Medicinal and edible composition with effect of preventing myocardial infarction and cerebral infarction and preparation method of medicinal and edible composition

Abstract

The invention relates to a medicinal and edible composition with the effect of preventing myocardial infarction and cerebral infarction and a preparation method thereof, and belongs to the technical field of health-care food, and the preparation method comprises the following steps: uniformly mixing a ginkgo biloba extract and a spina date seed extract to obtain a traditional Chinese medicine extract; after the probiotics are activated, activated probiotics are obtained; the traditional Chinese medicine extract, the prebiotics and the activated probiotics are subjected to fermentation treatment and then mixed with the stabilizer, and the medicinal and edible composition with the effect of preventing myocardial infarction and cerebral infarction is obtained through filtration and freeze drying. According to the technical scheme, the ginkgo biloba extract and the spina date seed extract are mixed, high antioxidant activity is achieved, after the probiotics are activated, the probiotics, the traditional Chinese medicine extract and the prebiotics are subjected to fermentation treatment, intestinal health can be maintained, myocardial infarction and cerebral infarction can be prevented, the bioavailability of the traditional Chinese medicine extract can be well improved, and the health-care effect is achieved. The stability is enhanced, so that the prepared medicinal and edible composition has a relatively good effect in the aspect of preventing myocardial infarction and cerebral infarction.

Description

Technical Field

[0001] The present invention belongs to the technical field of health food, and in particular, relates to a medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction and a preparation method thereof. Background Art

[0002] With the development of the national economy, people's material conditions are getting better and better, and their diets are becoming increasingly rich. Due to the rich variety of diets, uncontrolled diets, overnutrition, and some bad living habits, the probability of people suffering from diseases such as arteriosclerosis, heart disease, myocardial infarction, cerebral infarction, stroke, hypertension, and tumors is gradually increasing. Cerebral infarction refers to ischemic necrosis or cerebral softening of localized brain tissue caused by obstruction of blood supply, ischemia, and hypoxia in the brain. Myocardial infarction refers to acute myocardial ischemia and necrosis, which is mostly caused by a sharp reduction or interruption of coronary artery blood supply on the basis of coronary artery lesions, resulting in severe and persistent acute ischemia of the corresponding myocardium, and belongs to acute coronary syndrome. In the prevention of myocardial infarction and cerebral infarction, traditional Chinese medicine occupies a very important position, but most of the current traditional Chinese medicine preparations have general effects, and there are also defects such as inconvenience in taking and potential side effects. Therefore, there is an urgent need for a new and more effective medicine-food combination to play an important role in the preparation of health foods for the prevention of myocardial infarction and cerebral infarction.

[0003] At present, food-and-medicine compositions are usually prepared by mixing a variety of traditional Chinese medicines. This method makes the composition more complicated and the quality difficult to control. In severe cases, it is easy to cause adverse reactions in the human body. In addition, the active ingredients in the composition cannot be effectively extracted and retained, resulting in poor stability and low bioavailability. The preventive effect on myocardial infarction and cerebral infarction is also poor. Therefore, it is necessary to prepare a food-and-medicine composition with higher utilization rate and better effect for preventing myocardial infarction and cerebral infarction. Summary of the invention

[0004] The purpose of the present invention is to provide a medicine-food composition with the effect of preventing myocardial infarction and cerebral infarction and a preparation method thereof. The Chinese medicine extract is obtained by uniformly mixing the ginkgo leaf extract and the spinach seed extract; the ginkgo leaf extract is extracted by ultrasound-assisted enzyme, and the spinach seed extract is extracted by ultrasound-assisted ethanol, which improves the extraction efficiency and yield of the ginkgo leaf extract and the spinach seed extract, and has high antioxidant activity. The two play a synergistic role, can reduce blood lipid levels, and effectively prevent atherosclerosis; after activating probiotics, activated probiotics are obtained; the probiotics can better regulate the balance of intestinal flora and inhibit The growth and reproduction of harmful bacteria; the Chinese herbal medicine extract, prebiotics, and activated probiotics are fermented and then mixed with a stabilizer, filtered, and freeze-dried to obtain a medicine-food composition with the effect of preventing myocardial infarction and cerebral infarction; prebiotics not only have the functions of regulating blood sugar and blood pressure and lowering cholesterol, but also can provide nutrients for probiotics. The Chinese herbal medicine extract, prebiotics, and activated probiotics are fermented to improve the bioavailability of the Chinese herbal medicine extract, enhance its stability, help maintain intestinal health and prevent diseases such as myocardial infarction and cerebral infarction. The prepared medicine-food composition has a good preventive effect in preventing myocardial infarction and cerebral infarction, and has less toxic and side effects.

[0005] Technical problem to be solved by the present invention: At present, the medicine-food combination is usually prepared by mixing multiple traditional Chinese medicines. This method makes the composition of the combination more complicated and the quality is difficult to control. In severe cases, it is easy to cause adverse reactions in the human body. In addition, the active ingredients in the composition cannot be effectively extracted and retained, resulting in poor stability and low bioavailability. The preventive effect on myocardial infarction and cerebral infarction is also poor. Therefore, it is necessary to prepare a medicine-food combination with higher utilization rate and better effect for preventing myocardial infarction and cerebral infarction.

[0006] The purpose of the present invention can be achieved by the following technical solutions: A method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction comprises the following steps: S1: Evenly mix the ginkgo leaf extract and the spinosa jujube seed extract to obtain a Chinese medicine extract; S2: activating the probiotics to obtain activated probiotics; S3: The Chinese herbal extract, prebiotics and the composite bacterial solution in step S2 are fermented and then mixed with a stabilizer, and filtered and freeze-dried to obtain a medicine-food composition with the efficacy of preventing myocardial infarction and cerebral infarction.

[0007] Furthermore, in step S1, the method for preparing the ginkgo leaf extract comprises the following steps: The pretreated ginkgo leaf powder is immersed in a polyethylene glycol aqueous solution, and then a complex enzyme is added and extracted by ultrasonic treatment. After filtration, an extract is obtained, and the extract is concentrated by reduced pressure distillation at 40-50°C, and then anhydrous ethanol is added to precipitate the concentrate. The obtained precipitate is centrifuged for 15-25 minutes, washed with anhydrous ethanol, and freeze-dried to obtain a ginkgo leaf extract.

