Health-care product capable of relieving fatigue and enhancing immunity and preparation method of health-care product

Abstract

The invention discloses a health-care product capable of relieving fatigue and enhancing immunity and a preparation method of the health-care product. The health-care product is prepared from the following raw materials in parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of cordyceps militaris polypeptide, 1-3 parts of stevioside and 15-20 parts of maltodextrin. The compound enzyme powder is prepared by taking a roselle extract and a schisandra chinensis extract as raw materials and adding lactobacillus rhamnosus NJ551 for fermentation. The health-care product contains a plurality of natural nutritional ingredients, and is prepared by scientific proportioning and fine processing. The components have obvious effects of resisting oxidation, resisting fatigue and enhancing immunity, and can effectively relieve body fatigue, improve body immunity and prevent and treat various diseases.

Description

Technical Field

[0001] The invention belongs to the field of health care products, and specifically relates to a health care product capable of relieving fatigue and enhancing immunity and a preparation method thereof. Background Art

[0002] With the acceleration of modern life and the increase of work pressure, people are more and more prone to fatigue and decreased immunity. Fatigue and low immunity not only affect people's quality of life, but also may lead to the occurrence of various diseases. Therefore, it is of great significance to develop a health product that can relieve fatigue and enhance immunity.

[0003] At present, there are some research reports on health products with the function of relieving fatigue and enhancing immunity. For example, there are studies based on ingredients such as Paecilomyces quinquefolius powder, American ginseng, epimedium and Gynostemma pentaphyllum, which significantly improve immune function and relieve physical fatigue through synergistic effects. In addition, the combination of coenzyme Q10 and grape seed extract has also been shown to have significant anti-fatigue and immune-enhancing effects. This combination achieves its effects by anti-oxidation and regulating the immune system function. In addition, some traditional Chinese medicine ingredients such as astragalus, rhodiola rosea, and ganoderma lucidum are also widely used in health products for anti-fatigue and immune-enhancing. These ingredients usually have the effects of replenishing qi and nourishing yin, nourishing the liver and kidneys, and can achieve the effects of anti-fatigue and enhancing immunity by regulating endocrine and improving microcirculation. The above products vary in efficacy, taste, ease of human absorption, safety, etc., making it difficult for consumers to choose. Summary of the invention

[0004] In view of the deficiencies of the prior art, the purpose of the present invention is to provide a health product with scientific ingredients, significant effects, good taste, easy absorption by the human body and high safety, so as to meet people's needs for relieving fatigue and enhancing immunity.

[0005] The technical solution of the present invention is:

[0006] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of cordyceps militaris polypeptide, 1-3 parts of stevioside and 15-20 parts of maltodextrin.

[0007] Preferably, the health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of Cordyceps militaris polypeptide, 2 parts of stevioside and 15 parts of maltodextrin.

[0008] Preferably, the preparation steps of the composite enzyme powder are:

[0009] (1) soaking dried and mold-free Roselle in clean water, extracting with hot water, filtering, concentrating and drying the filtrate to obtain a Roselle extract;

[0010] (2) washing the schisandra chinensis, drying and then crushing, adding an appropriate amount of water for reflux extraction, filtering, concentrating and drying the filtrate to obtain a schisandra chinensis extract;

[0011] (3) dissolving the roselle extract, the schisandra chinensis extract, and glucose in water, stirring and mixing, and pasteurizing to obtain a fermentation medium;

[0012] (4) inoculating Lactobacillus rhamnosus into a fermentation medium for fermentation, centrifuging the fermentation product, collecting the supernatant and drying it to obtain a composite enzyme powder.

[0013] Furthermore, the mass ratio of the roselle extract, the schisandra chinensis extract, the glucose and the water is 5:5:(3-4):100.

[0014] Furthermore, the viable count of Lactobacillus rhamnosus is 10 9 ~10 10 CFU / mL, the inoculum amount of Lactobacillus rhamnosus was 3% by volume.

[0015] Furthermore, the pasteurization is performed by heating at 65° C. for 20 minutes.

