Application of abundance branch element or analogue thereof as plant growth regulator

Abstract

The invention relates to an application of Fengfu element or an analogue thereof as a plant growth regulator, and belongs to the technical field of plant growth regulators. In order to screen plant growth regulators from microorganisms, the invention provides a plant growth regulator containing an aqueous solution of the abundance branch element or the abundance branch element analogue, and the concentration of the abundance branch element or the abundance branch element analogue in the plant growth regulator is 15 mu g / L-1500 mu g / L. A seed germination test, a pot experiment and a field experiment are adopted to research the influence of the Fengzhu element and the analogues thereof on plant growth and development, and the Fengzhu element and the analogues thereof are found to be capable of promoting germination of rice, corn and soybean seeds, increasing tillering and yield of rice, promoting growth of seedlings of cucumbers, tomatoes, pakchoi, spinach and lettuces and improving the yield of the seedlings of the cucumbers, the tomatoes, the pakchoi, the spinach and the lettuces. The plant growth regulator can be used as a novel plant growth regulator with high efficiency, low toxicity and low cost, and has development and utilization values and application prospects in agricultural production.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically relates to the application of fengzhisu or its analogs as plant growth regulators. Background Art

[0002] Plant growth regulators play a vital role in modern agriculture and horticulture. They can regulate the growth and development of plants and improve crop yield and quality. Traditional plant growth regulators are mostly prepared by chemical synthesis methods, but with the development of biotechnology, plant growth regulators from microbial sources have gradually attracted attention. Microorganisms have rich diversity and metabolic pathways and can produce a variety of substances with plant growth regulating activity. These substances have the advantages of high efficiency, low toxicity, and environmental friendliness, providing a new way for sustainable agricultural development.

[0003] Fengzhisu, also known as anisic acid, has the chemical name 4-methoxybenzoic acid. It is an important organic synthesis intermediate and modifier. It is also an important raw material in the fields of preservation and pharmaceuticals. It is widely used in the medical, food and daily chemical industries. For example, anisic acid can participate in the synthesis of methyl anisate and ethyl anisate. Methyl anisate and ethyl anisate are both important raw materials for the preparation of flavors, toothpastes and dentifrice. 2-Methoxybenzoic acid and 3-methoxybenzoic acid are both analogs of anisic acid. Among them, 2-methoxybenzoic acid, also known as o-methoxybenzoic acid, can be used in organic synthesis, preservation and disinfection, fragrances, medicine and other fields; 3-methoxybenzoic acid, also known as m-methoxybenzoic acid, can be used as pesticides and pharmaceutical intermediates. However, there are no reports in the existing technology about the use of Fengzhisu or its analogs as plant growth regulators. Preliminary studies in this laboratory found that Fengzhisu can be isolated from the metabolites of endophytic fungi in ice flowers, and has low toxicity. The acute toxicity is manifested as subcutaneous LD in mice. 50 =400mg / kg, rabbit oral LD 50 >5000mg / kg. Therefore, it would be of great significance if Fengzhisu or its analogs could be used as plant growth regulators. Summary of the invention

[0004] In order to screen plant growth regulators from microbial sources, the present invention isolated a compound with plant growth-promoting properties from the metabolites of endophytic fungi in ice flowers in the early stage, and named it Fengzhisu. After identification of Fengzhisu, it was found to be anisic acid (4-methoxybenzoic acid); then the present invention studied the plant growth-promoting properties of Fengzhisu analogue 1 (2-methoxybenzoic acid) and Fengzhisu analogue 2 (3-methoxybenzoic acid), and found that both of them also have plant growth-promoting properties. Based on the above research results, the present invention provides the use of Fengzhisu or its analogues as plant growth regulators, and the specific technical scheme is as follows:

[0005] The first object of the present invention is to provide an application of fengzhisu or its analogues as an effective ingredient of a plant growth regulator in promoting the germination of rice seeds, wherein the application uses an aqueous solution of fengzhisu or an aqueous solution of fengzhisu analogue 1 or an aqueous solution of fengzhisu analogue 2 with a concentration of 15-300 μg / L as a plant growth regulator to soak rice seeds; the fengzhisu is 4-methoxybenzoic acid, the fengzhisu analogue 1 is 2-methoxybenzoic acid, and the fengzhisu analogue 2 is 3-methoxybenzoic acid.

[0006] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0007] In one embodiment of the present invention, the promoting rice seed germination refers to increasing the germination rate, sprout length and root length of the rice seeds.

[0008] Preferably, the concentration of the aqueous solution of fengzhisu or the aqueous solution of fengzhisu analog 1 or the aqueous solution of fengzhisu analog 2 is 150 μg / L.

