Growth-promoting plant growth regulator for forest trees as well as preparation method and application of growth-promoting plant growth regulator

By using a plant growth regulator containing components such as Huangcen fermentation, the problems of slow growth of Qinghai spruce in desert soil and difficulty in germination of seeds are solved, which significantly improves the growth rate and stress resistance of the plants.

CN119908372AActive Publication Date: 2025-05-02SHANDONG ZHONGXIN AGRI DEV CO LTD
View PDF 7 Cites 0 Cited by

Patent Information

Application Number
CN202510404844.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-02
Publication Date
2025-05-02
Estimated Expiration
2045-04-02

AI Technical Summary

Technical Problem

Qinghai spruce grows slowly in desert soil, and seed germination and seedling growth are affected by environmental conditions such as soil temperature and humidity, resulting in poor results in large-scale desertification control and afforestation.

Method used

A progenitor plant growth regulator for forest trees, including yellow fermented substances, agar oligosaccharides, salicylic acid, echinacea polysaccharides, indole butyrate and sodium naphthaleneacetate, is used to release biologically active substances through enzymatic lysis and fermentation treatment to promote plant growth and root development.

Benefits of technology

It significantly improves the growth rate of Qinghai spruce plants and the germination rate of seeds, enhances the stress resistance and root absorption capacity of seedlings, and solves the problem of Qinghai spruce growing slowly in desert soil.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN119908372A_ABST
    Figure CN119908372A_ABST
Patent Text Reader

Abstract

The invention discloses a growth-promoting plant growth regulator for forest trees as well as a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The plant growth regulator is prepared from the following raw materials in parts by weight: 5 to 7 parts of radix scutellariae fermentation products, 0.01 to 0.02 part of agaro-oligosaccharide, 4 to 5 parts of salicylic acid, 1 to 2 parts of echinacea polysaccharide, 0.2 to 0.3 part of indole-3-butyric acid potassium and 0.6 to 0.8 part of pimacol. The plant growth regulator disclosed by the invention is applied to picea crassifolia, and all the components in the regulator have a synergistic effect, so that the growth of picea crassifolia plants can be remarkably improved, and meanwhile, the germination rate of picea crassifolia seeds is increased. The plant growth regulator disclosed by the invention effectively solves the problems of slow growth and low wood yield of picea crassifolia, is safe and environment-friendly in components, and has a positive effect on reducing environmental pollution and pesticide residues.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically relates to a growth-promoting plant growth regulator for forest trees, a preparation method and an application thereof. Background Art

[0002] Picea crassifolia Picea crassifolia kom. ) Picea spruce, an evergreen tree, is mainly distributed in Qinghai, Gansu and other places. Compared with other tree species, Picea spruce grows slowly, but it has strong adaptability and low requirements for the temperature and humidity of the growing environment. It can still grow normally in a cold and humid environment. Picea spruce is tolerant to barrenness and can grow in neutral soil. It is often used in desert management, which has the functions of preventing wind and sand, preventing soil erosion, and improving the local ecological environment. Picea spruce has a straight and sturdy trunk, excellent material and is often used to make furniture. The seeds are edible with high nutritional value. Due to its beautiful tree posture, Picea spruce is also often used as an ornamental tree species. In short, Picea spruce has high ecological value, economic value and ornamental value. However, Picea spruce has small seeds, with a thousand-grain weight of only about 4.5g. It has high requirements for soil temperature and humidity during sowing. Cold, drought, compacted soil or high temperature, high humidity and waterlogging will affect the germination of seeds and the growth of seedlings.

[0003] Carrying out large-scale sand control and afforestation is one of the effective ways to improve land desertification. Planting Qinghai spruce on a large scale is of great significance to solving the problem of land desertification. However, how to accelerate the rapid growth of Qinghai spruce in desert soil is an issue that urgently needs to be explored.

