Composition with moisturizing effect as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a moisturizing effect and a preparation method and application thereof. The composition comprises the following components in percentage by weight: 0.3%-0.8% of a plant fermentation product, 1.2%-2.0% of a plant enzymolysis product, 15%-20% of a humectant, 1.6%-2.5% of a chondrus crispus extract, 0.5%-1.1% of a Chinese herbaceous peony extract, 2.6%-3.9% of a rosemary extract and the balance of water. The composition is suitable for people with allergic reaction caused by face dryness, has a good moisturizing effect, has a long-acting moisturizing effect, also has repairing and anti-aging effects, is mild and non-irritant, and can be used for preparing face moisturizing products.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition with moisturizing effect and a preparation method and application thereof. Background Art

[0002] When exposed to dry conditions for a long time, human skin tends to lose moisture and become dry. Skin in this dry state is more fragile and prone to cracking, which damages the skin's natural protective layer and makes the skin more sensitive to various stimuli in the environment. In addition, such conditions may make it easier for external harmful substances such as dust and bacteria to penetrate the skin barrier, causing skin inflammation or allergic reactions. In order to prevent skin problems caused by dryness, including allergies and other discomforts, it is important to keep the skin fully hydrated.

[0003] For sensitive skin with damaged skin barrier, it is often difficult to effectively relieve and repair skin conditions by simply replenishing external moisture. This is because a damaged skin barrier will affect the skin's water retention function. Even if the skin's moisture content is temporarily increased by external means, due to the decrease in the skin's own water retention ability, this water may be lost quickly, resulting in a rapid decrease in the moisture content of the stratum corneum. This not only fails to solve the problem of dryness, but may further damage the skin barrier due to continued water loss, exacerbating the dryness and sensitivity of the skin.

[0004] Therefore, in this case, it is particularly important to improve the skin's own moisturizing ability. This means not only replenishing moisture for the skin from the outside, but also taking measures to promote moisture retention inside the skin, such as using skin care products containing ingredients such as ceramide and hyaluronic acid, which can help repair the skin barrier and enhance the skin's water-locking function. Although there are many products on the market containing ingredients such as ceramide and hyaluronic acid, the quality varies, especially for people with allergic reactions caused by dry face, they are often more irritating.

[0005] Therefore, there is an urgent need for a composition with moisturizing effect and a preparation method thereof. Summary of the invention

[0006] The purpose of the present invention is to provide a composition with moisturizing effect and a preparation method and application thereof.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A composition with moisturizing effect comprises the following components in percentage by weight: 0.3% to 0.8% of plant fermentation product, 1.2% to 2.0% of plant enzymolysis product, 15% to 20% of moisturizer, 1.6% to 2.5% of Chondrus crispus extract, 0.5% to 1.1% of peony extract, 2.6% to 3.9% of rosemary extract, and the balance is water.

[0009] Furthermore, the method for preparing the plant fermentation product comprises the following steps:

[0010] (1) mixing hibiscus (HIBISCUSROSA-SINENSIS) leaves, Mirabilis jala (MIRABILIS JALAPA), and Atractylodes chinensis (ATRACTYLODES CHINENSIS), and crushing them to a particle size of less than 100 meshes to obtain a fermentation raw material;

[0011] (2) mixing fermentation raw materials and water in a weight ratio of 1:(12-14) to obtain a fermentation liquid; adding mixed bacteria to the fermentation liquid to perform anaerobic fermentation at a fermentation temperature of 36-40° C. for 24-30 hours, and sterilizing after the fermentation to obtain a fermentation product;

[0012] (3) The fermentation product is filtered using a 0.22 μm filter membrane, and the filtrate is subjected to reduced pressure rotary evaporation and dried to obtain a plant fermentation product.

[0013] Furthermore, the weight ratio of hibiscus leaves, Mirabilis jalapa and Atractylodes lancea is 1:(0.3-0.5):(1.4-1.6).

[0014] Furthermore, the mixed bacteria include Bifidobacterium lactis, Lactobacillus casei and Lactobacillus helveticus.

[0015] Furthermore, the amount of Bifidobacterium lactis in the fermentation broth is 10 8 -10 9 CFU / mL; the amount of Lactobacillus casei in the fermentation broth was 10 8 -10 9 CFU / mL; the amount of Lactobacillus helveticus in the fermentation broth was 10 9 -10 10 CFU / mL.

