Phyllostachys edulis pesvp gene, encoded protein and application thereof

By cloning the PeSVP gene of moso bamboo and constructing an overexpression vector, and transforming it into Arabidopsis thaliana, the flowering time of moso bamboo was successfully delayed and the number of rosette leaves was increased. This revealed the negative regulatory role of the PeSVP gene in regulating plant flowering, and the expression level of the FT gene was downregulated.

CN119932040BActive Publication Date: 2026-06-26NANJING FORESTRY UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING FORESTRY UNIV
Filing Date
2025-01-10
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Bamboo requires a long period of nutrient storage to flower, sometimes up to a hundred years. After flowering, the trees die, causing huge losses to production. Current technology lacks effective flowering regulation genes and mechanisms.

Method used

The PeSVP gene of moso bamboo was cloned, an overexpression vector was constructed and transformed into Arabidopsis thaliana, and transgenic Arabidopsis thaliana lines with delayed flowering time were obtained through breeding and screening to regulate the flowering time and number of rosette leaves of the plants.

Benefits of technology

The study successfully delayed the flowering time of moso bamboo and increased the number of rosette leaves, revealing that the PeSVP gene negatively regulates plant flowering and significantly downregulated the expression of the FT gene, thus achieving effective regulation of flowering time.

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Abstract

The application discloses a Phyllostachys edulis PeSVP gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the PeSVP gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs a PeSVP gene overexpression vector of Phyllostachys edulis, transforms the constructed PeSVP gene overexpression vector of Phyllostachys edulis into Arabidopsis thaliana, and cultivates, screens and obtains a transgenic Arabidopsis thaliana strain with delayed flowering time.The application discloses the function of the PeSVP gene for the first time, the transgenic Arabidopsis thaliana plant has delayed flowering time after the PeSVP gene is transformed into the Arabidopsis thaliana plant, shows an obvious late-flowering phenotype, and the number of rosette leaves is increased;the expression amount of a downstream regulation gene FT is significantly reduced.The application can be used in molecular breeding of plant flowering period regulation, and has important significance for cultivating new plant germplasm with higher biological yield.
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