Skin care composition for improving female climacteric aging and application thereof

Abstract

The invention relates to a skin care composition for improving female climacteric aging and application thereof, and the skin care composition for improving female climacteric aging comprises the following components: conotoxin peptide, bakuchiol, alfalfa extract and radix pseudostellariae extract, the weight ratio of the conotoxin peptide to the bakuchiol to the alfalfa extract to the radix pseudostellariae extract is (0.1-5): (0.1-3): (0.1-5): (0.1-3). By means of inhibiting neurotransmitters, regulating hormones and the like, skin health is enhanced, the problem of skin aging of menopause women is effectively solved, and the skin is tight and smooth.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a skin care composition for improving menopausal aging in women and an application thereof. Background Art

[0002] Menopause is an important stage in a woman's life, usually occurring between the ages of 45 and 55. Due to the decline in estrogen levels in the body, many women may experience a series of skin problems. First, the reduction of collagen and elastin makes the skin lose its elasticity. Studies have shown that within the first 5 years after menopause, type I and type III collagen decrease by up to 30%, resulting in wrinkles and sagging, especially in the corners of the eyes, mouth and forehead. Fine lines and wrinkles will become more obvious, and the firmness of the facial contour will be weakened. Secondly, dryness and dehydration of the skin are very common. The decrease in estrogen levels leads to a decrease in the skin's ability to retain moisture, making the skin rough and lack luster, and may appear peeling or scaly. In addition, the skin of many women becomes more sensitive and more responsive to external stimuli, resulting in discomfort such as redness, itching and tingling, and may even develop skin problems such as eczema. The skin's sensitivity to light exposure may also increase, making the skin more fragile and easily sunburned in the sun. Many women before and after menopause report that they have experienced a "cliff-like" aging, especially the face, which bears the main social responsibilities, which brings great physical and psychological challenges.

[0003] Numerous studies have shown that hormone replacement therapy (HRT) involving estrogen can increase skin collagen levels and thickness, but due to the increased risk of cancers such as breast cancer and uterine cancer, long-term use of HRT is obviously extremely unsafe. As a safe alternative, natural phytoestrogens have similar structures and functions to human estrogen, and can work in the body in a bidirectional regulatory manner, both to supplement the lack of estrogen and to inhibit the problems caused by excess estrogen. Therefore, the topical use of skin care products containing phytoestrogens-like ingredients is an effective measure to improve the aging caused by menopausal estrogen deficiency. It is also safer than oral administration and easier to stick to. In addition to estrogen supplementation, the most important problems of menopausal skin loss, sagging, dryness, and sensitivity also need to be addressed in a targeted manner to comprehensively improve the skin aging problems of menopausal women.

[0004] Currently, most of the products on the market are health foods, and there are no products developed in the field of skin care products to specifically improve the aging problems of menopausal women. Summary of the invention

[0005] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a skin care composition for improving menopausal aging in women and its application. The skin care composition can be applied to skin care products to improve the rapid aging problem of the skin of menopausal women.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] In a first aspect, the present invention provides a skin care composition for improving menopausal aging in women, comprising the following components: conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria baicalensis extract, wherein the weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria baicalensis extract is (0.1-5): (0.1-3): (0.1-5): (0.1-3).

[0008] Preferably, the weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is (0.5-3): (0.1-1): (1-2): (0.5-2).

[0009] More preferably, the weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is (1-2): (0.5-1): (1.5-2): (0.5-1).

[0010] Preferably, the manufacturer of the conotoxin peptide is Shenzhen Weiqi Technology Co., Ltd., and the model is conotoxin peptide.

[0011] Preferably, the manufacturer of the Pseudostellariae Radix extract is Clariant Chemicals (China) Co., Ltd., and the model is CycloRetin TM .

[0012] Preferably, the manufacturer of the bakuchiol is Sytheon, model A.