[0016] In the northern region, many people suffer from allergic reactions due to dry faces in winter, mainly because in the northern region, due to low temperature, low air humidity, and the use of indoor heating, the air becomes drier. Such environmental conditions can easily lead to dry facial skin, which may then cause allergic reactions. Moisturizing products on the market can easily lead to aggravated allergic reactions for this group of people, and the moisturizing effect is not ideal. The present invention can improve the moisturizing effect of the composition by selecting a variety of plant extracts and moisturizing ingredients, and adding plant fermentation products. Because through the synergistic effect of multiple components, the active ingredients can complement each other, make up for the shortcomings of a single ingredient, and can synergistically promote absorption, thereby achieving a better moisturizing effect. However, the long-lasting moisturizing effect of the composition is not ideal.

[0017] People with dry face that cause allergic reactions have problems with moisturizing. After the skin barrier is damaged, the repair ability decreases, and symptoms such as dryness, desquamation, and erythema are prone to recurrence. The present invention can play a role in repairing the skin barrier by adding a specific content of Chondrus crispus extract, peony extract, and rosemary extract, and synergizing with the components in the system. Chondrus crispus extract mainly contains seaweed polysaccharides, amino acids, minerals, etc., has an antioxidant effect, can neutralize free radicals, and protect the skin from oxidative stress damage. Peony extract can soothe the skin, and rosemary extract can promote the regeneration and repair of skin cells and enhance the self-repair ability of the skin. After Chondrus crispus extract, peony extract, and rosemary extract are added in a specific ratio, their respective bioactive ingredients play a synergistic role in moisturizing, anti-inflammatory, anti-oxidation, soothing, and promoting cell regeneration, and can enhance the joint repair and enhancement of skin barrier function.

[0018] Furthermore, the method for preparing the plant enzymatic hydrolysate comprises the following steps:

[0019] (1) mixing the root of Bupleurum chinense, Citrus medica sarcodactylis and Calendula officinalis, and crushing the mixture to a size of less than 100 meshes to obtain an enzymatic hydrolysis raw material;

[0020] (2) mixing the enzymatic hydrolysis raw material and water in a weight ratio of 1:(12-14) to obtain an enzymatic hydrolysis solution; adding cellulase to the enzymatic hydrolysis solution, enzymolyzing at 30-35° C. for 5-8 hours, inactivating the enzyme, adding pectinase, enzymolyzing at 30-35° C. for 3-5 hours, inactivating the enzyme, and finally adding neutral protease, enzymolyzing at 30-35° C. for 4-6 hours, inactivating the enzyme, and vacuum drying to a moisture content of 2-5wt% to obtain an enzymatic hydrolysis product;

[0021] (3) mixing the enzymatic hydrolysis product with an ethanol aqueous solution having a volume concentration of 65-70%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 220-240 W and 40-45° C. for 2-3 h to obtain an extract;

[0022] (4) The extract was filtered using a 0.22 μm filter membrane, and the filtrate was evaporated under reduced pressure and dried to obtain a plant enzymatic hydrolysate.

[0023] Furthermore, the weight ratio of Bupleurum root, Citron and Calendula officinalis flower is (0.2-0.4):1:(1.4-1.6).

[0024] Furthermore, the amount of cellulase in the hydrolysate is 100U / mL-120U / mL; the amount of pectinase in the hydrolysate is 70U / mL-90U / mL; and the amount of neutral protease in the hydrolysate is 130U / mL-150U / mL.

[0025] Dry skin lacks elasticity, is prone to fine lines and wrinkles, and accelerates the skin aging process. The present invention can not only promote the moisturizing effect of plant fermentation products by adding plant enzymatic hydrolysis products, but also improve the anti-aging effect of the composition after adding plant enzymatic hydrolysis products. Bupleurum saponin and flavonoids have strong antioxidant capacity, can neutralize free radicals, reduce the damage of oxidative stress to skin cells, and delay skin aging; flavonoids and vitamin C in bergamot have strong antioxidant capacity, can protect the skin from free radical damage; flavonoids and carotenoids in marigold have antioxidant effects, can protect skin cells from oxidative stress damage; using specific enzymes for enzymatic hydrolysis can release more bioactive ingredients, enhance its antioxidant, anti-inflammatory, and promote collagen synthesis effects.

