Method for synergistically improving tomato rhizosphere micro-ecology through combination of pseudomonas morganii and arbuscular mycorrhizal fungi and application of method

Abstract

The invention discloses a method for synergistically improving tomato rhizosphere micro-ecology by utilizing Pseudomonas morganae and arbuscular mycorrhizal fungi and application of the method, a strain HY-S70 belongs to Pseudomonas morganae, and the preservation number of the strain HY-S70 is CGMCC (China General Microbiological Culture Collection Center) No.32673. The invention further discloses a method for synergistically improving tomato rhizosphere micro-ecology by utilizing Pseudomonas morganae and arbuscular mycorrhizal fungi. Before tomato planting, arbuscular mycorrhizal fungi inoculant (preferably physopodium mosseae) is inoculated in soil, and the inoculation amount is 30 spores per gram of inoculant; and after the tomatoes are planted for 3-5 days, a pseudomonas ferrawiae solution with the thallus concentration of 1 * 10 < 9 > CFU / ml is inoculated. According to the combined use method of the pseudomonas morganae and the arbuscular mycorrhizal fungi, phosphorus in soil can be activated, growth of tomato plants is promoted, the rhizosphere microbial community structure of the tomato plants is improved, the rhizosphere micro-ecological environment of the tomato plants is optimized, and the development prospect of large-area application and popularization is achieved.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a method for synergistically improving tomato rhizosphere microecology by utilizing Pseudomonas moravianus in combination with arbuscular mycorrhizal fungi and an application thereof. Background Art

[0002] Tomato is the largest vegetable crop in the world and is popular among consumers because it is rich in nutrients such as vitamin C and organic acids. Greenhouses are the main way to grow tomatoes. However, unreasonable fertilizer input and management measures (such as continuous cropping) not only lead to soil compaction and nutrient imbalance, but also reduce tomato quality indicators such as soluble sugar, affecting the function of its rhizosphere microbial community.

[0003] Microbial agents are a new type of environmentally friendly fertilizer containing a large number of beneficial live bacteria and a variety of natural active substances. When microbial agents are applied to the soil, functional bacteria can promote crop growth and prevent and control soil-borne diseases through multiple mechanisms such as activating nutrients (nitrogen fixation, phosphorus solubilization, potassium solubilization, etc.), producing plant growth hormones, and secreting antibiotic substances. Compared with single inoculation of bacteria, double inoculation of beneficial bacteria and arbuscular mycorrhizal fungi helps to overcome the problems of poor environmental adaptability and unstable effects of single bacteria. On the one hand, the increased diversity of bacterial species makes the spectrum of resources available to the bacterial community wider and enhances its competitiveness; on the other hand, double inoculation helps to integrate the functional advantages of different strains, and has a synergistic effect in promoting plant root growth and improving the soil microecological environment.

[0004] Crop yield is closely related to soil rhizosphere microbial communities. Studies have shown that rhizosphere core microorganisms play a key role in resisting the invasion of soil-borne pathogens and maintaining plant health. Rhizosphere microorganisms not only affect soil material circulation and energy flow, but also promote the formation of soil aggregate structure, increase soil organic matter content and improve soil nutrient circulation. The application of biofertilizers in agricultural production is an important trend in future agricultural development. Using microbial agents or biofertilizers to regulate crop growth can maintain its yield and improve crop quality, which will help promote sustainable agricultural development and achieve the "dual carbon" goals. Summary of the invention

[0005] The purpose of the present invention is to develop a method for improving the rhizosphere microecology of tomatoes by combining Pseudomonas moravica with arbuscular mycorrhizal fungi in order to solve the problems of soil compaction, nutrient imbalance, and reduced microbial diversity in tomato planting soil in agricultural production. The technical solution of the present invention can improve the growth indicators of tomato plants such as plant height and stem diameter, promote the root growth of tomato seedlings, and improve the diversity and community structure of tomato rhizosphere microorganisms.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] In the first aspect, the present invention protects a strain of Pseudomonas moraviensis, named HY-S70, which was deposited in the General Microbiology Center of China National Microbiological Culture Collection Committee on November 21, 2024. The deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is: CGMCC No. 32673.

