Ilex latifolia thunb extract, composition containing same and application of Ilex latifolia thunb extract in preparation of hypolipidemic drugs

Abstract

The invention provides a lobular broadleaf holly leaf extract, a composition containing the lobular broadleaf holly leaf extract and application of the lobular broadleaf holly leaf extract to preparation of blood fat reducing medicine, and relates to the technical field of natural product extracts.The preparation method of the extract comprises the steps that raw material pretreatment is conducted, specifically, lobular broadleaf holly leaves are smashed, sieved and subjected to microwave treatment; carrying out water extraction, alcohol extraction and concentration extraction, and mixing the obtained products to obtain a lobular formosan lattuce herb extract; compared with the prior art, the microwave treatment technology is creatively used, so that the finally extracted component can effectively reduce the blood fat level of a mouse with hyperlipidemia, improve serum indexes, recover the liver function of the mouse and reduce inflammation and oxidative stress levels, and can be used for preparing health care products for improving hyperlipidemia. The broadleaf holly leaf ingredients are efficiently utilized, the ingredient loss is reduced, the yield is effectively improved, the preparation process is optimized, and the method is reliable and simple and has a wide market prospect.

Description

Technical Field

[0001] The invention provides a small-leaf kuding extract, a composition containing the same and use of the extract in preparing a lipid-lowering drug, and relates to the technical field of natural product extracts. Background Art

[0002] Hyperlipidemia, also known as hyperlipidemia, refers to high blood lipid levels. It is a systemic disease in which plasma lipids are higher than normal due to abnormal fat metabolism or operation. Specifically, hyperlipidemia refers to high cholesterol or triglycerides in the blood, or low high-density lipoprotein cholesterol. Modern medicine calls it dyslipidemia. Hyperlipidemia is divided into primary and secondary types. Primary hyperlipidemia is usually related to a variety of factors such as environment and genetics, while secondary hyperlipidemia is often caused by other diseases or drugs. The high-risk population for hyperlipidemia is mainly obese people, the elderly, and other patients with metabolic disorders (such as diabetes, hypertension, myxedema, liver disease, hyperadrenocortical function, etc.). Its typical symptoms include vascular sclerosis, yellow nodules, high blood pressure, etc. In addition, patients may also experience dizziness, unconsciousness, slow thinking, yellow or black patches on the skin, blurred vision, tinnitus, hearing loss, chest tightness and shortness of breath, difficulty breathing, limb fatigue, numbness of hands and feet, etc. Treatments for hyperlipidemia mainly include diet therapy, drug therapy and lifestyle adjustments. Diet therapy recommends consuming low-calorie, low-fat, low-cholesterol, low-sugar, high-fiber foods, such as lean pork, beef, chicken, fish, etc. Drug therapy includes statins (such as simvastatin, atorvastatin) and fibrates (such as fenofibrate, bezafibrate), etc. At the same time, adjusting lifestyle, such as establishing work and rest habits that are in line with the biological clock and participating in appropriate sports (such as jogging, cycling, swimming and other aerobic exercises), can also help improve the condition.

[0003] Small-leaf Kudingcha, also known as Green Mountains and Green Waters, is a non-tea product made from the fresh leaves of the Ligustrum robustum plant of the Oleaceae family. It mainly grows in the jungle deep in the mountains of Yunlian County, Yibin City, Sichuan Province, my country, and is also distributed in Yunnan, Guizhou, Zhejiang and other places. The appearance of small-leaf Kudingcha is green, the soup is green, the bottom of the leaves is bright green, the entrance is slightly bitter and fragrant, and the bitterness is sweet. It is rich in nutrients such as vitamin C, flavonoids, protein and ursolic acid, and the caffeine content is extremely low. Small-leaf Kudingcha is favored by the public because of its bitter taste and cold nature, and has medicinal and health functions such as clearing heat and detoxification, anti-inflammatory and sterilization, anti-oxidation, weight loss and diuresis. It is also loved by consumers for its unique taste and health benefits. It is slightly bitter and slightly sweet when it is first drunk, and then a faint sweetness permeates the mouth. It is a substitute tea drink without caffeine. It is most suitable for drinking in summer, which can clear heat and detoxify, improve eyesight and clear the liver, benefit the throat and moisten the lungs, etc. However, people with weak constitution, pregnant women, women during menstruation and patients with chronic gastroenteritis should not drink it. The research on the application of small leaf Kudingcha extract in hyperlipidemia is not ideal. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a small leaf kuding extract, a composition containing the same and use in the preparation of lipid-lowering drugs, the specific scheme is as follows:

[0005] First, the present invention provides a small leaf kudzu extract, and the preparation method thereof comprises the following steps:

[0006] (1) Raw material pretreatment: weigh high-quality, dry small-leaf Kuding tea leaves, crush and sieve to obtain tea powder; fully mix the obtained tea powder with water and then perform microwave treatment;

[0007] (2) Extraction: the product obtained in step 1 is further added with water and subjected to a first heating extraction, and filtered to obtain filtrate 1 and filter residue 1; the obtained filter residue 1 is added with ethanol aqueous solution and subjected to a second heating extraction, and filtered to obtain filtrate 2; the filtrate 1 and the filtrate 2 are mixed to obtain an extract;

[0008] (3) Concentration: Use a rotary evaporator to reduce the pressure and concentrate the extract until it becomes viscous;

[0009] (4) Organic solvent extraction: extracting and separating the concentrated extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and repeating each step three times to obtain a petroleum ether component, an ethyl acetate component, an n-butanol component, and a water-soluble component in sequence;

[0010] (5) Drying: The extracts of the various components obtained are dried under vacuum to obtain freeze-dried powders of petroleum ether component, ethyl acetate component, n-butanol component, and water-soluble component, which are mixed to obtain the extract of Ilex scoparia.

[0011] Preferably, the sieving in step 1 is through a 50-100 mesh sieve.

[0012] Preferably, in step 1, the mass ratio of the ground tea leaves to water is 1:(2-3), the microwave treatment power is 500-700W, the temperature is controlled at 60-80°C, the total treatment time is 30-60s, and intermittent treatment is adopted, with an interval of 10s after each treatment of 15s before the next treatment.

[0013] Preferably, in step 2, water is continued to be added, wherein the mass ratio of the product of step 1 to water is 1:(10-20), and the first heating and leaching is performed at a temperature of 80-90° C. and for 30-60 min.

[0014] Preferably, in step 2, the ethanol in the ethanol aqueous solution accounts for 70-80 vol%, the mass ratio of the filter residue 1 to the ethanol aqueous solution is 1:(5-10), and the second heating and leaching is performed at a temperature of 50-70° C. and for 30-60 min.

[0015] Preferably, the temperature of the rotary evaporator in step 3 is 50-70°C and the rotation speed is 40-60rpm.

[0016] Second, the present invention also provides a composition comprising the above-mentioned Ilex erythrorhizonii extract, comprising Ilex erythrorhizonii extract, coenzyme Q10, grape seed extract, soybean lecithin, and inulin.

[0017] Preferably, the components in the composition are as follows by mass:

[0018] 20-50 parts of iris extract, 5-15 parts of coenzyme Q10, 10-30 parts of grape seed extract, 5-20 parts of soybean lecithin, and 5-20 parts of inulin.

[0019] Thirdly, the present invention also provides the use of the above-mentioned Kuding extract in preparing blood lipid-lowering foods, health products or medicines.

[0020] Beneficial effects:

[0021] Compared with the existing technology, the present invention innovatively uses microwave treatment technology, so that the ethyl acetate, n-butanol and the components of Example 3 finally extracted can effectively reduce the blood lipid level of hyperlipidemia mice, improve serum indicators, restore mouse liver function, and reduce inflammation and oxidative stress levels. They can be used to prepare health products for improving hyperlipidemia, efficiently utilize Kudingcha components, reduce component loss, effectively improve yield, optimize the preparation process, and the method is reliable and simple, with broad market prospects.

