Plant-source insect-expelling and insect-killing organic fertilizer based on fermented soybeans and preparation method thereof

Abstract

The invention belongs to the field of biological organic fertilizers, and particularly discloses a plant-source insect-expelling and insect-killing organic fertilizer based on fermented soybeans and a preparation method of the plant-source insect-expelling and insect-killing organic fertilizer. Soybean residues are fermented through a compound microbial agent, and natural plant extracts are added to serve as synergistic components. The compound microbial agent comprises strains capable of generating volatile insect repellent compounds and strains capable of promoting soybean nutrition degradation, such as producing bacteria of benzoic acid and isoflavone compounds, photosynthetic bacteria and the like. The natural plant extract is derived from mint, lavender or melia azedarach, so that the parasite expelling effect is enhanced. The preparation method comprises the steps of soybean crushing, inoculant inoculation, illumination fermentation, plant extract addition and the like. The final product is rich in micromolecular nutrient substances and insect-repelling active components, is suitable for planting crops such as tomatoes, and can effectively reduce the use of chemical pesticides and promote the development of green agriculture.

Description

Technical Field

[0001] The invention belongs to the field of biological organic fertilizers, and specifically discloses a plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans and a preparation method thereof. Background Art

[0002] As people's pursuit of green, organic and sustainable agriculture increases, the development of environmentally friendly and efficient plant-based insect repellent and insecticide fertilizers has become a research hotspot in the agricultural field.

[0003] Although traditional organic fertilizers can improve soil structure and soil fertility, they are limited in their effectiveness in repelling and killing insects. They have the following main disadvantages:

[0004] Limited effect on insect repelling and insect killing: Traditional organic fertilizers mainly focus on soil improvement and nutrient supply, but are not very effective in insect repelling and insect killing. This has led to farmers still relying heavily on chemical pesticides during the planting process, which in turn has caused environmental pollution and ecological damage.

[0005] Low nutrient release efficiency: Directly using soybean residue as fertilizer makes it difficult for plants to quickly absorb and utilize nutrients such as protein and polysaccharides. This not only reduces the utilization rate of fertilizers, but may also cause nutrient waste and soil pollution.

[0006] Lack of comprehensive functions: Existing organic fertilizers often only have a single soil improvement or nutrient supply function, and lack comprehensive functions such as insect repellent and insecticide. This limits the widespread application of fertilizers in agricultural production and the improvement of overall benefits.

[0007] Strong dependence on chemical pesticides: Due to the inadequacy of traditional fertilizers in repelling and killing insects, farmers have to frequently use chemical pesticides to control pests. This not only increases agricultural production costs, but also poses a potential threat to the quality of agricultural products and human health.

[0008] Therefore, how to combine microbial fermentation technology and natural plant resources to develop organic fertilizers that can not only provide the nutrients needed by plants but also effectively prevent and control pests has become an important direction of current agricultural scientific research. Summary of the invention

[0009] In view of the above problems, the present invention discloses a plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans and a preparation method thereof. The organic fertilizer of the present invention not only has the functions of soil improvement and nutrient supply, but also has comprehensive functions such as insect repellent and insecticidal. This makes the fertilizer have a wider application prospect and higher overall benefits in agricultural production.

[0010] The purpose of the present invention is achieved through the following technical solutions.

[0011] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0012] The composite microbial agent at least comprises a strain capable of producing volatile compounds that have a repelling or killing effect on a variety of plant pests, and a strain capable of promoting the degradation of soybean protein and polysaccharide substances into small molecule nutrients that are easily absorbed by plants.

[0013] Furthermore, in the above-mentioned plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the volatile compounds include one or more of benzoic acid and isoflavone compounds.

[0014] Furthermore, in the above-mentioned plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the natural plant extract is from one or more of mint, lavender or chinaberry.

[0015] Furthermore, in the above-mentioned plant-based insect repellent and insecticide organic fertilizer based on fermented soybeans, the composite microbial agent contains one or more of photosynthetic bacteria, lactic acid bacteria, yeast, Bacillus, actinomycetes and Penicillium.

