Composition for coloring animal tissues and application thereof

Abstract

The invention relates to the technical field of feed additives, in particular to a composition for coloring animal tissues and application of the composition. The invention provides application of a combination of one or more of dehydroxanthophyll, dehydrated xanthophyll and ethyl xanthophyll in coloring of animal tissues. Compared with a common colorant, the composition formed by the dehydroxanthophyll and the dehydrated xanthophyll or the dehydroxanthophyll, the dehydrated xanthophyll and the ethyl xanthophyll is used for feeding animals, so that a better animal tissue coloring effect can be generated, and the composition has important significance on reducing the dosage of the colorant and improving the animal tissue coloring effect and has a relatively good application value.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to a composition for animal tissue coloring and application thereof. Background Art

[0002] As people's living standards continue to improve, animal products with bright colors are becoming more and more popular. The color of animal products directly affects their economic value. Taking poultry as an example, in recent years, consumers have been particularly fond of "three yellow" (yellow feathers, yellow beaks, and yellow tarsus) broilers with beautiful colors. The coloring of "three yellow" chickens is one of the main sensory indicators for measuring their product quality, which directly affects their market demand and price.

[0003] Animals cannot synthesize pigments by themselves, so they must absorb and deposit them from feed. It is often difficult to meet the coloring requirements by relying solely on natural pigments from feed raw materials. Therefore, colorants are usually added to feed to improve the aesthetics and commercial value of animal products. The prior art discloses that lutein, zeaxanthin, canthaxanthin, etc. are added to feed as colorants to improve the color of products such as skin, fat, and yolk of broilers. However, with the increasing requirements of people for the color of three-yellow chickens, the coloring effects of lutein, zeaxanthin, canthaxanthin, etc. as colorants can no longer be met, and it is necessary to find suitable colorants to further improve the coloring effect. Therefore, the development of more efficient colorants is of great significance for improving the coloring effect of animal skin and skin derivatives and improving the quality of animal products. Summary of the invention

[0004] The invention provides a composition for animal tissue coloring and application thereof.

[0005] The present invention unexpectedly discovered in the process of developing a new colorant for animal tissue coloring that dehydrogenated lutein, dehydrated lutein and ethyl lutein can be used alone or in different compositions thereof to color animal tissues, especially when used in combination, dehydrogenated lutein and dehydrated lutein are compounded in a specific ratio or dehydrogenated lutein, dehydrated lutein and ethyl lutein are compounded in a specific ratio to produce a synergistic effect on the coloring effect of the tarsus, beak and underwing skin of three-yellow chickens, and the coloring effect is significantly better than the coloring effect of the above components used alone at the same dosage, and the coloring effect is also significantly better than commonly used colorants such as lutein and zeaxanthin.

[0006] Specifically, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides the use of a combination of one or more selected from dehydrogenated lutein, anhydrolutein, and ethyl lutein in coloring animal tissues or improving the color of animal tissues.

[0008] In the present invention, the animals include non-human mammals and birds, preferably birds.

[0009] In some embodiments of the present invention, the animal is a chicken, a duck, a goose, a quail or a pigeon, preferably a chicken.

[0010] In the present invention, the animal tissue includes animal tarsus, subwing skin or beak, etc.

[0011] The above application includes feeding one or more combinations selected from dehydrogenated lutein, dehydrated lutein, and ethyl lutein to animals. By feeding one or more combinations selected from dehydrogenated lutein, dehydrated lutein, and ethyl lutein to animals, the skin or skin derivatives of the animals can be colored.

[0012] In the present invention, the dehydrated lutein is dehydrated lutein I, and the chemical formula is C 40 H 54 O. Dehydrolutein is 3'-dehydrolutein, with the chemical formula C 40 H 54 O2. Ethyl lutein is 3'-ethyl lutein, English name 3'-ethyl lutein ether, chemical formula is C 42 H 60 O2.

[0013] Both dehydrated lutein I and 3'-dehydrolutein can be purchased from commercial sources. 3'-Ethyl lutein has been disclosed in the literature "Chi J. Oxidation of Zeaxanthin and characterization of 3'-Alkyl Luteinethers [J]. 2001", and can be prepared using known methods or purchased from commercial sources.

[0014] Preferably, the combination of multiple types of the above-mentioned dehydrogenated lutein, dehydrated lutein and ethyl lutein includes: dehydrogenated lutein and dehydrated lutein, or dehydrogenated lutein, dehydrated lutein and ethyl lutein.

[0015] As an embodiment, the above application includes feeding dehydrogenated lutein and dehydrated lutein to animals, wherein the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-20):1.

[0016] Animal experiments have shown that when dehydrogenated lutein and dehydrated lutein are mixed in the above mass ratio and fed to animals, the animal tissues can produce a better coloring effect, especially the tarsus and plantars, and a synergistic effect can be produced. That is, at the same feeding amount, the coloring effect of the dehydrogenated lutein and dehydrated lutein in the above ratio is significantly better than the coloring effect of either one fed separately.

