Inducer for high yield of gibberellic acid and fermentation method thereof

Abstract

The invention discloses an inducer for high yield of gibberellic acid and a fermentation method of the inducer, and belongs to the technical field of plant growth regulators, the inducer comprises 5-10 parts of a Atripton squama extract, 0.5-1.2 parts of 2-naphthoxyacetic acid, 0.8-1.8 parts of p-chlorophenoxyacetic acid, and 0.8-1.2 parts of indole-3-butyric acid potassium. The fermentation method comprises the following steps: transferring a gibberellic acid seed solution into a fermentation culture medium, fermenting for 2-3 days, adding the inducer when the measured pH value is reduced to 4.8-4.9, and continuously fermenting for 4-5 days. By optimizing the fermentation method and improving the proportion of the inducer, the active regulation effect of gibberellic acid on wheat growth at a lower concentration is realized. Not only is the usage amount of gibberellic acid reduced, but also the cost related to the gibberellic acid is reduced, so that the overall economic benefit is improved. By combining the addition periods of the tambidium sphaeroides extract and the inducer, the synergistic effect of the tambidium sphaeroides extract and the inducer in promoting the stability of gibberellic acid is shown.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulators, and in particular to an inducer for high-yield gibberellic acid and a fermentation method thereof. Background Art

[0002] Gibberellic acid (GAs) is a plant growth regulator widely used in agriculture. It has multiple biological activities such as promoting plant cell division, elongation and differentiation, and can significantly improve crop yield and quality.

[0003] Existing gibberellic acid preparations have some problems in practical application, which limits their wider application.

[0004] First of all, gibberellic acid usually requires a higher concentration to achieve the ideal growth regulation effect, which not only increases the cost of use, but may also cause excessive plant growth, leggy growth and other undesirable phenomena.

[0005] Secondly, gibberellic acid preparations have poor stability and are easily decomposed under high temperature, light or acid-base conditions, which requires special attention to environmental conditions during storage and transportation, increasing the cost and difficulty of storage and transportation.

[0006] Based on this, the present invention designs an inducer for high production of gibberellic acid and a fermentation method thereof to solve the above problems. Summary of the invention

[0007] In view of the above-mentioned shortcomings of the prior art, the present invention provides an inducer for high-yield gibberellic acid and a fermentation method thereof.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: An inducer for high-yield gibberellic acid, comprising the following raw materials: 5-10 parts of scaly leaf moss extract, 0.5-1.2 parts of 2-naphthoxyacetic acid, 0.8-1.8 parts of p-chlorophenoxyacetic acid, 0.8-1.2 parts of potassium indolebutyrate; The preparation method of the inducer comprises the following steps: Step 1: Weigh the powder of 120-200 meshes of Lepidium melongena powder and put it into a centrifuge tube, add ethanol solution thereto, shake and centrifuge, take the supernatant and dry it to constant weight to obtain the primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 4.5-5.2 and the temperature to 30-32° C., and add potassium indolebutyrate to the primary extract in batches to obtain a composite solution A; Step 3: Fully mix 2-naphthoxyacetic acid and p-chlorophenoxyacetic acid and dissolve them in ethanol solution to obtain a mixed acid solution, gradually add the compound solution A dropwise to the mixed acid solution, and shake to obtain the inducer.

[0009] Preferably, step one is specifically as follows: weigh 10-20 parts of scaly moss powder and put it into a centrifuge tube, add 50-100 parts of 90% ethanol solution thereto, shake for 20-30 minutes, and then centrifuge at 1800-2000r / min for 15-20 minutes, take the supernatant and dry it in a 60°C oven to constant weight, to obtain 5-10 parts of primary extract.

[0010] Preferably, step 2 is specifically as follows: hydrochloric acid is added dropwise to the primary extract to adjust the pH value to 4.5-5.2 and the temperature to 30-32° C., and 0.8-1.2 parts of potassium indolebutyrate are added to the primary extract three times to obtain a composite solution A.

