Ganoderma lucidum spore fermentation product, preparation method and application thereof in cosmetics

Abstract

The present invention provides a Ganoderma lucidum spore fermentation product, a preparation method and its application in cosmetics, which belongs to the field of microbial fermentation technology. A Ganoderma lucidum spore fermentation product is prepared by fermenting Torulopsis apis, wherein Torulopsis apis is Torulopsis apis. Torulopsis apicola CICC 1706, the fermentation medium contains Ganoderma lucidum spore powder and glucose, and the Ganoderma lucidum spore fermentation product contains triterpenes and sophorolipids. The present invention promotes the slow release of Ganoderma lucidum spore oil during the fermentation process through the metabolism of Torulopsis mellifera. Simultaneously, some of the oil reacts with the sugar in the medium to produce sophorolipids, resulting in the fermentation product forming an emulsion-like system, thereby achieving efficient extraction of Ganoderma lucidum spore oil.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial fermentation, and in particular relates to a ganoderma lucidum spore fermentation product, a preparation method and application thereof in cosmetics. Background Art

[0002] Ganoderma lucidum spore oil is an oily liquid extracted from Ganoderma lucidum spores by breaking their spore walls. It contains fatty acids, sterols, terpenes, and other ingredients. The active ingredients, particularly the Ganoderma triterpenes, possess anti-aging, anti-tumor, and immune-enhancing properties, and are widely used in health supplements. In recent years, the application of Ganoderma lucidum spore oil in cosmetics and skincare has gradually gained recognition among consumers for its anti-aging and skin-repair benefits, demonstrating its significant skincare value.

[0003] There are two main methods for extracting Ganoderma lucidum spore oil: organic solvent extraction and supercritical carbon dioxide extraction. Organic solvent extraction technology is relatively mature, but it usually requires the use of organic solvents such as volatile alcohols and petroleum ether, which has problems such as high energy consumption, high solvent usage, solvent residue, and safe production. In addition, traditional organic solvent extraction methods may damage the heat-sensitive components in Ganoderma lucidum spore oil, affecting its activity. Supercritical carbon dioxide extraction has gradually attracted attention due to its advantages such as environmental protection, low energy consumption, and low-temperature extraction. This method can be carried out at a lower temperature, avoiding the degradation of heat-sensitive components, and at the same time, no organic solvent is used, avoiding the problem of solvent residue. However, supercritical carbon dioxide extraction equipment is expensive, and in actual application, a small amount of organic solvent still needs to be added as an "entrainer" to enhance the extraction efficiency, which may lead to the risk of solvent residue, for example, the methods disclosed in patent documents CN104073344B or CN103301174B.

[0004] Water is the primary solvent for many cosmetics today. If oil-based ingredients are used, surfactants are often added to aid solubility and emulsification. However, the vast majority of surfactants used in cosmetics are chemically synthesized or semi-synthetic. Furthermore, most cosmetics manufacturers have high requirements for raw material stability.

[0005] In view of the above problems, it is particularly urgent to develop a raw material containing Ganoderma lucidum spore oil that is more suitable for use in cosmetics. Summary of the Invention

[0006] In view of this, the technical problem to be solved by the present invention is to provide a preparation method of Ganoderma lucidum spore fermentation product, which promotes the slow release of Ganoderma lucidum spore oil during the fermentation process through the metabolic action of Torulopsis mellifera; at the same time, Torulopsis mellifera utilizes part of the oil in Ganoderma lucidum spores and the sugar in the culture medium to synthesize sophorolipids, so that the fermentation product forms an emulsion-like system, thereby realizing the efficient extraction of Ganoderma lucidum spore oil.

[0007] In one aspect, the present invention provides a Ganoderma lucidum spore fermentation product, which is prepared by fermenting Torulopsis apis, wherein the Torulopsis apis is Torulopsis apis. Torulopsis apicola CICC 1706, the fermentation medium contains Ganoderma lucidum spore powder and glucose, and the Ganoderma lucidum spore fermentation product contains triterpenes and sophorolipids.

