Anti-hair loss composition and application thereof in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-hair loss composition and application thereof in cosmetics. Specifically, the preparation method comprises the following steps: taking sodium hyaluronate disaccharide amp; the composition is prepared by compounding dimethyl silanol hyaluronate (DSHA for short), lactic acid bacteria / mung bean seed fermentation product filtrate and an acanthopanax senticosus extract. The composition is used as a main effective component to prepare hair care cosmetics. Experiments prove that the traditional Chinese medicine composition has a good effect of improving hair loss. The composition can be used in cosmetics to improve hair loss, so that the composition has a good application prospect in the field of hair care cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an anti-hair loss composition and application thereof in cosmetics. Background Art

[0002] The information disclosed in the background of the invention is only intended to enhance the understanding of the overall background of the invention and should not be necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] According to data published by the American Hair Loss Association, more than 95% of male hair loss is caused by androgenic alopecia. Similarly, data published by the International Society of Hair Restoration Surgery estimates that nearly 40% of men will experience some degree of hair loss at the age of 35, 65% at the age of 60, 70% at the age of 80, and 80% at the age of 85. The increasing prevalence of hair loss is one of the main factors driving the hair loss treatment market. According to data published in the Journal of Clinical and Diagnostic Research (JCDR), approximately 60% of the global population has hair thinning problems, and androgenic alopecia / pattern hair loss is the most common form of hair loss in both men and women.

[0004] The Wnt signaling pathway is the most basic signaling pathway that controls hair development. β-catenin, as the core protein of this pathway, plays a key role in the induction and proliferation of hair follicle papilla cells. It is highly expressed in hair matrix cells, hair papilla cells, and outer root sheath cells. When the Wnt signaling pathway is inhibited, the intracellular β-catenin is degraded, the content of free β-catenin is reduced, and it cannot enter the cell nucleus to perform this function. VEGF is an important mediator of hair follicle growth and angiogenesis, which can improve the formation of blood vessels around hair follicles, thereby increasing hair growth and enlarging hair follicles. Excessive levels of TGF-β2 can significantly impair hair follicle generation and growth. Androgen receptor AR is a nuclear transcription factor. When DHT binds to it, it activates the AR signaling pathway, allowing AR to enter the cell nucleus and bind to the promoter region of the downstream target gene to initiate the transcription of the gene. Among them, DKK1 is a typical downstream target gene. After transcription and synthesis, DKK1 further negatively regulates the Wnt pathway, thereby inhibiting cell proliferation and promoting hair loss.

[0005] Minoxidil and finasteride are drugs approved by FAO for the treatment of hair loss, but the efficacy of these two drugs is limited and short-lived, and is accompanied by unpredictable side effects, such as weight gain, edema, angina pectoris and sexual dysfunction, and these two ingredients are not allowed to be used in cosmetic products. Therefore, it is urgent to find a highly safe and highly effective substitute. However, the inventors found that the cosmetics currently on the market with anti-hair loss efficacy are relatively poor in anti-hair loss effect. Therefore, it is necessary to develop an effective anti-hair loss cosmetic for improving the problem of hair loss. Summary of the invention

[0006] In view of the deficiencies of the prior art, the present invention provides an anti-hair loss composition and its application in cosmetics. Specifically, the present invention prepares a composition by compounding sodium hyaluronate disaccharide & dimethylsilanol hyaluronate (DSHA for short), lactic acid bacteria / mung bean seed fermentation product filtrate, and Acanthopanax senticosus extract; and then uses the composition as the main active ingredient to prepare a hair care cosmetic. It has been verified by experiments that it has a good effect of improving hair loss. Based on the above research results, the present invention is completed.

