Compound synergistic bactericidal composition containing quinolone compounds and application thereof

Abstract

The invention relates to a synergistic bactericidal composition containing quinolone compounds and application of the synergistic bactericidal composition. The bactericide composition comprises effective active components, the effective active components comprise a component I and a component II, the component I is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, the component II is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, and the component III is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester. And the component II is selected from at least one of pydiflumetofen, pleuromutilin, chloroacetyl pleuromutilin and fatty alcohol polyoxyethylene ether carboxylic acid zinc. The synergistic bactericidal composition provided by the invention has the following advantages: 1) the synergistic bactericidal composition has a synergistic effect, can improve the prevention and treatment effect, and has a long lasting period; 2) the bactericidal spectrum is expanded, field diseases occur in a mixed manner, and the effect on bacterial diseases is stronger; 3) the action mechanisms of the two effective components are different, and the drug resistance of pathogenic bacteria can be delayed by mixing the two effective components; and 4) the application amount is reduced, the use frequency is reduced, and the use cost is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compounding, and in particular relates to a synergistic fungicide composition containing quinolone compounds and application thereof. Background Art

[0002] In recent years, the occurrence of bacterial diseases of various crops has shown a trend of increasing year by year, and the affected area has increased year by year, bringing great harm to agricultural production. For example, bacterial leaf streak of rice, white leaf blight of rice, bacterial brown streak of rice in grain crops, citrus canker, bacterial perforation of peach, kiwi canker in fruit trees, bacterial wilt of Solanaceae vegetables, bacterial angular leaf spot of cucumber, soft rot of cruciferous vegetables, ginger blight, etc., have all caused serious losses to crops.

[0003] The chemical name of quinolone compounds is: 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, which has a broad-spectrum fungicidal activity, especially against bacteria, and shows excellent activity against both Gram-positive and Gram-negative bacteria. It is effective against a variety of crop bacterial diseases such as Chinese cabbage soft rot and other crop soft rots, Chinese cabbage black rot, cucumber bacterial angular spot, sesame angular spot, melon / melon fruit spot, rice bacterial leaf blight, rice bacterial streak, rice bacterial brown spot, rice bacterial brown streak, rice bacterial base rot, tomato bacterial wilt and other Solanaceae bacterial wilt diseases, mulberry bacterial wilt, peanut bacterial wilt, ginger blight, tomato / pepper bacterial spot, pepper bacterial leaf spot, potato black shank, corn bacterial wilt, corn bacterial wilt, It has excellent activity against pathogenic bacteria such as fungal stem rot, wheat black glumes, soybean bacterial spot disease, soybean bacterial blight, cassava bacterial wilt, mango angular spot disease, citrus canker, peach bacterial punchhole disease, sunflower stem rot, peach gummosis, pear fire blight, pear rust water disease, fruit tree bacterial root cancer, potato ring rot, bean wilt, wheat white leaf streak, potato scab, tomato canker, American holly leaf blight, tobacco wildfire disease, pitaya bacterial scab, and Panax notoginseng bacterial root rot.

[0004] However, in the actual process of agricultural production, long-term continuous single use of the same agent will soon cause pathogens to develop resistance, resulting in reduced prevention effect, increased pesticide usage, and aggravated pesticide residues in agricultural products and damage to the ecological environment. At present, there is a lack of efficient agents for preventing and treating bacterial diseases on the market. In order to improve the prevention and control effect, farmers increase the dosage or mix the agents at will. This unscientific use of drugs not only fails to achieve synergistic effects, but also leads to waste of pesticides, excessive residues, environmental pollution, and resistance of pathogens to pesticides. Therefore, it is urgent to develop a synergistic bactericidal composition for bacterial diseases, which can achieve the effects of expanding the scope of prevention and control objects, reducing the dosage, and delaying the generation of resistance. Summary of the invention

[0005] In order to improve the deficiencies of the prior art, the object of the present invention is to provide a synergistic fungicidal composition containing quinolone compounds and its application, the fungicidal composition comprises an active ingredient, the active ingredient comprises component I and component II, wherein the component I is selected from 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid ethyl ester, and the compound component II is selected from at least one of fluopicolide, pleuromutilin, chloroacetyl pleuromutilin, and fatty alcohol polyoxyethylene ether zinc carboxylate.

[0006] The mixed use of the component I and the component II can form a synergistic fungicide composition. The effective active ingredients include component I and component II. The mixed use of the two has obvious synergistic and complementary effects. By utilizing the different action sites and action mechanisms of pathogens, the compound can act on multiple sites of pathogens, expand the fungicide spectrum, improve the prevention effect, reduce the amount of pesticide used, delay the development of drug resistance, and reduce costs.

[0007] The invention provides a bactericidal composition. The bactericidal composition comprises effective active ingredients, wherein the effective active ingredients comprise component I and component II, wherein the component I is selected from 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid ethyl ester, and the component II is selected from any one, two or more of fluopicolide, pleuromutilin, chloroacetyl pleuromutilin and fatty alcohol polyoxyethylene ether zinc carboxylate.

[0008] According to an embodiment of the present invention, the structural formula of component I is The structural formula of fluopicolide is The structural formula of pleuromutilin is The structural formula of chloroacetyl pleuromutilin is

[0009] According to an embodiment of the present invention, the structural formula of fatty alcohol polyoxyethylene ether zinc carboxylate is:

[0010] Zn[R(CH2CH2O)n CH2COO]2,

[0011] Wherein, R is selected from C6-C14 fatty alcohols and isomeric alcohols thereof (e.g., C10 fatty alcohols, C11 fatty alcohols, C12 fatty alcohols, C13 fatty alcohols, C14 fatty alcohols);

[0012] n is a natural number selected from 3-9 (e.g., 3, 4, 5, 6, 7, 8, 9).

[0013] According to an embodiment of the present invention, the fatty alcohol polyoxyethylene ether zinc carboxylate is selected from any one, two or more of the following:

[0014] Zn[C 14 H 29 O(CH2CH2O)9CH2COO]2, Zn[C 12 H 25 O(CH2CH2O)9CH2COO]2, Zn[C 13 H 27 O(CH2CH2O)9CH2COO]2 、 Zn[C 14 H 29 O(CH2CH2O)7CH2COO]2 、Zn[C 12 H 25 O(CH2CH2O)7CH2COO]2 、 Zn[C 13 H 27 O(CH2CH2O)7CH2COO]2 、Zn[C 14 H 29 O(CH2CH2O)5CH2COO]2 、 Zn[C 12 H 25 O(CH2CH2O)5CH2COO]2 、Zn[C 13 H 27 O(CH2CH2O)5CH2COO]2.

[0015] According to an embodiment of the present invention, the fungicide composition comprises component I and fluopicolide. According to an embodiment of the present invention, the fungicide composition comprises component I and pleuromutilin. According to an embodiment of the present invention, the fungicide composition comprises component I and chloroacetyl pleuromutilin. According to an embodiment of the present invention, the fungicide composition comprises component I and zinc fatty alcohol polyoxyethylene ether carboxylate.