[0008] In another aspect, the present invention provides a method for preparing a Ganoderma lucidum spore fermentation product, comprising:

[0009] Torulopsis apis Torulopsis apicola CICC 1706 is a strain,

[0010] The fermentation is carried out in a fermentation medium containing ganoderma lucidum spore powder and glucose to obtain a ganoderma lucidum spore fermentation product.

[0011] Optionally, the fermentation medium contains 125-140 g / L broken wall Ganoderma lucidum spore powder, 40-45 g / L glucose, 2.5 g / L yeast extract powder, 4 g / L corn steep liquor, natural pH, and is sterilized at 105° C. for 20 min.

[0012] Optionally, the fermentation process parameters include: inoculating the seed liquid of the honeybee yeast into the culture medium, fermenting and culturing at 32-33°C for 60-72 hours, then reducing the temperature to 27°C and continuing to ferment for 24-30 hours, stirring at a speed of 400-480 rpm, controlling the dissolved oxygen content to be greater than 40%, and obtaining a fermentation liquid.

[0013] The research of the present invention shows that sophorolipids are more produced in the stable period (late stage) of fermentation. Properly lowering the temperature can reduce the reproduction of the bacteria, prolong the stable period, and is conducive to moderately increasing the production of sophorolipids, thereby meeting the application requirements of the Ganoderma lucidum spore fermentation product of the present invention.

[0014] Optionally, the Torulopsis apis seed liquid is prepared by the following method: activating the Torulopsis apis strain, inoculating it into a seed liquid culture medium, and culturing it aerobically at 33-34° C. for 32-36 hours to obtain the seed liquid.

[0015] Optionally, the seed liquid culture medium contains 10 g / L yeast extract, 20 g / L peptone, 50 g / L glucose, 6 g / L olive oil, has a natural pH, and is sterilized at 115° C. for 15 min.

[0016] Optionally, the method for preparing a Ganoderma lucidum spore fermentation product further includes: a post-processing step, wherein the post-processing step includes: heating the fermentation liquid to 60° C. and maintaining it for 40 minutes to inactivate bacteria, stopping fermentation, and sequentially centrifuging, filtering with filter cloth, and microfiltering to obtain the Ganoderma lucidum spore fermentation product.

[0017] Optionally, the filter cloth filtration uses an 800-mesh filter cloth, and the microfiltration uses a 0.1 or 0.22 μm filter.

[0018] On the other hand, the present invention also provides the use of the Ganoderma lucidum spore fermentation product in cosmetics.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention screens and uses Torulopsis apis Torulopsis apicola CICC 1706, this strain adapts to special fermentation needs, can efficiently metabolize the oil in Ganoderma lucidum spores, and can produce a certain amount of sophorolipids to improve the stability of the fermentation liquid.

[0021] The fermentation process of the present invention adopts a completely organic culture medium instead of commonly used inorganic salts, which is more in line with the use scenarios and consumption concepts of cosmetics.

[0022] The present invention ferments Ganoderma lucidum spore powder with Tocopherols, a yeast from the honey bee, to promote the slow release of Ganoderma lucidum spore oil during the fermentation process. Simultaneously, some oil reacts with sugars in the culture medium to produce glycosophorolide, which acts as a surfactant and slowly forms an emulsified system with other oily components. This eliminates the need for additional surfactants for solubilization and emulsification, thereby achieving efficient extraction of Ganoderma lucidum spore oil and providing a new technical path for the green and efficient preparation of Ganoderma lucidum spore oil. The Ganoderma lucidum spore fermentation product of the present invention has superior antioxidant, anti-aging, and skin-repairing effects, and has good application prospects in cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 : Cell migration assay results. DETAILED DESCRIPTION

[0025] In order to better understand the present invention, the content of the present invention is further clearly set forth below in conjunction with the examples, but the protection content of the present invention is not limited to the following examples. In the following description, a large number of specific details are provided in order to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details.

