified batches are reworked according to the corresponding steps until they are qualified, and qualified products are labeled with information and packaged for sale.

[0118] Example 3

[0119] Raw material selection:

[0120] The same raw material selection ratio as in Example 1.

[0121] Raw material pretreatment:

[0122] Solid raw material processing: Same as Example 1, the solid raw material was crushed to 50-100 microns.

[0123] Liquid raw material treatment: Same as Example 1, the liquid raw material is filtered.

[0124] Storage of humidity-sensitive raw materials: As in Example 1, the humidity-sensitive raw materials are placed in a nitrogen-filled sealed container for storage.

[0125] Active ingredient extraction and preliminary mixing

[0126] Solvent system configuration: Same as in Example 1, except that an ethanol-water solvent system (3:2) was configured.

[0127] Dissolving and stirring raw materials: put the raw materials into the solvent system, keep the overall temperature of the solvent system at 0-5°C and stir at 300 rpm for 30 minutes, add 0.3% of the total mass of active ingredients of sodium vitamin C as an antioxidant, and pass nitrogen protection at a speed of 0.3 L / min until centrifugation is completed.

[0128] Centrifugation: Centrifuge at 3500 rpm for 10 minutes to obtain a pure active ingredient mixture.

[0129] Liposome / microcapsule preparation:

[0130] Lipid material treatment: Select lipid material according to the ratio of phospholipid to cholesterol 2:1, and form a film by rotary evaporation at 120 rpm and 45°C for 20 minutes.

[0131] Addition of active ingredients and ultrasonic oscillation: Add the active ingredient mixture under nitrogen protection and ultrasonically oscillate for 8 minutes at 35 kHz and a pulse interval of 3 seconds.

[0132] High pressure homogenization: through a 150 nm polycarbonate membrane, high pressure homogenization at 800 bar for 4 times (cooling to 32° C. after each cycle), and checking the sealing parts every other cycle.

[0133] Final Mix:

[0134] Mixing and preliminary stirring of ingredients: Mix the liposomes or microcapsules with the solvent, emulsifier, thickener and cross-linking agent, and stir at 400 rpm for 15 minutes.

[0135] Adjustment of stirring speed and continuous stirring: Reduce to 150 rpm within 20 minutes and continue stirring for 30 minutes. If a higher consistency is required, add 0.5% guar gum to the total mixture (add slowly and stir for 10 minutes).

[0136] Package:

[0137] Sterilization of packaging containers: Steam sterilize glass bottle packaging containers at 121°C for 20 minutes.

[0138] Filling and sealing: Filling at 10 ml per second in a clean room, nitrogen-filled and sealed, export products are heat-sealed in aluminum foil bags, and containers and aluminum foil bags are sterilized with ultraviolet light for 10 minutes before packaging (intensity 1000μW / cm 2 ).

[0139] Equipment calibration and maintenance:

[0140] Regular calibration: Calibrate the agitator, rotary evaporator and high-pressure homogenizer every 2 months to ensure that the parameter error is within ±5%.

[0141] Daily cleaning and replacement of key parts: Clean the equipment weekly, replace key parts every 6 months, run it idle for 10 minutes before each use and record parameters and maintenance status.

[0142] Environmental Control:

[0143] Preparation environment control: The preparation environment humidity was controlled at 55% and the temperature was controlled at 23°C.

[0144] Raw material storage environment control: solid raw materials are stored in a dry, dark warehouse at 20°C and humidity below 40%; liquid raw materials are stored in a cool place (not exceeding 25°C).

[0145] Preparation before quality inspection:

[0146] After mixing, let it sit for 12 hours.

[0147] Quality Inspection:

[0148] Determination of active ingredient content: High performance liquid chromatography was used to measure the active ingredient content.

[0149] Particle size distribution detection: Dynamic light scattering method is used to measure particle size distribution (100-200 nanometers and the uniformity meets the standard).

[0150] Microbial limit test: Plate count method is used to measure microbial limits.

[0151] pH measurement: pH meter is used to measure pH value (4-7).

