A method for producing seed of a matching line of egg-laying green-shell Pu'an Panjiang black-bone chicken

Abstract

The present invention discloses a method for producing a pair of egg-laying green-shell Pu'an Panjiang black-bone chicken seed lines. The method introduces the bloodlines of red-feathered black-tailed chickens and Zhuxiang chickens on the basis of the bloodline of Pu'an Panjiang black-bone chickens, and adopts a three-line matching method to form a ZX×(PA×HH) combination, so that the commercial egg-laying green-shell Pu'an Panjiang black-bone chicken retains the "five blacks" Pu'an Panjiang black-bone chicken breed characteristics of "black feathers, black skin, black shanks, black crowns, and black meat" and the market weight. The production economic benefits are higher than those of green-shell eggs, and the egg production and egg weight are significantly improved, and the production performance is further improved. At the same time, the egg-laying green-shell Pu'an Panjiang black-bone chicken pairing obtained by the present invention has an ideal appearance, a medium body shape, a high egg production, a large egg weight, and produces green-shell eggs. The market age and market weight are similar to those of purebred Pu'an Panjiang black-bone chickens, and the egg production performance, egg weight, and eggshell color are all better than those of purebred Pu'an Panjiang black-bone chickens. It is suitable for promotion and application in the field of poultry breeding technology, especially the field of Pu'an Panjiang black-bone chicken breeding technology.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a seed production method for a matching line of egg-laying green-shell Pu'an Panjiang black-bone chickens. Background Art

[0002] Innovation in livestock and poultry germplasm resources has become the core driving force for ensuring national food security and driving agricultural transformation and upgrading.

[0003] Local chickens boast excellent meat and egg quality, but their production performance is relatively low. Therefore, while maintaining the appearance and market weight of local chicken breeds, increasing egg production can effectively improve economic benefits throughout the breeding cycle and is one of the cost-reduction and efficiency-enhancing measures to address the persistently high costs of feed ingredients. In the context of consumption upgrades, eggshell color and egg weight, two key indicators influencing consumer choices, are becoming crucial factors in reshaping the market value of eggs. Research has shown that unique eggshell colors (such as the natural turquoise color of green-shelled eggs) not only satisfy consumers' aesthetic needs for product differentiation but also visually convey the quality associations of "high nutrition" and "ecological farming," leading to green-shelled eggs fetching prices 2-4 times higher than regular eggs. Egg weight, on the other hand, directly influences household purchasing decisions. Large-sized eggs over 50g command a 30% premium in niche markets such as baking and maternity. The synergistic improvement of these two traits can not only enhance product value through brand recognition through eggshell color, but also cater to diverse consumer scenarios through egg weight grading. This creates a dual competitive advantage of "sensory value + practical value," creating differentiated value growth for the laying hen industry.

[0004] The Pu'an Panjiang black-bone chicken, also known as the Pu'an Niujiaoshan black-bone chicken and the Pu'an black-gold chicken, is named after its primary production area in the Beipanjiang River basin of Pu'an County, Qianxinan Prefecture, Guizhou Province. Through long-term natural and artificial selection, this unique black-bone chicken has evolved into a versatile, medicinal, and roughage-tolerant black-bone chicken. Its distinctive black feathers, black shanks, black skin, black meat, and black bones, combined with its strong resistance to adversity and its delicious, flavorful meat and eggs, have made it a staple of the local ecological poultry industry. However, the current level of development and utilization of the Pu'an Panjiang black-bone chicken remains low, with issues such as slow growth, low egg production, and small egg weight severely hindering the high-quality development of the industry. Therefore, it is crucial to implement genetic innovation for this local resource, the Pu'an Panjiang black-bone chicken.

[0005] The Bamboo Chicken, named after its origins in Chishui City, Zunyi City, Guizhou Province, known as the "Hometown of Nanzhu," is a local breed used for both meat and eggs. Due to the local preference for black-bone individuals, they were intentionally selected, resulting in a distinctive appearance characterized by black feathers, skin, meat, bones, beaks, combs, and toes. They boast high egg production, with some flocks capable of producing green-shelled eggs. They are a valuable and highly versatile breeding material, though their current development and utilization remains relatively low. The Red-Feathered Black-Tailed Chicken, derived from the commercial Babcock B380 laying hen, is medium-sized with red feathers and a black tail. They produce high-yield, brown eggs of large weight. Crossbreeding with colored-feathered roosters often retains the paternal characteristics, making them a promising breeding material for improving low egg production and egg weight in local breeds.

