Pesticide composition containing polyoxin and application thereof

Abstract

The invention belongs to the technical field of pesticide sterilization, and particularly discloses a polyoxin-containing pesticide composition, the pesticide composition comprises an active component I and an active component II, the active component I is polyoxin B, the active component II is any one of sedaxane or bixafen, and the active component I is polyoxin B, and the active component II is any one of sedaxane and bixafen. The mass ratio of the active component I to the active component II is (1: 48)-(50: 1). The pesticide composition disclosed by the invention has a synergistic effect on various pathogenic bacteria, can effectively reduce the use dosage of pesticides, and is safe to crops and other non-target organisms.

Description

[0001] This invention application is a divisional application with application number CN202311225752.3, application date September 22, 2023, and invention name “A pesticide composition containing polyoxin and its application”. Technical Field

[0002] The invention belongs to the technical field of pesticide sterilization, and specifically relates to a pesticide composition containing polyoxin and application thereof. Background Art

[0003] Polyoxin B, also known as polyoxin, is a metabolite produced by Streptomyces aureus. It is a peptide pyrimidine nucleoside agricultural antibiotic produced by modern bioengineering technology. It has systemic and therapeutic effects and high target biological selectivity. Its mechanism of action is to inhibit the synthesis of chitin in the fungal cell wall. Its structure is similar to the chitin synthase substrate uridine diphosphate glucosamine, and it is a competitive inhibitor. Because it is a safe pesticide with high efficiency, low toxicity and no environmental pollution, it is widely used in the prevention and treatment of many important diseases of grain crops, fruits and vegetables.

[0004] Sedaxane, ISO common name: sedaxane, CAS number: 874967-67-6, chemical name: N-[2-[1,1'-bicyclopropyl]-2-ylphenyl]-3-(difluoromethyl)-1-methyl-1H-pyrazole-carboxamide, its structure is divided into cis and trans structures, with the trans structure being the main one. Sedaxane is a succinate dehydrogenase inhibitor fungicide, which affects the mitochondrial respiratory electron transport system of pathogens by inhibiting the protein complex II (i.e. succinate dehydrogenase) on the mitochondrial respiratory electron transport chain of pathogens, hindering their energy metabolism, inhibiting the growth of pathogens and causing their death. It is suitable for crops such as cereals, soybeans, rapeseed, potatoes, and sugarcane. It can be used for seed treatment to prevent and control diseases such as powdery mildew, head smut, black spot, and gray mold.

[0005] Bixafen is a fungicide developed by Bayer, with the chemical name N-(3',4'-dichloro-5-fluoro[1,1'-biphenyl]-2-yl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide. Bixafen is a succinate dehydrogenase inhibitor, which works by inhibiting succinate dehydrogenase in mitochondrial respiration. It is mainly used in crops such as cotton, barley, sunflower, soybean, wheat, and rapeseed to prevent and control diseases caused by ascomycetes, basidiomycetes, and deuteromycetes. Bixafen is effective in preventing and treating diseases such as leaf blight and rust in cereal crops.

[0006] In agricultural production, the large-scale use of single pesticides will lead to the development of target fungal resistance, which will greatly shorten the service life of the pesticides, cause the dosage of pesticides to increase year by year, and increase the negative impact on the environment year by year, which is not conducive to the sustainable and healthy development of agriculture. Summary of the invention

[0007] In view of the above problems, the present invention provides a pesticide composition containing polyoxin and its preparation, which has good prevention and control effects on various plant diseases, can effectively reduce the dosage of pesticides used, reduce the cost of pesticides, is safe for crops, and reduces pesticide residues in agricultural products.

