Method for improving sciatica based on low-intensity pulsed ultrasound

Abstract

The invention discloses a method for improving sciatica based on low-intensity pulsed ultrasound, and relates to the technical field of medical treatment. The low-intensity pulse ultrasound generates vibration force when passing through a cell culture or tissue, and the vibration force causes temperature rise, so that the blood flow is increased, and muscular pain is reduced; after low-intensity pulsed ultrasound treatment, the expression level of m2 related genes and anti-inflammatory factors is also remarkably increased, in addition, the number of neutrophils and M1ma bacteriophages in injured muscles is reduced in an early stage due to low-intensity pulsed ultrasound stimulation, M2 type macrophages are increased, and the sciatica can be effectively improved.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to a method for improving sciatic nerve pain based on low-intensity pulsed ultrasound. Background Art

[0002] Neuralgia is one of the common symptoms of neurology. It refers to pain felt without external stimulation. It is the pain directly caused by the pathology or disease of the somatic sensory system. It can be divided into peripheral neuropathy and central neuropathy. There are many causes of neuralgia, which may be related to inflammation, vascular disease, tumor, trauma, diabetes, cervical spondylosis, porphyria, polycythemia vera, poisoning, and mental factors.

[0003] Low-intensity pulsed ultrasound (LIPUS) is currently a non-surgical, diagnostic, therapeutic, and safe tool in the medical field. It has been previously reported that LIPUS can accelerate the regeneration of the sciatic nerve (SCN) after neurotomy. LIPUS also promotes spinal cord fusion by regulating the polarization of macrophages. At the same time, LIPUS does not have any side effects, such as harmful, carcinogenic, and thermal effects that may degrade living tissue. LIPUS has anti-inflammatory and regenerative abilities in many diseases, such as sialadenitis, skeletal muscle injury, and knee synovitis. Therefore, LIPUS may inhibit early NP due to its anti-inflammatory ability. To test these hypotheses, the effects of LIPUS exposure on NP in the partial sciatic nerve ligation (PSL) model were evaluated. At the same time, the macrophage phenotype in the SCN and its effect on PSL-induced glial activation and neuroinflammation were also examined. For this reason, we propose a method based on low-intensity pulsed ultrasound to improve sciatic pain. Summary of the invention

[0004] The purpose of the present invention is to solve the problems mentioned in the above background technology. The present invention provides a method for improving sciatic nerve pain based on low-intensity pulsed ultrasound.

[0005] In order to achieve the above-mentioned purpose, the present invention specifically adopts the following technical solutions:

[0006] A method for improving sciatic nerve pain based on low-intensity pulsed ultrasound, comprising the following steps:

[0007] Step 1: Experimental preparation: two groups of mice with the same vital signs were placed in a standard laboratory, defined as the experimental group and the control group, and provided with water and feed for breeding;

[0008] Step 2: Nerve ligation: Part of the sciatic nerve of the mice in the experimental group was ligated, while the mice in the control group were not treated;

[0009] Step 3: Low-intensity pulsed ultrasound treatment, placing the ligated nerve in low-intensity pulsed ultrasound for treatment;

[0010] Step 4: Behavioral testing: placing the treated experimental group and the control group in metal cages, using electronic devices to perform hypersensitivity reactions, and storing the corresponding records;

[0011] Step 5: Analyze statistics, calculate and compare the data after behavioral detection, and record the difference values.

[0012] Furthermore, the specific conditions of the standard laboratory in step 1 are room temperature of 26° C., and light is used to simulate day and night.

[0013] Furthermore, the experimental group and the control group in step 1 are both composed of two mice, one male and one female, aged 7-11 weeks.

[0014] Furthermore, the effective sound intensity of the low-intensity pulsed ultrasound treatment in step 3 is less than 60 mW / cm 2 And output ultrasonic waves in pulse form.

[0015] Furthermore, the exposure system used in the low-intensity pulsed ultrasound treatment in step 3 has a built-in cross-sectional area of ​​5 cm 2 The surface sensor of the ultrasonic head shows an effective radiation area of ​​4.1cm 2 The average value of beam bundle unevenness ratio is 3.6.

[0016] Furthermore, the duration of the low-intensity pulsed ultrasound treatment in step three is one week, and the treatment lasts 15 minutes every day.

[0017] Furthermore, the electronic device used for the behavioral detection in step 4 is a fully automatic ultra-sensitive detector.

[0018] Furthermore, the specific size of the metal cage in step 4 is 5*5*5mm.

[0019] The beneficial effects of the present invention are as follows:

[0020] In the present invention, low-intensity pulsed ultrasound generates vibration force when passing through cell culture or tissue, and the vibration force causes the temperature to rise, thereby increasing blood flow, reducing muscle pain, etc.; after low-intensity pulsed ultrasound treatment, the expression levels of m2-related genes and anti-inflammatory factors also increase significantly. In addition, low-intensity pulsed ultrasound stimulation leads to an early decrease in the number of neutrophils and M1 ma phages in damaged muscles and an increase in M2 macrophages, which can effectively improve sciatic pain. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a work flow chart of the present invention. DETAILED DESCRIPTION

[0022] To make the purpose, technical solution and advantages of the embodiments of the present invention more clear, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0023] See also Figure 1 The present invention provides a method for improving sciatica pain based on low-intensity pulsed ultrasound, comprising the following steps:

[0024] Step 1: Experimental preparation: two groups of mice with the same vital signs were placed in a standard laboratory, defined as the experimental group and the control group, and provided with water and feed for breeding;

[0025] Step 2: Nerve ligation: Part of the sciatic nerve of the mice in the experimental group was ligated, while the mice in the control group were not treated;

[0026] Step 3: Low-intensity pulsed ultrasound treatment, placing the ligated nerve in low-intensity pulsed ultrasound for treatment;

[0027] Step 4: Behavioral testing: placing the treated experimental group and the control group in metal cages, using electronic devices to perform hypersensitivity reactions, and storing the corresponding records;

[0028] Step 5: Analyze statistics, calculate and compare the data after behavioral detection, and record the difference values.

