High-pressure immunohistochemical control method, device, equipment and medium

Abstract

The invention provides a control method, device and equipment for high-pressure immunohistochemistry and a medium. The control method comprises the following steps: controlling a first mechanical arm to sequentially add N reagents on an incubation assembly in a workbench; controlling a second mechanical arm to adsorb and transfer the sample carrier to an incubation assembly after the reagent is added each time, covering each reagent with the sample carrier in an inverted buckling manner, and sequentially performing incubation until N times of incubation are completed to obtain a completely incubated sample carrier; wherein N is a positive integer. By integrating the workbench and the mechanical arm capable of performing three-dimensional motion, automatic transfer of a sample carrier between processing stations is realized, so that the requirement of manual operation is reduced, the processing speed and accuracy are greatly improved, the automation level and efficiency of an experiment are effectively improved, the accuracy and reliability of an experiment result are ensured, and the working efficiency is improved. In addition, an inverted covering mode is adopted, so that reagent loss can be avoided, evaporation can be reduced, reaction of non-antigen substances and reagents in the incubation process can be reduced, and non-specific reaction can be reduced.

Description

Technical Field

[0001] The invention relates to the field of biological reaction automation, and in particular to a control method, device, equipment and medium for high-voltage immunohistochemistry. Background Art

[0002] In the field of biotechnology, immunohistochemistry is used to study the expression of specific proteins in cell or tissue samples. This technology is widely used in medical diagnosis, drug development, and basic scientific research. In immunohistochemistry experiments, the high-pressure heat repair step is a key operation, which helps to increase the exposure of antigens and enhance the sensitivity of immunohistochemical reactions.

[0003] However, existing equipment often requires operators to manually load and unload sample carriers, which is not only time-consuming and laborious, but also complicated and inefficient to operate and easily leads to instability of experimental results. In addition, due to the subjective factors of operators, there are certain risks in the handling of sample carriers, which may cause damage or contamination of experimental samples.

[0004] Therefore, there is an urgent need for a control method, device, equipment and medium for high-pressure immunohistochemistry to improve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide a control method, device, equipment and medium for high-voltage immunohistochemistry, which can effectively improve the automation level and efficiency of the experiment and ensure the accuracy and reliability of the experimental results.

[0006] In a first aspect, the present invention provides a method for controlling high-pressure immunohistochemistry, comprising: Control the first robotic arm to sequentially add N reagents to the incubation component on the workbench; After each addition of reagent, the second robot arm is controlled to transfer the sample carrier to the incubation component by adsorption, and cover each reagent in an inverted manner for incubation in sequence, until a completely incubated sample carrier is obtained after N incubations; Wherein, N is a positive integer.

[0007] The beneficial effects of the method of the invention are as follows: by controlling the first robotic arm to sequentially add N kinds of reagents to the incubation component in the workbench; after each addition of the reagent, controlling the second robotic arm to transfer the sample carrier to the incubation component by adsorption, and covering each reagent in an inverted manner for sequential incubation, until a fully incubated sample carrier is obtained after N incubations; wherein N is a positive integer. By integrating a workbench and a robotic arm capable of three-dimensional movement, the automatic transfer of sample carriers between processing stations is realized, which not only reduces the need for manual operation, but also greatly improves the processing speed and accuracy, effectively improves the automation level and efficiency of the experiment, and ensures the accuracy and reliability of the experimental results. Compared with the prior art that sets the sample carrier with the front side facing up, the present invention adopts an inverted covering method, which can not only avoid the loss of reagents and reduce evaporation, but also can simultaneously reduce the reaction between non-antigenic substances and reagents during the incubation process, reduce nonspecific reactions, reduce noise, and improve sensitivity.

[0008] Optionally, the N reagents are one or more combinations of a blocking agent, a primary antibody reagent, an amplifier, a secondary antibody reagent, a substrate and a staining agent.

[0009] Optionally, when the value of N is five, controlling the first robotic arm to sequentially add N reagents to the incubation component on the workbench is specifically as follows: The first robot arm is controlled to sequentially add a blocking agent, a primary antibody reagent, an amplifier, a secondary antibody reagent and a substrate to the incubation component.

