Entomopathogen composition for preventing and treating tomato leaf miner as well as preparation method and application of entomopathogen composition

Abstract

The invention discloses an entomopathogenic composition for preventing and treating tomato leaf miners as well as a preparation method and application of the entomopathogenic composition, and belongs to the technical field of agriculture. The entomopathogenic composition for preventing and treating the tomato leaf miner comprises a symbiotic bacterium fermentation product compound agent of entomopathogenic nematodes sprayed on tomato leaves, a compound preparation of the entomopathogenic nematodes applied in holes when tomato seedlings are transplanted, and simulated leaf tomato moth trapping balls for trapping the tomato leaf miner. The invention further discloses a preparation method of the entomopathogenic composition for preventing and treating the tomato leaf miner and application of the entomopathogenic composition in prevention and treatment of the tomato leaf miner. The population density of imagoes in the greenhouse can be reduced; the chance that female tomato leaf miners lay eggs on leaves and fruits is reduced, the total egg laying amount in a shed is reduced, and the population density of larvae is reduced. Through the synergistic effect of a plurality of components, the effect of preventing and treating tomato leaf miners is achieved together.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and particularly relates to an insect pathogen composition for preventing and controlling tomato leafminer, and a preparation method and application thereof. Background Art

[0002] The tomato leafminer Tuta absoluta belongs to the Gelechiidae family of the Lepidoptera order. It is native to Peru in South America and is a very destructive leafminer pest. Since it was first discovered in Xinjiang, my country in 2017, it has rapidly spread to more than 20 provinces (autonomous regions, municipalities) in Southwest, Northwest, East China, North China, and Central China, with a clear trend of further expansion (Zhang et al., 2021). The tomato leafminer has a wide range of hosts, including more than 40 plant species from 9 families, including Solanaceae, Leguminosae, Cruciferae, and Poaceae. It is most fond of tomatoes and can cause damage to tomato plants throughout their growth period (Desneux et al., 2010). The tomato leafminer mainly feeds on the mesophyll by burrowing into the leaves as larvae, forming tiny tunnels; it can also bore into the fruit to form holes, causing premature aging of the plant and rot of the fruit. Generally, the tomato leafminer can reduce tomato production by 20% to 30%, and in severe cases, it can lead to a reduction of 80% to 100%. It is one of the most destructive pests in the tomato industry (Desneux et al., 2010; Zhang Guifen et al., 2018). my country ranks first in the world in tomato planting area, and is the third largest tomato grower and the largest exporter of processed tomato products (Food and Agriculture Organization, 2017). The invasion and colonization of the tomato leafminer poses a huge threat to the safe production of my country's tomato industry (Xian Xiaoqing et al., 2019).

[0003] The occurrence and damage of tomato leafminers are hidden. Adults usually lay eggs on the back or front of the top leaves or tender stems of the plants. After hatching, the larvae dive into the leaves or fruits to feed and cause damage. Most larvae develop to maturity and spin silk and droop, and pupate at a depth of 1 to 2 cm in the soil (Uchoa-Fernandes 1995; Biondi et al., 2018). Due to its hidden occurrence and hidden damage, chemical control is not completely effective and requires frequent use of drugs. et al.,1998;Guedesand 2012), long-term use will lead to increased resistance (Silva et al., 2016; Guedes et al., 2019; Oke et al., 2020; Li Xiaowei et al., 2022), and have adverse effects on pollinating insects and natural enemies, pollute the ecological environment, and cause risks to the planting industry, consumers and the environment (Zappalà et al., 2012; Zlof and Suffert, 2012).

[0004] At present, the prevention and control of tomato leafminers at different stages include: spraying chemical agents on the larval stage for prevention and control, such as chlorfenapyr and methoxyfenozide; using chemical pheromones to lure and kill male adults, reduce the number of male insects, and reduce egg laying. At present, chemical control is still the main measure for the prevention and control of tomato leafminers. However, chemical control is prone to cause problems such as rapid increase in pest resistance, environmental pollution, and poisoning of non-target objects. Therefore, there is an urgent need for a tomato leafminer control method with good control effect and environmental friendliness.