[0026] Furthermore, the moisturizing agent comprises 1,3-butylene glycol, allantoin and glycerin in a weight ratio of 1:(1.2-1.5):(0.6-0.8).

[0027] The present invention uses a moisturizer composed of 1,3-butanediol, allantoin and glycerin in a specific ratio to compound with a plant fermentation product to form a chemical moisturizer. Through the synergistic effect of the chemical moisturizer and the plant moisturizer, the long-lasting moisturizing effect of the composition can be improved.

[0028] The invention provides a preparation method of the composition with moisturizing effect, comprising the following steps: adding a moisturizer into a dry reaction container, controlling the temperature at 45-50°C and the speed at 100-120 r / min, slowly adding water, controlling the same temperature and speed to continue stirring and mixing evenly, cooling to 30-35°C, adding the remaining components, stirring evenly, cooling to room temperature, and obtaining the composition with moisturizing effect.

[0029] The invention provides application of the composition with moisturizing effect in preparing cosmetics.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0031] 1. The present invention can improve the moisturizing effect of the composition for people with allergic reactions caused by dry face by selecting a variety of plant extract ingredients and moisturizing ingredients and adding plant fermentation products.

[0032] 2. The present invention can repair the skin barrier by adding a specific amount of Chondrus crispus extract, peony extract, and rosemary extract, which work synergistically with the components in the system.

[0033] 3. The present invention can not only promote the moisturizing effect of the plant fermentation product by adding the plant enzymatic hydrolysate, but also improve the anti-aging effect of the composition after adding the plant enzymatic hydrolysate.

[0034] 4. The present invention can improve the long-term moisturizing effect of the composition by compounding a moisturizer consisting of 1,3-butylene glycol, allantoin and glycerin in a specific ratio with a plant fermentation product. DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] The raw materials used in the following examples of the present invention are all commercially available products:

[0037] Chondrus crispus extract was purchased from Shaanxi Pinhong Biotechnology Co., Ltd. Chondrus crispus extract.

[0038] Peony extract was purchased from Shaanxi Pinhong Biotechnology Co., Ltd. with a specification of 95%.

[0039] Rosemary extract was purchased from Shaanxi Pinhong Biotechnology Co., Ltd. with a specification of 95%.

[0040] Bifidobacterium lactis, with an activity of 10 billion CFU / g. Collection number: SHBCC D24675. Deposited in Shanghai Microbiological Collection Center.

[0041] Lactobacillus casei, with an activity of 10 billion CFU / g. Collection number: SHBCC D24737. Deposited in Shanghai Microbiological Collection Center.

[0042] Lactobacillus helveticus, with an activity of 20 billion CFU / g. Deposit number: CCTCCAB 2010205; deposited in China Center for Type Culture Collection.

[0043] Bifidobacterium longum, with an activity of 10 billion CFU / g. Collection number: CCTCC HB 20082718. Deposited in China Center for Type Culture Collection.

[0044] Lactobacillus bulgaricus, bacterial activity 20 billion CFU / g. Collection number: CCTCC CB 20082295; deposited in China Center for Type Culture Collection.

[0045] Lactobacillus acidophilus, with an activity of 20 billion CFU / g. Collection number: CCTCCAB 2010208. Deposited in China Center for Type Culture Collection.

[0046] The enzymes used in the present invention were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0047] Allantoin, Yunnan Xili Biotechnology Co., Ltd., CAS: 97-59-6.

[0048] Example 1

[0049] The present embodiment provides a composition with a moisturizing effect, characterized in that it comprises the following components in weight percentage: 0.5% of plant fermentation product, 1.6% of plant enzymatic hydrolysis product, 18% of moisturizer, 2.0% of Chondrus crispus extract, 0.7% of peony extract, 3.1% of rosemary extract, and the balance is water; the moisturizer comprises 1,3-butylene glycol, allantoin and glycerol in a weight ratio of 1:1.4:0.7.

