Anti-fatigue high-efficiency sugar-reducing sports beverage based on biotechnology and preparation method of anti-fatigue high-efficiency sugar-reducing sports beverage

Abstract

The invention relates to the technical field of anti-fatigue beverages, in particular to an anti-fatigue high-efficiency sugar-reducing sports beverage based on biotechnology and a preparation method thereof, and the anti-fatigue high-efficiency sugar-reducing sports beverage comprises the following components: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of a hawthorn extract, 6-12 parts by weight of a green tea extract and the like. According to the invention, methyl glucoside and salidroside are added for etherification reaction, the generated etherification product is more stable when the environment changes, and effective components are not easy to decompose; meanwhile, the water solubility of methyl glucoside is improved to promote the absorption of salidroside, and the anti-fatigue effect is indirectly enhanced, so that the fatigue is relieved more quickly.

Description

Technical Field

[0001] The invention relates to the technical field of anti-fatigue beverages, in particular to an anti-fatigue, high-efficiency, sugar-reduced sports beverage based on biotechnology and a preparation method thereof. Background Art

[0002] Currently, most sports drinks on the market are based on artificial synthetic ingredients and high-sugar formulas. Although traditional high-sugar sports drinks can quickly replenish energy, there is a risk of blood sugar fluctuations for diabetic patients, people who are concerned about their health, and consumers who pursue a low-sugar diet. Long-term drinking may cause health problems such as obesity and cardiovascular disease, limiting its consumer group. In addition, some artificial synthetic ingredients may pose potential health risks, and consumers are increasingly demanding more natural and healthier sports drinks.

[0003] Plant extracts are rich in a variety of bioactive ingredients, such as flavonoids, polysaccharides, saponins, etc., which have multiple functions such as antioxidant, anti-fatigue, and immune regulation; however, the active ingredients in plant extracts have complex chemical structures. During processing and storage, their effective ingredients are easily degraded by factors such as light, temperature, and oxygen, thereby reducing their efficacy. In view of this, we propose an anti-fatigue, high-efficiency, and sugar-reduced sports drink based on biotechnology and its preparation method. Summary of the invention

[0004] The purpose of the present invention is to provide an anti-fatigue, high-efficiency, sugar-reduced sports drink based on biotechnology and a preparation method thereof, so as to solve the problem in the above-mentioned background technology that the active ingredients in the plant extracts have complex chemical structures, and during processing and storage, the effective ingredients are easily affected by factors such as light, temperature, oxygen, etc. and degraded, thereby reducing the efficacy.

[0005] To achieve the above object, the present invention provides an anti-fatigue and high-efficiency sugar-reducing sports drink based on biotechnology, comprising the following components: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of hawthorn extract, 6-12 parts by weight of green tea extract, 3-8 parts by weight of guarana extract, 8-15 parts by weight of wolfberry extract, 20-30 parts by weight of erythritol, 1-3 parts by weight of sodium chloride, 0.5-2 parts by weight of potassium chloride, 2-5 parts by weight of acidulant, 1-3 parts by weight of gamma-aminobutyric acid, 1-3 parts by weight of L-ornithine, 0.5-2 parts by weight of coenzyme Q10, 0.5-2 parts by weight of choline, and 0.5-2 parts by weight of sodium carboxymethyl cellulose; The etherified salidroside is prepared by etherifying salidroside with methyl glucoside and adding polyethylene glycol-phosphorylcholine.

[0006] Preferably, the preparation method of the etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 10-20 parts by weight of salidroside, 15-30 parts by weight of methyl glucoside, 3-8 parts by weight of polyethylene glycol-phosphorylcholine, and 5-12 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, dissolve salidroside and methyl glucoside in deionized water, stir at 100-200 rpm for 30-60 min, and add potassium carbonate solution to adjust the pH value of the solution; slowly add polyethylene glycol-phosphorylcholine, and place the reaction system in a constant temperature water bath at 40-60°C, and stir at 300-400 rpm for 10-24 h; Under nitrogen protection and potassium carbonate alkaline conditions, the 7-hydroxyl group of salidroside is first deprotonated to form a nucleophilic alkoxy anion; this anion attacks the 2-hydroxyl carbon (β-D configuration) of methyl glucoside, initiating a nucleophilic substitution reaction; the 2-hydroxyl group of methyl glucoside is activated under the action of alkali, and its leaving group (-OH) combines with the alkoxy anion to form an ether bond.

