Composition for coating plant seeds

Abstract

Provided is a composition for coating a plant seed, which is capable of promoting initial breeding of a plant or promoting breeding under stress conditions. A composition for coating a plant seed according to one or more embodiments of the present invention contains allantoin, an amino acid, and an agriculturally acceptable carrier. Preferably, the amino acid is L-tryptophan. Another embodiment of the present invention relates to a coated seed comprising a seed of a plant, and a composition comprising allantoin and an amino acid present on the surface of the seed.

Description

Technical Field

[0001] The present specification discloses a composition for coating plant seeds, coated seeds, a method for growing plants, a composition for promoting the initial growth of plants, and a composition for promoting the growth of plants under stress conditions. Background Art

[0002] As a method for applying a component useful for plant breeding to a plant, a method of coating seeds with a composition containing a useful component is known. Compared with a method of applying a useful component to a leaf surface, soil or water, this method can efficiently supply the useful component to a plant seed with a small amount of the useful component. Therefore, seed treatment based on a composition containing a useful component is expected to be used as a method for supplying useful components to plants with a small environmental burden and low cost.

[0003] Patent Document 1 describes a seed treatment composition comprising aromatic amino acids such as tryptophan, phenylalanine, and tyrosine and gibberellins. In addition, Patent Document 1 describes that wheat seed treatment based on tryptophan promotes germination of wheat seeds under normal conditions and high salt concentration conditions. In addition, Patent Document 1 describes that wheat seed treatment based on tryptophan and gibberellins can synergistically promote the increase in fresh weight of seedlings under normal conditions and high salt concentration conditions.

[0004] Patent Document 2 describes that the rooting promoting effect of plants can be greatly enhanced by combining phenyllactic acid with tryptophan, which has a rooting promoting effect on plants. Patent Document 2 also describes that seeds are treated with a phenyllactic acid-tryptophan mixture.

[0005] Allantoin (5-Ureidohydantoin) is an intermediate product produced during the decomposition of nucleic acid bases (purine bases). In plants, allantoin is produced by 5-hydroxyisouric acid through allantoin synthase (AS) and decomposed into allantoic acid by allantoinase (ALN). Non-patent document 1 discloses an aln-1 mutant, which has a mutation in the accumulation of allantoin in plants by destroying the ALN gene in Arabidopsis thaliana, and has high drought stress tolerance compared with wild-type strains.

[0006] Patent Document 3 discloses a high temperature stress tolerance enhancing agent containing allantoin as an active ingredient and used for improving the high temperature stress tolerance of plants.

[0007] Prior art literature

[0008] Patent Literature

[0009] Patent Document 1: International Publication No. 2018 / 215783

[0010] Patent Document 2: International Publication No. 2009 / 104405

[0011] Patent Document 3: International Publication No. 2017 / 130630

[0012] Non-patent literature: S. Watanabe, et al., Plant Cell Environ. (2014) 37, pp.1022-1036 Summary of the invention

[0013] Problems to be solved by the invention

[0014] In the cultivation of crop plants, it is important to promote the initial growth just after germination from seeds in order to increase the final yield. Therefore, a method capable of promoting the initial growth of plants is needed.

[0015] Additionally, there is a need for a method for improving plant growth under stress conditions such as salt stress.

[0016] Therefore, the present specification discloses a composition for coating plant seeds that can promote the initial growth of plants or promote growth under stress conditions.

[0017] In addition, the present specification discloses a method for growing coated seeds and plants using the above-mentioned composition.

[0018] In addition, the present specification discloses a composition for promoting the initial growth of a plant and a composition for promoting the growth of a plant under stress conditions.

[0019] Solutions to the problem

[0020] This specification discloses one or more of the following embodiments.

[0021] (1) A composition for coating plant seeds, comprising allantoin, an amino acid, and an agriculturally acceptable carrier.

[0022] (2) The composition according to (1), wherein

[0023] The amino acid is L-tryptophan.

[0024] (3) The composition according to (1) or (2), wherein

[0025] The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

[0026] (4) The composition according to any one of (1) to (3), wherein

[0027] The composition contains 10 g or more of allantoin in an amount for coating 1 ton of plant seeds.

[0028] (5) The composition according to any one of (1) to (4), wherein

[0029] The composition in an amount for coating 1 ton of plant seeds contains 0.045 g or more of the amino acid.

[0030] (6) The composition according to any one of (1) to (5), which is used for promoting the initial growth of a plant.

[0031] (7) The composition according to any one of (1) to (5), which is used for promoting plant growth under stress conditions.

[0032] (8) The composition according to any one of (1) to (5), which is used for increasing plant yield.

[0033] (9) A coated seed comprising:

[0034] Plant seeds, and

[0035] A composition present on the surface of the seeds and comprising allantoin and amino acids.

[0036] (10) The coated seed according to (9), wherein

[0037] The amino acid is L-tryptophan.