[0009] The second object of the present invention is to provide an application of Fengzhisu or its analogs as an effective ingredient of a plant growth regulator in improving the cold resistance of rice seeds, wherein the application uses a Fengzhisu aqueous solution or a Fengzhisu analog 1 aqueous solution or a Fengzhisu analog 2 aqueous solution with a concentration of 15-300 μg / L as a plant growth regulator to soak rice seeds to promote the germination of rice seeds under low temperature conditions, wherein the low temperature is 15-18°C; the Fengzhisu is 4-methoxybenzoic acid, the Fengzhisu analog 1 is 2-methoxybenzoic acid, and the Fengzhisu analog 2 is 3-methoxybenzoic acid.

[0010] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0011] In one embodiment of the present invention, the promoting the germination of rice seeds under low temperature conditions refers to increasing the germination rate, sprout length and root length of rice seeds under low temperature conditions.

[0012] Preferably, the concentration of the aqueous solution of fengzhisu or the aqueous solution of fengzhisu analog 1 or the aqueous solution of fengzhisu analog 2 is 150 μg / L.

[0013] The third object of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in promoting rice development, wherein the application uses a Fengzhisu aqueous solution with a concentration of 150 μg / L as a plant growth regulator to soak rice seeds to promote the growth and tillering of rice at different growth stages; the Fengzhisu is 4-methoxybenzoic acid.

[0014] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0015] In one embodiment of the present invention, promoting the growth and tillering of rice at different growth stages refers to increasing the plant height of rice at the seedling stage, tillering stage and heading stage, and increasing the number of tillers at the tillering stage, heading stage and maturity stage.

[0016] The fourth object of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in improving rice yield, wherein the application uses an aqueous solution of Fengzhisu with a concentration of 150 μg / L as a plant growth regulator to soak rice seeds; the Fengzhisu is 4-methoxybenzoic acid.

[0017] In one embodiment of the present invention, the seed soaking is performed at room temperature for 48 hours.

[0018] In one embodiment of the present invention, the increasing of rice yield is to increase the number of effective panicles per square meter, the number of grains per panicle, the number of filled grains, the thousand-grain weight and the yield per mu of rice.

[0019] The fifth object of the present invention is to provide an application of Fengzhisu as an active ingredient of a plant growth regulator in promoting the germination of grain crop seeds, wherein the application uses a seed coating agent with a Fengzhisu concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds; the Fengzhisu is 4-methoxybenzoic acid.

[0020] In one embodiment of the present invention, the food crops include corn and soybean.

[0021] In one embodiment of the present invention, the seed coating agent is a mixture of a 1500 μg / L Fengzhisu aqueous solution and a “Dingmiaoxin” 4.23% metalaxyl·meconazole microemulsion (containing 1.88% metalaxyl and 2.35% meconazole) in a volume ratio of 1:9.

[0022] In one embodiment of the present invention, the promoting of grain crop seed germination refers to increasing the germination rate, sprout length and root length of grain crop seeds.

[0023] The sixth object of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seeds, wherein the application uses a seed coating agent with a Fengzhisu concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds to promote the germination of grain crop seeds under low temperature conditions, wherein the low temperature is 15-18°C; the Fengzhisu is 4-methoxybenzoic acid.

[0024] In one embodiment of the present invention, the food crops include corn and soybean.

[0025] In one embodiment of the present invention, the promoting the germination of grain crop seeds under low temperature conditions refers to increasing the germination rate, sprout length and root length of grain crop seeds under low temperature conditions.

[0026] The seventh object of the present invention is to provide the use of the above-mentioned Fengzhisu as an active ingredient of a plant growth regulator in promoting the growth of grain crop seedlings, wherein the application uses a seed coating agent with a Fengzhisu concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds; the Fengzhisu is 4-methoxybenzoic acid.

[0027] In one embodiment of the present invention, the food crops include corn and soybean.

[0028] In one embodiment of the present invention, the promoting the growth of food crop seedlings refers to increasing the plant height and root length of the food crop seedlings.

[0029] The eighth object of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seedlings, wherein the application uses a seed coating agent with a Fengzhisu concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds to promote the growth of grain crop seedlings under low temperature conditions; the Fengzhisu is 4-methoxybenzoic acid.

[0030] In one embodiment of the present invention, the food crops include corn and soybean.

[0031] In one embodiment of the present invention, promoting the growth of food crop seedlings under low temperature conditions refers to increasing the plant height and root length of food crops under low temperature conditions.

[0032] The ninth objective of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in promoting the growth of fruit vegetable seedlings, wherein a Fengzhisu aqueous solution with a concentration of 1500 μg / L is used as a plant growth regulator to spray the vegetable seedlings; the Fengzhisu is 4-methoxybenzoic acid.

[0033] In one embodiment of the present invention, the fruit vegetables include but are not limited to cucumbers and tomatoes.

[0034] In one embodiment of the present invention, the promoting the growth of fruit vegetable seedlings refers to increasing the plant height and root length of the fruit vegetable seedlings.

