Bactericide and method for preventing and treating radix bupleuri root rot

Abstract

The invention belongs to the technical field of disease control, and discloses a bactericide for controlling radix bupleuri root rot, which is prepared from the following components: 10%-20% of a vesicle-arbuscular mycorrhizal agent, 30%-50% of carbendazim, 15%-25% of JS399-19, 10%-20% of phenazino-1-carboxylic acid, 10%-20% of bacillus subtilis, 3%-7% of a surfactant, 3%-7% of a stabilizer and the balance of a carrier, the dispersing agent is used for diluting and dispersing the active ingredients. By scientifically proportioning various efficient active components such as the vesicle-arbuscular mycorrhizal agent, the carbendazim, the phenamacril, the phenazino-1-carboxylic acid and the bacillus subtilis, a set of multi-target and multi-layer disease prevention and control system is formed, pathogenic bacteria of radix bupleuri root rot are accurately struck, growth and reproduction of radix bupleuri root rot are effectively inhibited, and the disease incidence rate is greatly reduced. Compared with a traditional medicament, the bactericide not only has a remarkable control effect, but also is safe and friendly to human bodies, animals and the environment, the use frequency and the total amount of the medicament are reduced, the requirements of consumers on green agricultural products are met, and occurrence and spreading of radix bupleuri root rot are controlled.

Description

Technical Field

[0001] The invention belongs to the technical field of disease prevention and treatment, and specifically relates to a fungicide and a method for preventing and treating bupleurum root rot. Background Art

[0002] As an important Chinese medicinal material, Bupleurum has a vast market demand. However, the decreasing reserves of wild resources have made artificial cultivation an inevitable choice. However, with the continuous expansion of the artificial cultivation area of ​​Bupleurum, the problem of pests and diseases has become increasingly serious, among which root rot has become the most prominent disease. Bupleurum root rot is mainly caused by Fusarium oxysporum and Fusarium solani. These pathogens are particularly active in hot and rainy seasons, mainly harming the roots of Bupleurum, causing root rot, which in turn affects the yield and quality of Bupleurum.

[0003] Most of the existing prevention and control methods use chemical treatment. The method of preventing and controlling Bupleurum root rot is mainly to use chemical treatment when the disease is serious. This method has the following defects:

[0004] Abuse of pesticides: Due to the delayed timing of prevention and control, pesticides often need to be applied only when the disease has already caused serious losses, resulting in excessive use of pesticides and abuse.

[0005] Pesticide residues exceed the standard: Residues of chemical agents may pose a threat to the quality and safety of Bupleurum chinense, and there is a risk of pesticide residues exceeding the standard.

[0006] Low prevention effect: Long-term use of chemical agents can easily lead to drug resistance in pathogens, which gradually reduces the prevention and control effect.

[0007] Environmental pollution: The loss and residue of chemical agents may also pollute the soil and water, affecting the ecological environment.

[0008] Therefore, in response to the above problems in preventing and treating Bupleurum root rot, the present application proposes to develop a new fungicide that is highly efficient, low-toxic, environmentally friendly and not prone to drug resistance, which is of great significance for ensuring the sustainable development of Bupleurum cultivation. Summary of the invention

[0009] The object of the present invention is to provide a fungicide and method for preventing and treating Bupleurum root rot, so as to solve the problems raised in the above background technology.

[0010] In order to achieve the above-mentioned purpose, the present invention provides the following technical scheme: a fungicide for preventing and treating Bupleurum root rot, which is made of the following components: vesicle-arbuscular mycorrhizal agent: 10%-20%, carbendazim: 30%-50%, cyanocobalamin: 15%-25%, sinocycline: 10%-20%, Bacillus subtilis: 10%-20%, surfactant: 3%-7%, stabilizer: 3%-7%, carrier: the balance, which is used to dilute and disperse the above-mentioned active ingredients.

[0011] Preferably, the fungicide is made of the following components: 15% vesicle-arbuscular mycorrhizal agent, 30% carbendazim, 15% cyanoacrylate, 15% succinimide, 15% bacillus subtilis, 10% surfactant, 5% stabilizer, and 5% carrier.

