Special-effect prevention and treatment agent for rice sheath blight disease and preparation method thereof

Abstract

The invention relates to the field of pesticide preparations, and discloses a special-effect prevention and treatment agent for rice sheath blight disease and a preparation method thereof.The special-effect prevention and treatment agent for rice sheath blight disease is prepared from, by weight, 10-14 parts of thifluzamide, 15-19 parts of validamycin, 8-12 parts of bacillus subtilis powder, 3-5 parts of azadirachtin, 2-4 parts of chitinase and 1-3 parts of beta-1, 3, 4-triazole-1-one. 100 parts of the cleaning agent comprise 1-3 parts of 1, 3-glucanase, 3-5 parts of sodium lignin sulfonate, 2-3 parts of modified polyether organosilicone, 1.5-2 parts of sodium carboxymethyl cellulose, 2-4 parts of lauryl sodium sulfate, 3-5 parts of sodium alginate oligosaccharide, 1-2 parts of nanoscale zinc oxide and the balance of kaolin. Through fusion of thifluzamide and validamycin, starting from different action mechanisms of interfering germ energy metabolism, inhibiting cell wall synthesis and the like, precise striking is performed on rhizoctonia solani, the instant sterilization effect is improved, and meanwhile, through collocation with biological enzymes such as chitinase and beta-1, 3-glucanase, key components of germ cell walls are decomposed in a targeted manner.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide preparations, in particular to a special-effect control agent for rice sheath blight and a preparation method thereof. Background Art

[0002] Rice sheath blight is a fungal disease caused by Rhizoctonia solani, one of the three major rice diseases. Rice sheath blight has a great impact on yield, generally causing a 10%-30% reduction in rice yield, and up to more than 50% in severe cases. This is because the disease affects rice photosynthesis, nutrient absorption and transportation, resulting in a lower fruit set rate and a decrease in 1000-grain weight. In addition to yield losses, sheath blight also affects the quality of rice. The diseased rice grains are not full and have increased chalkiness, which affects both processing quality and eating quality.

[0003] Traditional prevention and control methods mainly rely on a few chemical agents, such as Jinggangmycin, which has been widely used for a long time. However, as the resistance of pathogens gradually increases, its prevention and control effect has been greatly reduced. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a special control agent for rice sheath blight and a preparation method thereof, which solves the problem that the control effect of traditional rice sheath blight control agents is greatly reduced as the drug resistance of the pathogen gradually increases.

[0005] To achieve the above objectives, the present invention is implemented through the following technical scheme: a special control agent for rice sheath blight, comprising the following raw materials in parts by weight: 10-14 parts of thiophanate-methyl, 15-19 parts of validamycin, 8-12 parts of Bacillus subtilis powder, 3-5 parts of azadirachtin, 2-4 parts of chitinase, 1-3 parts of β-1,3-glucanase, 3-5 parts of sodium lignin sulfonate, 2-3 parts of modified polyether silicone, 1.5-2 parts of sodium carboxymethyl cellulose, 2-4 parts of sodium dodecyl sulfate, 3-5 parts of sodium alginate oligosaccharides, 1-2 parts of nano-grade zinc oxide, and kaolin supplemented to 100 parts.

[0006] Preferably, the effective live bacteria count of the Bacillus subtilis powder is not less than 10 billion / g.

[0007] Preferably, the size of the nano-sized zinc oxide is in the range of 1-100 nm.

[0008] Preferably, the activity of the chitinase is greater than 50 U / mg, and the activity of the β-1,3-glucanase is greater than 20 U / mg.

[0009] A method for preparing a special-effect control agent for rice sheath blight comprises the following steps:

[0010] S1, first mixing: adding thiofuran, validamycin, sodium lignin sulfonate, sodium carboxymethyl cellulose and kaolin into a stirring mixer in order by weight and stirring to obtain a mixed powder;

[0011] S2, adding bacterial powder: then pre-treating the Bacillus subtilis bacterial powder by weight, and then adding it into the mixed powder by a peristaltic pump or a dropping device, stirring it, so that it is evenly dispersed in the powder to obtain a mixture;

[0012] S3, second mixing: then dissolving azadirachtin, sodium alginate oligosaccharide and sodium lauryl sulfate into solutions by weight respectively in water, and sequentially adding them into the mixture and stirring, thereby obtaining a fusion;

[0013] S4, raw material processing: Then, the nano-grade zinc oxide is ultrasonically dispersed in an ultrasonic disperser according to the weight portion to prepare a uniform nano-dispersion liquid.

