Heat stress resistant traditional Chinese medicine composition and application thereof

Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an anti-heat stress traditional Chinese medicine composition and application thereof. The traditional Chinese medicine composition for resisting heat stress comprises the following components in parts by mass: 6-12 parts of agastache rugosus, 4-8 parts of rhizoma smilacis glabrae, 4-8 parts of rhizoma phragmitis, 4-8 parts of caulis lonicerae, 1-5 parts of herba schizonepetae, 1-5 parts of herba patriniae, 1-5 parts of lalang grass rhizome, 1-5 parts of lophatherum gracile, 1-5 parts of felwort, 1-5 parts of pericarpium citri reticulatae, 1-5 parts of gypsum, 1-5 parts of rhizoma anemarrhenae, 1-5 parts of scandent hop and 1-5 parts of rhizoma acori graminei. The heat-stress-resistant traditional Chinese medicine composition disclosed by the invention has the effects of relieving exterior syndrome with pungent and cool drugs, clearing heat and drying dampness, ventilating lung qi, protecting heart and liver, inducing resuscitation and inducing resuscitation, and can effectively relieve heat stress reaction and improve physiological and biochemical indexes of beef cattle under the condition of heat stress.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and particularly relates to an anti-heat stress traditional Chinese medicine composition and application thereof. Background Art

[0002] In recent years, heat stress has become a very harmful stressor in livestock and poultry farming. When animals are in a high temperature and high humidity environment, the heat load they generate exceeds their heat dissipation capacity, resulting in the body making a series of non-specific physiological responses. Pigs, poultry and ruminants are susceptible to heat stress due to their fast metabolic rate, increased basal metabolic heat production, fast growth and high production. For ruminants, the heat released during feed fermentation in the rumen will increase their metabolic heat production and endanger their ability to regulate body temperature. In beef cattle farming, physical prevention and control is the main means of combating heat stress. At this stage, temperature control facilities have some limitations. For example, the most commonly used spray cooling is simple and effective, but it consumes a lot of water, which will lead to increased environmental humidity and hinder normal evaporative heat dissipation. Therefore, it is crucial for the livestock industry to find and study effective drugs or additives to combat heat stress. Summary of the invention

[0003] In order to solve the above technical problems, the present invention provides an anti-heat stress Chinese medicine compound prescription, which is added to the daily feed of beef cattle in high temperature and high humidity seasons to alleviate the heat stress reaction of beef cattle.

[0004] The invention provides an anti-heat stress Chinese medicine composition, comprising the following components in parts by mass: 6-12 parts of Patchouli, 4-8 parts of Smilax glabra, 4-8 parts of Phragmites australis, 4-8 parts of Caulis Loniceraes, 1-5 parts of Schizonepeta tenuifolia, 1-5 parts of Patrinia scabra, 1-5 parts of Imperata cylindrica, 1-5 parts of Lophatherum gracile, 1-5 parts of Gentiana scabra, 1-5 parts of Citrus reticulata, 1-5 parts of Gypsum, 1-5 parts of Anemarrhena asphodeloides, 1-5 parts of Humulus japonicus and 1-5 parts of Acorus calamus.

[0005] Preferably, 9 of Patchouli, 6 of Smilax glabra, 6 of Phragmites australis, 6 of Caulis Loniceraes, 3 of Schizonepeta tenuifolia, 3 of Patrinia scabra, 3 of Imperata cylindrica, 3 of Lophatherum gracile, 3 of Gentiana scabra, 3 of Citrus reticulata, 3 of Gypsum, 3 of Anemarrhena asphodeloides, 3 of Humulus japonicus, and 3 of Acorus calamus.

[0006] Furthermore, the anti-heat stress Chinese medicine composition is a powder.

[0007] The present invention also provides application of the heat stress resistant traditional Chinese medicine composition in preparing ruminant feed.

[0008] Furthermore, the ruminant is a beef cattle.

[0009] The present invention also provides a method for raising beef cattle, comprising: adding 200-300g of the anti-heat stress Chinese medicine composition to the beef cattle feed every day when the temperature and humidity index (THI) of the cattle house is above 72. The temperature and humidity index is calculated by the following formula: temperature and humidity index (THI) = td-(0.55-0.55RH)×(td-58); td=T×9 / 5+32, where: td is Fahrenheit temperature (℉), T is Celsius temperature (℃), and RH is relative humidity (%).