[0012] Preferably, the carrier is but not limited to diatomaceous earth, kaolin or a suitable inert carrier material.

[0013] Preferably, the preparation steps include:

[0014] Step 1, weighing each component according to the ratio described in claim 1;

[0015] Step 2, uniformly mixing vesicle-arbuscular bacteria, carbendazim, cyanocobalamin, Bacillus subtilis and sinocycline;

[0016] Step 3, adding surfactant and continuing to mix until uniform;

[0017] Step 4: Add stabilizer and mix well again;

[0018] Step five, finally add the carrier, mix thoroughly, and obtain the fungicide.

[0019] Preferably, the method steps are as follows:

[0020] Step 1: Seed coating: Before sowing, soak the Bupleurum seeds in warm water until they are saturated with water, then soak and disinfect them in the diluted solution of the fungicide for 15-30 minutes, and sow them after drying, which helps to reduce the number of pathogens carried by the seeds and reduce the incidence of diseases;

[0021] Step 2, soil treatment: before planting Bupleurum, disinfect the soil; mix the fungicide with fine sand or dry soil in a ratio of 1:20-30, spread it in the field, and then plow the soil to make the agent evenly distributed, which helps to kill pathogens in the soil and reduce the source of disease transmission; before planting, apply a base fertilizer composed of a large number of elements and biological organic fertilizer;

[0022] Step 3: irrigate the roots with the drug; in the early stage of Bupleurum disease, use fungicide for root irrigation; dilute the fungicide to an appropriate concentration and evenly irrigate the soil around the roots of Bupleurum. The amount of irrigation per plant depends on the size of the plant, generally 50-100 ml; root irrigation is carried out every 7-10 days, for 2-3 times in a row, which helps to directly kill the root pathogens and control the development of the disease;

[0023] Step 4, field management: Cut the stems of Bupleurum chinense twice before buds appear, and spray foliar fertilizer and plant-based fungicide after the second cutting.

[0024] Preferably, the concentration of the fungicide dilution solution in the seed coating in step one and the root irrigation in step three is 500-1000 times for the best effect.

[0025] Preferably, the weight ratio of the macroelements to the bio-organic fertilizer is 1:5 to 1:6; the macroelements include potassium sulfate type compound fertilizer; and the bio-organic fertilizer includes microbial agents and organic fertilizer.

[0026] Preferably, the biological organic fertilizer comprises 3-5 parts of microbial agent, 1-2.5 parts of brown sugar, 30-40 parts of wheat bran, 45-65 parts of bentonite and 120-180 parts of decomposed sawdust; the microbial agent is composed of 1-2 parts of lactic acid bacteria, 1-2 parts of Bacillus megaterium and 1-2 parts of Metarhizium anisopliae.

[0027] Preferably, the preparation method of the bio-organic fertilizer is:

[0028] Step 1: Nutrition Preparation of liquid: Add brown sugar to 10 times warm water and dissolve it. Stir continuously during the dissolution process to make the added brown sugar completely dissolved.

[0029] Step 2: pretreatment of the microbial agent: before sprinkling into the brown sugar water, premix the microbial agent and the wheat bran to ensure that the microbial agent is evenly distributed on the bran so that it can be more evenly dissolved and fermented when sprinkled into the brown sugar water;

[0030] Step 3: Seal the mouth of the barrel with a sealing film, use a constant temperature fermentation box for mixed fermentation for 48 hours, control the temperature between 25-35°C, uncover the sealing film once every 24 hours to allow the gas to escape, and seal the mouth of the barrel again;

[0031] Step 4: Mix the prepared bentonite and decomposed sawdust evenly and spread them flatly with a thickness of 55-58cm;

[0032] Step 5: Sprinkle the prepared fermentation bacteria agent onto the spread bentonite and decomposed wood chips and mix them evenly to obtain the biological organic fertilizer.

[0033] Preferably, the stem cutting time is from mid-June to mid-July of the second year before the first sequence of flowers of Bupleurum chinense buds, and the first stem cutting operation is performed. One month later, when the second sequence of flowers buds, the second stem cutting treatment is performed; the stubble height of the two stem cuttings is 18 cm.

