Microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation and preparation method thereof

Abstract

The invention discloses a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation and a preparation method of the microbial fertilizer, and relates to the technical field of fertilizers, the microbial fertilizer comprises 5-10% by weight of a complex microbial inoculant, 50-60% by weight of an auxiliary material, 10-20% by weight of chitosan oligosaccharide, 10-20% by weight of polyglutamic acid, 10-20% by weight of tea polyphenol, 10-20% by weight of an accelerant and 10-20% by weight of amino acid fermentation broth. The weight ratio of the chitosan oligosaccharide is 1-3%, the weight ratio of the polyglutamic acid is 5-8%, the weight ratio of the tea polyphenol is 0.5-1%, the weight ratio of the accelerant is 0.5-1%, the weight ratio of the amino acid fermentation liquor is 30-60%, and the complex microbial inoculants comprise a photosynthetic bacterium group, a lactic acid bacterium group, an actinomycete group, a filamentous fungus group and bacillus subtilis. The accelerant comprises amylase and polyphenol oxidase. The microbial fertilizer provided by the invention has outstanding water-retaining capacity, can greatly improve the water-retaining property of soil when being applied to land, especially in arid regions, is beneficial to normal growth of plants, and can greatly improve the preparation speed, so that the production efficiency can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation and a preparation method thereof. Background Art

[0002] Microbial fertilizers are biological fertilizers made from one or several beneficial microorganisms through industrial culture and fermentation. Microbial fertilizers are usually divided into two categories: one is to increase the supply of plant nutrients through the life activities of the microorganisms contained in it, resulting in improved plant nutritional status and thus increased yields. Its representative variety is bacterial fertilizer; the other is a broad microbial fertilizer. Although it also increases crop yields through the life activities of the microorganisms contained in it, it is not limited to increasing the supply level of plant nutrients, but also includes the secondary metabolites they produce, such as the stimulating effect of hormones on plants. In many planting areas, the soil will have some ability to do so.

[0003] Especially in arid areas, the soil's water retention capacity is poor, which will have a great impact on vegetation growth. In addition, the preparation cycle of ordinary microbial fertilizers is long and the production efficiency is low and needs to be improved. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation and a preparation method thereof, which solves the problems that the soil has poor water retention capacity, thus greatly affecting the growth of vegetation, and that ordinary microbial fertilizers have a long preparation cycle and low production efficiency that needs to be improved.

[0005] The present invention solves the above technical problems through the following technical scheme. The present invention comprises a composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, tea polyphenols, a promoter and an amino acid fermentation broth. The weight proportion of the composite bacterial agent is 5-10%, the weight proportion of the auxiliary materials is 50-60%, the weight proportion of the chitosan oligosaccharide is 1-3%, the weight proportion of the polyglutamic acid is 5-8%, the weight proportion of the tea polyphenols is 0.5-1%, the weight proportion of the promoter is 0.5-1%, and the weight proportion of the amino acid fermentation broth is 30-60%.

[0006] Preferably, the composite bacterial agent includes photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

[0007] Preferably, the promoter comprises amylase and polyphenol oxidase.

[0008] Preferably, the auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

[0009] A method for preparing a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation comprises the following steps:

[0010] S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, prepare a container, put water in the container and heat it to boiling, add chitosan oligosaccharide and stir it thoroughly, let it stand for 5-10 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent;

[0011] S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, ammonium sulfate, tea polyphenols, promoter and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent, auxiliary materials and polyglutamic acid and stir for 15-20 minutes, then pour in amylase and stir for 5 minutes, then pour in tea polyphenols and polyphenol oxidase and stir for 15-20 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

[0012] Preferably, the weight ratio of the composite bacteria, chitosan oligosaccharide, amino acid fermentation broth, urea and salt is 50:200:2000:1:1.

[0013] Compared with the prior art, the beneficial effects of the present invention are:

[0014] 1. The microbial fertilizer provided by the present invention has an outstanding water-retaining capacity. When applied to land, especially in arid areas, it can greatly improve the water-retaining capacity of the soil, which is beneficial to the normal growth of plants.

[0015] 2. The microbial fertilizer provided by the present invention has a fast growth rate of the bacterial flora, which can greatly increase the preparation speed and thus improve the production efficiency. DETAILED DESCRIPTION

[0016] The above and other technical features and advantages of the present invention are described in more detail below.

[0017] Embodiment 1

[0018] The present embodiment provides a technical solution: a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation. The present invention comprises a composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, tea polyphenols, a promoter and an amino acid fermentation liquid. The weight proportion of the composite bacterial agent is 5%, the weight proportion of the auxiliary materials is 50%, the weight proportion of chitosan oligosaccharide is 2%, the weight proportion of polyglutamic acid is 5%, the weight proportion of tea polyphenols is 1%, the weight proportion of the promoter is 1%, and the weight proportion of the amino acid fermentation liquid is 36%.

