A keratin YK93-9, its preparation method, pharmaceutical composition thereof, and its uses

By designing the amino acid sequence and nucleic acid molecule of keratin YK93-9, and expressing and purifying it in host cells using an expression vector, the problem of difficult keratin extraction was solved, achieving high-yield and high-purity keratin preparation and therapeutic effects for various diseases.

CN119968384BActive Publication Date: 2026-05-26INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
Filing Date
2023-09-26
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Keratin is insoluble in various solvents and resistant to enzymatic hydrolysis, making it difficult to extract and utilize effectively. Existing technologies have not yielded any research on the preparation and application of keratin YK93-9.

Method used

A keratin YK93-9 drug composition was prepared by designing the amino acid sequence (SEQ ID NO.1) of keratin YK93-9, encoding a nucleic acid molecule (SEQ ID NO.2), expressing it in host cells (such as Escherichia coli BL21(DE3)) using an expression vector (such as the pET series), and purifying it by methods such as ultrafiltration, dialysis, column chromatography, and salting out.

Benefits of technology

Achieving high yield and high purity of keratin YK93-9 was demonstrated, showing significant therapeutic effects on diseases such as benign prostatic hyperplasia, lymphoma, lung cancer, and melanoma, and possessing potential for application as an antipyretic, analgesic, antiepileptic, and anti-inflammatory drugs.

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Abstract

This invention belongs to the field of biopharmaceuticals and provides a keratin YK93-9, its encoded nucleic acid molecule, an expression vector containing the nucleic acid molecule, a host cell containing the expression vector or whose genome integrates the nucleic acid molecule, as well as a preparation method and a pharmaceutical composition thereof. It also provides the application of the above-mentioned keratin YK93-9 and other substances in the preparation of drugs for treating lung cancer, lymphoma, breast cancer, melanoma, uterine fibroids, benign prostatic hyperplasia, etc.
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