Application method of Streptomyces sp. Metabolite as growth regulator of Psychotria reeds

Abstract

The invention discloses an application of a Streptomyces sp. Liquid fermentation metabolite as a growth regulator of an edible fungus Ariobotrys reeds. The invention also discloses a preparation method of the Streptomyces sp. Liquid fermentation metabolite. The Streptomyces sp. Metabolite serving as the growth regulator of the edible fungus Acilia reeds has the following advantages that the process operation is simple, the metabolite can be used for preparation and cultivation of Acilia reeds strains only through solid-liquid separation or directly after liquid fermentation, the preparation period is short (the metabolite can be used after fermentation for 7 days), and industrialized production is easy. After the Streptomyces sp. Metabolite is added into the culture medium of the edible fungus Acilia reeds, the average growth rate of the Acilia reeds mycelium is higher than that of a control group, and the Acilia reeds mycelium is easier to culture.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus cultivation. Streptomyces sp. Application and method of liquid fermentation metabolites as growth regulators for the edible fungus Phragmites australis. Background Art

[0002] The cultivation process of edible fungi includes the preparation of fungus strains, the preparation of culture medium, inoculation and cultivation, and fruiting. Hygrophoropsis phragmiticola ), is a new species identified in recent years. Due to its special growth conditions, its traces are only found around Qilihai in Tianjin. It is characterized by extremely delicious taste, rich nutrition and excellent antioxidant capacity. In recent years, due to the gradually deteriorating ecological environment of Qilihai and artificial excessive collection, the natural reproduction and growth of wild reed wax umbrella has been seriously affected, resulting in a very small number of wild reed wax umbrellas. Artificial domestication and cultivation have become an important measure to save the wild resources of reed wax umbrella. Through searching, no patent disclosure documents related to the patent application of the present invention have been found. Summary of the invention

[0003] The present invention aims to provide a Streptomyces Streptomyces sp. Application and method of liquid fermentation metabolites as growth regulators for the edible fungus Phragmites australis.

[0004] The technical solution adopted by the present invention to solve the technical problem is: Streptomyces Streptomyces sp. Application of liquid fermentation metabolites as growth regulators for the edible fungus Phragmites australis.

[0005] Furthermore, the Streptomyces Streptomyces sp. The liquid fermentation metabolites were prepared by culturing in the following liquid culture medium, wherein the culture medium comprises the following components in parts by weight: see Table 1.

[0006] Table 1 Streptomyces sp. Liquid Metabolite Preparation Medium Reagent name Dosage / per 100mL Soluble starch 2g Potassium Nitrate 0.1g Magnesium sulfate 0.05g Sodium chloride 0.05g Dipotassium hydrogen phosphate 0.05g Ferrous Sulfate 0.001g Alternatively, the Streptomyces sp. The liquid fermentation metabolites can be used as a growth-promoting regulator for the hyphae of Pseudocerma pharagmidae and / or as a cultivation growth regulator for Pseudocerma pharagmidae; Alternatively, the Streptomyces sp. After liquid fermentation, solid-liquid separation is required (such as solid-liquid separation by centrifugation or plate-and-frame filtration) to obtain liquid Streptomyces sp. Metabolites; Or, use Streptomyces sp. The liquid fermentation metabolites are added into the culture medium of Phragmites australis and sterilized before use.

[0007] Further, 3-10 mL of the culture medium of 100 g of Phragmites australis was added. Streptomyces sp. Liquid fermentation metabolites.

[0008] A sort of Streptomyces sp. The method for applying liquid fermentation metabolites as a growth regulator for edible fungus Phragmites australis is characterized by comprising the following steps: (1) Culture preparation Streptomyces sp. Liquid fermentation metabolites; (2) The result obtained in step (1) Streptomyces sp. The liquid fermentation metabolites are added to the culture medium of Pseudocerma reedii, mixed and sterilized, and the culture medium can be used for the cultivation of the edible fungus Pseudocerma reedii. Among them, the Streptomyces sp. Liquid fermentation metabolites can be used as growth regulators for the edible fungus Phragmites australis.