[0035] The tenth object of the present invention is to provide an application of Fengzhisu as an effective ingredient of a plant growth regulator in promoting the growth of leafy vegetable seedlings, wherein a Fengzhisu aqueous solution with a concentration of 1500 μg / L is used as a plant growth regulator to spray the vegetable seedlings; the Fengzhisu is 4-methoxybenzoic acid.

[0036] In one embodiment of the present invention, the leafy vegetables include but are not limited to Chinese cabbage, spinach and lettuce.

[0037] In one embodiment of the present invention, the promoting the growth of leafy vegetable seedlings refers to increasing the leaf area of ​​the leafy vegetable seedlings.

[0038] Beneficial effects of the present invention:

[0039] The present invention finds that an aqueous solution of Fengzhisu (4-methoxybenzoic acid) or its analogs (2-methoxybenzoic acid or 3-methoxybenzoic acid) can be used as a plant growth regulator to regulate the growth and development of food crops, oil crops and vegetables. The specific effects are as follows:

[0040] The present invention finds through seed germination experiments that soaking rice seeds with an aqueous solution of fengzhisu or its analogs can promote the germination of rice seeds under normal temperature and low temperature stress conditions, specifically by improving the germination rate, bud length and root length of rice seeds; coating corn or soybean seeds with a coating agent containing an aqueous solution of fengzhisu can promote the germination of corn or soybean seeds under normal temperature and low temperature stress conditions, specifically by improving the germination rate, bud length and root length of rice seeds.

[0041] The present invention finds through indoor and outdoor potted plant experiments and field experiments that soaking rice seeds with an aqueous solution of Fengzhisu can increase the plant height and tillering number of rice at different growth stages, and increase the number of effective ears per square meter, the number of grains per ear, the number of filled grains, the thousand-grain weight and the yield per mu of rice; coating corn or soybean seeds with a coating agent containing the aqueous solution of Fengzhisu can promote the growth of corn or soybean seedlings under normal temperature and low temperature stress conditions, which is specifically manifested in increasing the plant height and root length of the soybean or corn seedlings.

[0042] In addition, the present invention also found that spraying vegetable seedlings with an aqueous solution of Fengzhisu can promote the growth of vegetable seedlings, specifically by increasing the plant height and root length of cucumber or tomato seedlings, and increasing the leaf area of ​​Chinese cabbage, spinach or lettuce seedlings.

[0043] In summary, the aqueous solution of Fengzhisu or its analogues provided by the present invention has the effects of promoting plant seed germination and plant growth, increasing plant tillering or branching, and improving plant yield and stress resistance, and can be used as a new type of plant growth regulator with high efficiency, low toxicity and low cost in agricultural production, and has development and utilization value and application prospects in agricultural production. The present invention provides a research basis for its next step of registration application, batch production and large-scale agricultural use, and has important practical significance. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is the result of soaking rice seeds in 150μg / L Fengzhisu aqueous solution on the germination of rice seeds under laboratory low temperature conditions;

[0045] Figure 2This is a graph showing the effect of soaking seeds in 150 μg / L Fengzhisu aqueous solution on the growth status of different rice varieties at the tillering and maturity stages in an outdoor pot experiment; Figure 2 A in the figure is the growth state diagram of Longjing 31 rice at the tillering stage. Figure 2 B is the growth status diagram of Longdao 20 rice in the tillering stage. Figure 2 C in the figure is the growth state diagram of Longjing 31 rice at maturity. Figure 2 D in the figure is the growth status diagram of Longdao 20 rice at the maturity stage;

[0046] Figure 3 This is a graph showing the effect of soaking seeds in 150 μg / L Fengzhisu aqueous solution on the growth status of Longjing 31 rice at different growth stages in a field experiment; Figure 3 A in the figure is the growth state diagram of rice in the seedling stage. Figure 3 B in the figure is the growth state diagram of rice in the tillering stage. Figure 3 C in the figure is the growth status diagram of rice at heading stage. Figure 3 D in the figure is the growth state diagram of rice at maturity;

[0047] Figure 4 This is the result of the effect of 150μg / L Fengzhisu aqueous solution coating on the growth state of corn in the normal temperature / low temperature pot experiment in the laboratory; Figure 4 A in the figure is the growth state of corn after 14 days of cultivation at room temperature. Figure 4 B is the growth status of corn after 21 days of low-temperature cultivation;

[0048] Figure 5 This is the result of the effect of 150μg / L Fengzhisu aqueous solution coating on the growth status of soybeans in normal temperature / low temperature pot experiments in the laboratory; Figure 5 A in the figure is the growth state of soybean after 14 days of cultivation at room temperature. Figure 5 Figure B shows the growth status of soybeans after 21 days of low-temperature cultivation. DETAILED DESCRIPTION

[0049] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific implementation methods and the accompanying drawings of the specification. The embodiments described in the present invention are not all embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] The experimental methods used in the following examples are all conventional methods unless otherwise specified, and the materials, reagents and instruments used are all conventional materials, reagents and instruments in the art unless otherwise specified, and can be obtained by those skilled in the art through commercial channels.

