Bactericidal composition for preventing and treating fungal diseases of tomatoes and application of bactericidal composition

Abstract

The invention relates to the technical field of pesticides, in particular to a bactericidal composition for preventing and treating fungal diseases of tomatoes and application. The active components of the bactericidal composition provided by the invention are trifluoropyridylamine and iminoctadine trioctyl alkyl benzene sulfonate. The bactericidal composition and the pesticide preparation containing the bactericidal composition provided by the invention not only have a good control effect on tomato gray leaf spots, but also have a better control effect on tomato gray mold. The synergistic composition improves the disease control effect of chemical agents, realizes multi-prevention by spraying at one time, can effectively reduce the dosage of the chemical agents, improves the control efficiency, and achieves the purposes of pesticide reduction and synergism and yield and income increase of vegetable farmers.

Description

Technical Field

[0001] The invention relates to the technical field of pesticides, and in particular to a bactericidal composition for preventing and controlling tomato fungal diseases and an application thereof. Background Art

[0002] The widespread planting of greenhouse tomatoes ensures the year-round supply of tomatoes, which is of great significance to enriching people's tables and ensuring the safety of the "vegetable basket". However, due to the high humidity and suitable temperature in the facility, it is very conducive to the occurrence and development of diseases. The occurrence of diseases such as gray mold and gray leaf spot seriously affects the yield and quality of greenhouse tomatoes.

[0003] Tomato gray leaf spot is caused by Stolonifera ( Stemphylium spp.) is an important disease caused by the fungus. The disease mainly harms the leaves. Usually, the lower and middle leaves of the plant are the first to be affected. In the early stage of the disease, the surface of the leaves is covered with dark green round or nearly round spots, which gradually expand to the surrounding areas. In the later stage, they are restricted by the veins and become polygonal. The middle part gradually fades to grayish white or grayish brown. The lesions are slightly sunken and are easy to break and perforate and fall off in the later stage. In severe cases, the lesions cover the entire leaf. Some lesions are connected into pieces and are irregular in shape, causing the leaves to dry up and fall off, and even causing the entire branch to turn yellow and dry up. In severe cases, the disease can also damage the petiole, stem, and even fruit, causing yield losses.

[0004] Tomato gray mold is caused by Botrytis cinerea ( Botrytis cinerea ) is a worldwide disease caused by infection. Gray mold can infect all above-ground parts of tomato plants, mainly fruits and leaves. Fruit disease often starts from the remaining petals and stigma, causing flower rot, and then spreads to the fruit surface and fruit stalk, usually first showing symptoms near the fruit pedicle, fruit stalk or navel. Before the fruit matures, the skin of the diseased part is grayish white, water-soaked and soft, and soon develops into irregular large spots. The fruit becomes rigid or moist and soft after losing water. Diseased fruits generally do not fall off. After the disease occurs, they come into contact with each other and spread. In severe cases, the entire bunch of fruits will rot, and a large number of conidia will be produced at the diseased part. The disease of leaves often starts from the tip of the leaf and expands inward in an inverted "V" shape. The lesions are initially water-soaked with irregular edges, and then light brown to yellow-brown with alternating dark and light ring patterns. Infected petals, stamens, etc. fall on the leaf surface, forming round or spindle-shaped lesions. Generally, the stem is less affected. After the disease occurs, the damaged part begins to appear water-stained, and then expands into long oval or long strip-shaped spots. When the disease spots surround the stem, the upper branches and leaves wilt and die. When it is humid, a gray-brown mold layer (commonly known as "mouse skin") grows densely on the affected fruit, leaves, and stems, and gradually black sclerotia of different sizes are scattered in the mold layer. Gray mold generally causes a yield loss of 10% to 20%, and in severe cases it can reach more than 60%, causing significant economic losses.