[0006] Therefore, by utilizing the advantages of the above materials, further hybridization and improvement of the Pu'an Panjiang black chicken can be carried out, while retaining its appearance characteristics, its egg-laying performance can be improved, and the egg-laying green-shell Pu'an Panjiang black chicken that meets the needs of the southwest and south China regions can be cultivated. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to provide a breeding method for a matching system of green-shell Pu'an Panjiang black-bone chickens with ideal appearance, medium body shape, high egg production, large egg weight, green-shell eggs and stable performance.

[0008] The technical solution adopted by the present invention to solve the technical problem is: the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system seed production method comprises the following steps:

[0009] 1) Paternal breeding:

[0010] 101) Select the existing Zhuxiang Chicken ZX line, and breed males that meet the weight and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight and egg production standards at 16 weeks and 43 weeks of age, and the appearance standards. Then, select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight and appearance standards at 16 and 43 weeks of age for males and the weight and egg production standards at 16 weeks and 43 weeks of age for females;

[0011] 102) The ZX paternal line father and the ZX paternal line mother of the great-grandfather generation are mated and selected according to the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, and the weight and egg production standards and appearance standards of hens at 16 weeks and 43 weeks of age, to obtain the ZX paternal line father and the ZX paternal line mother of the grandparent generation;

[0012] 103) The grandparent ZX paternal line father and grandparent ZX paternal line mother were mated and selected based on the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, the semen quality standards of roosters at 30 weeks of age, and the green-shell egg positive homozygous genotype to obtain the parent ZX paternal line father;

[0013] 2) Maternal breeding:

[0014] 201) Breeding roosters of the existing purebred Pu'an Panjiang black-bone chicken PA line that meet the weight and appearance standards at 16 weeks and 43 weeks of age with hens that meet the weight standards at 16 weeks and 43 weeks of age and the egg production and appearance standards at 43 weeks of age, and selecting and breeding according to the egg production and appearance standards of the hens at 43 weeks of age to obtain the great-grandfather generation PA maternal sire and the great-grandfather generation PA maternal dam;

[0015] 202) The great-grandfather PA maternal sire is mated with the great-grandfather PA maternal sire and selected according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire;

[0016] 203) Use the existing red-feathered black-tailed hen HH line that meets the appearance characteristics, egg production at 43 weeks of age, and weight at 16 and 43 weeks of age as the grandparent HH maternal line mother;

[0017] 204) The grandparent generation PA maternal sire and the grandparent generation HH maternal dam are mated and the parent generation PH maternal sire is obtained by selecting the hen based on the egg production at 43 weeks of age and the weight and appearance standards at 16 and 43 weeks of age;

[0018] 3) The ZX paternal parent obtained by paternal selection is crossed with the PH maternal parent obtained by maternal selection to obtain the egg-laying green-shell Pu'an Panjiang black-bone chicken.

[0019] Furthermore, in steps 101) to 103), the selection and breeding of the great-grandfather ZX paternal father, the great-grandfather ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage breeding and artificial insemination.

[0020] Furthermore, in steps 201) to 204), the breeding of the great-grandfather PA maternal father, the great-grandfather PA maternal mother, the grandparent PA maternal father, the grandparent HH maternal mother, and the parent PH maternal mother are all carried out by single cage breeding and artificial insemination.

[0021] Furthermore, in steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 16 weeks of age is 1.75 kg, the weight standard at 43 weeks of age is 2.1 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and solid, feathers with a dark green metallic luster, well-developed sickle feathers, black feathers, black skin, black legs, black crown, and black meat, and the semen quality requirements of the Zhuxiang chicken rooster are a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, and fertilization rate of more than 90%; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs with green eggshell color and an average egg weight of 50 g, and the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3 kg, and the weight at 43 weeks of age is 1.8 kg, the appearance characteristics of the Bamboo Chicken are its sleek body, well-proportioned structure, black feathers, black skin, black legs, black crown and black meat.

[0022] Furthermore, in step 201) and step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is that the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, and fertilization rate more than 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.9 kg, the egg production standard at 43 weeks of age is more than 65 eggs, the egg shell color is pink, and the average egg weight is 48. g. The appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown and black meat.

