Suspended seed coating and application thereof in prevention and treatment of potato cyst nematode disease and late blight

Abstract

The invention discloses a suspended seed coating and application thereof in prevention and treatment of potato cyst nematode disease and late blight, and belongs to the technical field of pesticides. The invention discloses a suspended seed coating. The effective components of the suspended seed coating are fluopyram, metalaxyl-M and fludioxonil. The suspended seed coating agent disclosed by the invention is good in film-forming property and uniform in coating, and does not influence the emergence rate of potato seeds. Compared with single agents, the suspended seed coating disclosed by the invention not only can prevent and treat the potato cyst nematode disease, but also has a certain prevention and treatment effect on potato late blight, and has a synergistic effect. The suspended seed coating agent disclosed by the invention is time-saving and labor-saving when being used for treating seed potatoes, can delay the drug resistance of diseases, and has a very good application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and more particularly to a suspended seed coating agent and application thereof in preventing and controlling potato cyst nematode disease and late blight. Background Art

[0002] Potato late blight (Phytophthora infestans) is a crop disease caused by oomycetes, which poses a great threat to my country's potato industry. This disease mainly harms the stems, leaves and tubers of potatoes, and can cause a yield loss of 10-20%; in the year of severe occurrence, this figure may be as high as 50-70%, resulting in a significant reduction in crop yields, and even a total crop failure in severe cases. Potato cyst nematode disease is another important disease that harms potato cultivation, mainly including potato golden wireworm (Globodera rostochiensis) and potato white wireworm (G. pallida). This disease was first discovered in Europe and is now common in countries such as the United Kingdom, the Netherlands, Germany, France, and Belgium. According to statistics, about 64% of potato fields in the European Union are affected by potato cyst nematode disease, which generally causes a 20% reduction in potato yields. In severe cases, it causes a yield loss of 80-90%, or even a total crop failure.

[0003] Fluopyram is a broad-spectrum fungicide belonging to the pyrazole amide class of compounds. It was later found to have a good control effect on plant nematode diseases. It can inhibit the energy metabolism of fungal cells by inhibiting complex II (succinate dehydrogenase) in the mitochondrial respiratory chain, thereby achieving the effect of killing fungi / nematodes. Fluopyram has excellent systemic absorption and conductivity, can be quickly absorbed by plants and transmitted in the body, and effectively prevents and controls diseases on a variety of crops.

[0004] Metalaxyl-M is a broad-spectrum, highly effective fungicide belonging to the phenylamide class of compounds. It is mainly used to control a variety of crop diseases caused by Oomycete pathogens, especially downy mildew and late blight. Metalaxyl-M achieves the purpose of sterilization by inhibiting RNA polymerase in pathogen cells and preventing nucleic acid synthesis.

[0005] Fludioxonil is a broad-spectrum, highly effective fungicide belonging to the benzimidazole class of compounds. It is mainly used to control a variety of crop diseases caused by ascomycetes, basidiomycetes and deuteromycetes, especially for diseases during storage and transportation. Fludioxonil achieves the purpose of sterilization by interfering with the chitin synthesis of the pathogen cell wall and preventing its normal development.

[0006] Seed dressing agent is an ideal preparation for preventing and controlling root diseases of crops. Treating potato seed potatoes with seed dressing agent is a commonly used agronomic measure. It can effectively kill pathogens attached to the surface of seed potatoes and reduce the risk of soil-borne diseases, especially some difficult-to-cure diseases such as nematode disease, late blight, ring rot, mole disease, wilt, etc.

[0007] Therefore, providing a suspended seed coating agent and its application in preventing and controlling potato cyst nematode disease and late blight is a problem that those skilled in the art urgently need to solve. Summary of the invention

[0008] In view of this, the present invention provides a suspension seed coating agent and its application in preventing and controlling potato cyst nematode disease and late blight. The suspension seed coating agent is a compound seed coating agent containing fluopyram, metalaxyl-M and fludioxonil, which can prevent potato cyst nematode disease and potato late blight.

