Blood-activating traditional Chinese medicine composition for relieving pain as well as preparation method and application of blood-activating traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a blood-activating traditional Chinese medicine composition for relieving pain and a preparation method and application thereof. The blood-activating traditional Chinese medicine composition consists of the following components in parts by weight: 10-20 parts of ligusticum wallichii; 1-5 parts of curcuma zedoary; 1-5 parts of dragon's blood; 15-30 parts of radix achyranthis bidentatae; 10 to 20 parts of licorice root; 10 to 20 parts of myrrh; 5-15 parts of Chinese angelica; 10 to 15 parts of radix astragali seu hedysari; 5-10 parts of eucommia ulmoides and 10-15 parts of radix paeoniae rubra; through reasonable compatibility, the obtained traditional Chinese medicine composition has good effects of relieving pain and treating osteoarthritis.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicine compositions, and in particular relates to a pain-relieving and blood-activating Chinese medicine composition, a preparation method and an application thereof. Background Art

[0002] Pain in tendons, bones and joints, soft tissue and muscle damage, as well as frozen shoulder, bone hyperplasia, traumatic injury, chronic strain, lumbar disc herniation, etc. can all cause pain. These pains have greatly affected people's daily lives and caused inconvenience to people's daily lives. The muscles and joints of the whole body are also invaded by the "six evils" of wind, cold, heat, dampness, dryness and fire, or trauma, chronic strain, which penetrate the skin into the bones and cause disease and form "six poisons", which are what we usually call "bone poison". "Bone poison" exists on the bones and periosteum for a long time to form "old poison". Old poison causes repeated congestion, edema, adhesion and contracture of soft tissues around bones and joints, resulting in local blood circulation disorders, producing a large amount of acidic substances to stimulate blood vessels and damage muscle tissue, which manifests as soreness, numbness, swelling, and even redness, swelling and deformation of muscles, tendons, bones and joints. In severe cases, it will cause damage to joint bones, joint deformation, and inability to take care of oneself. Pain in tendons and bones is an important clinical manifestation of rheumatic bone disease.

[0003] Arthritis is a common autoimmune disease with chronic inflammation of joint tissue as the main manifestation. The main manifestations of diseased joints are inflammatory cell infiltration, synovial hyperplasia, pannus formation and the resulting cartilage and bone damage. Arthritis belongs to the category of "arthritis" in traditional Chinese medicine. The "Inner Canon" believes that the deficiency of qi and blood in the body, the combination of wind, cold and dampness, invade the human body, flow into the tendons, bones and blood vessels, and throb in the joints, which is the main cause and pathogenesis of arthritis. Therefore, the evil of wind, cold, dampness and heat is only the external condition for the occurrence of this disease, while insufficient qi and blood and imbalance of Ying and Wei are the internal factors of this disease. The treatment of this disease should be based on the method of dispelling wind, dispersing cold and removing dampness, invigorating qi, activating blood circulation and unblocking collaterals. The occurrence of knee synovitis is mostly due to acute trauma of the knee joint, or aseptic inflammation of the synovium caused by chronic strain, bone hyperplasia and rheumatic factors. There are two main pathological changes: first, the synovial blood vessels in the affected area dilate and become congested, and plasma, red blood cells, and white blood cells extravasate, producing a large amount of exudate; second, the synovial cells actively proliferate, and the cell secretion is abnormally strong, producing a large amount of mucin, joint cavity effusion, increased joint pressure, obstruction of lymphatic system circulation, and accumulation of acidic metabolites in the joints. Such long-term retention can aggravate synovial inflammation and lead to a vicious cycle of the disease. In the later stage, the synovium thickens, adheres, and the articular cartilage atrophies, leading to knee joint dysfunction.

[0004] In modern medicine, drugs for treating orthopedic diseases are usually chemical drugs containing estrogen, which have serious side effects when used clinically. Traditional Chinese medicine is deeply favored by doctors and patients because of its unique efficacy and few side effects.