[0016] Preferably, the preparation steps of the Cordyceps militaris polypeptide are:

[0017] (1) extracting selenium-enriched Cordyceps militaris dry powder with acetic acid solution, centrifuging, collecting the supernatant, precipitating with saturated ammonium sulfate, centrifuging, collecting the precipitate, re-dissolving the precipitate with pure water, dialyzing, and drying to obtain selenoprotein dry powder;

[0018] (2) Dissolve the selenoprotein powder in water, adjust the pH to 1.5-3, add pepsin for enzymatic hydrolysis, then adjust the pH to 7.8-8.5, add trypsin for further enzymatic hydrolysis, inactivate the enzyme, filter, separate the filtrate with an ultrafiltration membrane with a molecular weight cutoff of 5 kDa-10 kDa, collect components with a molecular weight between 10 kDa and 5 kDa, dry, and obtain Cordyceps militaris polypeptide.

[0019] Furthermore, the concentration of the acetic acid solution is 5% by volume, and contains 0.1% by volume of β-mercaptoethanol.

[0020] Preferably, the health care product is a capsule or a tablet.

[0021] The present invention also provides a method for preparing the above-mentioned health care product capable of relieving fatigue and enhancing immunity, comprising the following steps: uniformly mixing the compound enzyme powder, Cordyceps militaris polypeptide, stevioside and maltodextrin in the formula amount, filling the mixture into a capsule shell to obtain the health care product in the form of a capsule, or adding an appropriate amount of tablet auxiliary materials, pressing the mixture into tablets to obtain the health care product in the form of a tablet.

[0022] Preferably, the tablet excipients include fillers, disintegrants, glidants and binders.

[0023] Preferably, the filler is microcrystalline cellulose, the disintegrant is cross-linked sodium carboxymethyl cellulose and / or polyvinyl pyrrolidone, the glidant is micro powder silica gel, and the binder is 5% PVP 60% ethanol solution.

[0024] Roselle has a fresh red color and rich nutrients, and is known as the "ruby of the plant world." Roselle is rich in vitamin C, flavonoids, anthocyanins and other antioxidants, which can remove free radicals in the body, reduce oxidative stress, protect cells from damage, and improve the body's endurance and fatigue resistance. Bioactive substances such as flavonoids and anthocyanins have immunomodulatory effects, which can promote the generation and function of immune cells, help the body better cope with the invasion of external pathogens, improve the body's resistance to infection, and thus enhance overall immunity.

[0025] Roselle enzyme is a common health food. Studies have shown that the ingredients contained in Roselle enzyme (such as alcohol, organic acid, fructose, saponin-like compounds, etc.) may irritate the gastrointestinal tract and cause symptoms such as diarrhea. In addition, Roselle itself is cold in nature, and people with weak constitutions are prone to abdominal pain, diarrhea and other discomforts after drinking it. Therefore, although Roselle has certain anti-fatigue and immunity-enhancing effects, health foods made in the form of enzymes are limited to a certain group of people.

[0026] Schisandra chinensis is rich in active ingredients such as quercetin, which can enhance energy metabolism, improve mitochondrial function, and increase muscle oxygen supply, thereby improving athletic performance and reducing fatigue. The schisandra polysaccharide contained in it can enhance the phagocytosis of macrophages, promote lymphocyte transformation, and increase the number and vitality of white blood cells, thereby enhancing the body's defense against diseases. Its active ingredients (such as quercetin) can activate the PI3K-Akt and MAPK signaling pathways, which play an important role in energy metabolism, cell survival and immune regulation. Quercetin further enhances the anti-fatigue and immune regulation effects by increasing the expression levels of related proteins (such as p-PI3K, p-AKT).

[0027] In addition, Schisandra chinensis has the effect of astringing the intestines and stopping diarrhea. Its mechanism of astringing the intestines and stopping diarrhea mainly includes the astringent effect of its sour taste, warming the spleen and kidney, inhibiting the contraction of intestinal smooth muscles, absorbing bacteria and toxins, and invigorating qi and promoting body fluid. When combined with Roselle to make a compound enzyme, it can inhibit the gastrointestinal discomfort of Roselle enzyme. In addition, Schisandra chinensis is warm in nature, which can neutralize the coldness of Roselle and reduce the irritation of the enzyme to the gastrointestinal tract.