[0026] The honey bee yeast of the present invention Torulopsis apicolaCICC 1706, Candida albicans Candida bombicola ATCC 22214, the depository is China Industrial Microbiological Culture Collection Center; the brewer's yeast Saccharomyces cerevisiae CCTCC M2016509, deposited by China Center for Type Culture Collection. The above strains were purchased as freeze-dried powder.

[0027] The ganoderma lucidum spore powder of the present invention is passed through a 100-mesh sieve and is purchased.

[0028] Example 1: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps:

[0029] Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 34°C and 220 rpm for 32 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, pH natural, sterilized at 115℃ for 15min;

[0030] 100 mL of seed liquid was inoculated into 4 L of fermentation medium, and the culture temperature was first 32 ° C for 72 h, then the temperature was lowered to 27 ° C and the fermentation temperature was continued for 30 h. The stirring speed was 450 rpm and the dissolved oxygen content was controlled to be > 40%. -1 ): 130g Ganoderma lucidum spore powder, 40g glucose, 2.5g yeast extract powder, 4g corn steep liquor, pH natural, sterilized at 105℃ for 20min;

[0031] The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered through an 800-mesh filter cloth and a 0.1 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0032] Example 2: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps:

[0033] Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 34°C and 220 rpm for 36 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, pH natural, sterilized at 115℃ for 15min;

[0034] 600 mL of seed liquid was inoculated into 30 L of fermentation medium, and the temperature was first 33 ° C for 63 h, then the temperature was lowered to 27 ° C for 24 h, the stirring speed was 400 rpm, and the dissolved oxygen content was controlled to be > 40%; wherein: the fermentation medium (L -1 ): 140g broken wall Ganoderma lucidum spore powder, 45g glucose, 2.5g yeast extract powder, 4g corn steep liquor, pH natural, sterilized at 105℃ for 20min;

[0035] The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered through an 800-mesh filter cloth and a 0.22 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0036] Example 3: A method for preparing a Ganoderma lucidum spore fermentation product, comprising the following steps:

[0037] Torulopsis apis Torulopsis apicola After the CICC 1706 strain was activated on the plate, it was inoculated into the seed liquid culture medium and cultured at 33°C and 220 rpm for 35 h to obtain the seed liquid; wherein: the seed liquid culture medium (L -1 ): 10g yeast extract, 20g peptone, 50g glucose, 6g olive oil, pH natural, sterilized at 115℃ for 15min;

[0038] 115 mL of seed liquid was inoculated into 5 L of fermentation medium, and the culture temperature was 32.5 ° C for 60 h, then the temperature was lowered to 27 ° C and the fermentation temperature was continued for 27 h. The stirring speed was 480 rpm and the dissolved oxygen content was controlled to be greater than 40%. -1 ): 125g Ganoderma lucidum spore powder, 42g glucose, 2.5g yeast extract powder, 4g corn steep liquor, pH natural, sterilized at 105℃ for 20min;

[0039] The temperature was raised to 60°C and maintained for 40 minutes to inactivate the bacteria, and the fermentation was stopped. The fermentation liquid was first centrifuged to remove most of the solids, and then filtered through an 800-mesh filter cloth and a 0.22 μm filter to obtain the Ganoderma lucidum spore fermentation product.

[0040] Control Example 1: Control Example 1 provides a Ganoderma lucidum spore oil and spore oil emulsion extracted by an organic solvent, the method is as follows: 130g of Ganoderma lucidum spore powder is added with 1000mL of n-hexane, ultrasonicated at room temperature (25°C) for 30min, shaken, allowed to stand, filtered to obtain the filtrate, and evaporated to dryness under reduced pressure to obtain Ganoderma lucidum spore oil.

[0041] Control Example 2: Control Example 2 provides a Ganoderma lucidum spore oil and spore oil emulsion extracted by supercritical CO2 method. The method is as follows: take Ganoderma lucidum spore powder, granulate it, pass it through a 60-mesh sieve, weigh 130g of Ganoderma lucidum spore granules and fill them into a 500mL supercritical CO2 extraction kettle. The extraction conditions are 30MPa, 45℃, and 3h, and the Ganoderma lucidum spore oil is collected.