[0008] In order to achieve the above object, the present invention adopts the following technical scheme: a pesticide composition containing polyoxin B, the pesticide composition comprising active ingredient I and active ingredient II, the active ingredient I is polyoxin B, and the active ingredient II is any one of fluazifop or bixafen;

[0009] Furthermore, the active ingredient II is fluazifop, and the mass ratio of the active ingredient I to the active ingredient II is 1:30 to 10:1;

[0010] Furthermore, the mass ratio of the active ingredient I to the active ingredient II is 1:20 to 10:1;

[0011] Furthermore, the active ingredient II is bixafen, and the mass ratio of the active ingredient I to the active ingredient II is 1:30 to 10:1;

[0012] Furthermore, the mass ratio of the active ingredient I to the active ingredient II is 1:24 to 10:1;

[0013] Furthermore, the total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 1% to 90% of the total weight of the pesticide composition;

[0014] Furthermore, the total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient I and the active ingredient II accounts for 2% to 80% of the total weight of the pesticide composition;

[0015] Furthermore, the pesticide composition includes, in addition to the active ingredient, agriculturally permitted auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0016] Furthermore, the wetting agent is selected from a mixture of one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, sapodilla powder, silkworm feces or soapberry powder;

[0017] Further, the dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers;

[0018] Further, the emulsifier is selected from calcium alkylbenzene sulfonate, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl biphenyl ether disulfonic acid magnesium salt, triethanolamine salt, benzyl dimethylphenol polyoxyethyl ether, alkylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol formaldehyde resin polyoxyethyl ether, phenethylphenol polyoxyethyl polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether. One or more of the mixture;

[0019] Furthermore, the thickener is selected from a mixture of one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

[0020] Furthermore, the disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

[0021] Furthermore, the antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea;

[0022] Furthermore, the defoaming agent is selected from silicone oil, silicone compounds, C 10 ~C 20 Saturated fatty acids or C 8 ~C 10 A mixture of one or more fatty alcohol compounds;

[0023] Further, the solvent is selected from a mixture of one or more of N,N-dimethylformamide, cyclohexanone, butyl ether, xylene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methyl pyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;

[0024] Furthermore, the stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate, and triphenyl phosphate;

[0025] Further, the penetrant is selected from a mixture of one or more of penetrant JFC, penetrant T, azone or silicone;

[0026] Furthermore, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;

[0027] Furthermore, the filler is selected from a mixture of one or more of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

[0028] The above substances can be obtained commercially;

[0029] The pesticide composition of the present invention can be prepared into any formulation form permitted in agriculture;

[0030] Furthermore, the formulation is in the form of a solid formulation, a liquid formulation and / or a seed treatment formulation;

[0031] Furthermore, the solid preparation is a direct-use solid preparation, a dispersible solid preparation or a soluble solid preparation;

[0032] Furthermore, the solid preparation for direct use is a powder, granule, pellet, tablet or strip;

[0033] The dispersible solid preparation is a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule or a water-dispersible tablet;

[0034] The soluble solid preparation is a soluble powder, a soluble tablet or a soluble granule;

[0035] Furthermore, the liquid preparation is a solution preparation, a dispersed liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;

[0036] Furthermore, the solution preparation is a soluble solution, a soluble gel, an oil or a film-spreading oil;

[0037] The dispersed liquid preparation is an emulsifiable concentrate, a latex, a dispersible liquid or an ointment;

[0038] The emulsion preparation is a water emulsion, an oil emulsion, a microemulsion or a lipid preparation;

[0039] The suspension preparation is a suspension concentrate, a microcapsule suspension concentrate, an oil suspension concentrate or a dispersible oil suspension concentrate;

[0040] The multiphase preparation is a suspoemulsion, a microcapsule suspension-suspension concentrate, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0041] Furthermore, the seed treatment formulation includes a seed treatment solid formulation or a seed treatment liquid formulation;

[0042] Furthermore, the seed treatment solid preparation is a seed treatment dry powder or a seed treatment dispersible powder;

[0043] The seed treatment liquid preparation is a seed treatment liquid, a seed treatment emulsion and / or a seed treatment suspension;

[0044] Furthermore, the dosage form of the preparation is a solid preparation and / or a liquid preparation, the solid preparation is, and the liquid preparation is.

[0045] The invention also discloses the application of the above pesticide composition and / or its preparation in preventing and controlling plant diseases.