[0014] S5, third mixing: then add the nano-dispersion liquid to the fusion through a dropper or a micro-injection pump, and add the modified polyether silicone by weight and stir, thereby completing the preparation.

[0015] Preferably, the S1 specifically comprises the following steps:

[0016] S101, putting thioflurane into a stirring mixer according to weight, and stirring at a rate of 300-400 rpm to preliminarily disperse it in the body;

[0017] S102, then add Jinggangmycin by weight, increase the stirring speed to 500-600 rpm, stir for 3-5 minutes, and make the two preliminarily mixed;

[0018] S103, then adding sodium lignin sulfonate and sodium carboxymethyl cellulose in parts by weight in sequence and stirring, increasing the stirring speed by 100-200 rpm after each addition, and extending the stirring time by 2-3 minutes;

[0019] S104, then add kaolin, and stir at a speed of 800-1000 rpm for 10-15 minutes to form a mixed powder.

[0020] Preferably, the pretreatment in S2 is to pour the Bacillus subtilis powder into a sterile container at a temperature of 25-30°C and filled with sterile water, stirring it at a stirring speed of 100-150 rpm and a stirring time of 3-5 minutes, and then placing it in a constant temperature incubator, controlling the activation temperature at 25-30°C, and culturing it for 2-3 hours. The addition rate of adding the powder to the mixed powder by a peristaltic pump or a dripping device for stirring is 10-20 ml per minute, the stirring speed is 300-500 rpm, and the stirring time is 5-8 minutes.

[0021] Preferably, the S3 specifically includes the following steps:

[0022] S301, stirring azadirachtin by weight with water at a temperature of 20-25° C. at a stirring speed of 200-300 rpm for 5-8 minutes;

[0023] S302, stirring the sodium alginate oligosaccharide by weight with water at a temperature of 30-40° C., preferably at a stirring speed of 150-250 rpm for 6-10 minutes;

[0024] S303, stirring sodium lauryl sulfate by weight with water at a temperature in the range of 20-25° C. at a stirring speed of 300-400 rpm for 3-5 minutes;

[0025] S304, then adding the stirred azadirachtin to the mixture, stirring at a speed of 400-600 rpm for 3-5 minutes, then adding the stirred sodium alginate oligosaccharide thereto, stirring at a speed of 500-700 rpm for 4-6 minutes, then adding the stirred sodium lauryl sulfate thereto, stirring at a speed of 600-800 rpm for 8-12 minutes, thereby obtaining a fusion.

[0026] Preferably, the ultrasonic dispersion in S4 is performed by placing nano-sized zinc oxide in an ultrasonic disperser by weight, and adding a dispersion medium 2-3 times its weight, performing ultrasonic dispersion at an ultrasonic frequency of 40-60 kHz for 15-20 minutes, pausing the ultrasound every 5 minutes, and shaking the container to evenly disperse the nanoparticles to obtain a nano-dispersion liquid.

[0027] Preferably, the rate of adding the nano-dispersion to the fusion by a dropper or a microinjection pump in S5 is 5-10 ml per minute, and the stirring speed of adding the modified polyether silicone by weight for stirring is 500-700 rpm, and the stirring time is 10-15 minutes.

[0028] The present invention provides a special control agent for rice sheath blight and a preparation method thereof, which has the following beneficial effects:

[0029] 1. The present invention integrates thiophanate-methyl and jinggangmycin, respectively, and starts from different mechanisms of action such as interfering with the energy metabolism of the pathogen and inhibiting the synthesis of the cell wall, to carry out precise strikes on the rice sheath blight pathogen, thereby improving the immediate sterilization effect. At the same time, biological enzymes such as chitinase and β-1,3-glucanase are used to target and decompose the key components of the bacterial cell wall. Even if the pathogen develops a certain resistance to chemical agents, the enzymes can still play a key role in breaking the defense, ensuring that the overall sterilization effect is long-lasting and stable, thereby solving the problem that the prevention and control effect of traditional rice sheath blight control agents is greatly reduced as the bacterial resistance gradually increases.