[0008] The HY-S70 strain was isolated and screened from the rhizosphere of healthy melons collected in Huai'an City, Jiangsu Province on September 23, 2022.

[0009] In a second aspect, the present invention provides a bacterial agent, which also contains the aforementioned Pseudomonas Moravia or a fermentation product containing the aforementioned Pseudomonas Moravia.

[0010] In a specific embodiment, the preparation method of the fermentation product is as follows: the Pseudomonas moraviensis HY-S70 described above is inoculated into LB liquid culture medium for fermentation culture, and the liquid culture conditions are: temperature 28-30°C, rotation speed 170-180rpm, fermentation time 24-36h, to obtain Pseudomonas moraviensis HY-S70 fermentation liquid, the Pseudomonas moraviensis HY-S70 fermentation liquid cell concentration of ≥1×10 9 CFU / mL.

[0011] In a third aspect, the present invention also protects the use of the aforementioned Pseudomonas Moraviae combined with arbuscular mycorrhizal fungi in improving the rhizosphere microecology of tomatoes.

[0012] In a specific embodiment, the present invention also protects the use of the aforementioned Pseudomonas Moraviae in combination with arbuscular mycorrhizal fungi in promoting tomato growth.

[0013] In a fourth aspect, the present invention protects a method for improving the rhizosphere microecology of tomatoes by combining the aforementioned Pseudomonas Moraviae with arbuscular mycorrhizal fungi, comprising the following steps: firstly, mixing the arbuscular mycorrhizal fungi with the planting soil, transplanting the tomato seedlings (three leaves and one heart) for 3 to 5 days, and then adding 30 ml of 1×10 9 CFU / mL of Pseudomonas Moraviae HY-S70 bacterial solution, so that the bacterial concentration reaches 1×10 7 CFU / g or above.

[0014] In a specific embodiment, the present invention also protects a method for promoting tomato growth by using the aforementioned Pseudomonas moravica in combination with arbuscular mycorrhizal fungi.

[0015] There is no special stipulation on the type of the arbuscular mycorrhizal fungi, and there is no special limitation on the source, and conventional commercially available products can be used.

[0016] In a specific embodiment, the arbuscular mycorrhizal fungus is Pipepodius mosseae, preferably Pipepodius mosseae BGC HEB02, which is purchased from the Arbuscular Mycorrhizal Fungi Germplasm Resource Bank of Beijing Academy of Agriculture and Forestry Sciences. Pipepodius mosseae is propagated in sorghum pots before inoculation, and the inoculant includes a soil-sand mixture containing spores, infected root segments or hyphae of Pipepodius mosseae fungus.

[0017] In a more specific embodiment, the Pseudomonas mosseae is prepared into a Pseudomonas mosseae fungicide for use, and 1 gram of the fungicide contains 30 to 40 spores.

[0018] Beneficial Effects

[0019] 1. The present invention screened out a strain of Pseudomonas moravica HY-S70 with the functions of solubilizing phosphate and producing IAA. Compared with other strains, adding the bacterial solution of Pseudomonas moravica HY-S70 into the matrix significantly improved growth indicators such as plant height, stem diameter and aboveground biomass of tomato seedlings, and significantly improved root system structure such as root length, root surface area and number of root tips.

[0020] 2. Through potted experiments, it was found that compared with no application of microbial agents or single application of agents, dual inoculation of Moravia Pseudomonas HY-S70 and arbuscular mycorrhizal fungi synergistically increased the biomass of tomato plants, increased the relative abundance of beneficial microorganisms, and improved the structure of soil rhizosphere microbial communities.

[0021] 3. The present invention provides a method for applying a microbial agent which is easy to operate, environmentally friendly, green and effective, and has a significant promoting effect on the growth of tomato crops.