[0038] (11) The coated seed according to (9) or (10), wherein

[0039] The composition includes 0.009 parts by weight or more and 50 parts by weight or less of the amino acid relative to 100 parts by weight of the allantoin.

[0040] (12) The coated seed according to any one of (9) to (11), wherein

[0041] The coated seeds contain more than 10 g of allantoin per 1 ton.

[0042] (13) The coated seed according to any one of (9) to (12), wherein

[0043] The coated seeds contain more than 0.045 g of the amino acid per 1 ton.

[0044] (14) A method for cultivating a plant, the method comprising:

[0045] sowing the coated seeds according to any one of (9) to (13), and

[0046] The plants are grown from the coated seeds after sowing.

[0047] (15) The method according to (14), wherein:

[0048] The step of causing the plant to grow includes promoting initial growth of the plant.

[0049] (16) The method according to (14) or (15), wherein:

[0050] The step of growing the plants includes increasing the density of the plants, wherein the density is a value obtained by dividing the fresh weight of the above-ground parts of the plants by the plant height of the above-ground parts of the plants.

[0051] (17) The method according to any one of (14) to (16), wherein

[0052] The step of breeding the plant includes increasing the yield of the plant.

[0053] (18) The method according to any one of (14) to (17), wherein

[0054] The step of growing the plants comprises growing the plants under stress conditions.

[0055] (19) A composition for promoting the initial growth of a plant, comprising:

[0056] Allantoin, and

[0057] The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

[0058] (20) A composition for promoting plant growth under stress conditions, comprising:

[0059] Allantoin, and

[0060] The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

[0061] (21) A composition for increasing plant yield, comprising:

[0062] Allantoin, and

[0063] The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

[0064] This specification includes the disclosure of Japanese Patent Application No. 2022-152418 which is the basis of the priority of this application.

[0065] Effects of the Invention

[0066] The composition for coating plant seeds according to one or more embodiments of the present invention, the coated seeds using the composition, and the method for growing plants are effective for promoting the initial growth of plants and promoting growth under stress conditions.

[0067] The composition for promoting the initial growth of a plant according to one or more embodiments of the present invention can promote the initial growth of a plant by applying it to a plant.

[0068] The composition for promoting growth of plants under stress conditions according to one or more embodiments of the present invention can promote growth of plants under stress conditions by applying the composition to the plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0069] Figure 1 The graph shows the change rate (%) of the aboveground fresh weight of corn cultivated under normal conditions from seeds treated with different amounts of tryptophan and allantoin in Example 1 relative to the control plot.

[0070] Figure 2 The graph shows the change rate (%) of the aboveground fresh weight, density, and underground dry weight of corn cultivated under normal conditions from seeds treated with different amounts of tryptophan and allantoin in Example 1 relative to the control plot.

[0071] Figure 3 The graph shows the change rate (%) of the aboveground fresh weight of corn cultivated under salt stress conditions from seeds treated with different amounts of tryptophan and allantoin in Example 2 relative to the control plot.

[0072] Figure 4 The graph shows the change rate (%) of the aboveground fresh weight, density, and underground dry weight of corn cultivated under salt stress conditions from seeds treated with different amounts of tryptophan and allantoin in Example 2 relative to the control plot.

[0073] Figure 5 The graph shows the change rate (%) of the aboveground fresh weight of wheat cultivated under normal conditions from seeds treated with different amounts of tryptophan and allantoin in Example 3 relative to that of the control plot.

[0074] Figure 6 The graph shows the change rate (%) of the density of wheat cultivated under salt stress conditions from seeds treated with different amounts of tryptophan and allantoin in Example 4 relative to the control plot.

[0075] Figure 7The graph shows the change rate (%) of the above-ground fresh weight of soybeans grown under normal conditions from seeds treated with different amounts of tryptophan and allantoin in Example 5 relative to that of the control plot.

[0076] Figure 8 The graph shows the change rate (%) of the underground dry weight of soybeans cultivated under salt stress conditions from seeds treated with different amounts of tryptophan and allantoin in Example 6 relative to that of the control plot.

[0077] Fig. 9 The ear yield of corn cultivated in the field from corn seeds coated with 0.25 g tryptophan per 1 ton of seeds, corn seeds coated with 1000 g allantoin per 1 ton of seeds, and seeds coated with 0.25 g tryptophan and 1000 g allantoin per 1 ton of seeds in Example 7 is shown. DETAILED DESCRIPTION

[0078] Hereinafter, one or more embodiments of the present invention will be described.

[0079] <Allantoin>

[0080] Allantoin is also called 5-ureidohydantoin, and its free form has a structure represented by the following formula.

[0081]

[0082] Allantoin has one chiral carbon atom (represented by * in the formula), and there are optical isomers of (R)-allantoin and (S)-allantoin. The allantoin used in one or more embodiments of the present invention can be (R)-allantoin, (S)-allantoin, or a mixture thereof. Allantoin can be manufactured, for example, by synthesis from glyoxylic acid and urea. In addition, allantoin can also be derived from plants, microorganisms, or can be extracted from them.