[0016] Furthermore, in the above-mentioned plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the composite microbial agent is composed of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson.

[0017] The present invention also discloses a method for preparing the above-mentioned plant-derived insect repellent and insecticidal organic fertilizer based on fermented soybeans, comprising the following steps:

[0018] S1 mixes the washed and crushed soybeans with water in a specific ratio.

[0019] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0020] S3 adds natural plant extracts and mixes them evenly to obtain finished organic fertilizers.

[0021] Furthermore, the soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2-3.

[0022] Furthermore, in step S2, the number of each bacteria in the initial fermentation liquid is not less than 1x10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 30-35°C, the humidity is 80-90%, the stirring speed is 20-30rpm, the fermentation liquid depth is 100-150cm, the light illumination is 2000-6000lux, and the fermentation time is 48-72h.

[0023] Furthermore, the natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.1-0.5wt%.

[0024] The invention also discloses application of the organic fertilizer in planting tomatoes.

[0025] Compared with the existing technology, the present invention has the following advantages and beneficial effects:

[0026] The invention discloses a plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans and a preparation method thereof. The invention adopts a composite microbial agent to ferment soybean residues. The specific strains in the composite microbial agent can promote the degradation of soybean protein and polysaccharide substances, and convert them into small molecule nutrients that are easy for plants to absorb. This not only improves the bioavailability of nutrients, but also accelerates the degradation process of fertilizers, reduces nutrient waste and soil pollution. The organic fertilizer of the invention not only has the functions of soil improvement and nutrient supply, but also has comprehensive functions such as insect repellent and insecticidal. This makes the fertilizer have a wider application prospect and higher overall benefit in agricultural production. Since the organic fertilizer of the invention has a significant insect repellent and insecticidal effect, farmers can reduce or avoid the use of chemical pesticides to control pests. This not only reduces the cost of agricultural production, but also reduces the pollution of chemical pesticides to the environment and agricultural products, and improves the quality and safety of agricultural products. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Comparison of tomato survival rate % of Examples 1-3 and Comparative Examples 1-3 and Control Example;

[0028] Figure 2 Comparison of tomato disease rates % in Examples 1-3, Comparative Examples 1-3 and the control example;

[0029] Figure 3 Comparison of the number of days from planting to maturity of tomatoes in Examples 1-3 and Comparative Examples 1-3 and the control example;

[0030] Figure 4 Comparison of tomato yield per mu (kg) of Examples 1-3, Comparative Examples 1-3 and the control example;

[0031] Figure 5 Comparison of the vitamin C content (mg / kg) of tomatoes in Examples 1-3, Comparative Examples 1-3 and the control example;

[0032] Figure 6 Comparison of lycopene content (ppm) of tomatoes in Examples 1-3, Comparative Examples 1-3 and the control example. DETAILED DESCRIPTION

[0033] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0034] The composite microbial agent consists of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson.

[0035] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0036] S1 mixes the washed and crushed soybeans with a specific proportion of water.

[0037] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0038] S3 adds natural plant extracts and mixes them evenly to produce finished organic fertilizers.

[0039] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2-3.

[0040] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 30-35°C, the humidity is 80-90%, the stirring speed is 20-30rpm, the fermentation liquid depth is 100-150cm, the light illumination is 2000-6000lux, and the fermentation time is 48-72h.

[0041] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.1-0.5wt%.

[0042] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below through the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0043] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0044] Example 1

[0045] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0046] The composite microbial agent is composed of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson;

[0047] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0048] S1 mixes the washed and crushed soybeans with a specific proportion of water.

[0049] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0050] S3 adds natural plant extracts and mixes them evenly to obtain finished organic fertilizers.

[0051] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2.

[0052] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 30°C, the humidity is 80%, the stirring speed is 20rpm, the fermentation liquid depth is 100cm, the light illumination is 2000lux, and the fermentation time is 48h.