[0007] Some groups in the salidroside molecule (such as hydroxyl groups) have certain reactivity and are easy to react with external substances or change themselves under specific conditions; through the etherification reaction with methyl glucoside, these active groups are converted into relatively stable ether bond structures. The ether bond has a high bond energy and is not easy to break under general environmental conditions, thereby improving the stability of the entire molecule; the solubility of salidroside itself may be limited, while methyl glucoside has multiple hydroxyl groups and is a highly hydrophilic substance; after the etherification reaction, the structural fragments of methyl glucoside are introduced into the product molecule, which increases the interaction sites between the molecule and the water molecule, making the salidroside more stable. More hydrogen bonds and other forces can be formed between salidroside and water molecules, thereby increasing the solubility of the product in water; the etherification reaction changes the molecular structure of salidroside. On the one hand, it makes the original salidroside bind to the target more tightly and specifically, thereby enhancing the original biological activity; on the other hand, the newly introduced methyl glucoside structure will give the product the ability to interact with other targets, thereby generating new biological activity; after the etherification reaction, some groups in salidroside are modified, and the overall properties of the molecule are changed, which reduces the interaction with sensitive components in biological tissues when it comes into contact with skin, mucous membranes, etc., and reduces the possibility of causing irritation reactions.

[0008] Polyethylene glycol-phosphorylcholine has a unique molecular structure and properties. Its polyethylene glycol chain segment has good solubility and flexibility, which can significantly increase the fluidity and collision probability of reactant molecules in the reaction system, thereby promoting the approach and reaction of salidroside and methyl glucoside, increasing the rate of etherification reaction, shortening the reaction time, and improving production efficiency. In the etherification reaction process, the generated reaction intermediates usually have high activity and instability. Polyethylene glycol-phosphorylcholine forms specific interactions with these intermediates (such as hydrogen bonds and electrostatic effects) to effectively stabilize the intermediates, allowing them to participate in subsequent reactions more smoothly, reducing the possibility of intermediate decomposition or side reactions, thereby improving the selectivity of the reaction and the yield of the product. In addition, polyethylene glycol-phosphorylcholine can form a protective film-like structure around the product molecules, and through steric hindrance and physical shielding effects, it reduces the influence of external environmental factors (such as oxygen, moisture, light, etc.) on the product, and reduces the possibility of oxidation, hydrolysis, degradation and other reactions of the product, thereby improving the chemical stability and storage stability of the product.

[0009] S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 50-60°C at a speed of 100-150rpm, and then placed in a vacuum drying oven for drying to obtain etherified salidroside.

[0010] Preferably, in S1.2, the concentration of the potassium carbonate solution is 5%-10%.

[0011] Preferably, in S1.2, the pH value of the potassium carbonate solution is adjusted to 9-10.

[0012] Preferably, in S1.2, the polyethylene glycol-phosphorylcholine is slowly added at a rate of 1-2 parts by weight per minute.

[0013] Preferably, in S1.3, the vacuum drying temperature is 40-50°C, and the drying time is 4-8h.

[0014] On the other hand, the present invention provides a method for preparing an anti-fatigue, high-efficiency, and sugar-reduced sports drink based on biotechnology, which is used for any of the above-mentioned anti-fatigue, high-efficiency, and sugar-reduced sports drinks based on biotechnology, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of hawthorn extract, 6-12 parts by weight of green tea extract, 3-8 parts by weight of guarana extract, 8-15 parts by weight of wolfberry extract, 20-30 parts by weight of erythritol, 1-3 parts by weight of sodium chloride, 0.5-2 parts by weight of potassium chloride, 2-5 parts by weight of acidulant, 1-3 parts by weight of γ-aminobutyric acid, 1-3 parts by weight of L-ornithine, 0.5-2 parts by weight of coenzyme Q10, 0.5-2 parts by weight of choline, and 0.5-2 parts by weight of sodium carboxymethyl cellulose; S2.2, add 60-70% water into a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 100-150 rpm for 10-15 min at 15-20°C; then add erythritol, sodium chloride and potassium chloride, and continue stirring at 100-150 rpm for 5-10 min to obtain a mixed solution; Hawthorn extract contains a large amount of organic acids and dietary fiber, which can promote gastrointestinal motility and aid digestion. Its flavonoids also have cardiovascular protective effects. The caffeine in green tea extract can excite the central nervous system and reduce sports fatigue, and tea polyphenols have strong antioxidant capacity. The caffeine and natural sugars in guarana extract can provide energy and enhance endurance. The polysaccharides in wolfberry extract can regulate the body's immune function, and a variety of antioxidants can scavenge free radicals and replenish nutrients lost during exercise.