[0152] Rework and finished product packaging:

[0153] Unqualified batches are reworked according to the corresponding steps until they are qualified, and qualified products are labeled with information and packaged for sale.

[0154] Test examples and comparison results:

[0155] In order to evaluate the actual effect of the cosmetic composition of the present invention, the following test examples were conducted and compared with a common antioxidant skin-lightening cosmetic (control sample) on the market.

[0156] Test Example 1: Antioxidant Performance Test

[0157] Test method:

[0158] The antioxidant capacity of the cosmetic composition was determined by the DPPH free radical scavenging method. The cosmetic compositions prepared in different embodiments and the comparative samples were respectively prepared into solutions of a certain concentration. An equal volume of the solution was mixed with the DPPH free radical solution. After standing in a dark environment for 30 minutes, the absorbance of the solution at a wavelength of 517 nm was measured.

[0159] Test results:

[0160]

[0161]

[0162] Result analysis:

[0163] The free radical scavenging rate of Example 3 is the highest, indicating that its antioxidant performance is relatively good. This may be because in the active ingredient extraction and preliminary mixing steps of Example 3, the stirring speed is faster and the amount of antioxidant added is relatively large, and a higher high-pressure homogenization pressure is used in the liposome / microcapsule preparation step, so that the active ingredients are better encapsulated and protected, thereby performing better in the antioxidant test. The antioxidant performance of Example 1 is also relatively outstanding, while that of Example 2 is relatively weak, but both are better than the control samples.

[0164] Test Example 2: Evaluation of skin lightening effect

[0165] Test method:

[0166] 30 female volunteers aged between 25 and 45 with dark skin and uneven skin tone were selected and randomly divided into three groups, each group with 10 people. The cosmetic compositions prepared in Example 1, Example 2 and Example 3 were used respectively, and the control sample was used for another group of 10 volunteers. After cleansing their faces in the morning and evening every day, the volunteers took an appropriate amount of the product and evenly applied it on the face for 8 consecutive weeks. Before use, after 4 weeks of use and after 8 weeks of use, a professional skin colorimeter (such as Konica Minolta CM-2600d) was used to measure the L value (indicating the brightness of the skin, the higher the L value, the brighter the skin), a value (indicating the red-green degree of the skin, +a indicates red, -a indicates green) and b value (indicating the yellow-blue degree of the skin, +b indicates yellow, -b indicates blue) of the facial skin of the volunteers, and ΔL (the difference between the L value after use and the L value before use), Δa and Δb were calculated. The skin lightening effect of the product was evaluated by analyzing these data.

[0167] Test results:

[0168]

[0169]

[0170] Result analysis:

[0171] Brightness increase (ΔL):

[0172] After 4 weeks of use, Example 3 had the highest ΔL value, indicating that it had the most obvious effect of brightening skin tone in the short term, followed by Example 1, and Example 2 also had a certain effect, and all were significantly better than the control sample.

[0173] After 8 weeks of use, the skin brightness improvement of Example 3 is still the most significant, and Examples 1 and 2 also maintain a good brightening effect. The brightness improvement of the control sample is relatively limited, which shows that the cosmetic composition of the present invention can continue to play a role in brightening the skin color during long-term use, and the formula and process of Example 3 have certain advantages in promoting skin brightness improvement.

[0174] Skin tone improvement (Δa and Δb):

[0175] As for the Δa value, as the use time increases, each example group and the control sample group increases to a certain extent, indicating that the skin redness is improved to a certain extent, but the increase in the example group is greater, indicating that the product of the present invention has a better effect on improving the skin redness. Among them, Example 3 has the highest Δa value after 8 weeks, indicating that it performs well in improving the skin redness.

[0176] In terms of Δb values, all example groups and control groups were negative and their absolute values ​​increased with increasing usage time, indicating that the yellowness of the skin was decreasing, that is, the skin became fairer. The absolute values ​​of Δb in the example groups were greater than those in the control groups, and the absolute values ​​of Δb in Example 3 were the largest after 4 and 8 weeks, indicating that Example 3 was more effective in reducing the yellowness of the skin and fairing the skin.