[0017] Preferably, the above application comprises feeding dehydrogenated lutein and dehydrated lutein to animals, wherein the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-15):1.

[0018] As another embodiment, the above application includes feeding dehydrogenated lutein, dehydrated lutein and ethyl lutein to animals, wherein the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-20):1, and the mass ratio of dehydrogenated lutein to ethyl lutein is (2-5):1.

[0019] Animal experiments have shown that when dehydrogenated lutein, anhydrous lutein and ethyl lutein are mixed in the above mass ratio and fed to animals, the animal tissues can produce better coloring effects, and can also produce a synergistic effect in the coloring of tissues such as the tarsus, beak and underwing skin. That is, at the same feeding amount, the coloring effect of the above-mentioned ratio of dehydrogenated lutein, anhydrous lutein and ethyl lutein is significantly better than the coloring effect of the three fed separately.

[0020] In a second aspect, the present invention provides a composition for animal tissue coloring, the composition comprising dehydrogenated lutein and dehydrated lutein in a mass ratio of (1-20):1.

[0021] Preferably, in the composition, the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-15):1.

[0022] Preferably, the dehydrated lutein is dehydrated lutein I, and the dehydrogenated lutein is 3'-dehydrogenated lutein.

[0023] In some embodiments of the present invention, the coloring active ingredient of the composition consists only of dehydrolutein and dehydrated lutein in a mass ratio of (1-20):1.

[0024] In some other embodiments of the present invention, the composition further comprises ethyl lutein; in the composition, the mass ratio of dehydrolutein to ethyl lutein is (2-5):1.

[0025] Preferably, the ethyl lutein is 3'-ethyl lutein.

[0026] In some embodiments of the present invention, the composition comprises dehydrogenated lutein, anhydrogenated lutein and ethyl lutein, wherein the mass ratio of dehydrogenated lutein to anhydrogenated lutein is (1-15):1, and the mass ratio of dehydrogenated lutein to ethyl lutein is (2-5):1.

[0027] The composition can be used as a colorant for feeding animals to achieve coloring of animal tissues. Compared with commonly used colorants (such as lutein, zeaxanthin, etc.), the composition can achieve better coloring effects at significantly lower feeding amounts, which is of great significance in reducing the amount and cost of colorants added and improving coloring effects.

[0028] Preferably, the composition comprises dehydrogenated lutein, dehydrated lutein and ethyl lutein, wherein the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-3):1, and the mass ratio of dehydrogenated lutein to ethyl lutein is (2-5):1.

[0029] The above composition may consist of the above components alone, or may be further added with other colorants for use in combination.

[0030] In a third aspect, the present invention provides use of the above-mentioned composition in coloring animal tissue or improving the chromaticity of animal tissue.

[0031] In a fourth aspect, the present invention provides use of the above-mentioned composition in preparing a product having an animal tissue coloring function.

[0032] In the above application, the animal is preferably poultry, more preferably chicken, duck, goose, quail or pigeon, and most preferably chicken.

[0033] The tissue is preferably skin or a skin derivative; more preferably beak, tarsus or underwing skin, most preferably tarsus. Some compositions of the present invention are particularly suitable for improving the coloring effect of tarsus of poultry.

[0034] In a fifth aspect, the present invention provides use of the above-mentioned composition in the preparation of feed or feed additives.

[0035] The composition can be used as a colorant in feed or feed additives.

[0036] In a sixth aspect, the present invention provides a product comprising the composition described above.

[0037] The above product can be feed or feed additive.

[0038] The feed may be compound feed, oil feed, vitamin feed, protein feed, energy (carbohydrate) feed, etc.

[0039] In addition to the above composition, the feed may also contain one or more selected from energy substances, proteins, oils, vitamins and minerals.

[0040] In a seventh aspect, the present invention provides a method for coloring animal tissues, the method comprising: feeding an animal with the composition or product described above.

[0041] In an eighth aspect, the present invention provides a method for producing poultry with yellow skin or skin derivatives, the method comprising: feeding the animal with the composition or product described above.

[0042] Preferably, in the above method, the poultry is three-yellow chicken.

[0043] The beneficial effects of the present invention include at least: the present invention provides a novel colorant composition for animal tissue coloring, in which each component can produce a synergistic effect under a specific ratio, significantly improving the coloring effect of the composition. Compared with commonly used colorants (lutein, zeaxanthin, etc.), feeding animals with the colorant composition can produce a better animal tissue coloring effect with a smaller feeding amount, which is of great significance for reducing the amount of colorant used and improving the animal tissue coloring effect, and has good application value. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] The composition for animal tissue coloring provided by the present invention comprises dehydrogenated lutein and dehydrated lutein in a mass ratio of (1-20):1.

