Use of forchlorfenuron or its analogues as plant growth regulators

Abstract

The application of fentanyl or its analogues as plant growth regulators belongs to the technical field of plant growth regulators. To screen plant growth regulators from microbial sources, this invention provides a plant growth regulator containing an aqueous solution of fentanyl or its analogues, with a concentration of fentanyl or its analogues ranging from 15 μg / L to 1500 μg / L. This invention studied the effects of fentanyl and its analogues on plant growth and development using seed germination experiments, pot experiments, and field experiments. It was found that fentanyl and its analogues can promote the germination of rice, corn, and soybean seeds, increase tillering and yield in rice, and promote the growth of cucumber, tomato, bok choy, spinach, and lettuce seedlings. As a novel, highly efficient, low-toxicity, and low-cost plant growth regulator, it has development and utilization value and application prospects in agricultural production.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulator technology, specifically relating to the application of cytosine or its analogues as plant growth regulators. Background Technology

[0002] Plant growth regulators play a crucial role in modern agriculture and horticulture, regulating plant growth and development processes to improve crop yield and quality. While traditional plant growth regulators are mostly prepared through chemical synthesis, the development of biotechnology has led to increased interest in microbial-derived plant growth regulators. Microorganisms possess rich diversity and metabolic pathways, enabling them to produce a variety of substances with plant growth-regulating activities. These substances offer advantages such as high efficiency, low toxicity, and environmental friendliness, providing new pathways for sustainable agricultural development.

[0003] Anisolic acid, also known as 4-methoxybenzoic acid, is an important organic synthesis intermediate and modifier, as well as a crucial raw material in preservatives and pharmaceuticals, widely used in the medical, food, and daily chemical industries. For example, anisolic acid can participate in the synthesis of methyl anisate and ethyl anisate, both important raw materials for flavoring, toothpaste, and dental cleaning agents. 2-Methoxybenzoic acid and 3-methoxybenzoic acid are analogs of anisolic acid. 2-Methoxybenzoic acid, also known as o-methoxybenzoic acid, can be used in organic synthesis, preservatives, fragrances, and pharmaceuticals; 3-methoxybenzoic acid, also known as m-methoxybenzoic acid, can be used as a pesticide and pharmaceutical intermediate. However, there are currently no reports on the use of anisolic acid or its analogs as plant growth regulators in existing technologies. Previous studies in our laboratory have found that anisolic acid can be isolated from the metabolites of endophytic fungi in *Ipomoea aquatica*, exhibiting low toxicity, with acute toxicity showing a subcutaneous LD50 in mice. 50 =400mg / kg, oral LD50 in rabbits 50 >5000mg / kg. Therefore, it would be of great significance to use cytokinin or its analogues as plant growth regulators. Summary of the Invention

[0004] To screen for plant growth regulators derived from microorganisms, this invention previously isolated a plant-promoting compound from the metabolites of endophytic fungi in *Ipomoea aquatica*, naming it *Fengzhisu*. Identification of *Fengzhisu* revealed it to be anisic acid (4-methoxybenzoic acid). Furthermore, this invention investigated the plant-promoting properties of *Fengzhisu* analogue 1 (2-methoxybenzoic acid) and *Fengzhisu* analogue 2 (3-methoxybenzoic acid), finding that both also possessed plant-promoting properties. Based on these research findings, this invention provides the application of *Fengzhisu* or its analogues as plant growth regulators, with the specific technical solution as follows:

[0005] The first objective of this invention is to provide the application of fentanyl or its analogues as an effective component of plant growth regulators in promoting rice seed germination. The application involves soaking rice seeds in an aqueous solution of fentanyl, fentanyl analogue 1, or fentanyl analogue 2 at a concentration of 15-300 μg / L. The fentanyl is 4-methoxybenzoic acid, fentanyl analogue 1 is 2-methoxybenzoic acid, and fentanyl analogue 2 is 3-methoxybenzoic acid.

[0006] In one embodiment of the present invention, the soaking is performed at room temperature for 48 hours.

[0007] In one embodiment of the present invention, promoting rice seed germination refers to increasing the germination rate, shoot length, and root length of rice seeds.

[0008] Preferably, the concentration of the aqueous solution of fenestration 1, or the aqueous solution of fenestration 2 is 150 μg / L.