[0015] S2.3, add the acidulant to the mixed solution, stir at a speed of 100-200 rpm for 5-10 minutes, and adjust the pH to 3.5-4.5; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir at a speed of 100-200 rpm for 10-15 minutes until they are evenly distributed; S2.4. Add sodium carboxymethyl cellulose solution to the mixed solution in S2.3, and stir at a speed of 200-300 rpm for 15-30 minutes; then add 30-40% of water, and stir at a speed of 300-400 rpm for 10-15 minutes; after mixing evenly, filter, and then homogenize with a high-pressure homogenizer to obtain an anti-fatigue, high-efficiency, and sugar-reducing sports drink based on biotechnology.

[0016] Preferably, in S2.3, the acidulant is any one of citric acid or isocitric acid.

[0017] Preferably, in S2.4, the sodium carboxymethyl cellulose solution is prepared by dissolving sodium carboxymethyl cellulose in water at 60-70° C. to obtain a sodium carboxymethyl cellulose solution with a concentration of 1%-2%.

[0018] Preferably, in S2.4, the pressure of the high-pressure homogenizer is 50-60 MPa, and the number of cycles is 1-3 times.

[0019] Compared with the prior art, the present invention has the following beneficial effects: 1. In this anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology and its preparation method, methyl glucoside is added. Since it can undergo an etherification reaction with salidroside, the generated etherification product is not easy to decompose the effective ingredients when exposed to light and temperature changes, which is beneficial to the long-term preservation of the beverage; in addition, the energy generated by the hydrolysis of methyl glucoside can supplement exercise consumption, and synergize with the anti-fatigue effect of salidroside to relieve sports fatigue more quickly and improve physical function; the product after the reaction can also improve the taste, bring a mild sweetness to the beverage, and optimize the overall flavor.

[0020] 2. In this anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology and its preparation method, polyethylene glycol-phosphorylcholine is added. Since it can form a structure similar to a protective film around the product molecules, it can reduce the impact of external environmental factors (such as oxygen, moisture, light, etc.) on the product through steric hindrance effect and physical shielding effect, and reduce the possibility of oxidation, hydrolysis, degradation and other reactions of the product, thereby improving the chemical stability and storage stability of the product. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] The invention provides an anti-fatigue and high-efficiency sugar-reducing sports drink based on biotechnology, comprising the following components: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of hawthorn extract, 6-12 parts by weight of green tea extract, 3-8 parts by weight of guarana extract, 8-15 parts by weight of wolfberry extract, 20-30 parts by weight of erythritol, 1-3 parts by weight of sodium chloride, 0.5-2 parts by weight of potassium chloride, 2-5 parts by weight of acidulant, 1-3 parts by weight of gamma-aminobutyric acid, 1-3 parts by weight of L-ornithine, 0.5-2 parts by weight of coenzyme Q10, 0.5-2 parts by weight of choline and 0.5-2 parts by weight of sodium carboxymethyl cellulose; The etherified salidroside is prepared by etherifying salidroside with methyl glucoside and adding polyethylene glycol-phosphorylcholine.

[0023] The acidulant is any one of citric acid or isocitric acid, preferably isocitric acid.

[0024] The main active ingredients in hawthorn extract include flavonoids (quercetin, rutin, hyperoside, etc.), organic acids (maslinic acid, ursolic acid, oleanolic acid, etc.), triterpenes (maslin diol, maslin triol, etc.) and polysaccharides.

[0025] The main active ingredients in green tea extract include tea polyphenols (catechins), caffeine and theanine.