[0005] Insect pathogenic nematodes of the genera Steinernema and Heterorhabditis are a type of biological natural enemy resource with strong insecticidal ability, broad insecticidal spectrum and large-scale cultivation. This type of nematode has the ability to actively search and has a good control effect on hidden pests such as underground and borers (Georgis et al., 2006). It has been widely used in the control of pests in agriculture, forestry, pasture, flowers and sanitation (Ehlers, 2001; Gaugler et al., 2002; Labaude and Griffin, 2018), and also shows certain biological control potential for hidden tomato leafminers. Foreign researchers have conducted a large number of indoor studies on tomato leafminer larvae and screened out a variety of nematode species with high toxicity to larvae. How to effectively use insect pathogenic nematodes to control tomato leafminers in the field needs to be optimized. Summary of the invention

[0006] One of the purposes of the present invention is to provide an insect pathogen composition for controlling tomato leafminer, so as to solve the problem that tomato leafminer is highly harmful and difficult to control in the prior art.

[0007] The second object of the present invention is to provide a method for preparing the insect pathogenic composition.

[0008] The third object of the present invention is to provide an application of the insect pathogen composition.

[0009] A fourth object of the present invention is to provide a method for controlling tomato leafminer.

[0010] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0011] The first aspect of the present invention discloses an insect pathogenic composition for controlling tomato leafminers, comprising a compound preparation of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-carrying tomato moth-attracting ball for trapping female tomato leafminers to be laid;

[0012] Each 1L of the compound agent of the symbiotic bacteria fermentation product of insect pathogenic nematodes contains 100-400ml of the symbiotic bacteria fermentation liquid of insect pathogenic nematodes, 25-100g of the plant repellent of tomato leafminer, 2.5-10g of the laccase inhibitor, 0.5-2g of the moisturizer, 1.5-6g of the ethoxy-modified polytrisiloxane, and 1.5-6ml of the green peel orange oil, and the volume is made up with water;

[0013] Each 1L of the compound preparation of insect pathogenic nematodes contains 5,000,000-20,000,000 IJs of insect pathogenic nematodes infected and propagated by 5th-6th instar larvae of greater wax moth, 0.5-2 mol of polyphenol oxidase inhibitor, 2.5-10g of nematode moisturizer, and 50-300g of pest attractant, and the volume is made up with water;

[0014] The simulated leaf tomato moth-attracting ball comprises a simulated leaf tomato and a sponge rod soaked with 1-nonanol and / or ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0015] In some embodiments of the present invention, each 1L of the compound preparation of the symbiotic bacteria fermentation product of insect pathogenic nematodes contains 200ml of the symbiotic bacteria fermentation liquid of insect pathogenic nematodes, 50g of the tomato leafminer plant repellent, 5g of the laccase inhibitor, 1g of the moisturizer, 3g of the ethoxy-modified polytrisiloxane, and 3ml of the green peel orange oil, and the volume is made up with water;

[0016] Or / and each 1L of the compound preparation of insect pathogenic nematodes contains 10,000,000 IJs of insect pathogenic nematodes infected and propagated by 5th to 6th instar larvae of greater wax moth, 1 mol of polyphenol oxidase inhibitor, 5g of nematode moisturizer, and 65.5-262gg of insect attractant, and the volume is made up with water; preferably, the insect attractant is 131g;

[0017] Preferably, the insect attractants contained in each 1L of the compound preparation of insect pathogenic nematodes are as follows: 10-40g / L tomato paste, 15-60g / L casein, 10-40g / L wheat germ powder, 5-20g / L soybean powder, 2.5-10g / L sucrose, 4-16g / L glucose, 2.5-10g / L linoleic acid, 1.5-6g / L soybean oil, 5-20g / L vitamin C, and 10-40g / L cellulose;

[0018] Further preferably, the pest attractants contained in each 1L of the compound preparation of insect pathogenic nematodes are as follows: 20g / L tomato sauce, 30g / L casein, 20g / L wheat germ powder, 10g / L soybean powder, 5g / L sucrose, 8g / L glucose, 5g / L linoleic acid, 3g / L soybean oil, 10g / L vitamin C, and 20g / L cellulose.