[0007] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0008] The first aspect of the present invention provides an anti-hair loss composition, which comprises the following components in parts by weight:

[0009] Sodium hyaluronate disaccharide or DSHA 0.01-1.0 parts, lactic acid bacteria / mung bean seed fermentation product filtrate 1.0-15.0 parts, Acanthopanax senticosus extract 1.0-15.0 parts;

[0010] Furthermore, the anti-hair loss composition comprises the following components in parts by weight:

[0011] Sodium hyaluronate disaccharide or DSHA 0.1-0.6 parts, lactic acid bacteria / mung bean seed fermentation product filtrate 5.0-10.0 parts, Acanthopanax senticosus extract 5.0-10.0 parts.

[0012] Wherein, the sodium hyaluronate disaccharide and DSHA can be obtained in a commercial manner.

[0013] The preparation method of the lactic acid bacteria / mung bean seed fermentation product filtrate comprises:

[0014] The lactic acid bacteria are placed in a liquid fermentation medium containing mung bean powder for fermentation, and the product is obtained after filtering, sterilization and impurity removal.

[0015] Wherein, the lactic acid bacteria may be Lactobacillus plantarum;

[0016] The mung bean powder is obtained by drying, crushing and then passing through a 40-60 mesh sieve; the mass volume fraction of the mung bean powder in the liquid fermentation medium is 1-5%, preferably 2% (w / v, g / mL).

[0017] The liquid fermentation medium also contains glucose, which is beneficial to the fermentation culture of lactic acid bacteria. The mass volume fraction of the glucose in the liquid fermentation medium is 0.5-2%, preferably 1% (w / v, g / mL).

[0018] The specific conditions of the fermentation treatment are: fermentation culture at 25-35°C for 12-72 hours, preferably 48 hours.

[0019] The filtration is carried out by using a ceramic membrane, the pore size of which is 50-200 nm, thereby achieving effective sterilization and impurity removal.

[0020] The preparation method of the Acanthopanax senticosus extract comprises: extracting Acanthopanax senticosus root powder by using ultrasound-assisted subcritical extraction technology to obtain an extract, filtering and concentrating the extract, and then adding glycerol to obtain the extract.

[0021] Specifically, the preparation method comprises:

[0022] S1, grinding and sieving Acanthopanax senticosus root to obtain Acanthopanax senticosus root powder, adding the Acanthopanax senticosus root powder into water for ultrasonic treatment, wherein the material-liquid mass ratio is 1:5-1:10, setting the ultrasonic power to 300-500 W, the temperature to 25-40° C., and the time to 25-40 min;

[0023] S2. The feed liquid prepared in S1 is placed in an extractor, and water 0.5-1.0 times the mass of the feed liquid prepared in S1 is added. The extraction conditions are temperature of 120-140°C, pressure of 0.1-0.8MPa, and time of 30-60min. The extract is filtered, and the filtrate is concentrated to 1 / 12-1 / 16 of the original volume under reduced pressure. Glycerin 1-1.5 times the concentrate is added, and the mixture is stirred and mixed evenly. After filtering, the Acanthopanax senticosus extract is obtained, and the concentration of the extract is 0.4-0.8g / mL (calculated as crude drug amount).

[0024] The present invention has been verified through experiments that the above-mentioned composition can improve the problem of hair loss by increasing the content of vascular endothelial growth factor (VEGF) and the expression of β-catenin gene, and down-regulating the expression of TGF-β2, AR, and DKK1 genes.

[0025] Therefore, the second aspect of the present invention provides the use of the above anti-hair loss composition in the preparation of cosmetics. Obviously, the cosmetics have the effects of improving hair loss, preventing hair loss, etc.

[0026] The third aspect of the present invention provides a cosmetic having the efficacy of improving and preventing hair loss, wherein the cosmetic comprises at least the above-mentioned anti-hair loss composition.

[0027] According to the cosmetics of the present invention, the anti-hair loss composition is added in an amount of 1-35% (w / w), such as 1%, 3%, 5%, 7%, 10%, 15%, 17%, 20%, 25%, 30%, 35%, etc., based on the total mass of the cosmetics, and is not specifically limited herein.

[0028] The cosmetics may also contain any other raw material ingredients permitted in the cosmetics field, including but not limited to emulsifiers, emollients, moisturizers, cooling agents, skin conditioners, thickeners, preservatives, and the like.