[0083] Allantoin may include various forms such as a free form that is not bonded to other substances and is not ionized, a salt and a hydrate thereof, and a mixture of two or more thereof.

[0084] <Amino Acid>

[0085] In this specification, "amino acid" is any amino acid permitted in agriculture, preferably tryptophan. When the amino acid is an optically active amino acid, it may be an L-form, a D-form, or a mixture of an L-form and a D-form, preferably an L-form. The amino acid may be only one amino acid or a mixture of two or more amino acids. The most preferred amino acid is L-tryptophan.

[0086] The amino acid may include various forms of amino acids, such as free forms that are not bound to other substances and are not ionized, salts formed from amino acids and acids or bases, hydrates thereof, and mixtures thereof.

[0087] <Carriers permitted in agriculture>

[0088] In this specification, "agriculturally acceptable carrier" can be any carrier that can hold allantoin and amino acids, and can be a liquid carrier or a solid carrier. As a solid carrier, a hydratable solid can be mentioned. The solid carrier can be in the form of powder or granules.

[0089] Preferred "agriculturally acceptable carriers" are liquid carriers such as water and organic solvents. Here, the water used as a carrier is not limited to pure water, and may be an aqueous solution, an aqueous suspension, an aqueous gel or an aqueous slurry, and may have viscosity. Similarly, the organic solvent is not limited to a pure organic solvent, and may be a solution, a suspension, a gel or a slurry based on an organic solvent, and may have viscosity. Examples of the organic solvent include methyl ether, ethyl ether, propyl ether and butyl ether.

[0090] The carrier permitted in agriculture is preferably a liquid carrier comprising an aqueous solution formed by dissolving a hydratable substance in water, or a solid carrier comprising a hydratable substance that can be dissolved in water. As the hydratable substance, examples include: polyvinyl pyrrolidone, random and block copolymers of alkylene oxide, vinyl acetate / vinyl pyrrolidone copolymers, alkylated vinyl pyrrolidone copolymers, polyalkylene glycols comprising polypropylene glycol and polyethylene glycol, polyvinyl acetate, polyvinyl alcohol, gelatin, agar, gum arabic, karaya gum, tragacanth gum, guar gum, locust bean gum, xanthan gum, ghattigum, carrageenan, alginate, casein, dextran, pectin, chitin, 2-hydroxyethyl starch, 2-aminoethyl starch, 2-hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose salts, cellulose sulfate, polyacrylic acid amide, alkali metal salts of maleic anhydride copolymers, alkali metal salts of poly (meth) acrylates, etc.

[0091] Plant seed coating composition can further comprise one or more of the following further additives as required. As additives, wetting agent, colorant, defoamer, UV protectant, antifreeze, preservative, biological control agent or biocide, surfactant, emulsifier, extender, capture agent, plasticizer, phospholipid, flow agent, fusion aid, wax, and / or filler (for example, clay, talcum, glass fiber, cellulose, micronized wood, etc.) can be listed, but it is not limited to this.

[0092] <Plants>

[0093] In one or more embodiments of the present invention, "plant" is not particularly limited, and is preferably a crop plant. Examples of crop plants include corn (corn, maize), wheat, barley, rye, oats, rice, soybeans, rapeseed (Brassica napus), cotton, sunflowers, sugar beets, potatoes, tobacco, broccoli, lettuce, cabbage, cauliflower, coconut, tomato, cucumber, eggplant, honeydew melon, pumpkin, okra, pepper, watermelon, carrot, radish, onion, onion, flowers, lawn grass, forage grass, etc. In one or more embodiments of the present invention, "seed" can be seeds of these plants.

[0094] <Composition for coating plant seeds>

[0095] A first embodiment of the present invention relates to a composition for coating plant seeds, comprising allantoin, an amino acid, and an agriculturally acceptable carrier.

[0096] By treating the seeds of the plant with the composition for coating plant seeds of the present embodiment, the breeding of the seeds to plants, especially the initial breeding after germination, can be promoted. Here, "initial breeding" generally refers to the middle of the vegetative growth period from germination until the dry matter production begins to increase rapidly. For example, it refers to breeding within 90 days, within 60 days, within 30 days, within 25 days, within 21 days or within 14 days after germination.

[0097] In addition, by treating plant seeds with the composition for coating plant seeds according to the present embodiment, growth under stress conditions such as salt stress can be promoted.

[0098] The value obtained by dividing the aboveground fresh weight of the plant body by the plant height is defined as "density". The plant seed coating composition of the present embodiment is particularly capable of increasing the "aerial fresh weight", "density" and "underground dry weight" of the plant. According to the plant seed coating composition of the present embodiment, these effects can be used to increase the yield of the plant. Here, the increase in plant yield refers to the increase in the weight of the plant body parts harvested as crops, and is particularly preferably the increase in the weight of fruits, seeds, leaves, stems or roots harvested as crops.