[0005] For example, Chinese patent CN105168978A discloses a traditional Chinese medicine composition for dispelling wind and cold, relaxing muscles and activating blood circulation, eliminating stasis and dispersing nodules to treat pain in muscles, bones and joints, which is composed of the following components in parts by weight: 150-180 parts of honey, 10-20 parts of beeswax, 180-220 parts of silkworm, 2-8 parts of soap needles, 3-6 parts of dragon clothes, 10-15 parts of frankincense, 10-15 parts of myrrh, 5-10 parts of cinnabar, 5-10 parts of realgar, 10-15 parts of earthworms, 10-15 parts of seahorses, 1 part of musk, 3-8 parts of gastrodia, 3-9 parts of notopterygium, 3-9 parts of angelica dahurica, 4-8 parts of cassia twig, 4-8 parts of Cyathula capitata, and 5-10 parts of Cistanche deserticola. The Chinese medicine composition and Chinese medicine rehabilitation pills provided by the invention adopt a variety of Chinese medicines with synergistic effects and are scientifically formulated. They have the effects of dispelling wind and cold, relaxing muscles and activating blood circulation, eliminating stasis and dispersing knots, and are used for wind-cold-dampness arthralgia that damages muscles and bones.

[0006] For another example, Chinese patent CN103100017A discloses an external Chinese herbal health product for relieving muscle and bone pain and joint pain. The weight proportions of various raw materials in the Chinese herbal health product are: 10-20 parts of star anise, 10-20 parts of chuanxiong, 10-20 parts of salvia miltiorrhiza, 10-20 parts of angelica dahurica, 10-20 parts of safflower, 10-20 parts of millettia reticulata, 10-20 parts of lycopodiella ciliata, 10-20 parts of galangal, 10-20 parts of knotweed, 10-20 parts of siler, 10-20 parts of asarum, 10-20 parts of drynaria, 10-20 parts of geranium, 10-20 parts of bletilla striata, 10-20 parts of spatholobi, 10-20 parts of myrrh, 10-20 parts of frankincense, 10-20 parts of dipsaccharum officinale, 10-20 parts of notopterygium wilfordii, and 10-20 parts of earthworm. This invention uses traditional Chinese medicine that has the effects of nourishing the kidneys and strengthening bones, promoting blood circulation and removing blood stasis, and has a certain therapeutic effect on joints. It can improve local arterial blood supply dysfunction, enhance local blood flow, promote the regeneration of trabeculae, reduce cystic changes, absorb sclerotic bones, repair cartilage areas, and promote blood circulation and recovery from muscle atrophy.

[0007] However, the existing traditional Chinese medicine compositions have many kinds of ingredients, and have the problem that the blood circulation effect is not significant and cannot meet the demand for pain relief. Summary of the invention

[0008] Based on the deficiencies in the prior art, the present invention aims to provide a pain-relieving and blood-activating Chinese medicine composition, and a preparation method and application thereof. During the implementation of the present invention, various Chinese medicine ingredients are reasonably controlled and reasonably matched to obtain a Chinese medicine composition with good effects of relieving pain and treating osteoarthritis.

[0009] In order to achieve the above object, the present invention adopts the following technical solution:

[0010] A pain-relieving and blood-activating Chinese medicinal composition comprises the following components by weight: 10-20 parts of chuanxiong, 1-5 parts of zedoaria, 1-5 parts of sanguisorba officinalis, 15-30 parts of achyranthes bidentata, 10-20 parts of liquorice, 10-20 parts of myrrh, 5-15 parts of angelica, 10-15 parts of astragalus, 5-10 parts of eucommia and 10-15 parts of red peony root.

[0011] Preferably, the blood-activating Chinese medicine composition consists of the following components by weight: 12-18 parts of Chuanxiong; 2-3 parts of Curcuma; 2-3 parts of Sanguisorba officinalis; 20-28 parts of Achyranthes bidentata; 8-15 parts of Licorice; 8-15 parts of Myrrh; 8-12 parts of Angelica sinensis; 12-15 parts of Astragalus; 6-9 parts of Eucommia ulmoides and 12-15 parts of Red Peony Root.

[0012] More preferably, the blood-activating Chinese medicine composition consists of the following components by weight: 14-16 parts of Chuanxiong; 2.5-3 parts of Curcuma; 2.5-3 parts of Sanguisorba officinalis; 22-26 parts of Achyranthes bidentata; 7-8 parts of Licorice; 10-12 parts of Myrrh; 8-12 parts of Angelica sinensis; 13-15 parts of Astragalus; 7-8 parts of Eucommia ulmoides and 14-15 parts of Red Peony Root.