[0010] Furthermore, when the temperature and humidity index (THI) of the cowshed is above 79, 250 g of the anti-heat stress Chinese medicine composition is added to the beef cattle feed every day.

[0011] Compared with the prior art, the beneficial effects of the present invention include at least:

[0012] 1. The anti-heat stress Chinese medicine composition of the present invention has the effects of relieving exterior symptoms with pungent and cool properties, clearing away heat and drying dampness, promoting the flow of lung qi, protecting the heart and liver, refreshing the mind and invigorating the mind, and can effectively alleviate heat stress reactions.

[0013] 2. The anti-heat stress Chinese medicine composition of the present invention can improve the physiological and biochemical indicators of beef cattle under heat stress conditions, such as increasing the daily weight gain of beef cattle, reducing the respiratory rate of beef cattle, and alleviating a series of adverse effects such as liver damage and oxidative damage caused by heat stress on beef cattle. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is the temperature and humidity index of the cattle farm during the experiment. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical scheme and beneficial effects of the present invention clearer, the present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. Examples of the embodiments are shown in the accompanying drawings. It should be understood that the specific embodiments described in the following embodiments of the present invention are only exemplary descriptions of the specific embodiments of the present invention, and are intended to be used to explain the present invention, but do not constitute a limitation of the present invention.

[0016] The endpoints of ranges and any values ​​disclosed herein are not limited to the exact range or value, and these ranges or values ​​should be understood to include approximations to these ranges.

[0017] Example 1 Anti-heat stress Chinese medicine composition

[0018] The anti-heat stress Chinese medicine composition was prepared and made into powder, and the components and contents were as follows.

[0019] Table 1 Anti-heat stress Chinese medicine compositions with different proportions

[0020]

[0021]

[0022] Example 2 Effect of the Anti-Heat Stress Chinese Medicine Composition on Heat Stress in Beef Cattle

[0023] Twenty adult hybrid Simmental cows with body weight (310.42±14.75) kg, similar age (1-2 years) and good body condition were selected and randomly divided into 5 groups, with 4 cows in each group, including heat stress (HS) group, low dose Baihu Tang flavored formula (LBH) group, high dose Baihu Tang flavored formula (HBH) group and physical cooling (PC) group.

[0024] The LBH group was supplemented with 250 g / d of the anti-heat stress Chinese medicine composition of Formula 1 in Example 1, the HBH group was supplemented with 500 g / d of the anti-heat stress Chinese medicine composition of Formula 1, and the PC group was cooled by fans. The experiment was conducted for a total of 20 days, including a 4-day pre-feeding period and a 16-day formal test period.

[0025] 2.1 Temperature and humidity records of cattle shed environment

[0026] The thermometer and hygrometer are set 1.5m above the center of the cowshed floor. The temperature and humidity of the cowshed are recorded at 08:00 and 14:00 every day, and the daily average temperature, humidity and temperature and humidity index (THI) of the cowshed are calculated; the calculation formula is: THI = td-(0.55-0.55RH)×(td-58); td = T×9 / 5+32. In the formula: td is Fahrenheit temperature (℉), T is Celsius temperature (℃), and RH is relative humidity (%).

[0027] When THI < 72, it means no heat stress; 72 ≤ THI ≤ 79, it means mild heat stress; 79 < THI ≤ 88, it means moderate heat stress; THI > 88, it means severe heat stress. According to the THI calculation formula, the THI during the test period was greater than 72, so the beef cattle were in a state of heat stress. The THI index of the cattle farm during the 20-day test is as follows: Figure 1 As shown in the figure, the THI of the cow sheds was greater than 72, with the highest reaching 84.01. This shows that the experimental cattle were in a state of heat stress during the experiment.

[0028] 2.2 Growth performance determination

[0029] During the experiment, the amount of TMR fed to the experimental cattle was recorded every day. The body weight (BW) of the beef cattle was measured on the 0th day and the last day of the experiment. The average daily feed intake (ADFI) and the average daily weight gain (ADG) were calculated. The results are shown in Table 2. It can be seen from Table 2 that the body weight gain and average daily weight gain of the LBH group were significantly higher than those of the HS group (P<0.01), and the final body weight, body weight gain and average daily weight gain of the HBH group were significantly higher than those of the HS group (P<0.05).

