Plant composition for regulating scalp micro-ecological balance and application thereof

Abstract

The invention relates to the field of plant extracts, and discloses a plant composition for regulating scalp micro-ecological balance and application thereof. The plant composition disclosed by the invention takes extracts of leontopodium alpinum and birch bark as active ingredients, has the characteristics of effectively inhibiting staphylococcus aureus and restrictive malassezia and not influencing normal proliferation of staphylococcus epidermidis, so that the plant composition can be used for regulating scalp micro-ecological balance, regulating and controlling scalp microbial flora in a targeted manner and improving scalp microecological balance. Therefore, the scalp grease secretion is reduced, and the scalp problems such as dandruff, scalp itching, scalp papule, allergy and redness, exuberant grease secretion and the like are relieved.

Description

Technical Field

[0001] The present invention relates to the field of plant extracts, and in particular to a plant composition for regulating scalp microecological balance and application thereof. Background Art

[0002] The scalp microecology is a complex ecosystem composed of microorganisms, human scalp tissue, hair follicles, sebaceous glands and other skin appendages, as well as various secretions. Studies have shown that there are about 1 million microorganisms per square centimeter of scalp, which together form the scalp microecology and form a barrier on the scalp surface. A healthy scalp ecological environment is maintained by three major balances: oil balance, bacterial balance and metabolic balance.

[0003] A large number of research results show that fungi and bacteria are symbiotic residents on most healthy scalps, mainly including Staphylococcus, Propionibacterium and Malassezia. These microorganisms generally do not cause scalp problems, but when the scalp microecological balance is broken, such as excessive sebum secretion and excessive microbial proliferation, it can lead to the occurrence of scalp problems such as dandruff, oily scalp, itchy scalp, hair loss, seborrheic dermatitis, etc.

[0004] With changes in the environment, people's lifestyles, and their own stress, most people have problems with both scalp and hair. Regulating the scalp microecology aims to improve the balance of scalp flora, inhibit harmful bacteria, and promote the growth of beneficial bacteria, thereby repairing or enhancing the scalp barrier function. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a plant composition for regulating the microecological balance of the scalp and its application. The plant composition of the present invention uses extracts of alpine edelweiss and birch bark as active ingredients, has the characteristics of effectively inhibiting Staphylococcus aureus and restrictive Malassezia, and does not affect the normal proliferation of Staphylococcus epidermidis. Therefore, it can be used to regulate the microecological balance of the scalp, target and regulate the scalp microbial flora, thereby reducing the oil secretion of the scalp, and relieving dandruff, scalp itching, scalp papules, sensitive redness, and vigorous oil secretion and other scalp problems.

[0006] The specific technical scheme of the present invention is: In a first aspect, the present invention provides a plant composition for regulating the microecological balance of the scalp, comprising extracts of alpine edelweiss and birch bark; the extraction part of the alpine edelweiss is the alpine edelweiss flower and / or the alpine edelweiss leaf.

[0007] The excessive proliferation of Staphylococcus aureus and Malassezia restricta will disrupt the microecological balance of the scalp and promote oil secretion, thereby causing scalp problems such as dandruff, oily scalp, and itchy scalp. Therefore, Staphylococcus aureus and Malassezia restricta are harmful microorganisms for the scalp. At the same time, according to the previous research of the present invention, Staphylococcus epidermidis can inhibit the growth and local colonization of harmful microorganisms such as Staphylococcus aureus, so Staphylococcus epidermidis is a beneficial bacteria.

[0008] Alpine edelweiss is a perennial herbaceous plant of the genus Edelweiss in the Asteraceae family, native to the European mountains. It is known to have anti-inflammatory and anti-inflammatory effects, and is often used in traditional Chinese medicine and cosmetics. Birch is a tree of the genus Betula in the Betulaceae family. Its bark contains chemical components such as tannins, betulin, betulin and oleanolic acid. It is known to have the effects of clearing heat and dampness, removing phlegm and relieving cough, detoxifying and reducing swelling, and is also often used in traditional Chinese medicine.

[0009] The present invention accidentally discovered through experiments that extracts of alpine edelweiss and birch bark as active ingredients can effectively regulate the balance of scalp microecology and target the regulation of scalp microbial flora. Specifically, while effectively inhibiting Staphylococcus aureus and Malassezia restricta, it does not affect the normal proliferation of Staphylococcus epidermidis, thereby achieving the effect of alleviating dandruff, scalp itching, and excessive oil secretion and other scalp problems.