[0005] Gray mold and gray leaf spot are important diseases in greenhouse tomato production. In production, the prevention and control of these two diseases mainly rely on spraying chemical fungicides. However, high-efficiency and low-toxic fungicides usually have a single site of action and a high risk of resistance. How to delay the development of pathogen resistance, extend the service life of high-efficiency fungicides, and achieve multiple preventions with one spray can not only reduce prevention and control costs, improve prevention and control efficiency, but also reduce the amount of chemical agents used. This is a hot topic and difficulty in current research.

[0006] In view of this, the present invention is proposed. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a fungicide composition for preventing and controlling tomato fungal diseases and its application.

[0008] Specifically, the technical solution of the present invention is as follows: In a first aspect, the present invention provides a fungicide composition, the active components of which are trifluopyridine and biguanide tri-octylbenzene sulfonate, and the mass ratio of trifluopyridine to biguanide tri-octylbenzene sulfonate is 1:(1-5).

[0009] Preferably, the mass ratio of trifluopyridine amine to biguanide trioctylbenzene sulfonate is 1:1.

[0010] In a second aspect, the present invention provides a pesticide preparation, which contains the fungicide composition, and the fungicide composition accounts for 1%-20% of the pesticide preparation.

[0011] Preferably, the pesticide preparation is in the form of a nano water suspension.

[0012] Preferably, the pesticide formulation comprises the following components: 2-4 parts by mass of trifluopyridine, 2-4 parts by mass of biguanide trioctylbenzene sulfonate, 40-60 parts by volume of dichloromethane, 2-4 parts by mass of cellulose, 2-4 parts by mass of an emulsifying dispersant, and 150-250 parts by volume of water; wherein, when the unit of the mass part is g, the unit of the volume part is mL.

[0013] Further preferably, the emulsifying dispersant is sodium dodecyl sulfate and Nongru 600 in a mass ratio of 1:1-3.

[0014] Preferably, the pesticide formulation further comprises an adjuvant component, and the adjuvant component comprises: 0.5-1.5 parts by mass of ethylene glycol and 1-3 parts by mass of nano silicon dioxide.

[0015] Further preferably, the pesticide formulation comprises the following components: 3 parts by mass of trifluralin, 3 parts by mass of biguanide trioctylbenzene sulfonate, 50 parts by volume of dichloromethane, 3 parts by mass of cellulose, 3 parts by mass of emulsifying dispersant, 200 parts by volume of water, 1 part by mass of ethylene glycol, and 2 parts by mass of nano-silicon dioxide; wherein, when the unit of the mass part is g, the unit of the volume part is mL.

[0016] In a third aspect, the present invention provides a method for preparing the pesticide formulation, comprising: mixing trifluopyramide, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, removing dichloromethane after emulsification to obtain a nanopesticide mother liquor; and adding an auxiliary component to the nanopesticide mother liquor.

[0017] In a fourth aspect, the present invention provides the use of the fungicide composition or the pesticide preparation or the pesticide preparation prepared by the preparation method described in the claim in preventing and controlling tomato gray leaf spot and gray mold.

[0018] In a fifth aspect, the present invention provides a method for using the bactericidal composition or the pesticide preparation or the pesticide preparation prepared by the preparation method, controlling the application amount of the active component to 157.5-300 g / hectare, the application interval to 10±3 days, and the number of applications to 3±1 times.

[0019] Beneficial effects: The present invention provides a fungicide composition, the active components of which are trifluopyramide and biguanide trioctylbenzene sulfonate. The fungicide composition and the pesticide preparation containing the same provided by the present invention not only have a good control effect on tomato gray leaf spot, but also have a good control effect on tomato gray mold. The synergistic combination improves the control effect of chemical agents on diseases, achieves multiple control with one spray, can effectively reduce the amount of chemical agents used, improves the control efficiency, and achieves the purpose of reducing the amount of pesticides and increasing their efficiency, and increasing the production and income of vegetable farmers. DETAILED DESCRIPTION

[0020] On the one hand, the present invention provides a composite synergistic fungicidal composition for preventing and controlling tomato fungal diseases. According to the indoor joint toxicity test, the fungicidal composition of the present invention has good antibacterial activity against both Solanum solani and Botrytis cinerea, and the synergistic effect is significant. At the same time, according to the field efficacy test, the fungicidal composition of the present invention has good preventive effect on both tomato gray leaf spot and gray mold, achieving one spray for multiple preventions, reducing the number of times of application, effectively reducing the amount of chemical agents used, improving the prevention and control efficiency, and achieving the goal of reducing the amount of chemical pesticides and increasing their effectiveness.