[0046] Furthermore, the plant is a cereal crop; the cereal crop is wheat, and the disease is wheat fusarium head blight and wheat powdery mildew.

[0047] Furthermore, the plant disease is caused by one or more of the following pathogens: Mastigomycotina, Zygomycotina, Ascomycotina, Basidiomycotina and / or Deuteromycotina fungi;

[0048] Furthermore, the pesticide composition and / or its preparation is applied at an effective dose to the pathogens to be controlled or to the medium in which they grow.

[0049] The beneficial effects of the present invention are as follows:

[0050] 1) The pesticide composition of the present invention has a synergistic effect on a variety of pathogens and can be used to prevent and treat a variety of plant diseases, thus expanding the scope of application;

[0051] 2) The pesticide composition of the present invention can effectively reduce the dosage of pesticides and reduce the pesticide residues in agricultural products;

[0052] 3) The pesticide composition of the present invention can slow down the development of drug resistance of pathogens and is safe for crops and other non-target organisms. DETAILED DESCRIPTION

[0053] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0054] Preparation Example

[0055] Preparation Example 1: 20% polyoxin B·fluazifop suspension (1:1)

[0056] Calculated by weight percentage, 10% polyoxin B, 10% fluazifop, 3% alkylphenol polyoxyethylene ether, 4% fatty alcohol polyoxyethylene ether phosphate, 1% polycarboxylic acid sodium salt, 1% magnesium aluminum silicate, 0.25% xanthan gum, 5% propylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water make up the balance.

[0057] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0058] Preparation Example 2: 24% polyoxin B·bixafen suspension (1:3)

[0059] Calculated by weight percentage, 6% polyoxin B, 18% bixafen, 1% isotridecyl alcohol polyoxyethylene ether, 2% naphthalenesulfonate formaldehyde condensate, 3% alkylphenol polyoxyethylene ether phosphate, 0.3% carboxyethyl cellulose, 2% magnesium aluminum silicate, 5% propylene glycol, 0.25% silicone oil, and deionized water make up the balance.

[0060] Preparation method: Same as Preparation Example 1.

[0061] Preparation Example 3: 30% polyoxin B·fluazide water dispersible granules (1:2)

[0062] By weight percentage, 10% polyoxin B, 20% fluazifop, 10% sodium lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 6% white carbon black, 25% starch and kaolin make up the balance.

[0063] Preparation method: according to the formula ratio, the active ingredient is added to the carrier, and the surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying and sieving; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0064] Preparation Example 4: 35% polyoxin B·bixafen water dispersible granules (1:6)

[0065] Calculated by weight percentage, 5% polyoxin B, 30% bixafen, 8% naphthalenesulfonate formaldehyde condensate, 6% sodium lignin sulfonate, 5% pulverized powder BX, 10% ammonium sulfate and kaolin make up the balance.

[0066] Preparation method: Same as Preparation Example 3.

[0067] Preparation Example 5: 30% polyoxin B·fluazide oxadiazine wettable powder (2:1)

[0068] Calculated by weight percentage, 20% polyoxin B, 10% fluazifop, 8% sodium lignin sulfonate, 7% alkyl naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 15% white carbon black, and kaolin make up the balance.

[0069] Preparation method: Active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, stirred evenly in a stirring kettle, and crushed and mixed evenly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0070] Preparation Example 6: 42% polyoxin B·bixafen wettable powder (1:5)

[0071] By weight percentage, 7% polyoxin B, 35% bixafen, 7% naphthalenesulfonate formaldehyde condensate, 8% sodium lignin sulfonate, 3% pulverized powder BX, and kaolin make up the balance.

[0072] Preparation method: Same as Preparation Example 5.

[0073] Indoor joint action test

[0074] Example 1: Indoor joint action test of different agents on wheat scab

[0075] Test basis: The test refers to NY / T 1156.2-2006 "Guidelines for Agricultural Indoor Bioassay Tests for Fungicides Part 2: Plate Method for Inhibition of Pathogen Mycelial Growth".