[0030] 2. The present invention introduces azadirachtin and Bacillus subtilis powder. Azadirachtin is derived from natural plants. Its inherent antibacterial properties will not bring additional burden to the surrounding ecological environment when inhibiting sheath blight pathogens, and there is no worry about residual toxic side effects. Bacillus subtilis powder, as a beneficial microbial preparation, can not only build a protective barrier on the surface of rice plants to resist the invasion of pathogens by secreting antibacterial substances, but also its own reproduction and metabolic process is integrated with the natural ecological cycle, reducing the use of chemical pesticides, thereby protecting ecological environmental elements such as soil and water bodies and maintaining the ecological balance in the field.

[0031] 3. The present invention deeply activates the defense signal pathway in rice through the introduction of sodium alginate oligosaccharides, warns of the attack of pathogens in advance, prompts the plant to start the self-protection mechanism, and stimulates the root system to grow vigorously, take root deeper and more steadily, and comprehensively improves the tolerance threshold to adverse environments such as drought and high temperature and sheath blight. At the same time, chitinase and β-1,3-glucanase release nutrients that can be absorbed by rice in the process of breaking down the cell walls of pathogens, nourishing the rice to grow vigorously, allowing the root system to develop and absorb nutrients more efficiently, and the stems to be thick and supportive with stronger strength, laying a solid foundation for high and excellent yields.

[0032] 4. The present invention utilizes nano-characteristics of nano-zinc oxide to achieve long-term antibacterial effect, and kaolin to function as a sustained-release carrier. Together, the two allow the effective ingredients of the agent to be released slowly and continuously. During the long rice growing season, a single application of the pesticide can provide long-term protection, reducing the tedious process and cost of repeated application of the pesticide, and reducing the risk of accumulated pesticide residues. At the same time, with the help of additives such as sodium lignin sulfonate, modified polyether silicone and sodium carboxymethyl cellulose, the agent will not be stratified, precipitated or agglomerated during storage, and will be evenly dispersed and perfectly attached during use. The efficacy is stable and consistent, providing a solid guarantee for field prevention and control operations. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The present invention provides a method flow chart for preparing a special control agent for rice sheath blight. DETAILED DESCRIPTION

[0034] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] The embodiment of the present invention provides a special control agent for rice sheath blight, comprising the following raw materials in parts by weight: 10-14 parts of thiofuran, 15-19 parts of validamycin, 8-12 parts of Bacillus subtilis powder, 3-5 parts of azadirachtin, 2-4 parts of chitinase, 1-3 parts of β-1,3-glucanase, 3-5 parts of sodium lignin sulfonate, 2-3 parts of modified polyether silicone, 1.5-2 parts of sodium carboxymethyl cellulose, 2-4 parts of sodium dodecyl sulfate, 3-5 parts of sodium alginate oligosaccharide, 1-2 parts of nano-grade zinc oxide, and 100 parts of kaolin; the effective viable count of the Bacillus subtilis powder is not less than 10 billion / g; the size range of the nano-grade zinc oxide is 1-100 nm; the activity of the chitinase is greater than 50 U / mg, and the activity of the β-1,3-glucanase is greater than 20 U / mg.

[0036] Specifically, by adding thiophanate-methyl, the succinate dehydrogenase activity of rice sheath blight pathogen is blocked, the energy metabolism pathway of the pathogen is cut off, and its growth and reproduction are restricted, thereby achieving strong inhibition of the pathogen. The spread of the pathogen can be quickly controlled in the early stage of infection, laying a solid foundation for the overall prevention and control effect of the agent.

[0037] By adding Jinggangmycin, the synthesis process of the bacterial cell wall is interfered with, causing the structural integrity of the bacterial cells to be damaged and eventually rupture and die, thereby achieving efficient sterilization, especially against the reproductive bodies of sheath blight fungi, thus ensuring the normal growth and development of rice.

[0038] By adding Bacillus subtilis powder, it colonizes on the surface and body of rice plants, secreting a variety of antibacterial substances such as iturin and surfactin, directly inhibiting the growth of sheath blight pathogens. At the same time, it induces the rice plants themselves to produce systemic resistance and enhances the ability of rice to resist pathogens, thereby achieving the dual effects of biological control and rice's own immune enhancement, and continuously protecting rice health in an eco-friendly way, thus solving the problem that single control with chemical agents is prone to cause pathogen resistance and environmental pollution.