[0022] Collection Instructions

[0023] Species name: Pseudomonas moravianus;

[0024] Latin name: Pseudomonas moraviensis;

[0025] Strain number: HY-S70;

[0026] Depository: General Microbiology Center, China Microbiological Culture Collection Administration;

[0027] Abbreviation of depository institution: CGMCC;

[0028] Storage address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing;

[0029] Deposit date: November 21, 2024;

[0030] The registration number of the Collection Center is: CGMCC No.32673. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Effects of different bacterial agent treatments on tomato plant height.

[0032] Figure 2 Effects of different bacterial agent treatments on tomato stem diameter.

[0033] Figure 3 Effects of different bacterial agent treatments on the fresh weight of aboveground parts of tomatoes.

[0034] Figure 4 Effects of different bacterial agent treatments on the aboveground dry weight of tomato.

[0035] Figure 5 Effects of different bacterial agent treatments on the richness index of tomato rhizosphere bacterial community.

[0036] Figure 6 Effects of different bacterial agent treatments on the Shannon diversity index of tomato rhizospheric bacterial community.

[0037] Figure 7 Effects of different bacterial agent treatments on the Simpson index of tomato rhizospheric bacterial community.

[0038] Figure 8 Effects of different inoculant treatments on the relative abundance of bacterial phyla in the tomato rhizosphere.

[0039] Fig. 9 Effects of different microbial agent treatments on the relative abundance of bacterial genus levels in the tomato rhizosphere.

[0040] Fig.10 Effects of different microbial agent treatments on the β-diversity of tomato rhizospheric bacterial communities.

[0041] Fig.11 Prediction of the functions of tomato rhizosphere bacterial communities under different microbial agent treatments. DETAILED DESCRIPTION

[0042] The technical solution of the present invention is further described in detail below through specific implementation methods.

[0043] Unless otherwise specified, the terms used in the present invention are commonly used terms in the relevant field. The technical means used in the embodiments, such as strain acquisition, testing methods, etc., are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available.

[0044] The culture medium formula used in the following examples is as follows:

[0045] Pseudomonas selective medium (CFC): Specifically: weigh 49.4g of CFC medium, 10g of glycerol, heat and dissolve in 1L of distilled water, dispense into 200mL bottles, sterilize at 121℃ for 15 minutes, and add one bottle of Pseudomonas CFC selective medium additive to each bottle when cooled to about 50℃, and mix well. Medium formula: 16g of gelatin peptone, 10g of acid hydrolyzed casein, 10g of potassium sulfate, 1.4g of magnesium chloride, 12g of agar, and add 8g of agar per liter of medium before sterilization.

[0046] Inorganic phosphorus medium: glucose 10g / L, ammonium sulfate 0.5g / L, yeast extract powder 0.5g / L, sodium chloride 0.3g / L, potassium chloride 0.3g / L, magnesium sulfate 0.3g / L, ferrous sulfate 0.03g / L, manganese sulfate 0.03g / L, tricalcium phosphate 5g / L, pH adjusted to 7.0-7.5; solid medium: add 15g agar powder to the above ingredients. After packaging, sterilize at 121℃ for 15 minutes and set aside.

[0047] Organophosphorus solution culture medium: glucose 10g / L, ammonium sulfate 0.5g / L, yeast extract powder 0.5g / L, sodium chloride 0.3g / L, potassium chloride 0.3g / L, magnesium sulfate 0.3g / L, ferrous sulfate 0.03g / L, manganese sulfate 0.03g / L, lecithin 0.2g / L, calcium carbonate 1g / L, pH adjusted to 7.0-7.5.

[0048] LB medium: 10 g / L tryptone, 5 g / L yeast extract powder, 10 g / L NaCl, pH adjusted to 7.0-7.2.