[0021] The bactericidal composition of the present invention comprises trifluopyridine and biguanide trioctylbenzene sulfonate as active components.

[0022] Triflupyridam is a new generation of succinate dehydrogenase inhibitor (SDHI) fungicide developed and launched by Syngenta. It is another fungicide / nematicide after Bayer's fluopyram, and the second compound with nicotinamide structure (or pyridineamide structure) after BASF's boscalid. This agent not only has nematicidal activity, but also has good prevention and control effects on diseases such as wheat stem rot and ergot caused by Fusarium.

[0023] Biguanide trioctylbenzene sulfonate is a broad-spectrum fungicide with strong local permeability. It is a contact and preventive fungicide that mainly acts on the synthesis of lipid compounds and cell membrane function of pathogens, inhibits spore germination, germ tube elongation, and the formation of attached spores and hyphae. At present, it has been registered in my country for the prevention and control of asparagus stem blight, watermelon vine blight, grape and tomato gray mold, and cucumber powdery mildew.

[0024] Through a large number of studies, our team found that trifluopyramide and biguanide tri-octylbenzene sulfonate have good antibacterial activity against tomato gray leaf spot pathogen. In order to delay the resistance of pathogens to trifluopyramide, our team conducted a study on the combined toxicity of trifluopyramide and biguanide tri-octylbenzene sulfonate in different proportions against tomato gray leaf spot pathogen. During the research process, the present invention unexpectedly found that after trifluopyramide and biguanide tri-octylbenzene sulfonate were compounded, they not only had a toxicity enhancement effect on tomato gray leaf spot pathogen, but also showed good prevention effect in the field and had a good prevention and control effect on tomato gray mold, realizing one spray for multiple preventions, improving prevention and control efficiency, and achieving the effect of reducing the use of chemical pesticides and increasing their effectiveness.

[0025] In order to further improve the combined toxicity of the bactericidal composition, the present invention also studies the specific compounding method of the active components, which is as follows: In the active component, the mass ratio of trifluopyridine to biguanide trioctylbenzene sulfonate is 1:(1-5). By controlling the ratio between the two, the combined toxicity of the composition can be significantly improved while controlling the cost.

[0026] Further preferably, the mass ratio of trifluopyramide to biguanide trioctylbenzene sulfonate is 1:1, which can significantly improve the combined toxicity of the composition to tomato gray leaf spot and gray mold while controlling the cost.

[0027] The second aspect of the present invention provides a pesticide preparation containing the above-mentioned fungicide composition.

[0028] Specifically, the dosage form of the pesticide preparation is a nano water suspension.

[0029] The pesticide preparation of the present invention not only has a good control effect on tomato gray leaf spot, but also has a good control effect on tomato gray mold, realizing multiple control effects with one spray, improving the control efficiency, and achieving the purpose of increasing production and income for vegetable farmers.

[0030] The active component accounts for 1%-20% of the total mass of the pesticide preparation, which can be adjusted according to actual needs.

[0031] The pesticide preparation is in the form of a nanometer water suspension, and can also be made into a wettable powder, emulsifiable concentrate or the like as required.

[0032] The third aspect of the present invention provides a method for preparing the pesticide formulation, comprising: mixing trifluopyramide, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, removing dichloromethane after emulsification to obtain a nanopesticide mother liquid; and adding an auxiliary component to the nanopesticide mother liquid.