[0028] Therefore, the composite enzyme powder prepared with roselle and schisandra chinensis as raw materials not only has excellent anti-fatigue and immunity-enhancing effects, but also significantly reduces gastrointestinal adverse reactions and improves the safety of the enzyme powder.

[0029] In addition, the conventional enzyme preparation process is usually to clean fresh roselle or schisandra, control the water, mix with glucose and water, and seal it for natural fermentation. The enzyme obtained by natural fermentation not only has a low content of active ingredients, but also may produce some substances harmful to the human body during the fermentation process, such as higher alcohols. The present invention optimizes the preparation process of the composite enzyme, firstly uses water extraction to extract the active ingredients of roselle or schisandra, obtains roselle or schisandra extract, and then uses roselle and schisandra extract as raw materials, adds Lactobacillus rhamnosus as a strain for fermentation, and prepares the composite enzyme. The process of the present invention can not only make the active ingredients of roselle and schisandra fully dissolved, but also help to convert the macromolecules in the extract into small molecules, and generate new active ingredients, thereby significantly improving its absorption rate and bioavailability in the human body.

[0030] The Lactobacillus rhamnosus used in the present invention is preferably Lactobacillus rhamnosus NJ551 (deposited in China Center for Type Culture Collection, with a deposit number of CCTCC NO: M2016157), which not only has good gastrointestinal stability, but also has a certain activity of improving immunity. Using this strain as a fermentation strain is not only safe, but also helps to improve the immune activity of the complex enzyme.

[0031] The cordyceps militaris polypeptide of the present invention is prepared by protein extraction, enzymolysis, separation and other steps with selenium-rich cordyceps militaris dry powder as raw material, and the cordyceps militaris polypeptide can increase the generation of ATP (adenosine triphosphate), improve antioxidant capacity, reduce the generation of lactic acid, free radicals and lipid peroxides, thereby achieving the effect of anti-fatigue, and can also stimulate the activation of macrophages, promote T lymphocyte proliferation, and enhance the humoral immunity and cellular immune function of the body. The present invention successively adopts the ultrafiltration membrane of different molecular weight cut-off to separate the enzymolysis product, and obtains the cordyceps militaris polypeptide with molecular weight between 5kDa to 10kDa respectively, the cordyceps militaris polypeptide with molecular weight less than 5kDa, and the cordyceps militaris polypeptide with molecular weight greater than 10kDa. Find that although the polypeptide with molecular weight less than 5kDa is more easily absorbed by the human body, the Cordyceps militaris polypeptide with molecular weight between 5kDa to 10kDa and the composite enzyme powder of the present invention are the best, and the effect is better than the polypeptide with the remaining two molecular weights.

[0032] Furthermore, adding an appropriate amount of stevioside and maltodextrin to health products can help improve the taste and texture of the products and increase the solubility of the products.

[0033] The present invention has the following beneficial effects:

[0034] The health care product of the present invention contains a variety of natural nutrients, which are scientifically proportioned and finely processed. These ingredients have significant anti-oxidation, anti-fatigue and immunity-enhancing effects, can effectively relieve physical fatigue, improve physical immunity, and prevent and treat various diseases.

[0035] The health care product of the present invention not only has a significant health care effect, but also has good taste and safety. The product does not contain any chemical components and additives, has no side effects on the human body, and is highly safe.

[0036] In summary, the health care product of the present invention has broad market prospects and application value, and can provide effective protection for people's health. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 The results of the anti-fatigue test of the health food of the present invention are shown in Table 1. Compared with the blank group, * p<0.05, ** p<0.01.

[0038] Figure 2 The results of the immune enhancement test of the health food of the present invention. A is the effect on the level of immunoglobulin IgG in mice; B is the effect on the level of immunoglobulin IgM in mice; C is the effect on the activity of NK cells in mice. Compared with the blank group, * p<0.05, ** p<0.01, *** p<0.001. DETAILED DESCRIPTION

[0039] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention. The components of the formulations in the following examples are all conventional commercially available products unless otherwise specified.

[0040] Example 1

[0041] 1. Preparation of compound enzyme powder:

[0042] (1) After washing the dried and mold-free roselle, soak it in water for 1 hour, heat it to 80° C. for extraction for 3 hours, filter it, and concentrate the filtrate to dryness to obtain the roselle extract.