[0030] Table 2 Effect of anti-heat stress Chinese medicine composition on feed intake and weight gain of beef cattle (N=5)

[0031]

[0032]

[0033] Note: The data in the table are mean ± standard error. Different uppercase and lowercase letters in the same row indicate extremely significant differences (P<0.01) and significant differences (P<0.05), respectively, the same below.

[0034] 2.3 Measurement of rectal temperature and respiratory rate

[0035] The respiratory rate of the cattle was measured every 3 days by taking the number of chest and abdominal rises and falls per minute as the respiratory rate of the cattle. Similarly, the rectal temperature (RT) of the cattle was measured every 4 days using a thermometer.

[0036] The results of the respiratory rate of beef cattle are shown in Table 3. On the afternoon of the 13th day of the experiment, the respiratory rate of the LBH group was significantly lower than that of the HS group (P<0.05). On the afternoon of the 16th day of the experiment, the respiratory rate of the LBH group and the HBH group was significantly lower than that of the HS group (P<0.05). On the afternoon of the 19th day of the experiment, the respiratory rate of the LBH group, the HBH group and the PC group was significantly lower than that of the HS group (P<0.05).

[0037] Table 3 Effect of anti-heat stress Chinese medicine composition on the respiratory rate of beef cattle (times / min)

[0038]

[0039] The results of the rectal temperature of beef cattle are shown in Table 4. On the second day of the experiment, the rectal temperature of the HBH group was significantly lower than that of the HS group (P<0.01), and the rectal temperature of the LBH group was significantly lower than that of the HS group (P<0.05). On the 14th day of the experiment, the rectal temperature of the PC group was significantly lower than that of the HS group (P<0.01), and the rectal temperature of the LBH group was significantly lower than that of the HS group (P<0.05). On the 18th day of the experiment, the rectal temperatures of the LBH and PC groups were significantly lower than those of the HS group (P<0.01), and the rectal temperature of the HBH group was significantly lower than that of the HS group (P<0.05).

[0040] Table 4 Effect of anti-heat stress Chinese medicine composition on rectal temperature of beef cattle (℃)

[0041]

[0042] 2.4 Serum preparation and index determination

[0043] Blood was collected from the jugular vein on the 0th day and the last day of the experiment, 10 mL per cow, for the detection of physiological and biochemical indicators of beef cattle. The blood collected from the non-anticoagulant tube was allowed to stand for 30 minutes, centrifuged at 3000 r / min for 15 minutes to separate the plasma, and stored in a -20°C freezer. 2+ ), potassium ion (K + ), chloride ion (Cl - ), total antioxidant capacity (T-AOC), total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein (TP), creatine kinase (CK), lactate dehydrogenase (LDH), heat shock protein 70 (HSP70), malondialdehyde (MDA). The above indicators were determined according to the instructions of the kit and the data were read using a semi-automatic biochemical analyzer.

[0044] The ion indicators in serum are shown in Table 5. In the serum collected on day 0 of the experiment, K + , Ca 2+ and Cl - There was no significant difference in the concentration of K in the serum of the PC group. + Compared with the HS group, the Cl - The concentration was significantly higher than that of Cl in the PC group - concentration; Ca 2+ Although there was no significant difference among the groups, the serum Ca 2+ The content is the highest compared with other groups.

[0045] Table 5 Effects of anti-heat stress Chinese medicine composition on ion indexes of beef cattle

[0046]

[0047] The serum biochemical indices are shown in Table 6. In the serum collected on day 0 of the experiment, there were no significant differences in the levels of ALT, AST, CK and LDH; in the serum collected on day 20 of the experiment, the ALT in the LBH group and HBH group was significantly lower than that in the HS group (P<0.01), and the ALT in the PC group was significantly lower than that in the HS group (P<0.05); the AST content in the serum of the LBH group and HBH group was significantly lower than that in the HS group (P<0.05); the CK in the serum of the LBH group and PC group was significantly lower than that of the HS group (P<0.05); the LDH in the serum of the HS group was significantly higher than that of the other groups (P<0.01).