[0034] The beneficial effects of the present invention are as follows:

[0035] 1. The present invention forms a multi-target, multi-level disease prevention and control system by scientifically proportioning a variety of highly effective active ingredients such as vesicle-arbuscular mycorrhizal agents, carbendazim, cyanocobalamin, sinoprolin and Bacillus subtilis. These ingredients work synergistically to accurately attack the pathogens of Bupleurum root rot, effectively inhibit their growth, reproduction and spread, thereby greatly reducing the incidence of the disease and ensuring the healthy growth of Bupleurum. Compared with traditional single agents, this fungicide not only has a more significant prevention and control effect, but also reduces the frequency and total amount of agent use, reduces the risk of drug resistance of pathogens, and extends the service life of the agent.

[0036] 2. The present invention uses active ingredients that are mostly biological or low-toxic chemical preparations, which show good safety to humans, animals and the environment. During the application process, it will not pollute the environment, nor will it destroy the ecological balance of soil microorganisms, which is conducive to maintaining ecological balance and biodiversity. In addition, by reducing the use of chemical pesticides, this fungicide helps to reduce pesticide residues, improve the safety and quality of Bupleurum products, and meet consumers' demand for green and organic agricultural products. The new fungicide components and ratios developed in this project are characterized by high efficiency, low toxicity, environmental friendliness, and not easy to induce drug resistance. The method combined with microbial control methods has the advantages of low cost, good effect, and little pollution to the environment, and is expected to effectively control the occurrence and spread of Bupleurum root rot.

[0037] 3. The method of using the fungicide of the present invention is simple and clear, easy to operate, and can reduce the amount and frequency of pesticide use, thereby reducing production costs and improving the yield and quality of Bupleurum. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 The present invention is a simplified flow chart of the method for preparing the fungicide;

[0039] Figure 2 The present invention is a simplified flowchart of the steps of the method for using the fungicide. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] like Figure 1 to Figure 2 As shown, an embodiment of the present invention provides a fungicide for preventing and treating Bupleurum root rot, which is made of the following components: vesicle-arbuscular mycorrhizal agent: 10%-20%, carbendazim: 30%-50%, cyanocobalamin: 15%-25%, sinocycline: 10%-20%, Bacillus subtilis: 10%-20%, surfactant: 3%-7%, stabilizer: 3%-7%, carrier: the balance, which is used to dilute and disperse the above active ingredients.

[0042] The fungicide is prepared from the following components: 15% of vesicle-arbuscular mycorrhizal agent, 30% of carbendazim, 15% of cyanocobalamin, 15% of succinic acid, 10% of bacillus subtilis, 5% of surfactant, 5% of stabilizer and 5% of carrier.

[0043] The carrier is, but not limited to, diatomaceous earth, kaolin or a suitable inert carrier material.

[0044] The preparation steps include:

[0045] Step 1, weighing each component according to the ratio of claim 1;

[0046] Step 2, uniformly mixing vesicle-arbuscular bacteria, carbendazim, cyanocobalamin, Bacillus subtilis and sinocycline;

[0047] Step 3, adding surfactant and continuing to mix until uniform;

[0048] Step 4: Add stabilizer and mix well again;

[0049] Step five, finally add the carrier, mix thoroughly, and obtain the fungicide.

[0050] The usage steps are as follows:

[0051] Step 1: Seed coating: Before sowing, soak the Bupleurum seeds in warm water until they are saturated with water, then soak and disinfect them in the diluted solution of the fungicide for 15-30 minutes, and sow them after drying, which helps to reduce the number of pathogens carried by the seeds and reduce the incidence of diseases;

[0052] Step 2, soil treatment: before planting Bupleurum, disinfect the soil; mix the fungicide with fine sand or dry soil in a ratio of 1:20-30, spread it in the field, and then plow the soil to make the agent evenly distributed, which helps to kill pathogens in the soil and reduce the source of disease transmission; before planting, apply a base fertilizer composed of a large number of elements and biological organic fertilizer;

[0053] Step 3: irrigate the roots with the drug; in the early stage of Bupleurum disease, use fungicide for root irrigation; dilute the fungicide to an appropriate concentration and evenly irrigate the soil around the roots of Bupleurum. The amount of irrigation per plant depends on the size of the plant, generally 50-100 ml; root irrigation is carried out every 7-10 days, for 2-3 times in a row, which helps to directly kill the root pathogens and control the development of the disease;

[0054] Step 4, field management: Cut the stems of Bupleurum chinense twice before buds appear, and spray foliar fertilizer and plant-based fungicide after the second cutting.

