Probiotic composite product for improving emotion and preparation method thereof

Abstract

The invention relates to a probiotic composite product for improving emotion and a preparation method of the probiotic composite product. The probiotic composite product comprises the following probiotic components: lactobacillus helveticus R0052, lactobacillus plantarum R1012 and bifidobacterium longum R0175. The combination of the three probiotics has a synergistic effect, and can reduce the ratio of kynurenine / tryptophan in a human body and reduce metabolic kynurenine. The probiotic composition can also reduce cortisol and adrenal gland levels, relieve depression and improve mood.

Description

Technical Field

[0001] The present application relates to the field of food, and in particular to a probiotic compound product for improving mood and a preparation method thereof. Background Art

[0002] With the accelerated pace of life, work and life pressure, mood swings, and irregular diet and rest have become the norm for modern people, and various health problems have seriously interfered with our lives. Among them, sleep disorders plague people of different ages and are also a precursor to many chronic diseases. Sleep disorders refer to abnormalities in sleep quality and quantity, as well as a series of clinical symptoms that occur during sleep. Long-term sleep disorders can affect personal physiological functions and mental health, not only reducing the quality of life, but also inducing mental illnesses and cardiovascular and cerebrovascular diseases.

[0003] The products currently on the market that regulate sleep disorders include functional supplements (melatonin, theanine) and Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea). The mechanism of action of functional supplements (melatonin, theanine) is to regulate the endocrine and nervous systems by supplementing endogenous hormones or neurotransmitters related to sleep or brain nerves, soothing the mood and relaxing the body and mind, thereby improving sleep quality. However, long-term consumption of these products can cause abnormal hormone metabolism regulation, endocrine disorders and other adverse physical reactions. Chinese medicinal ingredients / plant extracts (saffron extract, Chinese jujube kernel and sleep tea), the mechanism of action of these products is that the natural ingredients in them can calm the mind, thereby relaxing the body and mind and improving sleep. However, long-term improper consumption of saffron extract can cause endocrine disorders, especially having a greater impact on women's physiology; Chinese jujube kernel is not suitable for some people with allergies and underlying diseases; sleep tea contains caffeine that can stimulate the excitatory nerves, drinking more tea will increase the number of times you get up at night, and herbal tea will stimulate the stomach and intestines.

[0004] A large number of studies have shown that sleep disorders can be effectively alleviated through emotional regulation, stress relief and improvement of the sleep process. However, there is currently no probiotic product on the market that can improve sleep through emotional regulation. Summary of the invention

[0005] In view of the above problems, the present application provides a probiotic compound product suitable for use in all age groups and for improving mood. The probiotic product can reduce cortisol and adrenal levels, relieve depression, and improve mood.

[0006] The invention provides a probiotic compound product for improving mood. The probiotic compound product contains the following probiotic components: Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175.

[0007] Different from the prior art, the above technical scheme provides a probiotic compound product with a new probiotic formula. The three probiotic combinations of Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175 have a synergistic effect, which can reduce the ratio of kynurenine / tryptophan in the human body and reduce the metabolic kynurenine. Kynurenine competes with tryptophan to cross the blood-brain barrier. The more kynurenine produced in the intestine, the less tryptophan is used in the brain to make 5-hydroxytryptamine. 5-hydroxytryptamine plays an important role in sleep regulation, pain sensitivity, appetite and other functions. Intestinal microorganisms can break down the food eaten and affect the synthesis and secretion of 5-hydroxytryptamine in the intestine. The 5-hydroxytryptamine released in the intestine can have an important effect on brain function. When stimulated, they will generate long-distance vagus nerve signals to the emotion regulation network in the brain, sending signals to the brain in this way. In addition, the study found that the probiotic combination can reduce cortisol and adrenal levels, relieve depression and improve mood.

[0008] Furthermore, the probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus and Pediococcus acidilactici.

[0009] Furthermore, the probiotic composite product includes the following components in parts by mass: 80-95 parts by mass of probiotic mixed powder; 0.5-10 parts by mass of Lactobacillus helveticus R0052, 0.5-10 parts by mass of Lactobacillus plantarum R1012, and 0.5-10 parts by mass of Bifidobacterium longum R0175; the probiotic mixed powder includes the following components in parts by mass: 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharides, 0.5-1.1 parts by mass of Lactobacillus rhamnosus, 0.05-0.2 parts by mass of Pediococcus acidilactici, 6-10 parts by mass of fructo-oligosaccharides, and 6-10 parts by mass of stachyose.