[0042] Control Example 3: Compared with Example 1, only the strain was replaced by: Candida bumblebee Candida bombicola ATCC22214, and the other process conditions remained unchanged.

[0043] Comparative Example 4: Compared with Example 1, only the strain was replaced by: Saccharomyces cerevisiae Saccharomyces cerevisiae CCTCC M2016509, and the other process conditions remained unchanged.

[0044] Reference Example 5: Reference Example 5 provides a method for extracting spore powder using a biosurfactant sophorolipid to obtain a spore oil solution, comprising:

[0045] S1. Dissolve 40 g of sophorolipid in 1960 g of pure water as an extract;

[0046] S2. Take 260g of broken-wall Ganoderma lucidum spore powder and mix it with the above extract;

[0047] S3, heating and stirring, raising the temperature to 80°C and maintaining for 3 hours;

[0048] S4, stop heating, centrifuge the mixture, and take the supernatant;

[0049] S5. The supernatant was filtered using qualitative filter paper and 0.22 μm filter membrane in turn.

[0050] The following tests and analyses were performed on the above Examples 1-2 and Comparative Examples 1-5.

[0051] 1. Physical and chemical performance testing

[0052] The solid residues obtained by centrifugation after fermentation in Examples 1 and 2 and Control Examples 3 and 4 (only the dark brown portion with uniform color was collected), and the solids obtained by centrifugation of the extract in Control Example 5 were collected, dried at 65°C, and stored for the detection of the oil content of the residue.

[0053] The products of Examples 1 and 2, and Reference Examples 3 and 5 were taken, and the pH was adjusted to less than 2 using hydrochloric acid. The samples were frozen and then thawed, allowed to stand for 24 hours, and the solution was separated into layers. The oily substance in the upper layer was collected for the detection of triterpene content and peroxide value. The product of Reference Example 4 contained too little oil, so the collection of the triterpene content and peroxide value could not be completed.

[0054] 1.1. Residue oil content detection

[0055] The oil content of the residue after extraction and fermentation of Ganoderma lucidum spore powder was determined with reference to the test method in GB / T 14488.1-2008, Oil Content of Vegetable Oils. The test results are shown in Table 1. The lower the oil content in the residue, the more efficient the extraction or fermentation method is, and the more effective it is in extracting Ganoderma lucidum spore oil.

[0056] Table 1 Residue oil content test results

[0057]

[0058] The results in Table 1 show that the oil content in the residue obtained by the fermentation methods of Examples 1 and 2 of the present invention is less than 4%. Compared with Control Examples 3 and 4, the fermentation method of the present invention is significantly more efficient in extracting Ganoderma lucidum spore oil from Ganoderma lucidum spore powder and can more fully extract Ganoderma lucidum spore oil.

[0059] 1.2. Triterpene content and peroxide value detection

[0060] The vanillin-perchloric acid method was used to determine the triterpenoid content of the oily component according to the method described in the reference (Wang Hongxia, Zhao Zhenyu, Dong Xiaofen, et al. Study on the preparation process of Ganoderma lucidum spore oil [J]. China Food Additives, 2024, 35(1): 224-230). The peroxide value was determined according to GB 5009.227-2016 National Food Safety Standard for the Determination of Peroxide Value in Foods. The test results are shown in Table 2.

[0061] Table 2 Triterpenoid content and peroxide value test results

[0062]

[0063] The results in Table 2 show that the content of oily triterpenes in the products of Examples 1 and 2 of the present invention is significantly higher than that of the control example. This is because part of the fat in the Ganoderma lucidum spore oil in Examples 1 and 2 is converted into sophorolipids by the fermentation bacteria, resulting in a relatively increased triterpene content. At the same time, the peroxide values of the products of Examples 1 and 2 of the present invention are lower, indicating that the products obtained by the method of the present invention are more stable during storage and use.