[0009] The second objective of this invention is to provide the application of fentanyl or its analogues as effective components of plant growth regulators in improving the cold resistance of rice seeds. The application involves soaking rice seeds in an aqueous solution of fentanyl, fentanyl analogue 1, or fentanyl analogue 2 at a concentration of 15-300 μg / L to promote germination of rice seeds under low-temperature conditions, wherein the low temperature is 15-18°C. The fentanyl is 4-methoxybenzoic acid, fentanyl analogue 1 is 2-methoxybenzoic acid, and fentanyl analogue 2 is 3-methoxybenzoic acid.

[0010] In one embodiment of the present invention, the soaking is performed at room temperature for 48 hours.

[0011] In one embodiment of the present invention, promoting the germination of rice seeds under low temperature conditions refers to increasing the germination rate, shoot length, and root length of rice seeds under low temperature conditions.

[0012] Preferably, the concentration of the aqueous solution of fenestration 1, or the aqueous solution of fenestration 2 is 150 μg / L.

[0013] The third objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in promoting rice development. The application involves soaking rice seeds in an aqueous solution of 150 μg / L 4-methoxybenzoic acid to promote the growth and tillering of rice at different growth stages.

[0014] In one embodiment of the present invention, the soaking is performed at room temperature for 48 hours.

[0015] In one embodiment of the present invention, promoting the growth and tillering of rice at different growth stages refers to increasing the plant height of rice during the seedling stage, tillering stage, and heading stage, and increasing the number of tillers during the tillering stage, heading stage, and maturity stage.

[0016] The fourth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in improving rice yield. The application involves soaking rice seeds in an aqueous solution of 4-methoxybenzoic acid at a concentration of 150 μg / L as a plant growth regulator.

[0017] In one embodiment of the present invention, the soaking is performed at room temperature for 48 hours.

[0018] In one embodiment of the present invention, the increase in rice yield refers to increasing the number of effective panicles per square meter, the number of grains per panicle, the number of filled grains, the thousand-grain weight, and the yield per mu.

[0019] The fifth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in promoting the germination of grain crop seeds. The application uses a seed coating agent with a 4-methoxybenzoic acid concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds.

[0020] In one embodiment of the present invention, the food crops include corn and soybeans.

[0021] In one embodiment of the present invention, the seed coating agent is prepared by mixing a 1500 μg / L aqueous solution of Fengzhisu with a 4.23% metalaxyl-mancozeb microemulsion (containing 1.88% metalaxyl and 2.35% thiamethoxam) at a volume ratio of 1:9.

[0022] In one embodiment of the present invention, promoting the germination of grain crop seeds refers to increasing the germination rate, shoot length, and root length of grain crop seeds.

[0023] The sixth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in improving the cold resistance of grain crop seeds. The application uses a seed coating agent with a 4-methoxybenzoic acid concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds to promote germination of grain crop seeds under low temperature conditions, wherein the low temperature is 15-18℃; the 4-methoxybenzoic acid is 4-methoxybenzoic acid.

[0024] In one embodiment of the present invention, the food crops include corn and soybeans.

[0025] In one embodiment of the present invention, promoting the germination of grain crop seeds under low temperature conditions refers to increasing the germination rate, shoot length, and root length of grain crop seeds under low temperature conditions.

[0026] The seventh objective of this invention is to provide the application of the above-mentioned fengzhixin as an effective component of plant growth regulator in promoting the growth of grain crop seedlings, wherein the application uses a seed coating agent with a fengzhixin concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds; wherein fengzhixin is 4-methoxybenzoic acid.

[0027] In one embodiment of the present invention, the food crops include corn and soybeans.

[0028] In one embodiment of the present invention, promoting the growth of grain crop seedlings refers to increasing the plant height and root length of grain crop seedlings.

[0029] The eighth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in improving the cold resistance of grain crop seedlings. The application uses a seed coating agent with a 4-methoxybenzoic acid concentration of 150 μg / L as a plant growth regulator to coat grain crop seeds to promote the growth of grain crop seedlings under low temperature conditions.

[0030] In one embodiment of the present invention, the food crops include corn and soybeans.

[0031] In one embodiment of the present invention, promoting the growth of grain crop seedlings under low temperature conditions refers to increasing the plant height and root length of grain crops under low temperature conditions.