[0039] By adding azadirachtin, it can interfere with the cellular physiological processes of pathogens with its broad-spectrum antibacterial properties, such as affecting the cell membrane permeability of pathogens, inhibiting the growth and metabolism of pathogens, thereby broadening the antibacterial spectrum of the agent, assisting in enhancing the bactericidal effect, and improving the agent's response capabilities in complex field pathogen environments.

[0040] By adding chitinase, the chitin component in the cell wall of the sheath blight fungus can be specifically decomposed, disintegrating the cell structure of the fungus from the inside, causing it to lose its protective barrier, thereby achieving precise attack on the vital parts of the fungus. Even if the fungus becomes resistant to some chemical agents, it can still play a key role, thus solving the problem of traditional agents becoming ineffective due to bacterial resistance and enhancing the overall combat effectiveness of the agents.

[0041] By adding β-1,3-glucanase, the β-1,3-glucan in the cell wall of the pathogen is hydrolyzed, and chitinase is used to further destroy the cell wall of the pathogen. At the same time, the rice's own immune response is stimulated, inducing the production of disease-resistant related proteins, thereby achieving synergistic enhancement with chitinase, destroying the pathogen's defense in multiple directions and enhancing the active defense ability of rice, thus solving the problem of the difficulty of long-term prevention and control relying solely on chemical sterilization and the susceptibility of rice itself.

[0042] By adding sodium lignin sulfonate, its dispersant function can be brought into play, so that the agent is evenly dispersed in the water, avoiding the agglomeration of agent particles, and ensuring that every rice plant can be evenly exposed to the effective ingredients during spraying, thereby achieving uniform application of the agent and ensuring consistent rice control effects in all parts of the field.

[0043] By adding modified polyether silicone, the surface tension of the pesticide solution is reduced, making it easier for the pesticide to spread on the surface of rice leaves and sheaths, and quickly penetrate into the interior of the plant tissues, so that the active ingredients can directly act on the sites of pathogen infection, thereby achieving rapid and accurate delivery of the pesticide to the sites of action and improving prevention and control efficiency.

[0044] By adding sodium carboxymethyl cellulose, it plays the role of a stabilizer, maintains the physical stability of the medicine, prevents stratification, precipitation and other phenomena, and ensures that the various properties of the medicine remain stable during storage and use, thereby ensuring that the quality of the medicine is reliable and available at any time.

[0045] By adding sodium dodecyl sulfate, on the one hand, the adhesion of the pesticide on the surface of the rice plant is improved, reducing the loss of the pesticide caused by factors such as rain erosion; on the other hand, the auxiliary dispersant further optimizes the dispersion effect of the pesticide and improves the utilization rate of the pesticide, thereby achieving long-term adhesion and efficient utilization of the pesticide and ensuring the overall prevention and control effect.

[0046] By adding sodium alginate oligosaccharides, the growth of rice roots is stimulated, making the rice plants stronger and enhancing their overall tolerance to sheath blight. At the same time, as a signal molecule, it activates the defense signal pathway in the rice body, starts the defense mechanism in advance, reduces the chance of bacterial infection, and helps alleviate disease symptoms after bacterial infection, thereby achieving enhanced prevention and control from two aspects: enhancing the intrinsic resistance of rice and active defense.

[0047] By adding nano-scale zinc oxide, its nano-size effect is utilized to produce highly oxidizing active oxygen substances, which directly kill the sheath blight bacteria. It can also attach more tightly to the surface of rice and even penetrate the plant cell wall into the intercellular space, continuously release active oxygen, and inhibit bacteria for a long time, thereby achieving efficient and lasting sterilization. It has a particularly outstanding striking effect on pathogens hidden inside rice tissues and those that are resistant to drugs, thereby further improving the problem that the prevention and control effects of traditional rice sheath blight control agents are greatly reduced as the drug resistance of the pathogens gradually increases.

[0048] Kaolin is used to make up to 100 parts, thereby providing the necessary filling volume for the medicine, facilitating molding and packaging. At the same time, its adsorptive property is used to adsorb active ingredients and auxiliary agents to ensure the stability of the medicine during storage. During use, as the carrier disperses, the active ingredients can be slowly released, playing a certain sustained-release effect, thereby achieving the convenience of storage and transportation of the medicine and its stability and long-term effect.

