Regulator for promoting seed germination and seedling growth

Abstract

The invention provides a conditioning agent for promoting seed germination and seedling growth, the conditioning agent comprises broccoli enzyme and a conditioning agent, and the conditioning agent is used for promoting seed germination and seedling growth under sodium chloride stress; the regulator disclosed by the invention can form a certain osmotic pressure environment around crops, and can relieve hyperosmotic pressure caused by sodium chloride to a certain extent; active oxygen can be removed, oxidative damage is reduced, and the integrity of seedling cells is protected; starch and protein stored in crops can be decomposed, and energy and material basis are provided for crop germination.

Description

Technical Field

[0001] The invention relates to the technical field of crop growth regulators, and in particular to a regulator for promoting seed germination and seedling growth. Background Art

[0002] In some broccoli planting bases, due to the huge scale of broccoli planting, part of the total amount of broccoli produced each year will be discarded during the planting, processing and sales process due to various reasons (discarded stems and leaves, underdeveloped bulbs, damage by pests and diseases, etc.), causing serious losses to farmers; in particular, a large number of discarded stems and leaves are produced each year, and except for a small part of these stems and leaves collected by farmers and directly used to feed herbivorous livestock, most of them are discarded in fields and ditches; the moisture content of these discarded stems and leaves is basically above 90%, which is very easy to rot. Directly discarding them will directly lead to environmental odor pollution and pollution of nearby water sources, which not only causes waste of available resources, but also leads to environmental problems such as air pollution and eutrophication of water bodies;

[0003] Studies have found that broccoli and other cauliflower plants generally contain glucosinolates, and the ITCs produced after their decomposition have a strong removal effect on soil microorganisms. Therefore, returning the discarded stems and leaves of broccoli to the field can be used as an economical means of removing soil-borne plant pathogens.

[0004] However, most of the above research is still theoretical. How to prepare regulators to promote crop growth based on broccoli is a problem that needs to be solved urgently. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a regulator for promoting seed germination and seedling growth, which solves the problems mentioned in the background technology.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] A regulator for promoting seed germination and seedling growth, comprising broccoli enzyme and a regulator, and the regulator is used for promoting seed germination and seedling growth.

[0008] Furthermore, the regulator includes polyglutamic acid, gibberellin, humic acid and calcium chloride.

[0009] Furthermore, the broccoli enzyme includes broccoli, sucrose, water, yeast, and a composite bacterial agent.

[0010] Furthermore, the composite bacterial agent includes Bacillus, EM bacteria, and Acetobacter.

[0011] Furthermore, the preparation method of the broccoli enzyme comprises the following steps:

[0012] Pretreatment of broccoli: Wash, drain and chop the broccoli, add the broccoli pieces, sucrose and water into a container in proportion and mix well;

[0013] First stage fermentation of broccoli: add 0.01% by mass of yeast, Bacillus and EM bacteria into the container, seal the container to start the first stage fermentation and stir the container at intervals;

[0014] The second stage fermentation of broccoli: The broccoli enzyme is produced by sealing the first stage fermentation.

[0015] Furthermore, in the first stage of fermentation, the interval stirring method is: the stirring frequency is 2 times a day, 4 minutes / time, and the first stage fermentation time is 5 days.

[0016] Furthermore, the fermentation temperature of the first stage and the second stage is 30.0±10°C.

[0017] Furthermore, the fermentation time of the second stage is 30 days.

[0018] The present invention provides a regulator for promoting seed germination and seedling growth. Compared with the prior art, the regulator has the following beneficial effects: the regulator of the present invention can form a certain osmotic pressure environment around crops, which can alleviate the high osmotic pressure caused by sodium chloride to a certain extent; it can remove active oxygen, reduce oxidative damage, and protect the integrity of seedling cells; it can decompose starch and protein stored inside crops, and provide energy and material basis for crop germination. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 The schematic diagram of the preparation process of broccoli enzyme of the present invention is shown. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In order to solve the technical problems in the background technology, a regulator for promoting seed germination and seedling growth is provided as follows:

[0023] The present invention provides a regulator for promoting seed germination and seedling growth, comprising broccoli enzyme and a regulator, and the regulator is used for promoting seed germination and seedling growth under sodium chloride stress.