[0010] Specifically, Malassezia restricta is a skin symbiotic fungus that lacks the fatty acid synthase gene. The lipase it contains can degrade triglycerides in sebum. Triglycerides are hydrolyzed into unsaturated fatty acids such as oleic acid and arachidonic acid, which destroy the barrier function of the epidermal stratum corneum and cause various scalp problems. The plant composition of the present invention inhibits the proliferation of Malassezia restricta through the synergistic effects of the following aspects: (1) Malassezia restricta lacks the relevant genes of fatty acid synthase and needs to rely on exogenous lipids. The plant composition of the present invention has an oil control effect and can reduce the secretion of scalp oil, thereby reducing the proliferation rate of Malassezia restricta on the scalp. (2) The plant composition of the present invention can inhibit the lipase activity of Malassezia restricta, reduce its ability to utilize lipids, reduce the free fatty acids (such as oleic acid and palmitic acid) decomposed by triglycerides in sebum, thereby blocking the formation of various scalp problems mediated by oleic acid and palmitic acid. (3) The plant composition of the present invention can effectively destroy the integrity of the cell wall, change the permeability of the cell membrane, and inhibit the growth of Malassezia restricta in multiple ways.

[0011] The present invention further discovered through research that the above two active ingredients in the plant composition of the present invention have a synergistic effect. If one of the active ingredients is used alone or one of the active ingredients is replaced by other plant extracts, the above technical effect cannot be achieved.

[0012] Preferably, the mass ratio of the alpine edelweiss to the birch bark is 1:2 to 5:1; further preferably, the mass ratio of the alpine edelweiss to the birch bark is 1:1 to 4:1.

[0013] In order to further enhance the synergistic effect of the alpine edelweiss extract and the birch bark extract, the present invention optimizes the ratio of the two, so as to better inhibit the proliferation of Staphylococcus aureus and Malassezia restricta through multiple pathways and multiple targets.

[0014] Preferably, the extract is an alcohol extract.

[0015] In a second aspect, the present invention provides a method for preparing the above-mentioned plant composition, comprising the following steps: crushing alpine edelweiss and birch bark respectively, sieving and mixing; using alcohol aqueous solution as extraction solvent, ultrasonic extraction, filtering, concentrating the extract and drying.

[0016] Preferably, the sieving is through a 10-40 mesh sieve.

[0017] Preferably, the alcohol is selected from one or more alcohols such as ethanol, propylene glycol and butylene glycol.

[0018] Preferably, the mass ratio of the alcohol aqueous solution to the raw material is 5-15:1; and the volume concentration of the alcohol aqueous solution is 40-60%.

[0019] Preferably, the conditions for ultrasonic extraction are: ultrasonic power of 100-400 W, ultrasonic temperature of 45-60° C., ultrasonic time of 20-50 min, and ultrasonic times of 1-5 times.

[0020] Preferably, the concentration is concentration to an extract form.

[0021] Preferably, the drying is freeze-drying.

[0022] In a third aspect, the present invention provides another method for preparing the above-mentioned plant composition, comprising the following steps: respectively crushing alpine edelweiss and birch bark, sieving, mixing, adding to an alcohol aqueous solution, performing liquid phase pulse discharge extraction, filtering, concentrating the extract, and drying.

[0023] The present invention finds that, compared with a conventional ultrasonic extraction process, the extraction rate of active ingredients in the plant extract finally obtained by the liquid phase pulse discharge extraction process can be significantly improved.

[0024] Preferably, the sieving is through a 10-40 mesh sieve.

[0025] Preferably, the alcohol is selected from one or more alcohols such as ethanol, propylene glycol and butylene glycol. Preferably, the mass ratio of the alcohol aqueous solution to the raw material is 5-10:1; and the volume concentration of the alcohol aqueous solution is 20-40%.

[0026] Preferably, the liquid phase pulse discharge extraction adopts a multi-stage differentiation procedure: (a) Discharge voltage 1-2KV, temperature 15-20℃, time 0.5-1.5min; (b) Discharge voltage 3-5KV, temperature 25-30℃, time 2-4min; (c) Discharge voltage 5-7KV, temperature 30-35℃, time 4-5min; (d) Discharge voltage 7-8KV, temperature 35-45℃, time 6-8min.