[0049] Example 1. Soil sample collection and Pseudomonas isolation and screening

[0050] Acquisition of strains: On September 23, 2022, the rhizosphere soil of healthy melons was collected in Huai'an City, Jiangsu Province for isolation and screening. 10 g of melon rhizosphere soil was collected and placed in a triangular flask containing glass beads and 90 mL of sterile water. The soil suspension was vibrated at 30°C and 170 rpm for 30 min and allowed to stand for 10 min. The soil suspension was gradiently diluted to 10 -3 , 10 -4 , 10 -5 100 μl of the three concentrations were spread on Pseudomonas selective medium (CFC) plates and placed in an incubator at 30°C for inversion culture for 2-5 days. Typical single colonies of different types were picked out and purified on the plates multiple times to obtain a single strain, which was then stored in a -80°C refrigerator in glycerol tubes.

[0051] Example 2: Determination and rescreening of growth-promoting properties of candidate strains

[0052] A total of 115 strains were screened and the strains to be tested were inoculated on inorganic phosphorus culture medium and organic phosphorus culture medium plates and cultured at 30℃ for 3-4 days to observe whether there was a phosphorus-dissolving ring. The strains (a total of 12 strains) that produced a large phosphorus-dissolving ring were quantitatively determined by the molybdenum antimony colorimetric method.

[0053] Molybdenum antimony colorimetric method: inoculate the strain to be tested into LB liquid culture medium for activation, take 5 mL of seed liquid and inoculate into 50 mL of inorganic phosphorus culture medium and organic phosphorus culture medium respectively, use non-inoculated bacteria as control, culture in a shaking incubator at 30°C and 180 rpm for 5 days, take 10 mL of fermentation broth and centrifuge at 10,000 rpm for 10 min, and determine the effective phosphorus content of the supernatant by molybdenum antimony colorimetric method, and measure the pH at the same time.

[0054] Determination of indoleacetic acid production: The activated strain was inoculated into LB liquid medium containing L-tryptophan (200 mg / L), and cultured in a shaking incubator at 30°C and 180 rpm for 3 days. The bacterial suspension was centrifuged at 10,000 rpm for 10 min, and the supernatant was added with an equal volume of Salkowski colorimetric solution, and the mixture was kept in the dark for 30 min to determine its OD530 value. A standard curve was drawn with analytically pure indoleacetic acid, and the content of indoleacetic acid per unit volume of fermentation broth was calculated by comparing the standard curve.

[0055] Iron carrier determination: The strains to be tested were cultured in LB liquid medium on a shaking table for 24 h, 2 μL of the bacterial solution was spotted on CAS solid medium (containing CAS blue detection solution, 30.24 g / L PIPES, and 0.9% agarose), and cultured at 30°C for 3 days. Whether a light yellow or light red halo appeared around the colony was observed to determine its ability to produce siderophore.

[0056] The results are shown in Table 1. Nine strains of phosphate-dissolving bacteria were screened using inorganic phosphorus culture plates. The ratio of the diameter of the phosphate-dissolving circle to the colony diameter on the plate ranged from 2.06 to 3.76, and the D / d values ​​were all greater than 2, indicating that these bacteria had a good effect in dissolving inorganic phosphorus. The amount of phosphorus dissolved in the fermentation broth of all phosphate-dissolving bacteria ranged from 12.45 to 276.61 mg / L, among which the strain HY-S70 had the strongest phosphorus-dissolving ability, followed by the strains HY-L9 and HY-L8. The 12 strains of phosphate-dissolving bacteria screened were shaken and cultured in an organic phosphorus liquid culture medium for 5 days, and it was found that the amount of phosphorus dissolved in the fermentation broth of all strains ranged from 1.83 to 7.3 mg / L, indicating that these strains were better at dissolving inorganic phosphorus than organic phosphorus.

[0057] The quantitative determination results of the IAA production capacity of the strains showed that the IAA production of all strains was greater than 5.00 mg / L, with HY-S70 producing the highest IAA content, reaching 10.77 mg / L. In addition, a total of 9 strains tested had the ability to produce siderophores.

[0058] Table 1 Growth-promoting properties of different strains

[0059]

[0060] Note: From “+++” to “+”, it means the ability to produce siderophore decreases from strong to weak (according to As / Ar); “—” means that the indicator was not observed.