[0010] The probiotic mixed powder includes four prebiotics: resistant dextrin, xylooligosaccharide, fructooligosaccharide, and stachyose. The above prebiotics provide conditions for the growth of lactic acid bacteria, promote the growth and proliferation of probiotics, and make the probiotic powder more effective. This technical solution uses the above probiotics and prebiotics in combination, which can effectively soothe emotions and enhance the emotional management effect of the product. At the same time, it can promote the proliferation of beneficial intestinal bacteria, inhibit the growth and reproduction of harmful bacteria, and selectively regulate the intestinal flora, making it more suitable for a wider range of people.

[0011] Furthermore, the probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 40-60 parts by weight of cranberry fruit powder. The addition of erythritol and cranberry fruit powder plays a role in adjusting the taste of the product.

[0012] Furthermore, the mass ratio of Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 in the probiotic composite product is 1:1:1.

[0013] Furthermore, the content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g or above. At present, the bacterial activity of probiotic products in the domestic market is relatively low, most of which are below 2.0×10 10 CFU / g-6.0×10 10 The probiotics Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175 of the present invention are all preferred strains, and their bacterial activity is much higher than other products on the market.

[0014] The present invention also discloses a preparation method of the probiotic composite product, which comprises the following steps:

[0015] Preparation of probiotic mixed powder: Lactobacillus rhamnosus and Pediococcus acidilactici are mixed in proportion, and resistant dextrin, xylooligosaccharide, fructooligosaccharide, stachyose, erythritol and cranberry fruit powder are added in proportion, and the mixture is evenly mixed to obtain the probiotic mixed powder;

[0016] Preparation of probiotic composite powder: Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 are mixed evenly in proportion, and then mixed evenly with the probiotic mixed powder in proportion to obtain probiotic composite powder;

[0017] Sealing: canning and sealing the probiotic mixed powder to obtain a probiotic composite product.

[0018] Furthermore, the steps of preparing the probiotic mixed powder, preparing the probiotic composite powder and sealing are carried out in a nitrogen atmosphere.

[0019] The inventor found in the study that the outside air has a certain influence on the activity of probiotics during the production process, which will cause the number of live bacteria of probiotics to drop greatly during the shelf life due to problems such as process and packaging. Therefore, the technical solution adopts a vacuum nitrogen filling process to isolate light and air to maintain the stability of the product and the activity of probiotics. In the temporary storage tank for raw materials, a vacuum sealing method is adopted to isolate the raw materials from the external environment; when loading, nitrogen is filled into the silo to place the raw materials in a nitrogen environment; during the canning process, nitrogen is continuously filled into the package, and a film is applied to seal the probiotic powder so that the probiotic powder is in a nitrogen package. By using vacuum nitrogen filling technology, each component is placed in a nitrogen environment throughout the production process, avoiding the problem of product instability and reduced probiotic activity caused by contact with the outside air. In addition, filling with nitrogen can help control the oxygen and moisture indicators in the inner packaging of the product, better maintain the activity of the product, and slow down the decline in the activity of probiotics during the shelf life.

[0020] Furthermore, in the sealing step, the sealed can is made of food-grade polyethylene.

[0021] The food-grade polyethylene packaging shell is used to effectively block external stimuli such as light and heat, thereby better preserving the activity of probiotics.

[0022] The above-mentioned records related to the invention content are only an overview of the technical solution of the present application. In order to enable ordinary technicians in the field to more clearly understand the technical solution of the present application, and then implement it according to the written contents of the specification, and to make the above-mentioned purpose and other purposes, features and advantages of the present application easier to understand, the specific implementation methods of the present application are explained below. DETAILED DESCRIPTION

[0023] In order to explain the technical content, structural features, achieved objectives and effects of the technical solution in detail, the following is a detailed description in conjunction with specific embodiments.