[0027] In order to further improve the extraction rate, the present invention selects the liquid phase pulse discharge extraction process, and according to the characteristics of different stages in the extraction process of the process, the above-mentioned multi-stage differentiation parameters are designed in a targeted manner. The principle is: in the liquid phase pulse discharge extraction process, the discharge voltage determines the intensity of secondary phenomena such as shock waves, cavitation and chemical reaction substances in the extraction process, thereby affecting the destruction efficiency of plant tissues and the mass transfer rate of active ingredients. According to the inventor's research, liquid phase pulse discharge extraction is divided into the following four stages: pre-breakdown stage (streamer) → breakdown stage (arc) → disintegration of cell structure → enhanced mass transfer process.

[0028] In the first stage, the discharge voltage and extraction time are set within the above ranges because this stage is the formation and propagation of streamers; it injects energy directly into the aqueous solution through the plasma channel formed by the high current / high voltage discharge between the two immersed electrodes, and the ionized gas channel generated by the streamer constitutes a conductive path. In the second stage, the discharge voltage is further increased and the extraction time is extended. When the streamer reaches the grounded surface electrode, an arc is generated in the streamer channel (breakdown phase). This stage is the formation and propagation of the arc; in the third stage, with the increase of the discharge voltage and the extension of the extraction time, the product is broken, the cells are broken, and the cell structure is disintegrated; in the fourth stage, the mass transfer process is enhanced. Energy input is one of the most important parameters to be considered in the optimization of discharge extraction. Discharge pretreatment can obtain a higher solute yield in a shorter diffusion time.

[0029] In a fourth aspect, the present invention provides the use of the above-mentioned plant composition in the targeted inhibition of Staphylococcus aureus and Malassezia restricta.

[0030] In a fifth aspect, the present invention provides the use of the above-mentioned plant composition in cosmetics: the cosmetics are products that have the ability to target and inhibit Staphylococcus aureus and Malassezia restricta, and do not affect the normal proliferation of Staphylococcus epidermidis.

[0031] Compared with the prior art, the beneficial effects of the present invention are: (1) The plant composition of the present invention uses extracts of alpine edelweiss and birch bark as active ingredients, has the characteristics of effectively inhibiting Staphylococcus aureus and restrictive Malassezia, and does not affect the normal proliferation of Staphylococcus epidermidis. Therefore, it can be used to regulate the balance of scalp microecology and target the regulation of scalp microbial flora, thereby reducing scalp oil secretion and alleviating scalp problems such as dandruff, scalp itching, scalp papules, sensitive redness, and excessive oil secretion.

[0032] (2) The extracts of alpine edelweiss and birch bark in the plant composition of the present invention have a synergistic effect. If one of the active ingredients is used alone or one of the active ingredients is replaced by other plant extracts, the above technical effect cannot be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 Graph showing the leakage of AKP in restrictive Malassezia cells in each case of the present invention.

[0034] Figure 2 Graph showing the leakage of nucleic acid from restricted Malassezia cells in each case of the present invention. DETAILED DESCRIPTION

[0035] The present invention will be further described below in conjunction with the embodiments.

[0036] Overall embodiment Firstly, the present invention provides a plant composition for regulating the microecological balance of the scalp, comprising extracts of alpine edelweiss and birch bark; the extraction part of the alpine edelweiss is alpine edelweiss flower and / or alpine edelweiss leaf.

[0037] In some preferred implementation cases, the mass ratio of the alpine edelweiss to the birch bark is 1:2 to 5:1; further preferably, the mass ratio of the alpine edelweiss to the birch bark is 1:1 to 4:1.

[0038] Preferably, the extract is an alcohol extract.

[0039] Secondly, the present invention provides a method for preparing the above-mentioned plant composition, comprising the following steps: crushing alpine edelweiss and birch bark respectively, sieving and mixing; using alcohol aqueous solution as extraction solvent, ultrasonic extraction, filtering, concentrating the extract and drying.

[0040] Preferably, the sieving is through a 10-40 mesh sieve.

[0041] Preferably, the alcohol is selected from one or more alcohols such as ethanol, propylene glycol and butylene glycol.

[0042] Preferably, the mass ratio of the alcohol aqueous solution to the raw material is 5-15:1; and the volume concentration of the alcohol aqueous solution is 40-60%.

