Foliar spray for foxtail millet as well as preparation method and application of foliar spray

Abstract

The invention discloses a foxtail millet foliar spray as well as a preparation method and application thereof, and the foxtail millet foliar spray comprises the following components in parts by mass: 15-18 parts of jasmonic acid methyl ester, 6-9 parts of salicylic acid and 190-210 parts of a surfactant. The preparation method comprises the following steps: weighing raw materials methyl ester, salicylic acid and a surfactant in proportion, and uniformly mixing; and diluting with water before use. By applying the plant hormone methyl jasmonate and the plant growth regulator salicylic acid and compounding with the surfactant, the quality of the millet is improved. The foxtail millet foliar spray is sprayed after foxtail millet grains are formed in the grain filling stage, compared with unsprayed foxtail millet, the xanthophyll content, the yellow pigment content and the total flavone content of hulled foxtail millet are increased, the gelatinization characteristic of the foxtail millet flour is improved, and the quality of the foxtail millet is integrally improved.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a foliar fertilizer spray for improving the quality of millet after husking, and a preparation method and application thereof. Background Art

[0002] Millet has strong resistance to barrenness and drought, is widely planted in dryland agricultural ecological zones, and has become a strategic reserve crop to ensure food security.

[0003] Millet after shelling is rich in functional nutrients, such as lutein, xanthophyll, total flavonoids, etc., and has unique advantages in balancing the nutritional structure of residents' diet.

[0004] Therefore, how to further increase the content of the above nutrients is of great significance to the overall improvement of millet quality. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a millet foliar spray and a preparation method and application thereof, so as to increase the lutein content, yellow pigment content, total flavonoid content and gelatinization characteristics of millet after husking.

[0006] To solve the above technical problems, according to one aspect of the present invention, a millet foliar spray is provided, which comprises 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid and 190-210 parts of a surfactant, calculated by weight.

[0007] Furthermore, the invention comprises, by weight, 16 parts of methyl jasmonate, 8 parts of salicylic acid and 200 parts of a surfactant.

[0008] Furthermore, the surfactant is polyethylene glycol octylphenyl ether (Triton X-100).

[0009] According to another aspect of the present invention, a method for preparing the millet foliar spray is provided, wherein the raw materials of acid methyl ester, salicylic acid and surfactant are weighed in proportion and mixed evenly; and diluted with water before use.

[0010] Furthermore, the dilution factor was 2×10 5 times.

[0011] According to another aspect of the present invention, provided is the use of the above-mentioned millet foliar spray in improving the lutein content, yellow pigment content, total flavonoids content and gelatinization characteristics of millet.

[0012] Furthermore, the millet foliar spray is sprayed on the leaves after the grains are formed during the millet filling stage.

[0013] Furthermore, the spraying times are 3 to 4 times.

[0014] The invention improves the quality of millet by applying plant hormone methyl jasmonate and plant growth regulator salicylic acid and compounding them with surfactant.

[0015] By spraying the millet foliar spray of the present invention after the grains of millet are formed during the grain filling stage, the lutein content, yellow pigment content and total flavonoid content of the millet after shelling are increased compared with the millet without spraying, and the gelatinization characteristics of the millet flour are improved, thereby improving the overall quality of the millet. DETAILED DESCRIPTION

[0016] A typical embodiment of the present invention provides a millet foliar spray, which comprises, by weight, 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid, and 190-210 parts of a surfactant.

[0017] Among them, methyl jasmonate is a plant hormone and salicylic acid is a plant growth regulator. The addition of a surfactant increases the emulsification ability between the components in the spray, so that they can be more effectively mixed with each other, can adhere to the leaf surface, reduce the flow and release speed of the spray, increase the expansion area of ​​the spray, and increase the effect of foliar application. The surfactant is preferably polyethylene glycol octyl phenyl ether (Triton X-100).

[0018] When preparing the millet foliar spray, the raw materials of acid methyl ester, salicylic acid and surfactant are weighed in proportion and the above raw materials are mixed evenly. Dilute with water before use, and the preferred dilution multiple is 2×10 5 times.

[0019] After the grains of millet are formed during the grain filling stage, the millet foliar spray is sprayed on the leaves, and the preferred spraying frequency is 3 to 4 times.