[0016] According to an embodiment of the present invention, the mass ratio of component I to component II is 80-1:1-80. According to an embodiment of the present invention, the mass ratio of component I to component II is 50-1:1-50. According to an embodiment of the present invention, the mass ratio of component I to component II is 30-1:1-30. According to an embodiment of the present invention, the mass ratio of component I to component II is 80:1, 75:1, 70:1, 65:1, 60:1, 55:1, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 15:1, 10:1, 8:1, 5:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:5, 1:8, 1:10, 15:1, 1:20, 25:1, 1:30, 35:1, 1:40, 45:1, 1:50, 55:1, 1:60, 65:1, 1:70, 75:1, 1:80.

[0017] According to an embodiment of the present invention, based on the total weight of the bactericidal composition as 100%, the sum of the mass of component I and component II in the bactericidal composition is 0.1% to 90%, for example, 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 70%, 75%, 80%, 85%, 90%.

[0018] According to an embodiment of the present invention, based on the total weight of the bactericidal composition as 100%, the mass of component I in the bactericidal composition is 0.1% to 80%, for example, 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 70%, 75%, 80%.

[0019] According to an embodiment of the present invention, based on the total weight of the bactericidal composition as 100%, the mass of component II in the bactericidal composition is 0.1% to 80%, for example, 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 70%, 75%, 80%. According to an embodiment of the present invention, the bactericidal composition can be prepared into a liquid preparation or a solid preparation.

[0020] According to the embodiments of the present invention, the content range of the effective active ingredient in the preparation varies according to different preparation types.

[0021] According to an embodiment of the present invention, the liquid preparation contains 0.1% to 60% of effective active ingredients by weight, preferably 0.5% to 50%; for example, 0.1%, 0.2%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%.

[0022] According to an embodiment of the present invention, the solid preparation contains 0.5% to 80% of effective active ingredients by weight, preferably 1% to 70%; for example, 0.5%, 0.8%, 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 70%, 75%, 80%.

[0023] According to an embodiment of the present invention, the bactericidal composition optionally further comprises any one, two or more of an emulsifier, a dispersant, a wetting agent, a thickener, a defoaming agent, a stabilizer, a binder, a disintegrant, an antifreeze agent, an anticaking agent, a suspending agent, a film-forming agent, a preservative, a colorant, a polymer capsule wall material, a pH adjuster or a filler.

[0024] According to an embodiment of the present invention, the bactericidal composition can be diluted before use or used directly. Its preparation can be prepared by a processing method known to those skilled in the art, that is, the effective active ingredient is mixed with any one, two or more of deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze agent, anticaking agent, suspending agent, film former, preservative, colorant, polymer capsule wall material, pH regulator or filler, etc. to prepare the bactericidal composition.

[0025] According to the preparation provided by the present invention, the fungicidal composition can be made into various dosage forms, including liquid preparations or solid preparations. Preferably, the dosage forms include water-dispersible granules, dispersible liquids, wettable powders, suspensions, aqueous emulsions, microemulsions, suspoemulsions, microcapsule suspensions, microcapsule suspension-suspension concentrates, suspension seed coatings, emulsifiable concentrates, and granules.

[0026] According to an embodiment of the present invention, when the bactericidal composition is made into a wettable powder, it comprises the following components and contents: component I 1% to 80%, component II 1% to 80%, dispersant 1% to 20%, wetting agent 1% to 8%, and the balance is filler; preferably, it comprises the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 5%, and the balance is filler; illustratively, it comprises the following components and contents: component I 5% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), component II 5% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), dispersant 9% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%), wetting agent 2% (or 1%, 2%, 3%, 5%, 8%, 10%), the balance is filler. According to an embodiment of the present invention, the dispersant is selected from sodium lignin sulfonate. According to an embodiment of the present invention, the wetting agent is selected from sodium dodecylbenzene sulfonate. According to an embodiment of the present invention, the filler is selected from any one, two or more of talc, white carbon, attapulgite, and kaolin; preferably, the content of talc is 1% to 10%, such as 1%, 2%, 3%, 5%, 8%, 10%; preferably, the content of white carbon is 1% to 10%, such as 1%, 2%, 3%, 4%, 5%, 8%, 10%; preferably, the content of attapulgite is 50% to 80%, such as 50%, 55%, 60%, 65%, 68%, 70%, 72%, 75%, 80%. According to an embodiment of the present invention, when the bactericidal composition is made into a wettable powder, it comprises the following components and contents: component I 5%, component II 5%, dispersant 9%, wetting agent 2%, talc 3%, white carbon 4%, and the balance is attapulgite.

[0027] According to an embodiment of the present invention, when the composition is made into a water-dispersible granule, it includes the following components and contents: component I 1% to 80%, component II 1% to 80%, dispersant 1% to 20%, wetting agent 1% to 8%, disintegrant 1% to 10%, and the balance is filler; preferably, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 5%, disintegrant 1% to 8%, and the balance is filler; illustratively, it includes the following components and contents: component I 20% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), component II 10% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), dispersant 9% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%), wetting agent 4% (or 1%, 2%, 3%, 5%, 8%, 10%), disintegrant 4% (or 1%, 2%, 3%, 5%, 8%, 10%), the balance is filler. According to an embodiment of the present invention, the dispersant is selected from sodium lignin sulfonate. According to an embodiment of the present invention, the wetting agent is selected from sodium alkyl naphthalene sulfonate and sodium dodecyl sulfate. According to an embodiment of the present invention, the disintegrant is selected from ammonium sulfate. According to an embodiment of the present invention, the filler is selected from any one, two or more of talc, white carbon, attapulgite and kaolin. According to an embodiment of the present invention, when the composition is made into water dispersible granules, it includes the following components and contents: component I 20%, component II 10%, sodium alkylnaphthalene sulfonate 2%, sodium lignin sulfonate 9%, sodium dodecyl sulfate 2%, ammonium sulfate 4%, and the balance is kaolin.

[0028] According to an embodiment of the present invention, when the composition is made into a suspension, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 20%, wetting agent 1% to 10%, thickener 0.1% to 10%, antifreeze 0.1% to 8%, and the balance is water (e.g., deionized water); preferably, it includes the following components and contents: component I 1% to 30%, component II 1% to 30%, dispersant 1% to 12%, wetting agent 1% to 8%, thickener 0.1% to 8%, antifreeze 0.1% to 8%, and the balance is water; illustratively, it includes the following components and contents: component I 10% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), component II 10% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), wetting agent 5% (or 1%, 2%, 3%, 5%, 8%, 10%), thickener 7% (or 1%, 2%, 3%, 5%, 8%, 10%), dispersant 3% (or 1%, 2%, 3%, 5%, 8%, 10%), antifreeze agent 4% (or 1%, 2%, 3%, 5%, 8%, 10%), the balance is water. According to an embodiment of the present invention, the wetting agent is selected from methyl naphthalene sulfonate sodium formaldehyde condensate. According to an embodiment of the present invention, the thickener is selected from xanthan gum. According to an embodiment of the present invention, the dispersant is selected from naphthalenesulfonic acid formaldehyde condensate sodium salt. According to an embodiment of the present invention, the antifreeze agent is selected from propylene glycol. According to an embodiment of the present invention, when the composition is made into a suspension, it includes the following components and contents: component I 10%, component II 10%, sodium methyl naphthalene sulfonate formaldehyde condensate 5%, xanthan gum 7%, naphthalenesulfonic acid formaldehyde condensate sodium salt 3%, propylene glycol 4%, and the balance is water.