[0009] Furthermore, in step (2) Streptomyces sp. The liquid fermentation metabolites can be used as a growth-promoting regulator for the hyphae of Pseudocerma phragmites and / or as a cultivation growth regulator for Pseudocerma phragmites.

[0010] Furthermore, when Streptomyces sp. When the liquid fermentation metabolite agent is used as a growth regulator for the mycelium of Pseudocercus reedii, 3-10 mL of the liquid fermentation metabolite agent is added to the culture medium of 100 g of Pseudocercus reedii mycelium. Streptomyces sp. Liquid fermentation metabolites; when Streptomyces sp When the liquid fermentation metabolite agent is used as a growth regulator for the cultivation of reed wax mushroom, 3-10 mL is added to every 100 g of reed wax mushroom culture medium. Streptomyces sp. Liquid fermentation metabolites; Alternatively, the culture temperature of the mycelium of Pseudomonas aeruginosa is 25° C.-28° C., and the culture time is 7-14 days; Alternatively, the cultivation time of the fruiting body of Phragmites australis is 50-60 days from the start of spawning.

[0011] Furthermore, the culture medium of the mycelium of Pseudomonas aeruginosa comprises the following components in percentage by weight: Wheat grain 2-10%, glucose 1-3%, peptone 0.1-0.3%, agar 1-2%, KH2PO4 0.1-0.3%, MgSO4·7H2O 0.1-0.2%, Streptomyces sp. Metabolite solution 3-10% (v / W 总 ); Alternatively, in step (1) Streptomyces sp. After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces sp. Metabolites.

[0012] use Streptomyces sp. A method for preparing reed parasol mushroom species from liquid fermentation metabolites, comprising the following steps: Streptomyces sp. The liquid fermentation metabolites are added to the culture medium of Pseudocyprinus reedii, and the plates are poured, and the Pseudocyprinus reedii blocks are inoculated, and the plates are cultured at 25° C.-28° C. for 7-14 days. The obtained strain is the Pseudocyprinus reedii strain, which can be used as the primary strain of the edible fungus Pseudocyprinus reedii; Among them, the Streptomyces sp. Liquid fermentation metabolites can be used as growth regulators for the edible fungus Phragmites australis.

[0013] The beneficial effects achieved by the present invention are: 1. The present invention Streptomyces sp. Metabolites have the following advantages as growth regulators for the edible fungus Phragmites australis: The process is simple to operate. After liquid fermentation, only solid-liquid separation is required or the liquid can be directly used for cultivation of Pseudocerma reedensis. The preparation cycle is short (currently fermentation generally takes 7 days) and is easy to produce in factories. Streptomyces sp. After the metabolites are produced, the mycelium of Pseudocercus reedii grows thick and dense, making it easier to cultivate. Streptomyces sp. Metabolites make the fungus grow faster and easier to produce mushrooms. Streptomyces sp. The metabolites can be used for the preparation of solid strains of the edible fungus Phragmites australis and the use of growth regulators for the cultivation of Phragmites australis.

[0014] The present invention Streptomyces sp. Liquid fermentation metabolites solve the technical problems of weak vitality of solid fungus strains of Pseudocerma reedensis and difficulty in domestication and cultivation. Pseudocerma reedensis is a new species. Its wild fungus has a short growth cycle in the Qilihai wetland and is more demanding on the nutritional growth environment. Currently, only one research unit has reported its cultivation method, and it has not been widely promoted. By adding Streptomyces sp. After the metabolites are produced, it becomes easy to produce mushrooms, which promotes the absorption of nutrients in the matrix by the reed wax cap, or in other words, promotes the vegetative reproductive growth of the reed wax cap. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 For the present invention Streptomyces sp. Slant culture results; Figure 2 For the present invention Streptomyces sp. Microscopic examination results; Figure 3 For the present invention Streptomyces sp. Liquid metabolites from shake flask cultures; Figure 4 The control group and the addition Streptomyces sp.Photos of mycelial growth of metabolites (measured after 12 days of cultivation); Figure 5 In the embodiments disclosed in the present invention, different concentrations of Streptomyces sp . Effect of metabolites on mycelial growth (measurement results after 12 days of cultivation) DETAILED DESCRIPTION

[0016] For a better understanding of the present invention, the present invention is further described in detail below in conjunction with embodiments, but the scope of protection claimed by the present invention is not limited to the scope represented by the embodiments.