[0032] The ninth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in promoting the growth of fruit vegetable seedlings. The application involves spraying vegetable seedlings with an aqueous solution of 4-methoxybenzoic acid at a concentration of 1500 μg / L as a plant growth regulator.

[0033] In one embodiment of the present invention, the fruit vegetables include, but are not limited to, cucumbers and tomatoes.

[0034] In one embodiment of the present invention, promoting the growth of fruit vegetable seedlings refers to increasing the plant height and root length of fruit vegetable seedlings.

[0035] The tenth objective of this invention is to provide the application of 4-methoxybenzoic acid as an effective component of plant growth regulator in promoting the growth of leafy vegetable seedlings. The application involves spraying vegetable seedlings with an aqueous solution of 4-methoxybenzoic acid at a concentration of 1500 μg / L as a plant growth regulator.

[0036] In one embodiment of the present invention, the leafy vegetables include, but are not limited to, bok choy, spinach, and lettuce.

[0037] In one embodiment of the present invention, promoting the growth of leafy vegetable seedlings refers to increasing the leaf area of ​​leafy vegetable seedlings.

[0038] The beneficial effects of this invention are:

[0039] This invention discovers that aqueous solutions of 4-methoxybenzoic acid or its analogues (2-methoxybenzoic acid or 3-methoxybenzoic acid) can act as plant growth regulators to regulate the growth and development of grain crops, oil crops, and vegetables. The specific effects are as follows:

[0040] This invention, through seed germination experiments, found that soaking rice seeds in an aqueous solution of fentanyl or its analogues can promote germination of rice seeds under normal temperature and low temperature stress conditions, specifically by increasing the germination rate, shoot length, and root length of rice seeds. Similarly, coating corn or soybean seeds with a coating agent containing an aqueous solution of fentanyl can promote germination of corn or soybean seeds under normal temperature and low temperature stress conditions, specifically by increasing the germination rate, shoot length, and root length of rice seeds.

[0041] This invention, through indoor and outdoor pot experiments and field experiments, found that soaking rice seeds in an aqueous solution of fenzhixin can increase the plant height and tiller number of rice at different growth stages, as well as increase the number of effective panicles, grains per panicle, number of filled grains, thousand-grain weight, and yield per acre. Coating corn or soybean seeds with a coating agent containing an aqueous solution of fenzhixin can promote the growth of corn or soybean seedlings under normal temperature and low temperature stress conditions, specifically by increasing the plant height and root length of soybean or corn seedlings.

[0042] Furthermore, this invention also found that spraying vegetable seedlings with an aqueous solution of Fengzhisu can promote their growth, specifically by increasing the height and root length of cucumber or tomato seedlings, and increasing the leaf area of ​​bok choy, spinach, or lettuce seedlings.

[0043] In summary, the aqueous solution of fenestrant or its analogues provided by this invention promotes seed germination and plant growth, increases tillering or branching, and enhances plant yield and stress resistance. It can be used as a novel, highly efficient, low-toxicity, and low-cost plant growth regulator in agricultural production, demonstrating significant development and application potential. This invention provides a research foundation for its further registration, mass production, and large-scale agricultural use, and has important practical significance. Attached Figure Description

[0044] Figure 1 The effect of soaking seeds in a 150 μg / L solution of fenzhixin on the germination of rice seeds under low-temperature conditions in the laboratory is shown in the figure.

[0045] Figure 2The figure shows the effect of soaking seeds in a 150 μg / L solution of fenestrant on the growth status of different rice varieties at the tillering and maturity stages in an outdoor pot experiment; among them, Figure 2 In the diagram, A represents the growth status of Longjing 31 rice during the tillering stage. Figure 2 B in the diagram represents the growth status of Longdao 20 rice during the tillering stage. Figure 2 C in the diagram represents the growth status of Longjing 31 rice at maturity. Figure 2 D in the figure represents the growth status of Longdao 20 rice at maturity;

[0046] Figure 3 The figure shows the effect of soaking seeds in a 150 μg / L solution of fenzhixin on the growth status of Longjing 31 rice at different growth stages in a field experiment; among them, Figure 3 In the diagram, A represents the growth status of rice seedlings. Figure 3 B in the diagram represents the growth status of rice during the tillering stage. Figure 3 C in the diagram represents the growth status of rice during the heading stage. Figure 3 D in the diagram represents the growth status of mature rice.