[0024] In this embodiment, the regulator includes polyglutamic acid, gibberellin, humic acid and calcium chloride.

[0025] In the above scheme, polyglutamic acid is a biopolymer, which is polymerized from glutamic acid monomers. It has good water solubility and biocompatibility; in agriculture, it can improve the soil's ability to retain water and fertilizer. Because it can absorb and retain a large amount of water, just like a sponge, it reduces water loss, and at the same time, it can slowly release fertilizer in the soil to improve the utilization rate of fertilizer. Gibberellic acid is a plant hormone that plays an important regulatory role in the growth and development of plants; it can promote cell elongation, thereby making plant stems elongate and leaves larger. Humic acid is an organic substance formed by the decomposition and transformation of animal and plant residues by microorganisms and a series of chemical processes. It can also increase the cation exchange capacity in the soil and enhance the soil's ability to retain fertilizer. Calcium chloride is an inorganic salt with strong hygroscopicity and is easily soluble in water; in plants, calcium chloride can be used as a calcium source to supplement the calcium element required for plant growth. Calcium plays an important role in the formation and stability of plant cell walls and can enhance the stress resistance of plants, such as improving the resistance of plants to adverse environments such as drought and salt damage.

[0026] In this embodiment, the broccoli enzyme includes broccoli, sucrose, water, yeast, and a composite bacterial agent; the composite bacterial agent includes Bacillus, EM bacteria, and Acetobacter.

[0027] Broccoli is one of the main raw materials for enzyme production; it is rich in vitamins (such as vitamin C, vitamin K, vitamin B group, etc.), minerals (such as potassium, calcium, magnesium, etc.) as well as dietary fiber and various phytochemicals (such as sulforaphane). In enzyme production, these nutrients can provide a rich material basis for the growth and reproduction of microorganisms. At the same time, under the decomposition of microorganisms, the macromolecular nutrients in broccoli can be converted into small molecular substances that are easier for plants to absorb and utilize;

[0028] Sucrose is a high-quality carbon source. During the fermentation process, microorganisms (such as yeast, Bacillus, etc.) use sucrose for respiration to provide energy for their own metabolic activities.

[0029] Yeast plays a key role in the fermentation process. It can break down sugars such as sucrose into alcohol and carbon dioxide. The production of alcohol can inhibit the growth of harmful microorganisms to a certain extent and play a role in preservation.

[0030] Bacillus is a microorganism with multiple functions. It can secrete a variety of enzymes, such as amylase, protease, lipase, etc. These enzymes can break down the macromolecules in broccoli (such as starch, protein, fat, etc.) and convert them into small molecules of sugars, amino acids, fatty acids and other nutrients.

[0031] Bacillus can also produce some antibacterial substances to inhibit the growth of harmful microorganisms. Moreover, the spores formed during its growth process have strong resistance to stress and can survive in harsh environmental conditions, ensuring the stability of the fermentation process.

[0032] EM bacteria is a complex flora of various microorganisms, including lactic acid bacteria, yeast, photosynthetic bacteria, etc.

[0033] In this embodiment, the method for using the regulator includes: diluting the regulator by 200-800 times; and applying the diluted regulator to seeds or seedlings.

[0034] The above embodiments have the following effects:

[0035] Mechanisms by which Broccoli Enzyme Regulators promote seed and seedling growth:

[0036] Regulating osmotic pressure: Broccoli enzyme regulator contains some small molecular sugars and amino acids and other nutrients. These substances form a certain osmotic pressure environment around the seeds, which can alleviate the high osmotic pressure caused by sodium chloride to a certain extent.