[0061] Example 3: Application of candidate strains in tomato seedling raising experiment

[0062] Preparation of bacterial agent: Four strains with better growth-promoting properties were selected from the 12 strains screened in Example 2, namely HY-L8, HY-L9, HY-S49, and HY-S70. These four strains were inoculated into LB liquid culture medium and cultured at 30°C and 180 rpm for 24 hours to obtain bacterial suspension.

[0063] Preparation of biological seedling medium: Mix the prepared bacterial suspension with ordinary seedling medium at an inoculation amount of 5% (volume ratio) and place it for 1 to 2 days to obtain the biological seedling medium.

[0064] The seedling experiment was conducted in a plastic greenhouse at the Huai'an Academy of Agricultural Sciences Research and Innovation Base. The following treatments were set: HY-L8, HY-L9, HY-S49, and HY-S70. Each treatment had 3 replicates, and each replicate contained 25 seedlings. Before seedling cultivation, tomato seeds (variety: Ayme Fenbawang F1) were soaked and germinated, and seeds with the same whitening were selected for seedling cultivation. After 28 days of seedling cultivation, plant height, stem diameter, SPAD, fresh weight and dry weight of aboveground parts, and root-related indicators of each treatment were measured.

[0065] The results showed (Table 2-3) that the plant height, stem diameter, fresh weight and dry weight of the seedlings bred in the biological seedling matrix containing HY-S70 strain were significantly higher than those of the seedlings bred in the biological seedling matrix containing HY-L8 and HY-S49 strains. The root length and other root system indicators of the seedlings bred in the biological seedling matrix containing HY-S70 strain were significantly higher than those of the other strains.

[0066] Table 2 Effects of different strains on growth indicators of tomato seedlings

[0067]

[0068] Table 3 Effects of different strains on root growth of tomato seedlings

[0069]

[0070] Example 4: Identification of candidate strains

[0071] According to the experimental results in Examples 1-3, strain HY-S70 was selected for identification and subsequent potted plant application test.

[0072] Identification of strains: Total DNA of strain HY-S70 was extracted using a bacterial total DNA extraction kit. Universal primers 27F / 1492R were used for 16S rDNA amplification, forward primer 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; reverse primer 1492R: 5'-GGTTACCTTGTTACGACTT-3'. The sequencing results were compared by BLAST homology in the database of the National Center for Biotechnology Information (NCBI), and the sequences with high homology and representativeness were selected for download. Multiple sequence alignment was performed using the built-in ClustalW command of MEGA 11.0. After removing the intervals, the optimal nucleic acid substitution model was found to be Kimura 2+Gamma distribution through automatic analysis. Based on this model, a maximum likelihood evolutionary tree with a Bootstrap of 500 was constructed, and the evolutionary tree was made using FigTree 1.4.4. The results showed that strain HY-S70 was in the same branch as Pseudomonas moraviensis, with the highest similarity. Finally, strain HY-S70 was identified as Pseudomonas moraviensis.

[0073] Example 5: Application of Pseudomonas combined with arbuscular mycorrhizal fungi in tomato potted plant experiment

[0074] Preparation of Pseudomonas inoculum: strain HY-S70 was inoculated into LB liquid medium and cultured in a shaking incubator at 30°C and 180 rpm for 24 h. After the culture was completed, the OD value was adjusted with sterile water so that the concentration of each bacterial suspension was between 10 9 CFU / ml, for future use.

[0075] Preparation of arbuscular mycorrhizal fungal inoculant: Funneliformis mosseae BGCHEB02 was selected and purchased from the Arbuscular Mycorrhizal Fungal Germplasm Resource Bank of Beijing Academy of Agricultural and Forestry Sciences. Funneliformis mosseae was propagated in sorghum pots before inoculation, and the propagation medium was river sand and soil. Two months after planting sorghum, it was inoculated into the pot soil of tomato. The inoculant included a soil-sand mixture, spores, infected root segments or hyphae of Funneliformis mosseae fungi, and 1g of the inoculant contained 30 to 40 spores.