[0029] According to an embodiment of the present invention, when the composition is made into an aqueous emulsion, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, organic solvent 1% to 70%, emulsifier 1% to 20%, antifreeze 0.1% to 8%, defoamer 0.01% to 2%, thickener 0.1% to 2%, and the balance is water (e.g., deionized water); preferably, it includes the following components and contents: component I 1% to 30%, component II 1% to 30%, organic solvent 10% to 60%, emulsifier 1% to 12%, antifreeze 0.1% to 5%, defoamer 0.01% to 1%, thickener 0.1% to 1%, and the balance is water; illustratively, it includes the following components and contents: component I 10% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), component II 5% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), organic solvent 60% (or 5%, 8%, 10%, 13%, 15%, 18%, 20%, 24%, 25%, 30%, 35%, 38%, 40%, 42%, 45%, 50%, 55%, 60%, 65%, 68%, 70%), emulsifier 1 0% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%), antifreeze 3% (or 0.5%, 1%, 2%, 3%, 5%, 8%), defoamer 0.5% (or 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%), thickener 0.3% (or 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%), the balance is water. According to an embodiment of the present invention, the organic solvent is selected from: 2,2,2-trifluoroethanol, propylene carbonate, 150# solvent oil. According to an embodiment of the present invention, the emulsifier is selected from Span-80. According to an embodiment of the present invention, the antifreeze is selected from ethylene glycol. According to an embodiment of the present invention, the defoamer is selected from silicone oil. According to an embodiment of the present invention, the thickener is selected from polyvinyl alcohol. According to an embodiment of the present invention, when the composition is made into an aqueous emulsion, it includes the following components and contents: component I 10%, component II 5%, 2,2,2-trifluoroethanol 35%, propylene carbonate 10%, 150# solvent oil 15%, Span-80 10%, ethylene glycol 3%, silicone oil 0.5%, polyvinyl alcohol 0.3%, and the balance is water.According to an embodiment of the present invention, when the composition is made into a suspended seed coating agent, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 10%, antifreeze agent 1 to 10%, anticaking agent 0.1 to 10%, suspending agent 0.1-5%, film-forming agent 1 to 10%, preservative 0.1 to 5%, colorant 1 to 30%, pH adjuster 0.1% to 5%, thickener 0.1% to 8%, and the balance is water (e.g., deionized water).

[0030] According to an embodiment of the present invention, when the composition is made into a microcapsule suspension, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, polymer capsule wall material 1-30%, dispersant 2% to 10%, organic solvent 1 to 50%, emulsifier 1% to 7%, pH regulator 0.1% to 5%, defoamer 0.01% to 2%, thickener 0.1% to 8%, antifreeze 0.1% to 8%, and the balance is water (e.g., deionized water).

[0031] According to an embodiment of the present invention, when the composition is made into a microcapsule suspension-suspension concentrate, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, polymer capsule wall material 1% to 12%, dispersant 1% to 12%, wetting agent 1% to 8%, organic solvent 1-50%, emulsifier 1% to 8%, defoamer 0.01% to 2%, thickener 0.1% to 8%, pH regulator 0.1% to 5%, antifreeze 0.1% to 8%, and the balance is water (e.g., deionized water).

[0032] According to an embodiment of the present invention, when the composition is made into an emulsifiable concentrate, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is an organic solvent; preferably, it includes the following components and contents: component I 1% to 40%, component II 1% to 30%, emulsifier 1 to 20%, antifreeze agent 1% to 8%, stabilizer 0.1% to 3%, and the balance is an organic solvent; illustratively, it includes the following components and contents: component I 30% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), component II 20% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%, 18%, 20%, 25%, 30%), emulsifier 6% (or 1%, 2%, 3%, 5%, 8%, 10%, 13%, 15%), antifreeze 4% (or 0.5%, 1%, 2%, 3%, 5%, 8%), stabilizer 0.1% (or 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%), the balance is organic solvent. According to an embodiment of the present invention, the emulsifier is selected from tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, fatty alcohol polyethylene ether. According to an embodiment of the present invention, the antifreeze is selected from propylene glycol. According to an embodiment of the present invention, the stabilizer is selected from triethanolamine. According to an embodiment of the present invention, the organic solvent is selected from solvent oil. According to an embodiment of the present invention, when the composition is made into an emulsifiable concentrate, it includes the following components and contents: component I 30%, component II 20%, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt 4%, fatty alcohol polyvinyl ether 2%, propylene glycol 4%, triethanolamine 0.1%, and the balance is solvent oil. According to an embodiment of the present invention, when the composition is made into an emulsifiable concentrate, it includes the following components and contents: component I 10%, component II 20%, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt 4%, fatty alcohol polyvinyl ether 2%, propylene glycol 4%, triethanolamine 0.1%, and the balance is solvent oil. According to an embodiment of the present invention, when the composition is made into an emulsifiable concentrate, it includes the following components and contents: component I 10%, component II 15%, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt 4%, fatty alcohol polyvinyl ether 2%, propylene glycol 4%, triethanolamine 0.1%, and the balance is solvent oil.

[0033] According to an embodiment of the present invention, when the composition is made into a microemulsion, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, organic solvent 1 to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is water (e.g., deionized water).

[0034] According to an embodiment of the present invention, when the composition is made into a dispersible liquid, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is organic solvent.

[0035] According to an embodiment of the present invention, when the composition is made into granules, it includes the following components and contents: component I 0.5% to 10%, component II 0.5% to 10%, dispersant 1% to 12%, wetting agent 1% to 10%, binder 0.1% to 8%, and the balance is filler; preferably, it includes the following components and contents: component I 0.5% to 5%, component II 0.5% to 5%, dispersant 1% to 10%, wetting agent 1% to 8%, binder 0.1% to 5%, and the balance is filler; illustratively, it includes the following components and contents: component I 0.5% (or 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%), component II 0.5% (or 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 2%, 3%, 5%), dispersant 3.2% (or 0.5%, 1%, 2%, 3%, 5%, 8%, 10%), wetting agent 3.5% (or 0.5%, 1%, 2%, 3%, 5%, 8%, 10%), binder 2% (or 0.5%, 1%, 2%, 3%, 5%, 8%, 10%), the balance is filler. According to an embodiment of the present invention, the dispersant is selected from sodium lignin sulfonate. According to an embodiment of the present invention, the wetting agent is selected from sodium lauryl sulfate. According to an embodiment of the present invention, the binder is selected from polyvinyl alcohol. According to an embodiment of the present invention, when the composition is made into granules, it includes the following components and contents: component I 0.5%, component II 0.5%, sodium lignin sulfonate 3.2%, sodium lauryl sulfate 3.5%, polyvinyl alcohol 2%, and the balance is filler.

[0036] According to an embodiment of the present invention, the emulsifier is selected from any one, two or more of the following: sodium lignin sulfonate, agricultural milk, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, anhydrous sorbitan fatty acid ester polyoxyethylene ether, sorbitan anhydride oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctyl succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate.

[0037] According to an embodiment of the present invention, the dispersant is selected from any one, two or more of the following: glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignin sulfonate, naphthalene sulfonate formaldehyde condensation product, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensation product, naphthalenesulfonic acid formaldehyde condensation product sodium salt, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensation product, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, phosphate ester.