[0053] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.1wt%.

[0054] Example 2

[0055] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0056] The composite microbial agent consists of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson.

[0057] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0058] S1 mixes the washed and crushed soybeans with water in a specific ratio.

[0059] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0060] S3 adds natural plant extracts and mixes them evenly to obtain finished organic fertilizers.

[0061] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2.5.

[0062] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 32°C, the humidity is 85%, the stirring speed is 25rpm, the fermentation liquid depth is 125cm, the light illumination is 4000lux, and the fermentation time is 64h.

[0063] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.25wt%.

[0064] Example 3

[0065] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0066] The composite microbial agent consists of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson.

[0067] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0068] S1 mixes the washed and crushed soybeans with a specific proportion of water.

[0069] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0070] S3 adds natural plant extracts and mixes them evenly to produce finished organic fertilizers.

[0071] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:3.

[0072] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 35°C, the humidity is 90%, the stirring speed is 30rpm, the fermentation liquid depth is 150cm, the light illumination is 6000lux, and the fermentation time is 72h.

[0073] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.5wt%.

[0074] Comparative Example 1

[0075] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0076] The composite microbial agent consists of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila and Bacillus licheniformis.

[0077] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0078] S1 mixes the washed and crushed soybeans with water in a specific ratio.

[0079] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0080] S3 adds natural plant extracts and mixes them evenly to obtain finished organic fertilizers.

[0081] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2.5.

[0082] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 32°C, the humidity is 85%, the stirring speed is 25rpm, the fermentation liquid depth is 125cm, the light illumination is 4000lux, and the fermentation time is 64h.

[0083] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.25wt%.

[0084] This comparative example does not contain S. somaliensis and Paecilomyces lilacinus (Thom.) Samson, and the rest is the same as Example 2.

[0085] Comparative Example 2

[0086] A plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans, the organic fertilizer comprising soybean residues fermented with a composite microbial agent and natural plant extracts as synergistic ingredients;

[0087] The composite microbial agent consists of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus and Candida oleophila.

[0088] The method for preparing the above-mentioned plant-derived insecticidal organic fertilizer based on fermented soybeans comprises the following steps:

[0089] S1 mixes the washed and crushed soybeans with water in a specific ratio.

[0090] S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder;

[0091] S3 adds natural plant extracts and mixes them evenly to obtain finished organic fertilizers.

[0092] The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2.5.

[0093] In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 32°C, the humidity is 85%, the stirring speed is 25rpm, the fermentation liquid depth is 125cm, the light illumination is 4000lux, and the fermentation time is 64h.

[0094] The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.25wt%.

[0095] This comparative example does not contain Bacillus licheniformis, S. somaliensis and Paecilomyces lilacinus (Thom.) Samson, and the rest is the same as Example 2.

[0096] Comparative Example 3

[0097] This comparative example does not contain natural plant extracts, and the rest is the same as Example 2.

[0098] Test Case

[0099] The organic fertilizers prepared in Examples 1-3 and Comparative Examples 1-3 were used for the cultivation of cherry tomatoes. The greenhouse was evenly divided into 7 small plots (including a comparative example, using ordinary farmyard manure), each with an area of ​​9 square meters. The organic fertilizers of Examples 1-3 and Comparative Examples 1-3 were used in each small plot of planting test field to plant cherry tomatoes. The cherry tomato varieties used in the planting test were all Busan 88. The planting method includes the following methods:

[0100] 1. Seed treatment

[0101] Drying and soaking: Dry the cherry tomato seeds for 2 to 4 hours (avoid exposure to the sun) to increase the germination rate. Afterwards, you can choose to soak the seeds in 50-55°C hot water for 20-30 minutes, or soak them in clean water for 4-6 hours, and then treat them with 800 times chlorothalonil or 1200 times thiophanate-methyl solution for 15-20 minutes to disinfect and sterilize.