[0055] Among them, the best effect is achieved when the concentration of the fungicide diluted solution in step one, seed coating, and step three, root irrigation, is 500-1000 times.

[0056] Among them, the weight ratio of macroelements to bio-organic fertilizer is 1:5-1:6; the macroelements include potassium sulfate type compound fertilizer; and the bio-organic fertilizer includes microbial agents and organic fertilizer.

[0057] Among them, the biological organic fertilizer includes 3-5 parts of microbial agents, 1-2.5 parts of brown sugar, 30-40 parts of wheat bran, 45-65 parts of bentonite and 120-180 parts of decomposed sawdust; the microbial agent is composed of 1-2 parts of lactic acid bacteria, 1-2 parts of Bacillus megaterium and 1-2 parts of Metarhizium muscardine.

[0058] Wherein, the preparation method of biological organic fertilizer is:

[0059] Step 1: Nutrition Preparation of liquid: Add brown sugar to 10 times warm water and dissolve it. Stir continuously during the dissolution process to make the added brown sugar completely dissolved.

[0060] Step 2: pretreatment of the microbial agent: before sprinkling into the brown sugar water, premix the microbial agent and the wheat bran to ensure that the microbial agent is evenly distributed on the bran so that it can be more evenly dissolved and fermented when sprinkled into the brown sugar water;

[0061] Step 3: Seal the mouth of the barrel with a sealing film, use a constant temperature fermentation box for mixed fermentation for 48 hours, control the temperature between 25-35°C, uncover the sealing film once every 24 hours to allow the gas to escape, and seal the mouth of the barrel again;

[0062] Step 4: Mix the prepared bentonite and decomposed sawdust evenly and spread them flatly with a thickness of 55-58cm;

[0063] Step 5: Sprinkle the prepared fermentation bacteria agent onto the spread bentonite and decomposed wood chips and mix them evenly to obtain biological organic fertilizer.

[0064] The first stem cutting operation was carried out from mid-June to mid-July of the second year before the first flower of Bupleurum chinense budded. The second stem cutting was carried out one month later when the second flower budded. The stubble height of the two stem cuttings was 18 cm.

[0065] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fungicide for preventing and treating Bupleurum root rot, characterized in that: The fungicide is prepared from the following components: vesicle-arbuscular mycorrhizal agent: 10%-20%, carbendazim: 30%-50%, cyanocobalamin: 15%-25%, sinocycline: 10%-20%, bacillus subtilis: 10%-20%, surfactant: 3%-7%, stabilizer: 3%-7% and carrier: the balance is used for diluting and dispersing the above active ingredients.

2. A fungicide for preventing and treating Bupleurum root rot according to claim 1, characterized in that: The bactericide is prepared from the following components: 15% of vesicle-arbuscular mycorrhizal agent, 30% of carbendazim, 15% of cyanobacterium methyl, 15% of succinimide, 10% of bacillus subtilis, 5% of surfactant, 5% of stabilizer and 5% of carrier.

3. A fungicide for preventing and treating Bupleurum root rot according to claim 1, characterized in that: The carrier may be, but is not limited to, diatomaceous earth, kaolin or a suitable inert carrier material.

4. A method for preparing a fungicide for preventing and treating Bupleurum root rot according to claims 1-3, characterized in that: The preparation steps include: Step 1, weighing each component according to the ratio of claim 1; Step 2, uniformly mixing vesicle-arbuscular bacteria, carbendazim, cyanocobalamin, Bacillus subtilis and sinocycline; Step 3, adding surfactant and continuing to mix until uniform; Step 4: Add stabilizer and mix well again; Step five, finally add the carrier, mix thoroughly, and obtain the fungicide.