[0050] The method for preparing the plant fermentation product comprises the following steps:

[0051] (1) mixing hibiscus leaves, Mirabilis jalapa, and Atractylodes lancea in a weight ratio of 1:0.4:1.5, and crushing the mixture to a particle size of less than 100 meshes to obtain a fermentation raw material;

[0052] (2) mixing fermentation raw materials and water in a weight ratio of 1:13 to obtain a fermentation liquid; adding mixed bacteria to the fermentation liquid to perform anaerobic fermentation at a fermentation temperature of 38° C. for a fermentation time of 26 h, and sterilizing after the fermentation is completed to obtain a fermentation product;

[0053] (3) The fermentation product is filtered using a 0.22 μm filter membrane, and the filtrate is subjected to reduced pressure rotary evaporation and dried to obtain a plant fermentation product.

[0054] The mixed bacteria include Bifidobacterium lactis, Lactobacillus casei and Lactobacillus helveticus. The amount of Bifidobacterium lactis in the fermentation broth is 10 8 CFU / mL; the amount of Lactobacillus casei in the fermentation broth was 10 9 CFU / mL; the amount of Lactobacillus helveticus in the fermentation broth was 10 9 CFU / mL.

[0055] The method for preparing the plant enzymatic hydrolysate comprises the following steps:

[0056] (1) mixing bupleurum root, bergamot and marigold flower in a weight ratio of 0.3:1:1.5, and crushing to less than 100 mesh to obtain an enzymatic hydrolysis raw material;

[0057] (2) Mixing the enzymatic hydrolysis raw materials and water in a weight ratio of 1:13 to obtain an enzymatic hydrolysis solution; adding cellulase to the enzymatic hydrolysis solution, performing enzymatic hydrolysis at 32°C for 6 hours, inactivating the enzyme, adding pectinase, performing enzymatic hydrolysis at 32°C for 4 hours, inactivating the enzyme, and finally adding neutral protease, performing enzymatic hydrolysis at 32°C for 5 hours, inactivating the enzyme, and vacuum drying to a moisture content of 4wt% to obtain an enzymatic hydrolysis product; the amount of cellulase used in the enzymatic hydrolysis solution is 110U / mL; the amount of pectinase used in the enzymatic hydrolysis solution is 80U / mL; and the amount of neutral protease used in the enzymatic hydrolysis solution is 140U / mL.

[0058] (3) mixing the enzymatic hydrolysis product with an ethanol aqueous solution having a volume concentration of 68%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 230 W and 42° C. for 2.5 h to obtain an extract;

[0059] (4) The extract was filtered using a 0.22 μm filter membrane, and the filtrate was evaporated under reduced pressure and dried to obtain a plant enzymatic hydrolysate.

[0060] The preparation method of the composition with moisturizing effect comprises the following steps: adding a moisturizer into a dry reaction container, controlling the temperature at 48°C and the speed at 110 r / min, slowly adding water, controlling the same temperature and speed to continue stirring and mixing evenly, cooling to 32°C, adding the remaining components, stirring evenly, and cooling to room temperature to obtain a composition with moisturizing effect.

[0061] Example 2

[0062] The present embodiment provides a composition with a moisturizing effect, characterized in that it comprises the following components in weight percentage: 0.4% of plant fermentation product, 2.0% of plant enzymatic hydrolysis product, 15% of moisturizer, 2.5% of Chondrus crispus extract, 1.1% of peony extract, 3.9% of rosemary extract, and the balance is water; the moisturizer comprises 1,3-butanediol, allantoin and glycerol in a weight ratio of 1:1.2:0.6.

[0063] The method for preparing the plant fermentation product comprises the following steps:

[0064] (1) mixing hibiscus leaves, Mirabilis jalapa, and Atractylodes lancea in a weight ratio of 1:0.3:1.4, and crushing the mixture to a particle size of less than 100 meshes to obtain a fermentation raw material;

[0065] (2) mixing fermentation raw materials and water in a weight ratio of 1:12 to obtain a fermentation liquid; adding mixed bacteria to the fermentation liquid to perform anaerobic fermentation at a fermentation temperature of 36° C. for 30 hours; and sterilizing after the fermentation to obtain a fermentation product;

[0066] (3) The fermentation product is filtered using a 0.22 μm filter membrane, and the filtrate is subjected to reduced pressure rotary evaporation and dried to obtain a plant fermentation product.

