Probiotic composite product for regulating sleep quality and preparation method thereof

Abstract

The invention relates to a probiotic composite product for regulating sleep quality and a preparation method of the probiotic composite product. The probiotic composite product is prepared from the following components: gamma-aminobutyric acid, bifidobacterium breve CCFM1025, lactobacillus acidophilus TYCA06, lactobacillus plantarum LPL28, resistant dextrin, xylooligosaccharide, fructo-oligosaccharide and stachyose. The composition of the three probiotics including the bifidobacterium breve CCFM1025, the lactobacillus acidophilus TYCA06 and the lactobacillus plantarum LPL28 has the effects of high yield of gamma-aminobutyric acid and improvement of sleep quality, the active ingredient gamma-aminobutyric acid can shorten the sleep latency period, and the prebiotics can promote growth and metabolism of the probiotics. The probiotics, the prebiotics and the active ingredients are reasonably matched, so that the product has the effects of regulating sleep and improving the sleep quality.

Description

Technical Field

[0001] The present application relates to the field of food, and in particular to a probiotic compound product for regulating sleep quality and a preparation method thereof. Background Art

[0003] The products currently on the market that regulate sleep disorders include functional supplements (melatonin, theanine) and Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea). The mechanism of action of functional supplements (melatonin, theanine) is to regulate the endocrine and nervous systems by supplementing endogenous hormones or neurotransmitters related to sleep or brain nerves, soothing the mood and relaxing the body and mind, thereby improving sleep quality. However, long-term consumption of these products can cause abnormal hormone metabolism regulation, endocrine disorders and other adverse physical reactions. Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea), the mechanism of action of these products is that the natural ingredients in them can calm the mind, thereby relaxing the body and mind and improving sleep. However, long-term improper consumption of saffron extract can cause endocrine disorders, especially having a greater impact on women's physiology; Chinese jujube kernel is not suitable for some people with allergies and underlying diseases; sleep tea contains caffeine that can stimulate the excitatory nerves, drinking more tea will increase the number of times you get up at night, and herbal tea will stimulate the stomach and intestines.

[0004] There is currently no probiotic product on the market that can regulate sleep quality. Summary of the invention

[0005] In view of the above problems, the present application provides a probiotic compound product suitable for all age groups and for regulating sleep quality, as well as its preparation method and application. The probiotic product can produce γ-aminobutyric acid in the body, thereby improving sleep quality.

[0006] The invention provides a probiotic compound product for regulating sleep quality. The probiotic compound product contains the following components: gamma-aminobutyric acid; Bifidobacterium breve CCFM1025; Lactobacillus acidophilus TYCA06; and Lactobacillus plantarum LPL28.

[0007] Different from the prior art, the above technical scheme provides a probiotic compound product with a new probiotic formula. Bifidobacterium breve CCFM1025, Lactobacillus acidophilus TYCA06, and Lactobacillus plantarum LPL28 can produce γ-aminobutyric acid, which can shorten the sleep latency. The directly added γ-aminobutyric acid can directly provide γ-aminobutyric acid to the body before the probiotics metabolize and produce γ-aminobutyric acid, so that the sleep regulation effect takes effect quickly. γ-Aminobutyric acid (GABA) is an amino acid. In mammals, GABA is produced by glutamate decarboxylase and acts as an inhibitory neurotransmitter in the central nervous system. It can regulate the endocrine and nervous systems. GABA can reduce the number of urinations at night and improve sleep quality.

[0008] Furthermore, the probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus BLa05 and Pediococcus acidilactici CCFM7902.

[0009] Furthermore, the probiotic composite product includes the following components in parts by mass: 80-98 parts by mass of probiotic mixed powder; 0.1-1 part by mass of Bifidobacterium breve CCFM1025; 0.1-0.8 part by mass of Lactobacillus acidophilus TYCA06; 0.1-1 part by mass of Lactobacillus plantarum LPL28; 2-10 parts by mass of γ-aminobutyric acid; the probiotic mixed powder includes the following components in parts by mass: 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharides, 6-10 parts by mass of fructo-oligosaccharides, 6-10 parts by mass of stachyose; 0.5-1.1 parts by mass of Lactobacillus rhamnosus BLa05, and 0.05-0.2 parts by mass of Pediococcus acidilactici CCFM7902.