5. A method for using a fungicide for preventing and treating Bupleurum root rot according to claims 1-3, characterized in that: The steps for use are as follows: Step 1: Seed coating: Before sowing, soak the Bupleurum seeds in warm water until they are saturated with water, then soak and disinfect them in the diluted solution of the fungicide for 15-30 minutes, and sow them after drying, which helps to reduce the number of pathogens carried by the seeds and reduce the incidence of diseases; Step 2: Soil treatment: Before planting Bupleurum chinense, disinfect the soil; Mix the fungicide with fine sand or dry soil at a ratio of 1:20-30, spread it on the field, and then till the soil to evenly distribute the agent, which helps to kill pathogens in the soil and reduce the source of disease transmission; apply base fertilizer composed of macroelements and biological organic fertilizer before planting; Step 3: irrigate the roots with the drug; in the early stage of Bupleurum disease, use fungicide for root irrigation; dilute the fungicide to an appropriate concentration and evenly irrigate the soil around the roots of Bupleurum. The amount of irrigation per plant depends on the size of the plant, generally 50-100 ml; root irrigation is carried out every 7-10 days, for 2-3 times in a row, which helps to directly kill the root pathogens and control the development of the disease; Step 4, field management: Cut the stems of Bupleurum chinense twice before buds appear, and spray foliar fertilizer and plant-based fungicide after the second cutting.

6. The method for using a fungicide for preventing and treating Bupleurum root rot according to claim 5, characterized in that: The best effect is achieved when the concentration of the fungicide dilution solution in the seed coating step one and the root irrigation step three is 500-1000 times.

7. The method for using a fungicide for preventing and treating Bupleurum root rot according to claim 5, characterized in that: The weight ratio of the macroelements to the bio-organic fertilizer is 1:5-1:6; the macroelements include potassium sulfate type compound fertilizer; and the bio-organic fertilizer includes microbial agents and organic fertilizer.

8. The method for using a fungicide for preventing and treating Bupleurum root rot according to claim 5, characterized in that: The biological organic fertilizer comprises 3-5 parts of microbial agent, 1-2.5 parts of brown sugar, 30-40 parts of wheat bran, 45-65 parts of bentonite and 120-180 parts of decomposed sawdust; the microbial agent is composed of 1-2 parts of lactic acid bacteria, 1-2 parts of Bacillus megaterium and 1-2 parts of Metarhizium anisopliae.

9. The method for using a fungicide for preventing and treating Bupleurum root rot according to claim 5, characterized in that: The preparation method of the biological organic fertilizer is: Step 1: Nutrition Preparation of liquid: Add brown sugar to 10 times warm water and dissolve it. Stir continuously during the dissolution process to make the added brown sugar completely dissolved. Step 2: pretreatment of the microbial agent: before sprinkling into the brown sugar water, premix the microbial agent and the wheat bran to ensure that the microbial agent is evenly distributed on the bran so that it can be more evenly dissolved and fermented when sprinkled into the brown sugar water; Step 3: Seal the mouth of the barrel with a sealing film, use a constant temperature fermentation box for mixed fermentation for 48 hours, control the temperature between 25-35°C, uncover the sealing film once every 24 hours to allow the gas to escape, and seal the mouth of the barrel again; Step 4: Mix the prepared bentonite and decomposed sawdust evenly and spread them flatly with a thickness of 55-58cm; Step 5: Sprinkle the prepared fermentation bacteria agent onto the spread bentonite and decomposed wood chips and mix them evenly to obtain the biological organic fertilizer.

10. The method for using a fungicide for preventing and treating Bupleurum root rot according to claim 5, characterized in that: The stem cutting time is from mid-June to mid-July of the second year before the first flower of Bupleurum chinense buds, and the first stem cutting operation is carried out. One month later, when the second flower of Bupleurum chinense buds, the second stem cutting treatment is carried out; the stubble height of the two stem cuttings is 18 cm.

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