[0046] Preferably, in the composition, the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-15):1.

[0047] As an embodiment of the present invention, the coloring active ingredient of the composition consists of dehydrogenated lutein and dehydrated lutein in a mass ratio of (1-20):1.

[0048] As another embodiment of the present invention, the composition further comprises ethyl lutein; in the composition, the mass ratio of dehydrogenated lutein to ethyl lutein is (2-5):1, and the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-20):1.

[0049] The dehydrogenated lutein used in the following examples is 3'-dehydrogenated lutein, and the dehydrated lutein is dehydrated lutein I, both of which can be purchased from commercial sources. The ethyl lutein used is 3'-ethyl lutein, which can be prepared by the following method:

[0050] 1. Solution preparation: Add 100 μL of concentrated hydrochloric acid to 10 mL of anhydrous ethanol, mix thoroughly to obtain acidic ethanol, and place in a 4°C refrigerator for later use.

[0051] 2. Sample preparation: Weigh 0.1 mg of lutein crystals and slowly add acidic ethanol (about 5 mL) to it until the lutein crystals are completely dissolved. Place the mixture in a refrigerator overnight for 24 hours to ensure that the reaction is complete; then, add 10 mL of deionized water to the mixture to quench the reaction, and then add 10 mL of saturated sodium bicarbonate solution to neutralize the reaction product to obtain a mixture containing ethyl lutein.

[0052] 3. Separation and purification: Add 100 mL of dichloromethane to the mixed solution obtained in step 2 for liquid-liquid extraction. After sufficient shaking, stand and separate the layers, and collect the dichloromethane layer; repeat the extraction three times, combine the three dichloromethane layers; vacuum concentrate and dry in a cold water bath, and the resulting powder is 3'-ethyl lutein.

[0053] The dehydrolutein, dehydrated lutein and ethyl lutein in the composition of the present invention can be detected by high performance liquid chromatography, and the detection conditions are as follows:

[0054] Mobile phase: n-hexane: acetone = 81:19, isocratic elution is used for analysis; during the elution process, the flow rate is controlled at 1.1 mL / min; column temperature: 28°C; chromatographic column: Kromasil 100-5SIL (150×4.6 mm, particle size 5 μm); UV detector wavelength: 473 nm; injection volume: 20 μL. The retention time of dehydrated lutein is about 3.6-3.9 min, the retention time of dehydrogenated lutein is about 3.2-3.6 min, and the retention time of ethyl lutein is about 3.9-4.3 min. The content of each component in the composition is calculated by the area normalization method, and the percentage of the peak area of ​​each component to the total peak area is the content ratio of each component in the composition.

[0055] Example 1

[0056] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein and dehydrated lutein in a mass ratio of 2.5:1.

[0057] Example 2

[0058] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein and dehydrated lutein in a mass ratio of 15:1.

[0059] Example 3

[0060] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein and dehydrated lutein in a mass ratio of 10:1.

[0061] Example 4

[0062] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein and dehydrated lutein in a mass ratio of 5:1.

[0063] Example 5

[0064] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein and dehydrated lutein in a mass ratio of 1:1.

[0065] Example 6

[0066] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein, dehydrated lutein and ethyl lutein in a mass ratio of 5:5:1.

[0067] Example 7

[0068] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein, dehydrated lutein and ethyl lutein in a mass ratio of 2.5:1:1.

[0069] Example 8

[0070] This embodiment provides a composition for animal tissue coloring, which includes dehydrogenated lutein, dehydrated lutein and ethyl lutein in a mass ratio of 2:1:1.

[0071] Example 9

[0072] This embodiment provides a colorant, which is dehydrolutein.

[0073] Example 10

[0074] This embodiment provides a colorant, which is dehydrated lutein.

[0075] Embodiment 11

[0076] This embodiment provides a colorant, which is ethyl lutein.

[0077] Comparative Example 1

[0078] This comparative example provides a colorant, which is lutein.

[0079] Comparative Example 2

[0080] This comparative example provides a colorant, which is zeaxanthin.

[0081] Comparative Example 3

[0082] This comparative example provides a colorant, which is epoxy lutein.

[0083] Comparative Example 4

[0084] This comparative example provides a colorant composition, which is lutein and zeaxanthin in a mass ratio of 1:1.

[0085] Experimental example Animal experiment verifies the coloring effect

[0086] Yellow-feathered broilers were used as experimental animals to detect the coloring effects of the compositions or colorants of various embodiments and comparative examples on animal tissues, as follows:

[0087] Blank Group

[0088] A total of 48 60-day-old female yellow-feathered broilers (three-yellow chickens, initial body weight 1279±81g) were used. No colorant was added to the blank group feed. There were 6 replicates in the blank group, with 8 chickens in each replicate. The test days were 32 days, including 4 days of adaptation period and 28 days of test period. After the 28-day test period, 3 chickens were randomly selected from each replicate, and the yellowness values ​​of the tarsus and beak of the chickens were measured using a colorimetric fan, and the yellowness value of the skin under the wing area on the side of the chicken was measured using a colorimeter.