[0024] In order to explain in detail the possible application scenarios, technical principles, specific schemes that can be implemented, and the purposes and effects that can be achieved, the following is a detailed description in conjunction with the specific embodiments listed. The embodiments described herein are only used to more clearly illustrate the technical solutions of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0025] Reference to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The term "embodiment" appearing in various places in the specification does not necessarily refer to the same embodiment, nor does it particularly limit its independence or association with other embodiments. In principle, in the present application, as long as there is no technical contradiction or conflict, the various technical features mentioned in the embodiments can be combined in any way to form a corresponding implementable technical solution.

[0026] Unless otherwise defined, the technical terms used in this document have the same meanings as those generally understood by those skilled in the art to which this application belongs; the use of relevant terms in this document is only for describing specific embodiments and is not intended to limit this application.

[0027] In the description of this application, the term "and / or" is an expression used to describe the logical relationship between objects, indicating that three relationships may exist, for example, A and / or B, which means: A exists, B exists, and A and B exist at the same time. In addition, the character " / " in this article generally indicates that the objects before and after are in an "or" logical relationship.

[0028] In the present application, terms such as “first” and “second” are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship of quantity, priority or sequence between these entities or operations.

[0029] Without further limitations, in this application, the words "include", "comprises", "has" or other similar expressions used in sentences are intended to cover non-exclusive inclusion. These expressions do not exclude the presence of additional elements in the process, method or product including the elements, so that the process, method or product including a series of elements may include not only those limited elements, but also other elements not explicitly listed, or also include elements inherent to such process, method or product.

[0030] Similar to the understanding in the Examination Guidelines, in this application, expressions such as "greater than", "less than", "exceed" and the like are understood to exclude the number itself; expressions such as "above", "below", "within" and the like are understood to include the number itself. In addition, in the description of the embodiments of this application, "multiple" means more than two (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups", "multiple times", etc., unless otherwise clearly and specifically limited.

[0031] Lactobacillus helveticus: Lactobacillus helveticus is a Gram-positive lactobacillus with an optimal growth temperature of 42°C. It is usually white or light gray and opaque, and the diameter of the colony on the agar plate is 2-3 mm or less. It produces DL-lactic acid by homotypic fermentation, and produces acid slowly or not in mannose and fructose. Lactobacillus helveticus has recognized safety and probiotic properties and is widely used in dairy products, such as producing a large amount of lactic acid in milk, being able to rapidly cleave cheese, and expressing complex proteolytic enzyme sequences.

[0032] Lactobacillus plantarum: The optimal growth temperature of Lactobacillus plantarum (L. plantarum) is 30-35℃. It is anaerobic or facultative anaerobic. The strain is straight or curved rod-shaped, single, sometimes in pairs or chains. The optimal pH is about 6.5. It belongs to homofermentative lactic acid bacteria. As a probiotic flora of the human gastrointestinal tract, Lactobacillus plantarum has multiple functions such as maintaining the balance of intestinal flora, improving the body's immunity and promoting the absorption of nutrients. Bifidobacterium longum: Bifidobacterium is a Gram-positive, non-motile, rod-shaped cell, and one end is sometimes forked. Strictly anaerobic bacteria. As beneficial intestinal microorganisms, Bifidobacterium plays an important role in maintaining the health of the body. Twelve of the 32 known species of Bifidobacterium are derived from humans. Bifidobacterium longum is found in the intestinal microbiota of infants and adults. The most common species of Bifidobacterium can colonize the human intestine. The U.S. Food and Drug Administration (FDA) and the European Food Safety Authority (EFSA) have recognized that Bifidobacterium longum is safe, and Chinese laws and regulations have also approved the use of Bifidobacterium longum in food and health foods. Bifidobacterium longum plays an important role in human health, with physiological functions such as preventing and treating constipation, inhibiting intestinal pathogens, regulating intestinal balance, lowering cholesterol, promoting digestion and absorption of nutrients, delaying aging, and enhancing the body's immune activity.

