Construction method of rat abdominal aorta vascular balloon injury model

Abstract

The invention belongs to the technical field of animal model construction, and provides a method for constructing a rat abdominal aorta vascular balloon injury model, which comprises the following steps: S1, selecting a male SD rat as an experimental animal; s2, the male SD rat is fixed to an operating table in a supine position, and the abdomen of the male SD rat is depilated and disinfected; s3, skin and muscular layers are cut open at the middle-lower part of the abdomen of the male SD rat, the male SD rat enters the abdominal cavity, the iliac general artery is separated out, and threading is conducted on the lower portion of the iliac general artery for use; s4, supporting the common iliac artery by using microscopic tweezers to block blood flow; s5, a sharp knife is used for ripping the blood vessel wall in the length direction of the iliac general artery, and the coronary PTA balloon is fed; according to the method, the common iliac artery approach is selected to replace the common carotid artery approach, the pipe diameter of the common iliac artery is larger than that of the common carotid artery, the difficulty of model construction is lowered, the common iliac artery enters the aorta abdominalis more visually and visually through the common iliac artery approach, the operation difficulty is further lowered, and model construction is easier.

Description

Technical Field

[0001] The invention belongs to the technical field of animal model construction, in particular to a method for constructing a rat abdominal aorta vascular balloon injury model. Background Art

[0002] Clinically, vascular restenosis may occur after coronary stent implantation in cardiology, vascular bypass surgery, and balloon dilatation for arteriovenous fistula stenosis in nephrology. The mechanism is vascular intimal hyperplasia. In order to conduct in-depth research on intimal hyperplasia, it is necessary to establish an animal model.

[0003] Currently, the abdominal aorta injury model of rats is mainly made through the common carotid artery approach. However, the common carotid artery is small, which makes it difficult to construct. The 2Fforgaty balloon used in the existing technology has been discontinued. At the same time, the path from the common carotid artery to the abdominal aorta is too long, and the balloon entry path and arrival position are not visible, which increases the difficulty of operation.

[0004] To this end, those skilled in the art have proposed a method for constructing a rat abdominal aorta vascular balloon injury model to solve the problems raised by the background technology. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a method for constructing a rat abdominal aorta vascular balloon injury model to solve the problems that the common carotid artery is small in the prior art, which makes the construction difficult, and the 2Fforgaty balloon used in the prior art has been discontinued. At the same time, the path of the common carotid artery entering the abdominal aorta is too long, and the balloon entry path and arrival position are not visible, which increases the difficulty of operation.

[0006] A method for constructing a rat abdominal aorta balloon injury model, comprising:

[0007] S1: Male SD rats were selected as experimental animals;

[0008] S2: The male SD rats were fixed on the operating table in a supine position, and the abdomen of the male SD rats was depilated and disinfected;

[0009] S3: Cut the skin and muscle layer in the middle and lower abdomen of male SD rats, enter the abdominal cavity, separate the common iliac artery, and thread a suture underneath it for later use;

[0010] S4: Use micro forceps to prop up the common iliac artery to block blood flow;

[0011] S5: Use a sharp knife to cut the vessel wall along the length of the common iliac artery and insert the coronary PTA balloon;

[0012] S6: Deliver the coronary PTA balloon 3.0 cm in, connect the inflation pressure pump to expand the coronary PTA balloon, keep the coronary PTA balloon in an open state and withdraw it, repeat the delivery and withdrawal action 3 times to damage the vascular endothelium;

[0013] S7: After withdrawing the coronary PTA balloon, heparinized saline is injected into the common iliac artery to prevent thrombosis;

[0014] S8: Ligate both sides of the incision of the common iliac artery with silk thread to prevent bleeding, and suture the muscle layer and skin in sequence;

[0015] S9: 21 days after surgery, the chest and abdomen of male SD rats were opened after anesthesia, and the thoracic aorta was found, ligated and cut off;

[0016] S10: Inject 4% formaldehyde into the thoracic aorta to fix it and prevent the intima from falling off;

[0017] S11: Free and cut the abdominal aorta and store it in fixative.

[0018] Preferably, the body weight of the male SD rats is in the range of 250-350 g.

[0019] Preferably, the male SD rats are fasted for 6 hours before surgery.

[0020] Preferably, the male SD rat is fixed in a supine position in front of an operating table and anesthetized by isoflurane inhalation.

[0021] Preferably, when the micro forceps are used to prop up the common iliac artery, a vascular clamp is used to assist in blocking blood flow.