[0076] Implementation plan for potted plant experiment: A total of 4 treatments were set up, namely CK (without fungicide), HY-S70, AMF, and HY-S70+AMF. Each pot was filled with 7kg of soil, and each treatment was repeated 6 times. Before transplanting, the arbuscular mycorrhizal fungal agent was mixed with the planting soil, and the inoculation amount was added according to 1.5% of the dry soil weight. Commercial organic fertilizer was added to each treatment at 2% of the dry soil weight. Tomato seedlings (three leaves and one heart) with consistent growth were selected for transplanting. After 3 to 5 days of transplanting, the Moravian Pseudomonas HY-S70 bacterial solution was added to the tomato root area. After 30 days of transplanting, the growth indicators of tomato plants and the structure of the rhizosphere microbial community of each treatment group were measured.

[0077] The results are as follows Figures 1 to 4 As shown, the tomato plant height under the treatment of compound bacterial agent HY-S70+AMF increased by 24.37% compared with the HY-S70 treatment group, 36.14% compared with the AMF treatment group, and 65.60% compared with the blank group;

[0078] The stem diameter of tomatoes treated with the composite fungus HY-S70+AMF increased by 18.15% compared with the HY-S70 treatment group, 35.06% compared with the AMF treatment group, and 23.77% compared with the blank group.

[0079] The fresh weight of aboveground part of tomatoes treated with composite fungus HY-S70+AMF increased by 33.95% compared with HY-S70 treatment group, 52.50% compared with AMF treatment group, and 69.20% compared with blank group.

[0080] The stem diameter of tomatoes treated with the composite fungus HY-S70+AMF increased by 26.46% compared with the HY-S70 treatment group, 39.20% compared with the AMF treatment group, and 83.57% compared with the blank group.

[0081] In summary, the plant height, stem diameter, aboveground fresh weight and aboveground dry weight of tomatoes treated with the composite fungus HY-S70+AMF were significantly higher than those in other treatment groups.

[0082] Example 6: Application of Pseudomonas combined with arbuscular mycorrhizal fungi in improving tomato rhizosphere microbial community

[0083] Determination of rhizosphere microbial community in tomato: 0.5 g of rhizosphere soil samples were collected from the CK control group and the HY-S70+AMF treatment group, and total DNA was extracted using Qubit 3.0 according to the instructions. The quality of DNA was detected by NanoDrop2000 and 1% agarose gel electrophoresis, and the concentration and purity were determined. The primer sequences were 341F and 806R for 16S rDNA amplification, forward primer 341F: 5'-CCTACGGGNGGCWGCAG-3'; reverse primer 806R: 5'-GGACTACHVGGGTATCTAAT-3'. Amplification system: 4 μL of 5× amplification buffer, 0.8 μL of upstream and downstream primers (5 μmol / L), 2 μL of 2.5 mmol / L dNTPs, 0.4 μL of TransStart FastPfu polymerase, 10 ng of template DNA, and 20 μL of complement. Procedure: pre-denaturation 95℃3min, 95℃30s, 55℃30s, 72℃30s, 72℃10min, 27 cycles, stored at 4℃. PCR products were purified using AMPure XP Beads and quantified using Qubit 3.0. Sequencing libraries were constructed using Illumina DNA Prep Kit (Illumina, CA, USA). Library quality was tested using ABI StepOnePlus Real-Time PCR System (Life Technologies, USA), and sequencing was performed using Novasek 6000 PE250 mode pooling (NovaSeq6000 S2 Reagent Kit v1.5, Illumina, USA). Alpha diversity and beta diversity indices were calculated using the Kidio Bio Cloud Platform software.