[0038] According to an embodiment of the present invention, the wetting agent is selected from any one, two or more of the following: trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium lauryl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkyl naphthalene sulfonate, sodium isooctyl succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether.

[0039] According to an embodiment of the present invention, the binder is selected from any one, two or more of the following: xanthan gum, starch, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, polyvinyl alcohol.

[0040] According to an embodiment of the present invention, the disintegrant is selected from any one, two or more of the following: sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, magnesium chloride.

[0041] According to an embodiment of the present invention, the thickener is selected from any one, two or more of the following: magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, polyvinyl alcohol.

[0042] According to an embodiment of the present invention, the defoaming agent is selected from any one, two or more of the following: silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate.

[0043] According to an embodiment of the present invention, the antifreeze agent is selected from any one, two or more of the following: propylene glycol, ethylene glycol, glycerol.

[0044] According to an embodiment of the present invention, the stabilizer is selected from any one, two or more of the following: triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylene diamine, and dialkyl succinic acid acetate sulfonate.

[0045] According to an embodiment of the present invention, the filler includes a solid filler and a liquid filler; preferably, the solid filler is selected from any one, two or more of the following: kaolin, attapulgite, diatomaceous earth, white carbon black, bentonite, montmorillonite, calcium carbonate, talc. Preferably, the liquid filler is selected from any one, two or more of the following: soybean oil, castor oil, mineral oil.

[0046] According to an embodiment of the present invention, the organic solvent is selected from any one, two or more of the following: ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, solvent oil (such as 150# solvent oil).

[0047] The present invention also provides use of the bactericidal composition for preventing and controlling pathogenic bacteria and agricultural diseases caused by them.

[0048] According to an embodiment of the present invention, the bactericidal composition is used to control bacteria and bacterial plant diseases caused by bacteria. According to an embodiment of the present invention, the bactericidal composition is used for:

[0049] (1) Control of soft rot of crops (e.g. soft rot of Chinese cabbage), black rot of Chinese cabbage, bacterial angular spot of cucumber, angular spot of sesame, fruit spot of watermelon, fruit spot of melon, bacterial leaf blight of rice, bacterial streak of rice, bacterial brown spot of rice, bacterial brown streak of rice, bacterial basal rot of rice, bacterial wilt of Solanaceae (e.g. bacterial wilt of tomato), bacterial wilt of mulberry, bacterial wilt of peanut, ginger blight, bacterial spot of tomato, bacterial spot of pepper, bacterial leaf spot of pepper, black leg of potato, bacterial wilt of corn , corn bacterial stem rot, wheat black glumes, soybean bacterial spot disease, soybean bacterial blight, cassava bacterial wilt, mango angular spot disease, citrus canker, peach bacterial punch hole disease, sunflower stem rot, peach gum disease, pear fire blight, pear rust water disease, tobacco wildfire disease, fruit tree bacterial root cancer, potato ring rot, bean wilt, wheat white leaf streak, potato scab, tomato canker, American holly leaf blight, pitaya bacterial scab and Panax notoginseng bacterial root rot and the pathogenic bacteria causing the above diseases;

[0050] (2) Prevent and control cucumber downy mildew, cucumber target spot disease, rice blast, rice sheath blight, tomato virus disease, and the pathogenic fungi and viruses that cause the above diseases.

[0051] According to an embodiment of the present invention, the fungicidal composition is used to prevent and control the following pathogens and the diseases caused by them: bacterial angular leaf spot of cucumber, bacterial leaf blight of rice, bacterial leaf streak of rice, citrus canker and other pathogens.

[0052] The present invention also provides a method for preventing and controlling pathogenic bacteria and agricultural diseases caused by them, the method comprising applying the fungicide composition to plants with diseases.

[0053] According to an embodiment of the present invention, the method is for preventing and controlling bacteria and bacterial plant diseases caused by bacteria. According to an embodiment of the present invention, the pathogenic bacteria are selected from: Cucumber bacterial angular leaf spot fungus, Rice bacterial leaf blight fungus, Rice bacterial leaf streak fungus, Citrus citri ulcer fungus.

[0054] The fungicidal composition of the present invention can be provided in the form of a finished preparation or a single dose, which can be directly mixed before use, and then mixed with water to a desired concentration, and can be applied to plants or crops in any manner, such as spraying, root irrigation, and painting. In specific applications, it can also be mixed with other agents such as insecticides, growth regulators, soil regulators, herbicides, nematicides, etc.

[0055] Beneficial effects of the present invention:

[0056] The present invention provides a synergistic fungicidal composition, which has the following advantages:

[0057] 1) It has a synergistic effect, which can improve the prevention and control effect and has a long lasting effect; 2) It expands the fungicide spectrum, and the field diseases often occur in a mixed manner, and the effect on bacterial diseases is stronger; 3) The two active ingredients have different mechanisms of action, and mixed use can delay the development of pathogen resistance; 4) It reduces the amount of pesticide applied, reduces the number of uses, and reduces the cost of use. DETAILED DESCRIPTION

[0058] The present invention will be described in further detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only exemplary descriptions and explanations of the present invention and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are included in the scope that the present invention is intended to protect.

[0059] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the reagents, materials, etc. used in the following examples, unless otherwise specified, can be obtained from commercial channels.

[0060] The present invention uses a combination of indoor bioassay and field test to test the bactericidal effect of the bactericidal composition. It should be noted that any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

[0061] 1. Preparation Example

[0062] Component I is: 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid ethyl ester; its structural formula is Component II is selected from: fluopicolide, pleuromutilin, chloroacetyl pleuromutilin, or fatty alcohol polyoxyethylene ether zinc carboxylate. The structural formula of fluopicolide is The structural formula of pleuromutilin is The structural formula of chloroacetyl pleuromutilin is The structural formula of fatty alcohol polyoxyethylene ether zinc carboxylate is Zn[C 12 H 25 O(CH2CH2O)9CH2COO]2.

[0063] Preparation Example 1: Wettable Powder

[0064]

[0065] The above materials are roughly crushed according to proportion, put into a mixer and mixed evenly, and then air flow crushed to obtain the finished product.

[0066] Preparation Example 2: Water Dispersible Granules

[0067]

[0068]

[0069] The effective active ingredients and various auxiliary agents are mixed evenly according to the formula ratio, and the powder is obtained after air flow crushing. A certain amount of water is added to mix and extrude granules, and the finished product is obtained after drying and screening.

[0070] Preparation Example 3: Suspension

[0071]

[0072] The effective active ingredients and various additives are mixed evenly according to the formula ratio, and the finished product is obtained after high-speed shearing and sand grinding.

[0073] Preparation Example 4: Emulsifiable Concentrate

[0074]

[0075] The above raw materials are mixed in proportion and dissolved into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0076] Preparation Example 5: Emulsion in water

[0077]

[0078]

[0079] According to the formula requirements, the above raw materials are added into the batching kettle, mixed evenly by a high-speed shearing machine to form a water emulsion, and after passing the inspection, they are measured and packaged to obtain the finished product.

[0080] Preparation Example 6: Emulsifiable Concentrate

[0081]

[0082] The above raw materials are mixed in proportion and dissolved into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0083] Preparation Example 7: Emulsifiable Concentrate

[0084]

[0085] The above raw materials are mixed in proportion and dissolved into a uniform oil phase; after passing the inspection, they are measured and packaged to obtain the finished product.