[0022] The composition provided by the present invention can further improve the lipid-lowering effect of the composition through synergistic effects. Among them, coenzyme Q10: coenzyme Q10 is a fat-soluble antioxidant that can enhance heart function and improve the body's immunity, and enhance the overall effect of the composition through its antioxidant and auxiliary lipid-lowering effects. Grape seed extract: grape seed extract is rich in antioxidant ingredients such as proanthocyanidins, and has powerful antioxidant and anti-inflammatory effects. Soy lecithin: soy lecithin is a high-quality phospholipid source that can lower blood cholesterol and triglyceride levels, help prevent cardiovascular and cerebrovascular diseases, and provide additional lipid-lowering support. Inulin: dietary fiber can increase satiety and reduce calorie intake, and may enhance the overall effect of the composition by improving intestinal function and lowering blood lipid levels. However, the above components improve the overall lipid-lowering effect of the composition through synergistic effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a comparison chart of the effects of Kudingcha microphylla extract on the body shape of hyperlipidemic mice;

[0024] Figure 2 This is a comparison chart of the effect of Kudingcha microphylla extract on the body weight of hyperlipidemic mice;

[0025] Figure 3 This is a comparison chart of the effect of Kudingcha microphylla extract on fat weight (abdominal white fat WAT, epididymal fat EAT) in hyperlipidemic mice;

[0026] Figure 4 This is a comparison chart of the effects of the extract of Kudingcha microphylla on serum biochemical indices in hyperlipidemic mice;

[0027] Figure 5 This is a comparison chart of the effects of the extract of Kudingcha microphylla on serum immune factors in hyperlipidemic mice;

[0028] Figure 6 This is a comparison chart of the effect of the extract of Kudingcha microphylla on the liver of hyperlipidemic mice;

[0029] Figure 7 This is a comparison of the effects of the extract of Kudingcha microphylla on the liver of hyperlipidemic mice.

[0030] Figure 8 This is a comparison chart of the effect of Kudingcha microphylla extract on TC in the liver of hyperlipidemic mice;

[0031] Fig. 9 This is a comparison chart of the effect of Kudingcha microphylla extract on liver TG in hyperlipidemic mice;

[0032] Fig.10 This is a comparison chart of the effect of Kudingcha microphylla extract on ALT in the liver of hyperlipidemic mice;

[0033] Fig.11This is a comparison chart of the effect of Kudingcha microphylla extract on liver SOD in hyperlipidemic mice;

[0034] Fig.12 This is a comparison chart of the effects of Kudingcha microphylla extract on liver MDA in hyperlipidemia mice. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0036] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0037] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0038] Example 1 Preparation of a small leaf kuding extract:

[0039] (1) Raw material pretreatment: weigh high-quality, dry small-leaf Kuding tea leaves, crush and sieve to obtain tea powder; fully mix the obtained tea powder with water and then perform microwave treatment;

[0040] (2) Extraction: the product obtained in step 1 is further added with water and subjected to a first heating extraction, and filtered to obtain filtrate 1 and filter residue 1; the obtained filter residue 1 is added with ethanol aqueous solution and subjected to a second heating extraction, and filtered to obtain filtrate 2; the filtrate 1 and the filtrate 2 are mixed to obtain an extract;

[0041] (3) Concentration: Use a rotary evaporator to reduce the pressure and concentrate the extract until it becomes viscous;

[0042] (4) Organic solvent extraction: extracting and separating the concentrated extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and repeating each step three times to obtain a petroleum ether component, an ethyl acetate component, an n-butanol component, and a water-soluble component (the water-soluble component is from the extracted extract);

[0043] (5) Drying: The extracts of the various components obtained are dried under vacuum to obtain freeze-dried powders of petroleum ether component, ethyl acetate component, n-butanol component, and water-soluble component, which are mixed to obtain the extract of Ilex scoparia.

[0044] Sieve as described in step 1, through a 50-mesh sieve.

[0045] In step 1, the mass ratio of the ground tea leaves to water is 1:2, the microwave treatment power is 500 W, the temperature is controlled at 60° C., the total treatment time is 30 s, and intermittent treatment is adopted. After each treatment for 15 s, the next treatment is performed with an interval of 10 s.

[0046] In step 2, water is continued to be added, wherein the mass ratio of the product of step 1 to water is 1:10, and the first heating and leaching is performed at a temperature of 80° C. and a time of 60 min.