[0010] Optionally, after each addition of the reagent, the second robotic arm is controlled to transfer the sample carrier to the incubation component by adsorption, and cover each reagent in an inverted manner for incubation in sequence until the fully incubated sample carrier is obtained after N incubations. Specifically: After adding the blocking agent, controlling the second robot arm to transfer the first sample carrier to the incubation component by adsorption, and covering the first sample carrier in an inverted manner on the blocking agent for the first incubation to obtain a second sample carrier; After adding the primary antibody reagent, controlling the second robot arm to adsorb and transfer the second sample carrier to the incubation component, and covering the first antibody reagent in an inverted manner for a second incubation to obtain a third sample carrier; After adding the amplifying agent, controlling the second mechanical arm to transfer the third sample carrier to the incubation component by adsorption, and covering the amplifying agent in an inverted manner for a third incubation to obtain a fourth sample carrier; After adding the secondary antibody reagent, the second robot arm is controlled to transfer the fourth sample carrier to the incubation component by adsorption, and the fourth sample carrier is covered on the secondary antibody reagent in an inverted manner for a fourth incubation to obtain a fifth sample carrier; After adding the substrate, the second robot arm is controlled to transfer the fifth sample carrier to the incubation component by adsorption, and covers the fifth sample carrier on the substrate in an inverted manner for incubation for the fifth time to obtain a completely incubated sample carrier.

[0011] Optionally, the sample carrier is washed after the first incubation, the second incubation, the third incubation, and the fourth incubation.

[0012] Optionally, before controlling the first robot arm to sequentially add N reagents to the incubation component on the workbench, the method further includes: A plurality of sample carriers carrying samples are dewaxed and thermally repaired in a thermal repair reaction device to obtain treated sample carriers.

[0013] Optionally, also include: The third robot arm is controlled to place all sample carriers in the thermal repair reaction device on the transfer module for temporary storage, so as to transfer the sample carriers in the transfer module to the incubation component in batches.

[0014] Optionally, also include: During the adsorption and transfer, the identification information on the sample carrier is scanned and the sample carrier information is recorded.

[0015] In a second aspect, the present invention provides a device, which includes modules / units for executing any possible design method of the first aspect. These modules / units can be implemented by hardware, or by hardware executing corresponding software implementations.

[0016] In a third aspect, the present invention provides an electronic device, comprising a memory and a processor, wherein the memory stores a program that can be run on the processor, and when the program is executed by the processor, the electronic device implements a method for executing any possible design of any of the above aspects.

[0017] In a fourth aspect, the present invention provides a readable storage medium, wherein the readable storage medium stores a program, and when the program is executed, it implements any possible design method of any of the above aspects.

[0018] For the beneficial effects of the second to fourth aspects, reference may be made to the description of the first aspect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic flow chart of a control method for high-pressure immunohistochemistry provided by an embodiment of the present invention; Figure 2 A schematic diagram of the structure of a control device for high-voltage immunohistochemistry provided by an embodiment of the present invention; Figure 3 A schematic diagram of the structure of an electronic device provided by an embodiment of the present invention; Figure 4A high-voltage immunohistochemistry device used in a high-voltage immunohistochemistry control method provided in an embodiment of the present invention.

[0020] Reference numerals

[0021] 1. Workbench; 2. Incubation component; 3. First robotic arm; 4. Second robotic arm; 5. Third robotic arm; 6. Thermal repair reaction device; 7. Transfer module. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should be understood by people with general skills in the field to which the present invention belongs. "Including" and similar words used in this article mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects.

[0023] In view of the inconvenience of current high-pressure immunohistochemistry, the control method, device, equipment and medium of high-pressure immunohistochemistry provided by the present invention can improve the automation level and efficiency of the experiment, and can also ensure the accuracy and reliability of the experimental results. The technical scheme in the embodiment of the present invention is described below in conjunction with the drawings in the embodiment of the present invention. Among them, in the description of the embodiment of the present invention, the terms used in the following embodiments are only for the purpose of describing specific embodiments, and are not intended to be used as limitations on the present invention. As used in the specification of the present invention and the appended claims, the singular expressions "a", "said", "above", "the" and "this" are intended to also include expressions such as "one or more", unless there is a clear contrary indication in the context. It should also be understood that in the following embodiments of the present invention, "at least one", "one or more" refers to one or more than two (including two). The term "and / or" is used to describe the association relationship of associated objects, indicating that three relationships can exist; for example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone, where A and B can be singular or plural. The character " / " generally indicates that the objects associated before and after are in an "or" relationship.