[0099] That is, the composition for coating plant seeds of this embodiment is a composition for promoting the initial growth of plants, a composition for promoting the growth of plants under stress conditions, a composition for increasing the yield of plants, or a composition for increasing the density of plants (as defined above).

[0100] According to the different forms of the carrier allowed in the above-mentioned agriculture, the seed plant coating composition of the present embodiment can be liquid or solid accordingly. When the seed plant coating composition of the present embodiment is a liquid composition, the seed of the plant can be immersed or sprayed with the above-mentioned liquid composition and coated, and dried as required after coating. When the seed plant coating composition of the present embodiment is a solid composition such as powder, the above-mentioned solid composition after drying can be contacted with the seed of the plant and coated, and it can also be contacted with the seed of the plant and coated after adding water in the above-mentioned solid composition and making a liquid or pasty state, and dried as required after coating.

[0101] In the composition for coating seeds and plants of this embodiment, the amino acid is L-tryptophan. By coating plant seeds with a combination of allantoin and L-tryptophan, plant growth can be particularly significantly promoted.

[0102] The seed plant coating composition of the present embodiment comprises 0.009 weight part or more of amino acids relative to 100 weight parts of allantoin, preferably comprises 0.0225 weight part or more, more preferably comprises 0.045 weight part or more, and the upper limit comprises 50 weight parts or less, preferably comprises 20 weight parts or less, and particularly preferably comprises 18 weight parts or less relative to 100 weight parts of allantoin. When the weight ratio of allantoin to amino acids is within this range, the effect of synergistically promoting the breeding of plants is particularly significant.

[0103] The concentration of allantoin and amino acid in the seed plant coating composition of the present embodiment is not particularly limited. The above-mentioned seed plant coating composition of the amount used for coating 1 ton of plant seeds preferably contains more than 10g of allantoin, more preferably contains 25g, particularly preferably contains more than 50g, the upper limit is not particularly limited, preferably contains less than 1000g, more preferably contains less than 500g. In addition, the above-mentioned seed plant coating composition of the amount used for coating 1 ton of plant seeds preferably contains more than 0.045g of amino acid (especially L-tryptophan), particularly preferably contains more than 0.225g, the upper limit is not particularly limited, preferably contains less than 9g, particularly preferably contains less than 5g.

[0104] <Coated seeds>

[0105] A second embodiment of the present invention relates to a coated seed, comprising:

[0106] Plant seeds, and

[0107] A composition present on the surface of the above seeds and comprising allantoin and amino acids.

[0108] The coated seeds of the present embodiment can be prepared by coating plant seeds with the composition for coating plant seeds of the first embodiment of the present invention.

[0109] Plants grown from the coated seeds of this embodiment have high initial growth properties and high growth properties under stress conditions.

[0110] In the case of the coated seeds of the present embodiment, the composition containing allantoin and amino acids present on the surface of the seeds is typically the composition for coating plant seeds of the first embodiment of the present invention itself, or a composition obtained by drying the composition for coating plant seeds. It should be noted that, in the case of the coated seeds of the present embodiment, the composition containing allantoin and amino acids is not limited to being present only on the surface of the seeds, and a portion of the composition can penetrate into the inside of the seeds.

[0111] In the coated seeds of this embodiment, the amino acid is preferably L-tryptophan. By coating plant seeds with a combination of allantoin and L-tryptophan, plant growth can be particularly significantly promoted.

[0112] The above-mentioned composition in the coated seeds of the present embodiment particularly preferably contains 0.009 weight parts or more and 50 weight parts or less of amino acids relative to 100 weight parts of allantoin. The lower limit is not particularly limited as long as it is 0.009 weight parts or more, and is preferably 0.0225 weight parts or more, and more preferably 0.045 weight parts or more. In addition, the upper limit is also not particularly limited as long as it is 50 weight parts or less, but is preferably 20 weight parts or less, and more preferably 18 weight parts or less. When the weight ratio of allantoin to amino acids is within this range, the effect of synergistically promoting plant breeding is particularly significant.

[0113] The content of allantoin and amino acids in the coated seeds of the present embodiment is not particularly limited. On average, the coated seeds of the present embodiment preferably contain 10 g or more of allantoin per 1 ton, more preferably contain 25 g or more, and particularly preferably contain 50 g or more. The upper limit is not particularly limited, preferably 1000 g or less, and more preferably 500 g or less. In addition, the coated seeds of the present embodiment preferably contain 0.045 g or more of amino acids (especially L-tryptophan) per 1 ton, and particularly preferably contain 0.225 g or more. The upper limit is not particularly limited, preferably 9 g or less, and particularly preferably 5 g or less.