[0043] (2) Wash the Schisandra chinensis, dry it, and then crush it. Add an appropriate amount of water, heat it under reflux at 100° C. for 2 h, filter it, and concentrate the filtrate to dryness to obtain the Schisandra chinensis extract.

[0044] (3) Dissolve 50 g of Hibiscus sabdariffa extract, 50 g of Schisandra chinensis extract, and 35 g of glucose in 1000 mL of water, stir and mix, and heat at 65° C. for 20 min for pasteurization to obtain a fermentation medium.

[0045] (4) Lactobacillus rhamnosus NJ551 (viable count of 10 9 ~10 10 CFU / mL) was inoculated into the fermentation medium at a volume fraction of 3% and fermented at 37°C for 72 hours. The fermentation product was centrifuged, and the supernatant was collected and freeze-dried to obtain the composite enzyme powder.

[0046] 2. Preparation of Roselle Enzyme Powder:

[0047] (1) After washing the dried and mold-free roselle, soak it in water for 1 hour, heat it to 80° C. for extraction for 3 hours, filter it, and concentrate the filtrate to dryness to obtain the roselle extract.

[0048] (2) 50 g of roselle extract and 35 g of glucose were dissolved in 1000 mL of water, stirred and mixed, and heated at 65° C. for 20 min for pasteurization to obtain a fermentation medium.

[0049] (3) Lactobacillus rhamnosus NJ551 (viable count of 10 9 ~10 10 CFU / mL) was inoculated into the fermentation medium at a volume fraction of 3% and fermented at 37° C. for 72 h. The fermentation product was centrifuged, and the supernatant was collected and freeze-dried to obtain roselle enzyme powder.

[0050] 3. Preparation of Schisandra chinensis powder:

[0051] (1) Wash the Schisandra chinensis, dry it, and then crush it. Add an appropriate amount of water, heat it under reflux at 100° C. for 2 h, filter it, and concentrate the filtrate to dryness to obtain the Schisandra chinensis extract.

[0052] (2) Dissolve 50 g of Schisandra chinensis extract and 35 g of glucose in 1000 mL of water, stir and mix, and heat at 65° C. for 20 min for pasteurization to obtain a fermentation medium.

[0053] (3) Lactobacillus rhamnosus NJ551 (viable count of 10 9 ~10 10 CFU / mL) was inoculated into the fermentation medium at a volume fraction of 3% and fermented at 37°C for 72 hours. The fermentation product was centrifuged, and the supernatant was collected and freeze-dried to obtain Schisandra chinensis enzyme powder.

[0054] Example 2

[0055] Preparation of Cordyceps militaris polypeptide:

[0056] (1) The selenium-enriched Cordyceps militaris was dried and crushed, and passed through an 80-mesh sieve to obtain selenium-enriched Cordyceps militaris dry powder. The dry powder was added to a 5% acetic acid solution (containing 0.1% β-mercaptoethanol) at a solid-liquid ratio of 1:20 g / mL, stirred at 4°C for 4 hours, centrifuged, and the supernatant was collected. Ammonium sulfate was added to the supernatant to 75% saturation, stirred for 0.5 hours, allowed to stand at 4°C for 12 hours, centrifuged, and the precipitate was collected. The precipitate was redissolved with purified water, dialyzed, and freeze-dried to obtain selenoprotein dry powder.

[0057] (2) Dissolve the selenoprotein powder in water to prepare a 10 mg / mL selenoprotein solution, adjust the pH to 2, add 3% pepsin by weight of the selenoprotein powder, and perform enzymatic hydrolysis at 37°C for 1 hour, then adjust the pH to 8, add 3% trypsin by weight of the selenoprotein powder, and continue enzymatic hydrolysis at 37°C for 1 hour. After the enzyme is inactivated, filter, and separate the filtrate using ultrafiltration membranes with a molecular weight cutoff of 10 kDa and 5 kDa, respectively, to collect components with a molecular weight less than 5 kDa, components with a molecular weight between 5 kDa and 10 kDa, and components with a molecular weight greater than 10 kDa, and freeze-dry to obtain Cordyceps militaris polypeptides of different molecular weights.