[0023] Furthermore, in step 203), the appearance characteristics of the red-feathered black-tailed hen HH are a sleek body, red feathers, black tail, yellow shanks, a red single comb and white skin, an average body weight of 1.3 kg at 16 weeks of age, an average body weight of 1.8 kg at 43 weeks of age, an egg production of more than 150 eggs at 43 weeks of age, an average egg weight of 62.5 g, and a brown eggshell color.

[0024] Furthermore, in step 204), the PH hen has black feathers and black skin, a standard weight of 1.2 kg at 16 weeks of age, a standard weight of 1.8 kg at 43 weeks of age, an egg production of 110 eggs at 43 weeks of age, an average egg weight of 55 g, and a light brown eggshell color.

[0025] Furthermore, in step 3), the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "black feathers, black skin, black legs, black crown, and black meat". The egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained have reduced egg production after 43 weeks of age and can be marketed as high-quality old hens; the egg-laying green-shell Pu'an Panjiang black-bone chicken roosters obtained can be marketed as high-quality broilers at 20-22 weeks of age.

[0026] Furthermore, before mating, the breeder chickens in the matching line are purified from salmonella and chicken leukemia.

[0027] The beneficial effects of the present invention are as follows: the egg-laying green-shell Pu'an Panjiang black-bone chicken complementary breeding method introduces the bloodlines of red-feathered black-tailed chickens and Zhuxiang chickens on the basis of the bloodline of Pu'an Panjiang black-bone chickens, and adopts the matching combination ZX×(PA×HH), that is, hybridizing Pu'an Panjiang black-bone chicken roosters with red-feathered black-tailed chicken hens, thereby improving the egg production and egg weight of the offspring under good hybrid advantages; further adopting Zhuxiang chicken roosters as the terminal father, the obtained commercial generation inherits the green-shell egg gene of the terminal father, the hens have excellent egg-laying performance and produce green-shell eggs with higher economic benefits, and can be sold as local specialty high-quality old hens after the egg-laying is completed. The egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention contains 25% of Pu'an Panjiang black-bone chicken bloodline, 50% of Zhuxiang chicken bloodline, and 25% of red-feathered black-tailed chicken bloodline. The commercial generation retains the "five black" appearance characteristics of the purebred Pu'an Panjiang black-bone chicken, namely, black feathers, black skin, black shanks, black crown, and black meat. The adult chickens have ideal body shape and appearance, are completely free of diseases, and have excellent production performance. The present invention gives full play to the high-yield performance of the grandparent maternal red-feathered black-tailed chicken HH line with high egg production and large egg weight, which significantly improves the egg production and egg weight of the egg-laying green-shell Pu'an Panjiang black-bone chicken at 43 weeks of age. It inherits the green-shelled egg gene of the terminal paternal Zhuxiang chicken ZX line, as well as the production performance with higher egg-laying performance, so that the commercial generation hens have excellent egg-laying performance and produce green-shelled eggs with higher economic value. In addition, the egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention retains the "five black" appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat" and the market weight of the purebred Pu'an Panjiang black-bone chicken, which can meet consumers' preference for "five black" chickens. Roosters can be marketed as high-quality broilers at the age of 20-22 weeks, with an average weight of 2 kg, which is similar to the market age and market weight of the purebred Pu'an Panjiang black-bone chicken, and has good meat performance. Furthermore, the egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained by the present invention produce green-shell eggs before 43 weeks of age, and their egg production and average egg weight are significantly greater than those of purebred Pu'an Panjiang black-bone chickens. The average egg production at 43 weeks of age is 102 eggs, 37 more than that of purebred Pu'an Panjiang black-bone chickens, significantly improving egg production. The average egg weight is 52g, 4g heavier than that of purebred Pu'an Panjiang black-bone chickens. The eggshell color is green, which has a higher economic value than white-shell eggs, significantly improving egg quality such as egg weight and eggshell color. Furthermore, hens with the "five black" appearance characteristics, whose egg production decreases after 43 weeks of age, can be marketed as high-quality local specialty hens, further increasing the residual value of culled laying hens. Therefore, the egg-laying green-shell Pu'an Panjiang black-bone chicken obtained by the present invention has an ideal body shape and appearance, is completely free of diseases, and has excellent production performance; the commercial generation of the egg-laying green-shell Pu'an Panjiang black-bone chicken matching series retains the "five black" appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat" and market weight of the purebred Pu'an Panjiang black-bone chicken, has an ideal appearance, a medium body shape, a high egg production rate, a large egg weight, and produces green-shelled eggs. The market age and market weight are similar to those of the purebred Pu'an Panjiang black-bone chicken, and the egg-laying performance, egg weight, and eggshell color are all better than those of the purebred Pu'an Panjiang black-bone chicken.The present invention adopts individual selection and family selection methods, and can accurately select the Pu'an Panjiang black-bone chicken matching line with uniform appearance, high egg production, large egg weight, green-shell eggs and stable performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the egg-laying green-shell Pu'an Panjiang black-bone chicken breeding system seed production method of the present invention;