[0026] The main active ingredients in guarana extract include caffeine, tannic acid, ginsenosides, amino acids and minerals.

[0027] The main active ingredients in wolfberry extract include wolfberry polysaccharides, carotenoids (β-carotene, zeaxanthin, etc.), flavonoids (quercetin, rutin, etc.) and alkaloids (betaine).

[0028] Example 1: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 80 parts by weight of water, 5 parts by weight of etherified salidroside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of wolfberry extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 parts by weight of potassium chloride, 2 parts by weight of isocitric acid, 1 part by weight of γ-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 parts by weight of coenzyme Q10, 0.5 parts by weight of choline, and 0.5 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0029] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 18 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0030] Example 2: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 80 parts by weight of water, 5 parts by weight of etherified salidroside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of wolfberry extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 parts by weight of potassium chloride, 2 parts by weight of isocitric acid, 1 part by weight of γ-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 parts by weight of coenzyme Q10, 0.5 parts by weight of choline, and 0.5 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0031] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 21 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0032] Example 3: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 80 parts by weight of water, 5 parts by weight of etherified salidroside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of wolfberry extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 parts by weight of potassium chloride, 2 parts by weight of isocitric acid, 1 part by weight of γ-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 parts by weight of coenzyme Q10, 0.5 parts by weight of choline, and 0.5 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0033] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 24 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0034] Example 4: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 80 parts by weight of water, 5 parts by weight of etherified salidroside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of wolfberry extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 parts by weight of potassium chloride, 2 parts by weight of isocitric acid, 1 part by weight of γ-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 parts by weight of coenzyme Q10, 0.5 parts by weight of choline, and 0.5 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0035] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 27 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0036] Example 5: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 90 parts by weight of water, 5 parts by weight of etherified salidroside, 11 parts by weight of hawthorn extract, 9 parts by weight of green tea extract, 5 parts by weight of guarana extract, 11 parts by weight of wolfberry extract, 25 parts by weight of erythritol, 2 parts by weight of sodium chloride, 1.2 parts by weight of potassium chloride, 3.5 parts by weight of isocitric acid, 2 parts by weight of γ-aminobutyric acid, 2 parts by weight of L-ornithine, 1.2 parts by weight of coenzyme Q10, 1.2 parts by weight of choline, and 1.2 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0037] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 24 parts by weight of methyl glucoside, 6 parts by weight of polyethylene glycol-phosphorylcholine, and 8 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0038] Example 6: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 90 parts by weight of water, 8 parts by weight of etherified salidroside, 11 parts by weight of hawthorn extract, 9 parts by weight of green tea extract, 5 parts by weight of guarana extract, 11 parts by weight of wolfberry extract, 25 parts by weight of erythritol, 2 parts by weight of sodium chloride, 1.2 parts by weight of potassium chloride, 3.5 parts by weight of isocitric acid, 2 parts by weight of γ-aminobutyric acid, 2 parts by weight of L-ornithine, 1.2 parts by weight of coenzyme Q10, 1.2 parts by weight of choline, and 1.2 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0039] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 24 parts by weight of methyl glucoside, 6 parts by weight of polyethylene glycol-phosphorylcholine, and 8 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0040] Example 7: A biotechnology-based anti-fatigue, high-efficiency, sugar-reducing sports drink and a preparation method thereof, comprising the following steps: S2.1. Weigh the following components in parts by weight respectively: 90 parts by weight of water, 10 parts by weight of etherified salidroside, 11 parts by weight of hawthorn extract, 9 parts by weight of green tea extract, 5 parts by weight of guarana extract, 11 parts by weight of wolfberry extract, 25 parts by weight of erythritol, 2 parts by weight of sodium chloride, 1.2 parts by weight of potassium chloride, 3.5 parts by weight of isocitric acid, 2 parts by weight of γ-aminobutyric acid, 2 parts by weight of L-ornithine, 1.2 parts by weight of coenzyme Q10, 1.2 parts by weight of choline, and 1.2 parts by weight of sodium carboxymethyl cellulose; S2.2, add 70% water to a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 150 rpm for 15 min at 20°C; then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 150 rpm for 10 min to obtain a mixed solution; S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at 200 rpm for 15 min until evenly distributed; S2.4. Add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 300 rpm for 30 min. Then add 30% water and stir at 400 rpm for 15 min. After mixing evenly, filter and homogenize with a high-pressure homogenizer at a pressure of 60 MPa for 3 cycles to obtain an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology.