[0024] References to "one embodiment" or "some embodiments" described in this specification mean that one or more embodiments of the present invention include specific features, structures or characteristics described in conjunction with the embodiment. Thus, the statements "in one embodiment", "in some embodiments", "in some other embodiments", and "in some other embodiments" that appear in different places in this specification do not necessarily refer to the same embodiment, but mean "one or more but not all embodiments", unless otherwise specifically emphasized in other ways. The terms "including", "comprising", "having" and their variations all mean "including but not limited to", unless otherwise specifically emphasized in other ways. The term "connected" includes direct and indirect connections, unless otherwise stated. "First" and "second" are used for descriptive purposes only and are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated.

[0025] In the embodiments of the present invention, "exemplarily" or "for example" is used to indicate an example, illustration or description. Any embodiment or design described as "exemplarily" or "for example" in the embodiments of the present invention should not be interpreted as being more preferred or more advantageous than other embodiments or designs. Specifically, the use of "exemplarily" or "for example" is intended to present the relevant concepts in a specific way.

[0026] like Figure 1 and 4 As shown, the present invention provides a control method for high-pressure immunohistochemistry, comprising: S101, controlling the first robot arm 3 to sequentially add N reagents to the incubation component 2 in the workbench 1.

[0027] In some embodiments, the N reagents are one or more combinations of a blocking agent, a primary antibody reagent, an amplifier, a secondary antibody reagent, a substrate, and a staining agent.

[0028] In some specific embodiments, when the value of N is five, controlling the first robot arm 3 to sequentially add N reagents to the incubation component 2 in the workbench 1 specifically includes: controlling the first robot arm 3 to sequentially add a blocking agent, a primary antibody reagent, an amplifier, a secondary antibody reagent, and a substrate to the incubation component 2. In other specific embodiments, the value of N is six, and after adding the substrate, the first robot arm 3 is controlled to add a stain (such as hematoxylin) to the sample carrier after complete incubation.

[0029] In some embodiments, before controlling the first robotic arm 3 to sequentially add N reagents to the incubation component 2 in the workbench 1, the process also includes: dewaxing and thermally repairing a plurality of sample carriers carrying samples in a thermal repair reaction device 6 to obtain treated sample carriers (such as glass slides).

[0030] In some specific embodiments, it also includes: controlling the third robot arm 5 to place all sample carriers in the thermal repair reaction device 6 on the transfer module 7 for temporary storage, so as to transfer the sample carriers in the transfer module 7 to the incubation component 2 in batches.

[0031] S102, after each reagent is added, the second robot arm 4 is controlled to transfer the sample carrier to the incubation component 2 by absorption, and cover each reagent in an inverted manner for incubation in sequence, until a fully incubated sample carrier is obtained after N incubations; wherein N is a positive integer. In the prior art, during the addition of reagents and incubation, the front side (the side with tissue or other samples) of the sample carrier (such as a glass slide) is set upward, and at most only a solid cover film is used, which easily causes the risk of reagent evaporation or direct loss from the sample carrier (such as a glass slide). After the reagent is lost, the tissue is directly exposed to the air and cannot be moisturized and dries up, which easily causes the failure of the experiment. The present invention adopts an inverted covering method, which can not only avoid the loss of reagents and reduce evaporation, but also reduce the reaction between non-antigen substances and reagents during the incubation process, reduce nonspecific reactions, reduce noise, and improve sensitivity.