[0029] Figure 2 This is a diagram showing the appearance characteristics of the egg-laying green-shell Pu'an Panjiang black-bone chicken seed production according to the present invention. DETAILED DESCRIPTION

[0030] like Figure 1-2 As shown, the egg-laying green-shell Pu'an Panjiang black-bone chicken matching seed production method comprises the following steps:

[0031] 1) Paternal breeding:

[0032] 101) Select the existing Zhuxiang Chicken ZX line, and breed males that meet the weight and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight and egg production standards at 16 weeks and 43 weeks of age, and the appearance standards. Then, select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight and appearance standards at 16 and 43 weeks of age for males and the weight and egg production standards at 16 weeks and 43 weeks of age for females;

[0033] 102) The ZX paternal line father and the ZX paternal line mother of the great-grandfather generation are mated and selected according to the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, and the weight and egg production standards and appearance standards of hens at 16 weeks and 43 weeks of age, to obtain the ZX paternal line father and the ZX paternal line mother of the grandparent generation;

[0034] 103) The grandparent ZX paternal line father and grandparent ZX paternal line mother were mated and selected based on the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, the semen quality standards of roosters at 30 weeks of age, and the green-shell egg positive homozygous genotype to obtain the parent ZX paternal line father;

[0035] In steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 16 weeks of age is 1.75 kg, the weight standard at 43 weeks of age is 2.1 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and sturdy, the feathers have a dark green metallic luster, the sickle feathers are well developed, the feathers, black skin, black legs, black crown, and black meat are black, and the semen quality requirements of the Zhuxiang chicken rooster are a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, and fertilization rate 90% or more; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs with green eggshell color and an average egg weight of 50 g, the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3 kg, and the weight standard at 43 weeks of age is 1.8 kg, the appearance characteristics of the Zhuxiang chicken are a sleek body, a well-proportioned structure, black feathers, black skin, black legs, black crown, and black meat. The green-shell egg-positive homozygous rooster is obtained by the following method: according to the characteristic that the green-shell egg trait is autosomal dominant, a group detection of the Zhuxiang chicken is carried out using molecular marker technology, and the green-shell egg-positive homozygous genotype is selected to obtain a ZX line with a green-shell egg rate of 100%; wherein, the molecular marker technology is realized by using the patented technology with application number 201510194180.6, application date 2015-04-22, and patent name "Primer composition for identifying the genotype of green-shell laying hens and its application";

[0036] 2) Maternal breeding:

[0037] 201) The existing purebred Puan Panjiang black-bred chicken PA line that meets the weight and appearance standards of 16 weeks and 43 weeks of age is bred with the hens that meet the weight standards of 16 weeks and 43 weeks of age and the egg production standards and appearance standards of 43 weeks of age, and the great-grandfather generation PA maternal father and great-grandfather generation PA maternal mother are selected according to the egg production standards and appearance standards of the hens at 43 weeks of age; the weight standard of the Puan Panjiang black-bred chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is that the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs, the appearance standard of the Puan Panjiang black-bred chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, and black meat, and the semen quality requirement of the Puan Panjiang black-bred chicken rooster is a single semen collection volume of 0.5 mL or more, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, fertilization rate 90% or more; the Pu'an Panjiang black-bone chicken hen's weight standard at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.9 kg, the egg production standard at 43 weeks of age is 65 or more with pink eggshell color, and the average egg weight is 48 g; the Pu'an Panjiang black-bone chicken hen's appearance standard is medium size, black feathers, black skin, black shanks, black crown, and black meat;