[0043] Preferably, the conditions for ultrasonic extraction are: ultrasonic power of 100-400 W, ultrasonic temperature of 45-60° C., ultrasonic time of 20-50 min, and ultrasonic times of 1-5 times.

[0044] Preferably, the concentration is concentration to an extract form.

[0045] Preferably, the drying is freeze-drying.

[0046] In addition, the present invention provides another method for preparing the above-mentioned plant composition, comprising the following steps: respectively crushing alpine edelweiss and birch bark, sieving, mixing, adding to an alcohol aqueous solution, performing liquid phase pulse discharge extraction, filtering, concentrating the extract, and drying.

[0047] Preferably, the sieving is through a 10-40 mesh sieve.

[0048] Preferably, the alcohol is selected from one or more alcohols such as ethanol, propylene glycol and butylene glycol.

[0049] Preferably, the mass ratio of the alcohol aqueous solution to the raw material is 5-10:1; and the volume concentration of the alcohol aqueous solution is 20-40%.

[0050] Preferably, the liquid phase pulse discharge extraction adopts a multi-stage differentiation procedure: (a) Discharge voltage 1-2KV, temperature 15-20℃, time 0.5-1.5min; (b) Discharge voltage 3-5KV, temperature 25-30℃, time 2-4min; (c) Discharge voltage 5-7KV, temperature 30-35℃, time 4-5min; (d) Discharge voltage 7-8KV, temperature 35-45℃, time 6-8min.

[0051] Furthermore, the present invention provides the use of the above-mentioned plant composition in targeted inhibition of Staphylococcus aureus and Malassezia restricta.

[0052] Finally, the present invention provides the use of the plant composition in cosmetics: the cosmetics are products that have the ability to target and inhibit Staphylococcus aureus and Malassezia restricta, and do not affect the normal proliferation of Staphylococcus epidermidis.

[0053] Specific Examples and Comparative Examples Example 1 The dried plant materials of alpine edelweiss flowers and birch bark are crushed respectively, passed through a 20-mesh sieve, and the powders are mixed in a mass ratio of 4:1; a 40% (volume ratio) ethanol aqueous solution is added to the above mixture and soaked for 4 hours (10 times the mass ratio), and ultrasonic extraction is performed with an ultrasonic power of 100 W, an ultrasonic temperature of 50°C, an ultrasonic time of 20 min, and 5 ultrasonic times; the extracts are filtered and combined, concentrated under reduced pressure to an extract state, and then freeze-dried to obtain a plant composition.

[0054] Example 2 The dried plant materials of alpine edelweiss flowers and birch bark were crushed respectively, passed through a 40-mesh sieve, and the powders were mixed in a mass ratio of 3:1; a 60% (volume ratio) ethanol aqueous solution was added to the above mixture and soaked for 3 hours (5 times the mass ratio), and ultrasonic extraction was performed with an ultrasonic power of 200 W, an ultrasonic temperature of 45°C, an ultrasonic time of 40 min, and 3 ultrasonic times; the extracts were filtered and combined, concentrated under reduced pressure to an extract state, and then freeze-dried to obtain a plant composition.

[0055] Example 3 The dried plant materials of alpine edelweiss flowers and birch bark are crushed respectively, passed through a 10-mesh sieve, and the powders are mixed in a mass ratio of 2:1; a 50% (volume ratio) ethanol aqueous solution is added to the above mixture and soaked for 6 hours (8 times the mass ratio), ultrasonic extraction is performed, the ultrasonic power is 400 W, the ultrasonic temperature is 55°C, the ultrasonic time is 35 min, the ultrasonic number is 4 times, the extracts are filtered and combined, and the extracts are concentrated under reduced pressure to an extract state and then freeze-dried to obtain a plant composition.

[0056] Example 4 The dried plant materials of alpine edelweiss leaves and birch bark were crushed respectively, passed through a 30-mesh sieve, and the powders were mixed in a mass ratio of 1:1; 55% (volume ratio) ethanol aqueous solution was added to the above mixture and soaked for 5 hours (15 times the mass ratio), ultrasonic extraction was performed, the ultrasonic power was 300 W, the ultrasonic temperature was 60°C, the ultrasonic time was 50 min, the ultrasonic number was 2 times, the extracts were filtered and combined, the reduced pressure was concentrated to an extract state, and then freeze-dried to obtain a plant composition.

