A pesticide composition, its preparation method and application

Abstract

The application provides a pesticide composition, a preparation method and application thereof, and belongs to the technical field of pesticides.The active ingredient of the pesticide composition is composed of acetylamino-avermectin B2a and fluopyram, and the weight ratio of the acetylamino-avermectin B2a and the fluopyram is 30:1-1:30;the preparation method is that acetylamino-avermectin B2a, fluopyram, wetting dispersant, thickening agent, antifreezing agent, defoaming agent and water are mixed according to a predetermined proportion, wet sand grinding is carried out, and water suspension concentrate with a particle size D90 of less than or equal to 5 microns is obtained;the pesticide composition is used for preventing and treating root nematodes of vegetables, cereals and flowers, and the application mode is hole application, trench application or root irrigation.The pesticide composition is high in efficiency, low in toxicity, friendly to the environment, and has a high social value.

Description

Technical Field

[0001] This invention relates to a pesticide composition, and more particularly to a nematicide pesticide composition containing acetaminophen B2a and its application, belonging to the field of pesticide technology. Background Technology

[0002] Avermectin is produced by *Sterptomyces avermitills* and is a type of streptomycin belonging to the grey streptomycin family. Avermectin is a mixture of fermentation components, comprising eight parts: A1a, A1b, A2a, A2b, B1a, B1b, B2a, and B2b. Currently, avermectin is primarily used as an agricultural insecticide and can also be used as a veterinary anthelmintic. Its mechanism of action is mainly through acting on GABA A receptors at the synapses of insect neurons or neuromuscular synapses, interfering with the transmission of information at nerve endings within the insect. Specifically, it stimulates nerve endings to release the neurotransmission inhibitor gamma-aminobutyric acid (GABA), causing GABA-gated chloride ion channels to prolong their opening. This activation of chloride ion channels leads to a massive influx of chloride ions, causing hyperpotentialization of the nerve membrane, resulting in an inhibitory state of the nerve membrane. This blocks the connection between nerve endings and muscles, causing paralysis, loss of appetite, and death in the insect. Acetaminophen B2a is a derivative of avermectin. Recent studies have found that acetaminophen B2a has a certain control effect on nematodes in plant roots. Whether it has a synergistic effect with other nematicides still needs further research.

[0003] Fluopyram, discovered by Bayer in 2002 and marketed in 2012, is a novel pesticide belonging to the succinate dehydrogenase inhibitor class of fungicides and nematicides. It is also used as a seed treatment agent and a preservative for agricultural products. Fluopyram's mechanism of action involves inhibiting the mitochondrial electron transport chain and succinate dehydrogenase activity in the tricarboxylic acid cycle of pathogens, interfering with respiration, hindering energy production, and thus killing the pathogens. Fluopyram is not only a broad-spectrum, systemic fungicide but also the first nematicide to inhibit Complex II, representing a new type of nematicide with a promising market prospect. Fluopyram effectively prevents nematode infestation and is the first SDHI-class nematicide, as well as the first nematicide with low toxicity to users and the environment. Although fluopyram has excellent properties and outstanding characteristics, the inherent resistance risk of SDHI fungicides is classified as medium to high by FRAC. Summary of the Invention

[0004] To address the above problems, the present invention provides a pesticide composition, its preparation method, and its application to improve nematicidal activity.

[0005] To achieve the above objectives, the technical solution of the present invention is as follows: a pesticide composition, wherein the active ingredients of the pesticide composition are composed of acetaminophen B2a and fluopyram, and the weight ratio of acetaminophen B2a to fluopyram is 30:1 to 1:30.

[0006] Furthermore, the weight ratio of acetaminophen B2a to fluopyram is 1:1.

[0007] Furthermore, the pesticide composition is an aqueous suspension, which includes acetaminophen B2a, fluopyram, wetting and dispersing agents, thickeners, antifreeze agents, and defoamers.

[0008] Furthermore, the aqueous suspension comprises, by weight percentage, 1-30% acetaminophen B2a, 1-30% fluopyram, 1-10% wetting and dispersing agent, 0.5-3% thickener, 2-6% antifreeze agent, 0.1-1% defoamer, and water to make up 100%.

[0009] Furthermore, the wetting and dispersing agent is one to three of the following: tristyrene polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate, and sodium dioctyl succinate sulfonate.

[0010] Furthermore, the thickener is one to three of the following: magnesium aluminum silicate, silica, xanthan gum, sodium carboxymethyl cellulose, gum arabic, and polyethylene glycol.