[0067] The mixed bacteria include Bifidobacterium lactis, Lactobacillus casei and Lactobacillus helveticus. The amount of Bifidobacterium lactis in the fermentation broth is 10 9 CFU / mL; the amount of Lactobacillus casei in the fermentation broth was 10 8 CFU / mL; the amount of Lactobacillus helveticus in the fermentation broth was 10 9 CFU / mL.

[0068] The method for preparing the plant enzymatic hydrolysate comprises the following steps:

[0069] (1) mixing bupleurum root, bergamot and marigold flower in a weight ratio of 0.2:1:1.6, and crushing to less than 100 mesh to obtain an enzymatic hydrolysis raw material;

[0070] (2) Mixing enzymatic hydrolysis raw materials and water in a weight ratio of 1:14 to obtain an enzymatic hydrolysis solution; adding cellulase to the enzymatic hydrolysis solution, performing enzymatic hydrolysis at 30°C for 8 hours, inactivating the enzyme, adding pectinase, performing enzymatic hydrolysis at 30°C for 5 hours, inactivating the enzyme, and finally adding neutral protease, performing enzymatic hydrolysis at 30°C for 6 hours, inactivating the enzyme, and vacuum drying to a moisture content of 5wt% to obtain an enzymatic hydrolysis product; the amount of cellulase in the enzymatic hydrolysis solution is 100U / mL; the amount of pectinase in the enzymatic hydrolysis solution is 90U / mL; and the amount of neutral protease in the enzymatic hydrolysis solution is 130U / mL.

[0071] (3) mixing the enzymatic hydrolysis product with an ethanol aqueous solution having a volume concentration of 70%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 22 W and 45° C. for 2 h to obtain an extract;

[0072] (4) The extract was filtered using a 0.22 μm filter membrane, and the filtrate was evaporated under reduced pressure and dried to obtain a plant enzymatic hydrolysate.

[0073] The preparation method of the composition with moisturizing effect comprises the following steps: adding a moisturizer into a dry reaction container, controlling the temperature at 50°C and the speed at 100 r / min, slowly adding water, controlling the same temperature and speed to continue stirring and mixing evenly, cooling to 35°C, adding the remaining components, stirring evenly, cooling to room temperature, and obtaining the composition with moisturizing effect.

[0074] Comparative Example 1

[0075] The difference between this comparative example and Example 1 is that hibiscus leaves, Mirabilis jalapa and Atractylodes lancea in a weight ratio of 1:1:1 are mixed and crushed to a particle size of less than 100 meshes to obtain a fermentation raw material.

[0076] Comparative Example 2

[0077] The difference between this comparative example and Example 1 is that the mixed bacteria include Bifidobacterium longum, Lactobacillus bulgaricus and Lactobacillus acidophilus. The amount of Bifidobacterium longum in the fermentation broth is 10 8CFU / mL; the dosage of Lactobacillus bulgaricus in the fermentation broth was 10 9 CFU / mL; the amount of Lactobacillus acidophilus in the fermentation broth is 10 9 CFU / mL.

[0078] Comparative Example 3

[0079] The difference between this comparative example and Example 1 is that: 2.0% of Chondrus crispus extract, 0.7% of peony extract, and 3.1% of rosemary extract are replaced by: 2.0% of tea extract, 0.7% of Astragalus (ASTRAGALUS COMPLANATUS) extract, and 3.1% of daisy (BELLIS PERENNIS) flower extract. The tea extract was purchased from Nanjing Tongying Biotechnology Co., Ltd., with a tea polyphenol content of 98% by weight. The Astragalus extract was purchased from Shaanxi Huinengda Biotechnology Co., Ltd., with a specification of 10:1. The daisy (BELLIS PERENNIS) flower extract was purchased from Shaanxi Ciyuan Biotechnology Co., Ltd., with a specification of 10:1.

[0080] Comparative Example 4

[0081] The difference between this comparative example and Example 1 is that: 2.0% of Chondrus crispus extract, 0.7% of Paeonia lactiflora extract, and 3.1% of Rosemary extract are replaced by: 3.1% of Chondrus crispus extract, 2.0% of Paeonia lactiflora extract, and 0.7% of Rosemary extract.

[0082] Comparative Example 5

[0083] The difference between this comparative example and Example 1 is that the root of bupleurum, citron and marigold flower in a weight ratio of 1:1:1 are mixed and crushed to less than 100 meshes to obtain an enzymatic hydrolysis raw material.