[0011] Preferably, in step 2, 0.8-1.2 parts of potassium indolebutyrate are added to the primary extract three times with an interval of 10-15 minutes between each addition, and the temperature is raised by 3-5° C. after each addition while stirring at a speed of 200-300 r / min.

[0012] Preferably, step three is specifically as follows: 0.5-1.2 parts of 2-naphthoxyacetic acid and 0.8-1.8 parts of p-chlorophenoxyacetic acid are fully mixed and dissolved in 20-40 parts of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A is gradually added dropwise to the mixed acid solution, and the inducer is obtained after shaking for 5-8 minutes.

[0013] Preferably, the compound liquid A is gradually added to the mixed acid solution at a rate of 6-8 drops / min.

[0014] Preferably, the particle size of the scaly moss powder is 120-200 meshes.

[0015] A fermentation method for high-yield gibberellic acid, according to the inducer for high-yield gibberellic acid, the inducer is applied to the fermentation method for high-yield gibberellic acid, comprising the following steps: Transfer the gibberellic acid seed liquid into the fermentation medium at 32-35°C, pH 5.1-5.3, pressure 0.03-0.06MPa, air flow 1000-2000Nm 3 / h for 2-3 days, and when the pH value is measured to be reduced to 4.8-4.9, the inducer is added; At 35-38℃, pH value is 4.8-4.9, pressure is 0.05-0.07MPa, air flow rate is 1800-2500Nm 3 / h and continue fermentation for 4-5 days.

[0016] Preferably, the mass ratio of the inducer to the gibberellic acid seed solution is 1-2:15-20, and the fermentation medium includes the following components: 50-60 g / L corn gluten meal, 1.8-5.6 g / L potassium dihydrogen phosphate, 40-80 g / L glucose, 0.2-0.4 g / L olive oil, 2-8 g / L sodium sulfate, 0.02-0.05 g / L nickel nitrate and 0.02-0.05 g / L calcium bicarbonate.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention has determined through experimental research that the optimal concentration of gibberellic acid in promoting wheat growth is 8 mg / L. This discovery has significant advantages over the optimal concentration of gibberellic acid of about 15 mg / L commonly used in the prior art. By optimizing the fermentation method and improving the ratio of the inducer, the positive regulatory effect of gibberellic acid on wheat growth at a lower concentration is achieved. In the comparative experiments of the embodiments and the comparative examples, it is observed that at a gibberellic acid concentration of 8 mg / L, the growth rates of the stem height, fresh weight and dry weight of wheat are generally high. This phenomenon shows that a gibberellic acid concentration of 8 mg / L can effectively promote the growth of wheat without using a higher concentration of gibberellic acid. This optimization not only reduces the amount of gibberellic acid used, but also reduces the costs associated therewith, thereby improving the overall economic benefits.

[0018] 2. The present invention helps to reduce the risk of plant toxicity caused by high concentrations of gibberellic acid by achieving the growth regulation effect of gibberellic acid at a lower concentration. This low concentration usage is not only safer for plant growth, but also more environmentally friendly.