[0011] Furthermore, the antifreeze is one or two of the following: ethylene glycol, propylene glycol, glycerin, and urea.

[0012] Furthermore, the defoamer is an organosilicone defoamer.

[0013] The above-mentioned pesticide composition is prepared by mixing acetaminophen B2a, fluopyram, wetting and dispersing agent, thickener, antifreeze agent, defoamer and water in a predetermined ratio, and then grinding them into an aqueous suspension with a particle size D90≤5μm by wet sand milling.

[0014] The above-mentioned pesticide composition is used to control root nematodes in vegetables, cereals and flowers, and can be applied by hole application, trench application or root irrigation.

[0015] The beneficial effects of the pesticide composition, its preparation method, and its application according to the present invention are as follows:

[0016] The pesticide composition of this invention is highly efficient, low in toxicity, and environmentally friendly. The combination of the two agents has a significant synergistic effect, which improves the nematicidal activity and can also effectively reduce the amount of pesticides used, in line with the national dual reduction measures, and has high social value.

[0017] This invention utilizes a combination of acetaminophen B2a and fluopyram, which exhibits a good synergistic effect and is significantly more effective than single agents. Furthermore, the active ingredients in the nematicidal composition have different mechanisms of action, reducing the development of resistance under single-agent action and delaying the development of resistance in nematodes to single agents. Attached Figure Description

[0018] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0019] Figure 1 The image shows the results of root-knot nematode infestation on cucumber roots after treatment with different agents according to the present invention. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0021] A pesticide composition wherein the active ingredients of the pesticide composition consist of acetaminophen B2a and fluopyram, wherein the weight ratio of acetaminophen B2a to fluopyram is 30:1 to 1:30. Preferably, the weight ratio of acetaminophen B2a to fluopyram is 1:1.

[0022] Preferably, the pesticide composition is an aqueous suspension, which includes acetaminophen B2a, fluopyram, a wetting and dispersing agent, a thickener, an antifreeze agent, and an antifoaming agent. More preferably, the aqueous suspension comprises, by weight percentage, 1-30% acetaminophen B2a, 1-30% fluopyram, 1-10% wetting and dispersing agent, 0.5-3% thickener, 2-6% antifreeze agent, 0.1-1% antifoaming agent, and water to make up 100%.

[0023] Preferably, the wetting and dispersing agent is one to three of the following: tristyrene polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate, and sodium dioctyl succinate sulfonate; the thickener is one to three of the following: magnesium aluminum silicate, silica, xanthan gum, sodium carboxymethyl cellulose, gum arabic, and polyethylene glycol; the antifreeze agent is one to two of the following: ethylene glycol, propylene glycol, glycerin, and urea; and the defoamer is an organosilicon defoamer.

[0024] The above-mentioned pesticide composition is prepared by mixing acetaminophen B2a, fluopyram, wetting and dispersing agent, thickener, antifreeze agent, defoamer and water in a predetermined ratio, and then grinding them into an aqueous suspension with a particle size D90≤5μm by wet sand milling.

[0025] The above-mentioned pesticide composition is used to control root nematodes in vegetables, cereals and flowers, and can be applied by hole application, trench application or root irrigation.

[0026] Example 1: 5% acetaminophen + 10% fluopyram aqueous suspension

[0027] 5% acetaminophen B2a, 10% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 1% naphthalene sulfonate, 0.5% EO / PO block polyether, 0.25% xanthan gum, 0.4% silicone defoamer, 5% propylene glycol, water to 100%, wet-milled to a particle size D90≤5um water suspension.

[0028] Example 2: 5% acetaminophen B2a + 5% fluopyram aqueous suspension

[0029] 5% acetaminophen B2a, 5% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 1% polycarboxylate, 1% fatty alcohol polyoxyethylene ether, 1.5% magnesium aluminum silicate, 0.25% xanthan gum, 0.4% silicone defoamer, 5% propylene glycol, water to 100%, wet-milled to a particle size D90≤5um water suspension.

[0030] Example 3: 10% acetaminophen B2a + 5% fluopyram aqueous suspension

[0031] 10% acetaminophen B2a, 5% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 0.5% lignin sulfonate, 1% fatty alcohol polyoxyethylene ether, 1% silica, 0.22% xanthan gum, 0.4% silicone defoamer, 5% ethylene glycol, water to 100%, wet-milled to a particle size D90≤5um water suspension.