[0051] The Fengzhisu of the present invention is also known as anisic acid or 4-methoxybenzoic acid, and its chemical structure is shown in Formula 1; the Fengzhisu analogue 1 of the present invention is also known as 2-methoxybenzoic acid, and its chemical structure is shown in Formula 2; the Fengzhisu analogue 2 of the present invention is also known as 3-methoxybenzoic acid, and its chemical structure is shown in Formula 3;

[0052]

[0053] Example 1: Application of Fengzhisu in promoting rice seed germination at room temperature or low temperature in the laboratory by soaking seeds

[0054] The application described in this embodiment is to soak rice seeds in an aqueous solution of Fengzhisu with a concentration of 15 μg / L-300 μg / L, soak them at room temperature for 48 hours, and then place the soaked rice seeds in a laboratory under normal temperature or low temperature stress environment for germination experiment. The specific steps are as follows:

[0055] (1) Weigh 300 μg of fengzhisu, dissolve it in deionized water, and make up to 1 L to prepare a 300 μg / L stock solution.

[0056] (2) Take different volumes of the mother solution in step (1), add deionized water according to the proportion, and dilute to aqueous solutions of Fengzhisu with different concentrations between 15 μg / L and 300 μg / L. The concentrations are shown in Table 1.

[0057] (3) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in Fengzhisu aqueous solution with different concentration gradients for 48 h.

[0058] (4) The soaked rice seeds were transferred to a 9-cm diameter petri dish covered with sterilized filter paper. Ten rice seeds were placed in each petri dish. 5 mL of sterilized deionized water was added to each petri dish. The process was repeated 6 times. The petri dishes were placed in a dark environment at room temperature (28°C) for 4 days and in a dark environment at low temperature (15°C) for 7 days. The germination of the rice seeds was observed and the root length and sprout length of the rice were counted. The results are shown in Tables 1 and Figure 1 shown.

[0059] Table 1 Statistical results of the effects of soaking rice seeds in aqueous solution of different concentrations of Fengzhisu at room temperature / low temperature on rice seed germination

[0060]

[0061]

[0062] From the data shown in Table 1, it can be seen that soaking rice seeds with different concentrations of Fengzhisu aqueous solution can promote rice seed germination, increase rice seed germination rate, and improve the cold resistance of rice seeds. In the normal temperature germination experiment, the best germination promotion effect was achieved when the concentration of Fengzhisu aqueous solution was 150 μg / L. Compared with the normal temperature control, the germination rate, root length, and bud length increased by 5.45%, 10.07%, and 11.00%, respectively; in the low temperature stress germination experiment, the best germination promotion effect was achieved when the concentration of Fengzhisu aqueous solution was 150 μg / L. Compared with the low temperature control, the germination rate, root length, and bud length increased by 6.18%, 20.23%, and 13.74%, respectively.

[0063] Example 2: Application of Fengzhisu analogs to promote rice seed germination by soaking seeds at room temperature or low temperature in the laboratory

[0064] The application described in this embodiment is to soak rice seeds in aqueous solutions of 150 μg / L fengzhisu analogue 1 and fengzhisu analogue 2, respectively, at room temperature for 48 hours, and then place the soaked rice seeds in a laboratory under normal temperature or low temperature stress environment for germination experiment. The specific steps are as follows:

[0065] (1) Weigh 150 μg of fengzhisu analogue 1 and 150 μg of fengzhisu analogue 2, dissolve them in deionized water and adjust the volume to 1 L, respectively, to prepare an aqueous solution of fengzhisu analogue 1 with a concentration of 150 μg / L and an aqueous solution of fengzhisu analogue 2 with a concentration of 150 μg / L.

[0066] (2) Select Longjing 31 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in the aqueous solution of the above-mentioned fengzhi analogue 1 and the aqueous solution of the fengzhi analogue 2 for 48 h, respectively. For the blank control, soak the seeds in deionized water.

[0067] (3) The soaked rice seeds were transferred to a 9 cm diameter petri dish covered with sterilized filter paper, 10 rice seeds were placed in each petri dish, 5 mL of sterilized deionized water was added to each petri dish, and the process was repeated 6 times. The petri dishes were placed in a dark environment at room temperature (28°C) for 4 days and in a dark environment at low temperature (15°C) for 7 days, and the germination of the rice seeds was observed, and the root length and sprout length of the rice were counted. The results are shown in Table 2.