[0019] 3. The present invention investigated the effect of the addition cycle of the scaly leaf moss extract or the inducer on the stability of gibberellic acid. The results showed that when these two components were used independently, they failed to significantly enhance the tolerance of gibberellic acid under extreme environmental conditions, such as high temperature heating or high-intensity light. By combining the addition cycle of the scaly leaf moss extract with the inducer, the synergistic effect of the two in promoting the stability of gibberellic acid was demonstrated. This combination not only significantly improves the aging resistance of gibberellic acid under heating conditions, enabling it to maintain activity at higher temperatures and reduce degradation, but also greatly enhances its stability under strong light conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 This is a curve diagram of stem height changing with gibberellic acid concentration; Figure 2 This is the curve of fresh weight changing with gibberellic acid concentration; Figure 3 This is a curve showing the change of dry weight with gibberellic acid concentration. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Embodiment 1: This embodiment provides a fermentation method for high-yield gibberellic acid, comprising the following steps: Step 1: Weigh 20 g of 200-mesh scaly moss powder into a centrifuge tube, add 100 mL of 90% ethanol solution, shake for 30 min, and centrifuge at 2000 r / min for 20 min. Take the supernatant and dry it in a 60°C oven to constant weight to obtain 10 g of primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 5.2 and the temperature to 32°C, add 1.2 g of potassium indolebutyrate to the primary extract three times to obtain a composite solution A, with an interval of 15 minutes each time, and increase the temperature by 5°C after each addition, while stirring at a speed of 300 r / min; Step 3: 1.2 g of 2-naphthoxyacetic acid and 1.8 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 40 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 8 drops / min, and the inducer was obtained after shaking for 8 minutes; Step 4: Transfer the gibberellic acid seed solution into the fermentation medium (the fermentation medium includes the following components: 60g / L corn protein powder, 5.6g / L potassium dihydrogen phosphate, 80g / L glucose, 0.4g / L olive oil, 8g / L sodium sulfate, 0.05g / L nickel nitrate and 0.05g / L calcium bicarbonate) at 35°C, pH 5.3, pressure 0.06MPa, air flow 2000Nm 3 / h for 3 days, and when the pH value is measured to be reduced to 4.9, the inducer is added, and the mass ratio of the inducer to the gibberellic acid seed liquid is 2:20; Step 5: At 38°C, pH 4.9, pressure 0.07MPa, air flow 2500Nm 3 / h, and continued fermentation for 5 days to obtain gibberellic acid.

[0024] Embodiment 2: This embodiment provides a fermentation method for high-yield gibberellic acid, comprising the following steps: Step 1: Weigh 10 g of 120-mesh scaly moss powder into a centrifuge tube, add 50 mL of 90% ethanol solution, shake for 20 min, centrifuge at 1800 r / min for 15 min, take the supernatant and dry it in a 60°C oven to constant weight to obtain 5 g of primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 4.5 and the temperature to 30°C, add 0.8 g of potassium indolebutyrate to the primary extract three times to obtain a composite solution A, with an interval of 10 minutes each time, and increase the temperature by 3°C after each addition, while stirring at a speed of 200 r / min; Step 3: 0.5 g of 2-naphthyloxyacetic acid and 0.8 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 20 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 6 drops / min, and the inducer was obtained after shaking for 5 minutes; Step 4: Transfer the gibberellic acid seed solution into the fermentation medium (the fermentation medium includes the following components: 50 g / L corn protein powder, 1.8 g / L potassium dihydrogen phosphate, 40 g / L glucose, 0.2 g / L olive oil, 2 g / L sodium sulfate, 0.02 g / L nickel nitrate and 0.02 g / L calcium bicarbonate) at 32 ° C, pH 5.1, pressure 0.03 MPa, air flow 1000 Nm 3 / h for 2 days, and when the pH value is measured to be reduced to 4.8, the inducer is added, and the mass ratio of the inducer to the gibberellic acid seed liquid is 1:15; Step 5: At 35°C, pH 4.8, pressure 0.05MPa, air flow 1800Nm 3 / h, and fermentation was continued for 4 days to obtain gibberellic acid.