[0032] In some embodiments, after each addition of a reagent, the second robot arm 4 is controlled to transfer the sample carrier to the incubation component 2 by absorption, and covers each reagent in an upside-down manner for incubation in sequence, until a fully incubated sample carrier is obtained after N incubations are completed. Specifically, after adding a blocking agent, the second robot arm 4 is controlled to transfer the first sample carrier to the incubation component 2 by absorption, and covers the blocking agent in an upside-down manner for the first incubation to obtain a second sample carrier; after adding a primary antibody reagent, the second robot arm 4 is controlled to transfer the second sample carrier to the incubation component 2 by absorption, and covers the primary antibody reagent in an upside-down manner for the second incubation incubate the sample carrier to obtain a third sample carrier; after adding the amplifier, control the second robot arm 4 to transfer the third sample carrier to the incubation component 2 by absorption, and cover the amplifier with the amplifier in an upside-down manner for the third incubation to obtain a fourth sample carrier; after adding the secondary antibody reagent, control the second robot arm 4 to transfer the fourth sample carrier to the incubation component 2 by absorption, and cover the secondary antibody reagent with the upside-down manner for the fourth incubation to obtain a fifth sample carrier; after adding the substrate, control the second robot arm 4 to transfer the fifth sample carrier to the incubation component 2 by absorption, and cover the substrate with the upside-down manner for the fifth incubation to obtain a fully incubated sample carrier.

[0033] In some embodiments, the sample carrier is washed after the first incubation, after the second incubation, after the third incubation, and after the fourth incubation.

[0034] In some embodiments, the method further includes: scanning identification information on the sample carrier during adsorption and transfer, and recording the sample carrier information.

[0035] For ease of understanding, this embodiment further describes the specific implementation process of the above method in combination with a specific application scenario system. In this embodiment, the value of N is five, and the method specifically includes the following steps: Step a, dewaxing and thermally repairing several sample carriers carrying samples in the thermal repair reaction device 6 to obtain the treated sample carriers. The user starts the device through the touch screen interface and selects the preset dewaxing thermal repair program. The control unit starts the heating element to preheat the reactor to the target temperature, for example, setting the temperature between 65°C and 75°C. The thermal repair reaction device 6 also includes an air pressure detection module for detecting and judging whether the suction cup adsorbs the sample carrier. In this way, by real-time monitoring of the air pressure state of the suction cup, the air pressure detection module can accurately determine whether the sample carrier is successfully adsorbed, ensuring the reliability of the sample carrier transfer and processing process, and avoiding experimental failure or repeated work caused by the suction cup failing to correctly adsorb the sample carrier. The air pressure detection module can automatically detect the functional status of the suction cup system, such as whether the suction cup is leaking or whether the pipeline is blocked, reducing the complexity and frequency of equipment maintenance, while preventing possible operational errors in advance. Ensuring that the sample carrier can be accurately adsorbed every time helps to maintain the consistency and repeatability of the experiment, which is very critical for the accurate interpretation and subsequent analysis of experimental data. By reducing the loss of sample carriers and repeated experiments caused by operating errors, the air pressure detection module helps save experimental materials and valuable time, improves overall experimental efficiency, and further improves the automation level of the entire equipment, making the equipment operation more intelligent and automatic, and reducing the dependence on operator skills. The air pressure detection module not only improves the safety and reliability of the experiment, but also significantly improves the performance of the entire equipment and the effectiveness of the experiment by improving the accuracy and automation of the operation.

[0036] Step b: controlling the third robot arm 5 to place all sample carriers in the thermal repair reaction device 6 on the transfer module 7 for temporary storage, so as to transfer the sample carriers in the transfer module 7 to the incubation component 2 in batches.

[0037] Step c, control the liquid adding device in the first robot arm 3 component to add blocking agent to the incubation component 2 in advance; control the second suction cup of the second robot arm 4 component to adsorb and transfer the sample carrier on the transfer module 7 to the incubation component 2 in an inverted manner to cover it on the blocking agent for the first incubation, and then clean the sample carrier for the first time.

[0038] Step d, control the liquid adding device in the first robotic arm 3 component to add a primary antibody reagent to the clean position of the incubation component 2 in advance; control the second suction cup of the second robotic arm 4 component to adsorb and transfer the sample carrier after the first cleaning, cover it in an inverted manner on the primary antibody reagent for a second incubation, and then clean the sample carrier for a second time.