[0084] Comparative Example 6

[0085] The difference between this comparative example and Example 1 is that the enzymolysis raw materials and water in a weight ratio of 1:13 are mixed to obtain an enzymolysis solution; bromelain is added to the enzymolysis solution, enzymolysis is carried out at 32° C. for 6 hours, the enzyme is inactivated, ficin is added, enzymolysis is carried out at 32° C. for 4 hours, the enzyme is inactivated, and finally β-glucanase is added, enzymolysis is carried out at 32° C. for 5 hours, the enzyme is inactivated, and vacuum drying is carried out to a moisture content of 4wt% to obtain an enzymolysis product.

[0086] Comparative Example 7

[0087] The difference between this comparative example and Example 1 is that the moisturizing agent comprises 1,3-butylene glycol, allantoin and glycerin in a weight ratio of 1:1:1.

[0088] Performance Testing

[0089] The compositions with moisturizing effect prepared in Examples 1-2 and Comparative Examples 1-7 were mixed with water in a ratio of 1:2 by volume, stirred evenly, and used as samples to perform the following performance tests.

[0090] 1. Test population: Volunteers aged between 20 and 50 years old were selected. All volunteers were diagnosed with allergic reaction symptoms caused by dry face in dermatology department and randomly divided into 9 groups, 30 people in each group;

[0091] Test method: First clean the skin of the test area with physiological saline and wait for 5 minutes to dry; select a qualified patch tester, take 0.5mL of sample into the patch tester in a closed patch test, and apply special tape to the back of the subject, and ask the subject how it feels; bathing is prohibited during the test; the subject keeps the patch area dry within 48 hours, avoids intense exercise, scratching the patch area, and long-term sunlight exposure, etc. After 48 hours, remove the tester and mark it. After 30 minutes, wait for the indentation to disappear and make a judgment under sufficient light. Record the results according to the skin reaction grade standard in the "Hygiene Standards for Skin Care Products". The skin adverse reaction grade standard is shown in Table 1 below. If there is any discomfort during the process, stop the test immediately. The results are shown in Table 2.

[0092] Table 1 Grading of skin adverse reactions

[0093]

[0094] Table 2 Human skin patch test results

[0095]

[0096]

[0097] As shown in Table 2, the composition prepared by the present invention is safe and non-irritating to patients with allergic reaction symptoms caused by dry face.

[0098] 2. Moisture test

[0099] According to QB / T 4256-2011 Cosmetic Moisturizing Efficacy Evaluation Guide, 20 volunteers were selected for each sample. All volunteers were diagnosed with allergic reaction symptoms caused by dry face in dermatology. The samples were quantitatively applied to the circled area of ​​the forearm. The instrument MOISTUREMETERSC MSC1212 (DELFIN) was used to measure the hydration content of the skin stratum corneum at 0h (initial value of unused samples), 1h, and 8h after using the samples. The results are shown in Table 3.

[0100] Table 3 Moisturizing test results (au)

[0101] 0h 1h 8h Example 1 22.15 53.16 35.76 Example 2 22.20 51.73 33.98 Comparative Example 1 22.58 41.40 26.87 Comparative Example 2 22.47 42.82 25.24 Comparative Example 3 22.81 41.03 26.81 Comparative Example 4 22.05 43.28 25.19 Comparative Example 5 22.19 43.54 24.50 Comparative Example 6 22.63 44.96 26.78 Comparative Example 7 22.28 47.13 24.54

[0102] From the results in Table 2, it can be seen that the hydration content of the stratum corneum of the skin of Examples 1-2 of the present invention is greater than 50 a.u. after 1 hour of using the sample, the plant raw material ratio and the type of mixed bacteria used in the preparation of the plant fermentation product in Comparative Examples 1-2 are changed, and the hydration content of the stratum corneum of the skin decreases. After 8 hours of use, the hydration content of the stratum corneum of the skin of Comparative Example 7 is almost restored to the initial value, indicating that changing the composition of the moisturizer will reduce the long-term moisturizing effect. After 8 hours of testing, Examples 1-2 of the present invention still maintain a high hydration content of the stratum corneum of the skin, and have a better and longer-lasting moisturizing effect.