[0114] <How to grow plants>

[0115] A third embodiment of the present invention relates to a method for cultivating a plant, the method comprising:

[0116] sowing the coated seeds according to the second embodiment of the present invention, and

[0117] The plants are grown from the coated seeds after sowing.

[0118] According to the method of the third embodiment of the present invention, plant growth, especially early growth, can be promoted, and plants can be cultivated with high yield. In addition, plants can be cultivated efficiently even under stress conditions such as salt stress.

[0119] In the method of the present embodiment, the step of sowing the coated seeds and the step of growing the plant from the sown coated seeds can be performed under conditions appropriately selected according to the plant.

[0120] In the method of this embodiment, in the step of growing the plant from the sown coated seeds, growth, particularly early growth, is promoted. Here, the promotion of growth is preferably an increase in the above-ground fresh weight and / or density (defined as above) of the plant.

[0121] The degree of increase in the aboveground fresh weight of the method according to the present embodiment is not particularly limited, and the rate of increase in the aboveground fresh weight is preferably 1% or more, more preferably 2% or more, further preferably 5% or more, and particularly preferably 10% or more, compared to the case where the plant is cultivated from seeds that are not coated with the above-mentioned plant seed coating composition. From the viewpoint of market value, the upper limit of the rate of increase in the above-ground fresh weight is not particularly limited, but is preferably 100% or less, and particularly preferably 80% or less.

[0122] The degree of increase in density based on the method of this embodiment is not particularly limited, and the rate of increase in density is preferably 1% or more, more preferably 2% or more, further preferably 5% or more, and particularly preferably 10% or more, compared to the case where plants are cultivated from seeds that are not coated with the above-mentioned plant seed coating composition. From the viewpoint of market value, the upper limit of the rate of increase in the above-mentioned density is not particularly limited, but is preferably 100% or less, and particularly preferably 80% or less.

[0123] In the method of this embodiment, the step of growing the plant from the sown coated seeds includes, for example, growing the plant under stress conditions. Here, examples of stress conditions include salt stress conditions, high temperature stress conditions, and low temperature stress conditions.

[0124] <Other Compositions>

[0125] A fourth embodiment of the present invention relates to a composition for promoting initial growth of a plant, comprising: allantoin; and 0.009 parts by weight or more and 50 parts by weight or less of an amino acid per 100 parts by weight of the allantoin.

[0126] A fifth embodiment of the present invention relates to a composition for promoting plant growth under stress conditions, comprising: allantoin; and 0.009 parts by weight or more and 50 parts by weight or less of an amino acid per 100 parts by weight of the allantoin.

[0127] A sixth embodiment of the present invention relates to a composition for increasing plant yield, comprising: allantoin; and 0.009 parts by weight or more and 50 parts by weight or less of an amino acid per 100 parts by weight of the allantoin.

[0128] The compositions of the fourth, fifth and sixth embodiments of the present invention are not limited to seed coating compositions, and may be compositions for application to plants via appropriate routes, such as compositions for application to leaves or the like, compositions for application to soil or water.

[0129] The composition according to the fourth embodiment of the present invention can particularly significantly promote the initial growth of plants due to the synergistic effect of allantoin and amino acids.

[0130] The composition of the fifth embodiment of the present invention can significantly promote the initial growth of plants under stress conditions through the synergistic effect of allantoin and amino acids. Here, examples of stress conditions include salt stress conditions, high temperature stress conditions, and low temperature stress conditions.

[0131] The composition according to the sixth embodiment of the present invention can significantly increase the yield of plants due to the synergistic effect of allantoin and amino acids.

[0132] In the fourth, fifth and sixth aspects of the present invention, the amino acid is more preferably L-tryptophan.

[0133] Example

[0134] The present invention will be specifically described by way of the following examples, but the present invention is not limited to the examples.

[0135] In the following description and the drawings to be referred to, "tryptophan" or "Trp" means L-tryptophan, and "ALN" means allantoin.

[0136] <Example 1>

[0137] Corn cultivation from coated seeds under normal conditions

[0138] Experimental methods

[0139] Using a coating apparatus (manufactured by SATEC), 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of dent corn seeds (variety: Snow-dent Otoha). The coating treatment was performed individually or in combination with 0 g, 0.045 g, 0.225 g, or 0.45 g of tryptophan per ton (MT) of seeds and 0 g, 50 g, or 500 g of allantoin per ton of seeds.

[0140] The soil was filled into a 12 cm plastic pot, and 4 dent corn seeds were sown per pot after the coating treatment. After germination, one plant with different breeding levels was thinned out to 3 plants per pot. It was cultivated in a glass greenhouse and investigated on the 21st day after sowing. During the investigation, the fresh weight of the aboveground part and the dry weight of the underground part were measured, and the density (fresh weight of the aboveground part / plant height) was calculated. The dry weight of the underground part was obtained by measuring the weight after washing the underground part with water and drying it at 80°C for 2 days. The test was repeated 6 times and the average value was calculated.