[0047] Figure 4 The figure shows the effect of coating with 150 μg / L fenestrant aqueous solution on the growth status of maize in laboratory pot experiments at room temperature and low temperature; among which... Figure 4 In the diagram, A represents the growth status of corn after 14 days of incubation at room temperature. Figure 4 B in the figure represents the growth status of corn after 21 days of low-temperature culture;

[0048] Figure 5 The figure shows the effect of 150 μg / L fenestration agent aqueous solution coating on soybean growth in laboratory pot experiments at room temperature and low temperature; among them, Figure 5 In the diagram, A represents the growth status of soybeans after 14 days of incubation at room temperature. Figure 5 B in the figure represents the growth status of soybeans after 21 days of low-temperature cultivation. Detailed Implementation

[0049] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments and accompanying drawings. The embodiments described herein are not all embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0050] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, and the materials, reagents and instruments used are conventional materials, reagents and instruments in the art, which can be obtained by those skilled in the art through commercial channels.

[0051] The fenestration compound described in this invention is also known as anisolic acid or 4-methoxybenzoic acid, and its chemical structure is shown in Formula 1; the fenestration compound analog 1 described in this invention is also known as 2-methoxybenzoic acid, and its chemical structure is shown in Formula 2; the fenestration compound analog 2 described in this invention is also known as 3-methoxybenzoic acid, and its chemical structure is shown in Formula 3.

[0052]

[0053] Example 1: Application of Fengzhisu in promoting rice seed germination in the laboratory under ambient or low temperature conditions via seed soaking.

[0054] The application described in this embodiment involves soaking rice seeds in an aqueous solution of 15 μg / L to 300 μg / L of aphthylamine for 48 hours at room temperature. The soaked rice seeds are then placed in a laboratory under ambient temperature or low-temperature stress conditions for germination experiments. The specific steps are as follows:

[0055] (1) Weigh 300 μg of cypermethrin, dissolve it in deionized water, and make up to 1 L to prepare a stock solution of 300 μg / L.

[0056] (2) Take different volumes of the mother liquor from step (1), add deionized water according to the proportion, and dilute to prepare aqueous solutions of cypermethrin with different concentrations between 15 μg / L and 300 μg / L. The concentrations are shown in Table 1.

[0057] (3) Select plump and uniformly sized Longjing 31 rice seeds, disinfect them with 75% alcohol for 3 minutes, rinse them with distilled water 3 times, and soak them in Fengzhisu aqueous solution of different concentration gradients for 48 hours.

[0058] (4) After soaking, the rice seeds were transferred to 9cm diameter petri dishes lined with sterile filter paper. Ten rice seeds were placed in each dish, and 5mL of sterile deionized water was added to each dish. This process was repeated six times. The petri dishes were then incubated at room temperature (28℃) in the dark for 4 days and at low temperature (15℃) in the dark for 7 days. The germination of the rice seeds was observed, and the root length and shoot length were recorded. The results are shown in Table 1 and... Figure 1 As shown.

[0059] Table 1. Statistical results of the effects of different concentrations of fenestrant aqueous solution on rice seed germination at room temperature / low temperature.

[0060]

[0061]

[0062] Table 1 shows that soaking rice seeds in aqueous solutions of different concentrations of fentanyl-rich water can promote seed germination, increase germination rate, and improve cold resistance. In the room temperature germination experiment, a fentanyl-rich water concentration of 150 μg / L showed the best germination-promoting effect, with germination rate, root length, and shoot length increasing by 5.45%, 10.07%, and 11.00%, respectively, compared to the room temperature control. In the low-temperature stress germination experiment, a fentanyl-rich water concentration of 150 μg / L also showed the best germination-promoting effect, with germination rate, root length, and shoot length increasing by 6.18%, 20.23%, and 13.74%, respectively, compared to the low-temperature control.

[0063] Example 2: Application of fenzhijin analogues in promoting rice seed germination in the laboratory under ambient or low temperature conditions via seed soaking.