[0025] Embodiment 3: This embodiment provides a fermentation method for high-yield gibberellic acid, comprising the following steps: Step 1: Weigh 12 g of 140-mesh scaly moss powder into a centrifuge tube, add 80 mL of 90% ethanol solution, shake for 25 min, centrifuge at 1900 r / min for 17 min, take the supernatant and dry it in a 60°C oven to constant weight to obtain 8 g of primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 4.9 and the temperature to 31°C, add 1.1 g of potassium indolebutyrate to the primary extract three times to obtain a composite solution A, each time with an interval of 14 minutes, raise the temperature by 4°C after each addition, and stir at a speed of 260 r / min; Step 3: 0.8 g of 2-naphthoxyacetic acid and 1.4 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 35 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 7 drops / min, and the inducer was obtained after shaking for 7 minutes; Step 4: Transfer the gibberellic acid seed solution into the fermentation medium (the fermentation medium includes the following components: 55g / L corn protein powder, 4.2g / L potassium dihydrogen phosphate, 62g / L glucose, 0.3g / L olive oil, 4g / L sodium sulfate, 0.03g / L nickel nitrate and 0.04g / L calcium bicarbonate) at 34°C, pH 5.2, pressure 0.05MPa, air flow 1800Nm 3 / h for 2 days, and when the pH value is measured to be reduced to 4.8, the inducer is added, and the mass ratio of the inducer to the gibberellic acid seed liquid is 1.5:17; Step 5: At 37°C, pH 4.8, pressure 0.06MPa, air flow 2000Nm 3 / h, and continued fermentation for 5 days to obtain gibberellic acid.

[0026] Comparative Example 1: The difference from Example 3 is that the inducer in this comparative example does not contain the extract of Lepidium melongena; This comparative example provides a fermentation method for high-yield gibberellic acid, comprising the following steps: Step 1: Add hydrochloric acid dropwise to 8 g of distilled water to adjust the pH value to 4.9 and the temperature to 31°C, add 1.1 g of potassium indolebutyrate to the hydrochloric acid solution three times, each time with an interval of 14 minutes, raise the temperature by 4°C after each addition, and stir at a speed of 260 r / min; Step 2: 0.8 g of 2-naphthoxyacetic acid and 1.4 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 35 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 7 drops / min, and the inducer was obtained after shaking for 7 minutes; Step 3: Transfer the gibberellic acid seed solution into the fermentation medium (the fermentation medium includes the following components: 55g / L corn protein powder, 4.2g / L potassium dihydrogen phosphate, 62g / L glucose, 0.3g / L olive oil, 4g / L sodium sulfate, 0.03g / L nickel nitrate and 0.04g / L calcium bicarbonate) at 34°C, pH 5.2, pressure 0.05MPa, air flow 1800Nm 3 / h for 2 days, and when the pH value is measured to be reduced to 4.8, the inducer is added, and the mass ratio of the inducer to the gibberellic acid seed liquid is 1.5:17; Step 4: At 37°C, pH 4.8, pressure 0.06MPa, air flow 2000Nm3 / h, and continued fermentation for 5 days to obtain gibberellic acid.

[0027] Comparative Example 2: The difference from Example 3 is that the inducer is added to the fermentation medium before starting the fermentation; This comparative example provides a fermentation method for high-yield gibberellic acid, comprising the following steps: Step 1: Weigh 12 g of 140-mesh scaly moss powder into a centrifuge tube, add 80 mL of 90% ethanol solution, shake for 25 min, centrifuge at 1900 r / min for 17 min, take the supernatant and dry it in a 60°C oven to constant weight to obtain 8 g of primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 4.9 and the temperature to 31°C, add 1.1 g of potassium indolebutyrate to the primary extract three times to obtain a composite solution A, each time with an interval of 14 minutes, raise the temperature by 4°C after each addition, and stir at a speed of 260 r / min; Step 3: 0.8 g of 2-naphthoxyacetic acid and 1.4 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 35 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 7 drops / min, and the inducer was obtained after shaking for 7 minutes; Step 4: Transfer the gibberellic acid seed solution into a fermentation medium (the fermentation medium includes the following components: 55 g / L corn protein powder, 4.2 g / L potassium dihydrogen phosphate, 62 g / L glucose, 0.3 g / L olive oil, 4 g / L sodium sulfate, 0.03 g / L nickel nitrate and 0.04 g / L calcium bicarbonate), add the inducer, the mass ratio of the inducer to the gibberellic acid seed solution is 1.5:17, at 34°C, pH 5.2, pressure 0.05 MPa, air flow 1800 Nm 3 / h for 2 days; Step 5: When the pH value is measured to be 4.8, at 37°C, pH value is 4.8, pressure is 0.06MPa, and air flow rate is 2000Nm 3 / h, and continued fermentation for 5 days to obtain gibberellic acid.