[0141] result

[0142] The aboveground fresh weight, density, and underground dry weight measured for each test plot with different treatment amounts of tryptophan and allantoin are shown as the change rate (%) of the aboveground fresh weight, density, and underground dry weight of the test plot treated with a coating solution containing no tryptophan and allantoin (control plot). Figure 1 ; The results of aboveground fresh weight, density, and underground dry weight are shown in Figure 2 .

[0143] like Figure 1 As shown in the results, when only tryptophan was applied to seeds, the aboveground fresh weight after 3 weeks of sowing was -7% to 7% relative to no treatment, and a certain initial growth promotion effect was confirmed in the case of tryptophan alone. In addition, when only allantoin was applied to seeds, the aboveground fresh weight increased by 5%, and a certain initial growth promotion effect was also confirmed in the case of allantoin alone. In addition, by combining allantoin and tryptophan, the aboveground fresh weight increased by 11% to 19%, showing a surprising initial growth promotion effect through the synergistic effect of allantoin and tryptophan.

[0144] like Figure 2 As shown, in addition to the aboveground fresh weight, the density and underground dry weight also showed a surprising initial growth-promoting effect based on the synergistic effect of the combination of allantoin and tryptophan.

[0145] The increase in the fresh weight of the aboveground part can also be considered as leggy growth, that is, the plant mainly grows in elongation without the growth of full content. If leggy growth occurs, the plant will be sickly / weak and less resistant to pests than normal plants, and will be easily affected by environmental changes such as heat and cold, so it is not preferred. Therefore, in order to evaluate the quality of short and strong growth rather than leggy growth, density was used as an indicator for evaluation. Figure 2 As shown, in the case of the combination of allantoin and tryptophan, the density was synergistically increased compared with the case of each of the two plants, and an increase in yield due to the promotion of initial growth can be expected.

[0146] <Example 2>

[0147] Maize cultivation from coated seeds under salt stress conditions

[0148] Experimental methods

[0149] Using a coating apparatus (manufactured by SATEC), 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of dent corn seeds (variety: Snow-dent Otoha). The coating treatment was performed individually or in combination in a manner that tryptophan was 0 g, 0.045 g, 0.225 g, or 5 g per ton of seeds, and allantoin was 0 g, 10 g, 25 g, 50 g, or 500 g per ton of seeds.

[0150] The soil mixed with sodium chloride in the form of 6.0g / L soil was filled in a 7.5cm pot, and dent corn seeds after coating were sown 1 grain / pot. Cultivated in a glass greenhouse, and investigated on the 21st day after sowing. During the investigation, plant height, aboveground fresh weight, and aboveground dry weight were measured, and density (aerial fresh weight / plant height) was calculated. The test was repeated 9 times to obtain an average value.

[0151] result

[0152] Fresh weight of aboveground parts

[0153] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and allantoin are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with the coating solution containing no tryptophan and allantoin. Figure 3 .

[0154] like Figure 3As shown in the results, when only tryptophan was applied to seeds under salt stress, the aboveground fresh weight was -10% to -6% three weeks after sowing compared to no treatment, and no initial growth promotion effect was confirmed in the case of tryptophan alone. In addition, when only allantoin was applied to seeds, the difference in the amount of use was -14% to 27% compared to no treatment, showing a significant initial growth promotion effect. On the other hand, by combining allantoin and tryptophan, the aboveground fresh weight increased by -2% to 34%, showing a surprising initial growth promotion effect through the synergistic effect of allantoin and tryptophan.

[0155] Fresh weight of aboveground parts, density, dry weight of underground parts

[0156] The change rate (%) of the aboveground fresh weight, density (aboveground fresh weight / plant height) and underground dry weight measured or calculated for each test plot relative to the control plot was obtained. The aboveground fresh weight, density and underground dry weight of the control plot, tryptophan 0.225g / 1 ton of seeds, allantoin 50g / 1 ton of seeds, and tryptophan 0.225g + allantoin 50g / 1 ton of seeds are shown in Figure 4 .

[0157] like Figure 4 As shown, when the seeds were treated with a combination of 0.225 g of tryptophan and 50 g of allantoin per ton of seeds, the fresh weight of the aboveground part, density, and dry weight of the underground part were synergistically increased in the combination of allantoin and tryptophan compared to the single treatments, and a stable breeding-promoting effect brought about by the seed treatment could be confirmed under stress conditions.

[0158] <Example 3>

[0159] Wheat cultivation from coated seeds under normal conditions

[0160] Experimental methods

[0161] A coating treatment was performed using a coating device (manufactured by SATEC Co., Ltd.) by coating 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water on 100 g of wheat seeds (Yumechikara). The coating treatment was performed individually or in combination in a manner that tryptophan was 0 g, 0.045 g, 0.45 g, 4.5 g, or 9 g per ton of seeds, and allantoin was 0 g or 50 g.