[0008] Furthermore, the mass ratio of the pretreated ginkgo leaf powder to the polyethylene glycol aqueous solution is 4.8-5.2:140-160.

[0009] Furthermore, the ginkgo leaf powder is obtained by drying the ginkgo leaves at 50° C. to a constant weight, then grinding the leaves and passing through an 80-mesh sieve.

[0010] Furthermore, the complex enzyme is prepared by mixing cellulase, pectinase and trypsin in a mass ratio of 3:2:1.

[0011] Furthermore, the ultrasonic treatment: the ultrasonic temperature is 50-60°C, and the ultrasonic time is 25-30min.

[0012] Furthermore, in step S1, the method for preparing the spiny jujube seed extract comprises the following steps: The pretreated spiny jujube seed powder is added to an ethanol aqueous solution and extracted by ultrasonic treatment. After filtering, the obtained filtrate is concentrated by vacuum distillation at 45-55° C., then added to ethanol for precipitation, washed with deionized water, and freeze-dried to obtain the spiny jujube seed extract.

[0013] Furthermore, the mass ratio of the pretreated spinosa jujube seed powder to the ethanol aqueous solution is 0.8-1.2:10-20.

[0014] Furthermore, the wild jujube seed powder is obtained by grinding the wild jujube seeds and then passing through a 60-mesh sieve.

[0015] Furthermore, the ultrasonic treatment: the ultrasonic temperature is 60-70°C, and the ultrasonic time is 50-60min.

[0016] Furthermore, step S2 is specifically as follows: The probiotics were streaked onto MRS solid culture medium, and then cultured at 36-38°C for 36-48 hours to obtain single colonies, and then single colonies were picked and inoculated into MRS liquid culture medium, and anaerobically cultured at 35-38°C for 18-24 hours until colonies grew to obtain activated probiotics.

[0017] Furthermore, the probiotics are obtained by uniformly mixing Bifidobacterium longum, Lactobacillus acidophilus and Akkermansia muciniphila in a live bacteria count ratio of 1:2-4:1.

[0018] Furthermore, the components of the MRS solid culture medium include: 5-10 g / L peptone, 5-10 g / L beef extract, 3-5 g / L yeast extract, 15-20 g / L glucose, 1-2 g / L anhydrous sodium acetate, 1.5-2 g / L diammonium hydrogen citrate, 2-3 g / L K2HPO4·3H2O, 0.3-0.5 g / L MgSO4·7H2O, 0.2-0.3 g / L MnSO4·H2O, 0.5-1 g / L Tween 80, 15-20 g / L agar, and 1000 g / L distilled water.

[0019] Furthermore, the components of the MRS liquid culture medium include: 5-10 g / L peptone, 5-10 g / L beef extract, 3-5 g / L yeast extract, 15-20 g / L glucose, 1-2 g / L anhydrous sodium acetate, 1.5-2 g / L diammonium hydrogen citrate, 2-3 g / L K2HPO4·3H2O, 0.3-0.5 g / L MgSO4·7H2O, 0.2-0.3 g / L MnSO4·H2O, 0.5-1 g / L Tween 80, and 1000 g / L distilled water.

[0020] Furthermore, the inoculation amount of the single colony is 5%.

[0021] Furthermore, step S3 is specifically as follows: The Chinese herbal extract and prebiotics in step S1 are added to deionized water, stirred evenly, and then sterilized for 15-20 minutes to obtain a fermentation matrix, the activated probiotics in step S2 are inoculated into the fermentation matrix, and fermentation treatment is performed to obtain a fermentation liquid, the fermentation liquid is mixed with a stabilizer, stirred for 20-30 minutes, microfiltered through a filter membrane, and freeze-dried for 24-36 hours to obtain a medicine-food composition with the effect of preventing myocardial infarction and cerebral infarction.

[0022] Furthermore, the mass ratio of the traditional Chinese medicine extract, prebiotics and deionized water is 0.8-1.2:0.3-0.5:90-110.

[0023] Furthermore, the prebiotics are composed of a mixture of β-glucan and Dendrobium officinale polysaccharide in a mass ratio of 0.7-0.8:0.4-0.5.

[0024] Furthermore, in step S3, the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 2×10 9 -3×10 9 CFU / mL.

[0025] Furthermore, the fermentation process: the fermentation temperature is 36-38°C, and the fermentation time is 35-45h.

[0026] Furthermore, the mass ratio of the fermentation liquid to the stabilizer is 100:5-7.

[0027] Furthermore, the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol esters mixed in a mass ratio of 1-1.2:0.7-0.8:0.3-0.4.

[0028] Beneficial effects of the present invention: (1) In the technical scheme of the present invention, the Chinese medicine extract is obtained by uniformly mixing the ginkgo leaf extract and the spinach seed extract; the ginkgo leaf extract is extracted by ultrasound-assisted enzyme, which can better improve the extraction efficiency and yield of the ginkgo leaf extract, maximize the retention of the effective components in the ginkgo leaf, and further improve the antioxidant activity of the ginkgo leaf extract; the spinach seed extract is extracted by ultrasound-assisted ethanol, which can also improve the extraction efficiency and yield of the spinach seed extract, so that the effective components in the spinach seed are better extracted and the antioxidant activity is improved. Apricot leaf extract and Chinese jujube seed extract both have good antioxidant activity, and the two play a synergistic role, which can lower blood lipid levels, reduce inflammatory responses, and have a good preventive and therapeutic effect on atherosclerosis; after the probiotics are activated, activated probiotics are obtained; the probiotics are composed of a mixture of Bifidobacterium longum, Lactobacillus acidophilus, and Akkermansia muciniphila, which are all beneficial bacteria. The three bacteria have a mutually promoting effect, can better regulate the microecological balance of intestinal flora, and can inhibit the growth and reproduction of harmful bacteria.