[0013] As some preferred implementation schemes, the mass ratio of Chuanxiong, Myrrh, Licorice and Eucommia ulmoides is 1.5-2.5:1-2:1-2:1;

[0014] More preferably, the mass ratio of the Rhizoma Chuanxiong, Myrrha, Glycyrrhiza and Eucommia ulmoides is 2:2:1:1.

[0015] As other preferred embodiments, the mass ratio of Angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is 1:2-3:1-2:1-2;

[0016] More preferably, the mass ratio of Angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Red Peony Root is 1:2.5:1.5:1.5.

[0017] As a preferred embodiment, the blood-activating Chinese medicine composition is composed of the following components by weight: 16 parts of Chuanxiong; 2.5 parts of Curcuma; 2.5 parts of Sanguisorba officinalis; 25 parts of Achyranthes bidentata; 8 parts of Licorice; 12 parts of Myrrh; 10 parts of Angelica sinensis; 15 parts of Astragalus; 8 parts of Eucommia ulmoides and 15 parts of Red Peony Root.

[0018] The present invention also provides a method for preparing the above-mentioned blood-activating Chinese medicine composition, comprising the following steps: taking a prescribed amount of medicinal materials, adding water to decoct, filtering to obtain a decoction, and concentrating the decoction to a thick extract to obtain the blood-activating Chinese medicine composition.

[0019] As some preferred embodiments, the preparation method of the blood-activating Chinese medicine composition comprises the following steps: taking a formula amount of medicinal materials, adding water and boiling for 2-3 times, filtering, combining the decoctions and concentrating to a relative density of 1.20-1.48 (60°C) to obtain the blood-activating Chinese medicine composition.

[0020] As some preferred embodiments, the preparation method of the blood-activating Chinese medicine composition comprises the following steps: taking a formula amount of medicinal materials, adding 8-12 times of water and boiling for 2-3 hours, filtering to obtain decoction 1, then adding 6-10 times of water and boiling for 1-2 hours, filtering to obtain decoction 2, combining decoction 1 and decoction 2 and concentrating them to a thick extract with a relative density of 1.20-1.48 (60°C) to obtain the blood-activating Chinese medicine composition.

[0021] The present invention also provides application of the above-mentioned blood-activating Chinese medicinal composition in preparing medicine for treating knee osteoarthritis.

[0022] The compatibility principle of the present invention is:

[0023] The present invention uses Chuanxiong, Curcuma, Sanguisorba officinalis and Achyranthes bidentata as the main drugs. Chuanxiong: pungent in taste, warm in nature; enters the liver, gallbladder and pericardium meridians; activates blood circulation and removes blood stasis; promotes qi and relieves depression; dispels wind and relieves pain; Curcuma: pungent, bitter and warm; enters the liver and spleen meridians; promotes qi and removes blood, eliminates accumulation and relieves pain; the two are used together to enhance blood circulation and blood stasis, and strengthen qi and pain relief; Sanguisorba officinalis: neutral in nature; sweet and salty in taste; enters the heart and liver meridians; removes blood stasis and relieves pain, stops bleeding and promotes tissue regeneration; Achyranthes bidentata: bitter, sour and neutral; enters the liver and kidney meridians; nourishes the liver and kidney, strengthens tendons and bones, removes blood stasis and dredges the meridians, and guides blood downward; the two are used together to remove blood stasis and dredge the meridians, and adjust the circulation of qi and blood; the four drugs are used together to regulate the symptoms of osteoarthritis stasis and pain;

[0024] Licorice, myrrh and angelica are used as assistant drugs. Licorice: sweet, flat, non-toxic; tonifies the spleen and replenishes qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves pain; myrrh: bitter, flat, promotes blood circulation and relieves pain, reduces swelling and promotes tissue regeneration; angelica: sweet, pungent, warm; tonifies blood; promotes blood circulation; regulates menstruation and relieves pain; the combination of the three can strengthen qi and blood circulation, relieve pain, and help the main drug to replenish qi and promote blood circulation, disperse blood stasis and relieve pain;

[0025] Astragalus is used as an adjuvant. Astragalus is sweet and warm in nature. It enters the lung and spleen meridians. It can replenish qi and strengthen the exterior, promote diuresis and expel toxins, discharge pus, heal sores and promote tissue regeneration, and assist the main and auxiliary drugs in dispersing blood stasis and relieving pain.

