Fermentation method and application of rhizoma polygonati
Through the fermentation method of Polygonatum, the problem of inconvenience in application of traditional Polygonatum is solved. Through microbial fermentation treatment, the Polygonatum fermentation broth formed has gentle medicinal effects and is suitable for a variety of health and treatment needs.
Patent Information
- Application Number
- CN202510153926.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2025-05-06
AI Technical Summary
The traditional way of using Polygonatum cannot change its active ingredients and is not convenient for application in different fields.
The fermentation method of Polygonatum is adopted, including drying, crushing, and fermenting fresh Polygonatum, and fermenting it through microbial fermentation treatment to form a Polygonatum fermentation broth.
Through fermentation treatment, Polygonatum has mild effect, which is more conducive to the human body's absorption, and has weakened toxicity or irritation. It is suitable for nourishing and treating symptoms of dry lung cough and kidney deficiency.
Smart Images
Figure CN119925519A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of polygonatum fermented liquid, and specifically to a polygonatum fermentation method and application. Background Art
[0002] Polygonatum sibiricum is a perennial herb of the Asparagaceae family and the genus Polygonatum. It has a root-like stem with swollen nodes, one thick end and the other thin end of the internode, and the thick end has short branches. Polygonatum sibiricum grows in the edge of mountain forests, in thickets or in semi-shaded areas on hillsides, and is distributed in various areas north of the Yangtze River. The fleshy rhizomes can be eaten raw, and are crisp, tender, sweet, and refreshing to eat. They can also be fried, cooked in soup, steamed, made into porridge, soaked in wine, and used as medicinal food. As medicine, it can be used for symptoms such as weak spleen and stomach, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, lack of essence and blood, internal heat and thirst, and for the treatment of tuberculosis, ringworm, etc.
[0003] Traditionally, Polygonatum sibiricum is usually ground into fine powder or directly sliced, and then taken by soaking in water or boiling. However, the effective ingredients of Polygonatum sibiricum itself cannot be changed during the soaking and boiling process, and Polygonatum sibiricum cannot be modified, which is inconvenient for the application of Polygonatum sibiricum in different fields. Therefore, a fermentation method and application of Polygonatum sibiricum are proposed to address the above problems. Summary of the invention
[0004] The purpose of the present application is to provide a fermentation method and application of polygonatum sibiricum to solve the problem of inconvenience in applying polygonatum sibiricum in different fields.
[0005] To achieve the above objectives, this application provides the following technical solutions: A fermentation method and application of polygonatum sibiricum, comprising the steps of: drying and dehydrating fresh polygonatum sibiricum to form dry polygonatum sibiricum, then putting the dry polygonatum sibiricum into a pulverizing device for pulverizing, sieving polygonatum sibiricum powder after pulverizing, separating polygonatum fine powder from polygonatum sibiricum particles, collecting the polygonatum fine powder, and feeding the polygonatum sibiricum particles and polygonatum blocks into the pulverizing device for pulverizing; Step 2: Take the Huangjing fine powder according to the prescription and put it into the fermentation tank, then add 15 times the amount of water as the Huangjing powder in the prescription into the fermentation tank, and then stir evenly; Step 3: Take 3% of the amount of amylase and saccharifying enzyme in the yellow fine powder prescription, and then dissolve them in water respectively, and then add the amylase and saccharifying enzyme mixed with water into the fermentation tank in turn, and start the fermentation tank to stir and enzymolyze the liquid; Step 4: Take 1% of the amount of neutral protease in the yellow fine powder prescription, and then dissolve it