Preparation method of mildew-proof biological plasticized specimen based on silicone rubber

Abstract

The invention belongs to the technical field of biological plasticized specimens, and discloses a preparation method of a mildew-proof biological plasticized specimen based on silicone rubber. The method comprises the following steps: fixing a biological specimen by using formalin, sequentially bleaching, dehydrating and degreasing, and carrying out vacuum impregnation by using a mixed solution of silicon rubber, a catalyst and a mildew preventive as an impregnation solution; and after the vacuum infiltration is completed, curing in curing agent gas under a closed condition to obtain the mildew-proof biological plasticized specimen. According to the method, the mildew preventive is added into the mixed solution of the silicone rubber HOFFEN S10 and the catalyst HOFFEN S3, so that the plasticized specimen is not troubled by mildew any more, and meanwhile, secondary damage to the specimen caused by manual cleaning of the mildew is also avoided. In the aspect of teaching, a normal structure or a diseased region can be observed more clearly and accurately; in the aspect of exhibition, the phenomenon of wrong science popularization caused by unclear structure due to mould can be avoided.

Description

Technical Field

[0001] The invention relates to the technical field of biological plastination specimens, and in particular to a method for preparing a mildew-proof biological plastination specimen based on silicone rubber. Background Art

[0002] Silicone rubber technology is mainly used for the preparation of large-volume specimens. Using this method to preserve specimens can not only show the appearance of the specimens, but also preserve and display the internal structure of the organisms. The plasticized specimens are dry, odorless, can be preserved for a long time, and can preserve the original morphology of the cell structure. The development of this technology can enable biological specimens to be popularized and taught in their original form, which not only enriches biology teaching, but also enriches the display content of natural museums and science and technology museums. However, when specimens are stored in the air for a long time, the humidity and temperature in the environment will have a certain impact on the specimens. The mold produced on the specimens will cause unclear structures, thereby affecting the correctness of teaching and popularization. Moreover, the mold produced can only be removed manually to prevent secondary damage to the specimens. However, the appearance of mold not only consumes labor, but also affects the overall aesthetics of the specimens. Therefore, how to improve the mildew resistance of specimens is an urgent problem to be solved. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, so as to solve the problem that the existing specimens are prone to mold when stored in the air.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The present invention provides a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, comprising the following steps:

[0006] The biological specimen is fixed with formalin, and then bleached, dehydrated, and defatted in sequence to obtain a pretreated biological specimen;

[0007] vacuum infiltrating the pretreated biological specimen with an impregnation solution;

[0008] After the vacuum impregnation is completed, curing is carried out in a curing agent gas under a closed condition, thereby completing the preparation of the anti-mildew biological plastinated specimen based on silicone rubber;

[0009] The impregnation solution includes a silicone rubber solution, a catalyst solution and a mildewcide solution.

[0010] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the concentration of formalin is 5 to 20%; and the fixation time is 15 to 30 days.

[0011] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the solution used for dehydration is acetone; and the dehydration temperature is -25 to -15°C.

[0012] Preferably, in the above method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the solution used for degreasing is acetone; and the temperature for degreasing is 25°C.

[0013] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, in the immersion solution, the volume ratio of the silicone rubber solution, the catalyst solution and the mildew-proof agent solution is 97:2:1.

[0014] Preferably, in the above method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the silicone rubber solution is HOFFEN S10; the catalyst solution is HOFFEN S3; and the mildew-proof agent solution is a mixed solution of kasson and an inorganic salt stabilizer.

[0015] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the temperature of the vacuum impregnation is -25 to -15°C; the vacuum degree of the vacuum impregnation is -0.1 to 0 MPa; and the time of the vacuum impregnation is 12 to 24 hours.

[0016] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the curing agent gas is obtained by volatilizing a curing agent solution.

[0017] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the curing agent solution is HOFFEN S6.

[0018] Preferably, in the above-mentioned method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, the curing time is 7 to 15 days.