[0032] Example 4: 15% acetaminophen B2a + 5% fluopyram aqueous suspension

[0033] 15% acetaminophen B2a, 5% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 1% lignin sulfonate, 1% sodium dioctyl succinate sulfonate, 1.5% sodium carboxymethyl cellulose, 0.2% xanthan gum, 0.4% silicone defoamer, 2% urea, 3% glycerin, and water to 100%. The mixture is wet-milled to a particle size D90≤5µm and then used as an aqueous suspension.

[0034] Example 5: 5% acetaminophen B2a + 15% fluopyram aqueous suspension

[0035] 5% acetaminophen B2a, 15% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 0.5% lignin sulfonate, 1% polycarboxylate, 1.5% sodium carboxymethyl cellulose, 0.15% xanthan gum, 0.4% silicone defoamer, 2% ethylene glycol, 3% urea, and water to 100%. The mixture is wet-milled to a particle size D90≤5µm and then used as an aqueous suspension.

[0036] Example 6: 1% acetaminophen B2a + 30% fluopyram aqueous suspension

[0037] 1% acetaminophen B2a, 30% fluopyram, 3% tristyrene polyoxyethylene ether phosphate, 1% lignin sulfonate, 1.5% polycarboxylate, 1% sodium carboxymethyl cellulose, 0.1% xanthan gum, 0.4% silicone defoamer, 2% propylene glycol, 1% urea, and water to 100%. The mixture is wet-milled to a particle size D90≤5µm and then used as an aqueous suspension.

[0038] Example 7: 30% acetaminophen B2a + 1% fluopyram aqueous suspension

[0039] 30% acetaminophen B2a, 1% fluopyram, 3% EO / PO block polyether, 1.5% lignin sulfonate, 2% polycarboxylate, 1.5% sodium carboxymethyl cellulose, 0.1% xanthan gum, 0.8% silicone defoamer, 2% propylene glycol, 2% urea, water to 100%, wet-milled to a particle size D90≤5um, water suspension.

[0040] Application test case

[0041] The pesticide compositions prepared in Examples 1 and 2 were applied to control cucumber root-knot nematode disease, as follows:

[0042] One week after cucumber seedling transplanting, root irrigation was performed using each treatment (as shown in Table 1 below). After the cucumber harvest, a five-point sampling method was used in each treatment area, with four plants selected at each point. The number of diseased plants at each level for each treatment was counted, and the disease index and control effect of different treatments were calculated. The results are shown in Table 1 and... Figure 1 As shown.

[0043]

[0044] Diseased plant grading standards:

[0045] No root knots were observed at the level 0 root.

[0046] First-order fibrous roots with few root knots, no main root.

[0047] Secondary fibrous roots show relatively large root knots, while the main root is absent.

[0048] Level 3: 50% of roots have root knots, and some taproots have root knots.

[0049] The fourth-order taproot has many root knots.

[0050] All 5-level taproots have root knots

[0051] CK disease index: Disease index of the water control group

[0052] Table 1: Application Examples of Nematicide Composition Aqueous Suspension Formulations and Their Efficacy Against Cucumber Root-Knot Nematodes

[0053]

[0054] Synergistic effect verification test case

[0055] Toxicity determination of acetaminophen B2a combined with fluopyram against southern root-knot nematodes:

[0056] The experiment was conducted in accordance with NY / T 1833.1-2009 "Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides" Part 1: Inhibition of Plant Pathogenic Nematodes Test - Immersion Method.

[0057] Wash fresh plant roots infected with root-knot nematodes, cut them into 1cm long segments, put them in a tissue homogenizer with 3 times the amount of water and homogenize for 30 seconds. Transfer the homogenate to a combination of 200-mesh (75μm) and 500-mesh (26μm) standard sieves, rinse the plant tissue in the sieves with tap water, collect the nematode eggs in the 500-mesh standard sieve, surface sterilize with 0.5% NaClO solution for 3 minutes, rinse 5 times with sterile water, and then place them in a self-made Behman funnel and incubate in the dark at 25℃. Collect southern root-knot nematodes every 24 hours for later use.

[0058] According to the experimental design, the test agent was serially diluted with 0.1% Triton aqueous solution, with 0.1% Triton aqueous solution as a control. The root-knot nematodes collected within 24 hours were prepared into a suspension with water (about 200 nematodes / mL). 0.5 mL of the drug solution and 0.5 mL of the nematode suspension were transferred into a 24-well plate using a pipette. The experiment was repeated 3 times. After mixing with a fine glass rod, the plates were placed in a 25°C incubator in the dark. After 72 hours, the total number of nematodes and the number of dead nematodes were checked, and the mortality rate and corrected mortality rate were calculated.