[0047] In step 2, the ethanol in the ethanol aqueous solution accounts for 70 vol%, the mass ratio of the filter residue 1 to the ethanol aqueous solution is 1:5, and the second heating and leaching is performed at a temperature of 50° C. and a time of 60 min.

[0048] The temperature of the rotary evaporator in step 3 is 50° C. and the rotation speed is 60 rpm.

[0049] Example 2 Preparation of a small leaf kuding extract:

[0050] (1) Raw material pretreatment: weigh high-quality, dry small-leaf Kuding tea leaves, crush and sieve to obtain tea powder; fully mix the obtained tea powder with water and then perform microwave treatment;

[0051] (2) Extraction: the product obtained in step 1 is further added with water and subjected to a first heating extraction, and filtered to obtain filtrate 1 and filter residue 1; the obtained filter residue 1 is added with ethanol aqueous solution and subjected to a second heating extraction, and filtered to obtain filtrate 2; the filtrate 1 and the filtrate 2 are mixed to obtain an extract;

[0052] (3) Concentration: Use a rotary evaporator to reduce the pressure and concentrate the extract until it becomes viscous;

[0053] (4) Organic solvent extraction: extracting and separating the concentrated extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and repeating each step three times to obtain a petroleum ether component, an ethyl acetate component, an n-butanol component, and a water-soluble component (the water-soluble component is from the extracted extract);

[0054] (5) Drying: The extracts of the various components obtained are dried under vacuum to obtain freeze-dried powders of petroleum ether component, ethyl acetate component, n-butanol component, and water-soluble component, which are mixed to obtain the extract of Ilex scoparia.

[0055] Sieve as described in step 1, and pass through a 100 mesh sieve.

[0056] In step 1, the mass ratio of the ground tea leaves to water is 1:3, the microwave treatment power is 700 W, the temperature is controlled at 80° C., the total treatment time is 30 s, and intermittent treatment is adopted. After each treatment for 15 s, the next treatment is carried out with an interval of 10 s.

[0057] In step 2, water is continued to be added, wherein the mass ratio of the product of step 1 to water is 1:20, and the first heating and leaching is performed at a temperature of 90° C. and a time of 30 min.

[0058] In step 2, the ethanol in the ethanol aqueous solution accounts for 80 vol%, the mass ratio of the filter residue 1 to the ethanol aqueous solution is 1:10, and the second heating and leaching is performed at a temperature of 70° C. and a time of 30 min.

[0059] The temperature of the rotary evaporator in step 3 is 70° C. and the rotation speed is 40 rpm.

[0060] Example 3 A composition containing a small leaf kuding extract

[0061] Including bitter melon extract, coenzyme Q10, grape seed extract, soy lecithin, and inulin.

[0062] The components in the composition are calculated by weight:

[0063] 20 parts of the Ilex erythrorhizome extract prepared in Example 2, 15 parts of coenzyme Q10, 30 parts of grape seed extract, 20 parts of soybean lecithin, and 20 parts of inulin.

[0064] Example 4 A composition containing a small leaf kuding extract

[0065] Including bitter melon extract, coenzyme Q10, grape seed extract, soy lecithin, and inulin.

[0066] The components in the composition are calculated by weight:

[0067] 50 parts of the Ilex erythrorhizome extract prepared in Example 2, 5 parts of coenzyme Q10, 10 parts of grape seed extract, 5 parts of soybean lecithin, and 5 parts of inulin.

[0068] Comparative Example 1 A composition:

[0069] Includes coenzyme Q10, grape seed extract, soy lecithin, and inulin.

[0070] The components in the composition are calculated by weight:

[0071] Coenzyme Q10 5 parts, grape seed extract 10 parts, soy lecithin 5 parts, inulin 5 parts.

[0072] Comparative Example 2: A composition:

[0073] Including bitter melon extract, grape seed extract, soy lecithin, and inulin.

[0074] The components in the composition are calculated by weight:

[0075] 50 parts of the Ilex erythrorhizome extract prepared in Example 2, 10 parts of grape seed extract, 5 parts of soybean lecithin, and 5 parts of inulin.