[0013] Preferably, the manufacturer of the alfalfa extract is Shanghai Jiakai Biological Co., Ltd., model

[0014] The conotoxin peptide in the skin care composition of the present invention can effectively improve the problem of facial sagging by blocking the release of neurotransmitters in facial nerve cells, reduce excessive muscle contraction, and thus smooth the skin surface and improve the firmness of the contour. Bakuchiol activates the estrogen receptors in the skin, increases the moisture content and elasticity of the skin, and slows down the formation of wrinkles. Alfalfa is rich in phytoestrogens, which can regulate the hormone balance in the body, improve the skin's water retention capacity, and keep the skin smooth and firm. Pseudostellariae extract enhances skin immunity, promotes blood circulation, reduces sensitivity and repairs the skin barrier. The above four ingredients work together to effectively improve the skin aging problem of menopausal women by inhibiting the release of neurotransmitters, regulating hormone levels and enhancing skin health, making the skin firmer and smoother.

[0015] In a second aspect, the present invention provides use of the skin care composition for improving menopausal aging in women in the first aspect in the preparation of skin care products.

[0016] Preferably, the skin care product is at least one of lotion, emulsion, cream, mask, essence or spray, and the amount of the skin care composition added is 0.5-5% of the total weight of the skin care product.

[0017] In a third aspect, the present invention provides a facial cream comprising the following raw materials in weight percentage: 0.5%-5% of the skin care composition for improving menopausal aging in women described in the first aspect, 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 1-10% of an emulsifier, 0.5-3% of a preservative and 0.01-0.3% of a pH regulator, and the remainder being deionized water.

[0018] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol.

[0019] Preferably, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding pullulan polysaccharide, and ceramide.

[0020] Preferably, the pH adjuster comprises at least one of arginine, tromethamine, and EDTA-disodium.

[0021] Preferably, the emulsifier includes at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetra(ethylhexanoate), polydimethylsiloxane, stearyl alcohol, sucrose stearate, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate;

[0022] Preferably, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone.

[0023] In a fourth aspect, the present invention provides a method for preparing the facial cream in the third aspect, comprising the following steps:

[0024] S1. After the humectant, thickener and part of deionized water are mixed evenly, the mixture is homogenized at 80°C to form a uniform aqueous phase, the emulsifier is mixed evenly at 80°C, and the aqueous phase and the emulsifier are mixed evenly at 75-85°C to obtain a mixture;

[0025] S2. Add the preservative to the mixture obtained in step S1 and stir evenly. After the temperature drops to 35-45° C., add the components of the skin care composition and the remaining deionized water and stir evenly. Finally, add a pH adjuster to adjust the pH to obtain the facial cream.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The conotoxin peptide in the skin care composition of the present invention can block the release of neurotransmitters, improve facial relaxation, reduce excessive muscle contraction, and improve contour firmness; bakuchiol activates estrogen receptors, enhances skin moisture and elasticity, and slows down wrinkles; alfalfa regulates hormone balance, improves water retention, and keeps the skin smooth and firm; and Pseudostellariae Radix extract enhances immunity, promotes blood circulation, reduces sensitivity, and repairs the skin barrier. These four enhance skin health by inhibiting neurotransmitters, regulating hormones, and other means, effectively improving the problem of skin aging in menopausal women, and making the skin firm and smooth. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The keratinocyte healing conditions of Example 1, Comparative Example 1 and the blank group;

[0029] Figure 2 The facial improvements of the subjects in Application Example 1 and Comparative Application Example 5 are shown. DETAILED DESCRIPTION

[0030] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0031] The sources of the main raw materials used in the following examples and comparative examples are as follows:

[0032] Conotoxin peptide: The manufacturer is Shenzhen Weiqi Technology Co., Ltd., and the model is conotoxin peptide;

[0033] Pseudostellariae Radix Extract: Manufacturer: Clariant Chemicals (China) Co., Ltd., model: CycloRetin TM ;

[0034] Bakuchiol: The manufacturer is Sytheon, model number is A;.

[0035] Alfalfa extract: The manufacturer is Shanghai Jiakai Biological Co., Ltd., model

[0036] Unless otherwise specified, other materials, reagents, etc. used in the examples and comparative examples can be obtained from commercial sources.