[0057] Example 5 The difference from Example 3 is that the mass ratio of the plant raw materials, alpine edelweiss flowers and birch bark, is 5:1, and the rest is the same as Example 3.

[0058] Example 6 The difference from Example 3 is that the mass ratio of the plant raw materials, alpine edelweiss flowers and birch bark, is 1:2, and the rest is the same as Example 3.

[0059] Comparative Example 1 The difference from Example 3 is that the plant raw material is only one raw material, namely, alpine edelweiss flower, and the rest is the same as Example 3.

[0060] Comparative Example 2 The difference from Example 3 is that the plant raw material is only birch bark, and the rest is the same as Example 3.

[0061] Comparative Example 3 The difference from Example 3 is that the plant raw materials are alpine edelweiss flowers and honeysuckle in a mass ratio of 2:1, and the rest are the same as Example 3.

[0062] Comparative Example 4 The difference from Example 3 is that the plant raw materials are mugwort and birch bark in a mass ratio of 2:1, and the rest are the same as Example 3.

[0063] (1) Targeted antibacterial test Staphylococcus aureus, Malassezia restricta and Staphylococcus epidermidis were inoculated in their respective culture media, and Staphylococcus aureus and Staphylococcus epidermidis were cultured in a 37°C constant temperature incubator for 24 hours; Malassezia restricta was placed in a 30°C incubator for 4 days. The minimum inhibitory concentration (MIC) of each test substance was determined by the two-fold dilution method, and each group was paralleled three times. The results of the antibacterial activity test are shown in Table 1.

[0064] Table 1: Antibacterial activity test results Note: - indicates no bacterial growth, + indicates bacterial growth The preliminary study of the present invention shows that Staphylococcus epidermidis can inhibit the growth and local colonization of harmful microorganisms such as Staphylococcus aureus. Therefore, Staphylococcus epidermidis is a beneficial bacterium for dandruff removal.

[0065] It can be seen from the antibacterial test in Table 1 that Examples 1-6 of the present invention can significantly inhibit Staphylococcus aureus and Malassezia restricta, and at the same time have no inhibitory effect on Staphylococcus epidermidis, indicating that Examples 1-6 can target and inhibit the harmful bacteria Staphylococcus aureus and Malassezia restricta that cause scalp problems, without affecting the growth of the beneficial bacteria Staphylococcus epidermidis, and comprehensively regulate the scalp microecological flora to achieve a stable state (among which Examples 1-4 are better than Examples 5-6). The antibacterial effect of Comparative Examples 1-2 using any one component is significantly lower than that of Example 3 (specifically, the MIC value of Comparative Examples 1-2 is higher).

[0066] At the same time, the two raw materials in the plant composition of the present invention are replaced by currently recognized raw materials with similar effects (in Comparative Example 3, birch bark is replaced by an equal amount of honeysuckle; in Comparative Example 4, alpine edelweiss is replaced by an equal amount of wormwood). From the data in Table 1, it can be seen that the antibacterial effect of Comparative Example 3-4 is not only significantly lower than that of Example 3, but also simultaneously inhibits Staphylococcus epidermidis, indicating that replacing any component in the plant composition of the present invention with other raw materials with the same efficacy will affect the antibacterial activity of the composition against Staphylococcus aureus, Malassezia restricta and Staphylococcus epidermidis.

[0067] (2) Effect of plant composition on the activity of Malassezia restricta lipase Malassezia restricta is a skin commensal fungus that lacks the fatty acid synthase gene. The lipase it contains can degrade triglycerides in sebum. Triglycerides are hydrolyzed into unsaturated fatty acids such as oleic acid and arachidonic acid, which destroy the barrier function of the epidermal stratum corneum and cause various scalp problems.

[0068] The restrictive Malassezia lipase can hydrolyze p-nitrophenyl palmitate in vitro and release p-nitrophenol. The absorbance of p-nitrophenol in the culture supernatant is measured at a wavelength of 405 nm using a spectrophotometer to indicate the activity of the restrictive Malassezia lipase.

[0069] Table 2: Release of p-nitrophenol induced by Malassezia restricta From the data in Table 2, we can see that: The plant compositions of Examples 1-6 of the present invention can significantly reduce the lipase activity of Malassezia restricta, reduce its ability to utilize scalp lipids, and reduce the irritation of the unsaturated fatty acids produced by lipase hydrolysis to the scalp. Among them, Examples 1-4 have better effects than Examples 5-6.