[0058] The Cordyceps militaris polypeptide with a molecular weight less than 5 kDa is recorded as CMP1, the Cordyceps militaris polypeptide with a molecular weight between 5 kDa and 10 kDa is recorded as CMP2, and the Cordyceps militaris polypeptide with a molecular weight greater than 10 kDa is recorded as CMP3.

[0059] Example 3

[0060] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 15 parts of compound enzyme powder, 25 parts of cordyceps militaris polypeptide CMP, 1 part of stevioside and 15 parts of maltodextrin.

[0061] Preparation steps: evenly mix the compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside and maltodextrin in the formula amount, and fill them into capsule shells to obtain a health product in capsule dosage form.

[0062] Example 4

[0063] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 25 parts of compound enzyme powder, 10 parts of cordyceps militaris polypeptide CMP2, 3 parts of stevioside and 20 parts of maltodextrin.

[0064] Preparation steps: evenly mix the compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside and maltodextrin in the formula amount, and fill them into capsule shells to obtain a health product in capsule dosage form.

[0065] Example 5

[0066] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of compound enzyme powder, 28 parts of cordyceps militaris polypeptide CMP, 2 parts of stevioside and 15 parts of maltodextrin.

[0067] Preparation steps: evenly mix the compound enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside and maltodextrin in the formula amount, and fill them into capsule shells to obtain a health product in capsule dosage form.

[0068] Example 6

[0069] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of compound enzyme powder, 18 parts of cordyceps militaris polypeptide CMP, 2 parts of stevioside and 15 parts of maltodextrin.

[0070] Preparation steps: Mix the compound enzyme powder, Cordyceps militaris polypeptide CMP1, stevioside and maltodextrin in the formula amount, fill them into capsule shells, and obtain a health product in capsule form.

[0071] Example 7

[0072] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of compound enzyme powder, 38 parts of cordyceps militaris polypeptide CMP, 2 parts of stevioside and 15 parts of maltodextrin.

[0073] Preparation steps: evenly mix the compound enzyme powder, Cordyceps militaris polypeptide CMP3, stevioside and maltodextrin in the formula amount, and fill them into capsule shells to obtain a health product in capsule dosage form.

[0074] Comparative Example 1

[0075] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of roselle enzyme powder, 28 parts of cordyceps militaris polypeptide CMP, 2 parts of stevioside and 15 parts of maltodextrin.

[0076] Preparation steps: Mix the formulated amount of roselle enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside and maltodextrin, and fill them into capsule shells to obtain a health product in capsule form.

[0077] Comparative Example 2

[0078] A health product capable of relieving fatigue and enhancing immunity comprises the following raw materials in parts by weight: 20 parts of schisandra chinensis enzyme powder, 28 parts of cordyceps militaris polypeptide CMP, 2 parts of stevioside and 15 parts of maltodextrin.

[0079] Preparation steps: evenly mix the formulated amounts of Schisandra chinensis enzyme powder, Cordyceps militaris polypeptide CMP2, stevioside and maltodextrin, and fill the mixture into capsule shells to obtain a health product in capsule form.

[0080] Comparative Example 3

[0081] The health care product of Comparative Example 3 differs from the health care product of Example 5 in that the formula does not contain compound enzyme powder, and the other components remain unchanged.

[0082] Comparative Example 4

[0083] The health care product of Comparative Example 4 is different from the health care product of Example 5 in that the formula does not contain Cordyceps militaris polypeptide CMP2, and the other components remain unchanged.

[0084] Application Example 1: Anti-fatigue test

[0085] Kunming mice weighing 18-22g were selected and randomly divided into Example 3-7 group (T3-T7), Comparative Example 1-4 group (D1-D4) and blank control group (CK), with 10 mice in each group. The mice in Example 3-7 group and Comparative Example 1-4 group were gavaged with the contents of the capsule-type health products prepared in Example 3-7 and Comparative Example 1-4, respectively, at a gavage dose of 1g / kg·bw, once a day, for 30 consecutive days. The mice in the blank control group were gavaged with an equal volume of normal saline. 30 minutes after the last gavage of each group of mice, a lead wire of 5% of their body weight was loaded at the base of the tail of the mice, and they were placed in a swimming box to swim until the mice could not swim anymore (for example, they were exhausted or fainted), and the weighted swimming time of each group of mice was measured, and the measurement results were averaged. Results Figure 1 .