[0049] Please see attached Figure 1 A method for preparing a special control agent for rice sheath blight comprises the following steps:

[0050] S1, first mixing: adding thiofuran, validamycin, sodium lignin sulfonate, sodium carboxymethyl cellulose and kaolin into a stirring mixer in order by weight and stirring to obtain a mixed powder;

[0051] S2, adding bacterial powder: then pre-treating the Bacillus subtilis bacterial powder by weight, and then adding it into the mixed powder by a peristaltic pump or a dropping device, stirring it, so that it is evenly dispersed in the powder to obtain a mixture;

[0052] S3, second mixing: then dissolving azadirachtin, sodium alginate oligosaccharide and sodium lauryl sulfate into solutions by weight respectively in water, and sequentially adding them into the mixture and stirring, thereby obtaining a fusion;

[0053] S4, raw material processing: Then, the nano-grade zinc oxide is ultrasonically dispersed in an ultrasonic disperser according to the weight portion to prepare a uniform nano-dispersion liquid.

[0054] S5, third mixing: then add the nano-dispersion liquid into the fusion through a dropper or a micro-injection pump, and simultaneously add the modified polyether silicone according to weight and stir, thereby completing the preparation.

[0055] S1 specifically includes the following steps:

[0056] S101, putting thioflurane into a stirring mixer according to weight, and stirring at a rate of 300-400 rpm to preliminarily disperse it in the body;

[0057] S102, then add Jinggangmycin by weight, increase the stirring speed to 500-600 rpm, stir for 3-5 minutes, and make the two preliminarily mixed;

[0058] S103, then adding sodium lignin sulfonate and sodium carboxymethyl cellulose in parts by weight in sequence and stirring, increasing the stirring speed by 100-200 rpm after each addition, and extending the stirring time by 2-3 minutes;

[0059] S104, then add kaolin, and stir at a speed of 800-1000 rpm for 10-15 minutes to form a mixed powder.

[0060] The pretreatment in S2 is to pour the Bacillus subtilis powder into a sterile container with a temperature of 25-30°C and sterile water, stir it at a stirring speed of 100-150 rpm and a stirring time of 3-5 minutes, then place it in a constant temperature incubator, control the activation temperature at 25-30°C, culture it for 2-3 hours, add it to the mixed powder through a peristaltic pump or a dripping device, stir it at a rate of 10-20 ml per minute, stir it at a speed of 300-500 rpm, and stir it for 5-8 minutes.

[0061] S3 specifically includes the following steps:

[0062] S301, stirring azadirachtin by weight with water at a temperature of 20-25° C. at a stirring speed of 200-300 rpm for 5-8 minutes;

[0063] S302, stirring the sodium alginate oligosaccharide by weight with water at a temperature of 30-40° C., preferably at a stirring speed of 150-250 rpm for 6-10 minutes;

[0064] S303, stirring sodium lauryl sulfate by weight with water at a temperature in the range of 20-25° C. at a stirring speed of 300-400 rpm for 3-5 minutes;

[0065] S304, then adding the stirred azadirachtin to the mixture, stirring at a speed of 400-600 rpm for 3-5 minutes, then adding the stirred sodium alginate oligosaccharide thereto, stirring at a speed of 500-700 rpm for 4-6 minutes, then adding the stirred sodium lauryl sulfate thereto, stirring at a speed of 600-800 rpm for 8-12 minutes, thereby obtaining a fusion.

[0066] The ultrasonic dispersion in S4 is to put the nano-sized zinc oxide into an ultrasonic disperser according to the weight, and add a dispersion medium of 2-3 times of the weight thereof, and perform ultrasonic dispersion at an ultrasonic frequency of 40-60kHz for 15-20 minutes, and suspend the ultrasound every 5 minutes, and shake the container to evenly disperse the nanoparticles to obtain a nano-dispersion liquid.

[0067] In S5, the nano-dispersion liquid is added to the fusion by a dropper or a micro-injection pump at a rate of 5-10 ml per minute, and the modified polyether silicone is added by weight at a stirring speed of 500-700 rpm for 10-15 minutes.