[0019] In some embodiments of the present invention, the method for preparing the fermentation liquid of the symbiotic bacteria of insect pathogenic nematodes comprises the following steps: disinfecting and grinding the insect pathogenic nematodes in the infection period, aspirating a small amount of the nematode suspension onto an NBTA plate, culturing for 3 to 5 days at 28±0.5°C and in the dark, picking a blue-green single colony onto a new NBTA plate for further culturing, purifying repeatedly 2 to 3 times, picking a single colony onto an LB culture medium after purification, culturing at 28±0.5°C and 100 to 400 rpm for 8 to 24 hours to prepare a fermentation seed liquid, inoculating the seed liquid into a triangular flask containing an LB liquid culture medium at an inoculation rate of 1 to 4%, fermenting for 24 to 96 hours under the above conditions, and obtaining a supernatant by high-speed centrifugation, wherein the fermentation supernatant is the fermentation liquid of the symbiotic bacteria of insect pathogenic nematodes;

[0020] Preferably, the fermentation seed solution is prepared by culturing at 200 rpm for 15 hours.

[0021] Preferably, the seed solution is inoculated into a triangular flask containing LB liquid culture medium at a 2% inoculum amount;

[0022] Preferably, the fermentation is carried out for 48 hours, and the supernatant is obtained by high-speed centrifugation;

[0023] or / and the tomato leafminer plant repellent comprises rosemary powder;

[0024] or / and the laccase inhibitor comprises 2,3-dimercaptopropanol;

[0025] or / and the humectant comprises trehalose. In some embodiments of the present invention, the polyphenol oxidase inhibitor comprises lycopene; or / and the nematode humectant comprises polyacrylamide.

[0026] In some embodiments of the present invention, the preparation method of the insect pathogenic nematodes propagated by infection with the 5th to 6th instar larvae of the greater wax moth is as follows: the third instar larvae (IJs) of the insect pathogenic nematodes propagated by infection with the 5th to 6th instar larvae of the greater wax moth are collected into distilled water by the White trap method and stored in a refrigerator at 4°C for later use.

[0027] The second aspect of the present invention discloses a method for preparing an insect pathogenic composition for controlling tomato leafminers, which comprises preparing a compound preparation of symbiotic bacteria fermentation products, a compound preparation of insect pathogenic nematodes and a simulated leaf-carrying tomato moth-attracting ball respectively;

[0028] The preparation method of the compound agent of the fermentation product of the symbiotic bacteria of the insect pathogenic nematode comprises the following steps: taking the fermentation liquid of the symbiotic bacteria of the insect pathogenic nematode, the plant repellent of the tomato leafminer, the laccase inhibitor, the moisturizer, the ethoxy-modified polytrisiloxane and the green peel orange oil according to the formula, mixing them evenly, and making up the volume with water;

[0029] The compound preparation of insect pathogenic nematodes comprises the following steps: taking the components of insect pathogenic nematodes, polyphenol oxidase inhibitor, nematode moisturizer and pest attractant according to the formula, mixing them evenly, and making up the volume with water;

[0030] Alternatively, first mix the various components of the pest attractant evenly, then mix evenly with the formulated amount of insect pathogenic nematodes, polyphenol oxidase inhibitors, and nematode moisturizers, and make up the volume with water.

[0031] The preparation method of the simulated leaf tomato moth-attracting ball comprises the following steps: firstly, immersing a sponge stick into ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol, taking it out, drying it at room temperature until no liquid drips, and then inserting the sponge stick above the simulated leaf tomato.

[0032] In some embodiments of the present invention, when the sponge stick is immersed in a mixture of ethyl octanoate and 1-nonanol, the weight ratio of ethyl octanoate to 1-nonanol is 100:10-40, preferably 100:20.