[0022] Preferably, the diameter of the coronary PTA balloon is 2.5 mm, and the expansion pressure of the coronary PTA balloon is 6 atmospheres.

[0023] Preferably, the concentration of the heparin saline is 50 I U / 100 g.

[0024] Preferably, the concentration of the formaldehyde fixative is 4%.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. The present invention selects the common iliac artery approach to replace the common carotid artery approach. Since the common iliac artery is thicker than the common carotid artery, the difficulty of model construction is reduced. In addition, entering the abdominal aorta through the common iliac artery approach is more intuitive and visible, which further reduces the difficulty of operation and makes model construction easier.

[0027] 2. The present invention replaces the 2Fforgaty balloon with a coronary PTA balloon, which has lower cost, is easier to obtain, and is more conducive to model construction.

[0028] 3. The present invention uses formaldehyde fixative to keep the thoracic aorta and the abdominal aorta stable for a long time, thereby completing the construction of the model. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a flowchart of a method for constructing a rat abdominal aorta balloon injury model;

[0030] Figure 2 This is a schematic diagram of a normal male SD rat with undamaged blood vessels;

[0031] Figure 3 Schematic diagram of vascular balloon injury in normal male SD rats;

[0032] Figure 4 Schematic diagram of vascular balloon injury in diabetic male SD rats. DETAILED DESCRIPTION

[0033] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0034] Embodiment 1

[0035] As attached Figure 1 To Attachment Figure 4 As shown, this embodiment provides a method for constructing a rat abdominal aorta balloon injury model, comprising:

[0036] S1: Male SD rats were selected as experimental animals. Male SD rats have strong adaptability and the experimental results are highly repeatable and representative.

[0037] S2: The male SD rats were fixed in a supine position on the operating table to ensure the fixation of the head, abdomen and limbs of the male SD rats to facilitate subsequent surgical operations. The abdomen of the male SD rats was dehaired and disinfected with iodine to reduce the risk of postoperative infection.

[0038] S3: Cut the skin and muscle layer in the middle and lower abdomen of male SD rats, enter the abdominal cavity, separate the common iliac artery, and thread a line underneath it for later use to block blood flow in subsequent operations;

[0039] S4: Use micro forceps to gently prop up the common iliac artery to block blood flow and prevent bleeding during surgery;

[0040] S5: Use a sharp knife to cut the vessel wall along the length of the common iliac artery and insert the coronary PTA balloon into the common iliac artery;

[0041] S6: Deliver the coronary PTA balloon 3.0 cm in, connect the inflation pressure pump to expand the coronary PTA balloon, pay attention to the expansion pressure of the coronary PTA balloon to avoid explosion caused by exceeding the rated pressure, keep the coronary PTA balloon in an open state and slowly withdraw it, repeat the delivery and withdrawal action 3 times to damage the vascular endothelium;

[0042] S7: After withdrawing the coronary PTA balloon, heparinized saline is injected into the common iliac artery to prevent thrombosis;

[0043] S8: Ligate both sides of the common iliac artery incision with silk thread to prevent bleeding, and suture the muscle layer and skin in sequence to ensure that the wound is tightly closed;

[0044] S9: 21 days after surgery, the chest and abdomen of male SD rats were opened after anesthesia, and the thoracic aorta was found, ligated and cut off;

[0045] S10: inject formaldehyde fixative into the thoracic aorta, and then inject formaldehyde fixative into the abdominal aorta through the thoracic aorta to prevent the intima from falling off during the cutting of the abdominal aorta;

[0046] S11: Free and cut the abdominal aorta and store it in formaldehyde fixative.

[0047] From the above, we can see that by choosing the common iliac artery approach instead of the common carotid artery approach, the difficulty of model construction is reduced because the diameter of the common iliac artery is thicker than that of the common carotid artery. In addition, the model is constructed by entering the abdominal aorta through the common iliac artery approach, which has a shorter path and is more intuitive and visual. The operation is simple and reduces the difficulty and time cost of the operation. The coronary PTA balloon is in sufficient supply and low price in the market. Compared with the discontinued 2Fforgaty balloon, the experimental cost is significantly reduced.