[0033] In one of the more specific and preferred embodiments, the preparation method of the pesticide preparation as a suspension comprises the following steps: dissolving 3 g of trifluoropyridamole and 3 g of biguanide trioctylbenzene sulfonate in 50 mL of organic solvent dichloromethane, adding 3 g of cellulose to obtain an oil phase, dissolving 3 g of an emulsifying dispersant (1 g of sodium dodecyl sulfonate and 2 g of Nongru 600) in 200 ml of water to obtain an aqueous phase, mixing the oil phase and the aqueous phase, and emulsifying them for 20 min using an ultrasonic pulverizer to obtain a primary emulsion. The primary emulsion is homogenized at high pressure for 15 min using a high-pressure homogenizer to obtain a fine emulsion. The fine emulsion is stirred in an electric stirrer for 24 h (speed 1200 rpm) to volatilize the organic solvent to obtain a nano pesticide mother liquor. Adding an auxiliary agent 1 g of ethylene glycol and 2 g of nano silicon dioxide to the nano pesticide mother liquor, grinding with 95 zirconium beads with a diameter of 0.3-0.4 mm, and finally obtaining a nano pesticide water suspension.

[0034] The fourth aspect of the present invention provides the use of the bactericidal composition or the pesticide preparation in preventing and controlling gray mold of tomato gray leaf spot. Experiments have shown that the implementation of the bactericidal composition or the pesticide preparation of the present invention can significantly improve the field prevention and control effect, achieve one spray for multiple preventions, significantly reduce the amount of chemical agents used, and achieve the purpose of increasing production and income for vegetable farmers.

[0035] The fifth aspect of the present invention provides a method for using the bactericidal composition or the pesticide preparation or the pesticide preparation prepared by the preparation method, wherein the application amount of the active component is controlled to be 157.5-300 g / hectare, the application interval is 10±3 days, and the number of applications is 3±1 times. The method of use provided by the present invention can maximize its medicinal activity and will not cause obvious pesticide damage to farmland and crops.

[0036] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The endpoints and any values ​​of the ranges disclosed in this specification are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0038] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "specific implementations", or "some specific implementations" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0039] In the examples provided in this specification, if no specific techniques or conditions are specified, the techniques or conditions described in the literature in this field or the product instructions are used. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased through regular channels.

[0040] In the following examples: trifluopyramide suspension, active ingredient concentration 450 g / L, purchased from the market. Biguanide tri-octyl benzene sulfonate wettable powder, active ingredient concentration 40%, purchased from the market. Biguanide tri-octyl benzene sulfonate, active ingredient concentration 90%, purchased from the market.

[0041] Example 1 This embodiment provides a nanopesticide preparation, which is specifically a 30 g / L trifluopyramide-biguanide tri-octylbenzene sulfonate nanosuspension (1:1).

[0042] This embodiment provides a method for preparing the above-mentioned nanopesticide preparation: 3 g of trifluoropyridamole and 3 g of biguanide trioctylbenzene sulfonate are dissolved in 50 mL of organic solvent dichloromethane, 3 g of cellulose is added to obtain an oil phase, 3 g of an emulsifying dispersant (1 g of sodium dodecyl sulfonate and 2 g of Nongru 600) is dissolved in 200 ml of water to obtain an aqueous phase, and after the oil phase and the aqueous phase are mixed, an ultrasonic pulverizer is used for emulsification for 20 min to obtain a primary emulsion. The primary emulsion is homogenized at high pressure for 15 min using a high-pressure homogenizer to obtain a fine emulsion. The fine emulsion is stirred in an electric stirrer for 24 h (speed 1200 rpm) to volatilize the organic solvent to obtain a nanopesticide mother liquor. Add 1 g of ethylene glycol and 2 g of nano-silicon dioxide as an auxiliary agent to the above-mentioned nanopesticide mother liquor, grind with 95 zirconium beads with a diameter of 0.3-0.4 mm, and finally obtain a nanopesticide water suspension.

[0043] Effect verification In order to further verify that the bactericidal composition obtained by the present invention has a good bactericidal effect, the following is explained through test examples. Due to space limitations, the present invention only records convincing formulas and tests.