[0026] Eucommia ulmoides and red peony root are used as guiding drugs. Eucommia ulmoides is sweet and warm in nature. It can nourish the liver and kidney, strengthen the tendons and bones, regulate the Chong and Ren meridians, consolidate the menstruation and stabilize the fetus. Red peony root is sweet and slightly cold. It enters the liver meridian. It can clear away heat and cool blood, disperse blood stasis and relieve pain. The two are used together to harmonize the various drugs to ensure the mild and lasting tonic effect and avoid the discomfort caused by excessive warmth.

[0027] Compared with the prior art, the beneficial effects of the present invention are:

[0028] (1) The present invention uses Chuanxiong, Curcuma, Sanguisorba officinalis and Achyranthes bidentata as main drugs, Licorice, Myrrh and Angelica sinensis as assistant drugs, Astragalus as adjuvant drug, Eucommia ulmoides and Paeonia lactiflora as guiding drugs, and the obtained active Chinese medicine composition has good effects of relieving pain and treating arthritis.

[0029] (2) The present invention improves the pain-relieving and arthritis-treating effects of the blood-activating Chinese medicine composition by controlling the mass ratio of Chuanxiong, Myrrh, Licorice and Eucommia to 1.5-2.5:1-2:1-2:1; and the mass ratio of Angelica, Achyranthes, Astragalus and Red Peony to 1:2-3:1-2:1-2. DETAILED DESCRIPTION

[0030] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0031] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0032] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. Various modifications and variations may be made to the specific embodiments of the present description without departing from the scope or spirit of the present invention, which will be apparent to those skilled in the art. Other embodiments obtained from the present description will be apparent to the technician. The present description and examples are exemplary only.

[0033] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0034] Example 1

[0035] A pain-relieving and blood-activating Chinese medicinal composition comprises the following components by weight: 10 parts of chuanxiong, 1 part of zedoaria, 1 part of sanguisorba officinalis, 15 parts of achyranthes bidentata, 10 parts of liquorice, 10 parts of myrrh, 5 parts of angelica, 10 parts of astragalus, 5 parts of eucommia and 10 parts of red peony root.

[0036] The preparation method of the above-mentioned blood-activating Chinese medicine composition is as follows: take the prescribed amount of medicinal materials, add 8 times the water and boil for 2 hours, filter to obtain decoction 1, then add 6 times the water and boil for 1 hour, filter to obtain decoction 2, combine decoction 1 and decoction 2 and concentrate to a thick extract with a relative density of 1.3 (60°C) to obtain the blood-activating Chinese medicine composition.

[0037] Example 2

[0038] A pain-relieving and blood-activating Chinese medicinal composition comprises the following components by weight: 20 parts of chuanxiong, 5 parts of zedoaria, 5 parts of sanguisorba officinalis, 30 parts of achyranthes bidentata, 20 parts of liquorice, 20 parts of myrrh, 15 parts of angelica sinensis, 15 parts of astragalus, 10 parts of eucommia bark and 15 parts of red peony root.

[0039] The preparation method of the above-mentioned blood-activating Chinese medicine composition is as follows: take the prescribed amount of medicinal materials, add 12 times the water and boil for 3 hours, filter to obtain decoction 1, then add 10 times the water and boil for 2 hours, filter to obtain decoction 2, combine decoction 1 and decoction 2 and concentrate to a thick extract with a relative density of 1.3 (60°C) to obtain the blood-activating Chinese medicine composition.

[0040] Example 3

[0041] A pain-relieving and blood-activating Chinese medicinal composition comprises the following components by weight: 16 parts of chuanxiong, 2.5 parts of zedoaria, 2.5 parts of sanguisorba officinalis, 25 parts of achyranthes bidentata, 8 parts of liquorice, 12 parts of myrrh, 10 parts of angelica, 15 parts of astragalus, 8 parts of eucommia and 15 parts of red peony root.

[0042] The preparation method of the above-mentioned blood-activating Chinese medicine composition is as follows: take the prescribed amount of medicinal materials, add 10 times the water and boil for 2.5 hours, filter to obtain decoction 1, then add 8 times the water and boil for 1.5 hours, filter to obtain decoction 2, combine decoction 1 and decoction 2 and concentrate to a thick extract with a relative density of 1.3 (60°C) to obtain the blood-activating Chinese medicine composition.