[0029] At the same time, the present invention can also be used to prepare different cosmetic dosage forms by reasonably adding the above raw materials, such as essence water, essence milk, essence lotion, shampoo, conditioner, hair mask, etc., which are not specifically limited here.

[0030] Beneficial technical effects of one or more of the above technical solutions:

[0031] (1) The anti-hair loss composition obtained by the above technical solution can significantly increase the content of vascular endothelial growth factor (VEGF) and the expression of β-catenin gene, and downregulate the expression of TGF-β2, AR, and DKK1 genes, thereby achieving an effective anti-hair loss effect;

[0032] (2) Sodium hyaluronate disaccharide or DSHA has a high cost. The present invention uses a combination of sodium hyaluronate disaccharide or DSHA, lactic acid bacteria / mung bean seed fermentation product filtrate, and Acanthopanax senticosus extract. When achieving a similar or more significant effect of improving hair loss, the amount of sodium hyaluronate disaccharide or DSHA used is lower, which greatly saves costs.

[0033] (3) The preparation method of the anti-hair loss composition skin care cosmetics obtained by the above technical solution is simple and easy, saves costs, and has good efficacy in improving hair loss through human efficacy experiments, so it has good practical application value. DETAILED DESCRIPTION

[0034] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0035] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0036] The present invention is further described in conjunction with specific examples. The following examples are only for explaining the present invention and are not intended to limit the content thereof. If the specific experimental conditions are not specified in the examples, they are usually carried out according to conventional conditions or the conditions recommended by the reagent company; the reagents and consumables used in the following examples, unless otherwise specified, can all be obtained from commercial sources.

[0037] The present invention is further explained by the following examples, but it does not constitute a limitation of the present invention. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. The test methods for which specific conditions are not specified in the following examples are usually carried out under conventional conditions. It should be noted that the sodium hyaluronate disaccharide, sodium hyaluronate (800,000 Da) and sodium hyaluronate (200,000-400,000 Da) in the examples of the present invention and the comparative examples are purchased from Shandong Focus Freda Biological Co., Ltd., and the trade name of sodium hyaluronate disaccharide is: Huanling 400, INCI name: hydrolyzed sodium hyaluronate. The INCI names of sodium hyaluronate (800,000 Da) and sodium hyaluronate (200,000-400,000 Da) are both sodium hyaluronate. The DSHA in the examples of the present invention and the comparative examples comes from Shandong Freda Biological Co., Ltd., trade name: silanized hyaluronic acid, INCI name: dimethylsilanol hyaluronate. The Lactiplantibacillus plantarum was purchased from the China Industrial Microbiological Culture Collection Center, and its strain collection number is: CICC 20283

[0038] The lactic acid bacteria / mung bean seed fermentation product filtrate in the examples and comparative examples of the present invention is obtained by the following method:

[0039] The suspension of Lactobacillus plantarum CICC 20283 was inoculated into MRS medium (lactic acid bacteria medium, MacLean) and cultured in a 30°C incubator for about 24 h. 600 When the concentration reaches 2.0, the inoculum is inoculated at 2% (V / V) into a sterilized fermentation medium containing water, 2% (w / v) Chongqing mung bean powder (dried, crushed, and passed through a 60-mesh sieve) and 1% (w / v) glucose, and the fermentation is cultured in a 30°C incubator for about 48 hours to obtain a lactic acid bacteria / mung bean seed fermentation product. The fermentation product is then filtered through a ceramic membrane with a pore size of 200 nm, and a lactic acid bacteria / mung bean seed fermentation product filtrate is obtained after sterilization and impurity removal.