[0044] In the following test examples: trifluopyramide suspension, active ingredient concentration 450 g / L, purchased from the market. Biguanide tri-octylbenzene sulfonate wettable powder, active ingredient concentration 40%, purchased from the market. Biguanide tri-octylbenzene sulfonate, active ingredient concentration 90%, purchased from the market. The compound test agents involved in the following test examples were all prepared from the formulas in the above examples.

[0045] Test Example 1 This test example is used to illustrate the combined toxicity of trifluopyramide and biguanide trioctylbenzene sulfonate against pathogens that cause major tomato diseases.

[0046] The mycelial growth rate method was used to determine the combined toxicity of trifluopyramide and biguanide trioctylbenzene sulfonate against tomato gray leaf spot and gray mold.

[0047] The tested pathogenic fungi strains: gray leaf spot pathogen (HSR3) and Botrytis cinerea (BDD2) were preserved by the Fungicide Research Laboratory, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences.

[0048] The test strain was pre-cultured using PDA medium, wherein the PDA medium contained 200 g of potato, 20 g of glucose, 10 g of agar, and 1000 mL of distilled water.

[0049] The original drug to be tested was dissolved in acetone to prepare a mother solution with a concentration of 10,000 mg / L for standby use. The preparation was directly dissolved in water to support a mother solution with a concentration of 10,000 mg / L. Triflupyridine and biguanide trioctylbenzene sulfonate were mixed at different mass ratios (1:1, 1:3 and 1:5) to obtain mixed solutions with different ratios. Then, a series of dilutions were made with sterile water and mixed with the culture medium at a ratio of 1:9 to prepare drug-containing PDA plates with a series of concentrations. A 5 mm diameter bacterial cake was taken from the edge of the pre-cultured strain and attached to the center of the drug-containing plate with the front side facing down. It was cultured at a constant temperature of 24 °C. When the control colony expanded to about 80% of the culture dish, the diameter of each treatment and the control was measured by the cross method. EC was calculated using dps software 50 and correlation coefficient.

[0050] Calculation of inhibition rate: Inhibition rate (%) = [(control colony growth - treatment colony growth germination rate) / control colony growth] × 100%.

[0051] Refer to the Wadley formula to calculate the synergistic coefficient after mixing the drugs: SR=EC 50 (th) / EC 50 (ob) In the above formula, a and b represent the proportion of the two fungicides in the mixture; EC 50 (A) and EC 50 (B) represent the actual observed EC of A and B respectively 50 Value, EC 50 (th) represents the theoretical EC of the mixture of two fungicides A and B in the ratio of a:b 50 Value, EC 50 (ob) is the actual observed EC after the two fungicides A and B are mixed in the ratio of a:b 50 SR>1.5, synergistic effect; SR<0.5, antagonistic effect; SR between 0.5 and 1.5 indicates additive effect.

[0052] The test results are shown in the following table: As can be seen from Table 1, the mixture of trifluopyramide and biguanide tri-octylbenzene sulfonate showed a good inhibitory effect on the mycelial growth of tomato gray leaf spot pathogen. The synergistic coefficients of each ratio (1:1-1:5) were all greater than 1.5, showing a toxicity enhancement effect. When the ratio of the two was 1:1, the synergistic coefficient was the largest, which was 3.0.

[0053] As can be seen from Table 2, trifluopyramide and biguanide trioctylbenzene sulfonate showed good inhibitory effect on the mycelial growth of tomato gray mold after mixing. When the ratio of the two was 1:1 and 1:5, the synergistic coefficient was greater than 1.5, showing a toxicity enhancement effect. When the ratio of the two was 1:3, the synergistic coefficient was 1.2, showing an additive toxicity effect.

[0054] Test Example 2 This test example is used to illustrate the field control effect of trifluopyramide and biguanide trioctylbenzene sulfonate on tomato gray leaf spot.