[0064] The application described in this embodiment involves soaking rice seeds in aqueous solutions of 150 μg / L of fentanyl analogue 1 and fentanyl analogue 2 at room temperature for 48 hours. The soaked rice seeds are then placed in a laboratory under ambient temperature or low-temperature stress conditions for germination experiments. The specific steps are as follows:

[0065] (1) Weigh 150 μg of fentanyl analog 1 and 150 μg of fentanyl analog 2, dissolve them in deionized water and bring the volume to 1 L to prepare aqueous solutions of fentanyl analog 1 with a concentration of 150 μg / L and fentanyl analog 2 with a concentration of 150 μg / L.

[0066] (2) Select plump and uniformly sized Longjing 31 rice seeds, disinfect them with 75% alcohol for 3 minutes, rinse them with distilled water 3 times, and then soak them in the aqueous solutions of the above-obtained Fengzhixin analog 1 and Fengzhixin analog 2 for 48 hours. The blank control is soaked in deionized water.

[0067] (3) After soaking, the rice seeds were transferred into 9cm diameter petri dishes lined with sterile filter paper. Ten rice seeds were placed in each petri dish, and 5mL of sterile deionized water was added to each dish. This process was repeated 6 times. The petri dishes were then placed in the dark at room temperature (28℃) for 4 days and in the dark at low temperature (15℃) for 7 days. The germination of the rice seeds was observed, and the root length and shoot length of the rice were recorded. The results are shown in Table 2.

[0068] Table 2. Statistical results of the effects of aqueous solutions of fentanyl analogue 1 and fentanyl analogue 2 on rice seed germination at room temperature / low temperature.

[0069]

[0070] As shown in Table 2, soaking rice seeds in an aqueous solution of fenzhinin analogue 1 not only promotes seed germination and increases the germination rate, but also enhances the cold resistance of seeds under low-temperature stress. Specifically, under normal temperature conditions, compared with the normal temperature control, the germination rate, root length, and shoot length of rice seeds treated with the aqueous solution of fenzhinin analogue 1 increased by 3.57%, 7.65%, and 7.10%, respectively; under low temperature conditions, compared with the low temperature control, the germination rate, root length, and shoot length of rice seeds treated with the aqueous solution of fenzhinin analogue 1 increased by 3.70%, 7.97%, and 8.53%, respectively. Soaking rice seeds in an aqueous solution of fenestration analog 2 not only promotes seed germination and increases germination rate, but also enhances seed cold resistance under low-temperature stress. Specifically, at room temperature, compared with the room temperature control, the germination rate, root length, and shoot length of rice seeds treated with the aqueous solution of fenestration analog 2 increased by 3.57%, 10.04%, and 9.69%, respectively; at low temperature, compared with the low-temperature control, the germination rate, root length, and shoot length of rice seeds treated with the aqueous solution of fenestration analog 2 increased by 5.56%, 12.65%, and 10.68%, respectively.

[0071] Example 3: Application of Fengzhisu in increasing the number of tillers and yield of rice in an outdoor pot experiment using seed soaking.

[0072] The application described in this embodiment involves soaking Longjing 31 and Longdao 20 rice seeds in a 150 μg / L aqueous solution of fenzhisu for 48 hours at room temperature. The soaked rice seeds are then sown in pots for an outdoor potted rice experiment. The specific steps are as follows:

[0073] (1) Weigh 150 μg of cypermethrin, dissolve it in deionized water, and make up to 1 L to prepare a 150 μg / L cypermethrin aqueous solution.

[0074] (2) Select plump and uniformly sized Longjing 31 and Longdao 20 rice seeds, disinfect them with 75% alcohol for 3 min, rinse them with distilled water 3 times, and soak them in a 150 μg / L solution of Fengzhisu for 48 h. The blank control is soaked in deionized water.

[0075] (3) Soaked Longjing 31 and Longdao 20 rice seeds were used in pot experiments. Specifically, seeds with white tips after soaking were transplanted into pots with an inner diameter of 25cm and a height of 17.5cm (each pot contained 4.5kg of planting soil, with 0.74g of ammonium sulfate, 1.47g of urea, and 0.49g of diammonium phosphate added to each pot and mixed well). 15 seeds were sown in each pot. When the seedlings grew their second true leaf, thinning was carried out, that is, one healthy and uniform seedling was retained in each pot. Each treatment group was set up with 25 replicates. A representative pot of rice was selected and photographed and recorded at each growth stage (see...). Figure 2 The number of tillers and yield of rice were counted during the tillering and maturity stages, and the results are shown in Table 3.