[0043] Comparative Example 1

[0044] The only difference from Example 3 is that Achyranthes bidentata is replaced by an equal weight portion of Astragalus membranaceus, and Sanguisorba officinalis is replaced by an equal weight portion of frankincense; the rest are the same.

[0045] Comparative Example 2

[0046] The only difference from Example 3 is that Curcuma is replaced by an equal amount of safflower, and Achyranthes is replaced by an equal amount of peach kernel; the rest are the same.

[0047] Comparative Example 3

[0048] The only difference from Example 3 is that Angelica sinensis is replaced by an equal weight portion of Angelica dahurica; Licorice is replaced by an equal weight portion of Centella asiatica; and the rest are the same.

[0049] Comparative Example 4

[0050] The only difference from Example 3 is that Angelica sinensis is replaced with Panax notoginseng in equal parts by weight; the rest are the same.

[0051] Comparative Example 5

[0052] The only difference from Example 3 is that the blood-activating Chinese medicine composition consists of the following components by weight: 16 parts of Chuanxiong; 2.5 parts of Curcuma; 2.5 parts of Sanguisorba officinalis; 25 parts of Astragalus; 8 parts of Licorice; 12 parts of Myrrh; 10 parts of Angelica sinensis; 15 parts of Achyranthes bidentata; 8 parts of Eucommia ulmoides and 15 parts of Paeonia lactiflora; the rest are the same.

[0053] Comparative Example 6

[0054] The only difference from Example 3 is that the blood-activating Chinese medicine composition consists of the following components by weight: 22 parts of Chuanxiong; 2.5 parts of Curcuma; 2.5 parts of Sanguisorba officinalis; 25 parts of Achyranthes bidentata; 4 parts of Licorice; 9 parts of Myrrh; 10 parts of Angelica sinensis; 15 parts of Astragalus; 15 parts of Eucommia ulmoides and 15 parts of Paeonia lactiflora; the rest are the same.

[0055] Comparative Example 7

[0056] The only difference from Example 3 is that the blood-activating Chinese medicine composition consists of the following components by weight: 16 parts of Chuanxiong; 2.5 parts of Curcuma; 2.5 parts of Sanguisorba officinalis; 20 parts of Achyranthes bidentata; 8 parts of Licorice; 12 parts of Myrrh; 6 parts of Angelica sinensis; 20 parts of Astragalus; 8 parts of Eucommia ulmoides and 19 parts of Red Peony Root; the rest are the same.

[0057] Experimental Example 1 Pain Relief Effect

[0058] 1. Experimental animals: 130 SPF male rats, 6-8 weeks old, weighing 180-220 g.

[0059] 2. Modeling: Rats were anesthetized with urethane, and after they lost consciousness, the right knee joint was locally prepared and then disinfected with iodine, and 3 mg / 50 μL sodium iodoacetate was injected into the rat knee joint cavity.

[0060] 3. Grouping: The rats were randomly divided into 12 groups: model control group, Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Shentong Zhuyu Decoction group; 10 rats in each group; another 10 rats were taken as the normal control group and injected with the same volume of physiological saline.

[0061] Shentong Zhuyu Decoction Group: add 10 times the amount of water to 3g of Gentiana macrophylla, 6g of Ligusticum chuanxiong, 9g of peach kernel, 9g of safflower, 6g of liquorice, 3g of Notopterygium wilfordii, 6g of myrrh, 9g of Angelica sinensis, 6g of Lingzhi (fried), 3g of Cyperus rotundus, 9g of Achyranthes bidentata, and 6g of Pheretima (soil removed), and boil for 2.5h, filter to obtain decoction 1, then add 8 times the amount of water and boil for 2h, filter to obtain decoction 2, combine decoction 1 and decoction 2, and concentrate them into a thick extract to obtain the Shentong Zhuyu Decoction Group.

[0062] 4. Administration: Inject 3 mg / 50 μL MIA and administer by gavage on the 10th day. The corresponding blood-activating Chinese medicine composition (prepared into liquid with normal saline in advance) 18 g / kg was administered by gavage in the embodiment and the comparative example, and the administration volume was 10 mL / kg. The normal control group and the model control group were given an equal amount of normal saline. The Shentong Zhuyu Decoction group was given a dose of 18 g / kg, and the administration volume was 10 mL / kg, once a day for 14 consecutive days.