[0103] 3. Skin barrier repair test

[0104] The level of transepidermal water loss (TEWL value) is an important indicator for evaluating the strength of skin barrier function. TEWL value is measured by a transepidermal water loss tester.

[0105] Volunteers aged between 20 and 50 were selected. All of them were diagnosed with allergic reactions caused by dry face in dermatology. 18 volunteers, 2 in each group, were marked on the inner side of the forearms of both hands of the subjects with a size of 3×3 cm. 2 Test area, 6 areas were marked on the same arm, with an interval of 1cm. There were 3 areas for test samples and blank controls, both randomly distributed on the left and right arms. The test area and the control area were measured using a capacitive skin tester. Each area was measured 15 times in parallel, first measuring the blank value of each test area, and then measuring the blank value of each test area at 0.5g / 9cm 2 The amount is evenly applied in the test area. The skin moisture content of the test area and the blank control area is measured 8 hours and 12 hours after application (measured at this time during verification). No products (cosmetics or topical medicines) can be used on the test area 2-3 days before. The test environment temperature is controlled at 25±1℃, and the relative humidity is 40±5%. Before the test, the arms of the subjects are wiped with pure water at about 36℃, and the subjects are asked to sit quietly in the test environment for 30 minutes before the test. According to the experimental design, the TEWL values ​​of each time period are measured separately, and the reduction in skin moisture loss at each time point is calculated. The greater the reduction in skin moisture loss, the better the effect of skin barrier repair. The results are shown in Table 4.

[0106] Calculation formula: reduction in skin water loss = [(T0-T1) / T0] × 100%, where T1 is the TEWL of the test group at each time point, and T0 is the TEWL of the blank control group at each time point.

[0107] Table 4 Skin barrier repair test results

[0108] 8h 12h Example 1 23.1 18.6 Example 2 22.4 17.3 Comparative Example 1 15.7 13.8 Comparative Example 2 16.4 12.7 Comparative Example 3 14.2 11.3 Comparative Example 4 15.9 12.5 Comparative Example 5 16.1 13.2 Comparative Example 6 17.3 15.1 Comparative Example 7 18.0 14.6

[0109] As shown in Table 4, the reduction in skin moisture loss in Examples 1-2 at 8 and 12 hours is large, indicating that the compositions of Examples 1 and 2 have less moisture loss during the long-term moisturizing process, indicating that the composition provided by the present invention can form a stable and long-lasting water-locking barrier on the surface of the skin. However, in Comparative Examples 3 and 4, the ratio of Chondrus crispus extract, Paeonia lactiflora extract, and Rosemary extract is changed or replaced with other types of extracts, and the repair effect of the composition is reduced.

[0110] 4. Anti-aging test

[0111] Take 5 mg of DPPH (1,1-diphenyl 2-picrylhydrazyl (free radical)) and dissolve it in 100 ml of solvent (anhydrous ethanol), dissolve it by ultrasonic for 5 minutes, and mix it thoroughly. Take 5 ml of the DPPH solution and test the absorbance A value at 519 nm. The A value is optimally between 1.2-1.3, and the DPPH standard solution is prepared. The composition is prepared into 0.5 mg / ml sample solution to be tested with anhydrous ethanol, take 5 ml of the sample solution and add it to 5 ml of the DPPH standard solution and mix it evenly, put it in a dark room at room temperature to react for 0.5 h, take 7.5 ml of the sample solution and add it to 10 ml of anhydrous ethanol solution and mix it evenly, and test the absorbance value at 519 nm.

[0112] DPPH free radical scavenging rate (%) = 1-(A2 / A1) × 100%; A1: absorbance value of a mixed solution consisting of a DPPH solution and anhydrous ethanol; A2: absorbance value of a mixed solution consisting of a DPPH solution and an ethanol solution, and the sample solutions of the embodiment and the comparative example. The results are shown in Table 5.

[0113] Table 5 DPPH free radical scavenging rate test results

[0114]

[0115] The compositions of Examples 1-2 have excellent free radical scavenging effects. In Comparative Examples 5 and 6, the free radical scavenging effects decreased when the plant ratio and enzyme types of the plant enzymatic hydrolysate were changed.