[0019] Furthermore, the composite bacterial agent includes photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

[0020] Furthermore, the promoter includes amylase and polyphenol oxidase.

[0021] Furthermore, the auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

[0022] A method for preparing a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation comprises the following steps:

[0023] S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, the weight ratio of composite bacterial species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt is 50:200:2000:1:1, prepare a container, put water in the container and heat it to boiling and add chitosan oligosaccharide and stir it thoroughly, let it stand for 5 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent;

[0024] S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, ammonium sulfate, tea polyphenols, promoter and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent, auxiliary materials and polyglutamic acid and stir for 15 minutes, then pour in amylase and stir for 5 minutes, then pour in tea polyphenols and polyphenol oxidase and stir for 15 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

[0025] Control group 1

[0026] The present embodiment provides a technical solution: a microbial fertilizer, which includes a composite bacterial agent, auxiliary materials, chitosan oligosaccharides and amino acid fermentation liquid, wherein the weight proportion of the composite bacterial agent is 5%, the weight proportion of the auxiliary materials is 50%, the weight proportion of chitosan oligosaccharides is 2%, and the weight proportion of the amino acid fermentation liquid is 43%.

[0027] Furthermore, the composite bacterial agent includes photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

[0028] Furthermore, the auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

[0029] A method for preparing microbial fertilizer comprises the following steps:

[0030] S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, the weight ratio of composite bacterial species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt is 50:200:2000:1:1, prepare a container, put water in the container and heat it to boiling and add chitosan oligosaccharide and stir it thoroughly, let it stand for 5 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent;

[0031] S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent and auxiliary materials and stir for 15 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

[0032] Control group 2

[0033] The present embodiment provides a technical solution: a microbial fertilizer, which includes a composite bacterial agent, auxiliary materials, chitosan oligosaccharides, polyglutamic acid and amino acid fermentation liquid, wherein the weight proportion of the composite bacterial agent is 5%, the weight proportion of the auxiliary materials is 50%, the weight proportion of chitosan oligosaccharides is 2%, the weight proportion of polyglutamic acid is 5%, and the weight proportion of the amino acid fermentation liquid is 38%.

[0034] Furthermore, the composite bacterial agent includes photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

[0035] Furthermore, the auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

[0036] A method for preparing microbial fertilizer comprises the following steps:

[0037] S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, the weight ratio of composite bacterial species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt is 50:200:2000:1:1, prepare a container, put water in the container and heat it to boiling and add chitosan oligosaccharide and stir it thoroughly, let it stand for 5 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent;

[0038] S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent, auxiliary materials and polyglutamic acid and stir for 15 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

[0039] Control group three

[0040] The present embodiment provides a technical solution: a microbial fertilizer, which includes a composite bacterial agent, auxiliary materials, chitosan oligosaccharides, tea polyphenols, a promoter and an amino acid fermentation liquid, wherein the weight of the composite bacterial agent is 5%, the weight of the auxiliary materials is 50%, the weight of the chitosan oligosaccharides is 2%, the weight of the tea polyphenols is 1%, the weight of the promoter is 1%, and the weight of the amino acid fermentation liquid is 41%.

[0041] Furthermore, the composite bacterial agent includes photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

[0042] Furthermore, the auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

[0043] Furthermore, the promoter includes amylase and polyphenol oxidase.

[0044] A method for preparing microbial fertilizer comprises the following steps:

[0045] S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, the weight ratio of composite bacterial species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt is 50:200:2000:1:1, prepare a container, put water in the container and heat it to boiling and add chitosan oligosaccharide and stir it thoroughly, let it stand for 5 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent;

[0046] S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, tea polyphenols, promoter and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent and auxiliary materials and stir for 15 minutes, then pour in amylase and stir for 5 minutes, then pour in tea polyphenols and polyphenol oxidase and stir for 15 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

[0047] Take an equal amount of the microbial fertilizer obtained in the above-mentioned Example 1, the microbial fertilizer obtained in the control group 1, the microbial fertilizer obtained in the control group 2 and the microbial fertilizer obtained in the control group 3 as standby, take out an equal amount of microbial fertilizer and place it in an observation dish. It should be noted that the volume of the observation dish occupied by the microbial fertilizer in the observation dish should be less than half of the volume of the observation dish at the beginning. The volume of the microbial flora is observed under a microscope, and the volume is observed every 1 hour until the volume of the microbial flora is reached. The time when the volume of the microbial flora no longer changes is recorded to obtain the following table:

[0048]

[0049] It can be seen from the above table that the time for the bacterial community of the microbial fertilizer obtained in Example 1 and the bacterial community of the microbial fertilizer obtained in the control group 3 to complete the growth is much shorter than the time for the bacterial community of the microbial fertilizer obtained in the control group 1 and the bacterial community of the microbial fertilizer obtained in the control group 2 to complete the growth. Therefore, by adding a promoter, the decomposition rate of chitosan oligosaccharides and tea polyphenols by the bacteria can be greatly accelerated, thereby accelerating the absorption of growth substances by the bacterial community, and then greatly accelerating the growth rate, thereby speeding up the preparation speed.