[0064] 1.3. Sophorolipid content detection

[0065] The sophorolipid content in the fermentation products of Examples 1 and 2 and Comparative Examples 3 and 4 was determined using the method described in the reference (Shen Jing. Research on the synthesis of sophorolipids by Pseudomonas aeruginosa using medium-chain-length alkanes [D]. Shandong University, 2012). The sophorolipid content in the fermentation products of Examples 1 and 2 and Comparative Examples 3 and 4 was determined by first extracting the fermentation products of Ganoderma lucidum spores with ethyl acetate and then detecting and converting them using the anthrone method. The test results are shown in Table 3.

[0066] Table 3 Sophorolipid content detection results

[0067]

[0068] The results in Table 3 show that the fermentation products of Examples 1 and 2 of the present invention contain significantly more sophorolipids, while the fermentation products of Control Examples 3 and 4 contain relatively lower sophorolipid contents, especially Control Example 4, which can be considered to produce no sophorolipids. This result also explains the inherent reason why Examples 1 and 2 of the present invention can efficiently transfer oil from Ganoderma lucidum spore powder.

[0069] 2. Efficacy testing

[0070] Since the products obtained in Examples 1 and 2 are pure oily components, in order to facilitate the comparison of Examples 1 and 2 with other control examples and to conduct cell experiments, the products of Control Examples 1 and 2 were respectively processed as follows. The specific steps are as follows:

[0071] S1. Dissolve 0.1g xanthan gum in 94.5g pure water, stir to dissolve and slowly heat to 85℃;

[0072] S2. Take 3.4 g of the product of Control Example 1 or 2, mix with 2 g of sophorolipid, and heat to 85°C;

[0073] The mixture of steps S3 and S2 is added to the solution of step S1 and homogenized by a homogenizer until an emulsion is formed.

[0074] 2.1 Free radical scavenging test

[0075] The test method followed "T / SHRH 006-2018 Cosmetics - Free Radical (DPPH) Scavenging Test Method." The DPPH free radical scavenging rate was measured by diluting the samples with pure water to concentrations of 0.5%, 1%, 2%, and 4%. Control Examples 1 and 2 were emulsified according to the aforementioned process, forming emulsions that were then diluted. The test results are shown in Table 4.

[0076] Table 4 Free radical scavenging rate test results

[0077]

[0078] The results in Table 4 show that at the same dilution concentration, the free radical scavenging rates of the products of Examples 1 and 2 of the present invention are more significant, indicating that the antioxidant properties of the products of Examples 1 and 2 of the present invention are more excellent.

[0079] 2.2 Cell migration assay

[0080] The test method was based on the method described in the literature (Liang CC, Park AY, Guan J L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro [J]. Nature Protocols, 2007, 2 (2): 329-333.) to detect the cell scratch repair rate after 24 hours. In control examples 1 and 2, sophorabiosin was emulsified to form an emulsion and then diluted to a concentration of 0.5%. At this concentration, all test samples showed no cytotoxicity. The blank control group was normal saline. The test results are as follows: Figure 1 shown.

[0081] Figure 1 The results showed that the products of Examples 1 and 2 of the present invention could more significantly improve the cell migration rate, indicating that the products of Examples 1 and 2 have the potential to promote tissue repair and regeneration.

[0082] 3. Application testing

[0083] Application Example: The fermentation product of Example 1 is applied to a cosmetic to prepare a Ganoderma lucidum fermented anti-wrinkle moisturizing lotion. The formulation is as follows:

[0084] Table 5 Cosmetic formulation containing the fermentation product of Example 1

[0085]

[0086] Twelve healthy female volunteers aged 33 to 54 years with dry skin and fine lines around the eyes were recruited. Samples were prepared according to the above formula, placed in pump bottles, and given to volunteers for use. The volunteers were instructed to use the product three times daily, morning, noon, and evening, dispensing two pumps and applying them evenly to the face for 14 consecutive days. Relevant tests and evaluations were performed before and 14 days after use. Transepidermal water loss (TEWL) and skin elasticity (R2) were measured using the Courage+Khazaka Multi-Probe Skin Test System (MPA6). A reduced TEWL value indicates improved skin barrier function (repair efficacy), while a high R2 index indicates good skin elasticity. The results are shown in Table 6.