[0076] Comparative Example 3: A composition:

[0077] Including bitter melon extract, coenzyme Q10, soy lecithin, and inulin.

[0078] The components in the composition are calculated by weight:

[0079] 50 parts of the Ilex erythrorhizome extract prepared in Example 2, 5 parts of coenzyme Q10, 5 parts of soybean lecithin, and 5 parts of inulin.

[0080] Comparative Example 4: a composition:

[0081] Including bitter melon extract, coenzyme Q10, grape seed extract, and inulin.

[0082] The components in the composition are calculated by weight:

[0083] 50 parts of the Ilex erythrorhizome extract prepared in Example 2, 5 parts of coenzyme Q10, 10 parts of grape seed extract, and 5 parts of inulin.

[0084] Comparative Example 5: a composition:

[0085] Including bitter melon extract, coenzyme Q10, grape seed extract, and soy lecithin.

[0086] The components in the composition are calculated by weight:

[0087] 50 parts of the Ilex erythrorhizome extract prepared in Example 2, 5 parts of coenzyme Q10, 10 parts of grape seed extract, and 5 parts of soybean lecithin.

[0088] The effects of the extracts or compositions prepared in the above embodiments and comparative examples on lowering blood lipids were tested using a hyperlipidemia mouse experiment, as follows:

[0089] 1. Experimental Methods

[0090] 1. Animal modeling and administration of Kudingcha chinensis to improve hyperlipidemia

[0091] 110 5-week-old male C57BL / 6J mice (18-22g) were adaptively fed for one week; 110 C57BL / 6J mice were divided into a normal group, a model control group, all the above-mentioned Example 1-4 groups and Comparative Example 1-5 groups according to a computer random number table, with 10 mice in each group. Except for the normal group fed with ordinary feed, all animals were fed with high-fat feed, and all animals had free access to water. Each group was gavaged while feeding. The normal group and the model group were gavaged with physiological saline (0.1mL / 10g / d), and the other groups were gavaged with the corresponding components, all at a dose of 500mg / kg / d, until the end of the 12-week experiment.

[0092] 2. Collection and processing of serum

[0093] At the end of the experiment, blood was collected from the orbital venous plexus of the mice, centrifuged at 3000rpm for 10min, and the supernatant was separated. The total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), glucose (GLU), tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and lipopolysaccharide (LPS) in the serum of each mouse were measured, and the differences among the groups were statistically analyzed.

[0094] 3. Collection and processing of tissues

[0095] The liver, abdominal white fat and epididymal fat tissue of mice were removed and weighed and recorded, and fixed in tissue fixative. Part of the liver tissue was taken to make HE and Oil Red O sections to observe the distribution of lipid droplets in the liver tissue.

[0096] 4. Liver biochemical index detection

[0097] Weigh an appropriate amount of liver tissue, add 9 times the volume of normal saline according to the ratio of weight (g): volume (mL) = 1:9, and prepare a 10% homogenate by mechanical homogenization under ice water bath conditions, centrifuge at 4°C, 2500r / min for 10min to obtain the supernatant. CHOD-PAP method, GPO-PAP method and alanine substrate method were used to detect the TC, TG and ALT contents in the liver of each group of mice.

[0098] 5. Detection of oxidative stress factor levels

[0099] The WST-1 method and TBA method were used to determine the contents of SOD and MDA in the liver according to the kit operation.

[0100] 2. Experimental Results

[0101] In the above experimental results, the experimental results of Example 3 and Example 4 are similar, the experimental results of Comparative Examples 2, 3, and 5 are similar, the experimental results of Comparative Example 4, Example 1, and Example 2 are similar to the positive drug group, and the experimental results of Comparative Example 1 are similar to the model group.

[0102] 1. Effect of Kudingcha microphylla extract on body weight and fat in mice

[0103] After eight weeks of intragastric administration, the mice in the model group were in a depressed state, had decreased mobility, and had a significant weight gain effect compared with the blank group. Figure 1 , where A is the normal group; B is the model group; C is the positive drug group; D is the Example 1 group; E is the Comparative Example 2 group; and F is the Example 3 group. The Example 1 group, the Comparative Example 2 group, and the Example 3 group had a decrease in body weight, which was significantly different from the model group. Consistent with the trend of weight loss, the abdominal white fat (WAT) and epididymal fat weight (EAT) of the Example 1 group, the Comparative Example 2 group, and the Example 3 group were significantly lower than those of the model group. See for details Figure 2-3 , Note: Compared with the normal group, significant*p<0.05, extremely significant**p<0.01; compared with the model group, significant # p<0.05, extremely significant ## p<0.01.