[0086] Preparation Example 8: Granules

[0087]

[0088]

[0089] The components are fully mixed according to the formula ratio, crushed, moistened with water and then fully stirred, and then granulated with a screw extrusion granulator, dried and sieved to obtain the finished product.

[0090] 2. Indoor Toxicity Determination Example

[0091] (1) Test pathogens

[0092] Bacterial diseases such as cucumber bacterial angular leaf spot, rice white leaf blight, rice bacterial leaf streak, and citrus canker.

[0093] (2) Measurement method

[0094] Bacterial toxicity determination method: The pathogenic bacteria toxicity determination uses bacterial diseases such as cucumber bacterial angular leaf spot, rice white leaf blight, rice bacterial leaf streak and citrus canker as test materials, and the in vitro turbidity method is used to determine the toxicity of component I (compound I), component II and their mixed preparations.

[0095] Referring to the agricultural industry standard of the People's Republic of China NY / T 1156.16-2008, the test reagent (including component I and component II) was first prepared into 7 concentration gradients (component I: 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 4.0μg / mL, 8.0μg / mL, 16.0μg / mL, 32.0μg / mL and other 7 concentration gradients; component II: 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 4.0μg / mL, 8.0μg / mL, 16.0μg / mL, 32.0μg / mL and other 7 concentration gradients) with a suitable solvent (the type of solvent is acetone, methanol, N,N dimethylformamide and dimethyl sulfoxide, etc., and is selected according to its solubility in the sample). Component I and component II for mixing were respectively adjusted according to their EC 50 A series of ratios were set according to the mass ratio, and the final mass concentration (referring to the total mass of component I and component II) was prepared into 7 series of concentrations of 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 4.0μg / mL, 8.0μg / mL, 16.0μg / mL, and 32.0μg / mL according to different ratios.

[0096] Under aseptic operation conditions, NB (nutrient broth, containing 10g of peptone, 3g of beef extract powder, and 5g of sodium chloride per liter) culture solution was dispensed into test tubes in equal amounts, and the drug solution was quantitatively drawn from low concentration to high concentration, added to the above test tubes respectively, and shaken thoroughly, and then an equal amount of bacterial suspension in the logarithmic growth phase was added respectively, and each treatment was repeated 4 times. After mixing, it was placed in a 25°C shaking incubator for dark culture, and the OD value was measured during the logarithmic growth phase. The average value of the four effective repetitions was taken as the measurement result, and the relative inhibition rate was calculated. The inhibition rate was converted into a probability value (y), and the drug solution concentration (μg / mL) was converted into a logarithmic value (x). The toxicity regression equation (y=a+bx) was obtained by the least squares method, and the EC of each agent was calculated from it. 50 At the same time, the synergistic ratio (SR) of the two drugs in different ratios was calculated according to the Wadley method. SR < 0.5 is antagonistic, 0.5 ≤ SR ≤ 1.5 is additive, and SR > 1.5 is synergistic. The calculation formula is as follows:

[0097] SR=EC 50 (Theoretical value) / EC 50 (Actual value)

[0098] EC 50 (theoretical value) = (a + b) / [(a / EC of A 50 )+(b / B's EC 50 )

[0099] In the formula: A is component I, B is component II; a and b are the proportions of component I and component II in the bactericidal composition, respectively;

[0100] (3) Measurement results

[0101] Example 2-1 Antibacterial effect of a mixture of compound I and pentocyclam against bacterial angular leaf spot of cucumber

[0102] In order to verify the antibacterial effect of different ratios of compound I (component I) and fluopicolide on bacterial angular leaf spot of cucumber, the indoor toxicity test of different ratios of compound I and fluopicolide on bacterial angular leaf spot of cucumber was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and the indoor antibacterial test was carried out. The test results are shown in Table 1.

[0103] Table 1 Results of toxicity test of compound I and fluopicolide alone and their mixtures against bacterial angular leaf spot of cucumber

[0104]

[0105] From the results in Table 1, it can be seen that the mixture of compound I and fluopicolide in the range of 50:1 to 1:50 has a significant synergistic effect on the inhibition of cucumber bacterial angular leaf spot. Compound I and fluopicolide in the ratio of 60:1 and 1:60 only have an additive effect rather than a synergistic effect on cucumber bacterial angular leaf spot.

[0106] Example 2-2 Antibacterial effect of a mixture of compound I and fluopicolide on citrus canker bacteria

[0107] In order to verify the antibacterial effect of different ratios of compound I and fluopicolide on citrus canker bacteria, indoor toxicity determination of different ratios of compound I and fluopicolide on citrus canker bacteria was carried out. The corresponding concentrations were prepared according to the weight percentages in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 2.

[0108] Table 2 Results of toxicity test of compound I and fluopicolide alone and their mixtures against citrus canker pathogen

[0109]

[0110]

[0111] From the results in Table 2, it can be seen that the mixture of compound I and fluopicolide in different ratios and dosages has a significant synergistic effect on the inhibition of citrus canker fungus.

[0112] Example 2-3 Antibacterial effect of a mixture of compound I and pentocyclanil on Xanthomonas oryzae

[0113] In order to verify the antibacterial effect of different ratios of compound I and fluopicolide on rice bacterial blight pathogen, indoor toxicity determination of compound I and fluopicolide in different ratios on rice bacterial blight pathogen was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 3.

[0114] Table 3 Results of toxicity test of compound I and fluopicolide alone and their mixtures against Xanthomonas oryzae

[0115]

[0116]

[0117] From the results in Table 3, it can be seen that the mixture of component I and fluopicolide in different ratios and dosages has a significant synergistic effect on the inhibition of rice bacterial blight pathogen.

[0118] Example 2-4 Antibacterial effect of the mixture of component I and chloroacetyl pleuromutilin on rice bacterial blight pathogen

[0119] In order to verify the antibacterial effect of different ratios of component I and chloroacetyl pleuromutilin on rice bacterial blight pathogen, indoor toxicity determination of component I and chloroacetyl pleuromutilin in different ratios on rice bacterial blight pathogen was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 4.

[0120] Table 4 The results of toxicity test of component I and chloroacetyl pleuromutilin single agent and mixture against Xanthomonas oryzae

[0121]

[0122] From the results in Table 4, it can be seen that the mixture of compound I and chloroacetyl pleuromutilin in the range of 50:1 to 1:50 has a significant synergistic effect on the inhibition of rice bacterial blight pathogen. Compound I and chloroacetyl pleuromutilin in the ratio of 60:1 and 1:60 only have an additive effect rather than a synergistic effect on rice bacterial blight pathogen.

[0123] Example 2-5 Antibacterial effect of a mixture of compound I and chloroacetyl pleuromutilin on rice bacterial leaf streak pathogen

[0124] In order to verify the antibacterial effect of different ratios of compound I and chloroacetyl pleuromutilin on rice bacterial leaf streak fungus, indoor toxicity determination of different ratios of compound I and chloroacetyl pleuromutilin on rice bacterial leaf streak fungus was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 5.