[0055] The test was conducted in accordance with the "Guidelines for Field Efficacy Tests of Pesticides" GB / T17980.111-2004 "Guidelines for Field Efficacy Tests of Fungicides for Controlling Tomato Leaf Mold" of the Institute for Drug Control, Ministry of Agriculture and Rural Affairs.

[0056] The experimental field is located in Xihe Village, Liuchu Town, Raoyang County, Hebei Province. The tomato variety is Rolla. The first spraying began before the occurrence of tomato gray leaf spot. The drug was used three times in succession, with an interval of about 10 days between each use. The disease situation in the field was investigated 10 days after the last use of the drug, and the disease index and prevention and control effect were calculated.

[0057] The test shows that 450 g / L triflupyridam suspension and 40% biguanide trioctylbenzene sulfonate wettable powder mixed in a mass ratio of 1:1 showed good control effect on tomato gray leaf spot. Compared with its chemical single agent, the amount of chemical agent used was significantly reduced, and the amount of chemical agent used was reduced by 20%, and there was no adverse effect on crops and other beneficial organisms at the test dose.

[0058] The nano-preparation 30g / L trifluopyridam·biguanide tri-octylbenzene sulfonate suspension concentrate sprayed at 350 mL / mu had a control effect of 86.89% on tomato gray leaf spot, which was not significantly different from the control effect of single-dose 450 g / L trifluopyridam suspension concentrate 30 mL / mu and 40% biguanide tri-octylbenzene sulfonate wettable powder 50 g / mu on tomato gray leaf spot, but the dosage was reduced by 22.2%; the nano-preparation 30g / L trifluopyridam·biguanide tri-octylbenzene sulfonate suspension concentrate sprayed at 450 mL / mu had a control effect of 90.09% on tomato gray leaf spot, which was significantly higher than the control effect of single-dose 450 g / L trifluopyridam suspension concentrate 30 mL / mu and 40% biguanide tri-octylbenzene sulfonate wettable powder 50 g / mu on tomato gray leaf spot; all test agents had no adverse effects on crops and other beneficial organisms at the test doses.

[0059] Test Example 3 This test example is used to illustrate the field control effect of trifluopyramide with other agents and their combined preparations on tomato gray mold.

[0060] The test was conducted in accordance with the "Guidelines for Field Efficacy Tests of Pesticides" GB / T17980.28-2000 "Guidelines for Efficacy Tests of Fungicides for Controlling Vegetable Botrytis Cinerea" of the Institute for Drug Control, Ministry of Agriculture and Rural Affairs.

[0061] The experimental field is located in Baitapu Village, Xushui District, Baoding City, Hebei Province. In the season when tomato gray mold often occurs, the first spray was carried out before the onset of gray mold. The drug was used three times in succession with an interval of about 7 days between each use. The disease situation in the field was investigated 9 days after the last use of the drug, and the disease index and prevention and control effect were calculated.

[0062] The test shows that 450 g / L triflupyridamole suspension and 40% biguanide trioctylbenzene sulfonate wettable powder mixed in a mass ratio of 1:1 showed good control effect on tomato gray mold. Compared with its chemical single agent, under similar control effect, the amount of chemical agent used was significantly reduced, and the amount of chemical agent used was reduced by 20%, and there was no adverse effect on crops and other beneficial organisms at the test dose.

[0063] The control effect of the nano-preparation 30g / L trifluopyridam·biguanide tri-octylbenzene sulfonate suspension at 350 mL / mu on tomato gray mold was 85.74%, which was not significantly different from the control effect of its single-dose 450 g / L trifluopyridam suspension at 30 mL / mu and 40% biguanide tri-octylbenzene sulfonate wettable powder at 50 g / mu on tomato gray mold, but the dosage was reduced by 22.2%; the control effect of the nano-preparation 30g / L trifluopyridam·biguanide tri-octylbenzene sulfonate suspension at 450 mL / mu on tomato gray mold was 89.30%, which was significantly higher than the control effect of its single-dose 450 g / L trifluopyridam suspension at 30 mL / mu and 40% biguanide tri-octylbenzene sulfonate wettable powder at 50 g / mu on tomato gray mold; The control effect of two treatment doses of 50% boscalid water dispersible granules (48 g / mu) on tomato gray mold was significantly higher than that of the conventional agent 50% boscalid water dispersible granules (48 g / mu). All the test agents had no adverse effects on crops and other beneficial organisms at the test doses.