[0029] In this embodiment, preferably, the specific conditions of the standard laboratory in step 1 are room temperature of 26° C., and light is used to simulate day and night.

[0030] In this embodiment, preferably, the test group and the control group in step 1 are both composed of two mice, one male and one female, aged 7-11 weeks.

[0031] In this embodiment, preferably, the effective sound intensity of the low-intensity pulsed ultrasound treatment in step 3 is less than 60 mW / cm 2 And output ultrasonic waves in pulse form.

[0032] In this embodiment, preferably, the exposure system used in the low-intensity pulsed ultrasound treatment in step 3 has a built-in cross-sectional area of ​​5 cm 2 The surface sensor of the ultrasonic head shows an effective radiation area of ​​4.1cm 2 The average value of beam bundle unevenness ratio is 3.6.

[0033] In this embodiment, preferably, the low-intensity pulsed ultrasound treatment in step three lasts for one week, with 15 minutes of treatment per day.

[0034] In this embodiment, preferably, the electronic device selected for the behavior detection in step 4 is a fully automatic ultra-sensitive detector.

[0035] In this embodiment, preferably, the specific size of the metal cage in step 4 is 5*5*5mm.

[0036] The working principle and use process of the present invention:

[0037] Step 1: Experimental preparation: two groups of mice with consistent vital signs are placed in a standard laboratory, defined as the experimental group and the control group respectively, and provided with water and feed for breeding. The specific conditions of the standard laboratory are room temperature of 26°C, and light is used to simulate day and night. Both the experimental group and the control group use two male and female mice aged 7-11 weeks. The standard laboratory can simulate natural conditions and avoid the influence of external conditions on the actual treatment effect.

[0038] Step 2: Nerve ligation: Part of the sciatic nerves of the mice in the test group were ligated, while the mice in the control group were not treated, so as to facilitate subsequent intuitive comparison and directly understand the actual treatment effect.

[0039] Step three: low-intensity pulsed ultrasound therapy. The ligated nerve is placed in low-intensity pulsed ultrasound for treatment. The effective sound intensity of low-intensity pulsed ultrasound therapy is less than 60mW / cm2, and the ultrasound is output in pulse form. The exposure system used in low-intensity pulsed ultrasound therapy has a built-in surface sensor with a cross-section of 5cm2. The ultrasound head shows an effective radiation area of ​​4.1cm2, and the average beam unevenness ratio is 3.6. The duration of low-intensity pulsed ultrasound therapy is one week, and the treatment lasts 15 minutes a day. Ultrasound waves generate vibration force when passing through cell cultures or tissues. The vibration force causes the temperature to rise, thereby increasing blood flow and reducing muscle pain.

[0040] Step 4: Behavioral detection. Place the experimental group and the control group that have completed the treatment in a metal cage, use an electronic device to perform a hypersensitivity reaction, and store the corresponding records. The selected electronic device is a fully automatic hypersensitivity detector. The specific size of the metal cage is 5*5*5mm. The fully automatic hypersensitivity detector has high detection accuracy, fast detection speed, simple operation, accurate and stable results, and a high degree of automation. It can ensure the accuracy of the detection. The metal cage can prevent mice from escaping and affecting the detection.

[0041] Step 5: Analyze and count the data after the behavior detection, calculate and compare them, record the difference values, and store the test difference values ​​in a mobile hard disk or cloud database for easy retrieval and use at any time.

[0042] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for improving sciatic nerve pain based on low-intensity pulsed ultrasound, characterized in that: The steps include: Step 1: Experimental preparation: two groups of mice with the same vital signs were placed in a standard laboratory, defined as the experimental group and the control group, and provided with water and feed for breeding; Step 2: Nerve ligation: Part of the sciatic nerve of the mice in the experimental group was ligated, while the mice in the control group were not treated; Step 3: Low-intensity pulsed ultrasound treatment, placing the ligated nerve in low-intensity pulsed ultrasound for treatment; Step 4: Behavioral testing: placing the treated experimental group and the control group in metal cages, using electronic devices to perform hypersensitivity reactions, and storing the corresponding records; Step 5: Analyze statistics, calculate and compare the data after behavioral detection, and record the difference values.

2. A method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: The specific conditions of the standard laboratory in step 1 are room temperature of 26° C., and light is used to simulate day and night.

3. The method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: In the step 1, the test group and the control group are both composed of two mice, one male and one female, aged 7-11 weeks.

4. The method for improving sciatic nerve pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: In step 3, the effective sound intensity of low-intensity pulsed ultrasound treatment is less than 60 mW / cm 2 And output ultrasonic waves in pulse form.

5. The method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: The exposure system used in the low-intensity pulsed ultrasound treatment in step 3 has a built-in cross-sectional area of ​​5 cm 2 The surface sensor of the ultrasonic head shows an effective radiation area of ​​4.1cm 2 The average value of beam bundle unevenness ratio is 3.

6.

6. The method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: The duration of the low-intensity pulsed ultrasound treatment in step 3 is one week, with 15 minutes of treatment per day.

7. The method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: The electronic device used for the behavioral detection in step 4 is a fully automatic ultra-sensitive detector.

8. The method for improving sciatica pain based on low-intensity pulsed ultrasound according to claim 1, characterized in that: The specific size of the metal cage in step 4 is 5*5*5mm.

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