[0076] Table 3. Statistical results of the effects of seed soaking in Fengzhisu aqueous solution on tiller number and yield of Longjing 31 and Longdao 20 rice varieties.

[0077]

[0078]

[0079] Table 3 shows that soaking rice seeds in a solution of fenzhisu significantly increased the number of tillers and yield during the rice growth process. During the tillering stage, compared to the control, the number of tillers in Longjing 31 increased by 24.14%, and the number of tillers in Longdao 20 increased by 14.52%. At maturity, compared to the control, the number of effective tillers and yield of Longjing 31 increased by 24.44% and 28.14%, respectively, while the number of effective tillers and yield of Longdao 20 increased by 25.57% and 30.79%, respectively.

[0080] Example 4: Application of Fengzhisu in improving rice plant height, tiller number, and yield through seed soaking in field experiments.

[0081] The application described in this embodiment involves soaking rice seeds in a 150 μg / L aqueous solution of cypermethrin at room temperature for 48 hours, and then directly sowing them in the field for a rice field experiment. The specific steps are as follows:

[0082] (1) Weigh 150 μg of cypermethrin, dissolve it in deionized water, and make up to 1 L to prepare a 150 μg / L cypermethrin aqueous solution.

[0083] (2) Select plump and uniformly sized Longjing 31 rice seeds, disinfect them with 75% alcohol for 3 minutes, rinse them with distilled water 3 times, and soak them in a 150μg / L solution of Fengzhisu for 48 hours. The CK group was soaked in deionized water.

[0084] (3) The soaked Longjing 31 rice seeds were used in a field experiment. Specifically, the seeds with white tips after soaking were manually sown by direct sowing (sowing in holes) over a sowing area of ​​80m². 2 Row spacing 30cm, hole spacing 30cm, sowing rate 5-10 seeds per hole, fertilizer application rate 150kg / hm² of urea. 2 Potassium oxide 70 kg / hm 2 Phosphorus pentoxide 70 kg / hm 2 .

[0085] The plant height and number of tillers were counted at the seedling stage, tillering stage, and heading stage of rice. The results are shown in Table 4.

[0086] Table 4. Statistical results of the effects of seed soaking in Fengzhisu aqueous solution on plant height and tiller number of rice in the field.

[0087]

[0088] As shown in Table 4, soaking rice seeds in a 150 μg / L solution of fenzhisu before direct sowing significantly promoted the growth of rice at different stages and increased the number of tillers. Compared with the control, the plant height of rice increased by 15.58% in the seedling stage; the plant height and number of tillers increased by 7.47% and 41.43% in the tillering stage, respectively; and the plant height and number of tillers increased by 2.52% and 28.57% in the heading stage, respectively.

[0089] At the rice maturity stage, the number of effective panicles per square meter, number of grains per panicle, number of filled grains, seed setting rate, thousand-grain weight, and yield per mu were recorded. The results are shown in Table 5. Photographs were taken and recorded at each growth stage of rice (seedling stage, tillering stage, heading stage, and maturity stage) (see Table 5). Figure 3 ).

[0090] Table 5. Effects of seed soaking in fenestrant solution on rice yield in the field.

[0091]

[0092] As shown in Table 5, soaking rice seeds in a 150 μg / L solution of fenzhisu before direct sowing significantly increased rice yield. Compared with the control, the number of effective panicles per square meter, the number of grains per panicle, the number of filled grains, the thousand-grain weight, and the yield per mu increased by 15.21%, 19.70%, 19.47%, 2.63%, and 20.58%, respectively.

[0093] Example 5: Application of Fengzhixin in promoting corn / soybean seed germination in the laboratory at room temperature / low temperature via coating.

[0094] The application described in this embodiment involves coating corn and soybean seeds with a 150 μg / L effective concentration of fenestrant aqueous solution. The coated corn and soybean seeds were then subjected to germination experiments in a laboratory under ambient temperature or low-temperature stress conditions. The specific steps are as follows:

[0095] (1) Weigh 1500 μg of cypermethrin, dissolve it in deionized water, and make up to 1 L to prepare a stock solution of 1500 μg / L.