[0086] Depend on Figure 1 It can be known that the health products prepared by embodiment 3-5 can increase the weight-bearing exhaustive swimming time of mice, and the effect of the health products prepared by embodiment 5 is the best. It can be known from embodiment 5-7 that compared to the Cordyceps militaris polypeptide (CMP1) with a molecular weight less than 5kDa and the Cordyceps militaris polypeptide (CMP3) with a molecular weight greater than 10kDa, the Cordyceps militaris polypeptide (CMP2) with a molecular weight between 5kDa and 10kDa is the best in compatibility with the composite enzyme powder of the present invention, and the effect is better than the polypeptides of the other two molecular weights. It can be known from comparative example 1-2 that compared to roselle enzyme powder and Schisandra chinensis enzyme powder, the composite enzyme powder of the present invention increases the effect of mice weight-bearing exhaustive swimming time better. It can be known from comparative example 3-4 that the composite enzyme powder of the present invention and Cordyceps militaris polypeptide CMP2 play a synergistic role in increasing the weight-bearing exhaustive swimming time of mice.

[0087] Application Example 2: Immunity Enhancement Test

[0088] Kunming mice weighing 18-22g were selected and randomly divided into Example 3-7 group (T3-T7), Comparative Example 1-4 group (D1-D4) and blank control group (CK), with 10 mice in each group. The mice in Example 3-7 group and Comparative Example 1-4 group were gavaged with the contents of the capsule-type health products prepared in Example 3-7 and Comparative Example 1-4, respectively, at a gavage dose of 1g / kg·bw, once a day, for 30 consecutive days. The mice in the blank control group were given an equal volume of normal saline. On the second day after the gavage, the eyeballs of the mice were removed and blood was collected. After the blood was allowed to stand for 2h, it was centrifuged at 0.4×g for 20min, and then the supernatant was taken and centrifuged at 0.4×g for 5min. The supernatant was taken and the levels of immunoglobulins IgM and IgG were detected using an Elisa kit. Figure 2 The mice were killed by cervical dislocation, and the NK cell activity assay (LDH assay) was performed according to the literature (Yu Ping et al. Study on enhancing the immune function of mice with ginseng glycopeptide complexes of different molecular weights [J]. Food and Fermentation Industries, 2021, 47). The absorbance value of each group was detected by an enzyme label analyzer, and the activity of NK cells was calculated. Figure 2 The results were averaged.

[0089] Depend on Figure 2 It can be seen that the health products prepared by embodiment 3-5 can increase the IgG and IgM levels in mouse serum, enhance the NK cell activity of mice, and the health products prepared by embodiment 5 have the best effect. As shown in embodiment 5-7, the compatibility of Cordyceps militaris polypeptide (CMP2) and the composite enzyme powder of the present invention is the best, and the effect is better than the polypeptides (CMP1 and CMP3) of the remaining two molecular weights. As shown in comparative example 1-2, compared to roselle enzyme powder and Schisandra chinensis enzyme powder, the composite enzyme powder of the present invention increases the IgG and IgM levels in mouse serum, and the effect of enhancing the NK cell activity of mice is better. As shown in comparative example 3-4, the composite enzyme powder of the present invention and Cordyceps militaris polypeptide CMP2 increase the IgG and IgM levels in mouse serum, and enhance the NK cell activity of mice. Play a synergistic role.

[0090] Application Example 3: Gastrointestinal irritation detection

[0091] Kunming mice weighing 18-22g were selected and randomly divided into a blank control group, an embodiment 5 group, and a comparative example 1-2 group, with 6 mice in each group, wherein the mice in the embodiment 5 group and the comparative example 1-2 group were gavaged with the health products prepared in the embodiment 5 and the comparative example 1-2, and the gavage dose was 1g / kg·bw. The mice in the blank control group were gavaged with an equal volume of normal saline. After administration, each mouse was placed in a beaker padded with filter paper, observed for 6h, and the number of diarrhea in the mice was counted. The measurement results were averaged (x-±s), and the results are shown in Table 1.