[0125] Table 5 The results of toxicity test of compound I and chloroacetyl pleuromutilin alone and their mixtures against rice bacterial leaf streak pathogen

[0126]

[0127] From the results in Table 5, it can be seen that the mixture of compound I and chloroacetylpleuromutilin in different ratios and dosages has a significant synergistic effect on the inhibition of rice bacterial leaf streak fungus.

[0128] Example 2-6 Antibacterial effect of a mixture of compound I and pleuromutilin on Xanthomonas oryzae

[0129] In order to verify the antibacterial effect of different ratios of compound I and pleuromutilin on rice bacterial blight pathogen, indoor toxicity determination of different ratios of compound I and pleuromutilin on rice bacterial blight pathogen was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 6.

[0130] Table 6 Results of toxicity test of compound I and pleuromutilin alone and their mixtures against Xanthomonas oryzae

[0131]

[0132] As can be seen from the above table, the mixture of compound I and pleuromutilin in the range of 50:1 to 1:50 has a significant synergistic effect on the inhibition of rice bacterial blight pathogen, and compound I and pleuromutilin in the ratio of 60:1 and 1:60 only have an additive effect rather than a synergistic effect on rice bacterial blight pathogen.

[0133] Example 2-7 Antibacterial effect of a mixture of compound I and pleuromutilin on bacterial leaf streak pathogen of rice

[0134] In order to verify the antibacterial effect of different ratios of compound I and pleuromutilin on rice bacterial leaf streak pathogen, indoor toxicity determination of compound I and pleuromutilin in different ratios on rice bacterial leaf streak pathogen was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 7.

[0135] Table 7 Results of toxicity test of compound I and pleuromutilin alone and their mixtures against bacterial leaf streak pathogen of rice

[0136]

[0137]

[0138] It can be seen from the above table that compound I, pleuromutilin and their mixed preparations all have a significant synergistic effect on the inhibition of rice bacterial leaf streak pathogen.

[0139] Example 2-8 Antibacterial effect of a mixture of compound I and fatty alcohol polyoxyethylene ether carboxylic acid zinc on rice bacterial leaf streak pathogen

[0140] In order to verify the antibacterial effect of different ratios of Compound I and fatty alcohol polyoxyethylene ether zinc carboxylate on rice bacterial leaf streak fungus, indoor toxicity determination of different ratios of Compound I and fatty alcohol polyoxyethylene ether zinc carboxylate on rice bacterial leaf streak fungus was carried out. The corresponding concentrations were prepared according to the weight percentage in the table, and indoor antibacterial tests were carried out. The test results are shown in Table 8.

[0141] Table 8 Results of toxicity test of compound I and fatty alcohol polyoxyethylene ether carboxylic acid zinc single agent and mixture against rice bacterial leaf streak pathogen

[0142]

[0143]

[0144] As can be seen from the above table, the mixture of compound I and fatty alcohol polyoxyethylene ether zinc carboxylate in the range of 50:1 to 1:50 has a significant synergistic effect on the inhibition of rice bacterial leaf streak disease. Compound I and fatty alcohol polyoxyethylene ether zinc carboxylate in the ratio of 60:1 and 1:60 only have an additive effect rather than a synergistic effect on rice bacterial leaf streak disease.

[0145] 3. Field efficacy embodiment

[0146] In order to clarify the efficacy of compound I and compound II alone and their mixtures on bacterial diseases such as cucumber bacterial angular leaf spot, citrus canker, rice white leaf blight, and rice bacterial leaf streak, multiple efficacy tests were carried out in different regions of the country.

[0147] The preparation methods of the reagents in the following examples are as shown in the above-mentioned preparation examples 1-8. For example, the preparation method of the 20% compound I·flutriazole suspension (1:1) in Example 3 is the same as that in Preparation Example 3, wherein compound II is selected from flutriazole; the preparation method of the 30% compound I·chloroacetyl pleuromutilin water dispersible granules (2:1) in Example 16 is the same as that in Preparation Example 2, wherein compound II is selected from chloroacetyl pleuromutilin; similarly, the preparation of other examples refers to the above-mentioned preparation examples 1-8.

[0148] The preparation methods of the reagents in the following comparative examples are the same as those in the above-mentioned preparation examples 1-8, except that the content of the active components therein may be different, and accordingly, the filler or water in the preparation makes up the components to 100%. For example, the preparation method of the 10% compound I suspension in comparative example 1 refers to preparation example 3, wherein 10% compound I replaces 10% compound I and 10% compound II in preparation example 3, and the difference in content is made up by the filler.

[0149] (1) Field efficacy test on bacterial angular leaf spot of cucumber (conducted in Qudi Town, Licheng District, Jinan, Shandong)

[0150] Test method: According to the application method specified in the National Standard of the People's Republic of China GB / T 17980.110-2004, apply the pesticide when the lesions first appear, and apply the pesticide for the second time 7 days later, for a total of two applications, repeated 4 times.

[0151] Investigation method: According to the investigation method specified in the National Standard of the People's Republic of China GB / T 17980.110-2004, the control effect was investigated 7 and 14 days after the second application of pesticides. Samples were taken at 3 points on the diagonal of each plot, with 5 plants at each point, and all leaves were investigated. The lesion area of ​​each leaf was graded as a percentage of the total leaf area.

[0152] Grading method:

[0153] Level 0: no lesions; Level 1: the lesion area accounts for less than 5% of the total leaf area; Level 3: the lesion area accounts for 6% to 10% of the total leaf area; Level 5: the lesion area accounts for 11% to 20% of the total leaf area; Level 7: the lesion area accounts for 21% to 50% of the total leaf area; Level 9: the lesion area accounts for more than 51% of the total leaf area.

[0154] Table 9 Field efficacy test of compound I combined with fluopicolide on bacterial angular leaf spot of cucumber

[0155]

[0156]

[0157] The test results in the table show that, when controlling bacterial angular leaf spot of cucumber, the compound I and fluopicolide combined with single agent have significantly higher 7-day and 14-day protective effects on bacterial angular leaf spot of cucumber than single agent at the same dosage of active ingredients. The 14-day protective effect can still reach more than 83.39%, with a longer duration.

[0158] (2) Field efficacy test on rice bacterial blight (conducted in Changzhou City, Jiangsu Province)

[0159] Test method: According to the application method specified in the National Standard of the People's Republic of China GB / T 17980.19-2000, apply the pesticide when the lesions first appear, and apply the pesticide for the second time 7 days later, for a total of two applications, repeated 4 times.

[0160] Investigation method: According to the investigation method specified in the National Standard of the People's Republic of China GB / T 17980.19-2000, the control effect was investigated 7 and 14 days after the second application of the pesticide.

[0161] Prevalence of bacterial blight (grading by visual inspection):

[0162] Level 0: no disease; Level 1: sporadic disease or central disease clusters; Level 3: the diseased area accounts for about one quarter of the total area; Level 5: the diseased area accounts for about one third of the total area; Level 7: the diseased area accounts for about one half of the total area; Level 9: the diseased area accounts for more than three quarters of the total area.

[0163] Table 10 Field efficacy test of compound I combined with pentocyclanil against rice bacterial blight

[0164]

[0165] The test results show that, when controlling rice bacterial leaf blight, the compound I and fluopicolide were compared with the single agent. At the same dosage of active ingredients, the 7-day and 14-day control effects of compound I and fluopicolide against rice bacterial leaf blight were significantly higher than those of the single agent. The 14-day control effect can still reach more than 82.12%, and the duration of effect is longer.