[0017] The raw materials used in the present invention, unless otherwise specified, are all conventional commercially available products. The methods used in the present invention, unless otherwise specified, are all conventional methods in the art. The quality of each substance used in the present invention is the conventional quality.

[0018] Streptomyces sp. Application of liquid fermentation metabolites as growth regulators for the edible fungus Phragmites australis.

[0019] Preferably, the Streptomyces sp. The liquid fermentation metabolites were prepared by culturing in the following liquid culture medium, wherein the culture medium comprises the following components in parts by weight: see Table 2.

[0020] Table 2 Streptomyces sp. Liquid Metabolite Preparation Medium Reagent name Dosage / per 100mL Soluble starch 2g Potassium Nitrate 0.1g Magnesium sulfate 0.05g Sodium chloride 0.05g Dipotassium hydrogen phosphate 0.05g Ferrous Sulfate 0.001g Alternatively, the Streptomyces sp. The liquid fermentation metabolites can be used as a growth-promoting regulator for the mycelium of Pseudocerma pharagmidae and / or as a cultivation growth regulator for Pseudocerma pharagmidae; Alternatively, the Streptomyces sp. After liquid fermentation, solid-liquid separation is required (such as solid-liquid separation by centrifugation or plate-and-frame filtration) to obtain liquid Streptomyces sp. Metabolites; Or, use Streptomyces sp. The liquid fermentation metabolites are added into the culture medium of Phragmites australis and sterilized before use.

[0021] Preferably, 3-10 mL of Streptomyces sp. Liquid fermentation metabolites.

[0022] A sort of Streptomyces sp. The method for applying liquid fermentation metabolites as a growth regulator for edible fungus Phragmites australis is characterized by comprising the following steps: (1) Culture preparation Streptomyces sp. Liquid fermentation metabolites; (2) The result obtained in step (1) Streptomyces sp. The liquid fermentation metabolites are added to the culture medium of Pseudocerma reedii, mixed and sterilized, and the culture medium can be used for the cultivation of the edible fungus Pseudocerma reedii. Among them, the Streptomyces sp. Liquid fermentation metabolites can be used as growth regulators for the edible fungus Phragmites australis.

[0023] Preferably, in step (2) Streptomyces sp. The liquid fermentation metabolites can be used as a growth-promoting regulator for the mycelium of Pseudocerma phragmites and / or as a cultivation growth regulator for Pseudocerma phragmites.

[0024] Preferably, when Streptomyces sp. When the liquid fermentation metabolites are used as growth regulators for Pseudoparagineus pharagmidae mycelium, 3-10 mL of the liquid fermentation metabolites are added to the culture medium for every 100 g of Pseudoparagineus pharagmidae mycelium. Streptomyces sp. Liquid fermentation metabolites; when Streptomyces sp. When the liquid fermentation metabolites are used as growth regulators for the cultivation of Pseudocerma reed, 3-10 mL of liquid fermentation metabolites can be added to every 100 g of Pseudocerma reed culture medium. Streptomyces sp. Liquid fermentation metabolites; Alternatively, in step (1), Streptomyces sp. After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces sp. Metabolites.

[0025] use Streptomyces sp. A method for preparing reed parasol mushroom species from liquid fermentation metabolites, comprising the following steps: Streptomyces sp. The liquid fermentation metabolites are added to the culture medium of Pseudocyprinus reedii, and the plates are poured, and the Pseudocyprinus reedii blocks are inoculated, and the plates are cultured at 25° C.-28° C. for 7 to 14 days. The obtained strain is the Pseudocyprinus reedii strain, which can be used as the primary strain of the edible fungus Pseudocyprinus reedii. Among them, the Streptomyces sp. Liquid fermentation metabolites can be used as growth regulators for the edible fungus Phragmites australis.

[0026] Alternatively, the Streptomyces sp. After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces sp. Metabolites.