[0009] In order to achieve the above object, the present invention adopts the following technical solution:

[0010] A suspended seed coating agent contains the following components by mass percentage: 5-40% of fluopyram, 1.8-3.5% of metalaxyl-M, 1.2-2.5% of fludioxonil, 7.6-33% of adjuvant, and water supplemented to 100%.

[0011] Furthermore, the auxiliary agent contains the following components: 2-5% dispersant, 1-6% wetting agent, 0.5-3% film former, 0.1-3% stabilizer, 1-5% antifreeze agent, 1-5% defoaming agent, 1-3% preservative, and 1-3% colorant.

[0012] Further, the dispersant is a polyoxyethylene polyoxypropylene ether block copolymer, nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, octylphenol polyoxyethylene ether, calcium lignin sulfonate, polycarboxylate, sodium lauryl alcohol polyoxyethylene ether sulfate, triphenyl phenol polyoxyethylene ether or sodium lignin sulfonate;

[0013] The wetting agent is sodium dodecylbenzene sulfonate, sodium dodecyl sulfate or pull open powder;

[0014] The film-forming agent is gum arabic, pectin, methyl cellulose, ethyl cellulose, polyvinyl alcohol, polyethylene glycol, animal glue, hydroxypropyl cellulose, sodium alginate or polyvinyl pyrrolidone;

[0015] The stabilizer is xanthan gum, diatomaceous earth, sodium carboxymethyl cellulose, magnesium aluminum silicate, bentonite, cellulose ether and its derivatives or white carbon black;

[0016] The antifreeze agent is ethylene glycol, propylene glycol or glycerol;

[0017] The defoamer is an organosilicon defoamer;

[0018] The preservative is potassium sorbate, industrial grade formaldehyde or sodium benzoate;

[0019] The colorant is acid scarlet, Congo red or alkaline rose essence.

[0020] Furthermore, the above-mentioned suspension seed coating agent is used in preventing and controlling potato cyst nematode disease and late blight.

[0021] It can be known from the above technical scheme that compared with the prior art, the present invention discloses a suspension seed coating agent and its application in preventing and controlling potato cyst nematode disease and late blight. Compared with the simple fungicide metalaxyl-M and fludioxonil, or fluopyram single agent, the suspension seed coating agent of the present invention can effectively reduce the dosage, reduce the cost, and expand the scope of disease prevention and control; fluopyram is currently widely used as a nematicide with outstanding prevention effect. Since the agent itself is also a fungicide, mixing it with metalaxyl-M and fludioxonil is beneficial to enhance the fungicide effect, while reducing the risk of drug resistance of potato late blight, achieving the effect of treating potato nematode disease and potato late blight at the same time. Moreover, the seed coating agent is used to treat the seed potatoes before sowing potatoes, and the harm of potato cyst nematode and late blight can be effectively reduced by applying the drug once, which is an ideal product for potato disease prevention and control. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] Weigh the following raw materials by mass ratio: 5% fluopyram, 3.5% metalaxyl-M, 2.5% fludioxonil, 5% triphenol polyoxyethylene ether, 2% sodium lauryl sulfate, 3% polyvinyl pyrrolidone, 0.15% sodium carboxymethyl cellulose, 2% glycerol, 1% organosilicon defoamer, 1.5% potassium sorbate, 1% acid red, and make up to 100% with water.

[0025] Preparation method of suspended seed coating agent: according to the formula requirements, accurately weigh each ingredient, and add the above ingredients and deionized water into a beaker in turn. Start the high-speed shear mixer and continue processing for 10 to 20 minutes to ensure that all ingredients are fully mixed. Then, use a sand mill to finely grind until more than 95% of the particles reach a particle size of 1 to 5 microns, and the average particle size is kept within 3 microns. The final red suspension is the suspended seed coating agent.

[0026] Example 2

[0027] The raw materials were weighed according to the mass ratio: 10% fluopyram, 3% metalaxyl-M, 2% fludioxonil, 3% nonylphenol polyoxyethylene ether, 1% lakai powder, 2% polyvinyl alcohol, 0.1% cellulose ether, 5% glycerol, 3% organosilicon defoamer, 1.5% potassium sorbate, 2% alkaline rose essence, and water was added to 100%. The suspension seed coating agent was prepared using the above raw materials according to the preparation method of Example 1.