[0039] Step e, control the liquid adding device in the first robotic arm 3 component to add the amplifier to the clean position of the incubation component 2 in advance; control the second suction cup of the second robotic arm 4 component to adsorb and transfer the sample carrier after the first cleaning, and cover it on the amplifier in an inverted manner for the third incubation; and clean the sample carrier for the third time.

[0040] Step f, control the liquid adding device in the first robotic arm 3 component, add the secondary antibody reagent to the clean position of the incubation component 2 in advance, control the second suction cup of the second robotic arm 4 component to adsorb and transfer the sample carrier after the first cleaning, and cover it in an inverted manner on the secondary antibody reagent for the fourth incubation; clean the sample carrier for the fourth time.

[0041] Step g, control the liquid adding device in the first robotic arm 3 component to add the substrate to the clean position of the incubation component 2 in advance; control the second suction cup of the second robotic arm 4 component to adsorb and transfer the sample carrier after the first cleaning, and cover it on the substrate in an inverted manner for the fifth incubation.

[0042] During the adsorption and transfer, the QR code on the sample carrier is scanned to obtain the identification information and record the sample carrier information. It is worth noting that the identification information can also be stored through an RFID radio frequency tag.

[0043] The advantage of this embodiment is that by integrating the thermal repair reaction device 6, the incubation component 2 and the mechanical arm on a workbench 1 that can perform three-dimensional movement, the sample processing, incubation and cleaning process can be automatically completed, significantly improving the experimental efficiency and sample processing speed. The mechanical arm automatically transfers the sample carrier and distributes the reagent, reducing the manual operation during the experiment, thereby reducing possible operating errors and improving the accuracy and repeatability of the experimental results. Automation, the user only needs to set the relevant parameters to automatically complete the entire experimental process, reducing the complexity of the operation.

[0044] Moreover, the robotic arm can precisely control the position of the sample carrier in space with the three-dimensional motion platform, ensuring that the sample carrier is accurately placed during thermal repair, dewaxing and other processes, reducing operational errors. The automatic sample carrier transfer module improves the repeatability and accuracy of the experiment by precisely controlling the movement and positioning of the sample carrier, which is particularly important for scientific research and clinical diagnosis. The application of the automatic sample carrier transfer module enables the entire experimental process to be fully automated without manual intervention, significantly improving the overall efficiency of the experiment and the operating efficiency of the laboratory. In addition, due to the reduction of manual operation, the sample carrier reduces contact with the outside world during the transfer process, effectively reducing the risk of cross contamination. At the same time, the automatic sample carrier transfer module is simple in design, easy to carry out daily maintenance and inspection, and reduces the maintenance cost and complexity of the equipment. These improvements comprehensively enhance the functionality and practicality of the equipment, making the device more efficient and reliable in biochemical analysis fields such as immunohistochemistry.

[0045] like Figure 2 As shown, based on the above-mentioned high-pressure immunohistochemistry control method, the present invention provides a high-pressure immunohistochemistry control device, including: a liquid addition control module 201, used to control the first robot arm 3 to add N reagents to the incubation component 2 in the workbench 1 in sequence; a transfer control module 202, used to control the second robot arm 4 to adsorb and transfer the sample carrier to the incubation component 2 after each addition of the reagent, and cover each reagent in an inverted manner for incubation in sequence until a completely incubated sample carrier is obtained after N incubations; wherein N is a positive integer.

[0046] It should be understood that all relevant contents of each step involved in the above method embodiment can be referred to the functional description of the corresponding functional module, which will not be repeated here. In addition, the use of suffixes such as "module", "component" or "unit" used to represent elements is only for the purpose of facilitating the description of the present invention, and has no specific meaning in itself. Therefore, "module", "component" or "unit" can be used in a mixed manner. The terminal can be implemented in various forms. For example, the terminal described in the present invention may include mobile terminals such as mobile phones, tablet computers, laptops, PDAs, portable media players (PMP), navigation devices, wearable devices, smart bracelets, pedometers, and fixed terminals such as digital TVs and desktop computers. The subsequent description will be described by taking the mobile terminal as an example, and those skilled in the art will understand that, in addition to components specifically used for mobile purposes, the construction according to the embodiment of the present invention can also be applied to fixed types of terminals.