[0019] It can be seen from the above technical solution that compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention adds a mildew preventer to the mixed solution of silicone rubber HOFFEN S10 and catalyst HOFFEN S3, so that the plasticized specimen is no longer troubled by mildew, and also avoids the secondary damage to the specimen caused by manual cleaning of mildew. In teaching, the normal structure or the lesion site can be observed more clearly and accurately; in exhibition, it can avoid the phenomenon that the structure is unclear due to mildew, thus leading to erroneous popular science. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art are briefly introduced below.

[0022] Figure 1 This is the appearance of the biological plastination specimen of Comparative Example 1 after being stored for a period of time;

[0023] Figure 2 This is the appearance of the biological plastination specimen of Example 1 after being stored for a period of time. DETAILED DESCRIPTION

[0024] The present invention provides a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, comprising the following steps:

[0025] The biological specimen is fixed with formalin, and then bleached, dehydrated, and defatted in sequence to obtain a pretreated biological specimen;

[0026] vacuum infiltrating the pretreated biological specimen with an impregnation solution;

[0027] After the vacuum impregnation is completed, curing is carried out in a curing agent gas under closed conditions, thereby completing the preparation of the silicone rubber-based mildew-proof biological plastinated specimen.

[0028] In the present invention, the concentration of formalin is preferably 5-20%, more preferably 8-15%, and more preferably 10%; the fixation time is preferably 15-30 days, more preferably 15-20 days, and more preferably 15 days.

[0029] In the present invention, the bleaching solution is preferably hydrogen peroxide; the concentration of the hydrogen peroxide is preferably 5-10%, more preferably 8-10%, and more preferably 10%. The present invention does not limit the bleaching time, and the bleaching time can be adjusted according to the desired effect.

[0030] In the present invention, the solution used for dehydration is acetone; the dehydration temperature is preferably -25 to -15°C, more preferably -25 to -20°C, and more preferably -25°C; the dehydration time is until the water content in the acetone is ≤1%. In the present invention, the acetone is replaced every 2 to 3 days during the dehydration process.

[0031] In the present invention, the solution used for degreasing is acetone; the temperature for degreasing is preferably 25° C.; and the degreasing time is until the fat content in the acetone is ≤1%.

[0032] In the present invention, the impregnation solution includes a silicone rubber solution, a catalyst solution, and a mildewcide solution.

[0033] In the present invention, in the impregnation solution, the volume ratio of the silicone rubber solution, the catalyst solution, and the mildew-proofing agent solution is preferably 97:2:1.

[0034] In the present invention, the silicone rubber solution is preferably HOFFEN S10 produced by Dalian Hongfeng Biotechnology Co., Ltd.; the catalyst solution is preferably HOFFEN S3 produced by Dalian Hongfeng Biotechnology Co., Ltd.; and the mildew inhibitor solution is preferably a mixed solution of kason and an inorganic salt stabilizer.

[0035] In the present invention, the mass fraction of Kathon in the antifungal solution is preferably 14%; the mass fraction of the inorganic salt stabilizer in the antifungal solution is preferably 0.5-1%, more preferably 0.7-1%, more preferably 1%; the inorganic salt stabilizer is preferably magnesium nitrate.

[0036] In the present invention, the temperature of the vacuum impregnation is preferably -25 to -15°C, more preferably -22 to -17°C, and more preferably -20°C; the vacuum degree of the vacuum impregnation is preferably -0.1 to 0 MPa, more preferably -0.1 to -0.05 MPa, and more preferably -0.1 MPa; the time of the vacuum impregnation is preferably 12 to 24 h, more preferably 16 to 24 h, and more preferably 24 h.

[0037] In the present invention, the vacuum degree gradually increases during the vacuum impregnation process, specifically from 0 MPa to -0.08 MPa, maintained for 8 to 12 h, and then increased to -0.1 MPa, maintained for 8 to 12 h.

[0038] In the present invention, the equipment used for the vacuum impregnation is preferably a SWL-1125 vacuum box.

[0039] In the present invention, the curing agent gas is obtained by volatilizing the curing agent solution.