[0162] The soil was filled to 12 cm plastic pots, and 4 wheat seeds after coating were sown in each pot. After germination, 1 plant with different breeding levels was thinned out to 3 plants per pot. The plants were cultivated in a glass greenhouse and investigated 2 weeks after sowing. The test was repeated 6 times and the average value was calculated.

[0163] result

[0164] Fresh weight of aboveground parts

[0165] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and allantoin are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and allantoin. Figure 5 .

[0166] like Figure 5 As shown in the results, when only tryptophan was applied to seeds, the aboveground fresh weight after 2 weeks of sowing was -2% to -1% compared to no treatment, and no initial growth promoting effect was confirmed in the case of tryptophan alone. In addition, when only allantoin was applied to seeds, it was 1%, and no initial growth promoting effect was confirmed in allantoin alone. On the other hand, by combining allantoin and tryptophan, the aboveground fresh weight increased by 4% to 8%, showing a surprising initial growth promoting effect through the synergistic effect of allantoin and tryptophan.

[0167] <Example 4>

[0168] Wheat cultivation from coated seeds under salt stress conditions

[0169] Experimental methods

[0170] A coating treatment was performed using a coating device (manufactured by SATEC) by coating 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water on 100 g of wheat seeds (variety: Quintus). The coating treatment was performed individually or in combination in a manner that tryptophan was 0 g, 0.045 g, 0.45 g, 4.5 g, or 9 g per ton of seeds, and allantoin was 0 g or 50 g.

[0171] The soil mixed with sodium chloride in the form of 4.0g / L soil was filled in a 7.5cm pot, and wheat seeds after coating were sown 1 grain / pot. Cultivated in a glass greenhouse, and investigated on the 16th day after sowing. During the investigation, plant height and aboveground fresh weight were measured, and density (aerial fresh weight / plant height) was calculated. The test was repeated 9 times to obtain an average value.

[0172] result

[0173] density

[0174] The density measured in each test area with different treatment amounts of tryptophan and allantoin is shown as a change rate (%) relative to the density of the test area (control area) treated with a coating solution containing no tryptophan and allantoin. Figure 6 .

[0175] like Figure 6As shown, under salt stress conditions, when only tryptophan was applied to seeds, the density after 2 weeks of sowing was 1% to 8% relative to no treatment, and in the case of tryptophan alone, a certain initial breeding promotion effect was shown. In addition, when only allantoin was applied to seeds, it was 3% relative to no treatment, which also showed a certain initial breeding promotion effect. In addition, by combining allantoin with tryptophan, the density was increased by 7% to 18%, and a surprising initial breeding promotion effect was shown by the synergistic effect of allantoin and tryptophan.

[0176] <Example 5>

[0177] Soybean cultivation from coated seeds under normal conditions

[0178] Experimental methods

[0179] 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of soybean seeds (variety: Fukuyutaka) using a coating apparatus (manufactured by SATEC). The coating treatment was performed individually or in combination in such a manner that 0 g, 0.225 g, 0.45 g, or 5 g of tryptophan and 0 g or 25 g of allantoin were applied per ton of seeds.

[0180] The soil was filled to 12 cm plastic pots, and 4 soybean seeds after coating were sown in each pot. After germination, 1 plant with different breeding levels was thinned out to 3 plants per pot. The plants were cultivated in a glass greenhouse and investigated 3 weeks after sowing. The test was repeated 6 times and the average value was calculated.

[0181] result

[0182] Fresh weight of aboveground parts

[0183] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and allantoin are shown as the change rate (%) of the aboveground fresh weight of the control plot treated with a coating solution containing no tryptophan and allantoin. Figure 7 .

[0184] like Figure 7 As shown, when only tryptophan was applied to seeds, the aboveground fresh weight after 3 weeks of sowing was -1% to 4% relative to no treatment. In the case of tryptophan alone, a certain initial breeding promotion effect was shown depending on the amount used. In addition, when only allantoin was applied to seeds, it was 4%, and allantoin alone also showed a certain initial breeding promotion effect. On the other hand, by combining allantoin and tryptophan, the aboveground fresh weight increased by 10% to 19%, and a surprising initial breeding promotion effect was shown through the synergistic effect of allantoin and tryptophan.

[0185] <Example 6>

[0186] Soybean cultivation from coated seeds under salt stress conditions

[0187] Experimental methods

[0188] 800 μL of a coating solution containing tryptophan, allantoin, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of soybean seeds (variety: Fukuyutaka) using a coating apparatus (manufactured by SATEC). The coating treatment was performed individually or in combination in such a manner that 0 g, 0.045 g, 0.225 g, 0.45 g, or 5 g of tryptophan and 0 g or 50 g of allantoin were present per ton of seeds.