with water. Then add the neutral protease mixed with water into the fermentation tank, open the steam valve and continue to stir the enzymolysis; Step 5: After the enzymatic hydrolysis is completed, the steam valve of the fermentation tank is opened to heat the fermentation tank to inactivate the liquid. After the enzymatic hydrolysis is completed, the steam valve is closed, and the circulating water valve is opened to cool the fermentation tank to form an enzymatic hydrolyzate; Step 6: adding an appropriate amount of one or more of Lactobacillus plantarum, Bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus reuteri to the enzymolysis solution in the fermentation tank, inoculating the enzymolysis solution with bacterial strains, opening the steam valve for culturing, then sampling the enzymolysis solution in the fermentation tank, detecting the pH value of the enzymolysis solution sample, and stopping the fermentation when the pH value is less than 4; Step 7: sending the fermented mixed liquid to a tubular centrifuge, separating the mixed liquid into solid and liquid by the tubular centrifuge, collecting the centrifuge, and finally taking out the solid and drying it for collection; Step 8: heating the centrifuge to boil, and then cooling to room temperature, and obtaining a finished product of polygonatum fermented liquid after the centrifuge is cooled to room temperature; Step nine: filtering the polygonatum fermented liquid, passing the filtered polygonatum fermented liquid into a filling machine, and then filling it according to the standard of 30 ml, and then sealing the container of the filled polygonatum fermented liquid, and finally directly loading the unfilled polygonatum fermented liquid into the raw material barrel for refrigerated storage; Step 10: Send the filled Polygonatum sibiricum fermented liquid into the sterilization equipment for sterilization and leak detection, package the Polygonatum sibiricum fermented liquid after it is cooled to room temperature, and finally store the packaged Polygonatum sibiricum fermented liquid in the warehouse.
[0006] Preferably, during the processing of polygonatum, fresh polygonatum is cleaned with clean water, and then the water on the surface of polygonatum is wiped dry, and then the fresh polygonatum is placed in a ventilated and dry room to air-dry, and then the air-dried polygonatum is placed in an electric drying oven, and the polygonatum is dried at 45-50°C in the electric drying oven for more than 12 hours to make dry polygonatum, and then the dry polygonatum is cut into pieces and crushed by a grinder to make polygonatum fine powder.
[0007] Preferably, the temperature in the fermentation tank in step 3 is controlled at 50° C., and the stirring and enzymolysis time is 3 hours.
[0008] Preferably, the temperature in the fermentation tank in step 4 is controlled at 55° C., and the stirring and enzymolysis time is 2 hours.
[0009] Preferably, during the enzymatic inactivation in step 5, the temperature in the fermentation tank rises to 115° C. and is maintained for 20 minutes, the temperature of the enzymatic hydrolyzate is 20-24° C., and the pressure in the tank is 0.
[0010] Preferably, in step six, the temperature in the fermentation tank is controlled at 37.5 degrees Celsius and the culture time is 60-72 hours.
[0011] Preferably, the centrifuge collected in step seven is 12-13 times the yellow fine powder during processing.
[0012] Preferably, the heating and boiling time of the centrifuge in step eight is 10 minutes. After cooling to room temperature, the centrifuge should be 12 times the amount of yellow fine powder used in processing. If the centrifuge is less than 12 times the amount of yellow fine powder used, add an appropriate amount of boiled purified water to the centrifuge to make the centrifuge reach 12 times the amount of yellow fine powder used in processing.
[0013] Preferably, the sterilization equipment heats the Polygonatum sibiricum fermented liquid at a temperature of 100° C. for 30 minutes, thereby sterilizing and leak-checking the Polygonatum sibiricum fermented liquid.
[0014] Preferably, the fermented polygonatum sibiricum liquid is used in health products and traditional Chinese medicines.