[0038] 202), the great-grandfather PA maternal sire is bred with the great-grandfather PA maternal dam and the grandparent PA maternal sire is selected according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire; the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, and the weight standard at 43 weeks of age is 2.5 kg. The standard for egg production at 43 weeks of age is that the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs. The appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black legs, black crown, and black meat. The semen quality requirements of the Pu'an Panjiang black-bone chicken rooster are a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality above 0.7, and fertilization rate above 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2 kg, and the weight standard at 43 weeks of age is 1.9 kg, the egg production standard at 43 weeks of age is more than 65 eggs with pink eggshell color, and the average egg weight is 48 g. The appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black shanks, black crown, and black meat;

[0039] 203) Use existing red-feathered, black-tailed hens (HH) that meet the appearance characteristics, egg production at 43 weeks of age, and weight at 16 and 43 weeks as the grandparent HH maternal parent; the appearance characteristics of the red-feathered, black-tailed hens (HH) are a sleek body, red feathers, black tail, yellow shanks, a single red comb, and white skin; the average weight at 16 weeks of age is 1.3 kg, the average weight at 43 weeks of age is 1.8 kg, the egg production at 43 weeks of age is more than 150 eggs, the average egg weight is 62.5 g, and the eggshell color is brown;

[0040] 204) Breeding the grandparent PA maternal sire and the grandparent HH maternal dam and selecting the parent PH maternal sire based on the egg production at 43 weeks of age, the weight standards at 16 weeks and 43 weeks of age, and the appearance standards; the PH hens have black feathers and black skin, the weight standard at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.8 kg, the egg production at 43 weeks of age is 110, the average egg weight is 55 g, and the eggshell color is light brown;

[0041] 3) Crossbreeding the ZX sire parent, derived from paternal selection, with the PH dam parent, derived from maternal selection, yields the green-shelled Pu'an Panjiang black-bone chicken. The resulting egg-laying green-shelled Pu'an Panjiang black-bone chicken exhibits the typical Pu'an Panjiang black-bone chicken appearance and market weight, with black feathers, skin, shanks, crown, and flesh. Hens produce green-shelled eggs before 43 weeks of age, averaging 102 eggs and 52g egg weight at 43 weeks. After 43 weeks of age, egg production declines and the chickens are marketed as high-quality older hens, averaging 1.8kg weight. Roosters are marketed as high-quality broilers at 20-22 weeks of age, averaging 2kg weight.

[0042] In the above embodiment, to ensure the systematic breeding of the egg-laying green-shell Pu'an Panjiang black-bone chicken, in steps 101) to 103), the great-grandparent ZX paternal line father, the great-grandparent ZX paternal line mother, the grandparent ZX paternal line father, the grandparent ZX paternal line mother, and the parent ZX paternal line father were all reared in single cages and purebred through artificial insemination. Furthermore, in steps 201) to 204), the great-grandparent PA maternal line father, the great-grandparent PA maternal line mother, the grandparent PA maternal line father, the grandparent HH maternal line mother, and the parent PH maternal line mother were all reared in single cages and hybridized through artificial insemination.

[0043] Furthermore, in order to ensure the healthy production of egg-laying green-shell Pu'an Panjiang black-bone chickens, the breeding chickens in the matching system are purified from Salmonella and chicken leukemia before breeding.

[0044] The present invention has statistically analyzed the overall appearance characteristics, growth performance, egg-laying performance and slaughter performance of the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken. Its appearance characteristics are shown in Table 1, its growth performance is shown in Table 2, its egg-laying performance is shown in Table 3, and its slaughter performance is shown in Table 4:

[0045] Table 1 Appearance characteristics

[0046]

[0047] Table 2 Growth performance

[0048]

[0049] Table 3 Egg production performance

[0050]

[0051] Table 4 Slaughter performance

[0052]