[0177] Test Example 3: Stability Test

[0178] Test method:

[0179] High temperature stability test:

[0180] The cosmetic compositions prepared in different embodiments and the control samples were placed in a constant temperature box at 45° C. for 60 days, and the appearance of the samples was observed (such as whether there was stratification, discoloration, precipitation, etc.), and samples were taken before and after placement, and the active ingredient content, particle size distribution and pH value were determined according to the above-mentioned quality inspection method.

[0181] Low temperature stability test:

[0182] The samples were placed in a low temperature environment of -10°C for 30 days, and the appearance changes were observed and related indicators were measured.

[0183] Light stability test:

[0184] The samples were placed in a light box with a light intensity of 5000 lux for 30 days, and the appearance was observed and the indicators were tested.

[0185] Test results:

[0186]

[0187]

[0188] Result analysis:

[0189] High temperature stability:

[0190] The appearance of Examples 1 and 3 did not change significantly at high temperatures, the loss of active ingredient content was relatively small, and the changes in particle size distribution and pH value were also small, indicating that their high temperature stability was good. Example 2 showed slight discoloration, which may be related to the slightly poor stability of some of its components at high temperatures, but the overall performance was still better than the control sample, which showed obvious stratification and precipitation at high temperatures, a large loss of active ingredients, obvious changes in particle size and pH value, and poor stability.

[0191] Low temperature stability:

[0192] The three examples showed no obvious changes or only slight changes at low temperatures, while the control sample became turbid, and the loss of active ingredients and the change in particle size were large, indicating that the cosmetic composition of the present invention has better stability in a low temperature environment, which is better than the control sample.

[0193] Light stability:

[0194] Examples 1 and 3 only slightly changed color under light, Example 2 changed color relatively obviously, and the comparative sample showed serious color change and odor, and the loss of active ingredients and the change of particle size were both large. This shows that the product of the present invention has a certain light stability, among which Example 3 has relatively good light stability, and the comparative sample has the worst light stability.

[0195] Through the comparison of the above test examples, it can be seen that the antioxidant and skin-lightening cosmetic composition prepared by the present invention is superior to ordinary comparative samples on the market in terms of antioxidant performance, skin-lightening effect and stability. There are also certain differences between different embodiments. Embodiment 3 shows better performance in many aspects, followed by Embodiment 1, and Embodiment 2 also has its advantages. In actual production and application, the appropriate embodiment scheme can be selected according to specific needs and cost factors.