[0020] The technical scheme claimed in the present invention is further described below by some examples. However, the examples and comparative examples are used to explain the embodiments of the present invention and do not exceed the scope of the subject matter of the present invention. The protection scope of the present invention is not limited by the examples. Unless otherwise specified, the materials and reagents used in the present invention can be obtained from commercial products in the art.

[0021] The methyl jasmonate, salicylic acid and surfactant Triton X-100 (polyethylene glycol octylphenyl ether) used in the following examples were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. Example 1

[0022] Planting Datong 29. The raw materials of the foliar spray are: 16kg of methyl jasmonate, 8kg of salicylic acid and 200kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5Spray 4 times after the grains are formed in the millet filling stage, spray 0.5 L per square meter. Example 2

[0023] Planting Datong 29. The raw materials of the foliar spray are: 15kg of methyl jasmonate, 6kg of salicylic acid and 210kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Spray 3 times during the millet filling stage after the grains are formed, spraying 0.5 L per square meter. Example 3

[0024] Planting Datong 29. The raw materials of the foliar spray are: 17kg of methyl jasmonate, 7kg of salicylic acid and 190kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Spray 4 times after the grains are formed in the millet filling stage, spray 0.5 L per square meter.

[0025] Comparative Example 1 Plant Datong 29. Spray the leaves of millet with distilled water 4 times without adding any reagents after grain formation at the grain filling stage, with a spraying rate of 0.5 L per square meter.

[0026] Comparative Example 2 The only difference from Example 1 is that the 6×10 5 times.

[0027] Comparative Example 3 The only difference from Example 1 is that the raw material composition is: 16 kg of methyl jasmonate and 200 kg of surfactant are mixed evenly, and salicylic acid is not included.

[0028] Comparative Example 4 The only difference from Example 1 is that the raw material composition is: 8 kg of salicylic acid and 200 kg of surfactant are mixed evenly, and methyl jasmonate is not included. Example 4

[0029] Planting Jingu 21. The raw materials of the foliar spray are: 16kg of methyl jasmonate, 8kg of salicylic acid and 200kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Spray 4 times after the grains are formed in the millet filling stage, spray 0.5 L per square meter. Example 5

[0030] Planting Jingu 21. The raw materials for the foliar spray are: 18 kg of methyl jasmonate, 9 kg of salicylic acid and 205 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Spray 3 times during the millet filling stage after the grains are formed, spray 0.5 L per square meter. Example 6

[0031] Planting Jingu 21. The raw materials for the foliar spray are: 16 kg of methyl jasmonate, 8 kg of salicylic acid and 195 kg of surfactant. The above raw materials are mixed evenly and diluted 2×10 before use. 5 Spray 4 times after the grains are formed in the millet filling stage, spray 0.5 L per square meter.

[0032] Comparative Example 5 Plant Jingu 21. Spray the millet with distilled water 4 times without adding any reagents after the grains are formed during the grain filling stage, spraying 0.5 L per square meter.

[0033] Comparative Example 6 The only difference from Example 4 is that the 6×10 5 times.

[0034] Comparative Example 7 The only difference from Example 4 is that the raw material composition is: 16 kg of methyl jasmonate and 200 kg of surfactant are mixed evenly, and salicylic acid is not included.

[0035] Comparative Example 8 The only difference from Example 5 is that the raw material composition is: 8 kg of salicylic acid and 200 kg of surfactant are mixed evenly, and methyl jasmonate is not included.

[0036] The effects of the fertilizers prepared in Examples 1 to 8 and Comparative Examples 1-2 were verified as follows: Experimental location: Dongyang Experimental Base of Shanxi Agricultural University (37°33′21″N, 112°40′2″E, 800 m above sea level).

[0037] Experimental crops: millet (variety Jingu 21 and Datong 29).

[0038] Planting area: Each treatment area was sprayed with foliar sprays of Examples 1-8 and Comparative Examples 1-2, respectively. Three replicate test plots were set up in completely randomized block arrangement. Each test plot was 6 m long and 5 m wide. Fertilization and daily management were carried out according to local practices.

[0039] The mature millet was shelled and the lutein content, zeaxanthin content, xanthophyll content, total flavonoid content and total polyphenol content in the millet were determined. The results are shown in Table 1.