[0015] Compared with the prior art, the beneficial effects of this application are: In the present application, the fermented Polygonatum sibiricum liquid nourishes the body's qi and blood to achieve the purpose of nourishing the internal organs, enhances the spleen and stomach's transportation and transformation functions, and moisturizes the lungs at the same time. It is suitable for treating symptoms such as dry lung cough, and can be used to treat symptoms caused by kidney deficiency. The fermented Polygonatum sibiricum fermented liquid can be produced by microbial fermentation. Fermented Chinese medicine itself has a high technical level and threshold. The fermented Chinese medicine technology can achieve controllable production process, can ensure the product quality of the obtained product, can be prepared into liquid fermentation preparations, and is convenient for various Chinese medicines to be used. Organisms can decompose some active ingredients in Chinese medicines, thereby resulting in mild efficacy of Chinese medicines, which is more conducive to human absorption. The microorganisms used for fermenting Chinese medicines are strains that are beneficial to the animal body. They themselves have the functions of enhancing immunity, regulating intestinal balance, and improving production performance. After the fermentation treatment, the toxicity or irritation of the medicinal materials is significantly reduced. This is because under the action of microorganisms, the structure of the toxic components changes and becomes a low-toxic or even non-toxic substance, reducing the irritation of the Chinese medicine. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 This is a schematic diagram of the fermentation formula of polygonatum sibiricum of the present application; Figure 2 This is a schematic diagram of the process of the Polygonatum sibiricum fermentation broth of this application. DETAILED DESCRIPTION
[0017] See also Figure 1-2 , this application provides a technical solution: A fermentation method and application of polygonatum sibiricum, comprising the following steps: Step 1: During the processing of polygonatum, fresh polygonatum is cleaned with clean water, and then the water on the surface of polygonatum is wiped dry, and then the fresh polygonatum is air-dried in a ventilated and dry room, and then the air-dried polygonatum is placed in an electric drying oven, and the polygonatum is dried at 45-50°C for more than 12 hours by the electric drying oven to make dry polygonatum, and then the dry polygonatum is cut into pieces, crushed by a grinder, the polygonatum powder is sieved, the polygonatum fine powder is separated from the polygonatum particles, and then the polygonatum fine powder is collected to make the polygonatum fine powder, and the polygonatum particles and the polygonatum blocks are sent to the crushing device for crushing; Step 2: Take the Huangjing fine powder according to the prescription and put it into the fermentation tank, then add 15 times the amount of water as the Huangjing powder in the prescription into the fermentation tank, and then stir evenly; Step 3: Take 3% of the amount of amylase and saccharifying enzyme in the yellow fine powder prescription, and then dissolve them in water respectively. Then, add the amylase and saccharifying enzyme mixed with water into the fermentation tank in turn, start the fermentation tank to stir the liquid, and then open the steam valve, control the temperature in the fermentation tank at 50°C, and stir the enzymolysis for 3 hours; Step 4: Take 1% of the amount of neutral protease in the yellow fine powder prescription, and then dissolve it with water. Then add the neutral protease mixed with water into the fermentation tank, and then control the temperature in the fermentation tank at 55°C, and continue to stir and enzymolyze for 2 hours; Step 5: Open the steam valve of the fermenter to raise the temperature in the fermenter to 115°C and maintain it for 20 minutes to inactivate the liquid. After the enzymatic inactivation is completed, close the steam valve. When the pressure in the fermenter is 0, open the circulating water valve to reduce the temperature of the fermenter to 20-24°C to form an enzymatic solution. Step 6: adding an appropriate amount of one or more of Lactobacillus plantarum, Bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus reuteri to the enzymolysis solution in the fermentation tank, inoculating the enzymolysis solution with bacteria, opening the steam valve, and controlling the temperature in the fermentation tank at 37.5° C., culturing for 60-72 hours, then sampling the enzymolysis solution in the fermentation tank, detecting the pH value of the enzymolysis solution sample, and stopping the fermentation when the pH value is less than 4; Step 7: Send the fermented mixed liquid to a tubular centrifuge, separate the mixed liquid into solid and liquid by the tubular centrifuge, collect the centrifuge, the collected centrifuge is 12-13 times the yellow fine powder during processing, and finally take out the solid and dry it for collection; Step 8: Heat and boil the centrifuge for 10 minutes, then cool to room temperature. After the centrifuge is cooled to room temperature, the centrifuge should be 12 times the amount of yellow fine powder used in processing. If the centrifuge is less than 12 times the amount of yellow fine powder used, add an appropriate amount of boiled purified water to the centrifuge to make the centrifuge reach 12 times the amount of yellow fine powder used in processing, thereby obtaining a finished product of polygonatum fermented liquid; Step nine: filtering the polygonatum fermented liquid, passing the filtered polygonatum fermented liquid into a filling machine, and then filling it according to the standard of 30 ml, and then sealing the container of the filled polygonatum fermented liquid, and finally directly putting the unfilled polygonatum fermented liquid into the raw material barrel for refrigerated storage; Step 10: Send the filled Polygonatum sibiricum fermented liquid into the sterilization equipment, heat the filled Polygonatum sibiricum fermented liquid to 100°C and maintain it for 30 minutes, so as to sterilize and leak-check the Polygonatum sibiricum fermented liquid, package it after it cools to room temperature, and finally store the packaged Polygonatum sibiricum fermented liquid in the warehouse.