[0166] Table 11 Field efficacy test of compound I combined with chloroacetyl pleuromutilin against rice bacterial blight

[0167]

[0168] The test results show that, when controlling rice bacterial blight, the compound I and chloroacetyl pleuromutilin were compared with the single agent. At the same dosage of active ingredients, the 7-day and 14-day protective effects of the compound I + chloroacetyl pleuromutilin combination on rice bacterial blight were significantly higher than those of the single agent. The 14-day protective effect can still reach more than 82.20%, with a longer duration.

[0169] Table 12 Field efficacy test of compound I combined with pleuromutilin against rice bacterial blight

[0170]

[0171] The test results show that, when controlling rice bacterial blight, the compound I and pleuromutilin combination and single agent are compared. At the same dosage of active ingredients, the 7-day and 14-day protective effects of the compound I + pleuromutilin combination on rice bacterial blight are significantly higher than those of the single agent. The 14-day protective effect can still reach more than 85.25%, and the duration of effect is longer.

[0172] (3) Field efficacy test on citrus canker (conducted in Wuming, Guangxi)

[0173] According to the "Guidelines for Field Efficacy Tests of Pesticides (GB / T17980.103-2004)", the first spraying was done before flowering, the second spraying was done after the disease appeared, and the third spraying was done 7 days after the second spraying. The spraying was done three times in total, with each treatment area covering 3 mature fruit trees, and the number of repetitions was 4 times. The number of sprayings, the date of each spraying, and the growth period of the fruit trees were recorded.

[0174] Investigation method: According to the investigation method specified in the Guidelines for Field Efficacy Tests of Pesticides (GB / T17980.103-2004), two plants in each plot were investigated 7 and 14 days after the third application of pesticides. Samples were taken from each plant at five points in the east, west, south, north and center. All leaves on the two tips were investigated at each point to calculate the control efficacy.

[0175] Leaf Grading Method:

[0176] Level 0: disease-free; Level 1: 1 to 5 disease spots on each leaf; Level 3: 6 to 10 disease spots on each leaf; Level 5: 11 to 15 disease spots on each leaf; Level 7: 15 to 20 disease spots on each leaf; Level 9: more than 21 disease spots on each leaf.

[0177] Table 13 Field efficacy test of compound I combined with fluopicolide against citrus canker

[0178]

[0179] The test results show that when controlling citrus canker, the compound I and fluopicolide hydroxylamide combination and single agent have significantly higher 7-day and 14-day control effects on citrus canker than single agent at the same dosage of active ingredients. The 14-day control effect can still reach more than 83.38%, with a longer duration.

[0180] (4) Field efficacy test on rice bacterial leaf streak (conducted in Siyang County, Jiangsu Province)

[0181] Test method: According to the application method specified in the National Standard of the People's Republic of China GB / T 17980.105-2004, apply the pesticide when the lesions first appear, and apply the pesticide for the second time 7 days later, for a total of two applications, repeated 4 times.

[0182] Investigation method: According to the investigation method specified in the National Standard of the People's Republic of China GB / T 17980.105-2004, the control effect was investigated 7 and 14 days after the second application of the pesticide.

[0183] Prevalence of bacterial leaf streak (grading by visual inspection):

[0184] Level 0: no lesions on leaves; Level 1: only small translucent water-soaked lesions on leaves, accounting for less than 1% of the leaf area; Level 3: sporadic short and narrow lesions on leaves, accounting for 1% to 5% of the leaf area; Level 5: more lesions on leaves, accounting for 6% to 25% of the leaf area; Level 7: dense lesions on leaves, accounting for 26% to 50% of the leaf area; Level 9: dense lesions on leaves, accounting for more than 51% of the leaf area, the leaves turn orange-brown, curl, and die.

[0185] Table 14 Field efficacy test of compound I combined with pleuromutilin against rice bacterial leaf streak

[0186]

[0187]

[0188] Table 15 Field efficacy test of compound I combined with zinc fatty alcohol polyoxyethylene ether carboxylate against rice bacterial leaf streak

[0189]

[0190] Table 16 Field efficacy test of compound I combined with chloroacetyl pleuromutilin against rice bacterial leaf streak

[0191]

[0192]

[0193] It can be seen from the test results that in the prevention and treatment of bacterial leaf streak disease of rice, the combination of compound I with pleuromutilin, or zinc fatty alcohol polyoxyethylene ether carboxylate, or chloroacetyl pleuromutilin and the single agent are compared. At the same dosage of active ingredients, the 7-day and 14-day prevention effects of the combination on bacterial leaf streak disease of rice are significantly higher than those of the single agent, and the duration of effect is longer.

[0194] The above is an explanation of the embodiments of the present invention. However, the present invention is not limited to the above embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A fungicide composition, comprising effective active ingredients, wherein the effective active ingredients include component I and component II, wherein component I is selected from 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid ethyl ester, and component II is selected from any one, two or more of fluopicolide, pleuromutilin, chloroacetyl pleuromutilin, and fatty alcohol polyoxyethylene ether zinc carboxylate.

2. The bactericidal composition according to claim 1, characterized in that The structural formula of component I is The structural formula of fluopicolide is The structural formula of pleuromutilin is The structural formula of chloroacetyl pleuromutilin is The structural formula of fatty alcohol polyoxyethylene ether zinc carboxylate is: Zn[R(CH2CH2O)n CH2COO]2, wherein R is selected from C6-C14 fatty alcohol and its isomeric alcohol, and n is selected from a natural number of 3-9.

3. The bactericidal composition according to claim 1 or 2, characterized in that The fungicide composition comprises component I and fluopicolide; or, the fungicide composition comprises component I and pleuromutilin; or, the fungicide composition comprises component I and chloroacetyl pleuromutilin; or, the fungicide composition comprises component I and fatty alcohol polyoxyethylene ether zinc carboxylate; Preferably, the mass ratio of component I to component II is 80-1:1-80; preferably 50-1:1-50; Preferably, based on the total weight of the bactericidal composition being 100%, the sum of the weight of component I and component II in the bactericidal composition is 0.1% to 90%; Preferably, based on the total weight of the bactericidal composition being 100%, the mass of component I in the bactericidal composition is 0.1% to 80%; Preferably, based on the total weight of the bactericidal composition being 100%, the mass of component II in the bactericidal composition is 0.1% to 80%.

4. The bactericidal composition according to any one of claims 1 to 3, characterized in that The bactericidal composition can be prepared into a liquid preparation or a solid preparation; Preferably, the liquid preparation contains 0.1% to 60% by weight of the effective active ingredient, preferably 0.5% to 50%; Preferably, the solid preparation contains 0.5% to 80% by weight of the effective active ingredient, preferably 1% to 70%.

5. The bactericidal composition according to any one of claims 1 to 4, characterized in that The bactericidal composition may also optionally include any one, two or more of an emulsifier, a dispersant, a wetting agent, a thickener, a defoaming agent, a stabilizer, a binder, a disintegrant, an antifreeze agent, an anticaking agent, a suspending agent, a film former, a preservative, a colorant, a polymer capsule wall material, a pH regulator or a filler; And / or, the bactericidal composition may be diluted before use or used directly.