[0037] Example 1

[0038] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 1.5:0.6:1.6:0.8, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0039] Example 2

[0040] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 0.1:0.1:5:3, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0041] Example 3

[0042] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 5:3:0.1:0.1, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0043] Example 4

[0044] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 0.5:0.1:1:0.5, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0045] Example 5

[0046] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 3:1:2:2, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0047] Example 6

[0048] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 1:0.5:1.5:0.5, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0049] Example 7

[0050] A skin care composition for improving menopausal aging in women, comprising conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract in a weight ratio of 2:1:2:1, wherein the total weight proportion of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is 50 parts.

[0051] Comparative Example 1

[0052] The difference between Comparative Example 1 and Example 1 is that the total weight of the skin care composition remains unchanged, conotoxin peptide is not added, and bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract are used in a weight ratio of 0.6:1.6:0.8 to make up for the missing amount.

[0053] Comparative Example 2

[0054] The difference between Comparative Example 2 and Example 1 is that the total weight of the skin care composition remains unchanged, bakuchiol is not added, and conotoxin peptide, alfalfa extract and Pseudostellaria heterophylla extract are used in a weight ratio of 1.5:1.6:0.8 to make up for the missing amount.

[0055] Comparative Example 3

[0056] The difference between Comparative Example 3 and Example 1 is that the total weight of the skin care composition remains unchanged, no alfalfa extract is added, and the missing amount is made up by using conotoxin peptide, bakuchiol and Pseudostellaria heterophylla extract in a weight ratio of 1.5:0.6:0.8.

[0057] Comparative Example 4

[0058] The difference between Comparative Example 4 and Example 1 is that the total weight of the skin care composition remains unchanged, no Pseudostellaria heterophylla extract is added, and conotoxin peptide, bakuchiol and alfalfa extract are used in a weight ratio of 1.5:0.6:1.6 to make up for the missing amount.

[0059] Test Example 1

[0060] The raw material components in the skin care compositions of Examples 1-7 and Comparative Examples 1-4 were mixed according to the formula amount, and DMSO of 1 times the mass of the skin care composition was added and mixed to obtain a skin care composition test sample. The skin care composition test sample was only used for in vitro testing.

[0061] 1. Estrogen receptor 2 (ESR2) expression rate test in cells

[0062] Activation of facial estrogen receptors plays an important role in improving menopausal aging. Estrogen helps improve skin elasticity and firmness, slow down wrinkles and dryness, and improve skin tone and texture by promoting collagen production and enhancing skin moisture. In addition, estrogen regulates oil secretion, reduces acne and oiliness, enhances antioxidant capacity, and resists free radical damage. Therefore, proper promotion of estrogen receptors on the skin can play a role in improving the aging state of menopausal skin.

[0063] The skin care composition test samples of Example 1 and the skin care composition test samples of Comparative Examples 1-4 were subjected to experiments according to the following steps. The results are shown in Table 1.

[0064] Human keratinocytes HaCaT were revived (Guangzhou Geneo Biotechnology Co., Ltd.), and the test conditions were: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%; cells were cultured and treated according to the grouping, and then tested. The specific test was estrogen receptor 2 (ESR2) expression, and the test method was as follows:

[0065] (1) The cell suspension was inoculated into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of DMEM high-glucose culture medium (Gibco) containing 10% fetal bovine serum was added to each well and cultured for 24 h.

[0066] (2) Sampling: Add 100 μL of culture solution to the blank control group, and add 100 μL of culture solution containing 0.05% of the skin care composition test sample to the sample group, and continue culturing for 24 hours;

[0067] (3) Detection of Estrogen Receptor 2 (ESR2) Gene Expression: Real-time fluorescence quantitative PCR was used to detect the expression of estrogen receptor 2 (ESR2) gene in HaCaT cells of each group. RNA reverse transcription kit (Applied Biosystems) was used for RT reaction, and ESR2 was detected using specific primers and probe Hs01584024_m1 provided by Applied Biosystems. -ΔΔCt Calculate the relative gene expression at the mRNA level.