[0047] In some other embodiments of the present invention, an electronic device 300 is disclosed. Figure 3 As shown, it may include: one or more processors 301; memory 302; display 303; one or more applications (not shown); and one or more computer programs 304. The above-mentioned components may be connected via one or more communication buses 305. The one or more computer programs 304 are stored in the above-mentioned memory 302 and configured to be executed by the one or more processors 301. The one or more computer programs 304 include instructions, which may be used to execute the following instructions: Figure 1 The various steps in the corresponding embodiments.

[0048] The processor 301 may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor or the processor may be any conventional processor, etc.

[0049] The memory 302 may be an internal storage unit of the electronic device 300, such as a hard disk or memory of the electronic device 300. The memory 302 may also be an external storage device of the electronic device 300, such as a plug-in hard disk, a smart media card (SMC), a secure digital (SD) card, a flash card (FlashCard), etc. equipped on the electronic device 300. Further, the memory 302 may also include both an internal storage unit of the electronic device 300 and an external storage device. The memory 302 is used to store computer programs and other programs and data required by the electronic device. The memory 302 may also be used to temporarily store data that has been output or is to be output.

[0050] The computer program 304 may be divided into one or more modules / units. The one or more modules / units may be a series of computer program instruction segments capable of performing specific functions. The instruction segments are used to describe the execution process of the computer program 304 in the electronic device 300 .

[0051] In addition to the above structure, those skilled in the art can understand that Figure 3 It is only an example of the electronic device 300 and does not constitute a limitation of the electronic device 300. The electronic device 300 may include more or fewer components than shown in the figure, or a combination of certain components, or different components. For example, the electronic device may also include input and output devices, network access devices, buses, etc.

[0052] Those skilled in the art can clearly understand that for the convenience and simplicity of description, only the division of the above-mentioned functional units and modules is used as an example for illustration. In practical applications, the above-mentioned function allocation can be completed by different functional units and modules as needed, that is, the internal structure of the device can be divided into different functional units or modules to complete all or part of the functions described above. The functional units and modules in the embodiment can be integrated into a processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The above-mentioned integrated unit can be implemented in the form of hardware or in the form of software functional units. In addition, the specific names of the functional units and modules are only for the convenience of distinguishing each other, and are not used to limit the scope of protection of the present invention. The specific working process of the units and modules in the above-mentioned system can refer to the corresponding process in the aforementioned method embodiment, which will not be repeated here.

[0053] Based on the above embodiments, the present invention further discloses a computer-readable storage medium having at least one computer program stored thereon, and when the computer program is executed by a processor, the control method of high-voltage immunohistochemistry in the above embodiments is implemented.

[0054] A person of ordinary skill in the art can understand that all or part of the steps in the method for implementing the above embodiment can be completed by instructing a processor through a program, and the program can be stored in a computer-readable storage medium, and the storage medium is a non-transitory medium, such as a random access memory, a read-only memory, a flash memory, a hard disk, a solid-state hard disk, a magnetic tape, a floppy disk, an optical disc, and any combination thereof. The above storage medium can be any available medium that can be accessed by a computer or a data storage device such as a server or a data center that includes one or more available media. The available medium can be a magnetic medium (e.g., a floppy disk, a hard disk, a tape), an optical medium (e.g., a digital video disc (DVD)), or a semiconductor medium (e.g., a solid-state disk (SSD)), etc.

[0055] The descriptions of the processes or structures corresponding to the above-mentioned figures have different emphases. For parts that are not described in detail in a certain process or structure, please refer to the relevant descriptions of other processes or structures.

[0056] In summary, the control method, device, equipment and medium of high-pressure immunohistochemistry disclosed in the present invention not only reduce the need for manual operation, but also greatly improve the processing speed and accuracy, effectively improve the automation level and efficiency of the experiment, and ensure the accuracy and reliability of the experimental results.