[0076] Test target: Fusarium graminearum.

[0077] Test instruments: wet heat sterilizer, clean workbench, constant temperature light incubator, electric drum windproof drying oven, 1 / 10,000 electronic balance, pipette, alcohol lamp, small beaker, volumetric flask, triangular flask, culture dish (Φ9cm), puncher, inoculator, ruler, etc.

[0078] The target culture conditions were as follows: Fusarium graminearum stored in a refrigerator at 4°C was transferred to potato dextrose agar medium, and cultured in a dark incubator at 26°C for 4 days to activate it and set aside.

[0079] Test agents: 32% polyoxin B technical, 92% bixafenac technical, 96% fluazifop technical.

[0080] Preparation of medicines: Dissolve and dilute the above medicines with appropriate solvents respectively, design different ratios according to the purpose of mixing and the activity of the medicines, and prepare the single dose and each group of mixed medicines into the required series of mass concentrations.

[0081] Experimental repetition: 4 culture dishes were set up for each concentration of the test agent, with a total of 4 repetitions, 1 culture dish for each repetition, and a treatment without the agent was set up as a blank control.

[0082] Drug treatment: Under aseptic operation conditions, add the pre-melted sterilized culture medium to the sterile conical flask according to the test treatment, and quantitatively draw the drug solution from low concentration to high concentration, add it to the above conical flask respectively, and shake it thoroughly. Then pour an equal amount into 4 culture dishes to make drug-containing plates of corresponding concentrations.

[0083] Inoculation: Cut the cultured pathogen from the edge of the colony with a sterile puncher of 5 mm in diameter under sterile conditions, and inoculate the cake into the center of the drug-containing plate with the mycelium side facing up. Cover the plate with a lid and place it in an incubator at a suitable temperature for culture.

[0084] Data investigation: Investigate the growth of pathogenic mycelium based on the growth of mycelium in the blank control culture dish. Use a ruler to measure the diameter of the colony in centimeters (cm). Measure the diameter of each colony once using the cross method, take the average value, and record the original data of all replicates for each treatment.

[0085] Data statistics and analysis: According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated. The unit is percentage (%) and the calculation result is rounded to two decimal places.

[0086] D=D 1 -D 2

[0087] Where:

[0088] D——colony growth diameter;

[0089] D 1 ——colony diameter;

[0090] D 2 ——Diameter of mushroom cake.

[0091]

[0092] Where:

[0093] I——hyphae growth inhibition rate;

[0094] D 0 ——Blank control colony growth diameter;

[0095] D T ——The growth diameter of the colony after treatment with chemicals.

[0096] The data were processed by probability value analysis. The DPS statistical analysis system was used to calculate the toxicity regression line and EC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0097] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0098] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0099]

[0100] Where:

[0101] ATI——Actual Toxicity Index of Mixture;

[0102] S——EC of standard medicine 50 , the unit is milligrams per liter (mg / L);

[0103] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0104] TTI=TI A ×P A +TI B ×P B

[0105] Where:

[0106] TTI – Theoretical Toxicity Index of Mixtures;

[0107] TI A ——A: toxicity index of the agent;

[0108] P A ——The percentage of agent A in the mixture, in percentage (%);

[0109] TI B ——Toxicity index of agent B;

[0110] P B ——The percentage of agent B in the mixture, in percentage (%).

[0111]

[0112] Where:

[0113] CTC – Co-toxicity coefficient;

[0114] ATI——Actual Toxicity Index of Mixture;

[0115] TTI - Theoretical Toxicity Index of Mixture.