[0040] In the examples and comparative examples of the present invention, the Acanthopanax senticosus extract is prepared by the following method:

[0041] The specific steps of the preparation method of Acanthopanax senticosus extract are as follows:

[0042] (1) The dried Acanthopanax senticosus root was crushed and passed through a 40-mesh sieve. An appropriate amount of Acanthopanax senticosus root powder was accurately weighed and added to water with a material-liquid mass ratio of 1:8. The ultrasonic power was set to 400 W, the temperature to 25 °C, and the time to 30 min. After the ultrasonication was completed, it was set aside for use.

[0043] (2) The prepared solution in (1) was placed in an extractor, and water in an amount 0.8 times the mass of the solution prepared in S1 was added. The extraction conditions were as follows: temperature 130° C., pressure 0.5 MPa, and time 40 min. The extract was filtered, and the filtrate was concentrated under reduced pressure to about 1 / 15 of the original volume. Glycerol in an amount 1.5 times the mass of the concentrate was added, and the mixture was stirred and mixed evenly. After filtering, the Acanthopanax senticosus extract was obtained, and the concentration of the extract was 0.5263 g / mL (calculated as crude drug amount).

[0044] Examples 1-6 and Comparative Examples 1-12:

[0045] The specific scheme is shown in Table 1 below, calculated by mass fraction. The preparation method is: sodium hyaluronate disaccharide and DSHA, sodium hyaluronate (800,000 Da), sodium hyaluronate (200,000-400,000 Da), lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract are mixed according to the amounts used in each embodiment and comparative example, and dissolved in purified water.

[0046] Table 1 Specific examples and comparative examples ingredient list

[0047]

[0048]

[0049] Example 7: Anti-hair loss essence

[0050] The above composition is used to prepare an essence product containing the anti-hair loss composition, and the formula is shown in Table 2.

[0051] Table 2 Anti-hair loss essence product formula

[0052]

[0053] Preparation method of anti-hair loss essence:

[0054] S1: Add the raw materials in phase A respectively, heat to 80-85℃, stir and dissolve, and start cooling after all dissolved;

[0055] S2: When the temperature drops to about 50-55°C, add the B phase ingredients, stir and disperse evenly, and continue to cool after dissolution;

[0056] S3: When the temperature drops to 40-45°C, add the C phase ingredients, stir to dissolve and disperse evenly, and the essence milk product is obtained.

[0057] The formula of Comparative Example 13 is the same as that of Example 7. Comparative Example 9 does not contain the B phase component, and the preparation method is the same as that of Example 7.

[0058] Experimental Example 1: VEGF, TGF-β2, β-catenin, AR, DKK1 gene expression experiment

[0059] The experiment used hair papilla cells as a model cell line. According to the test grouping in Table 3, when the cell plating rate in the 6-well plate reached 80-90%, the grouping and drug administration were carried out, and 3 replicate wells were set up in each group. 2 mL of culture medium was added to each well of the blank control (BC) group, 2 mL of culture medium containing 800 nM DHT (dihydrotestosterone, Sigma) was added to each well of the negative control (NC) group, 2 mL of culture medium containing 800 nM DHT and 500 μM minoxidil was added to each well of the positive control (PC) group, and 2 mL of culture medium containing 800 nM DHT and the corresponding concentration of the test substance was added to each well of the sample group. After the drug administration was completed, the 6-well plate was placed in an incubator (37°C, 5% CO2) and cultured for 24 hours.

[0060] ELISA test: After the incubation, the culture medium was collected in a centrifuge tube. After collection, the samples for ELISA detection were placed in a -80°C refrigerator for freezing and stored. The detection and analysis were performed according to the operating instructions of the ELISA kit (Abcam).

[0061] Gene expression detection: After incubation, discard the old solution, wash twice with 1 mL / well PBS (Solabo), add 1 mL AG RNAex Pro Reagent (Acori Biotech) to each well, blow and lyse the cells, and collect the samples. Extract RNA, reverse transcribe to cDNA, and perform fluorescence quantitative PCR detection. -△△CT Method to calculate the results.