[0028] Example 3

[0029] Weigh the raw materials by mass ratio: 15% fluopyram, 2.5% metalaxyl-M, 1.5% fludioxonil, 2% fatty alcohol polyoxyethylene ether, 6% sodium dodecylbenzene sulfonate, 0.5% hydroxypropyl cellulose, 3% magnesium aluminum silicate, 1% propylene glycol, 5% organosilicon defoamer, 1.5% potassium sorbate, 2% alkaline rose essence, and make up to 100% with water. Prepare a suspension seed coating agent with the above raw materials according to the preparation method of Example 1.

[0030] Example 4

[0031] Weigh the raw materials by mass ratio: 20% fluopyram, 1.8% metalaxyl-M, 1.2% fludioxonil, 3% calcium lignin sulfonate, 2% sodium lauryl sulfate, 2% sodium alginate, 0.15% diatomaceous earth, 2% ethylene glycol, 1% organosilicon defoamer, 1% sodium benzoate, 2% acid red, and make up to 100% with water. Prepare a suspension seed coating agent with the above raw materials according to the preparation method of Example 1.

[0032] Example 5

[0033] Weigh the raw materials by mass ratio: 40% fluopyram, 1.8% metalaxyl-M, 1.2% fludioxonil, 4% octylphenol polyoxyethylene ether, 4% sodium lauryl sulfate, 2% gum arabic, 0.15% xanthan gum, 2% glycerol, 3% organosilicon defoamer, 3% industrial formaldehyde, 3% Congo red, and make up to 100% with water. Prepare a suspension seed coating agent with the above raw materials according to the preparation method of Example 1.

[0034] Test case: Field efficacy test

[0035] Test method:

[0036] The suspended seed coating agent prepared in Examples 1 to 5 was used to treat potato seed potatoes before sowing, and the amount of the suspended seed coating agent was 100 ml / 100 kg of seed potatoes. The suspended seed coating agent was diluted with water and sprayed on the seed potatoes and stirred to make the liquid medicine evenly distributed on the surface of the seed potatoes. After the seed potatoes were coated, they were dried in a ventilated place. The suspended seed coating agent had good film-forming properties and the coating was uniform.

[0037] The treatment group planted coated seed potatoes, while the control group planted uncoated seed potatoes. The test site was located in a field in the mountainous area of ​​southwest my country where potato cyst nematode disease and late blight have been reported. The potato variety was Qingshu No. 9. Each treatment was repeated three times, and each plot was 45m2. 2 . 41.7% Fluopyram suspension concentrate (Bayer AG) and metalaxyl-fludioxonil suspension seed coating agent (total active ingredient 62.5 g / L, including 37.5 g / L metalaxyl and 25 g / L fludioxonil; Guangdong Zhenge Biotechnology Co., Ltd.) were used as comparison agents, and the dosage of the suspension seed coating agent was 100 ml / 100 kg seed potatoes.

[0038] 1) Seedling emergence rate survey:

[0039] After potato sowing, investigate the emergence rate at the bud stage. The calculation formula is as follows:

[0040] Seedling rate (%) = number of seedlings / total number of seeds sown x 100

[0041] 2) Prevention effectiveness investigation:

[0042] (1) Investigation on the effectiveness of late blight prevention

[0043] During the peak period of potato late blight, the leaf disease situation was investigated, and the disease index and control effect were calculated. The grading standard and calculation formula are as follows:

[0044] Late blight classification standards (GB / T 17980.34 National Standard of the People's Republic of China; Field Efficacy Test Guidelines (I) for the control of potato late blight by fungicides):

[0045] According to the leaf spot area, the disease is divided into 0 to 9 levels, as follows:

[0046] Level 0: no lesions;

[0047] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0048] Level 3: The lesion area accounts for 6-10% of the entire leaf area;