[0070] The lipase inhibition effect of Comparative Examples 1-2 using any one component is significantly lower than that of Example 3. At the same time, the two raw materials in the plant composition of the present invention are replaced by currently recognized raw materials with similar efficacy. From the data in Table 2, it can be seen that the lipase inhibition effect of Comparative Examples 3-4 is significantly lower than that of Example 3, indicating that replacing any one component in the plant composition of the present invention with other raw materials with the same efficacy will reduce its ability to inhibit the activity of Malassezia restricta lipase.

[0071] (3) Cell wall integrity and alkaline phosphatase (AKP) content The cell wall is the first barrier between the cell and the outside world, isolating the cell from the outside world and protecting the cell from external harmful factors. In addition, the cell wall also plays a role in fixing the stability of the cell structure. Alkaline phosphatase (AKP) is a substance that exists between the cell membrane and the cell wall. It is usually used as one of the indicators to judge whether the cell wall is damaged. Because when the cell wall is damaged, the AKP in it loses the restraint of the cell wall, causing the AKP to leak. The amount of AKP in the bacterial suspension can be determined by detecting the absorbance of the bacterial suspension at a wavelength of 410nm, because the AKP content is proportional to the absorbance at this wavelength.

[0072] Add the sample to be tested to the activated bacterial suspension, and use sterile water as a blank control. Incubate at 30°C, and take 1 mL of sample after the sample is in contact with the bacterial suspension for 30 minutes. Centrifuge the above reaction solution at 4000rpm for 5 minutes, take 0.2 mL of the upper clear liquid and add it to 1.8 mL of 0.1 mol / L Tris-HCL buffer (pH 8.0, containing 0.2 mg / mL of disodium p-nitrophenylphosphate tetrahydrate) and mix well. The mixed solution is placed in a water bath at 23°C for 30 minutes. Measure the absorbance at OD410nm to indicate the content of alkaline phosphatase (AKP) in the solution. The test is repeated 3 times.

[0073] from Figure 1 It can be seen from the data that the absorbance of the bacterial suspension in the blank group at 410nm changes very little, indicating that the cell wall of Malassezia restricta in the blank group functions normally and the bacteria can grow normally. The absorbance of the bacterial suspension added with Examples 1-6 of the plant composition of the present invention at 410nm increases significantly, indicating that the addition of Examples 1-6 significantly destroys the integrity of the cell wall of Malassezia restricta, causing a large amount of alkaline phosphatase AKP in the cells to leak into the culture medium. In Comparative Examples 1-2 using any one of the components, the amount of AKP leakage is significantly lower than that of Examples 1-6. At the same time, any one of the two raw materials in the plant composition of the present invention is replaced with currently recognized raw materials with similar efficacy, Figure 1 It can be seen from the data that the intracellular AKP leakage is lower than that in the embodiment, indicating that replacing any component in the plant composition of the present invention with other raw materials with the same efficacy will reduce the ability to destroy the integrity of the cell wall of Malassezia restricta.

[0074] (4) Cell membrane permeability and intracellular nucleic acid leakage The cell membrane is one of the three major cell structures. The cell membrane is a physical barrier that protects intracellular substances from flowing out of the cell. The membrane is an important place for cell metabolism and growth and development. There are a large number of macromolecules such as proteins and nucleic acids. Once the cell membrane is damaged, macromolecules, including nucleic acids and proteins, will flow out of the cell, causing cell death. Therefore, detecting changes in the content of nucleic acids in bacterial suspensions can be used as an indicator to judge whether the cell membrane is damaged, and changes in the content of nucleic acids are positively correlated with changes in absorbance at a wavelength of 260nm. Therefore, the amount of intracellular nucleic acid leakage can be used to evaluate whether the permeability of the cell membrane has changed.

[0075] The activated bacterial suspension was washed three times with sterile phosphate buffer (0.1 mol / L, pH = 7.4), resuspended with sterile phosphate buffer, and then adjusted to about 2 × 10 5 CFU / mL. Add the test solution respectively, use sterile water as blank control, culture at 30℃ in a shaker, take out after 12h, and centrifuge at 4000rpm. Take 200μL of the upper supernatant in a 96-well ELISA plate and measure the OD value at 260nm. The treatment with sterile water was used as blank control, and the experiment was repeated 3 times.