[0062]

[0063] GraphPad Prism was used for graphing, and the results were expressed as Mean ± SD. The t-test was used for statistical analysis between the groups. All statistical analyses were two-tailed. 0.01<P<0.05 was considered to be significantly different, and P<0.01 was considered to be extremely significantly different. Compared with the NC group, significance was indicated by *, P-value<0.05 was indicated by *, and P-value<0.01 was indicated by **.

[0064] Table 3 Test plan

[0065]

[0066]

[0067] Note: Sample concentration is 5 mg / mL (diluted with deionized water).

[0068] Table 4 Summary of VEGF content detection results

[0069]

[0070]

[0071] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with blank control; * indicates p<0.01 compared with blank control <p<0.05。)

[0072] As can be seen from Table 4: Compared with the NC group, the VEGF content in the PC group increased significantly, indicating that the positive control test was effective. Single Acanthopanax senticosus extract and sodium hyaluronate (800,000 Da) did not have the function of promoting VEGF expression. Examples 1 to 6 were able to significantly increase the VEGF content, indicating that sodium hyaluronate disaccharide or DSHA can have a significant synergistic effect with lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract, respectively.

[0073] Table 5 Summary of TGF-β2 gene content detection results

[0074]

[0075]

[0076] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with blank control; * indicates p<0.01 compared with blank control <p<0.05。)

[0077] As can be seen from Table 5: Compared with the NC group, the TGF-β2 gene content of the PC group decreased significantly, indicating that the positive control test was effective. Single lactic acid bacteria / mung bean seed fermentation product filtrate and sodium hyaluronate (800,000 Da) do not have the function of reducing TGF-β2 gene expression. Examples 1 to 6 can all significantly reduce the TGF-β2 gene content, indicating that sodium hyaluronate disaccharide or DSHA can have a significant synergistic inhibitory effect with lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract, respectively, and can effectively inhibit TGF-β2 gene expression. Single sodium hyaluronate disaccharide, DSHA, sodium hyaluronate (200,000-400,000 Da) also has the effect of significantly inhibiting TGF-β2 gene expression, but the effect is not as obvious as Examples 1 to 6, and at the same time, sodium hyaluronate (800,000 Da) and sodium hyaluronate (200,000-400,000 Da) have no obvious synergistic effect with Acanthopanax senticosus extract and lactic acid bacteria / mung bean seed fermentation product filtrate.

[0078] Table 6 Summary of β-catenin gene content detection results

[0079]

[0080]

[0081] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with blank control; * indicates p<0.01 compared with blank control <p<0.05。)

[0082] As can be seen from Table 6: compared with the NC group, the β-catenin gene content of the PC group was significantly increased, indicating that the positive control test was effective. Although single sodium hyaluronate (800,000 Da) has a certain effect of promoting β-catenin gene expression, it is not significant, and the synergistic effect between the lactic acid bacteria / mung bean seed fermentation product filtrate and the Acanthopanax senticosus extract is not obvious. Examples 1 to 6 can all significantly increase the β-catenin gene content, indicating that sodium hyaluronate disaccharide and DSHA can respectively have a significant synergistic promotion effect with lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract, and can effectively promote β-catenin gene expression. Single sodium hyaluronate disaccharide, DSHA, sodium hyaluronate (200,000-400,000 Da) also have the effect of increasing the β-catenin gene content, but the effect is not as obvious as Examples 1 to 6, and the synergistic effect of sodium hyaluronate (200,000-400,000 Da) with Acanthopanax senticosus extract and lactic acid bacteria / mung bean seed fermentation product filtrate is not obvious.