[0116] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition having a moisturizing effect, characterized in that: The invention comprises the following components in percentage by weight: 0.3% to 0.8% of plant fermentation product, 1.2% to 2.0% of plant enzymolysis product, 15% to 20% of moisturizer, 1.6% to 2.5% of Chondrus crispus extract, 0.5% to 1.1% of peony extract, 2.6% to 3.9% of rosemary extract, and the balance is water; The method for preparing the plant fermentation product comprises the following steps: (1) mixing hibiscus leaves, Mirabilis jalapa and Atractylodes lancea and crushing them into particles with a size of less than 100 meshes to obtain a fermentation raw material; (2) mixing fermentation raw materials and water in a weight ratio of 1:(12-14) to obtain a fermentation liquid; adding mixed bacteria to the fermentation liquid to perform anaerobic fermentation at a fermentation temperature of 36-40° C. for 24-30 hours, and sterilizing after the fermentation to obtain a fermentation product; (3) The fermentation product is filtered using a 0.22 μm filter membrane, and the filtrate is subjected to reduced pressure rotary evaporation and dried to obtain a plant fermentation product.

2. The composition having a moisturizing effect according to claim 1, characterized in that: The weight ratio of hibiscus leaves, Mirabilis jalapa and Atractylodes lancea is 1:(0.3-0.5):(1.4-1.6).

3. The composition with moisturizing effect according to claim 1, characterized in that The bacterial mix includes Bifidobacterium lactis, Lactobacillus casei and Lactobacillus helveticus.

4. The composition having a moisturizing effect according to claim 3, characterized in that: The amount of Bifidobacterium lactis in the fermentation broth is 10 8 -10 9 CFU / mL; the amount of Lactobacillus casei in the fermentation broth was 10 8 -10 9 CFU / mL; the amount of Lactobacillus helveticus in the fermentation broth was 10 9 -10 10 CFU / mL.

5. The composition having a moisturizing effect according to claim 1, characterized in that: The method for preparing the plant enzymatic hydrolysate comprises the following steps: (1) mixing radix bupleuri, citron and marigold flowers, and crushing to less than 100 meshes to obtain an enzymatic hydrolysis raw material; (2) mixing the enzymatic hydrolysis raw material and water in a weight ratio of 1:(12-14) to obtain an enzymatic hydrolysis solution; adding cellulase to the enzymatic hydrolysis solution, enzymolyzing at 30-35° C. for 5-8 hours, inactivating the enzyme, adding pectinase, enzymolyzing at 30-35° C. for 3-5 hours, inactivating the enzyme, and finally adding neutral protease, enzymolyzing at 30-35° C. for 4-6 hours, inactivating the enzyme, and vacuum drying to a moisture content of 2-5wt% to obtain an enzymatic hydrolysis product; (3) mixing the enzymatic hydrolysis product with an ethanol aqueous solution having a volume concentration of 65-70%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 220-240 W and 40-45° C. for 2-3 h to obtain an extract; (4) The extract was filtered using a 0.22 μm filter membrane, and the filtrate was evaporated under reduced pressure and dried to obtain a plant enzymatic hydrolysate.

6. The composition having a moisturizing effect according to claim 5, characterized in that: The weight ratio of Bupleurum root, Citron and Calendula officinalis flower is (0.2-0.4):1:(1.4-1.6).

7. The composition having a moisturizing effect according to claim 5, characterized in that: The amount of cellulase used in the enzymatic hydrolysis solution is 100U / mL-120U / mL; the amount of pectinase used in the enzymatic hydrolysis solution is 70U / mL-90U / mL; and the amount of neutral protease used in the enzymatic hydrolysis solution is 130U / mL-150U / mL.

8. The composition having a moisturizing effect according to claim 1, characterized in that: The moisturizing agent comprises 1,3-butylene glycol, allantoin and glycerin in a weight ratio of 1:(1.2-1.5):(0.6-0.8).

9. The method for preparing the composition with moisturizing effect according to any one of claims 1 to 8, characterized in that: The following steps are involved: Add the moisturizer to the dry reaction container, control the temperature at 45-50°C and the speed at 100-120r / min, slowly add water, continue stirring and mixing evenly at the same temperature and speed, cool to 30-35°C, add the remaining components, stir evenly, and cool to room temperature to obtain a composition with a moisturizing effect.

10. Use of the composition with moisturizing effect according to any one of claims 1 to 8 in the preparation of cosmetics.

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