[0068] The promoting effect of the sample on estrogen is expressed by the increase rate of ESR2 receptors, and the calculation formula is as follows:

[0069] Estrogen receptor increase rate (%) = (ESR2 expression 样品组 / ESR2 expression 空白对照组 -1)*100%

[0070] Table 1 ESR2 increase rate data of each group of skin care compositions

[0071] Group / Performance ESR2 increase rate / % Example 1 23.91 Comparative Example 1 0.73 Comparative Example 2 2.41 Comparative Example 3 0.10 Comparative Example 4 1.32

[0072] 2. Cell scratch healing test

[0073] The cell scratch test can effectively evaluate the skin barrier repair ability. By observing the migration and regeneration of cells after scratching, it can directly reflect the ability of cells to repair damaged barriers. In the experiment, by comparing the cell healing speed, the effects of different treatments on skin repair can be evaluated.

[0074] The skin care composition test samples of Examples 1-7 and the skin care composition test samples of Comparative Examples 1-4 were subjected to experiments according to the following steps. The results are shown in Table 2.

[0075] Human keratinocytes HaCaT were revived (Guangzhou Geneo Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1°C, humidity 90±5%, carbon dioxide 5±1%. Cells were cultured and treated according to the grouping, and then tested. The specific test was to use a cell healing 2-hole insert. The test method is as follows:

[0076] (1) The cell suspension was inoculated into a 2-well cell healing plate at a density of 6×10 5 100 μL of DMEM high-glucose culture medium (Gibco) containing 10% fetal bovine serum was added to each well and cultured for 24 h;

[0077] (2) Administration: 100 μL of culture medium was added to the blank control group, and 100 μL of culture medium containing 0.05% of the skin care composition test sample was added to the sample group, the insert was removed, and the culture was continued for 24 hours;

[0078] (3) Photography: The cell scratches of each group were photographed under a microscope at 0 h and 24 h, and the scratch area was measured.

[0079] The barrier repair ability of the sample on the skin is expressed by the cell scratch healing rate, and the calculation method is as follows:

[0080] Cell healing rate (%) = (cell scratch area 0h -Cell scratch area 24h ) / cell scratch area oh

[0081] Table 2 Cell scratch healing rate data of each group of skin care compositions

[0082] Group / Performance Cell scratch healing rate / % Example 1 56.23 Example 2 23.94 Example 3 27.41 Example 4 35.03 Example 5 37.25 Example 6 42.61 Example 7 47.32 Comparative Example 1 12.31 Comparative Example 2 10.42 Comparative Example 3 9.42 Comparative Example 4 9.03 Blank control group 7.32

[0083] 3. Collagen and elastin expression test

[0084] 75% of the skin's dermis is composed of collagen. Fibrous collagen and elastin form a network structure that provides tension to the skin and gives the tissue elasticity. The two are mixed together to form the basic substance of the skin, maintaining the structural integrity of the skin's dermis and making the skin elastic and firm. Type I collagen is the most abundant protein in the skin. The distribution of type I collagen in zebrafish is the same as that in humans and shows a high degree of conservation with humans. The elastin gene is also highly conserved. By testing the expression of the zebrafish type I collagen gene (col1a1a) and the corresponding elastin gene (Eln1), it can be judged whether the sample has the effect of promoting the regeneration of collagen and elastin, reshaping the dermis structure, and anti-wrinkle and firming the skin.

[0085] Therefore, this effect example evaluates the efficacy of the composition prepared in Examples 1-7 and Comparative Examples 1-4 by performing zebrafish collagen and elastin gene detection, and the experiment is carried out according to the conditions in Table 3. The results are shown in Table 3; specifically, the following steps are included:

[0086] (1) Sample treatment: The test samples of the composition prepared in Examples 1-7 and Comparative Examples 1-4 were mixed with water at a mass ratio of 0.5% by high speed homogenization, and then ultrasonically treated for 10 minutes. Then, fish embryo culture medium was added to prepare the required concentration, and after shaking for 30 seconds, centrifuged at 6500r / min for 10 minutes, and the supernatant was taken for testing. The concentration of the test solution of the test substance must ensure that the mortality rate of zebrafish embryos is less than 10% (embryos without heartbeat characteristics are defined as dead), and 3 concentration groups were set during the test.