[0027] Specifically, the relevant preparation and application results are as follows: A sort of Streptomyces sp. The application and method of liquid fermentation metabolites as growth regulator of edible fungus Phragmites australis, and the preparation method thereof comprises the following steps: 1) Streptomyces sp. Cultivation and preparation of liquid fermentation metabolites; Said Streptomyces sp.The liquid culture medium used for liquid fermentation is shown in Table 1, and the results of liquid fermentation are shown in Figure 2 .from Figure 2 It can be seen that Streptomyces sp. Liquid culture works well.

[0028] 2) Performing solid-liquid separation on the liquid fermentation metabolite solution obtained in step 1) to obtain a metabolite solution.

[0029] 3) The obtained in step 2) Streptomyces sp. The metabolite solution is used as a growth regulator for mycelium culture and fruiting body cultivation of reed parasol mushroom.

[0030] The culture medium for the mycelium of Pseudomonas reedii includes the following components in parts by weight: 2-10% wheat grains, 1-3% glucose, 0.1-0.3% peptone, 1-2% agar, 0.1-0.3% KH2PO4, 0.1-0.2% MgSO4·7H2O, Streptomyces sp. Metabolite solution 3-10% (v / W 总 ).

[0031] The culture temperature of reed parasitoid mycelium is 25℃-28℃, and the culture time is 7-14 days. Figure 3 , Figure 4 ,pass Streptomyces sp. The results of the experiment on the intervention of different addition amounts of metabolites on the mycelium growth of Pseudomonas aeruginosa showed that after 10 days of culture in a constant temperature incubator, the mycelium growth radius of 3% and 5% addition amounts was significantly greater than that of the control group (no addition was made in the control group). Streptomyces sp. Liquid fermentation metabolites, only a common culture medium for the cultivation of Pseudomonas aeruginosa mycelium. Streptomyces sp. The metabolites significantly affected the growth of Pseudomonas aeruginosa mycelium.

[0032] The process of the present invention is simple to operate, has a short preparation cycle, and is easy to produce in factories. Streptomyces sp. Metabolites make the mycelium of Pseudocercus reedii grow thick and dense, making it easier to cultivate. Streptomyces sp. The metabolites make the fungus grow faster and mushrooms easier to produce. Streptomyces sp. The metabolites act as a growth regulator, promoting nutrient absorption and cell division during the growth process of Phragmites australis.

[0033] The method of the present invention can make the reed parasol mushroom species highly active and easy to produce mushrooms. Streptomyces sp. The metabolites have a significant promoting effect on the growth of Pseudocerma phacnogenolum and are easy to be industrialized and promoted in small workshops of farmers. Streptomyces sp.The metabolites act as a growth regulator, promoting nutrient absorption and cell division during the growth process of Phragmites australis.

[0034] It should be noted that in the embodiments of the present invention, v / w (ratio of volume to mass) refers to the ratio of mL to g or L to kg, that is, the dimensions are mL to g or L to kg.

[0035] Although the embodiments of the present invention are disclosed for illustrative purposes, those skilled in the art will appreciate that various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the contents disclosed in the embodiments.

Claims

1. Streptomyces Streptomyces sp. Application of liquid fermentation metabolites as growth regulators for the edible fungus Phragmites australis.

2. The use according to claim 1, characterized in that: Streptomyces Streptomyces sp. The liquid fermentation metabolites are prepared by culturing in the following liquid culture medium, wherein the culture medium comprises the following components in parts by weight: Alternatively, the Streptomyces Streptomyces sp. The liquid fermentation metabolite can be used as a growth regulator for the mycelium of Pseudocerma pharagmidae and / or as a cultivation growth regulator for Pseudocerma pharagmidae; Alternatively, the Streptomyces Streptomyces sp. After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces Streptomyces sp. Metabolites; Alternatively, Streptomyces Streptomyces sp. The liquid fermentation metabolites are added into the culture medium of Phragmites australis and sterilized before use.

3. The use according to claim 2, characterized in that: Add 3-10 mL of Streptomyces to the culture medium for every 100 g of Phragmites australis. Streptomyces sp. Liquid fermentation metabolites.

