Plant pathogenic fungus monitoring method integrating eDNA and qPCR technology and application

Through the integration of eDNA and qPCR technology, combined with specific primer pairs and probes, efficient and accurate detection of plant pathogenic fungi is achieved, and the problems of low detection accuracy and affected by sample abundance in the prior art are solved, and are suitable for monitoring of a variety of environmental samples.

CN119955911APending Publication Date: 2025-05-09INST OF MICROBIOLOGY CHINESE ACAD OF SCI +1
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Patent Information

Application Number
CN202510129040.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-05
Publication Date
2025-05-09

AI Technical Summary

Technical Problem

When monitoring plant pathogenic fungi, the prior art is susceptible to the abundance of the detection target in the overall sample, and due to the short fragments and relatively low accuracy, it is difficult to achieve efficient and large-scale biodiversity monitoring.

Method used

The monitoring method of integrated eDNA and qPCR technology is used to perform initial screening through specific primer pairs and probes combined with eDNA macrobarcoding technology, and then accurately identify and quantitatively determine using qPCR to achieve efficient and accurate detection of plant pathogenic fungi.

Benefits of technology

The process of initial screening and accurate identification of plant pathogenic fungi is realized, and its initial DNA concentration can be quantitatively detected, which improves the accuracy and efficiency of monitoring, and is suitable for the detection of environmental samples such as air, water, and soil.

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Abstract

The invention belongs to the technical field, and particularly relates to a plant pathogenic fungus monitoring method integrating eDNA and qPCR technologies and application, plant pathogenic fungi can be efficiently and accurately detected and recognized from an environmental sample by combining the steps of sample collection, sample DNA extraction, DNA macro bar code monitoring, TaqMan fluorescent quantitative qPCR detection and the like, and the method has the advantages that the method is simple and convenient to operate and high in practicability. A new tool and an important process are provided for environment monitoring and plant disease prevention and control. According to the method, plant diseases can be quickly responded, measures can be taken in time, agricultural production loss is reduced, and the ecological environment is protected.
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Citation Information

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