[0068] The following is further introduced in conjunction with specific embodiments:

[0069] Embodiment 1:

[0070] A special control agent for rice sheath blight comprises the following raw materials in parts by weight: 14 parts of thiofuran, 19 parts of validamycin, 12 parts of bacillus subtilis powder, 5 parts of azadirachtin, 4 parts of chitinase, 3 parts of beta-1,3-glucanase, 5 parts of sodium lignin sulfonate, 3 parts of modified polyether silicone, 2 parts of sodium carboxymethyl cellulose, 4 parts of sodium dodecyl sulfate, 5 parts of sodium alginate oligosaccharide, 2 parts of nano-grade zinc oxide, and kaolin to make up to 100 parts.

[0071] The effective live bacteria count of Bacillus subtilis powder shall not be less than 10 billion / g.

[0072] The size of nano-ZnO is in the range of 10 nm.

[0073] The activity of chitinase is greater than 50U / mg, and the activity of β-1,3-glucanase is greater than 20U / mg.

[0074] A method for preparing a special-effect control agent for rice sheath blight, comprising the following steps:

[0075] S1, first mixing: adding thiofuran, validamycin, sodium lignin sulfonate, sodium carboxymethyl cellulose and kaolin into a stirring mixer in order by weight and stirring to obtain a mixed powder;

[0076] S2, adding bacterial powder: then pre-treating the Bacillus subtilis bacterial powder by weight, and then adding it into the mixed powder by a peristaltic pump or a dropping device, stirring it, so that it is evenly dispersed in the powder to obtain a mixture;

[0077] S3, second mixing: then dissolving azadirachtin, sodium alginate oligosaccharide and sodium lauryl sulfate into solutions by weight respectively in water, and sequentially adding them into the mixture and stirring, thereby obtaining a fusion;

[0078] S4, raw material processing: Then, the nano-grade zinc oxide is ultrasonically dispersed in an ultrasonic disperser according to the weight portion to prepare a uniform nano-dispersion liquid.

[0079] S5, third mixing: then add the nano-dispersion liquid into the fusion through a dropper or a micro-injection pump, and simultaneously add the modified polyether silicone according to weight and stir, thereby completing the preparation.

[0080] S1 specifically includes the following steps:

[0081] S101, adding thiothiocarb into a stirring mixer according to weight, stirring at a speed of 350 rpm to preliminarily disperse it in the body;

[0082] S102, then adding Jinggangmycin by weight, increasing the stirring speed to 550 rpm, stirring for 4 minutes to make the two preliminarily mixed;

[0083] S103, then adding sodium lignin sulfonate and sodium carboxymethyl cellulose in parts by weight in sequence and stirring, increasing the stirring speed by 150 rpm after each addition, and extending the stirring time by 2.5 minutes;

[0084] S104, then add kaolin, and stir at a speed of 900 rpm for 12.5 minutes to form a mixed powder.

[0085] The pretreatment in S2 is to pour the Bacillus subtilis powder into a sterile container at a temperature of 28°C and filled with sterile water, stir it at a stirring speed of 125 rpm and a stirring time of 4 minutes, then place it in a constant temperature incubator, control the activation temperature at 27°C, culture it for 2.5 hours, add it to the mixed powder through a peristaltic pump or a dripping device, and stir it at a rate of 15 ml per minute, a stirring speed of 400 rpm, and a stirring time of 6 minutes.

[0086] S3 specifically includes the following steps:

[0087] S301, stirring azadirachtin by weight with water at a temperature of 23° C. at a stirring speed of 250 rpm for 6 minutes;

[0088] S302, stirring the sodium alginate oligosaccharide by weight with water at a temperature of 35° C., preferably at a stirring speed of 200 rpm for 8 minutes;

[0089] S303, stirring sodium lauryl sulfate by weight with water at a temperature of 23° C. at a stirring speed of 330 rpm for 4 minutes;

[0090] S304, then adding the stirred azadirachtin to the mixture, stirring at a speed of 500 rpm for 4 minutes, then adding the stirred sodium alginate oligosaccharide thereto, stirring at a speed of 600 rpm for 5 minutes, then adding the stirred sodium lauryl sulfate thereto, stirring at a speed of 700 rpm for 10 minutes, thereby obtaining a fusion.

[0091] The ultrasonic dispersion in S4 is to put the nano-sized zinc oxide into an ultrasonic disperser according to the weight portion, and add a dispersion medium of 2.5 times of the weight thereof, and perform ultrasonic dispersion at an ultrasonic frequency of 50 kHz for 17 minutes, and suspend the ultrasound every 5 minutes, and shake the container to evenly disperse the nanoparticles, so as to obtain a nano-dispersion liquid.