[0033] The third aspect of the present invention discloses the use of an insect pathogenic composition for controlling tomato leafminer in controlling tomato leafminer.

[0034] The fourth aspect of the present invention discloses a method for controlling tomato leafminer, which is carried out using the above-mentioned insect pathogenic composition.

[0035] In some embodiments of the present invention, a compound of fermentation products of symbiotic bacteria of insect pathogenic nematodes is sprayed on tomato leaves at a rate of 15 to 20 L per mu of land;

[0036] When transplanting tomato seedlings, the compound preparation of insect pathogenic nematodes is evenly dripped on the roots of tomato seedlings. Preferably, the compound preparation of insect pathogenic nematodes is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center. More preferably, the dripping amount is 20 to 25 ml per seedling.

[0037] The simulated leaf-attached tomato moth-attracting balls are hung in a tomato field; preferably, the hanging height is 20 to 40 cm from the ground; preferably, 20 to 30 moth-attracting balls are hung per acre of land.

[0038] Laccase is an oxidase containing four copper ions and having o-diphenol oxidation activity. Laccase is involved in a variety of physiological functions of Lepidoptera insects. As a cell surface protein, laccase enables pathogens to evade immune recognition and has a multifunctional role in evading insect immune defense. In addition, laccase is essential for the construction and tanning of the cuticle of insects during pupation and eclosion. The symbiotic bacteria fermentation product compound of the insect pathogenic nematode of the present invention is provided with a laccase inhibitor to inhibit the laccase of the tomato leafminer larvae. After laccase is inhibited, the epidermis of the tomato leafminer larvae becomes lighter, the cuticle tans and softens, the body color becomes darker, and the growth and development are hindered while the immunity is suppressed.

[0039] The fermentation liquid of the symbiotic bacteria of insect pathogenic nematodes contains toxin proteins for the larvae of tomato leafminers, and the larvae of tomato leafminers feed on tomato leaves containing the fermentation products of the symbiotic bacteria and show a stomach poison effect. The green peel orange oil of the present invention is a pure natural plant essential oil, which is obtained from green peel oranges by adopting a low-temperature distillation extraction process, and its main component is D-limonene. The green peel orange oil can not only drive the fermentation liquid composite agent to be quickly absorbed by the plant leaf surface, but also improve the adhesion of the liquid on the plant leaf surface, effectively prevent rain erosion, improve the utilization rate of fertilizers and pesticides, and also has the effect of leaf insecticide and sterilization, is natural and residue-free, and has good safety.

[0040] Lycopene, molecular formula C 40 H 56 , is a low-toxic and high-activity polyphenol oxidase inhibitor extracted from tomatoes. With the help of the feed attractant effect, the mature larvae of the tomato leafminer actively feed on lycopene, which effectively inhibits the humoral immune function of the larvae, making it easier for insect pathogenic nematodes to infect and kill mature larvae and cocoons faster and easier, making it impossible for them to emerge as adults, and reducing the population density of adults in the greenhouse.

[0041] 1-Nonanol and ethyl octanoate have significant egg-laying attractant activity for tomato leafminers. Female tomato leafminers to be laid eggs will be attracted to simulated tomatoes (with leaves) to lay eggs, and will be stuck and die, reducing the chances of female tomato leafminers to lay eggs on leaves and fruits, and reducing the overall egg-laying amount in the shed (female insects cannot lay eggs if they die), and reducing the larval population density.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The present invention is scientifically designed and ingeniously conceived. It consists of three parts: a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes, a compound preparation of insect pathogenic nematodes, and a simulated leaf tomato moth-attracting ball. The compound of symbiotic bacteria fermentation products is sprayed on tomato leaves, and the compound preparation of insect pathogenic nematodes is evenly dripped on the roots of tomato seedlings when transplanting tomato seedlings, inhibiting the humoral immune function of tomato leafminer larvae, making it easier for insect pathogenic nematodes to infect deadly mature larvae and cocoons faster and easier, making it impossible for them to emerge into adults, and reducing the population density of adults in the shed; the simulated leaf tomato moth-attracting ball can reduce the chance of female tomato leafminers laying eggs on leaves and fruits, and reduce the overall egg-laying amount in the shed, reducing the population density of larvae. The present invention plays a role in preventing and controlling tomato leafminers through the synergistic effect of multiple components. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Attached Figure 1 A real picture of the simulated leaf-tomato moth-attracting ball of the present invention;