[0089] Experimental Group

[0090] 60-day-old female yellow-feathered broilers (three yellow chickens, initial body weight 1279 ± 81g) were used to set test groups 1-16, wherein test groups 1-11 were fed with the composition or colorant of Examples 1-11, respectively, and the composition or colorant was added in the feed in an amount of 0.2%, test groups 12-15 were fed with the composition or colorant of Comparative Examples 1-4, respectively, and the composition or colorant was added in the feed in an amount of 0.2%, and test group 16 was fed with the composition of Comparative Example 4, and the composition or colorant was added in the feed in an amount of 0.4%, and each test group was set with 6 repetitions, each with 8 chickens, and the test days were 32 days, wherein the adaptation period was 4 days and the test period was 28 days. After the end of the test period of 28 days, 3 chickens were randomly selected from each repetition of each group, and the yellowness values ​​of the tarsus and beak of the chicken were measured respectively with a colorimetric fan, and the yellowness value of the skin under the wing area on the side of the chicken was measured with a colorimeter.

[0091] The test results of each group of chickens were averaged, and the results are shown in Table 1. The results show that the coloring effects of the compositions of Examples 1-8 on the tarsus, beaks and underwing skin of yellow-feathered broilers are basically significantly better than the commonly used colorants (Comparative Examples 1, 2, 4) and the colorant composition of Comparative Example 4 with twice the dosage. Compared with dehydrated lutein, dehydrogenated lutein and ethyl lutein, the coloring effect of another lutein derivative, epoxy lutein, is poor (Comparative Example 3). Dehydrogenated lutein and dehydrogenated lutein are used in combination in a certain proportion (Examples 1-5), and the two can produce a synergistic effect in the coloring of the tarsus, and the coloring effect of the combination is significantly better than the coloring effect of the two used alone. Dehydrogenated lutein, dehydrogenated lutein and ethyl lutein are used in combination (Examples 6-8), and the three can produce a synergistic effect in the coloring of the tarsus, beak and underwing skin, and the coloring effect of the combination is significantly better than the coloring effect of the three used alone.

[0092] Table 1 Coloration of the beak, tarsus and underwing skin of chickens in the blank group and the experimental group

[0093]

[0094]

[0095] Note: a represents the difference between red and green. The higher the a value, the higher the redness and the better the flesh color.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Use of one or more combinations selected from dehydrogenated lutein, dehydrated lutein and ethyl lutein in coloring animal tissues or improving the chromaticity of animal tissues.

2. The use according to claim 1, characterized in that: The application comprises feeding an animal with a combination of one or more selected from dehydrogenated lutein, dehydrated lutein, and ethyl lutein; Preferably, the dehydrated lutein is dehydrated lutein I, the dehydrolutein is 3'-dehydrolutein, and the ethyl lutein is 3'-ethyl lutein.

3. The use according to claim 1 or 2, characterized in that: The application comprises feeding dehydrogenated lutein and dehydrated lutein to animals, wherein the mass ratio of dehydrogenated lutein to dehydrated lutein is (1-20):1; Alternatively, the application comprises feeding dehydrogenated lutein, anhydrogenated lutein and ethyl lutein to animals, wherein the mass ratio of dehydrogenated lutein to anhydrogenated lutein is (1-20):1, and the mass ratio of dehydrogenated lutein to ethyl lutein is (2-5):

1.

4. A composition for animal tissue coloring, characterized in that: The composition comprises dehydrogenated lutein and dehydrated lutein in a mass ratio of (1-20):

1.

5. The composition according to claim 4, characterized in that The dehydrated lutein is dehydrated lutein I, and the dehydrogenated lutein is 3'-dehydrogenated lutein.

6. The composition according to claim 4 or 5, characterized in that The composition further comprises ethyl lutein; in the composition, the mass ratio of dehydrolutein to ethyl lutein is (2-5):1; Preferably, the ethyl lutein is 3'-ethyl lutein.

7. Any of the following uses of the composition according to any one of claims 4 to 6: (1) Application in coloring animal tissues or improving the chromaticity of animal tissues; (2) Application in the preparation of products with animal tissue coloring function.

8. The use according to claim 7, characterized in that: The animal is poultry, preferably chicken, duck, goose, quail or pigeon; And / or, the tissue is skin or a skin derivative; preferably beak, tarsus or underwing skin.

9. Use of the composition according to any one of claims 4 to 6 in the preparation of feed or feed additives.

10. A product, characterized in that The product comprises the composition according to any one of claims 4 to 6.

Images