[0116] The indoor test results are shown in the following table:

[0117] Table 1 Results of the indoor combined action test of polyoxin B and bixafen against wheat fusarium head blight

[0118] Test reagents and ratios Regression equation (Y=) <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient Polymycin B y=5.2243+1.4808x 0.7056 - Bixafen y=4.3903+1.2430x 3.0940 - Polyoxin B: bixafen = 1:50 y=4.5740+1.0863x 2.4670 117.610 Polyoxin B: bixafen = 1:30 y=4.6160+1.1643x 2.1372 130.518 Polyoxin B: bixafen = 1:10 y=4.7864+1.0706x 1.5832 149.441 Polyoxin B: bixafen = 1:5 y=4.9114+1.2495x 1.1774 168.003 Polyoxin B: bixafen = 1:3 y=5.0899+1.2341x 0.8445 198.443 Polyoxin B: bixafen = 1:1 y=5.1640+1.1224x 0.7143 160.876 Polyoxin B: bixafen = 3:1 y=5.2358+1.1142x 0.6142 142.353 Polyoxin B: bixafen = 5:1 y=5.2504+1.1184x 0.5972 135.597 Polyoxin B: bixafen = 10:1 y=5.2240+0.9920x 0.5945 127.646 Polyoxin B: bixafen = 30:1 y=5.2226+1.1255x 0.6341 114.118 Polyoxin B: bixafen = 50:1 y=5.1992+1.0880x 0.6560 109.214

[0119] Table 2 Results of the indoor combined effect test of polyoxin B and fluazifop-butyl on wheat fusarium head blight

[0120]

[0121]

[0122] The indoor test results in Tables 1 and 2 show that compounding polyoxin with either bixafen or fluazifop has a significant synergistic effect on wheat fusarium head blight within a reasonable range.

[0123] Example 2: Indoor joint action test of different treatment agents against wheat powdery mildew

[0124] Test basis: The test refers to NY / T 1156.4-2006 "Guidelines for Agricultural Indoor Bioassay Tests for Fungicides Part 4: Pot Method for Control of Wheat Powdery Mildew".

[0125] Test target: Wheat powdery mildew (Blumeria graminis f.sp.tritici).

[0126] Test agent: Select the wheat variety Luyuan 502 susceptible to powdery mildew and plant them in pots. When the seedlings grow to the 2- to 3-leaf stage, number them and set them aside.

[0127] Experimental agents: 32% polyoxin B technical, 92% bixafen technical, 96% fluazifop technical, all of which were provided by the R&D center of Hailier Pharmaceutical Group.

[0128] Test preparation: Dissolve and dilute the above agents with appropriate solvents respectively, design different ratios according to the purpose of mixing and the activity of the agents, and prepare the single agent and each group of mixed agents into the required series of mass concentrations.

[0129] Inoculation: Shake off the fresh spores of powdery mildew produced on the leaves of diseased wheat within 24 hours and inoculate them evenly on the potted wheat seedlings at the 2-3 leaf stage. Each treatment has 4 pots and 10 plants in each pot.

[0130] Chemical treatment: Spray the chemical evenly on the spare wheat seedlings by spraying and let them dry naturally. The experiment sets up a treatment without chemical as a blank control. After applying the chemical, place it under appropriate conditions for cultivation.

[0131] Data investigation: According to the incidence of blank control, the investigation is graded. The following grading method is used:

[0132] Level 0: no lesions;

[0133] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0134] Level 3: The lesion area accounts for 6-15% of the entire leaf area;

[0135] Level 5: The lesion area accounts for 16-25% of the entire leaf area;

[0136] Level 7: The lesion area accounts for 26-50% of the entire leaf area;

[0137] Level 9: The lesion area accounts for more than 50% of the entire leaf area.

[0138] Data statistics and analysis:

[0139] The disease index is calculated according to formula (1)

[0140]

[0141] Where:

[0142] X—disease index;

[0143] N i —Number of diseased leaves at each level;

[0144] i—relative level value;

[0145] N—Total number of leaves surveyed.

[0146] The control effect is calculated according to formula (2)

[0147]

[0148] Where:

[0149] P——control effect, in %;

[0150] CK——blank control disease index;

[0151] PT - drug treatment index.

[0152] Referring to the standard method of bioassay NY / T 1156.6 - 2006, the synergistic effect of the mixed agents was evaluated according to the co - toxicity coefficient method (CTC) of Sun Yunpei, that is, CTC ≤ 80 is antagonistic effect, 80 < CTC < 120 is additive effect, and CTC ≥ 120 is synergistic effect. The co - toxicity coefficient (CTC) was calculated according to formulas (3), (4), and (5).