[0029] (2) In the technical scheme of the present invention, the Chinese herbal extract in step S1, prebiotics, and activated probiotics in step S2 are fermented and then mixed with a stabilizer, filtered, and freeze-dried to obtain a medicinal and edible composition with the effect of preventing myocardial infarction and cerebral infarction; the prebiotics are composed of a mixture of β-glucan and Dendrobium officinale polysaccharide, and the β-glucan and Dendrobium officinale polysaccharide play a synergistic role, which not only has the effects of regulating blood sugar and blood pressure, lowering cholesterol, resisting obesity, and improving atherosclerosis, but also can provide nutrients for probiotics and promote their growth and reproduction. The probiotics and prebiotics mixed together can have a stronger effect, which helps to maintain intestinal health and prevent diseases such as myocardial infarction and cerebral infarction; the Chinese herbal extract, prebiotics, and activated probiotics are fermented, and the activated probiotics are mixed with a stabilizer, filtered, and freeze-dried to obtain a medicinal and edible composition with the effect of preventing myocardial infarction and cerebral infarction. Chemically activated probiotics can convert macromolecules in Chinese medicine extracts into small molecules, making them easier to be absorbed and utilized by the human body, and activated probiotics can decompose toxic substances in Chinese medicine extracts, thereby reducing the toxic side effects of drugs. After fermentation, Chinese medicine extracts have stronger pharmacological effects, can reduce the metabolic losses of Chinese medicine extracts in the body, and improve the bioavailability of Chinese medicine extracts, so that they have better preventive effects in preventing myocardial infarction and cerebral infarction. By adding stabilizers, L-ascorbic acid, vitamin D2 and plant sterol esters have a synergistic stabilizing effect, which can not only further enhance the stability of Chinese medicine extracts, but also plant sterol esters can reduce the risk of atherosclerotic cardiovascular disease, so that the medicine-food composition can play a better preventive effect.

[0030] (3) In the technical scheme of the present invention, a Chinese medicine extract is obtained by uniformly mixing a ginkgo leaf extract and a spiny jujube seed extract; after activating the probiotics, an activated probiotic is obtained; the Chinese medicine extract, prebiotics, and activated probiotics are fermented and then mixed with a stabilizer to finally obtain a medicine-food composition with the efficacy of preventing myocardial infarction and cerebral infarction; the prepared medicine-food composition not only has a high bioavailability and good stability, but can also effectively inhibit the formation of atherosclerosis, and has a good preventive effect in preventing myocardial infarction and cerebral infarction; at the same time, the medicine-food composition has less toxic and side effects, and has a good application in the preparation of health food for preventing myocardial infarction and cerebral infarction. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The specific parameters of the raw materials used in the present invention are as follows: Ginkgo biloba leaves were provided by Bozhou Wufang Jiuru Pharmaceutical Co., Ltd.; Ziziphus jujuba seeds, batch number: 230407, were provided by Shanghai Kangqiao Chinese Medicine Pieces Co., Ltd.; Bifidobacterium longum, number: CICC6213, were purchased from China Industrial Microbiological Culture Collection Administration Center; Lactobacillus acidophilus, number: CICC 6081, were purchased from China Industrial Microbiological Culture Collection Administration Center; Akkermansia muciniphila, number: CICC 24917, purchased from China Industrial Microbiological Culture Collection Management Center; β-glucan, CAS No.: 9012-72-0, provided by Shaanxi Chenming Biotechnology Co., Ltd.; Dendrobium officinale polysaccharide, product number: TDT013, provided by Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd.; phytosterol esters, provided by Anhui Xinsheng Biotechnology Co., Ltd.; L-ascorbic acid, CAS No.: 50-81-7, provided by Shanghai McLean Biochemical Technology Co., Ltd.; vitamin D2, CAS No.: 50-14-6, provided by Shanghai McLean Biochemical Technology Co., Ltd.

[0033] The raw materials used in the following examples and comparative examples, the components of the MRS solid culture medium include: peptone 5-10g / L, beef extract 5-10g / L, yeast extract 3-5g / L, glucose 15-20g / L, anhydrous sodium acetate 1-2g / L, diammonium hydrogen citrate 1.5-2g / L, K2HPO4·3H2O2-3g / L, MgSO4·7H2O0.3-0.5g / L, MnSO4·H2O 0.2-0.3g / L, Tween 800.5-1g / L, agar 15-20g / L, and distilled water 1000g / L.

[0034] The components of MRS liquid culture medium include: peptone 5-10g / L, beef extract 5-10g / L, yeast extract 3-5g / L, glucose 15-20g / L, anhydrous sodium acetate 1-2g / L, diammonium hydrogen citrate 1.5-2g / L, K2HPO4·3H2O2-3g / L, MgSO4·7H2O0.3-0.5g / L, MnSO4·H2O 0.2-0.3g / L, Tween 800.5-1g / L, and distilled water 1000g / L.