[0092] In S5, the nano-dispersion liquid is added to the fusion by a dropper or a micro-injection pump at a rate of 7.5 ml per minute, and the modified polyether silicone is added by weight at a stirring speed of 600 rpm for 13 minutes.

[0093] Embodiment 2:

[0094] The difference between this embodiment and the above-mentioned embodiment 1 is that:

[0095] A special control agent for rice sheath blight comprises the following raw materials in parts by weight: 10 parts of thiofuran, 15 parts of validamycin, 8 parts of Bacillus subtilis powder, 3 parts of azadirachtin, 2 parts of chitinase, 1 part of β-1,3-glucanase, 3 parts of sodium lignin sulfonate, 2 parts of modified polyether silicone, 1.5 parts of sodium carboxymethyl cellulose, 2 parts of sodium dodecyl sulfate, 3 parts of sodium alginate oligosaccharide, 1 part of nano-grade zinc oxide, and kaolin to make up to 100 parts.

[0096] Embodiment 3:

[0097] The difference between this embodiment and the above-mentioned embodiment 1 is that:

[0098] A special control agent for rice sheath blight comprises the following raw materials in parts by weight: 12 parts of thiofuran, 17 parts of validamycin, 10 parts of bacillus subtilis powder, 4 parts of azadirachtin, 3 parts of chitinase, 2 parts of beta-1,3-glucanase, 4 parts of sodium lignin sulfonate, 2.5 parts of modified polyether silicone, 1.75 parts of sodium carboxymethyl cellulose, 3 parts of sodium dodecyl sulfate, 4 parts of sodium alginate oligosaccharide, 1.5 parts of nano-grade zinc oxide, and kaolin to make up to 100 parts.

[0099] Table 1:

[0100] contrast Example 1 Example 2 Example 3 Standard value Average inhibition zone diameter (mm) 32 28 30 15 Relative conductivity (%) 30 35 32 50 Residual amount of active ingredient on the seventh day (mg / kg) 80 60 70 20 Suspension rate (%) 90 85 88 80

[0101] The above table compares traditional rice sheath blight control agents. Table 1 shows that different amounts of thiophanate-methyl, jinggangmycin, chitinase, β-1,3-glucanase and nano-zinc oxide can affect the inhibition zone diameter of the agent, thereby synergizing from different angles such as inhibiting pathogen metabolism, destroying pathogen cell walls, and producing bactericidal active substances, thereby enhancing the bactericidal strength of the control agent. Different amounts of sodium alginate oligosaccharides and Bacillus subtilis powder affect the strength of the agent's function in enhancing rice stress resistance. This makes rice more resistant to sheath blight infection and other adverse environmental factors through the degree of the agent's effects on activating rice defense signal pathways, inducing rice to produce systemic resistance, and stimulating rice root growth, thereby reducing dependence on traditional bactericidal functions, thereby solving the problem that the control effect of traditional rice sheath blight control agents is greatly reduced as the resistance of pathogens gradually increases.

[0102] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A special control agent for rice sheath blight, characterized in that: The invention comprises the following raw materials in parts by weight: 10-14 parts of thiofuran, 15-19 parts of validamycin, 8-12 parts of bacillus subtilis powder, 3-5 parts of azadirachtin, 2-4 parts of chitinase, 1-3 parts of beta-1,3-glucanase, 3-5 parts of sodium lignin sulfonate, 2-3 parts of modified polyether silicone, 1.5-2 parts of sodium carboxymethyl cellulose, 2-4 parts of sodium dodecyl sulfate, 3-5 parts of sodium alginate oligosaccharide, 1-2 parts of nano-grade zinc oxide and kaolin to make up to 100 parts.

2. The rice sheath blight specific control agent according to claim 1, characterized in that: The effective live bacteria count of the Bacillus subtilis powder is not less than 10 billion / g.

3. The rice sheath blight specific control agent according to claim 1, characterized in that: The size of the nano-scale zinc oxide is in the range of 1-100 nm.

4. The rice sheath blight specific control agent according to claim 1, characterized in that: The activity of the chitinase is greater than 50 U / mg, and the activity of the β-1,3-glucanase is greater than 20 U / mg.