[0045] Attached Figure 2 This is the layout and survey arrangement of the experimental plot in the tomato greenhouse. DETAILED DESCRIPTION

[0046] The present invention is further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations. In the embodiments, if specific conditions are not specified, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0047] The glutinous rice glue described in the embodiment of the present invention is food grade glutinous rice glue.

[0048] The insect pathogenic nematode of the present invention is a commercial strain that is resistant to high temperatures. The insect pathogenic nematode used in the embodiments of the present invention is Steinernema carpocapsae insect pathogenic nematode.

[0049] The LB culture medium described in the present invention is a prior art. The LB culture medium in the embodiment of the present invention is composed of: 10 g / L tryptone, 5 g / L yeast extract, and 10 g / L sodium chloride.

[0050] Example 1

[0051] This embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition includes a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0052] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0053] Table 1 Formula of the compound preparation of the symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 1

[0054] composition content Fermentation broth of symbiotic bacteria of insect pathogenic nematodes 200ml / L Plant repellent for tomato leafminers: dried rosemary plants and powdered 50g / L Laccase inhibitor: 2,3-Bis-mercaptopropanol 5g / L Moisturizer-Trehalose 1g / L Ethoxy modified polytrisiloxane 3g / L Green orange oil 3ml / L water Make up the volume to 1L

[0055] The preparation method of the fermentation product compound of the original nematode is as follows:

[0056] S1. Preparation of symbiotic bacteria fermentation broth of insect pathogenic nematodes:

[0057] The newly obtained infective nematodes were soaked and disinfected in a formaldehyde solution with a volume fraction of 0.1% for 30 minutes each time, and washed with double distilled water during the period. Then, the nematodes were soaked in 1g / L sodium thiomercurate for another 30 minutes, washed with double distilled water 3 times after soaking, ground, and finally a small amount of nematode suspension was pipetted onto the NBTA plate and cultured at 28°C and dark conditions for 5 days. Pick a blue-green single colony to a new NBTA plate for further culture, and purify it repeatedly 3 times. After purification, pick a single colony to LB culture medium, culture at 28°C; 200rpm for 15 hours to make a fermentation seed liquid, inoculate the seed liquid into a triangular flask containing LB liquid culture medium with a 2% inoculation amount, ferment for 48 hours under the above conditions, and centrifuge at high speed to obtain the supernatant, which is the fermentation supernatant of insect pathogenic nematode symbiotic bacteria.

[0058] S2. Preparation of plant repellent: Rosemary plants are dried and powdered (80 mesh) to obtain rosemary powder as a plant repellent.

[0059] S3. Prepare the raw materials according to the formula, mix the raw materials except water evenly, and then add water to make up to the specified volume to obtain a compound agent of the fermentation product of the symbiotic bacteria of insect pathogenic nematodes.

[0060] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0061] Table 2 Formula of the compound preparation of insect pathogenic nematodes of Example 1

[0062]

[0063] The preparation method of the compound preparation of insect pathogenic nematodes is as follows:

[0064] Step (1): preparing insect pathogenic nematodes propagated by infection with 5-6 instar larvae of greater wax moth: the third instar larvae (IJs) of insect pathogenic nematodes propagated by infection with 5-6 instar larvae of greater wax moth are collected into distilled water by white trap method and stored in a refrigerator at 4°C for later use.

[0065] Step (2): Prepare the pest attractant: Mix the components of the pest attractant evenly according to the proportion and set aside:

[0066] Step (3): The insect pathogenic nematodes infected and propagated by the 5th to 6th instar larvae of greater wax moth, the pest attractant, lycopene and polyacrylamide are mixed evenly in proportion, and water is added to make up to a specified volume to obtain a gel-like compound preparation of insect pathogenic nematodes.