[0059] The mortality of nematodes was determined by needle puncture under a stereomicroscope. The number of dead nematodes was counted, and the mortality rate and corrected mortality rate were calculated using the following formulas:

[0060] Mortality rate (%) = Number of dead nematodes / Number of treated nematodes × 100%

[0061] Corrected mortality rate (%) = (treatment mortality rate - control mortality rate) / (100 - control mortality rate) × 100%

[0062] If the control mortality rate is less than 5%, no correction is needed; if the control mortality rate is between 5% and 15%, the corrected mortality rate should be calculated according to the formula; if the control mortality rate is greater than 15%, the trial needs to be repeated.

[0063] A linear regression equation was established with the concentration used in the test as the x-axis and the survival probability value corresponding to the mortality rate (obtained from a biostatistical probability conversion table) as the y-axis, resulting in the toxicity regression equation. Substituting the mortality rate of 50% (i.e., a survival probability of 5) into the toxicity regression equation, the LC50 of the drug was obtained. 50 value.

[0064] The combined effect of the insecticidal combination of acetaminophen B2a and fluopyram on nematodes was evaluated using the co-toxicity coefficient method (fluopyram as the standard agent).

[0065] Actual Toxicity Index (ATI) = (LC50 of Standard Reagent) 50 / LC of the test reagent 50 )×100

[0066] Theoretical Toxicity Index (TTI) = Toxicity Index of Agent A × Percentage of A in the Mixture + Toxicity Index of Agent B × Percentage of B in the Mixture

[0067] Cotoxicity coefficient (CTC) = (ATI / TTI) × 100

[0068] A co-toxicity coefficient (CTC) greater than 120 indicates a synergistic effect; a co-toxicity coefficient between 80 and 120 indicates an additive effect; and a co-toxicity coefficient less than 80 indicates an antagonistic effect.

[0069] When the observed control effect exceeds the expected control effect, it indicates that there is an synergistic effect.

[0070] The results of the toxicity test are shown in the table below:

[0071] Table 2: Results of indoor toxicity assays of individual active ingredients against Southern Root-knot Nematode

[0072]

[0073] Table 3: Toxicity test results of the combination of acetaminophen B2a and fluopyram.

[0074]

[0075]

[0076] The experimental results show that when acetaminophen B2a and fluopyram are combined to control southern root-knot nematodes, the ratio between 1:30 and 30:1 has a synergistic effect.

[0077] Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A pesticidal composition, characterized by, The active ingredient of the pesticide composition consists of acrinathin and fluopyram, and the weight ratio of acrinathin to fluopyram is 30:1-1:

30.

2. The pesticide composition according to claim 1, wherein The weight ratio of acrinathin to fluopyram is 1:

1.

3. The pesticide composition according to claim 1 or 2, characterized in that, The pesticide composition is a water suspension agent, which comprises acrinathin, fluopyram, wet dispersant, thickening agent, antifreeze and defoaming agent.

4. The pesticidal composition according to claim 3, wherein The water suspension agent comprises acrinathin 1-30%, fluopyram 1-30%, wet dispersant 1-10%, thickening agent 0.5-3%, antifreeze 2-6%, defoaming agent 0.1-1% by weight, and water makes up 100%.

5. The pesticidal composition according to claim 3, wherein The wet dispersant is 1-3 kinds of triphenyl ethenyl polyoxyethylene ether phosphate, polycarboxylate, fatty alcohol polyoxyethylene ether, EO / PO block polyether, lignin sulfonate, naphthalene sulfonate and succinic acid dioctyl ester sodium sulfonate.

6. The pesticidal composition according to claim 3, wherein The thickening agent is 1-3 kinds of magnesium aluminum silicate, white carbon black, xanthan gum, sodium carboxymethyl cellulose, gum arabic and polyethylene glycol.

7. The pesticide composition according to claim 3, wherein The antifreeze is 1-2 kinds of ethylene glycol, propylene glycol, glycerol and urea.

8. The pesticide composition according to claim 3, wherein The defoaming agent is a silicone defoaming agent.

9. A process for the preparation of the pesticide composition according to claims 1-8, characterized in that, Mix acrinathin, fluopyram, wet dispersant, thickening agent, antifreeze, defoaming agent and water in a predetermined proportion, wet sand mill and grind to a water suspension agent with particle size D90≤5μm.

10. Use of a pesticide composition according to claims 1 to 8 or of a pesticide composition prepared according to claim 9, characterized in that, It is used for controlling root nematodes of vegetables, cereals and flowers, and the application mode is hole application, trench application or root irrigation.

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