[0018] Polygonatum fermented liquid is used in health care products and traditional Chinese medicine. Polygonatum fermented liquid nourishes the body's qi and blood to achieve the purpose of nourishing internal organs, enhances the spleen and stomach's transportation and transformation functions, and moisturizes the lungs. It is suitable for treating dry lung cough and other symptoms, and can be used to treat symptoms caused by kidney deficiency. The fermented Polygonatum fermented liquid can be produced by microbial fermentation. Fermented Chinese medicine itself has a high technical level and threshold. The fermentation Chinese medicine technology can achieve controllable production process and ensure the product quality of the obtained product. It can be prepared into liquid fermentation preparations for the use of various Chinese medicines. Organisms can decompose some active ingredients in Chinese medicines, resulting in mild efficacy of Chinese medicines, which is more conducive to human absorption. The microorganisms used for fermenting Chinese medicines are strains that are beneficial to the animal body. They themselves have the functions of enhancing immunity, regulating intestinal balance, and improving production performance. After the fermentation treatment, the toxicity or irritation of the medicinal materials is significantly reduced. This is because under the action of microorganisms, the structure of the toxic components changes and becomes low-toxic or even non-toxic substances, reducing the irritation of Chinese medicines.
[0019] This article uses specific examples to illustrate the principles and implementation methods of this application. The above examples are only used to help understand the method and its core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression and the objective existence of infinite specific structures, ordinary technicians in this technical field can make several improvements, modifications or changes without departing from the principles of this application, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the scope of protection of this application.
Claims
1. A fermentation method and application of Polygonatum sibiricum, characterized in that: The following steps are involved: Step 1: drying and dehydrating fresh polygonatum to form dry polygonatum, and then putting the dry polygonatum into a grinding device for grinding. After the grinding is completed, the polygonatum powder is sieved to separate the polygonatum fine powder from the polygonatum particles, and then the polygonatum fine powder is collected, and the polygonatum particles and polygonatum blocks are sent to the grinding device for grinding; Step 2: Take the Huangjing fine powder according to the prescription and put it into the fermentation tank, then add 15 times the amount of water as the Huangjing powder in the prescription into the fermentation tank, and then stir evenly; Step 3: Take 3% of the amount of amylase and saccharifying enzyme in the yellow fine powder prescription, and then dissolve them in water respectively, and then add the amylase and saccharifying enzyme mixed with water into the fermentation tank in turn, and start the fermentation tank to stir and enzymolyze the liquid; Step 4: Take 1% of the amount of neutral protease in the yellow fine powder prescription, and then dissolve it with water. Then add the neutral protease mixed with water into the fermentation tank, open the steam valve and continue to stir the enzymolysis; Step 5: After the enzymatic hydrolysis is completed, the steam valve of the fermentation tank is opened to heat the fermentation tank to inactivate the liquid. After the enzymatic hydrolysis is completed, the steam valve is closed, and the circulating water valve is opened to cool the fermentation tank to form an enzymatic hydrolyzate; Step 6: adding an appropriate amount of one or more of Lactobacillus plantarum, Bifidobacterium lactis, Lactobacillus rhamnosus and Lactobacillus reuteri to the enzymolysis solution in the fermentation tank, inoculating the enzymolysis solution with bacterial strains, opening the steam valve for culturing, then