[0050] Take an equal amount of the microbial fertilizer obtained in the above-mentioned embodiment 1, the microbial fertilizer obtained in the control group 1, the microbial fertilizer obtained in the control group 2 and the microbial fertilizer obtained in the control group 3 as standby, prepare a piece of experimental land, require the same environment in the land, divide the land into four pieces R1, R2, R3 and R4 of the same size, leave sufficient gap space between R1, R2, R3 and R4, spray each piece of land with an equal amount of water, initially test the land appropriately, then evenly sprinkle plant seeds on R1, R2, R3 and R4, and evenly spray the microbial fertilizer obtained in embodiment 1, the microbial fertilizer obtained in the control group 1, the microbial fertilizer obtained in the control group 2 and the microbial fertilizer obtained in the control group 3 on R1, R2, R3 and R4, and test the land moisture every three days without rainfall, and test five times in total to obtain the following table:

[0051]

[0052]

[0053] It can be seen from the above table that the moisture in land R1, the moisture in land R2, the moisture in land R3 and the moisture in land R4 lost 12.0%, 22.6%, 10.4% and 23.2% within 15 days. Therefore, the moisture loss of land R1 and the moisture loss of land R3 after 15 days are much smaller than the moisture loss of land R2 and the moisture loss of land R4 after 15 days. Therefore, land R1 and land R2 have better water retention effects. The addition of polyglutamic acid can greatly improve the water retention capacity of the soil. Therefore, its use in arid areas can further improve the effect of soil remediation.

[0054] The above are only preferred embodiments of the present invention, which are only illustrative and not restrictive. Those skilled in the art understand that many changes, modifications, and even equivalences may be made to the present invention within the spirit and scope defined by the claims, but all of them will fall within the scope of protection of the present invention.

Claims

1. A microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation, characterized in that: The invention comprises a composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, tea polyphenols, a promoter and an amino acid fermentation broth, wherein the weight of the composite bacterial agent is 5-10%, the weight of the auxiliary materials is 50-60%, the weight of the chitosan oligosaccharide is 1-3%, the weight of the polyglutamic acid is 5-8%, the weight of the tea polyphenols is 0.5-1%, the weight of the promoter is 0.5-1%, and the weight of the amino acid fermentation broth is 30-60%.

2. The microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation according to claim 1, characterized in that: The composite bacterial agent comprises photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi and Bacillus subtilis.

3. A microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation as claimed in claim 2, characterized in that: The promoter includes amylase and polyphenol oxidase.

4. The microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation according to claim 1, characterized in that: The auxiliary materials include aldehyde residue, wood ash, egg powder and fungus residue.

5. A method for preparing a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation, characterized in that: The preparation method is applicable to the microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation according to any one of claims 1 to 3, comprising the following steps: S1. Preparation of composite bacterial agent: prepare composite bacterial species including photosynthetic bacterial species, lactic acid bacterial species, actinomycete bacterial species and filamentous fungal species, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt in proportion, prepare a container, put water in the container and heat it to boiling, add chitosan oligosaccharide and stir it thoroughly, let it stand for 5-10 minutes after boiling, add composite bacterial species, urea and salt when the water temperature drops to 40°C and stir for 15 minutes, then seal the container and ferment it, and ferment it for 2 days at a temperature of 35°C to obtain the composite bacterial agent; S2. Preparation of microbial fertilizer: prepare the composite bacterial agent, auxiliary materials, chitosan oligosaccharide, polyglutamic acid, ammonium sulfate, tea polyphenols, promoter and amino acid fermentation liquid according to the ratio, prepare a container, put water in the container and heat it to boiling to eliminate other bacteria in the amino acid fermentation liquid, then pour in chitosan oligosaccharide and stir well, after the water temperature drops to 40°C, pour in the composite bacterial agent, auxiliary materials and polyglutamic acid and stir for 15-20 minutes, then pour in amylase and stir for 5 minutes, then pour in tea polyphenols and polyphenol oxidase and stir for 15-20 minutes, finally pour into a sealed container and let it stand for a period of time at a temperature of 35°C to obtain the microbial fertilizer.

6. The method for preparing a microbial fertilizer containing polyglutamic acid and chitosan oligosaccharide for soil remediation according to claim 5, characterized in that: The weight proportion of the composite bacteria, chitosan oligosaccharide, amino acid fermentation liquid, urea and salt is 50:200:2000:1:1.

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