[0084] The results are as follows Figures 5 to 11 As shown, compared with the control CK treatment, the tomato rhizosphere dominance index and Shannon diversity index of the group treated with the compound bacterial agent HY-S70+AMF increased significantly;

[0085] In terms of the relative abundance of rhizospheric bacteria at the phylum level, the relative abundance of Proteobacteria, Firmicutes, Actinobacteria and Gemmatimonadetes in the treatment with the compound bacterial agent HY-S70+AMF was significantly increased compared with the CK treatment; in terms of the relative abundance at the genus level, the relative abundance of Pseudomonas, Staphylococcus and Corynebacterium in the treatment with the compound bacterial agent HY-S70+AMF was significantly increased compared with the CK treatment.

[0086] Non-metric multidimensional scaling (NMDS) was used to analyze the distance of microbial communities and characterize β diversity. At the genus level, the stress function value was 0.029, which was less than 0.05, and it could well reveal the differences in bacterial community composition between different treatments. It was preliminarily judged that the inoculation of composite flora affected the composition of bacterial communities.

[0087] The results of functional prediction analysis showed that there were significant differences in the predicted functions of rhizosphere microbial communities between the HY-S70+AMF treatment and the CK treatment. For example, the relative abundance of amino acid metabolism, coenzyme and vitamin metabolism, carbohydrate metabolism and polyketide metabolism of the rhizosphere bacterial community in the HY-S70+AMF treatment soil were significantly higher than those in the CK soil.

[0088] In summary, the present invention provides a method for co-application of Pseudomonas moravianus and arbuscular mycorrhizal fungi, which synergistically promotes tomato growth and improves the diversity and community composition of bacteria in the tomato rhizosphere soil.

[0089] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. The general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the broadest scope consistent with the principles disclosed herein and novel specific embodiments.

Claims

1. A strain of Pseudomonas moravianus HY-S70, classified and named Pseudomonas moravianus (Pseudomonas moravianus), was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on November 21, 2024. The deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is: CGMCC No. 32673.

2. A bacterial agent, comprising the Pseudomonas moravica HY-S70 according to claim 1 or a fermentation product thereof.

3. The bacterial agent according to claim 2, characterized in that The fermentation product is obtained by inoculating the Moravian Pseudomonas HY-S70 described in claim 1 into LB liquid culture medium for fermentation culture. The liquid culture conditions are: temperature 28-30°C, rotation speed 170-180rpm, fermentation time 24-36h, to obtain Moravian Pseudomonas HY-S70 fermentation liquid, and the cell concentration of Moravian Pseudomonas HY-S70 fermentation liquid is ≥1×10 9 CFU / mL.

4. Use of Pseudomonas moravica HY-S70 combined with arbuscular mycorrhizal fungi as described in claim 1 in improving the rhizosphere microecology of tomatoes.

5. A method for improving the microecology of tomato rhizosphere by combining Pseudomonas moravianus HY-S70 and arbuscular mycorrhizal fungi according to claim 1, comprising the following steps: firstly, mixing the arbuscular mycorrhizal fungi with the planting soil, and then, 30 ml of 1×10 9 CFU / mL of Pseudomonas Moraviae HY-S70 bacterial solution, so that the bacterial concentration reaches 1×10 7 CFU / g or above.

6. The use according to claim 4 or the method according to claim 5, characterized in that: The arbuscular mycorrhizal fungus is Pseudomonas mosseae.

7. The use according to claim 6 or the method according to claim 6, characterized in that The described Tubulostomyces mosseae is Tubulostomyces mosseae BGC HEB02, which was purchased from the Arbuscular Mycorrhizal Fungi Germplasm Resource Bank of Beijing Academy of Agriculture and Forestry Sciences.

8. The use according to claim 6 or the method according to claim 6, characterized in that: Before inoculation, the fungus was propagated in sorghum pots in advance, and the inoculant included a soil-sand mixture containing spores, infected root segments or hyphae of the fungus.

9. The use according to claim 6 or the method according to claim 6, characterized in that: The mossea spores are made into mossea spores fungal agent for use, and 1 gram of the fungal agent contains 30 to 40 spores.

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