[0189] The soil mixed with sodium chloride in the form of 1.0g / L soil was filled into a 7.5cm pot, and 1 soybean seed after coating was sown per pot. The soil was cultivated in a glass greenhouse and investigated 3 weeks after sowing. During the investigation, the underground dry weight was measured. The test was repeated 9 times and the average value was calculated.

[0190] result

[0191] Dry weight of underground part

[0192] The underground dry weight measured for each test plot with different treatment amounts of tryptophan and allantoin is shown as a change rate (%) relative to the underground dry weight of the control plot treated with a coating solution containing no tryptophan and allantoin. Figure 8 .

[0193] like Figure 8 As shown, under salt stress conditions, when only tryptophan was applied to seeds, the underground dry weight after sowing for 3 weeks was -6% to 8% relative to no treatment, and in the case of tryptophan alone, a certain initial growth promotion effect was shown according to the different usage amounts. In addition, when only allantoin was applied to seeds, it was 2% relative to no treatment, showing a certain initial growth promotion effect. On the other hand, by combining allantoin and tryptophan, the underground dry weight was increased by 12% to 17%, showing an unexpected initial growth promotion effect through the synergistic effect of allantoin and tryptophan.

[0194] <Example 7>

[0195] Corn field trials

[0196] Preparation of coated seeds

[0197] Corn seeds coated with 0.25 g of tryptophan per ton of seeds, corn seeds coated with 1000 g of allantoin per ton of seeds, and corn seeds coated with 0.25 g of tryptophan and 1000 g of allantoin per ton of seeds were prepared by the same method as in Example 1. Seeds not subjected to coating treatment were prepared as a control.

[0198] Field cultivation

[0199] Location: Fields of Wilderen MAR, Kingdom of Belgium

[0200] Type: Like IT

[0201] Planting date: April 27, 2020

[0202] Payoff date: October 12, 2020

[0203] Cultivation was carried out through routine fertilization management and pest and disease management, and a yield survey was implemented.

[0204] Yield survey: 120 plants / area, repeated 4 times

[0205] result

[0206] The ratios (%) of the ear yields of corn grown from the three types of coated seeds when the ear yield of corn grown from uncoated seeds in the control plot was set as 100% are shown in FIG. Fig. 9 .

[0207] like Fig. 9 As shown in the present invention, the coated seeds treated with 0.25g of tryptophan per 1 ton of seeds, 1000g of allantoin per 1 ton of seeds, and the combination of 0.25g of tryptophan and 1000g of allantoin per 1 ton of seeds were sown in the field and cultivated until harvest, and an investigation was conducted. Compared with the untreated area, the female ear yield in the case of tryptophan alone was 100.4%, and the female ear yield in the case of allantoin alone was 100.4%, and no increase in yield was observed. On the other hand, in the case of the combination of tryptophan and allantoin, the female ear yield in the untreated area was 102.5%, showing a synergistic effect of an increase of 2.5%, and the increase effect of the final yield brought about by the seed treatment can be confirmed.

[0208] All publications, patents and patent applications cited in this specification are incorporated herein by direct reference.

Claims

1. A composition for coating plant seeds, comprising allantoin, amino acids, and an agriculturally acceptable carrier.

2. The composition according to claim 1, wherein The amino acid is L-tryptophan.

3. The composition according to claim 1 or 2, wherein The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

4. The composition according to claim 1 or 2, wherein The composition contains 10 g or more of allantoin in an amount for coating 1 ton of plant seeds. The composition according to claim 1 or 2, which is used for promoting the initial growth of plants.

6. The composition according to claim 1 or 2, for use in promoting the growth of plants under stress conditions.

7. The composition according to claim 1 or 2, for use in increasing the yield of plants.

8. A coated seed comprising: Plant seeds, and A composition present on the surface of the seeds and comprising allantoin and amino acids.

9. The coated seed according to claim 8, wherein The amino acid is L-tryptophan.

10. The coated seed according to claim 8 or 9, wherein The composition includes 0.009 parts by weight or more and 50 parts by weight or less of the amino acid relative to 100 parts by weight of the allantoin.

11. The coated seed according to claim 8, wherein The coated seeds contain more than 10 g of allantoin per 1 ton.

12. A method for cultivating plants, the method comprising: sowing the coated seeds according to claim 8, and The plants are grown from the coated seeds after sowing.

13. The method according to claim 12, wherein: The step of causing the plant to grow includes promoting initial growth of the plant.

14. The method according to claim 12 or 13, wherein: The step of growing the plants comprises growing the plants under stress conditions.

15. A composition for promoting the initial propagation of a plant, comprising: Allantoin, and The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

16. A composition for promoting plant growth under stress conditions, comprising: Allantoin, and The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

17. A composition for increasing plant yield, comprising: Allantoin, and The amino acid is contained in an amount of 0.009 parts by weight or more and 50 parts by weight or less based on 100 parts by weight of the allantoin.

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