[0041] Wherein, the preparation method of etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 15 parts by weight of salidroside, 24 parts by weight of methyl glucoside, 6 parts by weight of polyethylene glycol-phosphorylcholine, and 8 parts by weight of potassium carbonate; S1.2. Under nitrogen protection, salidroside and methyl glucoside were dissolved in deionized water, stirred at 200 rpm for 40 min, and a 5% potassium carbonate solution was added to adjust the pH value of the solution to 9; polyethylene glycol-phosphorylcholine was slowly added at a rate of 2 parts by weight per minute, and the reaction system was placed in a constant temperature water bath at 60°C, and stirred at 400 rpm for 20 h; S1.3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150rpm, and then placed in a vacuum drying oven for drying at a drying temperature of 50°C and a drying time of 8h to obtain etherified salidroside.

[0042] Comparative Example 1 The method of Example 7 was adopted, but etherified salidroside was not used. Salidroside was directly used to prepare an anti-fatigue and high-efficiency sugar-reducing sports drink based on biotechnology.

[0043] Comparative Example 2 The method of Example 7 is adopted to remove polyethylene glycol-phosphorylcholine in the preparation method of etherified salidroside.

[0044] The present invention discloses an anti-fatigue, high-efficiency, sugar-reducing sports beverage based on biotechnology prepared by etherified salidroside, wherein the performance index inspection items and inspection standards of the anti-fatigue, high-efficiency, sugar-reducing sports beverage are as follows: The anti-fatigue effect is determined through animal experiments: rats are selected, and after being given a certain dose of sports drink, the animals are allowed to exercise vigorously for 30 minutes. Blood samples are collected immediately after the exercise, and serum or plasma is separated using a centrifuge. The blood lactate concentration is determined using an enzymatic method; a lower blood lactate concentration is more likely to indicate that a substance has a better anti-fatigue effect.

[0045] Dilute the sample in a certain proportion, remove insoluble substances by filtration, and prepare a series of standard solutions of known concentrations to cover the expected sample concentration range; use a high performance liquid chromatography system to analyze the standard solution, record the peak area or peak height, and draw a standard curve; inject the treated sample into the HPLC system, record the chromatogram and peak area or peak height; calculate the concentration of the active ingredient in the sample based on the standard curve.

[0046] According to the above standards, the anti-fatigue high-efficiency sugar-reduced sports drinks prepared in the above Examples 1-7 and Comparative Examples 1-2 were tested, and the obtained data are shown in Table 1: Table 1 Performance data of anti-fatigue high-efficiency sugar-reduced sports drinks of Examples 1-7 and Comparative Examples 1-2 It can be seen from Examples 1-4 that: when the mass ratio of salidroside to methyl glucoside in the etherified salidroside gradually increases, the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reduced sports drink gradually increase, but when the mass ratio of salidroside to methyl glucoside reaches a certain value, the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reduced sports drink gradually decrease. It can be seen that with the increase of the mass ratio of methyl glucoside, the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reduced sports drink are improved, but excessive increase may reduce the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reduced sports drink; Salidroside is the main active ingredient of Rhodiola rosea, with a unique chemical structure and physiological activity. It can promote the body's utilization of oxygen, improve the energy metabolism level of cells, enhance the function of mitochondria, and promote the synthesis of adenosine triphosphate, thereby providing more energy for muscle contraction and body movement; in addition, salidroside also has a significant antioxidant effect, which can increase the activity of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase, reduce the content of lipid peroxidation products such as malondialdehyde, and reduce the damage of free radicals to cells; methyl glucoside, as another active ingredient in Rhodiola rosea, can participate in the sugar metabolism process, optimize the synthesis and decomposition of glycogen, maintain the stability of blood sugar levels, and ensure a continuous supply of energy during exercise.