[0196] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing an antioxidant and skin-lightening cosmetic composition, characterized in that: The preparation method of the composition comprises the following steps: S100, raw material pretreatment: The following raw materials are selected: water 45%-55%, ascorbic acid 12%-18%, butylene glycol 9%-11%, dipropylene glycol 4.5%-5.5%, 1,3-propylene glycol 4.5%-5.5%, dicaprylyl carbonate 3.2%-3.8%, propylene glycol dicaprylate / dicaprate 2.2%-2.8%, caprylic / capric triglyceride 1.3%-1.7%, polyglycerol-10 laurate 0.9%-1.1%, β-glucan 0.6%-0.9%, 1,2-hexanediol is added several times Take it in moderation, each time, polysorbate 60 0.75% - 0.95%, glycerin 0.65% - 0.85%, dimethicone 0.65% - 0.85%, glyceryl stearate SE 0.45% - 0.55%, glutathione 0.32% - 0.38%, panthenol 0.09% - 0.11%, polyvinyl pyrrolidone 0.09% - 0.11%, xanthan gum 0.09% - 0.11%, sunflower seed oil 0.09% - 0.11%, carrot root extract, Hu Radish seed oil, β-carotene 0.009%-0.011%, tocopherol 0.007%-0.009%, mung bean seed extract, Asian birch bark extract, Rumex crispus root extract, sodium hyaluronate 0.09%-0.11%, ethylhexylglycerin 0.005%-0.007%, grapefruit fruit extract, flavor 0.055%-0.065%, gluconolactone 0.045%-0.055%, sodium gluconate 0.045%-0. 055%, Pullulan polysaccharide 0.045%-0.055%, Guar Hydroxypropyltrimonium Chloride 0.045%-0.055%, Cyclopentasiloxane 0.045%-0.055%, Dimethicone / Vinyl Dimethicone Crosspolymer 0.045%-0.055%, Ethylhexyl Salicylate 0.045%-0.055%, Phytic Acid 0.045%-0.055%, Disodium EDTA 0.018%-0.022%; The solid raw materials are crushed by a crushing device to control the particle size within the range of 50-100 microns, and the liquid raw materials are filtered through a 0.2-0.45 micron filter membrane. For moisture-sensitive raw materials including but not limited to ascorbic acid, glutathione and tocopherol, after crushing and filtering, they are immediately placed in a sealed container filled with dry inert gas for storage until entering the subsequent preparation steps; S200, active ingredient extraction and preliminary mixing: A solvent system composed of ethanol and water in a ratio of 3:2 is prepared, the pretreated raw material is placed in the solvent system, the overall temperature of the solvent system is stirred at 0-5°C, the stirring speed is 200-300 rpm for 30-40 minutes, the mixed solution is centrifuged for 10-15 minutes in a centrifuge with a rotation speed of 3000-3500 rpm to remove undissolved impurities, and a pure active ingredient mixed solution is obtained, wherein the active ingredient contains easily oxidizable ingredients including but not limited to ascorbic acid and tocopherol, in addition to using the ethanol-water solvent system, an appropriate amount of antioxidant including but not limited to sodium vitamin C is added, and the addition amount is 0.1%-0.3% of the total mass of the active ingredient, and the addition is simultaneously added during the stirring process; S300, liposome / microcapsule preparation: Select lipid material in a ratio of phospholipid to cholesterol of 2:1, use a rotary evaporator at a speed of 100-120 rpm and 40-45° C. to rotary evaporate the lipid material for 20-25 minutes to form a uniform film, then add the prepared active ingredient mixture under nitrogen protection, ultrasonically vibrate at a frequency of 30-35 kHz and a pulse interval of 2-3 seconds for 8-12 minutes, and then pass through a polycarbonate membrane with a pore size of 100-150 nanometers and perform high-pressure homogenization at a pressure of 600-700 bar for 3-4 times, and cool to 30-32° C. after each cycle to form liposomes or microcapsules encapsulating the active ingredients; S400, final mixing: The prepared liposomes or microcapsules are mixed with butylene glycol and propylene glycol solvents, emulsifiers such as Tween-80 and Span-60, hydroxyethyl cellulose, carbomer and other thickeners, and a new cross-linking agent, and stirred at a speed of 350-400 rpm for 15-20 minutes, and then the stirring speed is gradually reduced to 100-150 rpm within 15-20 minutes, and the stirring is continued for 30-40 minutes to fully mix the ingredients; S500, Packaging: The packaging containers such as glass bottles or plastic bottles are sterilized with high-temperature steam at a temperature of 121°C for 15-20 minutes. In a clean workshop with a cleanliness level of 10,000, the mixed cosmetic composition is filled into the container at a speed of 5-10 ml per second. After filling, nitrogen is immediately used for sealing.

2. The method for preparing the antioxidant skin-lightening cosmetic composition according to claim 1, characterized in that: The composition preparation method also includes the following steps: S600, Equipment Calibration and Maintenance: The agitator, rotary evaporator and high-pressure homogenizer are calibrated every 1-2 months. The calibration content includes but is not limited to the rotation speed, temperature and pressure. Standard measuring instruments are used for testing to ensure that the parameter error is within ±5%. At the same time, the equipment is cleaned every week and the key components of the equipment are replaced every 3-6 months, including but not limited to the stirring paddle, evaporating flask and homogenizing valve. Before using the equipment each time, run it idle for 5-10 minutes to check whether the equipment is operating normally. Record the use parameters and maintenance status of the equipment each time, including but not limited to calibration time, maintenance items and parts replacement information.