[0102] 2. Seedling cultivation

[0103] Seedbed preparation: The seedbed field needs to be turned and dried at least one week in advance, raked and leveled before sowing, and sufficient base fertilizer should be applied.

[0104] Use well-rotted farmyard manure as base fertilizer;

[0105] Sowing: Sow the treated seeds evenly on the seedbed, cover with about 0.5 cm thick fine soil, and spray water to keep moisture. Depending on the season, you can take measures such as sunshade nets to prevent sunburn and moisturize or cover the small arch shed with plastic film to keep warm and moisturize.

[0106] Seedling management: When the seedlings grow to 5 to 6 leaves, they can be transplanted to the field.

[0107] 3. Planting

[0108] Site selection: Choose deep, fertile, well-drained sandy loam as the planting site. The pH value of the soil should be between 6.8 and 7.5.

[0109] Application of basal fertilizer: Before planting, sufficient basal fertilizer should be applied, and decomposed farmyard manure (comparative example) or organic fertilizers of Examples 1-3 of the present invention and Comparative Examples 1-3 should be selected; the basal fertilizer should be evenly spread on the plot, and deep plowing, harrowing and ridge building should be carried out; the fertilizer application rate is 2 kg per square meter.

[0110] Planting method: Plant in windless and sunny weather, and try to transplant with soil to reduce root damage. After planting, water the roots sufficiently and keep the soil moist to promote seedling growth.

[0111] 4. Field Management

[0112] Water management: Before fruiting, control the water content appropriately to prevent the plant from growing too tall. The water requirement is high during the fruiting period, so the soil should be kept moist as much as possible. Be careful to avoid the soil being too dry or too wet, which may cause diseases.

[0113] Fertilization management: Apply topdressing in time according to the growth of cherry tomatoes. Use urea and other nitrogen fertilizers as seedling fertilizers; use compound fertilizers and foliar fertilizers as fruit-inducing fertilizers.

[0114] The planting results are shown in Table 1 and Figure 1-3 As shown, the survival rate (fixed survival rate), disease rate (fixed disease rate), and days from planting to maturity were calculated.

[0115] Table 1 Tomato planting test

[0116] Survival rate% Disease rate% Days from planting to maturity Example 1 98.2 7.2 69 Example 2 98.7 5.6 67 Example 3 99.2 5.4 67 Comparative Example 1 95.2 9.7 70 Comparative Example 2 93.1 10.3 71 Comparative Example 3 92.8 16.4 74 Comparison Example 87.2 27.1 78

[0117] It can be seen from the data in Table 1 that the use of the plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans as the base fertilizer for cherry tomatoes after valorization can significantly improve their survival rate, significantly reduce the disease rate and shorten the number of days to maturity after planting, thereby reducing the planting cost, compared with ordinary farmyard manure.

[0118] From the comparison between Example 2 and Comparative Examples 1-3, it can be seen that the composite microbial agent used in the fermentation of the present invention in combination with azadirachtin can improve the survival rate and disease rate of cherry tomatoes.

[0119] Furthermore, after the cherry tomatoes are harvested, the per-acre yield (converted), vitamin C content and lycopene content of cherry tomatoes are calculated to reflect the overall quality of the fruit.

[0120] The results are shown in Table 2 and Figure 4-6 shown.

[0121] Table 2 Tomato fruit quality test

[0122]

[0123] From the results in Table 2, it can be seen that the use of the plant-derived insecticidal organic fertilizer based on fermented soybeans as the base fertilizer for cherry tomatoes after valorization can significantly increase the yield per mu, vitamin C content and lycopene content compared to ordinary farmyard manure. From the comparison between Example 2 and Comparative Examples 1-3, the composite microbial agent used in the fermentation of the present invention and azadirachtin can improve the fruit quality of cherry tomatoes.