4. A Streptomyces Streptomyces sp. The application method of liquid fermentation metabolites as a growth regulator of edible fungus Phragmites australis is characterized by: The steps include: (1) Cultivation and preparation of Streptomyces Streptomyces sp. Liquid fermentation metabolites; (2) The Streptomyces obtained in step (1) Streptomyces sp. The liquid fermentation product is added to the culture medium of Pseudocerma reedii, mixed and sterilized, and the culture medium can be used for the cultivation of the edible fungus Pseudocerma reedii. Streptomyces sp. Liquid fermentation metabolites can be used as growth regulators for the edible fungus Phragmites australis.

5. The application method according to claim 4, characterized in that: In step (2), Streptomyces Streptomyces sp. The liquid fermentation metabolites can be used as a growth-promoting regulator for the hyphae of Pseudocerma phragmites and / or as a cultivation growth regulator for Pseudocerma phragmites.

6. The application method according to claim 5, characterized in that: When Streptomyces Streptomyces sp. When the liquid fermentation metabolite agent is used as a growth regulator for the mycelium of Pseudocercus reedii, 3-10 mL of Streptomyces is added to the culture medium of 100 g of Pseudocercus reedii. Streptomyces sp. Liquid fermentation metabolites; When Streptomyces Streptomyces sp. When liquid fermentation metabolites are used as growth regulators for the cultivation of Pseudocerma reed, 3-10 mL of Streptomyces is added to the culture medium of 100 g of Pseudocerma reed. Streptomyces sp. Liquid fermentation metabolites; Alternatively, the culture temperature of the mycelium of Pseudomonas aeruginosa is 25° C.-28° C., and the culture time is 7-14 days; Alternatively, the cultivation time of the fruiting body of Phragmites australis is 50-60 days from the start of spawning.

7. The use according to claim 6, characterized in that: The culture medium of the reed parasol mushroom mycelium comprises the following components in percentage by weight: Wheat grain 2-10%, glucose 0.1-0.3%, peptone 1-1.5%, KH2PO4 0.3-0.5%, MgSO4·7H2O 0.7-1%, Streptomyces Streptomyces sp. Liquid fermentation metabolite solution 3-10% (v / W 总 ); Alternatively, the cultivation medium for Phragmites australis includes the following components in percentage by weight: Here, v / w refers to the ratio of mL to g.

8. The application method according to any one of claims 4 to 7, characterized in that: In the step (1), Streptomyces Streptomyces sp. The liquid fermentation metabolites are prepared by culturing in the following liquid culture medium, wherein the culture medium comprises the following components in parts by weight: Alternatively, in step (1), Streptomyces Streptomyces sp. After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces Streptomyces sp. Metabolites.

9. A method for preparing Pseudomonas aeruginosa strains using liquid fermentation metabolites of Streptomyces sp., characterized in that: The steps are as follows: adding liquid fermentation metabolites of Streptomyces sp. to a culture medium of Pseudocyprinus pharagmidae, and pouring a plate; or after preparing a Pseudocyprinus pharagmidae plate, adding liquid fermentation metabolites of Streptomyces sp. to a culture medium of Pseudocyprinus pharagmidae, inoculating a Pseudocyprinus pharagmidae block, and culturing at 25°C-28°C for 7 to 14 days, and the obtained strain is a Pseudocyprinus pharagmidae strain, which can be used as a primary strain of the edible fungus Pseudocyprinus pharagmidae; The liquid fermentation metabolites of Streptomyces sp. can be used as a growth regulator for the edible fungus Phragmites australis.

10. The method according to claim 9, characterized in that: Streptomyces Streptomyces sp. The liquid fermentation metabolites are prepared by culturing in the following liquid culture medium, wherein the culture medium comprises the following components in parts by weight: Alternatively, the Streptomyces Streptomyces sp The liquid fermentation metabolites can be used as a growth-promoting regulator for the hyphae of Pseudocerma pharagmidae and / or as a cultivation growth regulator for Pseudocerma pharagmidae; Alternatively, the Streptomyces Streptomyces sp After liquid fermentation, solid-liquid separation is required to obtain liquid Streptomyces Streptomyces sp Metabolites.

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