[0048] Table 6 Effects of anti-heat stress Chinese medicine composition on serum biochemical parameters of beef cattle

[0049]

[0050] The serum biochemical indices in serum are shown in Table 7. In the serum collected on day 0 of the experiment, there were no significant differences in the concentrations of T-SOD, T-AOC, MDA, GSP-Px and CAT. In the serum collected on day 20 of the experiment, T-SOD and T-AOC in the LBH group, HBH group and PC group were significantly higher than those in the HS group (P<0.01). The MDA in the PC group was significantly lower than that in the other groups (P<0.01). The GSP-Px in the PC group was significantly higher than that in the HS group (P<0.01), and the GSP-Px in the LBH group and HBH group was significantly higher than that in the HS group (P<0.05). The CAT content in the LBH group and PC group was significantly higher than that in the HS group (P<0.01), and the CAT content in the HBH group was significantly higher than that in the HS group (P<0.05).

[0051] Table 7 Effects of anti-heat stress Chinese medicine composition on antioxidant indexes of beef cattle

[0052]

[0053]

[0054] The serum biochemical indices in serum are shown in Table 8. In the serum collected on day 0 of the experiment, there were no significant differences in the concentrations of TP and HSP70. In the serum collected on day 20 of the experiment, TP in the LBH group, HBH group and PC group was significantly higher than that in the HS group (P<0.05), HSP70 in the LBH group and PC group was extremely significantly lower than that in the HS group (P<0.01), and HSP70 in the HBH group was significantly lower than that in the HS group (P<0.05).

[0055] Table 8 Effects of anti-heat stress Chinese medicine composition on total protein and heat shock protein HSP70 in beef cattle

[0056]

[0057] According to the above results, the anti-heat stress Chinese medicine composition has high safety and low toxicity. Adding the anti-heat stress Chinese medicine composition to the feed of beef cattle can improve its physiological and biochemical indicators under heat stress conditions, such as increasing the daily weight gain of beef cattle, reducing the respiratory rate of beef cattle, and alleviating a series of adverse effects such as liver damage and oxidative damage caused by heat stress on beef cattle. It shows that the anti-heat stress Chinese medicine composition can effectively alleviate the adverse effects on the growth and development of beef cattle under high temperature conditions in summer, and the best effect is achieved by adding 250g / d of the anti-heat stress Chinese medicine composition.

[0058] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention and do not constitute a limitation on the content of the present invention. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A Chinese medicine composition for resisting heat stress, characterized in that: The invention comprises the following components in parts by weight: 6-12 parts of Patchouli, 4-8 parts of Smilax glabra, 4-8 parts of Phragmites australis, 4-8 parts of Caulis Lonicera, 1-5 parts of Schizonepeta tenuifolia, 1-5 parts of Patrinia scabra, 1-5 parts of Imperata cylindrica, 1-5 parts of Lophatherum gracile, 1-5 parts of Gentiana scabra, 1-5 parts of Citrus reticulata, 1-5 parts of Gypsum, 1-5 parts of Anemarrhena asphodeloides, 1-5 parts of Humulus japonicus, and 1-5 parts of Acorus calamus.

2. The anti-heat stress Chinese medicine composition according to claim 1, characterized in that: The following components are used in parts by weight: Ingredients: Patchouli 9, Smilax glabra 6, Phragmites australis 6, Caulis Lonicera 6, Schizonepeta tenuifolia 3, Patrinia 3, Imperata cylindrica 3, Lophatherum gracile 3, Gentiana scabra 3, Citrus reticulata 3, Gypsum 3, Anemarrhena asphodeloides 3, Humulus japonicus 3, Acorus gramineus 3.

3. The anti-heat stress Chinese medicine composition according to claim 1, characterized in that: The anti-heat stress Chinese medicine composition is a powder.

4. Use of the heat stress resistant Chinese medicinal composition according to any one of claims 1 to 3 for preparing ruminant feed.

5. The use according to claim 4, characterized in that The ruminant is beef cattle.

6. A method for raising beef cattle, characterized in that: include: When the temperature and humidity index (THI) of the cowshed is above 72, 200-300 g of the anti-heat stress Chinese medicinal composition according to any one of claims 1 to 3 is added to the beef cattle feed every day.

7. The method according to claim 6, characterized in that When the temperature and humidity index (THI) of the cowshed is above 79, 250 g of the anti-heat stress Chinese medicinal composition is added to the beef cattle feed every day.

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