[0124] From the above embodiments, it can be seen that the present invention discloses a plant-based insect repellent and insecticidal organic fertilizer based on fermented soybeans and a preparation method thereof. The present invention uses a composite microbial agent to ferment soybean residues. The specific strains in the composite microbial agent can promote the degradation of soybean protein and polysaccharide substances, and convert them into small molecule nutrients that are easy for plants to absorb. This not only improves the bioavailability of nutrients, but also accelerates the degradation process of fertilizers, reduces nutrient waste and soil pollution. The organic fertilizer of the present invention not only has the functions of soil improvement and nutrient supply, but also has comprehensive functions such as insect repellent and insecticidal. This makes the fertilizer have a wider application prospect and higher overall benefits in agricultural production. Since the organic fertilizer of the present invention has a significant insect repellent and insecticidal effect, farmers can reduce or avoid the use of chemical pesticides to control pests. This not only reduces the cost of agricultural production, but also reduces the pollution of chemical pesticides to the environment and agricultural products, and improves the quality and safety of agricultural products.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Therefore, based on the innovative concept of the present invention, changes and modifications to the embodiments described herein, or equivalent structural or equivalent process transformations made using the contents of the specification and drawings of the present invention, directly or indirectly applying the above technical solutions to other related technical fields are all included in the protection scope of the patent of the present invention.

Claims

1. A plant-derived insecticidal organic fertilizer based on fermented soybeans, characterized in that: The organic fertilizer contains soybean residues fermented by composite microbial agents and natural plant extracts as synergistic ingredients; The composite microbial agent at least comprises a strain capable of producing volatile compounds that have a repelling or killing effect on a variety of plant pests, and a strain capable of promoting the degradation of soybean protein and polysaccharide substances into small molecule nutrients that are easily absorbed by plants.

2. A plant-derived insecticidal organic fertilizer based on fermented soybeans according to claim 1, characterized in that: The volatile compounds include one or more of benzoic acid and isoflavone compounds.

3. A plant-derived insecticidal organic fertilizer based on fermented soybeans according to claim 1, characterized in that: The natural plant extract is from one or more of mint, lavender or chinaberry.

4. The plant-derived insecticidal organic fertilizer based on fermented soybean according to claim 1, characterized in that: The composite microbial agent contains one or more of photosynthetic bacteria, lactic acid bacteria, yeast, bacillus, actinomycetes and penicillium.

5. The plant-derived insecticidal organic fertilizer based on fermented soybean according to claim 1, characterized in that: The composite microbial agent is composed of Rhodopseudomonas sphaeroides, Lactobacillus acidophilus, Candida oleophila, Bacillus licheniformis, S. somaliensis) and Paecilomyces lilacinus (Thom.) Samson.

6. The method for preparing the plant-derived insecticidal organic fertilizer based on fermented soybeans according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1 mixes the washed and crushed soybeans with a specific proportion of water. S2 inoculates the composite microbial agent, and ferments the soybeans in a fermentation device under light conditions until the soybeans are fully degraded and produce the desired bioactive substances, filters to remove most of the water, and freeze-dries to obtain freeze-dried powder; S3 adds natural plant extracts and mixes them evenly to produce finished organic fertilizers.

7. The preparation method according to claim 6, characterized in that: The soybeans in step S1 are dried to have a moisture content of less than 12%, the crushed soybeans are sieved through a 50-mesh sieve, and the mass ratio of the soybeans to water is 1:2-3.

8. The preparation method according to claim 6, characterized in that: In step S2, the number of each bacteria in the initial fermentation liquid is not less than 1×10 5 / mL; during the fermentation process, the fermentation device is a circular open fermentation tank, the ambient temperature is 30-35°C, the humidity is 80-90%, the stirring speed is 20-30rpm, the fermentation liquid depth is 100-150cm, the light illumination is 2000-6000lux, and the fermentation time is 48-72h.

9. The preparation method according to claim 6, characterized in that: The natural plant extract is azadirachtin, and the content of the azadirachtin in the final organic fertilizer is 0.1-0.5wt%.

10. Use of the organic fertilizer according to claims 1-5 in growing tomatoes.

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