[0040] In the present invention, the curing agent solution is preferably HOFFEN S6 produced by Dalian Hongfeng Biotechnology Co., Ltd.

[0041] In the present invention, the curing time is preferably 7 to 15 days, more preferably 7 to 12 days, and more preferably 7 days.

[0042] In the present invention, the curing agent solution needs to be replenished once every seven days during the curing process.

[0043] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Example 1

[0045] This embodiment provides a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, comprising the following steps:

[0046] The biological specimen is fixed with 10% formalin for 15 days, then bleached with 10% hydrogen peroxide, the residual hydrogen peroxide is washed off after bleaching, and then dehydrated in acetone at -25°C, and the acetone is replaced every 2 days during the dehydration process until the water content in the acetone is ≤1% and the dehydration is completed; then, degreasing is performed in acetone at 25°C until the fat content in the acetone is ≤1% and the degreasing is completed, thereby obtaining a pretreated biological specimen;

[0047] The pretreated biological specimens were vacuum impregnated with an impregnation solution in a SWL-1125 vacuum box at -20°C. The impregnation solution was a mixture of silicone rubber solution HOFFEN S10, catalyst solution HOFFEN S3, and mildew inhibitor solution (14% by mass of kason and 1% by mass of magnesium nitrate) in a volume ratio of 97:2:1. During the impregnation process, the vacuum degree increased from 0MPa to -0.08MPa and was maintained for 12h, then increased to -0.1MPa and maintained for 12h. Acetone was used as a medium to boil and overflow, and bubbles appeared on the surface of the biological specimens. The impregnation was completed when no bubbles were generated. The total impregnation time was 24h.

[0048] After the vacuum impregnation is completed, the curing agent solution HOFFEN S6 is volatilized into curing agent gas in a closed space, and the specimen is cured in the curing agent gas for 7 days. During the curing process, the curing agent solution is replenished so that the closed space is filled with curing agent gas, thereby completing the preparation of the silicone rubber-based mildew-proof biological plastinated specimen.

[0049] Comparative Example 1

[0050] This comparative example provides a method for preparing a biological plastination specimen based on silicone rubber, which is specifically referred to in Example 1, except that there is no antifungal solution in the immersion solution, and the volume ratio of the silicone rubber solution HOFFEN S10 to the catalyst solution HOFFEN S3 is 97:3.

[0051] After the treated biological plastinates of Example 1 and Comparative Example 1 were stored under the same conditions for the same period of time, the appearance of the biological plastinates was as follows: Figure 1 and Figure 2 As shown. Figure 1 and Figure 2 It can be seen that mold appears on the surface of the biological plastinate specimens that have not been treated with anti-mold agents after being stored for a period of time, while mold is not observed on the biological plastinate specimens that have been treated with anti-mold agents, indicating that the treatment method of the present invention can effectively solve the problem of biological plastinate specimens being troubled by mold.

[0052] Example 2

[0053] This embodiment provides a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, comprising the following steps:

[0054] The biological specimen is fixed with 5% formalin for 20 days, then bleached with 5% hydrogen peroxide, the residual hydrogen peroxide is washed off after bleaching, and then dehydrated in acetone at -20°C, and the acetone is replaced once every 3 days during the dehydration process until the water content in the acetone is ≤1% and the dehydration is completed; then, degreasing is performed in acetone at 25°C until the fat content in the acetone is ≤1% and the degreasing is completed, thereby obtaining a pretreated biological specimen;

[0055] The pretreated biological specimens were vacuum impregnated with an impregnation solution in a SWL-1125 vacuum box at -25°C. The impregnation solution was a mixture of silicone rubber solution HOFFEN S10, catalyst solution HOFFEN S3, and mildew inhibitor solution (14% kason and 0.5% magnesium nitrate) in a volume ratio of 97:2:1. During the impregnation process, the vacuum degree increased from 0MPa to -0.08MPa, maintained for 10h, and then increased to -0.1MPa, maintained for 8h. Acetone was used as a medium to boil and overflow, and bubbles appeared on the surface of the biological specimens. The impregnation was completed when no bubbles were generated. The total impregnation time was 18h.