[0040] Table 1 Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 1 Example 4 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 5 Lutein 8.90 7.90 7.65 7.30 7.25 29.78 26.88 26.58 25.28 25.24 Zeaxanthin 2.98 2.91 2.62 2.35 2.26 8.75 8.28 8.31 8.21 8.28 Yellow pigment (μg / g) 7.76 6.82 6.83 6.78 6.81 20.10 18.51 18.75 18.48 18.52 Total flavonoids (mg / 100g) 67.90 74.77 58.65 52.36 48.43 89.26 91.62 86.26 82.35 77.95 Total polyphenols (mg / 100g) 48.75 45.03 44.23 44.08 44.11 56.98 51.25 50.48 50.52 50.20 It can be seen from Table 1 that, compared with Comparative Example 1 and Comparative Example 5 in which no foliar spray was applied, the lutein content, xanthophyll content, total flavonoid content and total polyphenol content in millet were significantly increased after the foliar spray described in Example 1 and Example 4 of the present invention was applied.

[0041] Compared with Comparative Examples 2 and 6 in which foliar sprays of lower concentrations were applied, after applying the foliar sprays described in Examples 1 and 4 of the present invention, except for a slight decrease in the total flavonoids content in millet, the lutein content, xanthophyll content and total polyphenol content in millet were significantly increased.

[0042] Compared with Comparative Example 3, Comparative Example 4, Comparative Example 7 and Comparative Example 8 which do not contain methyl jasmonate or salicylic acid, after applying the foliar spray described in Example 1 and Example 4 of the present invention, the lutein content, yellow pigment content, total flavonoid content and total polyphenol content in millet are significantly increased.

[0043] The mature millet was husked and the cooking quality of the millet was determined using the gelatinization characteristic value as an indicator. The results are shown in Table 2.

[0044] Table 2 Gelatinization characteristics Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 1 Example 4 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 5 Gelatinization start time (min) 9.76 10.03 9.89 9.82 10.16 10.4 10.2 10.1 10.2 9.96 Gelatinization temperature (℃) 78.5 79.4 78.4 79.2 79.5 80.6 79.8 79.4 79.5 79.1 Peak viscosity (BU) 158 161 163 160 162 222 211 208 205 202 Final viscosity (BU) 283 278 276 274 275 452 442 438 435 430 Disintegration value (BU) 40 41 45 41 42 55 48 47 43 41 Recovery value (BU) 165 158 158 155 155 285 279 277 273 269 Peak-to-Valley Viscosity (BU) 118 120 118 119 120 167 163 161 162 161 It can be seen from Table 2 that, compared with Comparative Example 1 and Comparative Example 5 in which no foliar spray was applied, the application of the foliar spray described in Example 1 and Example 4 of the present invention significantly increased the final viscosity and recovery value of millet gelatinization.

[0045] Compared with Comparative Examples 2 and 6 in which foliar sprays of lower concentrations were applied, the application of the foliar sprays of Examples 1 and 4 of the present invention further increased the final viscosity and recovery value of millet gelatinization.

[0046] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A millet foliar spray, characterized in that: Calculated by weight, the composition comprises 15-18 parts of methyl jasmonate, 6-9 parts of salicylic acid and 190-210 parts of a surfactant.

2. The foliar spray according to claim 1, characterized in that: Calculated by mass, it includes 16 parts of methyl jasmonate, 8 parts of salicylic acid and 200 parts of surfactant.

3. The millet foliar spray according to claim 1 or 2, characterized in that: The surfactant is polyethylene glycol octylphenyl ether (Triton X-100).

4. The method for preparing the millet foliar spray according to any one of claims 1 to 3, characterized in that: Weigh the raw materials methyl ester, salicylic acid and surfactant in proportion and mix them evenly; dilute with water before use.

5. The preparation method according to claim 4, characterized in that: The dilution factor is 2×10 5 times.

6. Use of the millet foliar spray according to any one of claims 1 to 3 in increasing the lutein content, yellow pigment content, total flavonoid content and gelatinization characteristics of millet.

7. The use according to claim 6, characterized in that: The millet foliar spray is applied on the leaves after the grains are formed during the millet grain filling stage.

8. The use according to claim 7, characterized in that: The spraying frequency is 3 to 4 times.