[0153]

[0154] In the formula:

[0155] ATI—the measured toxicity index of the mixture;

[0156] S—the EC of the standard agent 50 , with the unit of milligram per liter (mg / L);

[0157] M—the EC of the mixture 50 , with the unit of milligram per liter (mg / L).

[0158] TTI = TI A ×P A +TI B ×P B

[0159] In the formula:

[0160] TTI—the theoretical toxicity index of the mixture;

[0161] TI A —the toxicity index of agent A;

[0162] P A —the percentage content of agent A in the mixture, with the unit of percentage (%);

[0163] TI B —the toxicity index of agent B;

[0164] P B —the percentage content of agent B in the mixture, with the unit of percentage (%).

[0165]

[0166] In the formula:

[0167] CTC—the co - toxicity coefficient;

[0168] ATI—the measured toxicity index of the mixture;

[0169] TTI—the theoretical toxicity index of the mixture.

[0170] The DPS data processing software was used to calculate the test results and the toxicity regression equations, EC 50 , and calculate the co-toxicity coefficient (CTC) of different ratios of the two drugs to screen out the best ratio of the test drugs. Record the original data of all replicates of each treatment.

[0171] The indoor test results are shown in the following table:

[0172] Table 3 Results of the indoor combined effect test of polyoxin B and bixafen against wheat powdery mildew

[0173] Test reagents and ratios Regression equation (Y=) <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient Polymycin B y=4.6070+1.1420x 2.2087 - Bixafen y=4.0234+1.0634x 8.2866 - Polyoxin B: bixafen = 1:48 y=4.2821+0.8620x 6.6469 118.040 Polyoxin B: bixafen = 1:24 y=4.3391+0.8880x 5.5501 134.501 Polyoxin B: bixafen = 1:12 y=4.2858+0.9870x 4.2913 159.368 Polyoxin B: bixafen = 1:6 y=4.3153+1.3731x 3.1869 186.647 Polyoxin B: bixafen = 1:2 y=4.5805+1.1719x 2.2923 188.548 Polyoxin B: bixafen = 1:1 y=4.6899+1.0325x 1.9969 174.659 Polyoxin B: bixafen = 2:1 y=4.8027+0.8251x 1.7341 168.586 Polyoxin B: bixafen = 5:1 y=4.7224+0.9831x 1.9156 131.358 Polyoxin B: bixafen = 10:1 y=4.7511+0.9393x 1.8409 128.551 Polyoxin B: bixafen = 30:1 y=4.6974+1.0182x 1.9821 114.133 Polyoxin B: bixafen = 50:1 y=4.6590+1.0851x 2.0617 108.693

[0174] Table 4 Results of the indoor combined effect test of polyoxin B and fluazifop-butyl on wheat powdery mildew

[0175] Test reagents and ratios Regression equation (Y=) <![CDATA[EC 50 (mg / L)]]> Co-toxicity coefficient Polymycin B y=4.6241+1.1057x 2.1877 - Cyclohexanil y=4.4865+1.0562x 3.0632 - Polyoxin B: oxazolidinone = 1:40 y=4.6690+0.7712x 2.6865 112.920 Polyoxin B: oxazolidinone = 1:20 y=4.6743+0.8774x 2.3508 127.868 Polyoxin B: oxazolidinone = 1:10 y=4.7146+0.8651x 2.1376 138.270 Polyoxin B: oxazolidinone = 1:5 y=4.7453+0.9467x 1.8582 154.540 Polyoxin B: oxazolidinone = 1:2 y=4.8532+0.8148x 1.5140 178.512 Polyoxin B: oxazolidinone = 1:1 y=4.8621+1.0521x 1.3524 188.736 Polyoxin B: oxazolidinone = 2:1 y=4.8305+0.8316x 1.5990 151.224 Polyoxin B: oxazolidinone = 5:1 y=4.7515+1.1063x 1.6773 136.954 Polyoxin B: oxazolidinone = 10:1 y=4.7707+0.8908x 1.8088 124.174 Polyoxin B: oxazolidinone = 20:1 y=4.7354+0.9554x 1.8920 117.224 Polyoxin B: oxazolidinone = 40:1 y=4.6832+0.9580x 2.1412 102.889

[0176] The indoor test results in Tables 3 and 4 show that compounding polyoxin with either bixafen or fluazifop has a significant synergistic effect on wheat powdery mildew within a reasonable range.