[0083] Table 7 Summary of AR gene content detection results

[0084] Sample name Mean SD P-value Downward adjustment rate (%, vs NC) BC 1.00 0.08 / / NC 1.66 0.05 0.000## / PC 1.07 0.08 0.000** 35.54 Example 1 1.24 0.06 0.001** 25.30 Example 2 0.91 0.08 0.000** 45.18 Example 3 1.04 0.06 0.000** 37.35 Example 4 1.29 0.06 0.001** 22.29 Example 5 0.98 0.07 0.000** 40.96 Example 6 1.12 0.05 0.000** 32.53 Comparative Example 1 1.4 0.05 0.003** 15.66 Comparative Example 2 1.43 0.05 0.005** 13.86 Comparative Example 3 1.47 0.06 0.0135* 11.44 Comparative Example 4 1.51 0.05 0.021* 9.04 Comparative Example 5 1.32 0.06 0.001** 20.48 Comparative Example 6 1.31 0.05 0.001** 21.08 Comparative Example 7 1.33 0.05 0.001** 19.88 Comparative Example 8 1.35 0.05 0.002** 18.67 Comparative Example 9 1.53 0.06 0.045* 7.83 Comparative Example 10 1.52 0.05 0.026* 8.43 Comparative Example 11 1.39 0.06 0.004** 16.26 Comparative Example 12 1.35 0.06 0.002** 18.67

[0085] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with blank control; * indicates p<0.01 compared with blank control <p<0.05。)

[0086] As can be seen from Table 7: Compared with the NC group, the AR gene content in the PC group decreased significantly, indicating that the positive control test was effective. Examples 1 to 6 can all significantly downregulate the AR gene content, indicating that sodium hyaluronate disaccharide or DSHA can have a significant synergistic inhibitory effect with the lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract, respectively, and can effectively inhibit AR gene expression. Single sodium hyaluronate disaccharide or DSHA also has a significant inhibitory effect on AR gene expression, but the effect is not as obvious as the synergistic effect of the three in Examples 1 to 6. Although single sodium hyaluronate (200,000-400,000 Da) has a certain inhibitory effect on AR gene expression, the significance is weak, and the synergistic effect with the lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract is not obvious.

[0087] Table 8 Summary of DKK1 gene content detection results

[0088] Sample name Mean SD P-value Downward adjustment rate (%, vs NC) BC 1.00 0.05 / / NC 1.62 0.01 0.000## / PC 1.06 0.05 0.000** 34.57 Example 1 1.049 0.06 0.000** 35.25 Example 2 0.64 0.08 0.000** 60.49 Example 3 0.77 0.08 0.000** 52.47 Example 4 0.97 0.06 0.000** 40.12 Example 5 0.59 0.07 0.000** 63.58 Example 6 0.71 0.08 0.000** 56.17 Comparative Example 1 1.27 0.05 0.000** 21.60 Comparative Example 2 1.20 0.05 0.000** 25.92 Comparative Example 3 1.66 0.08 0.439 -2.47 Comparative Example 4 1.42 0.05 0.002** 12.34 Comparative Example 5 1.25 0.06 0.000** 22.84 Comparative Example 6 1.11 0.06 0.000** 31.48 Comparative Example 7 1.20 0.05 0.000** 25.92 Comparative Example 8 1.06 0.06 0.000** 34.57 Comparative Example 9 1.65 0.06 0.441 -1.85 Comparative Example 10 1.37 0.05 0.001** 15.43 Comparative Example 11 1.42 0.06 0.005** 12.34 Comparative Example 12 1.29 0.06 0.001** 20.37

[0089] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with blank control; * indicates p<0.01 compared with blank control <p<0.05。)

[0090] As can be seen from Table 8: Compared with the NC group, the DKK1 gene content of the PC group decreased significantly, indicating that the positive control test was effective. Examples 1 to 6 can all significantly reduce the DKK1 gene content, indicating that sodium hyaluronate disaccharide or DSHA can have a significant synergistic inhibitory effect with lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract, respectively, and can effectively inhibit DKK1 gene expression. Single sodium hyaluronate (800,000 Da) does not have the effect of inhibiting DKK1 gene expression, and the synergistic effect between single sodium hyaluronate (800,000 Da) or sodium hyaluronate (200,000-400,000 Da) and lactic acid bacteria / mung bean seed fermentation product filtrate and Acanthopanax senticosus extract is not obvious.