[0033] Embodiment 1 A kind of probiotic compound product:

[0034] Preparation of probiotic mixed powder: 0.5 mass parts of Lactobacillus rhamnosus and 0.05 mass parts of Pediococcus acidilactici are mixed, and then 5 mass parts of resistant dextrin, 8 mass parts of xylo-oligosaccharides, 6 mass parts of fructo-oligosaccharides, 6 mass parts of stachyose, 8 mass parts of erythritol and 40 mass parts of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after uniform mixing, the probiotic mixed powder is obtained.

[0035] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing kettle, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 85:5:5:5.

[0036] Sealing: The probiotic compound powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic compound product.

[0037] The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

[0038] Example 2 A probiotic composite product

[0039] Preparation of probiotic mixed powder: 1.1 parts by mass of Lactobacillus rhamnosus and 0.2 parts by mass of Pediococcus acidilactici are mixed, and then 20 parts by mass of resistant dextrin, 14 parts by mass of xylo-oligosaccharide, 10 parts by mass of fructo-oligosaccharide, 10 parts by mass of stachyose, 14 parts by mass of erythritol and 60 parts by mass of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after uniform mixing, the probiotic mixed powder is obtained.

[0040] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing bin, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 95:10:10:10.

[0041] Sealing: The probiotic composite powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product. The probiotic composite product has a live bacteria content of 1.0×10 11 CFU / g and above.

[0042] Example 3 A probiotic composite product

[0043] Preparation of probiotic mixed powder: 1 part by mass of Lactobacillus rhamnosus and 0.1 part by mass of Pediococcus acidilactici are mixed, and then 10 parts by mass of resistant dextrin, 10 parts by mass of xylo-oligosaccharide, 8 parts by mass of fructo-oligosaccharide, 8 parts by mass of stachyose, 10 parts by mass of erythritol and 50 parts by mass of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after uniform mixing, the probiotic mixed powder is obtained.

[0044] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing bin, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 90:0.5:10:10.

[0045] Sealing: The probiotic composite powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product. The probiotic composite product has a live bacteria content of 1.0×10 11 CFU / g and above.

[0046] Example 4 A probiotic composite product

[0047] Preparation of probiotic mixed powder: 0.8 mass parts of Lactobacillus rhamnosus and 0.12 mass parts of Pediococcus acidilactici are mixed, and then 15 mass parts of resistant dextrin, 10 mass parts of xylo-oligosaccharides, 8 mass parts of fructo-oligosaccharides, 7 mass parts of stachyose, 12 mass parts of erythritol and 50 mass parts of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after uniform mixing, the probiotic mixed powder is obtained.

[0048] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing bin, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 80:10:0.5:10.

[0049] Sealing: The probiotic composite powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product. The probiotic composite product has a live bacteria content of 1.0×10 11 CFU / g and above.

[0050] Example 5 A probiotic composite product

[0051] Preparation of probiotic mixed powder: 1.1 parts by mass of Lactobacillus rhamnosus and 0.2 parts by mass of Pediococcus acidilactici are mixed, and then 5 parts by mass of resistant dextrin, 14 parts by mass of xylo-oligosaccharide, 10 parts by mass of fructo-oligosaccharide, 10 parts by mass of stachyose, 14 parts by mass of erythritol and 60 parts by mass of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after uniform mixing, the probiotic mixed powder is obtained.

[0052] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing bin, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 90:10:10:0.5.

[0053] Sealing: The probiotic composite powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product. The probiotic composite product has a live bacteria content of 1.0×10 11 CFU / g and above.

[0054] Example 6 A probiotic composite product

[0055] Preparation of probiotic mixed powder: After mixing 0.7 parts by mass of Lactobacillus rhamnosus and 0.1 parts by mass of Pediococcus acidilactici, 10 parts by mass of resistant dextrin, 8 parts by mass of xylo-oligosaccharide, 6 parts by mass of fructo-oligosaccharide, 10 parts by mass of stachyose, 12 parts by mass of erythritol, and 60 parts by mass of cranberry fruit powder are added, and the mixing process is carried out in a nitrogen environment; after mixing evenly, the probiotic mixed powder is obtained.

[0056] Preparation of probiotic composite powder: Add Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 into the mixing bin, mix evenly to obtain probiotic composite powder; wherein the ratio of probiotic mixed powder, Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 is 82:6:8:7.