[0096] (2) Take 100 μL of the mother liquor from step (1) and mix it with 900 μL of coating agent ("Dingmiaoxin" 4.23% metalaxyl-mancozeb microemulsion, containing 1.88% metalaxyl and 2.35% thiamethoxam). After mixing, mix it with 500 corn or soybean seeds and dry them. Use 900 μL of coating agent ("Dingmiaoxin" 4.23% metalaxyl-mancozeb microemulsion, containing 1.88% metalaxyl and 2.35% thiamethoxam) to coat the blank control.

[0097] (3) The coated corn and soybean seeds were transferred into 9cm diameter petri dishes lined with sterile filter paper. 15 corn or soybean seeds were placed in each petri dish, and 5mL of sterile deionized water was added to each dish. This process was repeated 6 times. The petri dishes were then incubated in the dark at room temperature (28℃) for 4 days and in the dark at low temperature (15℃) for 7 days. The germination of the corn and soybean seeds was observed, and the root length and shoot length of the corn and soybean seeds were recorded. The results are shown in Table 6.

[0098] Table 6. Statistical results of the effects of fenestration coating on the germination of maize and soybean seeds at room temperature and low temperature.

[0099]

[0100]

[0101] Table 6 shows that coating corn and soybean seeds with a solution of fenzhisu (a type of styrax-based compound) can improve the germination rate of both seeds and enhance their cold resistance. Under normal temperature conditions, compared with the normal temperature control, the germination rate, shoot length, and root length of coated corn seeds increased by 6.46%, 18.92%, and 9.41%, respectively; while the germination rate and root length of coated soybean seeds increased by 5.60% and 31.61%, respectively. Under low temperature conditions, compared with the low temperature control, the germination rate, shoot length, and root length of coated corn seeds increased by 9.56%, 21.08%, and 20.65%, respectively; while the germination rate and root length of coated soybean seeds increased by 12.57% and 22.62%, respectively.

[0102] Example 6: Application of Fengzhisu (a type of herbicide) in promoting the development of maize / soybean seedlings in a pot experiment at room temperature / low temperature using a coating method.

[0103] The application described in this embodiment involves coating corn and soybean seeds with a 150 μg / L effective concentration of fenestrant aqueous solution. The coated corn and soybean seeds are then sown in pots and placed under laboratory conditions at room temperature or low temperature stress. The specific steps are as follows:

[0104] (1) Weigh 1500 μg of cypermethrin, dissolve it in deionized water, and make up to 1 L to prepare a stock solution of 1500 μg / L.

[0105] (2) Take 100 μL of the mother liquor from step (1) and mix it with 900 μL of coating agent ("Dingmiaoxin" 4.23% metalaxyl-mancozeb microemulsion, containing 1.88% metalaxyl and 2.35% thiamethoxam). After mixing, mix it with 500 corn or soybean seeds and dry them. Use 900 μL of coating agent ("Dingmiaoxin" 4.23% metalaxyl-mancozeb microemulsion, containing 1.88% metalaxyl and 2.35% thiamethoxam) to coat the blank control.

[0106] (3) Coated corn and soybean seeds were used in pot experiments. Specifically, the coated seeds were transplanted into pots with an inner diameter of 12 cm and a height of 10 cm. Each pot contained 200 g of planting soil and 5 seeds were planted in each pot. Each treatment group was set up with 15 replicates. The seeds were cultured at room temperature (28℃) and low temperature (15℃) respectively, with a photoperiod of 12 L / 12 D. The seedlings were cultured at room temperature for 14 days and at low temperature for 21 days. The plant height and root length of corn seedlings and soybean seedlings were recorded. The results are shown in Table 7. Figure 4 and Figure 5 As shown.

[0107] Table 7. Statistical results of the effects of cypermethrin coating on maize and soybean seedlings at room temperature and low temperature.

[0108]

[0109]

[0110] Table 7 shows that coating corn and soybean seeds with a solution of fenzhisu (a type of styrax-based compound) can promote the development of corn and soybean seedlings and enhance their cold resistance. Under normal temperature conditions, compared with the normal temperature control, the plant height and root length of coated corn increased by 30.29% and 16.90%, respectively, while those of coated soybeans increased by 16.23% and 25.14%, respectively. Under low temperature conditions, compared with the low temperature control, the plant height and root length of coated corn increased by 15.35% and 15.81%, respectively, while those of coated soybeans increased by 26.48% and 38.91%, respectively.