[0010] The probiotic mixed powder includes four prebiotics: resistant dextrin, xylooligosaccharides, fructooligosaccharides, and stachyose. The above prebiotics provide conditions for the growth of lactic acid bacteria, promote the growth and proliferation of probiotics, and make the effect of probiotic powder better. This technical solution uses the above probiotics, prebiotics, and γ-aminobutyric acid in combination, which can effectively enhance the product's effect of regulating sleep. At the same time, it can promote the proliferation of beneficial intestinal bacteria, inhibit the growth and reproduction of harmful bacteria, selectively regulate intestinal flora, and make it more suitable for a wider range of people. The reasonable combination of probiotics, prebiotics and active ingredients enables the product to regulate sleep and improve sleep quality.

[0011] Furthermore, the probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 40-60 parts by weight of cranberry fruit powder. The addition of erythritol and cranberry fruit powder plays a role in adjusting the taste of the product.

[0012] Furthermore, the content of live bacteria in the probiotic composite product is 1.0×10 11CFU / g or above. At present, the bacterial activity of probiotic products in the domestic market is relatively low, most of which are below 2.0×10 10 CFU / g-6.0×10 10 The probiotics Bifidobacterium breve CCFM1025, Lactobacillus acidophilus TYCA06 and Lactobacillus plantarum LPL28 of the present invention have much higher bacterial activity than other products on the market.

[0013] The present invention also discloses a preparation method of the probiotic composite product, which comprises the following steps:

[0014] Preparation of probiotic mixed powder: adding 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharide, 6-10 parts by mass of fructo-oligosaccharide, 6-10 parts by mass of stachyose, 0.5-1.1 parts by mass of Lactobacillus rhamnosus BLa05, 0.05-0.2 parts by mass of Pediococcus acidilactici CCFM7902, 8-14 parts by mass of erythritol, and 40-60 parts by mass of cranberry fruit powder into a mixing bin, and mixing evenly to obtain the probiotic mixed powder;

[0015] Preparation of probiotic composite powder: adding 0.1-1 mass parts of Bifidobacterium breve CCFM1025, 0.1-0.8 mass parts of Lactobacillus acidophilus TYCA06, 0.1-1 mass parts of Lactobacillus plantarum LPL28, and 2-10 mass parts of γ-aminobutyric acid to 80-98 mass parts of probiotic mixed powder, and mixing to obtain the probiotic composite powder;

[0016] Sealing: canning and sealing the probiotic composite powder to obtain a probiotic composite product.

[0017] Furthermore, the preparation of the probiotic mixed powder and the preparation and sealing of the probiotic composite powder are carried out in a nitrogen atmosphere.

[0018] The inventor found in the study that the outside air has a certain influence on the activity of probiotics during the production process, which will cause the number of live bacteria of probiotics to drop greatly during the shelf life due to problems such as process and packaging. Therefore, the technical solution adopts a vacuum nitrogen filling process to isolate light and air to maintain the stability of the product and the activity of probiotics. In the temporary storage tank for raw materials, a vacuum sealing method is adopted to isolate the raw materials from the external environment; when loading, nitrogen is filled into the silo to place the raw materials in a nitrogen environment; during the canning process, nitrogen is continuously filled into the package, and a film is applied to seal the probiotic powder so that the probiotic powder is in a nitrogen package. By using vacuum nitrogen filling technology, each component is placed in a nitrogen environment throughout the production process, avoiding the problem of product instability and reduced probiotic activity caused by contact with the outside air. In addition, flushing with nitrogen can help control the oxygen and moisture indicators in the inner packaging of the product, better maintain the activity of the product, and slow down the decline in the activity of probiotics during the shelf life.

[0019] Furthermore, in the sealing step, the sealed can is made of food-grade polyethylene.