[0068] Table 2 Statistical results of the effects of soaking rice seeds in aqueous solution of Fengzhisu analogue 1 and Fengzhisu analogue 2 at room temperature / low temperature on rice seed germination

[0069]

[0070] As shown in Table 2, soaking rice seeds with an aqueous solution of Fengzhisu analogue 1 can not only promote rice seed germination and improve rice seed germination rate, but also enhance the cold resistance of seeds under low temperature stress conditions. Specifically, under normal temperature conditions, compared with the normal temperature control, the germination rate, root length and sprout length of rice seeds treated with the aqueous solution of Fengzhisu analogue 1 increased by 3.57%, 7.65% and 7.10%, respectively; under low temperature conditions, compared with the low temperature control, the germination rate, root length and sprout length of rice seeds treated with the aqueous solution of Fengzhisu analogue 1 increased by 3.70%, 7.97% and 8.53%, respectively. Soaking rice seeds in an aqueous solution of Fengzhisu analogue 2 can not only promote the germination of rice seeds and improve the germination rate of rice seeds, but also enhance the cold resistance of seeds under low temperature stress conditions. Specifically, under normal temperature conditions, compared with the normal temperature control, the germination rate, root length and sprout length of rice seeds treated with the aqueous solution of Fengzhisu analogue 2 increased by 3.57%, 10.04% and 9.69% respectively; under low temperature conditions, compared with the low temperature control, the germination rate, root length and sprout length of rice seeds treated with the aqueous solution of Fengzhisu analogue 2 increased by 5.56%, 12.65% and 10.68% respectively.

[0071] Example 3: Application of Fengzhisu in increasing the number of rice tillers and yield by soaking seeds in an outdoor pot experiment

[0072] The application described in this embodiment is to soak the rice seeds of Longjing 31 and Longdao 20 in an aqueous solution of Fengzhisu with a concentration of 150 μg / L, soak them at room temperature for 48 hours, and then sow the soaked rice seeds in pots for outdoor rice pot experiments. The specific steps are as follows:

[0073] (1) Weigh 150 μg of fengzhisu, dissolve it in deionized water, and adjust the volume to 1 L to prepare a 150 μg / L fengzhisu aqueous solution.

[0074] (2) Select Longjing 31 and Longdao 20 rice seeds with full and uniform grain size, disinfect them with 75% alcohol by volume for 3 min, rinse them with distilled water for 3 times, and soak them in 150 μg / L Fengzhisu aqueous solution for 48 h. For the blank control, soak the seeds in deionized water.

[0075] (3) The soaked Longjing 31 and Longdao 20 rice seeds were used in a potted experiment. Specifically, the seeds with white heads after soaking were transplanted into a flower pot with an inner diameter of 25 cm and a height of 17.5 cm (each pot was filled with 4.5 kg of planting soil, and 0.74 g of ammonium sulfate, 1.47 g of urea, and 0.49 g of diammonium phosphate were added to the soil of each pot and mixed well). Fifteen seeds were sown in each pot. When the seedlings grew the second true leaf, the seedlings were thinned out, that is, one seedling with good and consistent growth was retained in each pot. Each group of treatments was set up with 25 parallels, and a representative pot of rice was selected for photographing and recording at each growth cycle (see Figure 2 The tillering number and yield of rice were counted at the tillering stage and maturity stage. The results are shown in Table 3.

[0076] Table 3 Statistical results of the effect of Fengzhisu aqueous solution seed soaking on the tillering number and yield of Longjing 31 and Longdao 20 rice varieties

[0077]

[0078]

[0079] As can be seen from Table 3, after soaking the seeds of different varieties of rice with Fengzhisu aqueous solution, the tiller number and yield of rice during growth were significantly improved. In the tillering stage, compared with the control, the tiller number of Longjing 31 increased by 24.14%, and the tiller number of Longdao 20 increased by 14.52%; in the maturity stage, compared with the control, the effective tiller number and yield of Longjing 31 increased by 24.44% and 28.14%, respectively, and the effective tiller number and yield of Longdao 20 increased by 25.57% and 30.79%, respectively.

[0080] Example 4: Application of Fengzhisu in increasing rice plant height, tillering number and yield by seed soaking in field experiments

[0081] The application described in this embodiment is to soak rice seeds in a 150 μg / L Fengzhisu aqueous solution at room temperature for 48 hours, and then directly sow them in the field for rice field experiments. The specific steps are as follows:

[0082] (1) Weigh 150 μg of fengzhisu, dissolve it in deionized water, and adjust the volume to 1 L to prepare a 150 μg / L fengzhisu aqueous solution.

[0083] (2) The Longjing 31 rice seeds with full and uniform grain size were selected and disinfected with 75% alcohol by volume for 3 min. After being rinsed with distilled water for 3 times, the seeds were soaked in a 150 μg / L Fengzhisu aqueous solution for 48 h. The seeds of the CK group were soaked in deionized water.