[0111] Example 7: Application of Fengzhisu spraying treatment in promoting the growth of cucumbers and tomatoes

[0112] The application described in this embodiment involves spraying cucumber and tomato seedlings with a 1500 μg / L aqueous solution of cypermethrin. The specific steps are as follows:

[0113] (1) Weigh 1500 μg of fenzhi sucrose, dissolve it in deionized water, bring the volume to 1 L, add 500 μL of Tween 20, and prepare an aqueous solution of fenzhi sucrose with a concentration of 1500 μg / L.

[0114] (2) Select plump, uniformly sized cucumber and tomato seeds, plant them in flowerpots, and place them in a constant temperature incubator at 24℃ with a light intensity of 120-150 nm. -2 s -1 The light / dark cycle was 16h / 8h, and the relative humidity was 60%. When the cucumber and tomato seedlings had two leaves, each seedling was sprayed with 50mL of a 1500μg / L solution of a growth regulator. After 21 days of cultivation, the plant height and root length of the cucumber and tomato seedlings were recorded. The results are shown in Table 8.

[0115] Table 8. Statistical results of the effects of spraying with Fengzhisu aqueous solution on potted cucumber and tomato seedlings.

[0116]

[0117] As shown in Table 8, the development of cucumber and tomato seedlings was significantly promoted after spraying with Fengzhisu aqueous solution. The plant height and root length of cucumber seedlings increased by 26.32% and 20.00% respectively after spraying, while the plant height and root length of tomato seedlings increased by 10.04% and 15.70% respectively after spraying.

[0118] Example 8: Application of Fengzhisu spraying treatment in promoting the growth of bok choy, spinach and lettuce

[0119] The application described in this example involves spraying bok choy, spinach, and lettuce with a 1500 μg / L aqueous solution of fenestrant. The specific steps are as follows:

[0120] (1) Weigh 1500 μg of fenzhi sucrose, dissolve it in deionized water, bring the volume to 1 L, add 500 μL of Tween 20, and prepare an aqueous solution of fenzhi sucrose with a concentration of 1500 μg / L.

[0121] (2) Select plump, uniformly sized seeds of bok choy, rapeseed, and lettuce, plant them in flowerpots, and place them in a constant temperature incubator at 24℃ and a light intensity of 120-150 nm. -2 s -1The light / dark cycle was 16h / 8h, and the relative humidity was 60%. When the bok choy, rapeseed, and lettuce had two leaves, each seedling was sprayed with 50mL of a 1500μg / L solution of a growth regulator. After 21 days of cultivation, the leaf area of ​​the bok choy, spinach, and lettuce seedlings was counted. The results are shown in Table 9.

[0122] Table 9. Statistical results of the effects of spraying Fengzhisu aqueous solution on potted bok choy, spinach and lettuce seedlings.

[0123]

[0124] As shown in Table 9, the development of bok choy, spinach and lettuce seedlings was significantly promoted after being sprayed with Fengzhisu aqueous solution. The leaf area of ​​bok choy, spinach and lettuce increased by 19.84%, 11.53% and 13.55% respectively after spraying.

[0125] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be defined by the claims.

Claims

1. Use of a brassinosteroid or an analogue thereof as an effective ingredient of a plant growth regulator in promoting seed germination of rice, characterized in that, The application uses a fuzhijin water solution or a fuzhijin analogue 1 water solution or a fuzhijin analogue 2 water solution with a concentration of 75-200 μg / L as a plant growth regulator to soak rice seeds; the fuzhijin is 4-methoxybenzoic acid, the fuzhijin analogue 1 is 2-methoxybenzoic acid, and the fuzhijin analogue 2 is 3-methoxybenzoic acid.

2. Use according to claim 1, characterized in that, The application uses a fuzhijin water solution or a fuzhijin analogue 1 water solution or a fuzhijin analogue 2 water solution with a concentration of 75-200 μg / L as a plant growth regulator to soak rice seeds, so as to promote the germination of rice seeds under low-temperature conditions, and the low temperature is 15-18 ℃.

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