[0020] The food-grade polyethylene packaging shell is used to effectively block external stimuli such as light and heat, thereby better preserving the activity of probiotics.

[0021] The above-mentioned records related to the invention content are only an overview of the technical solution of the present application. In order to enable ordinary technicians in the field to more clearly understand the technical solution of the present application, and then implement it according to the written contents of the specification, and to make the above-mentioned purpose and other purposes, features and advantages of the present application easier to understand, the specific implementation methods of the present application are explained below. DETAILED DESCRIPTION

[0022] In order to explain the technical content, structural features, achieved objectives and effects of the technical solution in detail, the following is a detailed description in conjunction with specific embodiments.

[0023] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description in conjunction with the specific embodiments listed. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0024] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0025] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0026] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, A and / or B, which means: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in an "or" logical relationship.

[0027] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0028] Without further limitations, in this application, the words "include", "comprises", "has" or other similar expressions used in the sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0029] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0030] Bifidobacterium breve: Bifidobacterium breve is an anaerobic Gram-positive bacillus isolated from the feces of breast-fed infants by French scholar Tissier in 1899. The end of the bacterium often bifurcates, hence the name Bifidobacterium. Bifidobacterium in the human intestine plays an important role in human health. Bifidobacterium is a sign of human health and a beneficial flora that has many benefits for the human body. Bifidobacterium appears in infants 3-4 months after birth. The number of bifidobacteria gradually increases and reaches a peak during early childhood, accounting for about 25% of the total intestinal bacteria. With the increase of age, the number of bifidobacteria decreases year by year, and the number of bifidobacteria decreases to less than 7.9% in the elderly.

[0031] Lactobacillus acidophilus: Lactobacillus acidophilus belongs to the genus Lactobacillus, a Gram-positive bacillus with rounded ends. Its optimum growth temperature is 30-38°C and its optimum pH is 5.5-6.0. It is an anaerobic or facultative anaerobic microorganism. Lactobacillus acidophilus is one of the main microorganisms in the intestinal tract of the host (human and animal). When it exists in a certain number, it can adjust and improve the balance between beneficial and harmful microorganisms in the intestine, thereby improving the health of the host. It is a probiotic that is currently receiving great attention for research and development.

[0032] Lactobacillus plantarum: Lactobacillus plantarum (L. plantarum) is a type of Gram-positive lactobacillus, which is widely found in nature, especially in various fermented foods, such as vegetables, meat, dairy products and wine. At the same time, Lactobacillus plantarum also exists in the human gastrointestinal tract, and is a probiotic flora in the human gastrointestinal tract, which has a great promoting effect on human health.

[0033] Example 1 A probiotic composite product

[0034] Preparation of probiotic mixed powder: 5 parts by mass of resistant dextrin, 8 parts by mass of oligoxylose, 6 parts by mass of oligofructose, 6 parts by mass of stachyose, 0.5 parts by mass of Lactobacillus rhamnosus BLa05, 0.05 parts by mass of Pediococcus acidilactici CCFM7902, 8 parts by mass of erythritol, and 40 parts by mass of cranberry fruit powder are added to the mixing bin, and mixed evenly to obtain the probiotic mixed powder; the mixing process is carried out in a nitrogen environment. Preparation of probiotic composite powder: 80 parts by mass of probiotic mixed powder, 0.1 parts by mass of Bifidobacterium breve CCFM1025, 0.1 parts by mass of Lactobacillus acidophilus TYCA06, 0.1 parts by mass of Lactobacillus plantarum LPL28, and 10 parts by mass of γ-aminobutyric acid are mixed to obtain the probiotic composite powder. The mixing process is carried out in a nitrogen environment.

[0035] Sealing: The probiotic compound powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic compound product.