[0084] (3) The soaked Longjing 31 rice seeds were used in field experiments. Specifically, the seeds with white heads after soaking were sown manually by direct seeding (hole sowing) in an area of ​​80 m 2 , row spacing is 30cm, hole spacing is 30cm, sowing rate is 5-10 seeds per hole, and fertilizer rate is urea 150kg / hm 2 , Potassium oxide 70kg / hm 2 , Phosphorus pentoxide 70kg / hm 2 .

[0085] The plant height and tiller number of rice were counted at the seedling stage, tillering stage and heading stage. The results are shown in Table 4.

[0086] Table 4 Statistical results of the effect of Fengzhisu aqueous solution soaking on rice plant height and tiller number in the field

[0087]

[0088] It can be seen from Table 4 that after the rice seeds were soaked in an aqueous solution of 150 μg / L Fengzhisu and then directly seeded in the field, the growth of rice at different stages was significantly promoted, and the number of tillers was also significantly increased. Compared with the control, the rice plant height at the seedling stage increased by 15.58%; the rice plant height and the number of tillers at the tillering stage increased by 7.47% and 41.43% respectively; the rice plant height and the number of tillers at the heading stage increased by 2.52% and 28.57% respectively.

[0089] During the rice maturity period, the number of effective panicles per square meter, the number of panicles, the number of filled grains, the fruiting rate, the thousand-grain weight, and the yield per mu were counted. The results are shown in Table 5. Photographs were taken and recorded at each growth cycle of rice (seedling stage, tillering stage, heading stage, and maturity stage) (see Figure 3 ).

[0090] Table 5 Effect of seed soaking with Fengzhisu aqueous solution on rice yield in the field

[0091]

[0092] It can be seen from Table 5 that after the rice seeds were soaked in an aqueous solution of Fengzhisu at a concentration of 150μg / L and then directly seeded in the field, the rice yield was significantly improved. Compared with the control, the number of effective panicles per square meter, the number of grains per panicle, the number of filled grains, the thousand-grain weight and the yield per mu increased by 15.21%, 19.70%, 19.47%, 2.63% and 20.58% respectively.

[0093] Example 5: Application of Fengzhisu in the form of coating to promote corn / soybean seed germination at room temperature / low temperature in the laboratory

[0094] The application described in this embodiment is to coat corn seeds and soybean seeds with an effective concentration of 150 μg / L Fengzhisu aqueous solution, and then place the coated corn seeds and soybean seeds in a laboratory under normal temperature or low temperature stress environment for germination experiments. The specific steps are as follows:

[0095] (1) Weigh 1500 μg of fengzhisu, dissolve it in deionized water, and make up to 1 L to prepare a stock solution of 1500 μg / L.

[0096] (2) Take 100 μL of the mother solution in step (1), mix it with 900 μL of coating agent (4.23% metalaxyl·meconazole microemulsion of "Ding Miao Xin", containing 1.88% metalaxyl and 2.35% meconazole), mix it with 500 corn seeds or soybean seeds, dry it, and use 900 μL of coating agent (4.23% metalaxyl·meconazole microemulsion of "Ding Miao Xin", containing 1.88% metalaxyl and 2.35% meconazole) for blank control.

[0097] (3) The coated corn seeds and soybean seeds were transferred to a 9 cm diameter culture dish covered with sterilized filter paper, 15 corn seeds or soybean seeds were placed in each culture dish, 5 mL of sterilized deionized water was added to each culture dish, and the process was repeated 6 times. The culture dishes were placed in a dark environment at room temperature (28°C) for 4 days and in a dark environment at low temperature (15°C) for 7 days, and the germination of the corn seeds and soybean seeds was observed. The root length and sprout length of the corn seeds and soybean seeds were counted, and the results are shown in Table 6.

[0098] Table 6 Statistical results of the effect of Fengzhisu coating on the germination of corn and soybean seeds at room temperature and low temperature

[0099]

[0100]

[0101] As can be seen from Table 6, coating corn seeds and soybean seeds with Fengzhisu aqueous solution can improve the germination rate of corn and soybean seeds and enhance the cold resistance of seeds. Under normal temperature conditions, compared with the normal temperature control, the germination rate, sprout length and root length of corn after coating increased by 6.46%, 18.92% and 9.41% respectively, and the germination rate and root length of soybean after coating increased by 5.60% and 31.61% respectively; under low temperature conditions, compared with the low temperature control, the germination rate, sprout length and root length of corn after coating increased by 9.56%, 21.08% and 20.65% respectively, and the germination rate and root length of soybean after coating increased by 12.57% and 22.62% respectively.