[0028] Comparative Example 3: The difference from Example 3 is that the inducer in this comparative example does not contain the scaly leaf moss extract, and the inducer is added to the fermentation medium before starting the fermentation; Step 1: Add hydrochloric acid dropwise to 8 g of distilled water to adjust the pH value to 4.9 and the temperature to 31°C, add 1.1 g of potassium indolebutyrate to the hydrochloric acid solution three times, each time with an interval of 14 minutes, raise the temperature by 4°C after each addition, and stir at a speed of 260 r / min; Step 2: 0.8 g of 2-naphthoxyacetic acid and 1.4 g of p-chlorophenoxyacetic acid were fully mixed and dissolved in 35 mL of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A was gradually added dropwise to the mixed acid solution at a rate of 7 drops / min, and the inducer was obtained after shaking for 7 minutes; Step 3: Transfer the gibberellic acid seed solution into a fermentation medium (the fermentation medium includes the following components: 55 g / L corn protein powder, 4.2 g / L potassium dihydrogen phosphate, 62 g / L glucose, 0.3 g / L olive oil, 4 g / L sodium sulfate, 0.03 g / L nickel nitrate and 0.04 g / L calcium bicarbonate), add the inducer, the mass ratio of the inducer to the gibberellic acid seed solution is 1.5:17, at 34°C, pH 5.2, pressure 0.05 MPa, air flow 1800 Nm 3 / h for 2 days; Step 4: When the pH value is measured to be 4.8, at 37°C, pH value is 4.8, pressure is 0.06MPa, and air flow rate is 2000Nm 3 / h, and continued fermentation for 5 days to obtain gibberellic acid.

[0029] Experimental Example 1: Indoor bioassay of gibberellic acid; The plant seeds used in this experiment were wheat (Malan 1, Xinong 511, Bainong 4199, and Xinmai 26), of which the four wheat varieties were used in equal quantities and the final value was taken as the average; Each group of gibberellic acid (Examples 1-3 and Comparative Examples 1-3) was diluted to four concentrations of 4 mg / L, 8 mg / L, 12 mg / L, and 16 mg / L for testing.

[0030] Select 20 wheat seeds of uniform size, fullness and color, and sow them in a plastic seedling pot with an outer diameter of 95mm and a height of 82mm. When sowing, ensure that each seed is kept at an appropriate distance and covered with 1.1cm of soil. Then, irrigate the seedling pot filled with a mixture of nutrient soil and perlite (ratio of 1.5:1.2) with tap water. Finally, place the seedling pot in a climate chamber set at a temperature of 26°C, a light intensity of 10,000lx, and a photoperiod of 12h / 12h; In the climate chamber, wheat seedlings were thinned out at the seedling stage, and 12 wheat seedlings of basically the same size and growth were retained in each pot. Subsequently, 210 mL of compound solutions with different concentration gradients were used to irrigate the roots of these wheat seedlings. Three parallel experiments were set for each concentration, and clean water was used as the control group; After 7 days of cultivation in the climate chamber, the wheat plants were taken out of the seedling pots, the soil on the roots was washed with clean water, and the surface moisture was wiped with a paper towel, and then the stem height and fresh weight of the plants were measured. Finally, the plants were placed in an oven, first at 105°C for 20 minutes, and then dried at 75°C to constant weight to measure the dry weight of the plants.