[0063] 5. Measurement of mechanical pain threshold: After drug administration, standard nylon thread was used to stimulate the right hind paw of the rat. The number of nylon thread tested was gradually increased from the thinnest and softest until the rat's right hind leg was lifted. The number of nylon thread used was the mechanical pain threshold. The threshold standard was measured in grams (g).

[0064] 6. Measurement of thermal pain threshold: After drug administration, the thermal pain threshold was measured by irradiating the right hind paw of the rat with a laser heat source lamp. The intensity of the laser heat source was 20%. The irradiation time required to cause the rat to lift its right hind leg was the thermal pain threshold. The threshold standard was measured in seconds (s).

[0065] 7. SPSS 21.0 statistical software was used for analysis and processing. The data were expressed as (x±s), and the t-test was used for comparison between groups.

[0066] 8. Results Analysis

[0067] 8.1 Mechanical pain thresholds are shown in Table 1:

[0068] Table 1

[0069] Group Before administration (g) After administration (g) Normal control group 21.3±0.52 20.8±0.65 Model control group <![CDATA[10±1.64 ** ]]> <![CDATA[10.3±0.31 ** ]]> Example 1 10.3±1.22 <![CDATA[14.7±0.52 ## ]]> Example 2 10.5±1.34 <![CDATA[14.3±1.26 # ]]> Example 3 11.2±0.92 <![CDATA[14.8±1.24 ## ]]> Comparative Example 1 10.6±1.16 12.3±1.20 Comparative Example 2 10.8±1.25 13±1.68 Comparative Example 3 10.3±1.22 13.3±1.92 Comparative Example 4 11±1.31 <![CDATA[13.7±1.26 # <!-- 5 -->]]> Comparative Example 5 10.5±0.96 11.7±1.75 Comparative Example 6 10.1±1.85 12.6±0.86 Comparative Example 7 10.6±1.34 12.1±2.17 Shentongzhuyu decoction group 10.5±1.53 <![CDATA[14.1±1.43 # ]]>

[0070] Note: Compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0071] 8.2 The thermal pain threshold is shown in Table 2:

[0072] Table 2

[0073]

[0074]

[0075] Note: Compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0076] It can be seen from Table 1 and Table 2 that the mechanical pain threshold and thermal pain threshold of the blood-activating Chinese herbal composition of Examples 1-3 of the present invention are significantly different from those of the model group; compared with the Shentong Zhuyu Decoction group, the mechanical pain threshold and thermal pain threshold are greater, indicating that the blood-activating Chinese herbal composition of Examples 1-3 of the present invention has a better analgesic effect than the Shentong Zhuyu Decoction.

[0077] In comparative example 1, Achyranthes bidentata was replaced with an equal weight portion of Astragalus, and Blood Dracaena was replaced with an equal weight portion of Frankincense. In comparative example 2, Curcuma zedoaria was replaced with an equal weight portion of Carthamus tinctorius, and Achyranthes bidentata was replaced with an equal weight portion of Prunus persica. In comparative example 3, Angelica sinensis was replaced with an equal weight portion of Angelica dahurica; Licorice was replaced with an equal weight portion of Centella asiatica. Compared with the model group, the obtained blood-activating Chinese medicine composition had no significant difference in mechanical pain threshold and thermal pain threshold.

[0078] In Comparative Example 4, angelica was replaced with Panax notoginseng in equal parts by weight. The obtained blood-activating Chinese medicine composition had significant differences in mechanical pain threshold and thermal pain threshold compared with the model group, but the effect was not as good as that of Examples 1-3.

[0079] In Comparative Example 5, the weight proportions of Astragalus and Achyranthes were replaced with each other, and the obtained blood-activating Chinese medicine composition had no significant difference in mechanical pain threshold and thermal pain threshold compared with the model group;

[0080] In comparative example 6, the mass ratio of Chuanxiong, Myrrh, Licorice and Eucommia ulmoides is not in the range of 1.5-2.5:1-2:1-2:1; in comparative example 7, the mass ratio of Angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is not in the range of 1:2-3:1-2:1-2; compared with the model group, the mechanical sting threshold and thermal pain threshold of the obtained blood-activating Chinese medicine composition have no significant differences.

[0081] Experimental Example 2 Effect of Treating Arthritis

[0082] 1. Experimental animals: 130 SPF male SD rats, 6-8 weeks old, weighing 180-220 g.