[0053] As shown in Tables 1-4, the commercial flock of the egg-laying green-shell Pu'an Panjiang black-bone chickens features a single purple-black comb, black feathers with a metallic sheen, black shanks, skin, and meat. These flocks retain the "five black" appearance characteristics of the purebred Pu'an Panjiang black-bone chicken—black feathers, skin, shanks, comb, and meat—and their market weight, meeting consumer preferences for "five black" chickens. Hens produce green-shelled eggs before 43 weeks of age. At 43 weeks, they lay an average of 102 eggs, 37 more than purebred Pu'an Panjiang black-bone chickens, significantly improving their egg production. Their average egg weight is 52 g, 4 g heavier than purebred Pu'an Panjiang black-bone chickens. Their eggshell color is green, which is more economically valuable than white-shelled eggs. This significantly improves egg quality, including egg weight and shell color. Furthermore, hens with the "five black" appearance, whose egg production declines after 43 weeks of age, can be marketed as high-quality, local specialty hens, further increasing the residual value of culled laying hens. Roosters at 20-22 weeks of age can be marketed as high-quality broilers with an average weight of 2 kg, which is similar to the market weight and market age of purebred Pu'an Panjiang black-bone chickens, and have good meat performance, meeting the expected results. Therefore, the egg-laying green-shell Pu'an Panjiang black-bone chickens obtained by the present invention have ideal body shape and appearance, complete disease elimination, and excellent production performance; the commercial generation of the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system retains the "five black" appearance characteristics of the purebred Pu'an Panjiang black-bone chicken, namely "black feathers, black skin, black shanks, black crown, and black meat", as well as the market weight. They have ideal appearance, medium body shape, high egg production, large egg weight, and produce green-shell eggs. The market age and market weight are similar to those of purebred Pu'an Panjiang black-bone chickens, and the egg production performance, egg weight, and eggshell color are all better than those of the purebred Pu'an Panjiang black-bone chicken line. Therefore, the present invention can provide better reference information for the breeding of the egg-laying green-shell Pu'an Panjiang black-bone chicken matching system.

[0054] The foregoing description shows and describes several preferred embodiments of the invention. However, as previously stated, it should be understood that the invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the invention is applicable to various other combinations, modifications, and environments and is capable of modification within the scope of the inventive concept described herein, through the teachings above, or through techniques or knowledge in the relevant art. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the invention are intended to be within the scope of the appended claims.

Claims

1. A method for producing a pair of egg-laying green-shell Pu'an Panjiang black-bone chickens, comprising the following steps: 1) Paternal breeding: 101) Select the existing Zhuxiang Chicken ZX line, and breed males that meet the weight and appearance standards at 16 weeks and 43 weeks of age, and the hens that meet the weight and egg production standards at 16 weeks and 43 weeks of age, and the appearance standards. Then, select and breed the great-grandfather ZX paternal father and the great-grandfather ZX paternal mother according to the weight and appearance standards at 16 and 43 weeks of age for males and the weight and egg production standards at 16 weeks and 43 weeks of age for females; 102) The ZX paternal line father and the ZX paternal line mother of the great-grandfather generation are mated and selected according to the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, and the weight and egg production standards and appearance standards of hens at 16 weeks and 43 weeks of age, to obtain the ZX paternal line father and the ZX paternal line mother of the grandparent generation; 103) The grandparent ZX paternal line father and grandparent ZX paternal line mother were mated and selected based on the weight and appearance standards of roosters at 16 weeks and 43 weeks of age, the semen quality standards of roosters at 30 weeks of age, and the green-shell egg positive homozygous genotype to obtain the parent ZX paternal line father; 2) Maternal breeding: 201) Breeding roosters of the existing purebred Pu'an Panjiang black-bone chicken PA line that meet the weight and appearance standards at 16 weeks and 43 weeks of age with hens that meet the weight standards at 16 weeks and 43 weeks of age and the egg production and appearance standards at 43 weeks of age, and selecting and breeding according to the egg production and appearance standards of the hens at 43 weeks of age to obtain the great-grandfather generation PA maternal sire and the great-grandfather generation PA maternal dam; 202) The great-grandfather PA maternal sire is mated with the great-grandfather PA maternal sire and selected according to the egg production standard and appearance standard of the hen at 43 weeks of age to obtain the grandparent PA maternal sire; 203) Use the existing red-feathered black-tailed hen HH line that meets the appearance characteristics, egg production at 43 weeks of age, and weight at 16 and 43 weeks of age as the grandparent HH maternal line mother; 204) The grandparent generation PA maternal sire and the grandparent generation HH maternal dam are mated and the parent generation PH maternal sire is obtained by selecting the hen based on the egg production at 43 weeks of age and the weight and appearance standards at 16 and 43 weeks of age; 3) The ZX paternal parent obtained by paternal selection is crossed with the PH maternal parent obtained by maternal selection to obtain the egg-laying green-shell Pu'an Panjiang black-bone chicken.

2. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, wherein: In steps 101) to 103), the selection and breeding of the great-grandfather ZX paternal father, the great-grandfather ZX paternal mother, the grandparent ZX paternal father, the grandparent ZX paternal mother, and the parent ZX paternal father are all carried out by single cage breeding and artificial insemination.

3. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, wherein: In steps 201) to 204), the great-grandfather PA maternal parent, the great-grandfather PA maternal parent, the grandparent PA maternal parent, the grandparent HH maternal parent, and the parent PH maternal parent are all bred in single cages and hybridized by artificial insemination.

4. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: In steps 101) to 103), the weight standard of the Zhuxiang chicken rooster at 16 weeks of age is 1.75 kg, the weight standard at 43 weeks of age is 2.1 kg, the egg production standard at 43 weeks of age is that the same family hen lays more than 80 eggs at 43 weeks of age, the appearance characteristics of the Zhuxiang chicken rooster are strong and sturdy, the feathers have a dark green metallic luster, the sickle feathers are well developed, the feathers, black skin, black legs, black crown, and black meat are black, and the semen quality requirements of the Zhuxiang chicken rooster are a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, and fertilization rate 90% or more; the egg production standard of the Zhuxiang chicken hen at 43 weeks of age is an average egg production of more than 80 eggs with green eggshell color and an average egg weight of 50 g, the weight standard of the Zhuxiang chicken hen at 16 weeks of age is 1.3 kg, and the weight standard at 43 weeks of age is 1.8 kg, the appearance characteristics of the Bamboo Chicken are its sleek body, well-proportioned structure, black feathers, black skin, black legs, black crown and black meat.

5. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: In step 201) and step 202), the weight standard of the Pu'an Panjiang black-bone chicken rooster at 16 weeks of age is 1.4 kg, the weight standard at 43 weeks of age is 2.5 kg, the egg production standard at 43 weeks of age is that the average egg production of hens of the same family at 43 weeks of age is more than 65 eggs, the appearance standard of the Pu'an Panjiang black-bone chicken rooster is a strong physique, black feathers, black skin, black shanks, black crown, and black meat, the semen quality requirement of the Pu'an Panjiang black-bone chicken rooster is a single semen collection volume of more than 0.5 mL, milky white, thick, pH 7-8, density 3-5 billion / mL, vitality 0.7 or more, and fertilization rate more than 90%; the weight standard of the Pu'an Panjiang black-bone chicken hen at 16 weeks of age is 1.2 kg, the weight standard at 43 weeks of age is 1.9 kg, the egg production standard at 43 weeks of age is more than 65 eggs, the eggshell color is pink, and the average egg weight is 48. g. The appearance standard of the Pu'an Panjiang black-bone chicken hen is medium size, black feathers, black skin, black legs, black crown and black meat.

6. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: In step 203), the red-feathered, black-tailed hen HH has the appearance characteristics of a sleek body, red feathers, black tail, yellow shanks, a red single comb and white skin. The average weight at 16 weeks of age is 1.3 kg, the average weight at 43 weeks of age is 1.8 kg, the egg production at 43 weeks of age is more than 150 eggs, the average egg weight is 62.5 g, and the egg shell color is brown.

7. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: In step 204), the PH hen has black feathers and black skin, a standard weight of 1.2 kg at 16 weeks of age, a standard weight of 1.8 kg at 43 weeks of age, an egg production of 110 eggs at 43 weeks of age, an average egg weight of 55 g, and a light brown eggshell color.

8. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: In step 3), the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken has the appearance characteristics of "black feathers, black skin, black shanks, black crown, and black meat". The egg-laying green-shell Pu'an Panjiang black-bone chicken hens obtained have reduced egg production after 43 weeks of age and can be marketed as high-quality old hens; the obtained egg-laying green-shell Pu'an Panjiang black-bone chicken roosters can be marketed as high-quality broilers at 20-22 weeks of age.

9. The egg-laying green-shell Pu'an Panjiang black-bone chicken seed production method according to claim 1, characterized in that: Before mating, all breeder chickens in the matching system are purified for salmonella and chicken leukemia.

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