[0104] 2. Effects of Kudingcha microphylla extract on serum biochemical parameters

[0105] After 8 weeks, the levels of TC, TG and LDL-C in the serum of the mice in the model group were significantly higher than those in the control group, indicating that the mice had been modeled into hyperlipidemia mice. At the same time, the levels of Glu, TNF-α, IL-6, IL-1β and LPS in the model group were significantly increased. Compared with the model group, the levels of serum TC, TG, Glu, TNF-α, IL-6, IL-1β and LPS in the Example 1 group, the Comparative Example 2 group and the Example 3 group were significantly reduced after oral administration. Figure 4-5 . This indicates that the extract of Kudingcha microphylla has a significant effect on lowering blood lipids. Note: Compared with the normal group, significant*p<0.05, extremely significant**p<0.01; compared with the model group, significant # p<0.05, extremely significant ## p<0.01.

[0106] 3. Effect of Kudingcha microphylla extract on liver lipids

[0107] The results of H&E staining showed that the liver tissue structure of the control group was normal without obvious lipid droplets, while the model group had a large number of vacuolated lipid droplets. After oral gavage intervention, the lipid droplets of Example 1, Comparative Example 2, and Example 3 groups were significantly reduced, effectively improving liver damage. The results of Oil Red O staining showed that the model group had significantly more lipid droplet deposition than the control group, and Example 1, Comparative Example 2, and Example 3 groups significantly reduced the lipid droplet deposition phenomenon in liver tissue, as shown in detail. Figure 6-7This indicates that the Example 1, Comparative Example 2 and Example 3 of the small leaf Kudingcha tea have protective effects on the liver cells of hyperlipidemia mice. Note: A normal group; B model group; C positive drug group; D Example 1 group; E Comparative Example 2 group; F Example 3 group.

[0108] 4. Effects of Kudingcha microphylla extract on liver biochemical indexes and oxidative stress factor levels

[0109] From the results, it can be seen that the small-leaf Kudingcha Example 1 group, the comparative example 2 group and the Example 3 group have an improving effect on liver biochemical indexes and oxidative stress factor levels.

[0110] The above results show that the composition provided by the present invention achieves the effect of improving the lipid-lowering effect through synergistic action: the interaction of multiple active ingredients: the small leaf bitter extract, coenzyme Q10, grape seed extract, soy lecithin and dietary fiber each contain multiple active ingredients, which may produce synergistic effects in the human body and jointly promote the reduction of blood lipids. The tea polyphenols and flavonoids in the small leaf bitter extract combined with the antioxidant effect of coenzyme Q10 may more effectively remove free radicals in the body and reduce the generation of lipid peroxidation products, thereby reducing blood lipid levels. Complementarity of metabolic pathways: Different components may affect blood lipid levels through different metabolic pathways. The small leaf bitter extract may mainly reduce blood lipids by inhibiting the synthesis and absorption of cholesterol, while soy lecithin may play a role by promoting the transport and metabolism of cholesterol. The complementarity of these metabolic pathways may make the composition show a stronger effect in reducing blood lipids. The role of dietary fiber: As an important component of the composition, dietary fiber can increase stool volume, soften stool, and promote intestinal peristalsis, thereby improving intestinal health. Good intestinal health helps to reduce the reabsorption of cholesterol in the intestine, thereby reducing blood lipid levels. Prebiotic effect: Some dietary fibers (such as inulin) have prebiotic effects and can promote the growth and reproduction of beneficial bacteria in the intestine. These beneficial bacteria can produce substances such as short-chain fatty acids, which further reduce blood lipid levels. Enhanced antioxidant capacity: Microphylla kuding extract, coenzyme Q10 and grape seed extract all have strong antioxidant effects. They can scavenge free radicals in the body and reduce the production of lipid peroxidation products, thereby reducing blood lipid levels. When these ingredients are combined together, their antioxidant effects may be further enhanced, thereby more effectively reducing blood lipids. Multiple pathways to regulate lipid metabolism: Different components in the composition may regulate lipid metabolism through multiple pathways. Microphylla kuding extract may reduce blood lipids by inhibiting the synthesis and absorption of cholesterol; soy lecithin may play a role by promoting the transport and metabolism of cholesterol; and coenzyme Q10 may reduce blood lipids by improving the energy metabolism efficiency of mitochondria. The regulation of these multiple pathways may make the composition show a stronger effect in lowering blood lipids. Improved pharmacokinetic properties: Certain ingredients in the composition (such as soybean lecithin) may have the effect of improving pharmacokinetic properties, such as increasing the solubility and stability of the drug, prolonging the retention time of the drug in the body, etc. These effects may make the effective ingredients such as the small leaf kuding extract more easily absorbed and utilized in the body, thereby enhancing its lipid-lowering effect.