[0177] Field efficacy test

[0178] Example 3: Field efficacy test of different pesticides on wheat scab

[0179] Test basis: The test refers to NY / T 1464.15-2007 "Guidelines for Field Efficacy Tests of Pesticides Part 15: Fungicides for Control of Wheat Fusarium Head Blight".

[0180] Test target: Wheat fusarium graminearum.

[0181] Experimental crops: wheat (Ningmai No. 13).

[0182] Test location: The test was carried out in a wheat field in Shangyu District, Shaoxing City, Zhejiang Province. The soil fertility of the test site was relatively high. Wheat was sown on November 12, 2019, with a sowing rate of 18 kg per mu.

[0183] Experimental agents: Relevant experimental agents and dosages are shown in the table below.

[0184] Plot arrangement: The plot treatments of test agent, control agent and blank control were arranged randomly.

[0185] Plot area and repetition: Plot area 20m 2 , each treatment was repeated 4 times.

[0186] Time and frequency of application: The first application of pesticide is carried out at the early stage of wheat flowering. Use Gongnong-16 backpack sprayer for conventional spraying, and strive to spray evenly during spraying.

[0187] Experimental investigation: The control effect was investigated during the milky stage of wheat. Samples were taken at 5 points on the diagonal of each plot, 100 ears were investigated at each point, and the areas of dead ears were graded according to the percentage of the area of ​​the whole ear. The number of diseased ears at each level and the total number of ears were recorded.

[0188] Grading method:

[0189] Level 0: The whole ear is disease-free;

[0190] Level 1: The dead ear area accounts for less than 1 / 4 of the whole ear area;

[0191] Level 3: The dead ear area accounts for 1 / 4-1 / 2 of the whole ear area;

[0192] Level 5: The dead ear area accounts for 1 / 2-3 / 4 of the whole ear area;

[0193] Level 7: The area of ​​dead ears accounts for more than 3 / 4 of the whole ear area.

[0194] Calculation method of drug efficacy:

[0195]

[0196] Safety investigation: After application, irregular observations were conducted. No yellowing, spots, deformities or other phytotoxicity phenomena were observed on the wheat stems and leaves in any of the treatment agents. The wheat grew normally, indicating that the pesticide composition of the present invention is safe for wheat within the range of the application dosage and application frequency of this test.

[0197] The results of the field efficacy test are shown in Table 5:

[0198] Table 5 Results of field efficacy tests of different pesticides on wheat fusarium head blight

[0199] deal with <![CDATA[Active ingredient dosage (g / hm 2 )]]> Diseased ear rate Disease index Control effect (%) 24% polyoxin B·bixafen suspension (1:3) 12 13.80 2.03 89.32 42% polyoxin B·bixafen WP (1:5) 12 20.55 3.06 83.86 20% polyoxin B·fluazifop SC (1:1) 8 16.45 2.54 86.64 30% Polyoxin B·Cyclomefone water dispersible granules (1:2) 8 13.50 2.11 88.86 20% cyfluthrin suspension 10 22.75 4.54 76.11 20% polyoxin B wettable powder 8 22.20 4.60 75.77 30% Bixafen suspension 30 24.50 5.47 71.18 Blank control - 62.95 18.99 /

[0200] It can be seen from the field efficacy test in Table 5 that a reasonable combination of polyoxin B and bixafen or pentocyclam can effectively enhance the control effect on wheat scab. The diseased ear rate of wheat in the treatment group of the composite preparation of the present invention is reduced, and the control effect is above 83%.