[0004] In forestry production, the height and diameter growth of seedlings, the rooting of cuttings, and the survival rate of afforestation are all key issues affecting seedling and forest cultivation. Plant growth regulators play a certain metabolic role in plants, and achieve the purpose of strengthening seedlings by regulating plant growth and affecting the shift of plant growth center of gravity. Therefore, plant growth regulators play an important role in increasing agricultural and forestry production and income. Summary of the invention

[0005] The main purpose of the present invention is to provide a growth-promoting plant growth regulator for forest trees, which can significantly improve the growth of Picea crassifolia plants and simultaneously improve the germination rate of Picea crassifolia seeds.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 5-7 parts of Scutellaria baicalensis fermentation product, 0.01-0.02 parts of agar oligosaccharide, 4-5 parts of salicylic acid, 1-2 parts of Echinacea purpurea polysaccharide, 0.2-0.3 parts of potassium indolebutyrate, and 0.6-0.8 parts of sodium naphthylacetate.

[0007] Furthermore, the preparation method of the Scutellaria baicalensis fermented product is: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0008] Furthermore, in step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0009] Furthermore, the composite hydrolase solution contains 30-40 g / L of β-glucosidase and 12-18 g / L of hemicellulase.

[0010] Furthermore, in step S2, the added amount of Bacillus niabensis is 10-15 g / L, and the added amount of Acinetobacter berrezii is 15-25 g / L.

[0011] Furthermore, the number of live bacteria of Bacillus niabensis is 1 billion / gram, and the number of live bacteria of Acinetobacter berrezii is 1 billion / gram.

[0012] Furthermore, the strain number of Bacillus niabensis in step S2 is CGMCC1.16140, purchased from China General Microbiological Culture Collection Administration Center, and the original preservation date is March 20, 2017; the strain number of Acinetobacter berrezii is CGMCC1.12683, purchased from China General Microbiological Culture Collection Administration Center, and the original preservation date is September 15, 2013.

[0013] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0014] The invention discloses an application of a plant growth regulator in promoting forest growth.

[0015] Furthermore, the trees are Picea crassifolia.

[0016] The raw materials used in the present invention are all commercially available.

[0017] The regulator of the present invention adopts the fermentation product of Scutellaria baicalensis, which contains a variety of biologically active substances. In the present invention, the cell wall of Scutellaria baicalensis is destroyed by enzymatic hydrolysis to release more active ingredients; in addition, Bacillus niabensis and Acinetobacter berrezi produce metabolites in the fermentation process, which have the functions of dissolving phosphorus and secreting the growth hormone indoleacetic acid (IAA), thereby promoting plant growth.

[0018] The present invention adds Echinacea polysaccharide, which can provide carbon source and energy supply for plants, can regulate the synthesis of hormones in plants, and thus regulate the growth rhythm of plants; in addition, it plays an important role in the nutrient absorption of plants, and can form an adhesion layer on the surface of the root system, increase the contact area between the root system and soil particles, and improve the absorption efficiency of nutrients; in addition, it can also form complexes with some mineral elements, increase the availability of these elements in the soil, and promote the absorption of mineral elements by plants. The present invention adds agar oligosaccharide, which stimulates plant growth to a certain extent and improves the stress resistance of plants.

[0019] Beneficial Effects The components in the regulator of the present invention work synergistically, on the one hand, promoting rooting, improving the absorption of nutrients by the root system, and accelerating the cell division rate of the plant to promote the growth of the plant; on the other hand, by adjusting the water balance of the plant, increasing the drought tolerance of the cell, and improving the resistance of the plant to harsh environments such as drought; in addition, it can increase the germination rate of Qinghai spruce seeds. The plant growth regulator of the present invention can effectively solve the problems of slow growth and low wood yield of Qinghai spruce, and the components are safe and environmentally friendly, and have a positive effect on reducing environmental pollution and reducing agricultural residues. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 These are diagrams showing the phosphorus solubilization effects of Bacillus niabensis and Acinetobacter berezzi. Note: Figure A shows Bacillus niabensis and Figure B shows Acinetobacter berezzi. DETAILED DESCRIPTION

[0021] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0022] Example 1 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 5 parts of Scutellaria baicalensis fermentation product, 0.01 parts of agar oligosaccharide, 4 parts of salicylic acid, 1 part of Echinacea purpurea polysaccharide, 0.2 parts of potassium indolebutyrate, and 0.6 parts of sodium naphthylacetate.