[0047] As the mass ratio of salidroside to methyl glucoside increases, their regulatory effects on energy metabolism become more significant, which can better meet the body's energy needs during exercise and delay the onset of fatigue; a large number of free radicals will be produced during exercise, and excessive free radicals will attack the biological macromolecules in the cells, causing cell function damage and inducing fatigue; salidroside can effectively scavenge free radicals and reduce oxidative stress damage by increasing the activity of antioxidant enzymes and reducing the content of lipid peroxidation products, thereby improving the anti-fatigue effect; methyl glucoside may also have a certain antioxidant capacity or synergize with salidroside to enhance the function of the antioxidant system; when the mass ratio of the two increases, the overall antioxidant capacity of the sports drink is enhanced, which can effectively scavenge free radicals and reduce oxidative stress damage, thereby improving the anti-fatigue effect.

[0048] Furthermore, by comparing Examples 5-7, it can be seen that when other components remain unchanged, only when the proportion of etherified salidroside gradually increases, the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reducing sports drink gradually increase. It can be seen that etherified salidroside may have a certain stabilizing effect on other active ingredients in the beverage. When its proportion increases, it can better protect other active ingredients and reduce their degradation or inactivation during storage and use, thereby keeping the total amount of active ingredients that can play a role in the beverage at a higher level; etherified salidroside may have a stronger anti- Oxidative properties: as its proportion increases, it can more effectively remove free radicals in the body, reduce oxidative damage, and protect the normal functions of cells and tissues; in addition, the increase in the proportion of etherified salidroside may have a more significant regulatory effect on the neuroendocrine system. It may be able to regulate neuroendocrine pathways such as the hypothalamus-pituitary-adrenal axis, so that the body can better adapt to the stress state during exercise and maintain a stable internal environment; at the same time, it may also affect the secretion and transmission of neurotransmitters, such as increasing the release of dopamine, norepinephrine, etc., improving the excitability of the brain and the body's motor ability, and reducing fatigue.

[0049] From the comparison between Example 7 and Comparative Example 1, it can be seen that when etherified salidroside is not used and salidroside is directly used to prepare the anti-fatigue and high-efficiency sugar-reduced sports drink based on biotechnology, the active ingredient content and anti-fatigue effect in the anti-fatigue and high-efficiency sugar-reduced sports drink are significantly reduced.

[0050] Taking Example 7 as the optimal example and combining it with Comparative Example 2, it can be seen that in the preparation method of etherified salidroside, when polyethylene glycol-phosphorylcholine is removed, the content of active ingredients and the anti-fatigue effect in the anti-fatigue high-efficiency sugar-reduced sports drink are significantly reduced; Polyethylene glycol-phosphorylcholine may act as a reaction intermediate or catalyst in the synthesis reaction of etherified salidroside, which helps to improve the reaction rate and completeness, thereby increasing the production of etherified salidroside. Its presence may protect the stability of etherified salidroside, reduce the occurrence of side reactions such as degradation or isomerization, and ensure the content of the final active ingredient; Polyethylene glycol-phosphorylcholine may also promote the absorption of etherified salidroside, and it can help etherified salidroside better pass through the gastrointestinal mucosa into the blood circulation, thereby improving its absorption efficiency and anti-fatigue effect in the body; in addition, polyethylene glycol-phosphorylcholine itself has a certain antioxidant capacity, or it can synergistically enhance the antioxidant effect with etherified salidroside, more effectively scavenge free radicals during exercise, and reduce oxidative damage; after removing polyethylene glycol-phosphorylcholine, the production and absorption efficiency of etherified salidroside will decrease, and the anti-fatigue effect will be significantly weakened; at the same time, the lack of its antioxidant effect may lead to a decrease in the body's ability to scavenge free radicals, increased oxidative stress, and easy to cause fatigue.

[0051] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology, characterized in that: The invention comprises the following components: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of hawthorn extract, 6-12 parts by weight of green tea extract, 3-8 parts by weight of guarana extract, 8-15 parts by weight of wolfberry extract, 20-30 parts by weight of erythritol, 1-3 parts by weight of sodium chloride, 0.5-2 parts by weight of potassium chloride, 2-5 parts by weight of acidulant, 1-3 parts by weight of gamma-aminobutyric acid, 1-3 parts by weight of L-ornithine, 0.5-2 parts by weight of coenzyme Q10, 0.5-2 parts by weight of choline and 0.5-2 parts by weight of sodium carboxymethyl cellulose; The etherified salidroside is prepared by etherifying salidroside with methyl glucoside and adding polyethylene glycol-phosphorylcholine.