[0035] Example 1 The preparation method of the medicine-food homologous composition having the effect of preventing myocardial infarction and cerebral infarction comprises the following steps: S1: Evenly mix the ginkgo leaf extract and the spinosa jujube seed extract to obtain a Chinese medicine extract; The preparation method of ginkgo leaf extract comprises the following steps: According to the mass ratio of the pretreated ginkgo leaf powder to the polyethylene glycol aqueous solution of 4.8:140, the pretreated ginkgo leaf powder was immersed in a 15wt% polyethylene glycol aqueous solution, and then a composite enzyme (the mass of the composite enzyme was 0.6% of the mass of the polyethylene glycol aqueous solution) was added, and extraction was performed by ultrasonic treatment (ultrasonic power of 100 W, ultrasonic frequency of 40 kHz), the ultrasonic treatment temperature was 50°C, the ultrasonic treatment time was 25 min, and after filtration, an extract was obtained, and it was concentrated to one third of the initial volume by reduced pressure distillation at 40°C, and then Anhydrous ethanol was added to precipitate the concentrate (the mass of anhydrous ethanol was 30% of the mass of the polyethylene glycol aqueous solution), the obtained precipitate was centrifuged at 3500 rpm for 25 minutes, washed 3 times with anhydrous ethanol (the mass of anhydrous ethanol each time was 10% of the mass of the polyethylene glycol aqueous solution), and freeze-dried at minus 15°C to obtain a ginkgo leaf extract, wherein the ginkgo leaf powder was obtained by drying the ginkgo leaf at 50°C to a constant weight, then grinding it, and passing it through an 80-mesh sieve; the composite enzyme was obtained by mixing cellulase, pectinase, and trypsin in a mass ratio of 3:2:1; The preparation method of the Chinese date seed extract comprises the following steps: According to the mass ratio of the pretreated spinach seed powder and the ethanol aqueous solution being 0.8:10, the pretreated spinach seed powder was added into a 70wt% ethanol aqueous solution, and extracted by ultrasonic treatment (ultrasonic power of 300W, ultrasonic frequency of 40kHz), the ultrasonic temperature was 60℃, the ultrasonic time was 50min, after filtration, the obtained filtrate was concentrated by vacuum distillation at 45℃, then added into ethanol for precipitation (the mass of ethanol was 50% of the mass of the ethanol aqueous solution), washed with deionized water for 3 times (the mass of deionized water each time was equal to the mass of the ethanol aqueous solution), freeze-dried at minus 20℃, and the spinach seed extract was obtained, wherein the spinach seed powder was obtained by grinding the spinach seed and then passing through a 60-mesh sieve; S2: Streak the probiotics on an MRS solid culture medium, and then culture at 36°C for 36 hours to obtain a single colony, and then pick the single colony and inoculate it into an MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 35°C for 18 hours until the colony grows out to obtain activated probiotics, wherein the probiotics are obtained by mixing Bifidobacterium longum, Lactobacillus acidophilus, and Akkermansia muciniphila in a live bacteria count ratio of 1:2:1; S3: According to the mass ratio of Chinese herbal extract, prebiotics and deionized water of 0.8:0.3:90, the Chinese herbal extract and prebiotics in step S1 are added to deionized water, and stirred evenly, and then sterilized at 65°C for 15 minutes to obtain a fermentation matrix, and the composite bacterial solution in step S2 is inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 2×10 9CFU / mL, after fermentation treatment, the fermentation temperature is 36°C, the fermentation time is 35h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:5, the fermentation liquid and the stabilizer are mixed, and stirred for 20min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 30°C for 24h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide in a mass ratio of 0.7:0.4; the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester in a mass ratio of 1:0.7:0.3.

[0036] Example 2 The preparation method of the medicine-food homologous composition having the effect of preventing myocardial infarction and cerebral infarction comprises the following steps: S1: Evenly mix the ginkgo leaf extract and the spinosa jujube seed extract to obtain a Chinese medicine extract; The preparation method of ginkgo leaf extract comprises the following steps: According to the mass ratio of pretreated ginkgo leaf powder to polyethylene glycol aqueous solution of 5:150, the pretreated ginkgo leaf powder was immersed in a 15wt% polyethylene glycol aqueous solution, and then a composite enzyme (the mass of the composite enzyme was 0.6% of the mass of the polyethylene glycol aqueous solution) was added, and extraction was performed by ultrasonic treatment (ultrasonic power of 100 W, ultrasonic frequency of 40 kHz), the ultrasonic treatment temperature was 55°C, the ultrasonic treatment time was 28 min, and after filtration, an extract was obtained, and it was concentrated to one third of the initial volume by reduced pressure distillation at 45°C, and then added The concentrate was precipitated with anhydrous ethanol (the mass of anhydrous ethanol was 30% of the mass of the polyethylene glycol aqueous solution), the precipitate was centrifuged at 4000 rpm for 20 min, washed 3 times with anhydrous ethanol (the mass of anhydrous ethanol each time was 10% of the mass of the polyethylene glycol aqueous solution), and freeze-dried at -12°C to obtain a ginkgo leaf extract, wherein the ginkgo leaf powder was obtained by drying the ginkgo leaf at 50°C to a constant weight, then grinding it, and passing it through an 80-mesh sieve; the composite enzyme was obtained by mixing cellulase, pectinase, and trypsin in a mass ratio of 3:2:1; The preparation method of the Chinese date seed extract comprises the following steps: According to the mass ratio of the pretreated Chinese jujube seed powder to the ethanol aqueous solution being 1:15, the pretreated Chinese jujube seed powder was added to a 70wt% ethanol aqueous solution, and extracted by ultrasonic treatment (ultrasonic power of 300W, ultrasonic frequency of 40kHz), the ultrasonic temperature was 65°C, the ultrasonic time was 55min, after filtration, the obtained filtrate was concentrated by vacuum distillation at 50°C, then added to ethanol for precipitation (the mass of ethanol was 50% of the mass of the ethanol aqueous solution), washed 3 times with deionized water (the mass of deionized water each time was equal to the mass of the ethanol aqueous solution), freeze-dried at minus 15°C, and obtained the Chinese jujube seed extract, wherein the Chinese jujube seed powder was obtained by grinding the Chinese jujube seed and then passing it through a 60-mesh sieve; S2: Streak the probiotics on an MRS solid culture medium, and then culture at 37°C for 42 hours to obtain a single colony, and then pick the single colony and inoculate it into an MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 37°C for 21 hours until the colony grows out to obtain activated probiotics, wherein the probiotics are obtained by mixing Bifidobacterium longum, Lactobacillus acidophilus, and Akkermansia muciniphila in a live bacteria count ratio of 1:3:1; S3: According to the mass ratio of Chinese herbal extract, prebiotics and deionized water of 1:0.4:100, the Chinese herbal extract and prebiotics in step S1 are added to deionized water, and stirred evenly, and then sterilized at 62°C for 18 minutes to obtain a fermentation matrix, and the composite bacterial solution in step S2 is inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 2.5×10 9 CFU / mL, after fermentation treatment, the fermentation temperature is 37℃, the fermentation time is 40h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:6, the fermentation liquid and the stabilizer are mixed, and stirred for 25min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 25℃ for 30h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide in a mass ratio of 0.75:0.45; the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester in a mass ratio of 1.1:0.75:0.35.