[0091] Test Example 2: Evaluation of the efficacy of preventing hair loss in human body

[0092] Testing basis: "Technical Specifications for Safety of Cosmetics" (2015 edition) Testing methods for the anti-hair loss efficacy of cosmetics.

[0093] Qualified subjects were selected according to the inclusion and exclusion criteria. Those who still met the test conditions after a 2-week washout period were randomly divided into a test group and a control group. Finally, 30 subjects were confirmed in the test group and 30 subjects in the control group. The subjects were aged 24 to 59 years old, and the test area was the head. Example 7 is a sample used by the test group, and Comparative Example 9 is a sample used by the control group.

[0094] How to use: Use 3mL per day. Apply the essence evenly on the cleaned and dried scalp. Use your fingertips to gently massage for 5 minutes until it is naturally absorbed. No need to rinse. Wait until it dries naturally and then blow dry.

[0095] Detection time: before using the sample (D0), 14 days after using the sample (D14), and 28 days after using the sample (D28).

[0096] Test equipment:

[0097] 1. Professional digital camera: Canon, model 500D, 15.1 million pixels. The parameters including aperture size, sensitivity (ISO) and focal length remained consistent throughout the test.

[0098] 2. Comb: The tooth density is moderate (tooth spacing 0.9-1.1mm), the tooth length is 2.0-3.0cm, and the comb length is not less than 10cm (excluding the comb handle). The comb of the same specification and material must be used throughout the test. After each use, it must be disinfected in accordance with the relevant requirements of the Technical Specifications for Disinfection of Medical Institutions (WS-T 367-2012).

[0099] Data Analysis:

[0100] The definitions of terms and related calculation formulas involved in the test results are as follows:

[0101] Initial value: refers to the basic value when no sample is used.

[0102] Where x = individual parameter measurement value, n = number of valid data

[0103]

[0104] SPSS Statistics 25 was used for statistical analysis, with two-tailed test and test level α = 0.05.

[0105] The measurement data were analyzed by the normal distribution test results and the difference analysis method was selected: if the measured values ​​were normally distributed, the t-test method was used for statistical analysis; if they were non-normally distributed, the rank sum test method was used for statistical analysis.

[0106] Test conclusion:

[0107] According to the data analysis results, if the hair loss count after using the test product is significantly reduced (P<0.05) compared with before use at any visit time point during the trial, the test product is considered to have anti-hair loss effect. If the overall hair density is significantly increased (P<0.05) compared with before use, the test product is considered to have the effect of increasing hair density.

[0108] At any visit time point during the trial, if the difference in hair loss counts before and after (hair loss counts at a certain visit time point after product use - hair loss counts before product use) is significantly lower than that of the control group (P<0.05), the test sample is considered to have a better anti-hair loss effect than the control product; if the difference in overall hair density before and after (hair density at a certain visit time point after product use - hair density before product use) is significantly higher than that of the control group (P<0.05), the test sample is considered to have a better effect in increasing hair density than the control product.

[0109] The test results are shown in Tables 9 to 12.

[0110] Table 9 Descriptive statistical results of hair loss count (anti-hair loss essence n = 30, anti-hair loss essence blank n = 30)

[0111]

[0112] Table 10 Statistical analysis results of hair loss count (anti-hair loss essence n=30, anti-hair loss essence blank n=30)

[0113]

[0114]

[0115] Statistical analysis results: “*”: the difference is statistically significant (P<0.05); “-”: the difference is not statistically significant (P≥0.05).

[0116] Table 11 Descriptive statistics of overall hair density (anti-hair loss essence n=30, anti-hair loss essence blank n=30)

[0117]

[0118] Table 12 Statistical analysis results of overall hair density (anti-hair loss essence n = 30, anti-hair loss essence blank n = 30)

[0119]

[0120] Statistical analysis results: “*”: the difference is statistically significant (P<0.05); “-”: the difference is not statistically significant (P≥0.05).