sampling the enzymolysis solution in the fermentation tank, detecting the pH value of the enzymolysis solution sample, and stopping the fermentation when the pH value is less than 4; Step 7: sending the fermented mixed liquid to a tubular centrifuge, separating the mixed liquid into solid and liquid by the tubular centrifuge, collecting the centrifuge, and finally taking out the solid and drying it for collection; Step 8: heating the centrifuge to boil, and then cooling to room temperature, and obtaining a finished product of polygonatum fermented liquid after the centrifuge is cooled to room temperature; Step nine: filtering the polygonatum fermented liquid, passing the filtered polygonatum fermented liquid into a filling machine, and then filling it according to the standard of 30 ml, and then sealing the container of the filled polygonatum fermented liquid, and finally directly putting the unfilled polygonatum fermented liquid into the raw material barrel for refrigerated storage; Step 10: Send the filled Polygonatum sibiricum fermented liquid into the sterilization equipment for sterilization and leak detection, package the Polygonatum sibiricum fermented liquid after it is cooled to room temperature, and finally store the packaged Polygonatum sibiricum fermented liquid in the warehouse.
2. The fermentation method of polygonatum according to claim 1, characterized in that: During the processing of Polygonatum sibiricum, the fresh Polygonatum sibiricum is cleaned with clean water, and then the water on the surface of the Polygonatum sibiricum is wiped dry. Then the fresh Polygonatum sibiricum is placed in a ventilated and dry room to air-dry. Then the air-dried Polygonatum sibiricum is placed in an electric drying oven and dried at 45-50°C for more than 12 hours in the electric drying oven to make dry Polygonatum sibiricum. Then the dry Polygonatum sibiricum is cut into pieces and crushed by a grinder to make fine Polygonatum sibiricum powder.
3. The fermentation method of polygonatum according to claim 1, characterized in that: The temperature in the fermentation tank in step 3 is controlled at 50° C., and the stirring and enzymolysis time is 3 hours.
4. The fermentation method of polygonatum according to claim 1, characterized in that: The temperature in the fermentation tank in step 4 is controlled at 55° C., and the stirring and enzymolysis time is 2 hours.
5. The fermentation method of polygonatum according to claim 1, characterized in that: During the enzymatic inactivation in step 5, the temperature in the fermentation tank rises to 115° C. and is maintained for 20 minutes. The temperature of the enzymatic hydrolyzate is 20-24° C., and the pressure in the tank is 0.
6. The fermentation method of polygonatum according to claim 1, characterized in that: In step six, the temperature in the fermentation tank is controlled at 37.5 degrees Celsius and the culture time is 60-72 hours.
7. The fermentation method of polygonatum according to claim 1, characterized in that: The centrifuge collected in step 7 is 12-13 times the yellow fine powder during processing.
8. The fermentation method of polygonatum according to claim 1, characterized in that: In step eight, the heating and boiling time of the centrifuge liquid is 10 minutes. After cooling to room temperature, the centrifuge liquid should be 12 times the amount of yellow fine powder used in processing. If the centrifuge liquid is less than 12 times the amount of yellow fine powder used, add an appropriate amount of boiled purified water to the centrifuge liquid to make the centrifuge liquid reach 12 times the amount of yellow fine powder used in processing.
9. The fermentation method of polygonatum according to claim 1, characterized in that: The sterilization equipment heats the Polygonatum sibiricum fermented liquid at a temperature of 100°C for 30 minutes, thereby sterilizing and leak-checking the Polygonatum sibiricum fermented liquid.
10. The use of a fermentation method of polygonatum according to any one of claims 1 to 9, characterized in that: Polygonatum sibiricum fermented liquid is used in health products and traditional Chinese medicines.