[0102] Example 6: Application of Fengzhisu in coating mode to promote the development of corn / soybean seedlings in normal temperature / low temperature pot experiments

[0103] The application described in this embodiment is to coat corn seeds and soybean seeds with an effective concentration of 150 μg / L Fengzhisu aqueous solution, and then sow the coated corn seeds and soybean seeds in pots and place them in a laboratory under normal temperature or low temperature stress environment. The specific steps are as follows:

[0104] (1) Weigh 1500 μg of fengzhisu, dissolve it in deionized water, and make up to 1 L to prepare a stock solution of 1500 μg / L.

[0105] (2) Take 100 μL of the mother solution in step (1), mix it with 900 μL of coating agent (4.23% metalaxyl·meconazole microemulsion of "Ding Miao Xin", containing 1.88% metalaxyl and 2.35% meconazole), mix it with 500 corn seeds or soybean seeds, dry it, and use 900 μL of coating agent (4.23% metalaxyl·meconazole microemulsion of "Ding Miao Xin", containing 1.88% metalaxyl and 2.35% meconazole) for blank control.

[0106] (3) The coated corn seeds and soybean seeds were used in potted experiments. Specifically, the coated seeds were transplanted into flower pots with an inner diameter of 12 cm and a height of 10 cm. Each pot was filled with 200 g of planting soil and 5 seeds were planted in each pot. Each group of treatments was set up with 15 parallels. They were cultured at room temperature (28°C) and low temperature (15°C), respectively. The photoperiod was 12L / 12D. The room temperature culture lasted for 14 days and the low temperature culture lasted for 21 days. The plant height and root length of the corn seedlings and soybean seedlings were counted, and the results are shown in Table 7. Figure 4 and Figure 5 shown.

[0107] Table 7 Statistical results of the effects of Fengzhisu coating on corn and soybean seedlings at room temperature and low temperature

[0108]

[0109]

[0110] As can be seen from Table 7, coating corn seeds and soybean seeds with Fengzhisu aqueous solution can promote the development of corn seedlings and soybean seedlings, and enhance the cold resistance of corn and soybean. Under normal temperature conditions, compared with the normal temperature control, the plant height and root length of corn after coating increased by 30.29% and 16.90%, respectively, and the plant height and root length of soybean after coating increased by 16.23% and 25.14%, respectively; under low temperature conditions, compared with the low temperature control, the plant height and root length of corn after coating increased by 15.35% and 15.81%, respectively, and the plant height and root length of soybean after coating increased by 26.48% and 38.91%, respectively.

[0111] Example 7: Application of Fengzhisu spraying treatment in promoting the growth of cucumbers and tomatoes

[0112] The application described in this embodiment is to spray cucumber seedlings and tomato seedlings with a Fengzhisu aqueous solution with a concentration of 1500 μg / L, and the specific steps are as follows:

[0113] (1) Weigh 1500 μg of fengzhisu, dissolve it in deionized water, make the volume to 1 L, add 500 μL of Tween 20 to prepare a fengzhisu aqueous solution with a concentration of 1500 μg / L.

[0114] (2) Select cucumber and tomato seeds with full and uniform seeds, plant them in flower pots, and culture them in a constant temperature incubator at 24°C and a light intensity of 120-150m -2 s -1 , light / dark cycle 16h / 8h, relative humidity 60%. When cucumber and tomato grew to 2 leaves, each pot of seedlings was sprayed with 50mL of 1500μg / L Fengzhisu aqueous solution. After 21 days of cultivation, the plant height and root length of cucumber and tomato seedlings were counted, and the results are shown in Table 8.

[0115] Table 8 Statistical results of the effects of spraying Fengzhisu aqueous solution on potted cucumber seedlings and tomato seedlings

[0116]

[0117] It can be seen from Table 8 that the development of cucumber seedlings and tomato seedlings was significantly promoted after spraying with the Fengzhisu aqueous solution. The plant height and root length of cucumber seedlings increased by 26.32% and 20.00% respectively after spraying, and the plant height and root length of tomato seedlings increased by 10.04% and 15.70% respectively after spraying.

[0118] Example 8: Application of Fengzhisu spraying treatment in promoting the growth of Chinese cabbage, spinach and lettuce

[0119] The application described in this embodiment is to spray Chinese cabbage, spinach and lettuce with a Fengzhisu aqueous solution with a concentration of 1500 μg / L. The specific steps are as follows:

[0120] (1) Weigh 1500 μg of fengzhisu, dissolve it in deionized water, make the volume to 1 L, add 500 μL of Tween 20 to prepare a fengzhisu aqueous solution with a concentration of 1500 μg / L.

[0121] (2) Select Chinese cabbage, rapeseed, and lettuce seeds with full and uniform grain size, plant them in flower pots, and place them in a constant temperature incubator at 24°C and a light intensity of 120-150m -2 s -1, light / dark cycle 16h / 8h, relative humidity 60%. When Chinese cabbage, rapeseed, and lettuce grew to 2 leaves, each pot of seedlings was sprayed with 50mL of 1500μg / L Fengzhisu aqueous solution. After 21 days of continuous cultivation, the leaf areas of Chinese cabbage, spinach, and lettuce seedlings were counted, and the results are shown in Table 9.