[0057] Sealing: The probiotic composite powder is canned and sealed in a food-grade polyethylene packaging can in a nitrogen atmosphere to obtain a probiotic composite product. The probiotic composite product has a live bacteria content of 1.0×10 11 CFU / g and above.

[0058] In this technical solution, the three probiotic combinations of Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175 have a synergistic effect, which can reduce the ratio of kynurenine / tryptophan in the human body and reduce the metabolic kynurenine. Kynurenine competes with tryptophan to cross the blood-brain barrier. The more kynurenine produced in the intestine, the less tryptophan is used in the brain to make 5-hydroxytryptamine. 5-hydroxytryptamine plays an important role in sleep regulation, pain sensitivity, appetite and other functions. Intestinal microorganisms can decompose the food eaten and affect the synthesis and secretion of 5-hydroxytryptamine in the intestine. The 5-hydroxytryptamine released in the intestine can have an important effect on brain function. When stimulated, they will generate long-distance vagus nerve signals to the emotional regulation network in the brain, sending signals to the brain in this way. This probiotic combination can reduce cortisol and adrenal levels, relieve depression and improve mood.

[0059] Finally, it should be noted that although the above embodiments have been described in the text of the specification of this application, this does not limit the scope of patent protection of this application. All technical solutions generated by replacing or modifying equivalent structures or equivalent processes based on the essential concept of this application using the contents recorded in the text of the specification of this application, as well as directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, are included in the scope of patent protection of this application.

Claims

1. A probiotic compound product for improving mood, characterized in that: The probiotic compound product contains the following probiotic components: Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175.

2. The probiotic composite product according to claim 1, characterized in that: The probiotic compound product also includes the following probiotic components: Lactobacillus rhamnosus and Pediococcus acidilactici.

3. The probiotic composite product according to claim 1, characterized in that: The probiotic composite product comprises the following components in parts by mass: 80-95 parts by mass of probiotic mixed powder; 0.5-10 parts by mass of Lactobacillus helveticus R0052, 0.5-10 parts by mass of Lactobacillus plantarum R1012, and 0.5-10 parts by mass of Bifidobacterium longum R0175; the probiotic mixed powder comprises the following components in parts by mass: 5-20 parts by mass of resistant dextrin, 8-14 parts by mass of xylo-oligosaccharide, 0.5-1.1 parts by mass of Lactobacillus rhamnosus, 0.05-0.2 parts by mass of Pediococcus acidilactici, 6-10 parts by mass of fructo-oligosaccharide, and 6-10 parts by mass of stachyose.

4. The probiotic composite product according to claim 3, characterized in that: The probiotic mixed powder also includes: 8-14 parts by weight of erythritol and 40-60 parts by weight of cranberry fruit powder.

5. The probiotic composite product according to claim 1, characterized in that: The mass ratio of Lactobacillus helveticus R0052, Lactobacillus plantarum R1012 and Bifidobacterium longum R0175 in the probiotic composite product is 1:1:

1.

6. The probiotic composite product according to any one of claims 1 to 5, characterized in that: The content of live bacteria in the probiotic composite product is 1.0×10 11 CFU / g and above.

7. The method for preparing the probiotic composite product according to claim 4, characterized in that: The preparation method comprises the following steps: Preparation of probiotic mixed powder: Lactobacillus rhamnosus and Pediococcus acidilactici are mixed in proportion, and resistant dextrin, xylooligosaccharide, fructooligosaccharide, stachyose, erythritol and cranberry fruit powder are added in proportion, and the mixture is evenly mixed to obtain the probiotic mixed powder; Preparation of probiotic composite powder: Lactobacillus helveticus R0052, Lactobacillus plantarum R1012, and Bifidobacterium longum R0175 are mixed evenly in proportion, and then mixed evenly with the probiotic mixed powder in proportion to obtain the probiotic composite powder; Sealing: canning and sealing the probiotic composite powder to obtain a probiotic composite product.

8. The preparation method according to claim 7, characterized in that: The steps of preparing the probiotic mixed powder, preparing the probiotic composite powder and sealing are carried out in a nitrogen atmosphere.

9. The preparation method according to claim 7, characterized in that: In the sealing step, the sealed can is made of food-grade polyethylene.