[0087] Table 6 Human test results

[0088]

[0089] The results in Table 6 show that application of the Ganoderma lucidum spore fermentation product of the present invention significantly reduced transepidermal water loss (TEWL) in the skin, indicating a significant improvement in skin barrier function and the excellent repair efficacy of the Ganoderma lucidum spore fermentation product. Simultaneously, the skin elasticity R2 index increased, demonstrating significant improvement in skin elasticity, further demonstrating that the fermentation product of the present invention significantly enhances skin elasticity.

[0090] In summary, the present invention ferments Ganoderma lucidum spore powder by Tocopherols apis, thereby promoting the slow release of Ganoderma lucidum spore oil during the fermentation process; Tocopherols apis utilizes the oil in part of the Ganoderma lucidum spores and the sugar in the culture medium to synthesize sophorolipids, and the sophorolipids serve as surfactants to slowly form an emulsification system with other oily components, thereby achieving solvent-free extraction of Ganoderma lucidum spore oil. The Ganoderma lucidum spore fermentation product obtained by this preparation method has more excellent antioxidant, anti-aging and repair effects, and has good application prospects in cosmetics.

[0091] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention and are not limiting. Other modifications or equivalent substitutions made to the technical solution of the present invention by ordinary technicians in this field should be included in the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for preparing a Ganoderma lucidum spore fermentation product, characterized in that: Torulopsis apis Torulopsis apicola CICC 1706 is a strain, Fermenting in a fermentation medium containing Ganoderma lucidum spore powder and glucose to obtain a Ganoderma lucidum spore fermentation product, wherein the Ganoderma lucidum spore fermentation product contains triterpenes and sophorolipids, wherein the fermentation medium contains 125-140 g / L of broken-wall Ganoderma lucidum spore powder, 40-45 g / L of glucose, 2.5 g / L of yeast extract powder, 4 g / L of corn steep liquor, a natural pH, and sterilization at 105° C. for 20 min; The fermentation process parameters include: inoculating the seed liquid of the honey bee Torulopsis mellifera into a culture medium, fermenting and culturing at 32-33° C. for 60-72 hours, then reducing the temperature to 27° C. and continuing fermentation for 24-30 hours, stirring at a speed of 400-480 rpm, and controlling the dissolved oxygen content to be greater than 40% to obtain a fermentation liquid. The honey bee Torulopsis mellifera seed liquid is prepared by the following method: activating the honey bee Torulopsis mellifera strain, inoculating it into a seed liquid culture medium, culturing it aerobically at 33-34° C. for 32-36 hours to obtain a seed liquid. The seed liquid culture medium contains 10 g / L yeast extract, 20 g / L peptone, 50 g / L glucose, 6 g / L olive oil, a natural pH, and sterilizing at 115° C. for 15 minutes. The method further comprises a post-processing step, wherein the post-processing step comprises heating the fermentation liquid to 60° C. and maintaining the temperature for 40 minutes to inactivate bacteria, stopping the fermentation, and sequentially centrifuging, filtering with a filter cloth, and microfiltering to obtain a Ganoderma lucidum spore fermentation product.

2. The method for preparing a Ganoderma lucidum spore fermentation product according to claim 1, wherein: The filter cloth filtration adopts 800 mesh filter cloth, and the microfiltration adopts 0.1 or 0.22 μm filter.

3. Use of the Ganoderma lucidum spore fermentation product obtained by the method for preparing a Ganoderma lucidum spore fermentation product according to claim 1 or 2 in the preparation of cosmetics, wherein the cosmetics have antioxidant, anti-aging and repairing effects.

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