[0037] Activate physiological activities inside seeds: Plant hormone analogs or microbial metabolites in enzyme regulators can activate the enzyme system inside seeds. These enzymes can decompose starch and protein stored inside seeds, providing energy and material basis for seed germination. At the same time, these substances can also regulate the hormone balance inside seeds, promote cell division and elongation, such as gibberellin analogs can promote the growth of radicle and plumule, so that seeds can germinate smoothly.

[0038] Antioxidant effect: A large amount of reactive oxygen species (ROS) are produced in plants, such as superoxide anions and hydrogen peroxide, which can cause oxidative damage to plant cells. Some antioxidant substances in broccoli enzyme regulators (such as vitamin C, polyphenols, etc.) can remove reactive oxygen species, reduce oxidative damage, and protect the integrity of seedling cells.

[0039] Regulating ion balance: Certain components of enzyme regulators may help regulate the ion balance in seedling cells. For example, it can promote the absorption of potassium ions (K+), which can exchange with sodium ions, reduce the accumulation of sodium ions in cells, and thus reduce the toxic effects of sodium ions.

[0040] In this embodiment, if Figure 1 As shown, the preparation method of the broccoli enzyme comprises the following steps:

[0041] Pretreatment of broccoli: Wash, drain and chop the broccoli, add the broccoli pieces, sucrose and water into a container in proportion and mix well;

[0042] First stage fermentation of broccoli: add 0.01% by mass of yeast, Bacillus and EM bacteria into the container, seal the container to start the first stage fermentation and stir the container at intervals;

[0043] The second stage fermentation of broccoli: The broccoli enzyme is produced by sealing the first stage fermentation.

[0044] In this embodiment, during the first stage of fermentation, the interval stirring method is: the stirring frequency is 2 times a day, 4 minutes / time, and the first stage of fermentation time is 5 days.

[0045] In this embodiment, the fermentation temperature of the first stage and the second stage is 30.0±10°C.

[0046] In this embodiment, the fermentation time of the second stage is 30 days.

[0047] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0048] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A regulator for promoting seed germination and seedling growth, characterized in that: The regulator comprises broccoli enzyme and a regulator, and the regulator is used for promoting seed germination and seedling growth under sodium chloride stress.

2. A regulator for promoting seed germination and seedling growth according to claim 1, characterized in that: The regulators include polyglutamic acid, gibberellin, humic acid and calcium chloride.

3. A regulator for promoting seed germination and seedling growth according to claim 1, characterized in that: The broccoli enzyme comprises broccoli, sucrose, water, yeast and a composite bacterial agent.

4. A regulator for promoting seed germination and seedling growth according to claim 3, characterized in that: The composite bacterial agent comprises bacillus, EM bacteria and acetobacter.

5. A regulator for promoting seed germination and seedling growth according to claim 1, characterized in that: The method of using the regulator includes: Dilute the regulator 200-800 times; Apply the diluted conditioner to seeds or seedlings.

6. A regulator for promoting seed germination and seedling growth according to claim 1, characterized in that: The preparation method of the broccoli enzyme comprises the following steps: Pretreatment of broccoli: Wash, drain and chop the broccoli, add the broccoli pieces, sucrose and water into a container in proportion and mix well; First stage fermentation of broccoli: add 0.01% by mass of yeast, Bacillus and EM bacteria into the container, seal the container to start the first stage fermentation and stir the container at intervals; The second stage fermentation of broccoli: The broccoli enzyme is produced by sealing the first stage fermentation.

7. A regulator for promoting seed germination and seedling growth according to claim 6, characterized in that: During the first stage of fermentation, the interval stirring method is: stirring frequency is 2 times a day, 4 minutes / time, and the first stage fermentation time is 5 days.

8. A regulator for promoting seed germination and seedling growth according to claim 6, characterized in that: The fermentation temperature of the first stage and the second stage is 30.0±10°C.

9. A regulator for promoting seed germination and seedling growth according to claim 6, characterized in that: The fermentation time of the second stage is 30 days.

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