[0057] Although the embodiments of the present invention are described in detail above, it is obvious to those skilled in the art that various modifications and variations can be made to these embodiments. However, it should be understood that such modifications and variations are within the scope and spirit of the present invention as described in the claims. Moreover, the present invention described herein may have other embodiments and may be implemented or realized in a variety of ways.

Claims

1. A control method for high-pressure immunohistochemistry, characterized in that: include: Controlling the first robotic arm to sequentially add N reagents to the incubation component on the workbench; After each addition of reagent, the second robot arm is controlled to transfer the sample carrier to the incubation component by adsorption, and cover each reagent in an inverted manner for incubation in sequence, until a completely incubated sample carrier is obtained after N incubations; Wherein, N is a positive integer.

2. The method according to claim 1, characterized in that The N reagents are one or more combinations of blocking agents, primary antibody reagents, amplifiers, secondary antibody reagents, substrates and staining agents.

3. The method according to claim 1, characterized in that When the value of N is five, the first robot arm is controlled to sequentially add N reagents to the incubation component on the workbench as follows: The first robot arm is controlled to sequentially add a blocking agent, a primary antibody reagent, an amplifier or enhancer, a secondary antibody reagent and a substrate to the incubation component.

4. The method according to claim 3, characterized in that After each addition of reagent, the second robot arm is controlled to transfer the sample carrier to the incubation component by adsorption, and then covered on each reagent in an inverted manner for incubation in sequence until N incubations are completed to obtain a fully incubated sample carrier. Specifically: After adding the blocking agent, controlling the second robot arm to transfer the first sample carrier to the incubation component by adsorption, and covering the first sample carrier in an inverted manner on the blocking agent for the first incubation to obtain a second sample carrier; After adding the primary antibody reagent, controlling the second robot arm to adsorb and transfer the second sample carrier to the incubation component, and covering the first antibody reagent in an inverted manner for a second incubation to obtain a third sample carrier; After adding the amplifying agent, controlling the second mechanical arm to transfer the third sample carrier to the incubation component by adsorption, and covering the amplifying agent in an inverted manner for a third incubation to obtain a fourth sample carrier; After adding the secondary antibody reagent, the second robot arm is controlled to transfer the fourth sample carrier to the incubation component by adsorption, and the fourth sample carrier is covered on the secondary antibody reagent in an inverted manner for a fourth incubation to obtain a fifth sample carrier; After adding the substrate, the second robot arm is controlled to transfer the fifth sample carrier to the incubation component by adsorption, and covers the fifth sample carrier on the substrate in an inverted manner for incubation for the fifth time to obtain a completely incubated sample carrier.

5. The method according to claim 4, characterized in that The sample carrier is washed after the first incubation, after the second incubation, after the third incubation, and after the fourth incubation.

6. The method according to claim 1, characterized in that Before controlling the first robot arm to sequentially add N reagents to the incubation component on the workbench, the method further includes: A plurality of sample carriers carrying samples are dewaxed and thermally repaired in a thermal repair reaction device to obtain treated sample carriers.

7. The method according to claim 6, characterized in that Also includes: The third robot arm is controlled to place all sample carriers in the thermal repair reaction device on the transfer module for temporary storage, so as to transfer the sample carriers in the transfer module to the incubation component in batches.

8. The method according to any one of claims 1 to 7, characterized in that: Also includes: During the adsorption and transfer, the identification information on the sample carrier is scanned and the sample carrier information is recorded.

9. A high-voltage immunohistochemistry control device, used in the method according to any one of claims 1 to 7, characterized in that: include: A liquid adding control module, used for controlling the first robotic arm to sequentially add N kinds of reagents to the incubation component on the workbench; The transfer control module is used to control the second robot arm to transfer the sample carrier to the incubation component after each reagent is added, and cover each reagent in an inverted manner for incubation in sequence until a completely incubated sample carrier is obtained after N incubations; Wherein, N is a positive integer.

10. An electronic device, characterized in that: The electronic device comprises a memory and a processor, wherein the memory stores a program executable on the processor, and when the program is executed by the processor, the electronic device implements the method according to any one of claims 1 to 8.

11. A readable storage medium, wherein a program is stored in the readable storage medium, characterized in that: When the program is executed, the method according to any one of claims 1 to 8 is implemented.

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