5. A method for preparing a special control agent for rice sheath blight, characterized in that: The special control agent for rice sheath blight as claimed in any one of claims 1 to 4 comprises the following steps: S1, first mixing: adding thiofuran, validamycin, sodium lignin sulfonate, sodium carboxymethyl cellulose and kaolin into a stirring mixer in order by weight and stirring to obtain a mixed powder; S2, adding bacterial powder: then pre-treating the Bacillus subtilis bacterial powder by weight, and then adding it into the mixed powder by a peristaltic pump or a dropping device, stirring it, so that it is evenly dispersed in the powder to obtain a mixture; S3, second mixing: then dissolving azadirachtin, sodium alginate oligosaccharide and sodium lauryl sulfate into solutions by weight respectively in water, and sequentially adding them into the mixture and stirring, thereby obtaining a fusion; S4, raw material processing: Then, the nano-grade zinc oxide is ultrasonically dispersed in an ultrasonic disperser according to the weight portion to prepare a uniform nano-dispersion liquid. S5, third mixing: then add the nano-dispersion liquid into the fusion through a dropper or a micro-injection pump, and simultaneously add the modified polyether silicone according to weight and stir, thereby completing the preparation.

6. The method for preparing a rice sheath blight specific control agent according to claim 5, characterized in that: The S1 specifically includes the following steps: S101, putting thioflurane into a stirring mixer according to weight, and stirring at a rate of 300-400 rpm to preliminarily disperse it in the body; S102, then add Jinggangmycin by weight, increase the stirring speed to 500-600 rpm, stir for 3-5 minutes, and make the two preliminarily mixed; S103, then adding sodium lignin sulfonate and sodium carboxymethyl cellulose in parts by weight in sequence and stirring, increasing the stirring speed by 100-200 rpm after each addition, and extending the stirring time by 2-3 minutes; S104, then add kaolin, and stir at a speed of 800-1000 rpm for 10-15 minutes to form a mixed powder.

7. The method for preparing a rice sheath blight specific control agent according to claim 5, characterized in that: The pretreatment in S2 is to pour the Bacillus subtilis powder into a sterile container with a temperature of 25-30°C and sterile water, stir it at a stirring speed of 100-150 rpm and a stirring time of 3-5 minutes, and then place it in a constant temperature incubator, control the activation temperature at 25-30°C, and culture it for 2-3 hours. The addition rate of adding the powder to the mixed powder through a peristaltic pump or a dripping device for stirring is 10-20 ml per minute, the stirring speed is 300-500 rpm, and the stirring time is 5-8 minutes.

8. The method for preparing a rice sheath blight specific control agent according to claim 5, characterized in that: The S3 specifically includes the following steps: S301, stirring azadirachtin by weight with water at a temperature of 20-25° C. at a stirring speed of 200-300 rpm for 5-8 minutes; S302, stirring the sodium alginate oligosaccharide by weight with water at a temperature of 30-40° C., preferably at a stirring speed of 150-250 rpm for 6-10 minutes; S303, stirring sodium lauryl sulfate by weight with water at a temperature in the range of 20-25° C. at a stirring speed of 300-400 rpm for 3-5 minutes; S304, then adding the stirred azadirachtin to the mixture, stirring at a speed of 400-600 rpm for 3-5 minutes, then adding the stirred sodium alginate oligosaccharide thereto, stirring at a speed of 500-700 rpm for 4-6 minutes, then adding the stirred sodium lauryl sulfate thereto, stirring at a speed of 600-800 rpm for 8-12 minutes, thereby obtaining a fusion.

9. The method for preparing a rice sheath blight specific control agent according to claim 5, characterized in that: The ultrasonic dispersion in S4 is to put the nano-sized zinc oxide into an ultrasonic disperser by weight, and add a dispersion medium of 2-3 times its weight, and perform ultrasonic dispersion at an ultrasonic frequency of 40-60kHz for 15-20 minutes, and suspend the ultrasound every 5 minutes, and shake the container to evenly disperse the nanoparticles to obtain a nano-dispersion liquid.

10. The method for preparing a rice sheath blight specific control agent according to claim 5, characterized in that: In S5, the nano-dispersion is added to the fusion by a dropper or a microinjection pump at a rate of 5-10 ml per minute, and the modified polyether silicone is added by weight at a stirring speed of 500-700 rpm for 10-15 minutes.

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