[0036] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0037] Example 2 A probiotic composite product

[0038] Preparation of probiotic mixed powder: 20 parts by mass of resistant dextrin, 14 parts by mass of xylo-oligosaccharide, 10 parts by mass of fructo-oligosaccharide, 10 parts by mass of stachyose, 1.1 parts by mass of Lactobacillus rhamnosus BLa05, 0.2 parts by mass of Pediococcus acidilactici CCFM7902, 14 parts by mass of erythritol, and 60 parts by mass of cranberry fruit powder are added into a mixing bin, and mixed evenly to obtain the probiotic mixed powder; the mixing process is carried out in a nitrogen environment.

[0039] Preparation of probiotic composite powder: 98 parts by mass of probiotic mixed powder, 1 part by mass of Bifidobacterium breve CCFM1025, 0.8 parts by mass of Lactobacillus acidophilus TYCA06, 1 part by mass of Lactobacillus plantarum LPL28, and 2 parts by mass of γ-aminobutyric acid are mixed to obtain the probiotic composite powder; the mixing process is carried out in a nitrogen environment.

[0040] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0041] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0042] Example 3 A probiotic composite product

[0043] Preparation of probiotic mixed powder: 10 parts by mass of resistant dextrin, 10 parts by mass of oligoxylose, 8 parts by mass of oligofructose, 8 parts by mass of stachyose, 1 part by mass of Lactobacillus rhamnosus BLa05, 0.1 parts by mass of Pediococcus acidilactici CCFM7902, 10 parts by mass of erythritol, and 50 parts by mass of cranberry fruit powder are added to the mixing bin, and mixed evenly to obtain the probiotic mixed powder; the mixing process is carried out in a nitrogen environment. Preparation of probiotic composite powder: 85 parts by mass of probiotic mixed powder, 0.5 parts by mass of Bifidobacterium breve CCFM1025, 0.5 parts by mass of Lactobacillus acidophilus TYCA06, 0.6 parts by mass of Lactobacillus plantarum LPL28, and 5 parts by mass of γ-aminobutyric acid are mixed to obtain the probiotic composite powder; the mixing process is carried out in a nitrogen environment.

[0044] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0045] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0046] Example 4 A probiotic composite product

[0047] Preparation of probiotic mixed powder: 12 parts by mass of resistant dextrin, 12 parts by mass of xylo-oligosaccharide, 10 parts by mass of fructo-oligosaccharide, 8 parts by mass of stachyose, 0.8 parts by mass of Lactobacillus rhamnosus BLa05, 0.09 parts by mass of Pediococcus acidilactici CCFM7902, 11 parts by mass of erythritol, and 50 parts by mass of cranberry fruit powder are added into a mixing bin, and mixed evenly to obtain the probiotic mixed powder; the mixing process is carried out in a nitrogen environment.

[0048] Preparation of probiotic composite powder: 90 parts by mass of probiotic mixed powder, 0.6 parts by mass of Bifidobacterium breve CCFM1025, 0.8 parts by mass of Lactobacillus acidophilus TYCA06, 0.2 parts by mass of Lactobacillus plantarum LPL28, and 9 parts by mass of γ-aminobutyric acid are mixed to obtain the probiotic composite powder; the mixing process is carried out in a nitrogen environment.

[0049] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0050] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0051] Example 5 A probiotic composite product

[0052] Preparation of probiotic mixed powder: 15 parts by mass of resistant dextrin, 13 parts by mass of xylo-oligosaccharide, 8 parts by mass of fructo-oligosaccharide, 7 parts by mass of stachyose, 0.8 parts by mass of Lactobacillus rhamnosus BLa05, 0.15 parts by mass of Pediococcus acidilactici CCFM7902, 12 parts by mass of erythritol, and 55 parts by mass of cranberry fruit powder are added into a mixing bin, and mixed evenly to obtain the probiotic mixed powder; the mixing process is carried out in a nitrogen environment.

[0053] Preparation of probiotic composite powder: 90 parts by mass of probiotic mixed powder, 0.2 parts by mass of Bifidobacterium breve CCFM1025, 0.4 parts by mass of Lactobacillus acidophilus TYCA06, 0.8 parts by mass of Lactobacillus plantarum LPL28, and 7 parts by mass of γ-aminobutyric acid are mixed to obtain the probiotic composite powder; the mixing process is carried out in a nitrogen environment.