[0087] (2) Test steps:

[0088] 1) Select healthy zebrafish embryos that are 6 days old after fertilization. The test requires setting up a blank control group (fish embryo culture medium) and a sample group (test sample). Each well contains 6 zebrafish and 2.5mL fish embryo culture medium. Place in a 28±1℃ incubator and culture until 24±1h after fertilization. Collect the 6 zebrafish in each well into a 1.5mL tube, remove the solution, add 0.5mL RNAlater solution, and store frozen.

[0089] 2) Remove RNAlater solution and rinse with PBS solution 3 times. Place in ice bath, remove PBS solution, and add 500μL TRIzol reagent. Homogenize embryos with a pellet pestle and place in ice bath for 10 minutes. Add 100μL chloroform, vortex for 1 minute, and place in ice bath for 5 minutes. Centrifuge samples for 20 minutes, transfer the upper layer solution to a 1.5mL centrifuge tube, add 250μL isopropanol, vortex for 1 minute, place in ice bath for 10 minutes, and centrifuge samples for 20 minutes. Remove all supernatant, add 500μL ethanol, vortex for 1 minute, centrifuge samples for 5 minutes, and place in ice bath for 10 minutes. Repeat this step 3 times. Remove ethanol, centrifuge samples for 5 minutes, and air dry samples in a fume hood with the lid open for 10 minutes. Add 10μL DNase / RNase-free ultrapure distilled water and heat at 55℃ for 15 minutes. Dilute RNA samples to 1000ng / 7μL with DNase / RNase-free ultrapure distilled water. cDNA was synthesized from RNA using the PrimerScript RT Kit with gDNAEraser (Takara; Cat no.RR047A) and stored frozen. Primer mix and real-time PCR master mix were prepared for each primer. The cDNA samples were diluted 10-fold with DNase / RNase-free ultrapure distilled water. 9 μL of PCR master mix and 1 μL of diluted cDNA sample were added to each well of a 96-well plate for PCR. The 96-well plate for PCR was sealed with optical film, centrifuged for 5 minutes, and real-time PCR amplification was performed. The gene sequences were as follows: β-actin: 5'-GCTGAC AGGATGCAGAAGGA-3' and 5'-TAGAAGCATTTGCGGTGGAC-3', type I collagen gene (col1a1a) 5'-TAGCCCCTATGGACGTTGGT-3' and 5'-CGCAGGTCTAAG CAAGTGGA-3' and elastin gene (Eln1) 5'-AAAACCAGGTTACGGCTCTGT-3' and 5'-TCCTCCTGGATAAGCTCCGTATC-3'. β-actin was used as the internal reference gene to calculate the relative expression of the target gene.

[0090] The promotion of collagen and elastin in the skin by the composition is expressed by the gene promotion rate, which is calculated as follows:

[0091] Promotion rate (%) = (test group gene expression - blank gene expression) / blank group gene expression × 100%

[0092] Table 3 Data on the promotion rate of collagen and elastin in each group of skin care compositions

[0093] Group / Performance Collagen promotion rate / % Elastin promotion rate / % Example 1 73.23 128.04 Example 2 37.95 72.41 Example 3 45.29 89.47 Example 4 47.93 94.76 Example 5 52.94 97.32 Example 6 61.83 101.84 Example 7 63.05 102.94 Comparative Example 1 24.95 32.30 Comparative Example 2 21.36 31.94 Comparative Example 3 17.43 27.84 Comparative Example 4 15.21 23.94