[0023] The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0024] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0025] The composite hydrolase solution contains 30 g / L of β-glucosidase and 12 g / L of hemicellulase.

[0026] In the step S2, the added amount of Bacillus niabensis is 10 g / L and the added amount of Acinetobacter berreitzii is 15 g / L.

[0027] The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

[0028] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0029] Example 2 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 6 parts of Scutellaria baicalensis fermentation product, 0.01 parts of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of Echinacea purpurea polysaccharide, 0.2 parts of potassium indolebutyrate, and 0.7 parts of sodium naphthylacetate.

[0030] The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0031] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0032] The composite hydrolase solution contains 35 g / L of β-glucosidase and 15 g / L of hemicellulase.

[0033] In the step S2, the added amount of Bacillus niabensis is 12 g / L and the added amount of Acinetobacter berreitzii is 20 g / L.

[0034] The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

[0035] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0036] Example 3 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 7 parts of Scutellaria baicalensis fermentation product, 0.02 parts of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of Echinacea purpurea polysaccharide, 0.3 parts of potassium indolebutyrate, and 0.8 parts of sodium naphthylacetate.

[0037] The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0038] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0039] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0040] In the step S2, the added amount of Bacillus niabensis is 15 g / L and the added amount of Acinetobacter berreitzii is 25 g / L.

[0041] The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

[0042] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0043] Comparative Example 1 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 7 parts of Scutellaria baicalensis fermentation product, 0.02 parts of manno-oligosaccharide, 5 parts of salicylic acid, 2 parts of Echinacea purpurea polysaccharide, 0.3 parts of potassium indolebutyrate, and 0.8 parts of sodium naphthylacetate.

[0044] The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0045] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0046] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0047] In the step S2, the added amount of Bacillus niabensis is 15 g / L and the added amount of Acinetobacter berreitzii is 25 g / L.

[0048] The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

[0049] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, mannooligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0050] Compared with Example 3, this comparative example is the same as Example 3 except that agar oligosaccharide is replaced by manno oligosaccharide.

[0051] Comparative Example 2 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 7 parts of Scutellaria baicalensis fermentation product, 0.02 parts of agar oligosaccharide, 5 parts of salicylic acid, 0.3 parts of potassium indolebutyrate, and 0.8 parts of sodium naphthylacetate.

[0052] The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50° C. for 2-4 hours, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for later use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

[0053] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0054] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0055] In the step S2, the added amount of Bacillus niabensis is 15 g / L and the added amount of Acinetobacter berreitzii is 25 g / L.

[0056] The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

[0057] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0058] Compared with Example 3, this comparative example is the same as Example 3 except that Echinacea polysaccharide is not added. The other raw materials and steps are the same as those of Example 3.

[0059] Comparative Example 3 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 7 parts of Scutellaria baicalensis extract, 0.02 parts of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of Echinacea purpurea polysaccharide, 0.3 parts of potassium indolebutyrate, and 0.8 parts of sodium naphthylacetate.

[0060] The preparation method of the Scutellaria baicalensis extract is as follows: The root of Scutellaria baicalensis is dried and crushed to obtain Scutellaria baicalensis powder, and a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50°C for 2-4 hours, and the mixture is inactivated at high temperature, cooled to room temperature, centrifuged, and the supernatant is taken and dried to obtain the Scutellaria baicalensis extract.

[0061] In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

[0062] The composite hydrolase solution contains 40 g / L of β-glucosidase and 18 g / L of hemicellulase.

[0063] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The scutellaria baicalensis extract, agar oligosaccharide, salicylic acid, echinacea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

[0064] Compared with Example 3, this comparative example has the same raw materials and steps as Example 3, except that the Scutellaria baicalensis is not fermented.

[0065] Comparative Example 4 A growth-promoting plant growth regulator for forest trees, comprising the following raw materials in parts by weight: 0.02 parts of agar oligosaccharide, 5 parts of salicylic acid, 2 parts of Echinacea polysaccharide, 0.3 parts of potassium indolebutyrate, and 0.8 parts of sodium naphthylacetate.

[0066] A method for preparing a growth-promoting plant growth regulator for forest trees, comprising the following steps: The plant growth regulator is obtained by uniformly mixing agar oligosaccharide, salicylic acid, Echinacea polysaccharide, potassium indolebutyrate and sodium naphthylacetate.