[0031] (1) Effect on seed stem growth: Effect of drug on stem growth = (stem height of drug-treated plants - stem height of water-treated plants) / stem height of water-treated plants × 100%.

[0032] (2) Effects on plant dry weight and fresh weight: Effect on seedling fresh weight = (fresh weight of plants treated with pesticides - fresh weight of plants treated with water) / fresh weight of plants treated with water × 100%; Effect on seedling dry weight = (dry weight of the plant treated with the drug - dry weight of the plant treated with water) / fresh weight of the plant treated with water × 100%; The fresh weight data of a single seedling mainly reflects the overall growth of wheat, including roots, stems, and wheat cotyledons; The higher the fresh weight, the more active the cell life activities, the faster the cell division rate, and the more vigorous the wheat growth; The dry weight data of a single seedling usually reflects the health status and growth ability of the plant. It is directly related to the organic matter accumulation and growth vitality of the plant. The higher the dry weight, the stronger the nutrient absorption capacity of the plant and the more dry matter accumulation. The statistical results are shown in Table 1.

[0033] Table 1: Effects of gibberellic acid on wheat growth

[0034] The data in the above table can be made into a line chart, such as Figure 1-3 As shown; Combining the above table with Figure 1-3 It can be seen that: Stem height: At a concentration of 8 mg / L, the growth rate of stem height was generally high, especially in Example 3, which reached 19.21%. At a concentration of 16 mg / L, the growth rate of stem height was generally negative, indicating that excessive gibberellic acid concentration had an inhibitory effect on stem height; Fresh weight: Also at a concentration of 8 mg / L, the fresh weight growth rate was generally high, reaching 45.43% and 36.52% in Example 1 and Example 3, respectively. At a concentration of 16 mg / L, the fresh weight growth rate was generally negative, indicating that excessively high concentrations also had an inhibitory effect on fresh weight; Dry weight: As the concentration of gibberellic acid increases, the dry weight increases accordingly, indicating that as the concentration of gibberellic acid increases, the nutrient absorption capacity of wheat becomes stronger and the dry matter accumulation becomes more.

[0035] Optimum concentration: From the data, a gibberellic acid concentration of 8 mg / L seems to be the optimal concentration for promoting wheat growth. Regardless of the example or the comparative example, the growth rates of stem height, fresh weight and dry weight are generally higher at this concentration. Compared with the optimal concentration of gibberellic acid in the prior art (about 15 mg / L), the present invention optimizes the fermentation method and improves the ratio of the inducer, thereby achieving the growth regulating effect of gibberellic acid at a low concentration (8 mg / L), reducing the cost of use and improving the economic benefits.

[0036] Experimental Example 2: Stability test of gibberellic acid; The gibberellic acid of different concentrations (4 mg / L, 8 mg / L, 12 mg / L, 16 mg / L) in Example 1 and Comparative Examples 1-3 were placed at 60°C and 10000 lx light intensity for 120 h for aging experiment, and then the stem height, fresh weight and dry weight were detected by the method of Experimental Example 1, and the standard deviation was calculated: Standard deviation = | (value after aging - value before aging) | / value before aging; The statistical results are shown in Table 2: Table 2: Effects of gibberellic acid on wheat growth before and after aging

[0037] It can be seen from the above table that there is no obvious change between Comparative Examples 1-2 and Example 1, that is, the addition cycle of the scaly moss extract and the inducer will not have a significant effect on the anti-aging effect of gibberellic acid, but the combined use of the two can significantly improve the anti-aging effect of gibberellic acid under heating and high-intensity light conditions.