[0121] It can be seen from Tables 9 to 12 that in the 4-week test period, the anti-hair loss essence, i.e. Example 7, has significantly improved the amount of hair loss and significantly increased the hair density. Therefore, the essence product containing the anti-hair loss composition has good effects of preventing hair loss and increasing hair density.

[0122] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. An anti-hair loss composition, characterized in that The anti-hair loss composition comprises the following components in parts by weight: Sodium hyaluronate disaccharide or DSHA 0.01-1.0 parts, lactic acid bacteria / mung bean seed fermentation product filtrate 1.0-15.0 parts, Acanthopanax senticosus extract 1.0-15.0 parts.

2. The anti-hair loss composition according to claim 1, characterized in that The anti-hair loss composition comprises the following components in parts by weight: Sodium hyaluronate disaccharide or DSHA 0.1-0.6 parts, lactic acid bacteria / mung bean seed fermentation product filtrate 5.0-10.0 parts, Acanthopanax senticosus extract 5.0-10.0 parts.

3. The anti-hair loss composition according to claim 1, characterized in that The preparation method of the lactic acid bacteria / mung bean seed fermentation product filtrate comprises: The lactic acid bacteria are placed in a liquid fermentation medium containing mung bean powder for fermentation treatment, and then filtered, sterilized and impurity removed to obtain the lactic acid bacteria; wherein the lactic acid bacteria is Lactobacillus plantarum.

4. The anti-hair loss composition according to claim 3, characterized in that The mung bean powder is obtained by drying, crushing and then passing through a 40-60 mesh sieve; the mass volume fraction of the mung bean powder in the liquid fermentation medium is 1-5%, preferably 2% (w / v, g / mL); The liquid fermentation medium also contains glucose, and the mass volume fraction of the glucose in the liquid fermentation medium is 0.5-2%, preferably 1% (w / v, g / mL).

5. The anti-hair loss composition according to claim 3, characterized in that The specific conditions of the fermentation treatment are: fermentation culture at 25-35°C for 12-72h, preferably 48h; The filtration is carried out using a ceramic membrane, and the pore size of the ceramic membrane is 50-200 nm.

6. The anti-hair loss composition according to claim 1, characterized in that The preparation method of the Acanthopanax senticosus extract comprises: extracting Acanthopanax senticosus root powder by using ultrasound-assisted subcritical extraction technology to obtain an extract, filtering and concentrating the extract, and then adding glycerol to obtain the extract.

7. The anti-hair loss composition according to claim 6, characterized in that The preparation method of the Acanthopanax senticosus extract comprises: S1, grinding and sieving Acanthopanax senticosus root to obtain Acanthopanax senticosus root powder, adding the Acanthopanax senticosus root powder into water for ultrasonic treatment, wherein the material-liquid mass ratio is 1:5-1:10, setting the ultrasonic power to 300-500 W, the temperature to 25-40° C., and the time to 25-40 min; S2. The feed liquid prepared in S1 is placed in an extractor, and water 0.5-1.0 times the mass of the feed liquid prepared in S1 is added. The extraction conditions are temperature of 120-140°C, pressure of 0.1-0.8MPa, and time of 30-60min. The extract is filtered, and the filtrate is concentrated to 1 / 12-1 / 16 of the original volume under reduced pressure. Glycerin 1-1.5 times the concentrate is added, and the mixture is stirred and mixed evenly. After filtering, the Acanthopanax senticosus extract is obtained, and the concentration of the extract is 0.4-0.8g / mL (calculated as crude drug amount).

8. Use of the anti-hair loss composition according to any one of claims 1 to 7 in the preparation of cosmetics.

9. A cosmetic having the effect of improving hair loss and preventing hair loss, characterized in that: The cosmetic comprises at least the anti-hair loss composition according to any one of claims 1 to 7.

10. The cosmetic according to claim 9, characterized in that The anti-hair loss composition is added in an amount of 1-35% (w / w) based on the total mass of the cosmetic; The cosmetic dosage forms are essence water, essence milk, essence lotion, shampoo, hair conditioner and hair mask.

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