2. The anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 1, characterized in that: The preparation method of the etherified salidroside is as follows: S1.1, weigh the following components in parts by weight respectively: 10-20 parts by weight of salidroside, 15-30 parts by weight of methyl glucoside, 3-8 parts by weight of polyethylene glycol-phosphorylcholine, and 5-12 parts by weight of potassium carbonate; S1.

2. Under nitrogen protection, dissolve salidroside and methyl glucoside in deionized water, stir at 100-200 rpm for 30-60 min, and add potassium carbonate solution to adjust the pH value of the solution; slowly add polyethylene glycol-phosphorylcholine, and place the reaction system in a constant temperature water bath at 40-60°C, and stir at 300-400 rpm for 10-24 h; S1.

3. After the reaction is completed, the reaction solution is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 50-60°C at a speed of 100-150rpm, and then placed in a vacuum drying oven for drying to obtain etherified salidroside.

3. The anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 2, characterized in that: In S1.2, the concentration of the potassium carbonate solution is 5%-10%.

4. The anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 2, characterized in that: In S1.2, the pH value of the solution is adjusted to 9-10 by potassium carbonate.

5. The anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 2, characterized in that: In S1.2, the polyethylene glycol-phosphorylcholine is slowly added at a speed of 1-2 parts by weight per minute.

6. The anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 2, characterized in that: In S1.3, the vacuum drying temperature is 40-50° C. and the drying time is 4-8 h.

7. The method for preparing an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to any one of claims 1 to 6, characterized in that: S2.

1. Weigh the following components in parts by weight respectively: 80-100 parts by weight of water, 5-10 parts by weight of etherified salidroside, 8-15 parts by weight of hawthorn extract, 6-12 parts by weight of green tea extract, 3-8 parts by weight of guarana extract, 8-15 parts by weight of wolfberry extract, 20-30 parts by weight of erythritol, 1-3 parts by weight of sodium chloride, 0.5-2 parts by weight of potassium chloride, 2-5 parts by weight of acidulant, 1-3 parts by weight of γ-aminobutyric acid, 1-3 parts by weight of L-ornithine, 0.5-2 parts by weight of coenzyme Q10, 0.5-2 parts by weight of choline, and 0.5-2 parts by weight of sodium carboxymethyl cellulose; S2.2, add 60-70% water into a container, add etherified salidroside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence, and stir at 100-150 rpm for 10-15 min at 15-20°C; then add erythritol, sodium chloride and potassium chloride, and continue stirring at 100-150 rpm for 5-10 min to obtain a mixed solution; S2.3, add the acidulant to the mixed solution, stir at a speed of 100-200 rpm for 5-10 minutes, and adjust the pH to 3.5-4.5; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir at a speed of 100-200 rpm for 10-15 minutes until they are evenly distributed; S2.

4. Add sodium carboxymethyl cellulose solution to the mixed solution in S2.3, and stir at a speed of 200-300 rpm for 15-30 minutes; then add 30-40% of water, and stir at a speed of 300-400 rpm for 10-15 minutes; after mixing evenly, filter, and then homogenize with a high-pressure homogenizer to obtain an anti-fatigue, high-efficiency, and sugar-reducing sports drink based on biotechnology.

8. The method for preparing an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 7, characterized in that: In S2.3, the acidulant is any one of citric acid or isocitric acid.

9. The method for preparing an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 7, characterized in that: In S2.4, the sodium carboxymethyl cellulose solution is prepared by dissolving sodium carboxymethyl cellulose in water at 60-70° C. to obtain a sodium carboxymethyl cellulose solution with a concentration of 1%-2%.

10. The method for preparing an anti-fatigue, high-efficiency, sugar-reducing sports drink based on biotechnology according to claim 7, characterized in that: In S2.4, the pressure of the high-pressure homogenizer is 50-60 MPa, and the number of cycles is 1-3 times.

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