[0094] From the data of Examples 1-7 and Comparative Examples 1-4 in Table 1-3, it can be seen that the four components of the skin care composition have a significant effect on the composition's promotion of estrogen, barrier repair, collagen supplementation and relaxation improvement. When conotoxin peptides, bakuchiol, alfalfa extract and Pseudostellariae Radix extract are used in combination, the anti-aging effect of the composition is better. When one of the four components is missing, the anti-aging performance of the composition decreases. This may be because there is a synergistic effect between conotoxin peptides, bakuchiol, alfalfa extract and Pseudostellariae Radix extract, which can appropriately upregulate the expression of estrogen receptor 2 (ESR2) in skin keratinocytes; promote the mobility of cells in skin keratinocytes; and promote the healing of skin injuries. In addition, the composition can also promote the expression of collagen I and III and elastin in dermal fibroblasts. Therefore, while supplementing estrogen to the composition, collagen and elastin can also be supplemented in a targeted manner, which can effectively improve the anti-aging effect of the composition and improve the aging problems of menopausal women.

[0095] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria baicalensis extract is (0.5-3):(0.1-1):(1-2):(0.5-2), the estrogen supplementation and anti-aging effects of the skin care composition can reach a better level, and more preferably the weight ratio of conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria baicalensis extract is (1-2):(0.5-1):(1.5-2):(0.5-1).

[0096] Application Example 1 and Comparative Application Examples 1-4

[0097] The skin care compositions of Example 1 and Comparative Examples 1-4 were added to the facial cream at a concentration of 5 wt %, respectively, to obtain the facial creams of Application Example 1 and Comparative Application Examples 1-4. The formulas are shown in Table 4.

[0098] The preparation method of the facial cream of Application Example 1 and Comparative Application Examples 1-4 comprises the following steps:

[0099] The preparation method of the facial cream comprises the following steps:

[0100] S1. After the humectant, thickener and part of deionized water are mixed evenly, the mixture is homogenized at 80° C. to form a uniform aqueous phase, the emulsifier is mixed evenly at 80° C., and the aqueous phase and the emulsifier are mixed evenly at 80° C. to obtain a mixture;

[0101] S2. Add the preservative to the mixture obtained in step S1 and stir evenly. After the temperature drops to 40° C., add the components of the skin care composition and the remaining deionized water and stir evenly. Finally, add a pH adjuster to adjust the pH to obtain the facial cream.

[0102] Table 4 Cream formula of application example 1 and comparative application examples 1-4

[0103]

[0104] Comparative Application Example 5

[0105] The facial cream of comparative application example 5 does not contain the skin care composition, but uses an equal amount of deionized water instead of the skin care composition. The preparation method is the same as that of application example 1.

[0106] Test Example 2

[0107] This test example verifies the improvement effect of the creams of Application Example 1 and Comparative Application Examples 1-5 on facial aging and barrier damage in menopausal female subjects. The experimental data are shown in Table 5.

[0108] According to the "Technical Specifications for Safety of Cosmetics" (2015), 48 female subjects aged 45-65 years old and in menopause were selected for the test, and the screening method was a self-assessment questionnaire. The subjects were randomly divided into 6 groups, with 8 people in each group. The volunteers used samples on their entire face once in the morning and evening every day (the samples were Application Example 1 and Comparative Application Examples 1-5), and visited to collect data on the 0th and 28th days of use. After the visit, the volunteers washed their faces with facial cleanser and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. Before and after use, the subjects used VISIA-CR to take pictures of the wrinkles under the eyes to analyze the wrinkle area and calculate the improvement rate of the wrinkle area under the eyes. Before and after use, the subjects used the skin water loss test probe TewaMeter to measure the transepidermal water loss value TEWL of the cheekbones and calculate the improvement rate of facial TEWL. Before and after use, the subjects used the skin stratum corneum moisture measurement probe CM825 measures skin moisture content and calculates the improvement rate of facial moisture content. The subjects used the skin elasticity test probe before and after use MPA580 measures skin firmness F4 and calculates the improvement rate of firmness F4. The calculation method of the improvement rate of each value is as follows: Improvement rate = |(T0 测量值 -T28 测量值 )| / T0 测量值 *100%. The data are shown in Table 5.

[0109] In this test example, the improvement rate of firmness F4, the improvement rate of under-eye wrinkle area, the improvement rate of facial moisture content and the improvement rate of facial TEWL are used to indicate the improvement of the facial cream prepared by the composition on sagging, wrinkles, dryness and barrier damage of the subject's face.