[0037] Example 3 The preparation method of the medicine-food homologous composition having the effect of preventing myocardial infarction and cerebral infarction comprises the following steps: S1: Evenly mix the ginkgo leaf extract and the spinosa jujube seed extract to obtain a Chinese medicine extract; The preparation method of ginkgo leaf extract comprises the following steps: According to the mass ratio of the pretreated ginkgo leaf powder to the polyethylene glycol aqueous solution of 5.2:160, the pretreated ginkgo leaf powder was immersed in a 15wt% polyethylene glycol aqueous solution, and then a composite enzyme (the mass of the composite enzyme was 0.6% of the mass of the polyethylene glycol aqueous solution) was added, and extraction was performed by ultrasonic treatment (ultrasonic power of 100 W, ultrasonic frequency of 40 kHz), the ultrasonic treatment temperature was 60°C, the ultrasonic treatment time was 30 min, and after filtration, an extract was obtained, and it was concentrated to one third of the initial volume by reduced pressure distillation at 50°C, and then Anhydrous ethanol was added to precipitate the concentrate (the mass of anhydrous ethanol was 30% of the mass of the polyethylene glycol aqueous solution), the obtained precipitate was centrifuged at 4500 rpm for 15 minutes, washed 3 times with anhydrous ethanol (the mass of anhydrous ethanol each time was 10% of the mass of the polyethylene glycol aqueous solution), and freeze-dried at minus 10°C to obtain a ginkgo leaf extract, wherein the ginkgo leaf powder was obtained by drying the ginkgo leaf at 50°C to a constant weight, then grinding it, and passing it through an 80-mesh sieve; the composite enzyme was obtained by mixing cellulase, pectinase, and trypsin in a mass ratio of 3:2:1; The preparation method of the Chinese date seed extract comprises the following steps: According to the mass ratio of the pretreated Chinese jujube seed powder and the ethanol aqueous solution being 1.2:20, the pretreated Chinese jujube seed powder was added to a 70wt% ethanol aqueous solution, and extracted by ultrasonic treatment (ultrasonic power of 300W, ultrasonic frequency of 40kHz), the ultrasonic temperature was 70°C, the ultrasonic time was 60min, after filtration, the obtained filtrate was concentrated by vacuum distillation at 55°C, and then added to ethanol for precipitation (the mass of ethanol was 50% of the mass of the ethanol aqueous solution), washed with deionized water for 3 times (the mass of deionized water each time was equal to the mass of the ethanol aqueous solution), and freeze-dried at minus 10°C to obtain a Chinese jujube seed extract, wherein the Chinese jujube seed powder was obtained by grinding the Chinese jujube seed and then passing it through a 60-mesh sieve; S2: Streak the probiotics on an MRS solid culture medium, and then culture at 38°C for 48 hours to obtain a single colony, and then pick the single colony and inoculate it into an MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 38°C for 24 hours until the colony grows out to obtain activated probiotics, wherein the probiotics are obtained by mixing Bifidobacterium longum, Lactobacillus acidophilus, and Akkermansia muciniphila in a live cell count ratio of 1:4:1; S3: According to the mass ratio of Chinese herbal medicine extract, prebiotics and deionized water of 1.2:0.5:110, the Chinese herbal medicine extract and prebiotics in step S1 are added to deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide mixed in a mass ratio of 0.8:0.5; the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester mixed in a mass ratio of 1.2:0.8:0.4.

[0038] Comparative Example 1 The difference between this comparative example and Example 3 is that when preparing the medicine-food composition with the efficacy of preventing myocardial infarction and cerebral infarction, the ginkgo leaf extract and the spinosa seed extract in step S1 are extracted by ultrasound-assisted enzyme to obtain the Chinese medicine extract, and the remaining steps and raw materials are the same as those in Example 3; The preparation method of the Chinese medicine extract comprises the following steps: According to the mass ratio of pretreated ginkgo leaf powder, pretreated spiny jujube kernel powder and polyethylene glycol aqueous solution being 3.9:1.3:160, the pretreated ginkgo leaf powder and pretreated spiny jujube kernel powder are immersed in a 15wt% polyethylene glycol aqueous solution, and then a composite enzyme (the mass of the composite enzyme is 0.6% of the mass of the polyethylene glycol aqueous solution) is added, and extraction is performed by ultrasonic treatment (ultrasonic power is 100W, ultrasonic frequency is 40kHz), the ultrasonic treatment temperature is 60°C, the ultrasonic treatment time is 30min, and after filtration, an extract is obtained, and it is concentrated to one third of the initial volume by reduced pressure distillation at 50°C. Anhydrous ethanol was then added to precipitate the concentrate (the mass of anhydrous ethanol was 30% of the mass of the polyethylene glycol aqueous solution), the precipitate was centrifuged at 4500 rpm for 15 min, washed 3 times with anhydrous ethanol (the mass of anhydrous ethanol each time was 10% of the mass of the polyethylene glycol aqueous solution), and freeze-dried at minus 10°C to obtain the Chinese medicine extract, wherein the ginkgo leaf powder was obtained by drying the ginkgo leaf at 50°C to constant weight, then grinding it, and passing it through an 80-mesh sieve; the Chinese jujube seed powder was obtained by grinding the Chinese jujube seed, and then passing it through a 60-mesh sieve; the complex enzyme was obtained by mixing cellulase, pectinase, and trypsin in a mass ratio of 3:2:1.