[0056] After the vacuum impregnation is completed, the curing agent solution HOFFEN S6 is volatilized into curing agent gas in a closed space, and the specimen is cured in the curing agent gas for 8 days. During the curing process, the curing agent solution is replenished so that the closed space is filled with curing agent gas, thereby completing the preparation of the silicone rubber-based mildew-proof biological plastinated specimen.

[0057] Example 3

[0058] This embodiment provides a method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, comprising the following steps:

[0059] The biological specimen is fixed with 20% formalin for 25 days, then bleached with 5% hydrogen peroxide, the residual hydrogen peroxide is washed off after bleaching, and then dehydrated in acetone at -15°C, and the acetone is replaced once every 2 days during the dehydration process until the water content in the acetone is ≤1% and the dehydration is completed; then, degreasing is performed in acetone at 25°C until the fat content in the acetone is ≤1% and the degreasing is completed, thereby obtaining a pretreated biological specimen;

[0060] The pretreated biological specimens were vacuum impregnated with an impregnation solution in a SWL-1125 vacuum box at -15°C. The impregnation solution was a mixture of silicone rubber solution HOFFEN S10, catalyst solution HOFFEN S3, and mildew inhibitor solution (14% kason and 0.7% magnesium nitrate) in a volume ratio of 97:2:1. During the impregnation process, the vacuum degree increased from 0MPa to -0.08MPa, maintained for 8h, and then increased to -0.1MPa, maintained for 12h. Acetone was used as a medium to boil and overflow, and bubbles appeared on the surface of the biological specimens. The impregnation was completed when no bubbles were generated. The total impregnation time was 20h.

[0061] After the vacuum impregnation is completed, the curing agent solution HOFFEN S6 is volatilized into curing agent gas in a closed space, and the specimen is cured in the curing agent gas for 15 days. During the curing process, the curing agent solution is replenished so that the closed space is filled with curing agent gas, thereby completing the preparation of the silicone rubber-based mildew-proof biological plastinated specimen.

[0062] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a mildew-proof biological plastinated specimen based on silicone rubber, characterized in that: The following steps are involved: The biological specimen is fixed with formalin, and then bleached, dehydrated, and defatted in sequence to obtain a pretreated biological specimen; vacuum infiltrating the pretreated biological specimen with an impregnation solution; After the vacuum impregnation is completed, curing is carried out in a curing agent gas under a closed condition, thereby completing the preparation of the anti-mildew biological plastinated specimen based on silicone rubber; The impregnation solution includes a silicone rubber solution, a catalyst solution and a mildewcide solution.

2. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 1, characterized in that: The concentration of the formalin is 5-20%; and the fixing time is 15-30 days.

3. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 2, characterized in that: The solution used for the dehydration is acetone; the temperature of the dehydration is -25 to -15°C.

4. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 3, characterized in that: The solution used for degreasing is acetone; the temperature of degreasing is 25°C.

5. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 1 or 4, characterized in that: In the impregnation solution, the volume ratio of the silicone rubber solution, the catalyst solution and the mildew-proofing agent solution is 97:2:

1.

6. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 5, characterized in that: The silicone rubber solution is HOFFEN S10; the catalyst solution is HOFFEN S3; and the mildew inhibitor solution is a mixed solution of kason and an inorganic salt stabilizer.

7. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 6, characterized in that: The temperature of the vacuum impregnation is -25 to -15°C; the vacuum degree of the vacuum impregnation is -0.1 to 0 MPa; and the time of the vacuum impregnation is 12 to 24 hours.

8. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 5, characterized in that: The curing agent gas is obtained by volatilizing the curing agent solution.

9. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 8, characterized in that: The curing agent solution is HOFFEN S6.

10. The method for preparing a mildew-proof biological plastinated specimen based on silicone rubber according to claim 7, characterized in that: The curing time is 7 to 15 days.

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