[0122] Table 9 Statistical results of the effects of spraying Fengzhisu aqueous solution on potted Chinese cabbage, spinach and lettuce seedlings

[0123]

[0124] It can be seen from Table 9 that after the Chinese cabbage, spinach and lettuce seedlings were sprayed with the Fengzhisu aqueous solution, the development of the Chinese cabbage, spinach and lettuce seedlings was significantly promoted. The leaf areas of the Chinese cabbage, spinach and lettuce increased by 19.84%, 11.53% and 13.55% respectively after spraying.

[0125] Although the present invention has been disclosed as above in the form of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. Use of fengzhisu or its analogues as an active ingredient of a plant growth regulator in promoting rice seed germination, characterized in that: The application uses a 15-300 μg / L aqueous solution of Fengzhisu or an aqueous solution of Fengzhisu analogue 1 or an aqueous solution of Fengzhisu analogue 2 as a plant growth regulator to soak rice seeds; the Fengzhisu is 4-methoxybenzoic acid, the Fengzhisu analogue 1 is 2-methoxybenzoic acid, and the Fengzhisu analogue 2 is 3-methoxybenzoic acid.

2. The use of Fengzhisu or its analogues as an active ingredient of a plant growth regulator in improving the cold resistance of rice seeds, characterized in that: The application uses a 15-300 μg / L aqueous solution of Fengzhisu or an aqueous solution of Fengzhisu analogue 1 or an aqueous solution of Fengzhisu analogue 2 as a plant growth regulator to soak rice seeds to promote the germination of the rice seeds under low temperature conditions, wherein the low temperature is 15-18°C; the Fengzhisu is 4-methoxybenzoic acid, the Fengzhisu analogue 1 is 2-methoxybenzoic acid, and the Fengzhisu analogue 2 is 3-methoxybenzoic acid.

3. The use of Fengzhisu as an active ingredient of a plant growth regulator in promoting rice development, characterized in that: The invention discloses a method for soaking rice seeds with a 150 μg / L aqueous solution of Fengzhisu as a plant growth regulator, so as to promote the growth and tillering of rice at different growth stages; the Fengzhisu is 4-methoxybenzoic acid.

4. The application of Fengzhisu as an active ingredient of a plant growth regulator in increasing rice yield, characterized in that: The invention uses a 150 μg / L Fengzhisu aqueous solution as a plant growth regulator to soak rice seeds; the Fengzhisu is 4-methoxybenzoic acid.

5. The use of Fengzhisu as an active ingredient of a plant growth regulator in promoting the germination of grain crop seeds, characterized in that: The invention discloses a seed coating agent with a concentration of 150 μg / L of Fengzhisu as a plant growth regulator to coat the seeds of grain crops; the Fengzhisu is 4-methoxybenzoic acid.

6. The use of Fengzhisu as an effective ingredient of a plant growth regulator in improving the cold resistance of grain crop seeds, characterized in that: The invention discloses a seed coating agent with a concentration of 150 μg / L of Fengzhisu as a plant growth regulator to coat grain crop seeds, so as to promote the germination of grain crop seeds under low temperature conditions, wherein the low temperature is 15-18° C. The Fengzhisu is 4-methoxybenzoic acid.

7. The use of Fengzhisu as an active ingredient of a plant growth regulator in promoting the growth of seedlings of grain crops, characterized in that: The invention discloses a seed coating agent with a concentration of 150 μg / L of Fengzhisu as a plant growth regulator to coat the seeds of grain crops; the Fengzhisu is 4-methoxybenzoic acid.

8. Application of Fengzhisu as an active ingredient of plant growth regulator in improving the cold resistance of grain crop seedlings, characterized in that: The invention discloses a seed coating agent with a concentration of 150 μg / L of Fengzhisu as a plant growth regulator to coat grain crop seeds, so as to promote the growth of grain crop seedlings under low temperature conditions; the Fengzhisu is 4-methoxybenzoic acid.

9. The use of Fengzhisu as an active ingredient of a plant growth regulator in promoting the growth of fruit vegetable seedlings, characterized in that: The invention uses a 1500 μg / L aqueous solution of Fengzhisu as a plant growth regulator to spray vegetable seedlings; the Fengzhisu is 4-methoxybenzoic acid.

10. The use of Fengzhisu as an active ingredient of a plant growth regulator in promoting the growth of leafy vegetable seedlings, characterized in that: The invention uses a 1500 μg / L aqueous solution of Fengzhisu as a plant growth regulator to spray vegetable seedlings; the Fengzhisu is 4-methoxybenzoic acid.

Images