[0076] from Figure 2 It can be seen from the data that the absorbance of the bacterial suspension in the blank group at 260nm changes very little, indicating that the restrictive Malassezia in the blank group grows normally. The absorbance of the bacterial suspension added with Examples 1-6 of the plant composition of the present invention at 260nm increases significantly, indicating that Examples 1-6 significantly destroy the permeability of the cell membrane of restrictive Malassezia, causing a large amount of leakage of intracellular nucleic acid. In contrast, the amount of leakage of intracellular nucleic acid in Comparative Examples 1-2 using any one of the components is significantly lower than that in Examples 1-6. At the same time, any one of the two raw materials in the plant composition of the present invention is replaced with currently recognized raw materials with similar efficacy, Figure 2 It can be seen from the data that the intracellular nucleic acid leakage is lower than that in the embodiment, indicating that replacing any component in the plant composition of the present invention with other raw materials with the same efficacy will reduce the degree of damage to the permeability of the cell membrane of Malassezia restricta.

[0077] Application Example 1: Preparation of antibacterial antidandruff shampoo Table 3: Antibacterial and anti-dandruff shampoo formula Preparation process: First disperse and swell phase A under cold water conditions, then heat to 80°C, add the components of phase B in sequence, add phase C to adjust the pH to 6.0-6.5, then cool to 45°C, add the components of phase E in sequence, and stir evenly.

[0078] Application Example 2 The difference between Application Example 2 and Application Example 1 is that the plant composition (Example 3) is replaced by Example 2, and the rest is the same.

[0079] Application Comparative Example 1-2 The difference between Application Comparative Examples 1-2 and Application Example 1 is that the plant composition (Example 3) is replaced by Comparative Examples 1-2 respectively, and the rest is the same.

[0080] (5) Human efficacy test Test samples: Application Example 1, Application Example 2, Application Comparative Example 1, Application Comparative Example 2, and commercially available anti-dandruff shampoo.

[0081] Test method: Fifty subjects aged between 18 and 50 years old with severe dandruff symptoms and without hair diseases such as psoriasis were selected, half of them were male and half were female, and randomly divided into 5 groups to try the shampoo of the above embodiment, comparative example and commercially available product respectively for 30 days, washing their hair every two days.

[0082] Test results: A questionnaire survey was conducted to evaluate the effectiveness of the shampoo subjectively based on the degree of improvement in the amount of dandruff, scalp itching, and scalp oil content before and after use. The subjects were scored on a 0-5 point scale, where 0 = no improvement; 1 point = little improvement; 2 points = slight improvement; 3 points = obvious improvement; 4 points = significant improvement; 5 points = complete improvement or basic recovery.

[0083] Table 4: Human efficacy tests Table 4 results show that the antibacterial anti-dandruff shampoo application examples 1-2 prepared by the plant composition of the present invention, after the volunteers tried it, filled in the questionnaire survey, and the result scores were all above 14 points, which was higher than the commercially available anti-dandruff shampoo, indicating that it can significantly improve the dandruff quantity, scalp itching degree and scalp oil secretion of the scalp. And the antibacterial anti-dandruff shampoo application comparative example 1-2 prepared using one of the components, the questionnaire survey result score after trial was between 11-12, which was significantly lower than the application example and the commercially available product, indicating that the plant composition of the present invention can be compounded in a specific ratio by two components of alpine fireweed and birch, and can synergize and significantly enhance the effect of targeted antibacterial, while inhibiting restrictive Malassezia and Staphylococcus aureus, it does not affect the normal proliferation of Staphylococcus epidermidis, and achieves the effect of comprehensive regulation of scalp microecology, thereby improving scalp problems such as dandruff, itchy head, and oily head.

[0084] Example 7 The present invention also studies the effects of different extraction methods on the extraction rates of alpine edelweiss and birch bark. In Example 7, the present invention adopts a multi-stage liquid phase pulse discharge extraction process, which is as follows: The dried plant materials of alpine edelweiss flowers and birch bark are crushed respectively, passed through a 10-mesh sieve, and the powders are mixed in a mass ratio of 2:1; 30% (volume ratio) ethanol aqueous solution (6 times the mass ratio) is added to the above mixture, and liquid phase pulse discharge extraction is performed, filtered, and the extract is concentrated under reduced pressure to an extract state and then freeze-dried to obtain a plant composition.