[0039] Comparative Example 2 The difference between this comparative example and Example 3 is that when preparing the medicine-food composition with the efficacy of preventing myocardial infarction and cerebral infarction, the ginkgo leaf extract and the spina jujuba seed extract in step S1 are extracted by ultrasound-assisted ethanol to obtain the Chinese medicine extract, and the remaining steps and raw materials are the same as those in Example 3; The preparation method of the Chinese medicine extract comprises the following steps: According to the mass ratio of pretreated ginkgo leaf extract, pretreated spinach seed powder and ethanol aqueous solution of 0.9:0.3:20, the pretreated ginkgo leaf extract and pretreated spinach seed powder were added to 70wt% ethanol aqueous solution, and extracted by ultrasonic treatment (ultrasonic power of 300W, ultrasonic frequency of 40kHz), ultrasonic temperature of 70℃, ultrasonic time of 60min, after filtration, the obtained filtrate was concentrated by vacuum distillation at 55℃, then added to ethanol for precipitation (the mass of ethanol is 50% of the mass of ethanol aqueous solution), washed with deionized water for 3 times (the mass of deionized water each time is equal to the mass of ethanol aqueous solution), freeze-dried at minus 10℃ to obtain spinach seed extract, wherein the ginkgo leaf powder is obtained by drying the ginkgo leaf at 50℃ to constant weight, then grinding and passing through an 80-mesh sieve; the spinach seed powder is obtained by grinding the spinach seed and then passing through a 60-mesh sieve.

[0040] Comparative Example 3 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S2, the probiotics are obtained by uniformly mixing Bifidobacterium longum and Lactobacillus acidophilus at a live bacteria ratio of 2:4, and the remaining steps and raw materials are synchronized with Example 3; S2: Streak the probiotics on MRS solid culture medium, then culture at 38°C for 48 hours to obtain a single colony, then pick the single colony and inoculate it into MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 38°C for 24 hours until colonies grow to obtain activated probiotics, wherein the probiotics are obtained by evenly mixing Bifidobacterium longum and Lactobacillus acidophilus at a live bacteria ratio of 2:4.

[0041] Comparative Example 4 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S2, the probiotics are obtained by uniformly mixing Bifidobacterium longum and Akkermansia muciniphila at a live bacteria ratio of 3:3, and the remaining steps and raw materials are synchronized with Example 3; S2: Streak the probiotics on MRS solid culture medium, and then culture at 38°C for 48 hours to obtain a single colony, and then pick the single colony and inoculate it into MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 38°C for 24 hours until colonies grow to obtain activated probiotics, wherein the probiotics are obtained by evenly mixing Bifidobacterium longum and Akkermansia muciniphila in a live bacteria ratio of 3:3.

[0042] Comparative Example 5 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S2, the probiotics are obtained by uniformly mixing Lactobacillus acidophilus and Akkermansia muciniphila at a live bacteria ratio of 4:2, and the remaining steps and raw materials are synchronized with Example 3; S2: Streak the probiotics on MRS solid culture medium, and then culture at 38°C for 48 hours to obtain a single colony, and then pick the single colony and inoculate it into MRS liquid culture medium. The inoculation amount of the single colony is 5%, and culture it anaerobically at 38°C for 24 hours until colonies grow to obtain activated probiotics, wherein the probiotics are obtained by evenly mixing Lactobacillus acidophilus and Akkermansia muciniphila in a live bacteria ratio of 4:2.

[0043] Comparative Example 6 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the mass of prebiotics is replaced by β-glucan, and the remaining steps and raw materials are synchronized with Example 3; According to the mass ratio of Chinese herbal medicine extract, β-glucan and deionized water of 1.2:1:110, the Chinese herbal medicine extract and β-glucan in step S1 are added to deionized water and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9 CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the stabilizer is composed of L-ascorbic acid, vitamin D2 and plant sterol esters mixed in a mass ratio of 1.2:0.8:0.4.

[0044] Comparative Example 7 The difference between this comparative example and Example 3 is that when preparing the medicinal and edible composition having the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the mass of prebiotics and the like is replaced by Dendrobium officinale polysaccharide, and the remaining steps and raw materials are synchronized with Example 3; S3: According to the mass ratio of Chinese herbal medicine extract, Dendrobium officinale polysaccharide and deionized water of 1.2:1:110, the Chinese herbal medicine extract and Dendrobium officinale polysaccharide in step S1 are added to deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the stabilizer is composed of L-ascorbic acid, vitamin D2 and plant sterol esters mixed in a mass ratio of 1.2:0.8:0.4.

[0045] Comparative Example 8 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the mass of the Chinese medicine extract is replaced by the ginkgo leaf extract, and the remaining steps and raw materials are synchronized with Example 3; S3: According to the mass ratio of ginkgo leaf extract, prebiotics and deionized water of 1.2:1:110, the ginkgo leaf extract and prebiotics in step S1 are added to deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9 CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide mixed in a mass ratio of 0.8:0.5; the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester mixed in a mass ratio of 1.2:0.8:0.4.

[0046] Comparative Example 9 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the mass of the Chinese medicine extract is replaced with the Ziziphus jujuba seed extract, and the remaining steps and raw materials are synchronized with Example 3; S3: According to the mass ratio of the jujube seed extract, prebiotics and deionized water of 1.2:1:110, the jujube seed extract and prebiotics in step S1 are added to the deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide mixed in a mass ratio of 0.8:0.5; the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester mixed in a mass ratio of 1.2:0.8:0.4.

[0047] Comparative Example 10 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition with the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the Chinese medicine extract and the activated probiotics are fermented and then mixed with the prebiotics and the stabilizer, and the other steps and raw materials are the same as those in Example 3; S3: According to the mass ratio of Chinese herbal medicine extract to deionized water of 1.2:110, the Chinese herbal medicine extract in step S1 is added to deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×10 9 CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid, stabilizer and prebiotics of 100:7:0.5, the fermentation liquid is mixed with the stabilizer and prebiotics, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the stabilizer is composed of L-ascorbic acid, vitamin D2 and phytosterol ester in a mass ratio of 1.2:0.8:0.4, and the prebiotic is composed of β-glucan and Dendrobium officinale polysaccharide in a mass ratio of 0.8:0.5.