[0083] 2. Modeling: After rats inhale isoflurane for about 5 seconds to achieve general anesthesia, 50 mg mL insulin is drawn into a sterile insulin syringe. -1 25 μL of sodium urate suspension was injected into the rat's right ankle joint at a 30° angle toward the inside of the ankle joint. The bulging of the contralateral side of the joint cavity indicated that the model was successfully established.

[0084] 3. Grouping: The rats with successful modeling were randomly divided into 12 groups: model control group, Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Shentong Zhuyu Decoction group; 10 rats in each group; another 10 rats were taken as the normal control group and injected with the same volume of physiological saline.

[0085] 4. Administration: The corresponding blood-activating Chinese medicine composition (prepared into liquid with physiological saline in advance) of 18 g / kg in the embodiment and comparative example was administered by gavage at a volume of 10 mL / kg, the normal control group and the model control group were given an equal amount of physiological saline, and the Shentong Zhuyu Decoction group was given a dose of 18 g / kg in a volume of 10 mL / kg, once a day for 14 consecutive days.

[0086] 5. Detection: After administration, the rats were killed by cervical dislocation, the knee joints of the model rats were surgically opened, the rat synovial fluid was collected, and the levels of IL-1β, IL-6 and TNF-α in the rat synovial fluid were determined by ELISA.

[0087] 6. SPSS 21.0 statistical software was used for analysis and processing. The data were expressed as (x±s), and the t-test was used for comparison between groups.

[0088] 7. The results are shown in Table 3:

[0089] Table 3

[0090] Group IL-1β (pg / mL) IL-6 (pg / mL) TNF-α(pg / mL) Normal control group 134.7±13.19 40.2±7.72 75.6±8.62 Model control group <![CDATA[258.6±9.97 ** ]]> <![CDATA[203.6±8.38 ** ]]> <![CDATA[198.5±25.31 ** ]]> Example 1 <![CDATA[161.5±8.22 ## ]]> <![CDATA[71±7.79 ## ]]> <![CDATA[93.2±6.82 ## ]]> Example 2 <![CDATA[164±11.77 ## ]]> <![CDATA[71.2±10.19 ## ]]> <![CDATA[93.9±8.52 ## ]]> Example 3 <![CDATA[160.2±8.95 ## ]]> <![CDATA[70.3±7.29 ## ]]> <![CDATA[92.5±7.38 ## ]]> Comparative Example 1 233.3±12.47 161.7±23.21 164±17.55 Comparative Example 2 230.7±13.02 154±25.67 160.5±22.81 Comparative Example 3 230±16.32 152.5±26.55 156.8±24.94 Comparative Example 4 <![CDATA[215.8±19.43 # ]]> <![CDATA[121.6±26.25 # ]]> <![CDATA[126.3±21.61 # ]]> Comparative Example 5 226.2±32.99 162.5±27.15 168.1±25.21 Comparative Example 6 237.5±34.9 175.6±28.12 181.3±27.68 Comparative Example 7 235.3±30.86 175.1±27.95 179.5±25.95 Shentongzhuyu decoction group <![CDATA[216.8±16.36 # ]]> <![CDATA[127.4±22.45 # ]]> <![CDATA[128.3±23.01 # ]]>

[0091] Note: Compared with the normal control group, ** P<0.01; compared with the model control group, ## P<0.01, # P<0.05.

[0092] As can be seen from Table 3, compared with the model group, the blood-activating Chinese medicine composition of Examples 1-3 of the present invention has significant differences in IL-1β, IL-6 and TNF-α; compared with the Shentong Zhuyu Decoction group, IL-1β, IL-6 and TNF-α are smaller, indicating that the blood-activating Chinese medicine composition of Examples 1-3 of the present invention is better in treating arthritis than Shentong Zhuyu Decoction.

[0093] In comparative example 1, Achyranthes bidentata was replaced by an equal weight portion of Astragalus, and Blood Dracaena was replaced by an equal weight portion of Frankincense. In comparative example 2, Curcuma zedoaria was replaced by an equal weight portion of Carthamus tinctorius, and Achyranthes bidentata was replaced by an equal weight portion of Prunus persica. In comparative example 3, Angelica sinensis was replaced by an equal weight portion of Angelica dahurica; Licorice was replaced by an equal weight portion of Centella asiatica. Compared with the model group, the obtained blood-activating Chinese medicine composition had no significant difference in IL-1β, IL-6 and TNF-α.