[0067] Compared with Example 3, this comparative example has the same raw materials and steps as Example 3 except that the fermented material of Scutellaria baicalensis is not added.

[0068] Performance Testing Determination of strain-related metabolites Phosphorus dissolving capacity determination: (1) Strain activation: Thaw Bacillus niabensis and Acinetobacter berrezii and transfer them into LB medium and place them at 30°C for activation culture for 24-48 hours to obtain activated strains of the two bacteria.

[0069] (2) The strain was inoculated onto an inorganic phosphorus solid culture medium and cultured in a constant temperature incubator at 30°C for 3 days to observe the formation of a phosphate-dissolving zone.

[0070] Determination of the ability to produce indoleacetic acid (IAA): The IAA biosynthesis capacity of the strain was determined by the Salkowski method. First, the strain was inoculated in a solution containing 200 mg·L - 1 The culture medium was incubated in a shaking platform at 30°C and 170 r / min for 3 days in a L-tryptophan NA liquid medium. The culture medium was centrifuged at 4°C and 10,000 r / min for 10 min using a low-temperature centrifuge. The supernatant was removed and an equal volume of Salkowski colorimetric solution was added. After standing for 30 min in the dark, the absorbance was measured at a wavelength of 530 nm using an ultraviolet spectrophotometer to determine the IAA synthesis capacity of the strain. The data statistics are shown in Table 1.

[0071] Table 1 Determination of indoleacetic acid production capacity

[0072] Planting test Experimental Materials and Methods Experiment 1: The growth-promoting effect of the plant growth regulator of the present invention on Picea crassifolia plants.

[0073] The test Qinghai spruce seedlings were uniform in growth state and about 50 cm in height and were purchased from a local nursery stock market. The test was conducted in Haiguinan County, Qinghai Province. The test Qinghai spruce seedlings were randomly divided into 8 treatment groups: a control group (blank control) and 7 test groups using the plant growth regulators obtained in Examples 1-3 and Comparative Examples 1-4, with 20 plants in each treatment group, divided into 4 replicate groups.

[0074] The randomly divided spruce seedlings were planted in sand treated with carbendazim wettable powder, with a row spacing of 3m×2m, a spruce seedling planting pit specification of 40cm×35cm, and a planting depth of 25cm, and unified management measures were taken for all spruces. The experiment started on March 1, 2023, and the leaves were sprayed with the regulators of Examples 1-3 and Comparative Examples 1-4 diluted 300 times every six weeks or so. Windless weather was selected for spraying, and each spraying was carried out until water dripped on both sides of the leaves. A total of 3 spraying treatments were carried out in mid-April, early June, and mid-July, respectively. The new shoot growth, crown growth, ground diameter, new root growth, and lateral root growth of the seedlings were counted in September 2023, and the data were recorded in Table 2.

[0075] Table 2 Growth status of Picea crassifolia

[0076] As shown in Table 2, compared with the control group, the plant growth regulators of Examples 1-3 of the present invention can significantly increase the growth of new shoots, crown width, new root growth and lateral root growth of Picea hainanensis, indicating that the plant growth regulators of the present invention have a significant growth-promoting effect on Picea hainanensis plants. However, the growth-promoting effect of Comparative Examples 1-4, which change the components of the regulator, on Picea hainanensis is weakened to varying degrees. Therefore, the components of the plant growth regulator of the present invention cooperate to promote the growth of Picea hainanensis, and none of them can be missing.

[0077] Experiment 2: Effect of the plant growth regulator of the present invention on the germination rate and seedling growth of Picea crassifolia seeds In early April of the following year after the seeds were harvested, seed germination tests and seedling growth measurements were conducted. The Qinghai spruce seed test set up eight treatment groups: a control group (blank control) and a test group that used the plant growth regulators of Examples 1-3 and Comparative Examples 1-4 for seed dressing. The amount of the plant growth regulator used was 3% of the weight of the Qinghai spruce seeds. 500 seeds were randomly selected from each of the eight treatment groups and sown in the seedbeds preset in the test site. The seed sowing density was 100 seeds / m 2 The emergence of seeds from the soil was considered as germination. The germination rate was calculated two months after sowing. Germination rate (%) = (number of seedlings emerging from the soil / number of seeds sown) × 100. 20 seedlings of Picea crassifolia were selected from each group 120 days after sowing to measure seedling height and root length.