[0048] Comparative Example 11 The difference between this comparative example and Example 3 is that when preparing the medicine-food homologous composition having the efficacy of preventing myocardial infarction and cerebral infarction, in step S3, the stabilizer is composed of L-ascorbic acid and vitamin D2 mixed in a mass ratio of 1.2:1.2, and the other steps and raw materials are synchronized with Example 3; S3: According to the mass ratio of Chinese herbal medicine extract, prebiotics and deionized water of 1.2:0.5:110, the Chinese herbal medicine extract and prebiotics in step S1 are added to deionized water, and stirred evenly, and then sterilized at 60°C for 20 minutes to obtain a fermentation matrix, and the activated probiotics in step S2 are inoculated into the fermentation matrix, and the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 3×109 CFU / mL, after fermentation treatment, the fermentation temperature is 38°C, the fermentation time is 45h, and the fermentation liquid is obtained. According to the mass ratio of fermentation liquid to stabilizer of 100:7, the fermentation liquid and the stabilizer are mixed, and stirred for 30min, and microfiltered through a 0.22μm polyvinylidene fluoride membrane, and freeze-dried at minus 20°C for 36h to obtain a medicine and food composition with the effect of preventing myocardial infarction and cerebral infarction, wherein the prebiotics are composed of β-glucan and Dendrobium officinale polysaccharide mixed in a mass ratio of 0.8:0.5; the stabilizer is composed of L-ascorbic acid and vitamin D2 mixed in a mass ratio of 1.2:1.2.

[0049] The medicinal and edible compositions prepared in Examples 1-3 and Comparative Examples 1-11 and having the efficacy of preventing myocardial infarction and cerebral infarction were tested for their effects on rat atherosclerosis. The specific method is as follows: After 1 week of adaptive feeding, SPF SD rats were randomly divided into a blank control group, a model group, an embodiment and a comparative example group, and a Chinese medicine extract group, with 10 rats in each group. The model group, the embodiment and the comparative example group, and the Chinese medicine extract group were fed with a high-fat diet combined with an intraperitoneal injection of vitamin D3 to establish an atherosclerosis rat model. The formula of the high-fat diet was as follows: 81.3% basic feed, 10% lard, 5% sucrose, 1% methionine, 0.2% propylthiouracil, and 0.5% sodium cholate.

[0050] The model group, the example group, the comparative example group, and the Chinese medicine extract group were fed with a high-fat diet, and vitamin D3 (60×10 4 IU / kg); the blank control group was fed with basic feed, and an equal amount of normal saline was injected intraperitoneally on the first day of modeling, and the modeling time was 30 days. After modeling, the Example and Comparative Example groups: rats were gavaged with the powder of the medicinal and edible composition with the effect of preventing myocardial infarction and cerebral infarction prepared in Examples 1-3 and Comparative Example 1-11, respectively, and the suspension was prepared, and the gavage dose was 20 mg / kg, and the gavage was continued for 8 nights (blank control group, model group: rats were gavaged with the same dose of normal saline, respectively, and the gavage was continued for 8 nights; Chinese medicine extract group: rats were gavaged with a suspension prepared by mixing ginkgo leaf extract and spiny jujube seed extract, and the gavage dose was 20 mg / kg, and the gavage was continued for 8 nights), and fasted for 12 hours after the last gavage.

[0051] Rats in each group were anesthetized with isoflurane continuously. After laparotomy, blood was collected from the abdominal aorta and centrifuged. The supernatant was tested according to the instructions of MDA, SOD kit (Nanjing Jiancheng Bioengineering Institute), and homocysteine ​​(HCY) detection kit (Shenzhen Tailede Medical). After blood collection, the rats were quickly killed and dissected, and the arteries were removed and fixed with 10% formaldehyde solution. After fixation for 18 hours, the tissue samples were made into 4mm paraffin sections and stained with hematoxylin-eosin (HE). Finally, the sections were sealed with neutral gum, and PHOTOSHOP (software) was used to calculate the area of ​​atherosclerotic plaques.

[0052] Compared with the blank control group, SOD in the model group was significantly decreased, MDA was significantly increased, and HCY was significantly increased, with significant statistical differences. At the same time, the rats in the model group showed typical symptoms of atherosclerosis in the vascular wall, indicating that the rat atherosclerosis model was successfully established.

[0053] Compared with the model group, the abnormal SOD and MDA levels in the embodiment and comparative groups and the Chinese medicine extract group were restored. In particular, the embodiment and comparative groups to which rats were gavaged with a suspension prepared from the powder of the medicine-food composition with the efficacy of preventing myocardial infarction and cerebral infarction prepared in Examples 1-3 showed significant antioxidant and atherosclerosis-improving effects (P<0.05 or P<0.01).

[0054] The test results are shown in Table 1 below: Table 1 Effects of the medicinal and edible compositions prepared in Examples 1-3 and Comparative Examples 1-11 on atherosclerosis in rats )

[0055]

[0056] Note: Compared with the blank control group, <0.05, <0.01; compared with the model group, <0.05, <0.01.