[0038] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An inducer for high-yield gibberellic acid, characterized in that: Including the following ingredients: 5-10 parts of scaly leaf moss extract, 0.5-1.2 parts of 2-naphthoxyacetic acid, 0.8-1.8 parts of p-chlorophenoxyacetic acid, 0.8-1.2 parts of potassium indolebutyrate; The preparation method of the inducer comprises the following steps: Step 1: Weigh the powder of 120-200 meshes of Lepidium melongena powder and put it into a centrifuge tube, add ethanol solution thereto, shake and centrifuge, take the supernatant and dry it to constant weight to obtain the primary extract; Step 2: Add hydrochloric acid dropwise to the primary extract to adjust the pH value to 4.5-5.2 and the temperature to 30-32° C., and add potassium indolebutyrate to the primary extract in batches to obtain a composite solution A; Step 3: Fully mix 2-naphthoxyacetic acid and p-chlorophenoxyacetic acid and dissolve them in ethanol solution to obtain a mixed acid solution, gradually add the compound solution A dropwise to the mixed acid solution, and shake to obtain the inducer.

2. The inducer for high-yield gibberellic acid according to claim 1, characterized in that Step one is specifically as follows: weigh 10-20 parts of scaly moss powder and put it into a centrifuge tube, add 50-100 parts of 90% ethanol solution thereto, shake for 20-30 minutes, and then centrifuge at 1800-2000r / min for 15-20 minutes, take the supernatant and dry it in a 60°C oven to constant weight, and obtain 5-10 parts of primary extract.

3. The inducer for high-yield gibberellic acid according to claim 2, characterized in that Step 2 is specifically as follows: hydrochloric acid is added dropwise to the primary extract to adjust the pH value to 4.5-5.2 and the temperature to 30-32° C., and 0.8-1.2 parts of potassium indolebutyrate are added to the primary extract three times to obtain a composite solution A.

4. The inducer for high-yield gibberellic acid according to claim 3, characterized in that In the step 2, 0.8-1.2 parts of potassium indolebutyrate are added to the primary extract three times, each time interval is 10-15 minutes, and the temperature is increased by 3-5° C. after each addition, while stirring at a speed of 200-300 r / min.

5. The inducer for high-yield gibberellic acid according to claim 4, characterized in that Step three is as follows: 0.5-1.2 parts of 2-naphthoxyacetic acid and 0.8-1.8 parts of p-chlorophenoxyacetic acid are fully mixed and dissolved in 20-40 parts of 90% ethanol solution to obtain a mixed acid solution, and the compound solution A is gradually added dropwise to the mixed acid solution, and the inducer is obtained after shaking for 5-8 minutes.

6. The inducer for high production of gibberellic acid according to claim 5, characterized in that The compound liquid A was gradually added to the mixed acid solution at a rate of 6-8 drops / min.

7. The inducer for high-yield gibberellic acid according to claim 6, characterized in that The particle size of the scaly moss powder is 120-200 mesh.

8. A fermentation method for high-yield gibberellic acid, characterized in that: According to any one of claims 1 to 7, the inducer for high-yield gibberellic acid is applied to a fermentation method for high-yield gibberellic acid, comprising the following steps: Transfer the gibberellic acid seed liquid into the fermentation medium at 32-35°C, pH 5.1-5.3, pressure 0.03-0.06MPa, air flow 1000-2000Nm 3 / h for 2-3 days, and when the pH value is measured to be reduced to 4.8-4.9, the inducer is added; At 35-38℃, pH value is 4.8-4.9, pressure is 0.05-0.07MPa, air flow rate is 1800-2500Nm 3 / h and continue fermentation for 4-5 days.

9. The fermentation method for high-yield gibberellic acid according to claim 8, characterized in that: The mass ratio of the inducer to the gibberellic acid seed solution is 1-2:15-20.

10. The fermentation method for high-yield gibberellic acid according to claim 9, characterized in that: The fermentation medium comprises the following components: 50-60 g / L corn gluten meal, 1.8-5.6 g / L potassium dihydrogen phosphate, 40-80 g / L glucose, 0.2-0.4 g / L olive oil, 2-8 g / L sodium sulfate, 0.02-0.05 g / L nickel nitrate and 0.02-0.05 g / L calcium bicarbonate.

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