[0048] As attached Figure 2 To Attachment Figure 4 As shown, the control experiment can provide a normal male SD rat model and a diabetic male SD rat model for the basic research on intimal hyperplasia. The model shows that the normal vascular intima is distributed in a single layer on the inner side of the smooth muscle cells; after the vascular balloon injury of the normal male SD rat, the intima thickens asymmetrically; after the vascular balloon injury of the diabetic male SD rat, the hyperplastic intima is easy to fall off from the vascular wall, and the intimal hyperplasia of the diabetic male SD rat is more obvious. By making the diabetic male SD rat model, it is confirmed that the pathological male SD rat is also suitable for making a model using this method, which is convenient for judging the tolerance of male SD rats.

[0049] Embodiment 2

[0050] As attached Figure 1As shown, this embodiment is basically the same as the previous embodiment, except that the weight range of male SD rats is 250-350g. Male SD rats in this weight range are usually in early adulthood, with stable physiological functions, mature vascular structure and function, and are suitable for research.

[0051] Specifically, male SD rats were fasted for 6 hours before surgery to reduce the risk of anesthesia.

[0052] Furthermore, male SD rats were fixed in supine position in front of the operating table and anesthetized with isoflurane inhalation, and the corneal reflex, respiratory rate, and heart rate of the male SD rats were checked regularly to ensure that the depth of anesthesia was appropriate.

[0053] Embodiment 3

[0054] As attached Figure 1 As shown, this embodiment is basically the same as the previous embodiment, except that when micro forceps are used to prop up the common iliac artery, a vascular clamp is used to assist in blocking the blood flow. When blocking the blood flow, ensure that the vascular clamp is accurately positioned to avoid accidental clamping of surrounding tissues.

[0055] Specifically, the diameter of the coronary PTA balloon is 2.5 mm, which is suitable for the inner diameter of the common iliac artery of male SD rats, and the expansion pressure of the coronary PTA balloon is 6 atmospheres.

[0056] Furthermore, the concentration of heparin saline is 50 I U / 100 g, and this concentration of heparin saline is used to prevent thrombosis while avoiding excessive stimulation to blood vessels.

[0057] Furthermore, the concentration of the formaldehyde fixative is 4% to ensure the fixation effect on the tissue.

[0058] The standard parts used in the present invention can all be purchased from the market, and the special-shaped parts can be customized according to the description and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0059] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0060] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0061] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0062] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0063] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0064] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for constructing a rat abdominal aorta balloon injury model, characterized in that: include, S1: Male SD rats were selected as experimental animals; S2: The male SD rats were fixed on the operating table in a supine position, and the abdomen of the male SD rats was depilated and disinfected; S3: Cut the skin and muscle layer in the middle and lower abdomen of male SD rats, enter the abdominal cavity, separate the common iliac artery, and thread a suture underneath it for later use; S4: Use micro forceps to prop up the common iliac artery to block blood flow; S5: Use a sharp knife to cut the vessel wall along the length of the common iliac artery and insert the coronary PTA balloon; S6: Deliver the coronary PTA balloon 3.0 cm in, connect the inflation pressure pump to expand the coronary PTA balloon, keep the coronary PTA balloon in an open state and withdraw it, repeat the delivery and withdrawal action 3 times to damage the vascular endothelium; S7: After withdrawing the coronary PTA balloon, heparinized saline is injected into the common iliac artery to prevent thrombosis; S8: Ligate both sides of the incision of the common iliac artery with silk thread to prevent bleeding, and suture the muscle layer and skin in sequence; S9: 21 days after surgery, the chest and abdomen of male SD rats were opened after anesthesia, and the thoracic aorta was found, ligated and cut off; S10: Inject formaldehyde fixative into the thoracic aorta to prevent intimal shedding; S11: Free and cut the abdominal aorta and store it in formaldehyde fixative.

2. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 1, characterized in that: The body weight of the male SD rats ranged from 250 to 350 g.

3. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 2, characterized in that: The male SD rats were fasted for 6 hours before surgery.

4. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 3, characterized in that: The male SD rats were fixed in supine position in front of the operating table and anesthetized by isoflurane inhalation.

5. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 4, characterized in that: When the common iliac artery is propped up with the micro forceps, a vascular clamp is used to assist in blocking the blood flow.

6. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 5, characterized in that: The diameter of the coronary PTA balloon is 2.5 mm, and the expansion pressure of the coronary PTA balloon is 6 atmospheres.

7. The method for constructing a rat abdominal aorta balloon injury model according to claim 6, characterized in that: The concentration of the heparin saline is 50 IU / 100 g.

8. The method for constructing a rat abdominal aorta balloon injury model as claimed in claim 7, characterized in that: The concentration of the formaldehyde fixative is 4%.

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