[0092] Table 1 Gastrointestinal irritation test results

[0093] Group Diarrhea frequency Blank control group 0 Example 5 Group 0 Comparative Example 1 3.47±1.29 Comparative Example 2 0

[0094] As can be seen from the above table, when mice were fed with health products containing compound enzyme powder or Schisandra chinensis enzyme powder (Example 5, Comparative Example 2), the mice did not experience diarrhea, while when mice were fed with health products containing Roselle enzyme powder (Comparative Example 1), the mice experienced diarrhea. This result shows that Schisandra chinensis can effectively inhibit the gastrointestinal irritation of Roselle.

[0095] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A health product that can relieve fatigue and enhance immunity, characterized in that: The preparation comprises the following raw materials in parts by weight: 15-25 parts of compound enzyme powder, 5-10 parts of cordyceps militaris polypeptide, 1-3 parts of stevioside and 15-20 parts of maltodextrin.

2. The health product for relieving fatigue and enhancing immunity according to claim 1, characterized in that: The preparation comprises the following raw materials in parts by weight: 20 parts of compound enzyme powder, 8 parts of cordyceps militaris polypeptide, 2 parts of stevioside and 15 parts of maltodextrin.

3. The health product capable of relieving fatigue and enhancing immunity according to claim 1 or 2, characterized in that: The preparation steps of the composite enzyme powder are as follows: (1) soaking dried and mold-free Roselle in clean water, extracting with hot water, filtering, concentrating and drying the filtrate to obtain a Roselle extract; (2) washing the schisandra chinensis, drying and then crushing, adding an appropriate amount of water for reflux extraction, filtering, concentrating and drying the filtrate to obtain a schisandra chinensis extract; (3) dissolving the roselle extract, the schisandra chinensis extract, and glucose in water, stirring and mixing, and pasteurizing to obtain a fermentation medium; (4) inoculating Lactobacillus rhamnosus into a fermentation medium for fermentation, centrifuging the fermentation product, collecting the supernatant and drying it to obtain a composite enzyme powder.

4. The health product capable of relieving fatigue and enhancing immunity according to claim 3, characterized in that: The mass ratio of the roselle extract, the schisandra chinensis extract, the glucose and the water is 5:5:(3-4):

100.

5. The health product capable of relieving fatigue and enhancing immunity according to claim 3, characterized in that: The viable count of Lactobacillus rhamnosus is 10 9 ~10 10 CFU / mL, the inoculum amount of Lactobacillus rhamnosus was 3% by volume.

6. The health product for relieving fatigue and enhancing immunity according to claim 1, characterized in that: The preparation steps of the Cordyceps militaris polypeptide are as follows: (1) extracting selenium-enriched Cordyceps militaris dry powder with acetic acid solution, centrifuging, collecting the supernatant, precipitating with saturated ammonium sulfate, centrifuging, collecting the precipitate, re-dissolving the precipitate with pure water, dialyzing, and drying to obtain selenoprotein dry powder; (2) Dissolve the selenoprotein powder in water, adjust the pH to 1.5-3, add pepsin for enzymatic hydrolysis, then adjust the pH to 7.8-8.5, add trypsin for further enzymatic hydrolysis, inactivate the enzyme, filter, separate the filtrate with an ultrafiltration membrane with a molecular weight cutoff of 5 kDa-10 kDa, collect components with a molecular weight between 10 kDa and 5 kDa, dry, and obtain Cordyceps militaris polypeptide.

7. The health product for relieving fatigue and enhancing immunity according to claim 6, characterized in that: The concentration of the acetic acid solution is 5% by volume, and contains 0.1% by volume of β-mercaptoethanol.

8. The health product capable of relieving fatigue and enhancing immunity according to claim 1 or 2, characterized in that: The health care product is a capsule or a tablet.

9. A method for preparing the health product capable of relieving fatigue and enhancing immunity as claimed in claim 1 or 2, characterized in that: The method comprises the following steps: mixing the compound enzyme powder, cordyceps militaris polypeptide, stevioside and maltodextrin in a prescribed amount, filling the mixture into a capsule shell to obtain a health product in the form of a capsule, or adding an appropriate amount of tablet auxiliary materials, pressing the mixture into tablets to obtain a health product in the form of a tablet.

10. The preparation method according to claim 9, characterized in that: The tablet auxiliary materials include fillers, disintegrants, glidants and binders.

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