[0085] Among them, the liquid phase pulse discharge extraction adopts the following multi-stage differentiation procedure: (a) Discharge voltage 1KV, temperature 15℃, time 1min; (b) Discharge voltage 4KV, temperature 25℃, time 3min; (c) Discharge voltage 6KV, temperature 30℃, time 4.5min; (d) Discharge voltage 8KV, temperature 40℃, time 7min.

[0086] Table 5: Effect of different extraction processes on extraction rate Experimental Group Extraction rate (%) Example 3 21.3％ Example 7 25.9％ Extraction rate = extract mass / herbal material mass × 100%.

[0087] It can be seen from the data in Table 5 that: compared with Example 3, after the multi-stage liquid-phase pulse discharge extraction process of Example 7 was used to replace the conventional ultrasonic extraction process in Example 3, the extraction rate of the active ingredients in the final plant extract was significantly improved. Moreover, compared with the ultrasonic extraction process, the multi-stage liquid-phase pulse discharge extraction process requires less time, has higher extraction efficiency, and consumes less ethanol, and is therefore more worthy of promotion.

[0088] The raw materials and equipment used in the present invention, unless otherwise specified, are all commonly used raw materials and equipment in the art; the methods used in the present invention, unless otherwise specified, are all conventional methods in the art.

[0089] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A plant composition for regulating the microecological balance of the scalp, characterized in that: The invention comprises extracts of alpine edelweiss and birch bark; the extraction part of the alpine edelweiss is the alpine edelweiss flower and / or the alpine edelweiss leaf.

2. The plant composition according to claim 1, characterized in that: The mass ratio of the alpine edelweiss to the birch bark is 1:2 to 5:

1.

3. The plant composition according to claim 2, characterized in that: The mass ratio of the alpine edelweiss to the birch bark is 1:1 to 4:

1.

4. The plant composition according to any one of claims 1 to 3, characterized in that: The extract is an alcohol extract.

5. A method for preparing the plant composition according to any one of claims 1 to 4, characterized in that The following steps are involved: Crush the alpine edelweiss and birch bark separately, sieve and mix; The extraction solvent is an alcohol aqueous solution, ultrasonic extraction is performed, the extraction solution is filtered, concentrated, and dried.

6. The preparation method according to claim 5, characterized in that: The sieving is through a 10-40 mesh sieve; The alcohol is selected from one or more of ethanol, propylene glycol and butylene glycol; The mass ratio of the alcohol aqueous solution to the raw material is 5-15:1; the volume concentration of the alcohol aqueous solution is 40-60%; The conditions of the ultrasonic extraction are: ultrasonic power of 100-400W, ultrasonic temperature of 45-60°C, ultrasonic time of 20-50min, and ultrasonic times of 1-5 times; The concentration is concentrated to an extract form; The drying is freeze drying.

7. A method for preparing the plant composition according to any one of claims 1 to 4, characterized in that The following steps are involved: The alpine edelweiss and birch bark are crushed, sieved, mixed, added into an alcohol aqueous solution, subjected to liquid phase pulse discharge extraction, filtered, and the extract is concentrated and dried.

8. The preparation method according to claim 7, characterized in that: The sieving is through a 10-40 mesh sieve; The alcohol is selected from one or more of ethanol, propylene glycol and butylene glycol; The mass ratio of the alcohol aqueous solution to the raw material is 5-10:1; the volume concentration of the alcohol aqueous solution is 20-40%; The liquid phase pulse discharge extraction adopts a multi-stage differentiation procedure: (a) Discharge voltage 1-2KV, temperature 15-20℃, time 0.5-1.5min; (b) Discharge voltage 3-5KV, temperature 25-30℃, time 2-4min; (c) Discharge voltage 5-7KV, temperature 30-35℃, time 4-5min; (d) Discharge voltage 7-8KV, temperature 35-45℃, time 6-8min.

9. Use of the plant composition according to any one of claims 1 to 4 or the plant composition obtained by the preparation method according to any one of claims 5 to 8 in targeted inhibition of Staphylococcus aureus and Malassezia restricta.

10. Use of the plant composition according to any one of claims 1 to 4 or the plant composition obtained by the preparation method according to any one of claims 5 to 8 in cosmetics, characterized in that: The cosmetics are products that have the function of targeted inhibition of Staphylococcus aureus and Malassezia restricta, and do not affect Staphylococcus epidermidis.

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