[0057] It can be seen from the data in Table 1 above that, by comparing Comparative Examples 1-2 with Example 3, the ginkgo leaf extract and the spinach seed extract in step S1 are extracted by ultrasound-assisted enzyme, or the ginkgo leaf extract and the spinach seed extract in step S1 are extracted by ultrasound-assisted ethanol, and finally a medicine-food composition with the effect of preventing myocardial infarction and cerebral infarction is prepared. The test results are worse than those in Example 3, indicating that the extraction of ginkgo leaf extract by ultrasound-assisted enzyme and the extraction of spinach seed extract by ultrasound-assisted ethanol have better extraction efficiency and yield, show stronger antioxidant activity, can further improve atherosclerosis, and have a better effect of preventing myocardial infarction and cerebral infarction. By comparing Comparative Examples 3-5 with Example 3, it can be seen that the probiotics are obtained by uniformly mixing Bifidobacterium longum and Lactobacillus acidophilus, or the probiotics are obtained by uniformly mixing Bifidobacterium longum and Akkermansia muciniphila, or the probiotics are obtained by uniformly mixing Lactobacillus acidophilus and Akkermansia muciniphila, and finally a medicine-food homologous composition with the effect of preventing myocardial infarction and cerebral infarction is prepared. The test results are worse than those of Example 3, indicating that the probiotics are obtained by uniformly mixing Bifidobacterium longum, Lactobacillus acidophilus, and Akkermansia muciniphila according to a certain live bacteria count ratio, and the three bacteria have a good synergistic effect, which can effectively regulate the balance of intestinal flora, inhibit the growth and reproduction of harmful bacteria, and have a good preventive effect on atherosclerosis; By comparing Comparative Examples 6-11 with Example 3, it can be seen that the mass of prebiotics is replaced by β-glucan or Dendrobium officinale polysaccharide, or the mass of Chinese medicine extract is replaced by Ginkgo biloba extract or Ziziphus jujuba seed extract; or the Chinese medicine extract and activated probiotics are fermented and then mixed with prebiotics and stabilizers, or the stabilizer is composed of L-ascorbic acid and vitamin D2, and finally a medicine-food homologous composition with the effect of preventing myocardial infarction and cerebral infarction is prepared. The test results are worse than those of Example 3, indicating that the prebiotic composed of β-glucan and Dendrobium officinale polysaccharide can reduce Cholesterol, improve atherosclerosis, and provide nutrients for probiotics; Ginkgo leaf extract and Ziziphus jujuba seed extract are mixed to form a traditional Chinese medicine extract, the two have a good synergistic effect, strong antioxidant property, and can effectively improve atherosclerosis; After the traditional Chinese medicine extract, prebiotics, and activated probiotics are fermented, a stabilizer is added to make the prepared pharmaceutical composition have a good effect of preventing myocardial infarction and cerebral infarction; The stabilizer composed of a mixture of L-ascorbic acid, vitamin D2, and plant sterol esters can further improve the stability of the pharmaceutical composition and enable it to exert a better efficacy.

[0058] It can be seen from the data in Table 1 above that the edible-medicinal composition with the efficacy of preventing myocardial infarction and cerebral infarction prepared in Example 1-3 is compared with the edible-medicinal composition with the efficacy of preventing myocardial infarction and cerebral infarction prepared in Comparative Examples 1-11. The Chinese medicine extract is obtained by mixing the ginkgo leaf extract and the jujube seed extract; the activated probiotics are obtained after the probiotics are activated; the Chinese medicine extract, the prebiotics and the activated probiotics are fermented and then mixed with a stabilizer to finally prepare the edible-medicinal composition with the efficacy of preventing myocardial infarction and cerebral infarction, which meets the test performance requirements, while the edible-medicinal composition with the efficacy of preventing myocardial infarction and cerebral infarction prepared in Comparative Examples 1-11 does not meet the performance requirements. This shows that the edible-medicinal composition with the efficacy of preventing myocardial infarction and cerebral infarction prepared by the present invention not only has a higher bioavailability and better stability, but also has less toxic and side effects, and has a better preventive effect in preventing myocardial infarction and cerebral infarction.

[0059] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0060] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction, characterized in that: The following steps are involved: S1: Evenly mix the ginkgo leaf extract and the spinosa jujube seed extract to obtain a Chinese medicine extract; S2: activating the probiotics to obtain activated probiotics; S3: The Chinese herbal extract, prebiotics and activated probiotics in step S1 are fermented and then mixed with a stabilizer, and the mixture is filtered and freeze-dried to obtain a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction.

2. The method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction according to claim 1, characterized in that: In step S1, the method for preparing the ginkgo leaf extract comprises the following steps: The pretreated ginkgo leaf powder is immersed in a polyethylene glycol aqueous solution, and then a complex enzyme is added and extracted by ultrasonic treatment. After filtration, an extract is obtained, and the extract is concentrated by reduced pressure distillation at 40-50°C, and then anhydrous ethanol is added to precipitate the concentrate. The obtained precipitate is centrifuged for 15-25 minutes, washed with anhydrous ethanol, and freeze-dried to obtain a ginkgo leaf extract.

3. The method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction according to claim 1, characterized in that: In step S1, the method for preparing the Chinese date seed extract comprises the following steps: The pretreated spiny jujube seed powder is added to an ethanol aqueous solution and extracted by ultrasonic treatment. After filtering, the obtained filtrate is concentrated by vacuum distillation at 45-55° C., then added to ethanol for precipitation, washed with deionized water, and freeze-dried to obtain the spiny jujube seed extract.

4. The method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction according to claim 1, characterized in that: Step S2 is specifically as follows: The probiotics were streaked onto MRS solid culture medium, and then cultured at 36-38°C for 36-48 hours to obtain single colonies, and then single colonies were picked and inoculated into MRS liquid culture medium, and anaerobically cultured at 35-38°C for 18-24 hours until colonies grew to obtain activated probiotics.

5. The method for preparing a medicine-food composition having the effect of preventing myocardial infarction and cerebral infarction according to claim 4, characterized in that: The probiotics are obtained by uniformly mixing Bifidobacterium longum, Lactobacillus acidophilus and Akkermansia muciniphila according to a live bacteria count ratio of 1:2-4:

1.

6. The method for preparing a medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction according to claim 1, characterized in that: Step S3 is specifically as follows: The Chinese herbal extract and prebiotics in step S1 are added to deionized water, stirred evenly, and then sterilized for 15-20 minutes to obtain a fermentation matrix, the activated probiotics in step S2 are inoculated into the fermentation matrix, and fermentation treatment is performed to obtain a fermentation liquid, the fermentation liquid is mixed with a stabilizer, stirred for 20-30 minutes, microfiltered through a filter membrane, and freeze-dried for 24-36 hours to obtain a medicine-food composition with the effect of preventing myocardial infarction and cerebral infarction.

7. The method for preparing a medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction according to claim 6, characterized in that: The prebiotics are composed of beta-glucan and dendrobium officinale polysaccharide mixed in a mass ratio of 0.7-0.8:0.4-0.

5.

8. The method for preparing a medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction according to claim 6, characterized in that: In step S3, the amount of activated probiotics added to the fermentation matrix is ​​controlled to be 2×10 9 -3×10 9 CFU / mL.

9. A medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction obtained by the preparation method according to any one of claims 1 to 8.

10. Use of the medicine-food composition having the efficacy of preventing myocardial infarction and cerebral infarction as claimed in claim 9 in the preparation of health products, functional foods or functional drinks.

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