3. The method for preparing the antioxidant skin-lightening cosmetic composition according to claim 1, characterized in that: The composition preparation method also includes the following steps: S700, Environmental Control: During the entire preparation process, the humidity of the preparation environment is controlled at 45%-55%, and the temperature is controlled at 20-23°C. Solid raw materials are stored in a special dry, light-proof warehouse. The warehouse temperature is maintained at 18-20°C and the relative humidity is less than 40%. Liquid raw materials are stored in a cool place with a temperature not exceeding 25°C. S800, Preparation before quality inspection: After the final mixing and preparation is completed, the mixed cosmetic composition is subjected to a preliminary standing treatment for 12-24 hours.

4. The method for preparing the antioxidant skin-lightening cosmetic composition according to claim 1, characterized in that: The composition preparation method also includes the following steps: S900, quality inspection: The content of active ingredients in the cosmetic composition is determined by high performance liquid chromatography according to specific detection conditions including but not limited to mobile phase composition, flow rate and column temperature. The particle size distribution of liposomes or microcapsules is detected by dynamic light scattering, which requires that the particle size is mainly concentrated in the range of 100-200 nanometers and the distribution uniformity meets certain standards. The plate count method is used to detect microbial limits according to national standards. The total number of colonies, molds and yeasts per gram of cosmetics should be lower than the specified limit. The pH value of the cosmetic composition is measured using a pH meter, and the pH value is between 4 and 7; S1000, rework and finished product packaging: Based on the quality test results, batches that do not meet the standards for active ingredient content, particle size distribution, microbial limits or pH value will be reworked. The rework process includes re-extraction of active ingredients, re-preparation of liposomes / microcapsules or mixing and blending until all test indicators are qualified. Only products that pass all test items can be packaged for market sale. The product name, ingredients, shelf life and production batch information should be marked on the packaging.

5. The method for preparing the antioxidant skin-lightening cosmetic composition according to any one of claims 1 to 4, characterized in that: In the S200 active ingredient extraction and preliminary mixing step, the active ingredient contains easily oxidized ingredients including but not limited to ascorbic acid and tocopherol. In addition to adding sodium vitamin C as an antioxidant, inert gas nitrogen is introduced to protect the mixed solution. The introduction rate of the inert gas is controlled at 0.1-0.3 liters / minute, and it is continuously introduced throughout the entire process from the start of stirring to the full dissolution of the active ingredient and the completion of centrifugal treatment.

6. The method for preparing the antioxidant skin-lightening cosmetic composition according to any one of claims 1 to 4, characterized in that: In the S300 liposome / microcapsule preparation step, when the high-pressure homogenization method is used, the homogenization pressure and the number of cycles are adjusted according to the characteristics of the active ingredients and the required liposome or microcapsule performance. Specifically, the homogenization pressure is increased to 700-800 bar and the number of cycles is increased to 4-6 times. During the high-pressure homogenization process, the sealing parts of the homogenization equipment are inspected and maintained every 1-2 cycles.

7. The method for preparing the antioxidant skin-lightening cosmetic composition according to any one of claims 1 to 4, characterized in that: In the final mixing and preparing step S400, the consistency of the cosmetic composition needs to be adjusted, and different types and amounts of thickeners need to be added, including but not limited to adding an appropriate amount of guar gum when a higher consistency is required, and the added amount is 0.5%-1% of the total mixed material. It is slowly added and fully mixed during the stirring process. After adding the thickener, continue stirring for 10-15 minutes.

8. The method for preparing the antioxidant skin-lightening cosmetic composition according to any one of claims 1 to 4, characterized in that: In the S500 packaging step, for the packaging of export products, in addition to the conventional sealing method, an additional layer of moisture-proof and oxygen-proof aluminum foil packaging bag is wrapped outside the packaging container and heat-sealed. At the same time, before packaging, the packaging container and the aluminum foil packaging bag are sterilized with ultraviolet light for 5-10 minutes, and the ultraviolet light intensity is controlled at 1000-1500μW / cm 2 .

9. Use of the antioxidant skin-lightening cosmetic composition prepared by the method for preparing the antioxidant skin-lightening cosmetic composition according to any one of claims 1 to 8.

Images