[0049] Level 5: The lesion area accounts for 11-20% of the entire leaf area;

[0050] Level 7: The lesion area accounts for 21-50% of the entire leaf area;

[0051] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0052]

[0053] (2) Investigation on the effectiveness of prevention against potato cyst nematode disease

[0054] The number of cysts per unit volume of soil in the test plots was counted before potato sowing, and the number of cysts per unit volume of soil and plant roots in each plot was counted after potato harvest. The cyst reduction rate and control effect were calculated using the following formula:

[0055]

[0056] The effects of the suspended seed coating agent on emergence rate and yield, as well as the control efficacy against potato cyst nematode disease and late blight are shown in Table 1.

[0057] Table 1 The efficacy of suspension seed coating agent against potato cyst nematode and late blight

[0058]

[0059] As can be seen from Table 1, after potatoes were treated with the suspension seed coating agents of Examples 1 to 5, the emergence rate of potato seed potatoes in each plot was not significantly different from that of the control plot, indicating that the suspension seed coating agent was safe for potato emergence. The suspension seed coating agents of Examples 1 to 5 were significantly better than the metalaxyl-fludioxonil suspension seed coating agent in preventing potato cyst nematode disease, and significantly better than the fluopyram suspension agent in preventing potato spore late blight; at the same time, the prevention effect of Examples 4 and 5 on potato cyst nematode disease was close to or even better than the prevention effect of fluopyram single agent, but the fluopyram content in Example 4 was only half of that of the single agent, which had a higher prevention and control efficiency. In addition, compared with the metalaxyl-fludioxonil suspension seed coating agent, Examples 2, 4, and 5 had similar prevention effects on potato late blight. A comprehensive comparison of the yield-increasing effects showed that the yield-increasing effects of Examples 4 and 5 were the most obvious. The above results show that the suspension seed coating agent of the present invention has a significant disease prevention and yield-increasing effect on potatoes, among which the combination of myclobutanil accounting for 20% of the total mass of the raw materials, metalaxyl accounting for 1.8% of the total mass of the raw materials, and fludioxonil accounting for 1.2% of the total mass of the raw materials (Example 4) has the highest efficiency.

[0060] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A suspension seed coating agent, characterized in that: The invention contains the following components by mass percentage: 5-40% of fluopyram, 1.8-3.5% of metalaxyl-M, 1.2-2.5% of fludioxonil, 7.6-33% of auxiliary agent, and water is added to 100%.

2. A suspension seed coating agent according to claim 1, characterized in that: The auxiliary agent contains the following components: 2-5% dispersant, 1-6% wetting agent, 0.5-3% film former, 0.1-3% stabilizer, 1-5% antifreeze agent, 1-5% defoamer, 1-3% preservative and 1-3% colorant.

3. A suspension seed coating agent according to claim 2, characterized in that: The dispersant is a polyoxyethylene polyoxypropylene ether block copolymer, nonylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, octylphenol polyoxyethylene ether, calcium lignin sulfonate, polycarboxylate, sodium lauryl alcohol polyoxyethylene ether sulfate, triphenyl phenol polyoxyethylene ether or sodium lignin sulfonate; The wetting agent is sodium dodecylbenzene sulfonate, sodium dodecyl sulfate or pull open powder; The film-forming agent is gum arabic, pectin, methyl cellulose, ethyl cellulose, polyvinyl alcohol, polyethylene glycol, animal glue, hydroxypropyl cellulose, sodium alginate or polyvinyl pyrrolidone; The stabilizer is xanthan gum, diatomaceous earth, sodium carboxymethyl cellulose, magnesium aluminum silicate, bentonite, cellulose ether and its derivatives or white carbon black; The antifreeze agent is ethylene glycol, propylene glycol or glycerol; The defoamer is an organosilicon defoamer; The preservative is potassium sorbate, industrial grade formaldehyde or sodium benzoate; The colorant is acid scarlet, Congo red or alkaline rose essence.

4. Use of a suspension seed coating agent according to any one of claims 1 to 3 in preventing and controlling potato cyst nematode disease and late blight.

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