[0094] In Comparative Example 4, angelica was replaced with Panax notoginseng in equal parts by weight. The obtained blood-activating Chinese medicine composition showed significant differences in IL-1β, IL-6 and TNF-α compared with the model group, but the effect was not as good as that of Examples 1-3.

[0095] In Comparative Example 5, the weight proportions of Astragalus and Achyranthes were replaced with each other, and the obtained blood-activating Chinese medicine composition had no significant difference in IL-1β, IL-6 and TNF-α compared with the model group;

[0096] In comparative example 6, the mass ratio of Chuanxiong, Myrrh, Licorice and Eucommia ulmoides is not in the range of 1.5-2.5:1-2:1-2:1; in comparative example 7, the mass ratio of Angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is not in the range of 1:2-3:1-2:1-2; compared with the model group, the obtained blood-activating Chinese medicine composition has no significant differences in IL-1β, IL-6 and TNF-α.

[0097] The above embodiments are only used to help understand the method and core idea of ​​the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A pain-relieving and blood-activating Chinese medicinal composition, characterized in that: The invention is composed of the following components by weight: 10-20 parts of Chuanxiong, 1-5 parts of Curcuma, 1-5 parts of Sanguisorba officinalis, 15-30 parts of Achyranthes bidentata, 10-20 parts of Licorice, 10-20 parts of Myrrha, 5-15 parts of Angelica sinensis, 10-15 parts of Astragalus, 5-10 parts of Eucommia ulmoides and 10-15 parts of Red Peony Root.

2. The blood-activating Chinese medicinal composition according to claim 1, characterized in that: The blood-activating Chinese medicine composition consists of the following components by weight: 12-18 parts of Chuanxiong, 2-3 parts of Curcuma, 2-3 parts of Sanguisorba officinalis, 20-28 parts of Achyranthes bidentata, 8-15 parts of Licorice, 8-15 parts of Myrrh, 8-12 parts of Angelica sinensis, 12-15 parts of Astragalus, 6-9 parts of Eucommia ulmoides and 12-15 parts of Red Peony Root.

3. The blood-activating Chinese medicinal composition according to claim 2, characterized in that: The blood-activating Chinese medicine composition is composed of the following components by weight: 14-16 parts of Chuanxiong; 2.5-3 parts of Curcuma; 2.5-3 parts of Sanguisorba officinalis; 22-26 parts of Achyranthes bidentata; 7-8 parts of Licorice; 10-12 parts of Myrrh; 8-12 parts of Angelica sinensis; 13-15 parts of Astragalus; 7-8 parts of Eucommia ulmoides and 14-15 parts of Red Peony Root.

4. The blood-activating Chinese medicinal composition according to claim 3, characterized in that: The mass ratio of the chuanxiong, myrrh, liquorice and eucommia bark is 1.5-2.5:1-2:1-2:

1.

5. The blood-activating Chinese medicinal composition according to claim 4, characterized in that: The mass ratio of the chuanxiong, myrrh, liquorice and eucommia bark is 2:2:1:

1.

6. The blood-activating Chinese medicinal composition according to claim 5, characterized in that: The mass ratio of the angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is 1:2-3:1-2:1-2.

7. The blood-activating Chinese medicinal composition according to claim 6, characterized in that: The mass ratio of the angelica sinensis, Achyranthes bidentata, Astragalus membranaceus and Paeonia lactiflora is 1:2.5:1.5:1.

5.

8. The blood-activating Chinese medicinal composition according to claim 7, characterized in that: The blood-activating Chinese medicine composition is composed of the following components by weight: 16 parts of Chuanxiong, 2.5 parts of Curcuma, 2.5 parts of Sanguisorba officinalis, 25 parts of Achyranthes bidentata, 8 parts of Licorice, 12 parts of Myrrh, 10 parts of Angelica sinensis, 15 parts of Astragalus, 8 parts of Eucommia ulmoides and 15 parts of Red Peony Root.

9. The method for preparing the blood-activating Chinese medicinal composition according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: taking a prescribed amount of medicinal materials, adding water to boil, filtering to obtain a boiling liquid, and concentrating the boiling liquid to a thick extract to obtain a blood-activating Chinese medicine composition.

10. Use of the blood-activating Chinese medicinal composition according to any one of claims 1 to 8 in the preparation of a drug for treating knee osteoarthritis.

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