[0064] Based on the above experiments, it can be seen that the combination of trifluopyramide and bisguanidine tri-octylbenzene sulfonate, or the preparation of the nano pesticide 30g / L trifluopyramide·bisguanidine tri-octylbenzene sulfonate suspension, can simultaneously prevent and control multiple diseases such as tomato gray leaf spot and gray mold, achieving multiple preventions with one spray, effectively reducing the use of chemical agents, improving prevention and control efficiency, and achieving the goal of increasing production and income for vegetable farmers.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bactericidal composition, characterized in that The active components are trifluoropyridamole and biguanide tri-octylbenzene sulfonate, and the mass ratio of trifluoropyridamole to biguanide tri-octylbenzene sulfonate is 1:(1-5).

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of trifluopyridine amine to biguanide trioctylbenzene sulfonate is 1:

1.

3. A pesticide formulation, characterized in that It contains the fungicide composition according to claim 1 or 2, and the fungicide composition accounts for 1%-20% of the pesticide preparation.

4. The pesticide preparation according to claim 3, characterized in that: The dosage form of the pesticide preparation is a nano water suspension.

5. The pesticide formulation according to claim 4, characterized in that: The pesticide preparation comprises the following components: 2-4 parts by mass of trifluopyridine, 2-4 parts by mass of biguanide trioctylbenzene sulfonate, 40-60 parts by volume of dichloromethane, 2-4 parts by mass of cellulose, 2-4 parts by mass of an emulsifying dispersant, and 150-250 parts by volume of water; wherein, when the unit of the mass part is g, the unit of the volume part is mL; Preferably, the emulsifying dispersant is sodium dodecyl sulfate and Nongru 600 in a mass ratio of 1:1-3.

6. The pesticide formulation according to claim 5, characterized in that: The pesticide preparation also includes an auxiliary component, which includes: 0.5-1.5 parts by weight of ethylene glycol and 1-3 parts by weight of nano silicon dioxide.

7. The pesticide preparation according to claim 6, characterized in that: The pesticide preparation comprises the following components: 3 parts by mass of trifluopyridine, 3 parts by mass of biguanide trioctylbenzene sulfonate, 50 parts by volume of dichloromethane, 3 parts by mass of cellulose, 3 parts by mass of an emulsifying dispersant, 200 parts by volume of water, 1 part by mass of ethylene glycol, and 2 parts by mass of nano silicon dioxide; wherein, when the unit of the mass part is g, the unit of the volume part is mL.

8. The method for preparing the pesticide formulation according to any one of claims 3 to 7, characterized in that: include: The invention discloses a method for preparing a nanopesticide mother liquid by mixing trifluopyridine, biguanide trioctylbenzene sulfonate, dichloromethane and cellulose to obtain an oil phase; mixing an emulsifying dispersant and water to obtain an aqueous phase; mixing the oil phase and the aqueous phase, removing dichloromethane after emulsification, and obtaining a nanopesticide mother liquid; and adding an auxiliary component to the nanopesticide mother liquid.

9. Use of the fungicidal composition according to claim 1 or 2, the pesticide preparation according to any one of claims 3 to 7, or the pesticide preparation prepared by the preparation method according to claim 8 in preventing and controlling tomato gray leaf spot and gray mold.

10. A method for using the fungicidal composition according to claim 1 or 2, the pesticide preparation according to any one of claims 3 to 7, or the pesticide preparation prepared by the preparation method according to claim 8, characterized in that: The application amount of the active component is controlled to be 157.5-300 g / hectare, the application interval is 10±3 days, and the number of applications is 3±1 times.

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