6. The bactericidal composition according to any one of claims 1 to 5, characterized in that The bactericidal composition can be prepared into various dosage forms; preferably, the dosage forms include water-dispersible granules, dispersible liquids, wettable powders, suspensions, aqueous emulsions, microemulsions, suspoemulsions, microcapsule suspensions, microcapsule suspension-suspension concentrates, suspension seed coatings, emulsifiable concentrates, and granules.

7. The bactericidal composition according to any one of claims 1 to 6, characterized in that When the bactericidal composition is made into a wettable powder, it comprises the following components and contents: component I 1% to 80%, component II 1% to 80%, dispersant 1% to 20%, wetting agent 1% to 8%, and the balance is filler; preferably, it comprises the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 5%, and the balance is filler; And / or, when the composition is made into water-dispersible granules, it comprises the following components and contents: component I 1% to 80%, component II 1% to 80%, dispersant 1% to 20%, wetting agent 1% to 8%, disintegrant 1% to 10%, and the balance is filler; preferably, it comprises the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 12%, wetting agent 1% to 5%, disintegrant 1% to 8%, and the balance is filler; And / or, when the composition is made into a suspension, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, dispersant 1% to 20%, wetting agent 1% to 10%, thickener 0.1% to 10%, antifreeze agent 0.1% to 8%, and the balance is water; preferably, it includes the following components and contents: component I 1% to 30%, component II 1% to 30%, dispersant 1% to 12%, wetting agent 1% to 8%, thickener 0.1% to 8%, antifreeze agent 0.1% to 8%, and the balance is water; And / or, when the composition is made into an aqueous emulsion, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, organic solvent 1% to 70%, emulsifier 1% to 20%, antifreeze 0.1% to 8%, defoamer 0.01% to 2%, thickener 0.1% to 2%, and the balance is water; preferably, it includes the following components and contents: component I 1% to 30%, component II 1% to 30%, organic solvent 10% to 60%, emulsifier 1% to 12%, antifreeze 0.1% to 5%, defoamer 0.01% to 1%, thickener 0.1% to 1%, and the balance is water; And / or, when the composition is made into a microcapsule suspension, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, polymer capsule wall material 1-30%, dispersant 2% to 10%, organic solvent 1 to 50%, emulsifier 1% to 7%, pH regulator 0.1% to 5%, defoamer 0.01% to 2%, thickener 0.1% to 8%, antifreeze agent 0.1% to 8%, and the balance is water; And / or, when the composition is made into an emulsifiable concentrate, it comprises the following components and contents: component I 1% to 50%, component II 1% to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is an organic solvent; preferably, it comprises the following components and contents: component I 1% to 40%, component II 1% to 30%, emulsifier 1 to 20%, antifreeze agent 1% to 8%, stabilizer 0.1% to 3%, and the balance is an organic solvent; And / or, when the composition is made into a microemulsion, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, organic solvent 1 to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is water; And / or, when the composition is made into a dispersible liquid, it includes the following components and contents: component I 1% to 50%, component II 1% to 50%, emulsifier 1 to 30%, antifreeze agent 1% to 10%, stabilizer 0.1% to 5%, and the balance is organic solvent; And / or, when the composition is made into granules, it includes the following components and contents: component I 0.5%-10%, component II 0.5%-10%, dispersant 1%-12%, wetting agent 1%-10%, binder 0.1%-8%, and the balance is filler; preferably, it includes the following components and contents: component I 0.5%-5%, component II 0.5%-5%, dispersant 1%-10%, wetting agent 1%-8%, binder 0.1%-5%, and the balance is filler.

8. The bactericidal composition according to claim 7, characterized in that The emulsifier is selected from any one, two or more of the following: sodium lignin sulfonate, agricultural milk, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan anhydride oleate, fatty alcohol polyoxyethylene ether, sodium alkylnaphthalene sulfonate, sodium isooctyl succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, castor oil polyoxyethylene ether phosphate; And / or, the dispersant is selected from any one, two or more of the following: glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignin sulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate, naphthalene sulfonic acid formaldehyde condensate sodium salt, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, phosphate ester; And / or, the wetting agent is selected from any one, two or more of the following: trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium lauryl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenyl ethyl phenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate, sodium isooctyl succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether; and / or, the binder is selected from any one, two or more of the following: xanthan gum, starch, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, polyvinyl alcohol; and / or, the disintegrant is selected from any one, two or more of the following: sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, magnesium chloride; and / or, the thickener is selected from any one, two or more of the following: magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, polyvinyl alcohol; And / or, the defoaming agent is selected from any one, two or more of the following: silicone oil, n-octanol, silicone, butyl phosphate, isobutyl phosphate; And / or, the antifreeze agent is selected from any one, two or more of the following: propylene glycol, ethylene glycol, glycerol; And / or, the stabilizer is selected from any one, two or more of the following: triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylene diamine, dialkyl succinic acid acetate sulfonate; And / or, the filler includes a solid filler and a liquid filler; preferably, the solid filler is selected from any one, two or more of the following: kaolin, attapulgite, diatomaceous earth, white carbon black, bentonite, montmorillonite, calcium carbonate, talc; preferably, the liquid filler is selected from any one, two or more of the following: soybean oil, castor oil, mineral oil; And / or, the organic solvent is selected from any one, two or more of the following: ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, and solvent oil.

9. Use of the bactericidal composition according to any one of claims 1 to 8 for preventing and controlling pathogenic bacteria and agricultural diseases caused by them; Preferably, the fungicidal composition is used to control bacteria and bacterial plant diseases caused by bacteria; Preferably, the bactericidal composition is used for: (1) Control crop soft rot, cabbage black rot, cucumber bacterial angular spot, sesame angular spot, watermelon fruit spot, melon fruit spot, rice bacterial leaf blight, rice bacterial streak, rice bacterial brown spot, rice bacterial brown streak, rice bacterial basal rot, solanaceous bacterial wilt, mulberry bacterial wilt, peanut bacterial wilt, ginger blight, tomato bacterial spot, pepper bacterial spot, pepper bacterial leaf spot, potato black shank, corn bacterial wilt, corn bacterial stalk rot, Wheat black glumes, soybean bacterial spot disease, soybean bacterial blight, cassava bacterial wilt, mango angular spot disease, citrus canker, peach bacterial punch hole disease, sunflower stem rot, peach gum disease, pear fire blight, pear rust water disease, tobacco wildfire disease, fruit tree bacterial root cancer, potato ring rot, bean wilt, wheat white leaf streak, potato scab, tomato canker, American holly leaf blight, pitaya bacterial scab and Panax notoginseng bacterial root rot, as well as the pathogenic bacteria that cause the above diseases; (2) Prevent and control cucumber downy mildew, cucumber target spot disease, rice blast, rice sheath blight, tomato virus disease, and the pathogenic fungi and viruses that cause the above diseases.

10. A method for preventing and controlling pathogenic bacteria and agricultural diseases caused by them, the method comprising applying the fungicidal composition according to any one of claims 1 to 8 to plants with diseases; Preferably, the method is for preventing and controlling bacteria and bacterial plant diseases caused by bacteria; Preferably, the pathogen is selected from: bacterial angular leaf spot of cucumber, bacterial leaf blight of oryzae, bacterial leaf streak of oryzae, and citrus canker.

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