[0201] Example 4: Field efficacy test of different treatment agents against wheat powdery mildew

[0202] Test basis: The test was conducted with reference to GB / T 17980.22-2000 "Guidelines for field efficacy tests of pesticides (I) Fungicides for the control of powdery mildew of cereals".

[0203] Test object: Wheat powdery mildew (Blumeria graminis f.sp.tritici).

[0204] Experimental crop: wheat, variety is Shannong No. 22.

[0205] The test site was a wheat field in Cizhang Village, Tai'an City, Shandong Province. The previous crop was corn. The soil fertility of the test site was medium. The cultivation conditions (variety, soil type and fertilization) of the test plots were uniform and consistent, which was in line with local scientific agricultural practices.

[0206] Test drug: The test drug, control drug and dosage of active ingredient are shown in the table below.

[0207] Experimental plot arrangement: The experimental drug, control drug and blank control plots were arranged in random blocks, with each plot area of ​​20m 2 , each treatment was repeated 4 times.

[0208] Application method: According to the experimental design, the pesticide was applied at the early stage of powdery mildew. A manual sprayer was used for conventional spraying. The pesticide was applied twice, with an interval of 7 days between each application.

[0209] Survey method: Sampling is carried out at five fixed diagonal points in each plot, with each point surveying 0.25m 2 For wheat plants, after heading, the flag leaf and the first leaf under the flag leaf of each plant were investigated and graded according to the following grading method.

[0210] Grading Standard:

[0211] Level 0: no disease;

[0212] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0213] Level 3: The lesion area accounts for 6% to 15% of the entire leaf area;

[0214] Level 5: The lesion area accounts for 16% to 25% of the entire leaf area;

[0215] Level 7: The lesion area accounts for 26% to 50% of the entire leaf area;

[0216] Level 9: The lesion area accounts for more than 50% of the entire leaf area;

[0217] Investigation time: Before the first spraying, investigate and record the disease index. The efficacy investigation shall be conducted 7 days after the first application of the drug and 10 days after the last application of the drug.

[0218] Calculation method of drug efficacy:

[0219] The efficacy is calculated according to the following formula:

[0220]

[0221] The results of the field efficacy test are shown in Table 6:

[0222] Table 6 Results of field efficacy tests of different treatment agents against wheat powdery mildew

[0223]

[0224] The test results in Table 6 show that the preparation examples of 35% polyoxin B·bifenazolin water dispersible granules (1:6) and 20% polyoxin B·fluazifop suspension (1:1) have good control effects on wheat powdery mildew and are safe for wheat crops.

Claims

1. A pesticide composition containing polyoxin, characterized in that: The pesticide composition comprises an active ingredient I and an active ingredient II, wherein the active ingredient I is polyoxin B and the active ingredient II is fluazifop.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient I to the active ingredient II is 1:48 to 50:

1.

3. The pesticide composition according to claim 1, characterized in that The active ingredient II is fluazifop, and the mass ratio of the active ingredient I to the active ingredient II is 1:30 to 10:

1.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of the active ingredient I to the active ingredient II is 1:20 to 10:

1.

5. The pesticide composition according to claim 1, characterized in that The total weight of the pesticide composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 1% to 90%, preferably 2% to 80%, of the total weight of the pesticide composition.

6. The pesticide composition according to claim 1, characterized in that In addition to the active ingredients, the pesticide composition also includes agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into any formulation form permitted in agriculture, and the formulation form is a solid formulation or a liquid formulation; The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules; The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions; Preferably, the dosage form of the preparation is a solid preparation and / or a liquid preparation, the solid preparation is a water dispersible granule or a wettable powder, and the liquid preparation is a suspension.

8. Use of the pesticide composition and / or its preparation according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plants are cereal crops, and the diseases are wheat fusarium head blight and wheat powdery mildew.

10. The use according to claim 8, characterized in that: The pesticide composition and / or its preparation is applied at an effective dose to the pathogens to be controlled or to the medium where they grow.

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