[0110] Table 5 Human test data of each group of facial cream samples

[0111]

[0112]

[0113] Experimental conclusion: From Table 5, Figure 1-2 It can be seen that the face cream prepared using the skin care composition for improving menopausal aging in women of Example 1 has a significantly better anti-aging effect than the face cream prepared using the skin care composition of Comparative Examples 1-4 having only three functional ingredients, which is consistent with the result of Test Example 1. This also shows that the four ingredients of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellariae Radix extract of the present invention have a synergistic effect, and the face cream added with this composition can solve the most important problems of collagen and elastin loss, relaxation, dryness and sensitivity and fragility of menopausal skin, and achieve the effects of anti-aging, anti-wrinkle, moisturizing and barrier repair, thereby comprehensively improving the skin aging problems of menopausal women.

[0114] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A skin care composition for improving female menopausal aging, characterized in that: The invention comprises the following components: conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellariae Radix extract, wherein the weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellariae Radix extract is (0.1-5): (0.1-3): (0.1-5): (0.1-3).

2. The skin care composition for improving female menopausal aging according to claim 1, characterized in that: The weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is (0.5-3): (0.1-1): (1-2): (0.5-2).

3. The skin care composition for improving menopausal aging in women according to claim 1, characterized in that: The weight ratio of the conotoxin peptide, bakuchiol, alfalfa extract and Pseudostellaria heterophylla extract is (1-2): (0.5-1): (1.5-2): (0.5-1).

4. The skin care composition for improving menopausal aging in women according to claim 1, characterized in that: The manufacturer of the conotoxin peptide is Shenzhen Weiqi Technology Co., Ltd., and the model is conotoxin peptide; and / or, the manufacturer of the Pseudostellaria heterophylla extract is Clariant Chemicals (China) Co., Ltd., and the model is CycloRetin TM .

5. The skin care composition for improving menopausal aging in women according to claim 1, characterized in that: The manufacturer of the bakuchiol is Sytheon, and the model is A; and / or, the manufacturer of the alfalfa extract is Shanghai Jiakai Biological Co., Ltd., model 6. Use of the skin care composition for improving female menopausal aging according to any one of claims 1 to 5 in the preparation of skin care products.

7. Use of a skin care composition for improving menopausal aging in women according to claim 6 in the preparation of skin care products, characterized in that: The skin care product is at least one of lotion, emulsion, cream, mask, essence or spray, and the added amount of the skin care composition is 0.5-5% of the total weight of the skin care product.

8. A facial cream, characterized in that: The invention comprises the following raw materials in weight percentage: 0.5%-5% of the skin care composition for improving menopausal aging of women according to any one of claims 1 to 5, 0.05-0.3% of a thickener, 0.5-1% of a moisturizer, 1-10% of an emulsifier, 0.5-3% of a preservative and 0.01-0.3% of a pH regulator, and the balance is deionized water.

9. The facial cream according to claim 8, characterized in that The raw material is selected from at least one of (a) to (e): (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butylene glycol, glycerol, budding pullulan polysaccharide, and ceramide; (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium; (d) the emulsifier comprises at least one of caprylic / capric triglyceride, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythritol tetra(ethylhexanoate), polydimethylsiloxane, stearyl alcohol, sucrose stearate, hydroxystearic acid, polymethylsilsesquioxane, pentaerythritol distearate, and sucrose stearate; (e) The preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone.

10. The method for preparing the facial cream according to claim 9, characterized in that: The following steps are involved: S1. After the humectant, thickener and part of deionized water are mixed evenly, the mixture is homogenized at 80°C to form a uniform aqueous phase, the emulsifier is mixed evenly at 80°C, and the aqueous phase and the emulsifier are mixed evenly at 75-85°C to obtain a mixture; S2. Add the preservative to the mixture obtained in step S1 and stir evenly. After the temperature drops to 35-45° C., add the components of the skin care composition and the remaining deionized water and stir evenly. Finally, add a pH adjuster to adjust the pH to obtain the facial cream.

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