[0078] Table 3 Performance of Picea crassifolia seeds under different treatments

[0079] As can be seen from the data in Table 3, the plant growth regulator of the present invention can significantly increase the germination rate of Picea crassifolia seeds, and increase the seedling height and root length of Picea crassifolia seedlings, indicating that the regulator of the present invention can increase the biomass of the seedlings. Therefore, the plant growth regulator of the present invention has a significant growth-promoting effect on Picea crassifolia seeds and seedlings.

[0080] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A plant growth regulator for forest trees, characterized in that: The method comprises the following raw materials in parts by weight: 5-7 parts of Scutellaria baicalensis fermentation product, 0.01-0.02 parts of agar oligosaccharide, 4-5 parts of salicylic acid, 1-2 parts of Echinacea purpurea polysaccharide, 0.2-0.3 parts of potassium indolebutyrate, and 0.6-0.8 parts of sodium naphthylacetate.

2. The forest growth promoting plant growth regulator according to claim 1, characterized in that: The preparation method of the Scutellaria baicalensis fermented product is as follows: S1: After drying and crushing the root of Radix Scutellariae to obtain Radix Scutellariae powder, a composite hydrolase solution containing β-glucosidase and hemicellulase is added, and enzymolysis is performed at 40-50°C for 2-4h, and the mixture is inactivated at high temperature and cooled to room temperature, and the mixture is used for standby use; S2: Add Bacillus niabensis and Acinetobacter berrezii to the mixed solution, ferment at 30°C for 24 hours, filter, centrifuge the fermentation solution, take the supernatant, and dry it to obtain the Scutellaria baicalensis fermentation product.

3. The forest growth promoting plant growth regulator according to claim 2, characterized in that: In the step S1, the mass-volume ratio of the Scutellaria baicalensis powder to the composite hydrolase solution is 1 kg:2 L.

4. The forest growth promoting plant growth regulator according to claim 3, characterized in that: The composite hydrolase solution contains 30-40 g / L of beta-glucosidase and 12-18 g / L of hemicellulase.

5. The forest growth promoting plant growth regulator according to claim 2, characterized in that: In the step S2, the added amount of Bacillus niabensis is 10-15 g / L, and the added amount of Acinetobacter berrezii is 15-25 g / L.

6. The forest growth promoting plant growth regulator according to claim 5, characterized in that: The viable count of the Bacillus niabensis is 1 billion / gram, and the viable count of Acinetobacter berrezii is 1 billion / gram.

7. The forest growth promoting plant growth regulator according to claim 2, characterized in that: The strain number of Bacillus niabensis in step S2 is CGMCC1.16140, which was purchased from China General Microbiological Culture Collection Administration Center; the strain number of Acinetobacter berrezii is CGMCC1.12683, which was purchased from China General Microbiological Culture Collection Administration Center.

8. A method for preparing the forest growth-promoting plant growth regulator according to any one of claims 1 to 7, characterized in that: Prepared by the following steps: The fermented product of Scutellaria baicalensis, agar oligosaccharide, salicylic acid, Echinacea purpurea polysaccharide, potassium indolebutyrate and sodium naphthylacetate are uniformly mixed to obtain a plant growth regulator.

9. Use of the forest tree growth-promoting plant growth regulator according to any one of claims 1 to 7 in promoting forest tree growth.

10. The use according to claim 9, characterized in that: The trees mentioned are Picea crassifolia.

Citation Information

Patent Citations

  • Composition capable of promoting rooting survival of plantlets

    CN105409954A

  • Traditional Chinese medicine fermentation organic slow-release fertilizer for late rice planting and preparation method thereof

    CN107021813A

  • Nutrient absorption promoter for activating trunk epidermis cells

    CN111995458A

  • Saline-alkali soil conditioner

    CN113429977A

  • Composite biological agent for phellinus igniarius planting and high-yield phellinus igniarius planting method

    CN116420741A