[0111] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0112] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A small leaf kuding extract, characterized in that: The preparation method comprises the following steps: (1) Raw material pretreatment: weigh high-quality, dry small-leaf Kuding tea leaves, crush and sieve to obtain tea powder; fully mix the obtained tea powder with water and then perform microwave treatment; (2) Extraction: the product obtained in step 1 is further added with water and subjected to a first heating extraction, and filtered to obtain filtrate 1 and filter residue 1; the obtained filter residue 1 is added with ethanol aqueous solution and subjected to a second heating extraction, and filtered to obtain filtrate 2; the filtrate 1 and the filtrate 2 are mixed to obtain an extract; (3) Concentration: Use a rotary evaporator to reduce the pressure and concentrate the extract until it becomes viscous; (4) Organic solvent extraction: extracting and separating the concentrated extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and repeating each step three times to obtain a petroleum ether component, an ethyl acetate component, an n-butanol component, and a water-soluble component in sequence; (5) Drying: The extracts of the components are dried under vacuum to obtain freeze-dried powders of petroleum ether component, ethyl acetate component, n-butanol component and water-soluble component, which are mixed to obtain the extract of Ilex scoparia.

2. The Ipomoea australis extract according to claim 1, characterized in that: Sieve as described in step 1, through a 50-100 mesh sieve.

3. The Ipomoea australis extract according to claim 1, characterized in that: In step 1, the mass ratio of the ground tea to water is 1:(2-3), the microwave treatment power is 500-700W, the temperature is controlled at 60-80°C, the total treatment time is 30-60s, and intermittent treatment is adopted, with an interval of 10s after each treatment of 15s before the next treatment.

4. The Ipomoea australis extract according to claim 1, characterized in that: In step 2, water is continued to be added, wherein the mass ratio of the product of step 1 to water is 1:(10-20), and the first heating and leaching is performed at a temperature of 80-90° C. and a time of 30-60 min.

5. The Ipomoea australis extract according to claim 1, characterized in that: In step 2, the ethanol in the ethanol aqueous solution accounts for 70-80 vol%, the mass ratio of the filter residue 1 to the ethanol aqueous solution is 1:(5-10), and the second heating and leaching is performed at a temperature of 50-70° C. and a time of 30-60 min.

6. The Ipomoea australis extract according to claim 1, characterized in that: The temperature of the rotary evaporator in step 3 is 50-70°C and the rotation speed is 40-60rpm.

7. A composition comprising the extract of Ilex scoparia according to any one of claims 1 to 6, characterized in that: Including bitter melon extract, coenzyme Q10, grape seed extract, soy lecithin, and inulin.

8. The composition according to claim 7, characterized in that: The components in the composition are calculated by weight: 20-50 parts of iris extract, 5-15 parts of coenzyme Q10, 10-30 parts of grape seed extract, 5-20 parts of soybean lecithin, and 5-20 parts of inulin.

9. Use of the Ilex erythrorhizoma Ilex extract according to any one of claims 1 to 6 in the preparation of lipid-lowering foods, health products or medicines.

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