[0054] Sealing: The probiotic mixed powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product.

[0055] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0056] This technical solution provides a probiotic compound product with a new probiotic formula. Bifidobacterium breve CCFM1025, Lactobacillus acidophilus TYCA06, and Lactobacillus plantarum LPL28 can produce γ-aminobutyric acid, which can shorten the sleep latency period. The directly added γ-aminobutyric acid can directly provide γ-aminobutyric acid to the body before the probiotics metabolize and produce γ-aminobutyric acid, so that the effect of regulating sleep quality takes effect quickly. γ-Aminobutyric acid (GABA) is an amino acid. In mammals, GABA is produced by glutamate decarboxylase and acts as an inhibitory neurotransmitter in the central nervous system. It can regulate the endocrine and nervous systems. GABA can reduce the number of urinations at night and improve sleep quality.

[0057] Finally, it should be noted that although the above embodiments have been described in the text of the specification of this application, this does not limit the scope of patent protection of this application. All technical solutions generated by replacing or modifying equivalent structures or equivalent processes based on the essential concept of this application using the contents recorded in the text of the specification of this application, as well as directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, are included in the scope of patent protection of this application.

Claims

1. A probiotic compound product for regulating sleep quality, characterized in that: The probiotic compound product contains the following components: gamma-aminobutyric acid, Bifidobacterium breve CCFM1025, Lactobacillus acidophilus TYCA06 and Lactobacillus plantarum LPL28.

2. The probiotic composite product according to claim 1, characterized in that: The probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus BLa05 and Pediococcus acidilactici CCFM7902.

3. The probiotic composite product according to claim 1, characterized in that: The probiotic composite product includes the following components in parts by mass: 80-98 parts by mass of probiotic mixed powder; 0.1-1 part by mass of Bifidobacterium breve CCFM1025; 0.1-0.8 part by mass of Lactobacillus acidophilus TYCA06; 0.1-1 part by mass of Lactobacillus plantarum LPL28; 2-10 parts by mass of γ-aminobutyric acid; the probiotic mixed powder includes the following components in parts by mass: 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharides, 6-10 parts by mass of fructo-oligosaccharides, 6-10 parts by mass of stachyose; 0.5-1.1 parts by mass of Lactobacillus rhamnosus BLa05, and 0.05-0.2 parts by mass of Pediococcus acidilactici CCFM7902.

4. The probiotic composite product according to claim 3, characterized in that: The probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 40-60 parts by weight of cranberry fruit powder.

5. The probiotic composite product according to any one of claims 1 to 4, characterized in that: The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

6. The method for preparing the probiotic composite product according to claim 4, characterized in that: The preparation method comprises the following steps: Preparation of probiotic mixed powder: adding 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharide, 6-10 parts by mass of fructo-oligosaccharide, 6-10 parts by mass of stachyose, 0.5-1.1 parts by mass of Lactobacillus rhamnosus BLa05, 0.05-0.2 parts by mass of Pediococcus acidilactici CCFM7902, 8-14 parts by mass of erythritol, and 40-60 parts by mass of cranberry fruit powder into a mixing bin, and mixing evenly to obtain the probiotic mixed powder; Preparation of probiotic composite powder: adding 0.1-1 mass parts of Bifidobacterium breve CCFM1025, 0.1-0.8 mass parts of Lactobacillus acidophilus TYCA06, 0.1-1 mass parts of Lactobacillus plantarum LPL28, and 2-10 mass parts of γ-aminobutyric acid to 80-98 mass parts of the probiotic mixed powder, and mixing to obtain the probiotic composite powder; Sealing: canning and sealing the probiotic composite powder to obtain a probiotic composite product.

7. The preparation method according to claim 6, characterized in that: The preparation of the probiotic mixed powder and the preparation and sealing steps of the probiotic composite powder are carried out in a nitrogen atmosphere.

8. The preparation method according to claim 6, characterized in that: In the sealing step, the sealed can is made of food-grade polyethylene.