[0067] 3. Simulated leaf tomato moth-attracting ball

[0068] As attached Figure 1 As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0069] The preparation method of the sponge stick soaked with 1-nonanol and ethyl octanoate is as follows: immerse the sponge stick in ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol (weight ratio 100:20), take it out, and dry it at room temperature until no liquid drips.

[0070] Then insert the sponge stick soaked with 1-nonanol and ethyl caprylate into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0071] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0072] Example 2

[0073] This embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition includes a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0074] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0075] Table 3 Formula of the compound preparation of the symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 2

[0076] composition content Fermentation broth of symbiotic bacteria of insect pathogenic nematodes 300ml / L Plant repellent for tomato leafminers: dried rosemary plants and powdered 25g / L Laccase inhibitor: 2,3-Bis-mercaptopropanol 4g / L Moisturizer-Trehalose 0.5g / L Ethoxy modified polytrisiloxane 5g / L Green orange oil 4ml / L water Make up the volume to 1L

[0077] The preparation method of the fermentation product compound of the original nematode is as follows:

[0078] S1. Preparation of entomopathogenic nematode fermentation broth:

[0079] The newly obtained infective nematodes were soaked and disinfected in a 0.1% formaldehyde solution for 30 minutes each time, and washed with double distilled water during the period. Then, the nematodes were soaked in 1g / L sodium thiothiosalicylate for 30 minutes. After soaking, they were washed with double distilled water for 3 times, ground, and finally a small amount of nematode suspension was pipetted onto an NBTA plate and cultured at 28°C in the dark for 3 days. A blue-green single colony was picked up and continued to be cultured on a new NBTA plate, and purified repeatedly twice. After purification, a single colony was picked up and added to LB culture medium, and cultured at 28°C and 100rpm for 24 hours to prepare a fermentation seed liquid. The seed liquid was inoculated into a triangular flask containing LB liquid culture medium at a 4% inoculation amount, and fermented under the above conditions for 24 hours. The supernatant was obtained by high-speed centrifugation. The fermentation supernatant was the fermentation liquid of the symbiotic bacteria of insect pathogenic nematodes.

[0080] S2. Same as Example 1.

[0081] S3. Same as Example 1.

[0082] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0083] Table 4 Formula of the compound preparation of insect pathogenic nematodes of Example 2

[0084]

[0085] The preparation method of the compound preparation of insect pathogenic nematodes is as follows:

[0086] Step (1): preparing insect pathogenic nematodes propagated by infection with 5-6 instar larvae of greater wax moth: the third instar larvae (IJs) of insect pathogenic nematodes propagated by infection with 5-6 instar larvae of greater wax moth are collected into distilled water by white trap method and stored in a refrigerator at 4°C for later use.

[0087] Step (2): directly mix the insect pathogenic nematodes infected and propagated by the 5th to 6th instar larvae of greater wax moth, the components of the pest attractant, lycopene and polyacrylamide in proportion, add water to make up to a specified volume, and obtain a hydrogel-like compound preparation of insect pathogenic nematodes.

[0088] 3. Simulated leaf tomato moth-attracting ball

[0089] As attached Figure 1 As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0090] The preparation method of the sponge stick soaked with 1-nonanol and ethyl octanoate is as follows: immerse the sponge stick in ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol (weight ratio 100:10), take it out, and let it dry at room temperature until no liquid drips.

[0091] Then insert the sponge stick soaked with 1-nonanol and ethyl caprylate into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0092] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0093] Example 3

[0094] This embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition includes a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0095] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0096] Table 5 Formula of the compound preparation of the symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 3

[0097]

[0098]

[0099] The preparation method of the fermentation product compound of the original nematode is as follows:

[0100] S1. Preparation of entomopathogenic nematode fermentation broth:

[0101] The newly obtained infective nematodes were soaked and disinfected in a formaldehyde solution with a volume fraction of 0.1% for 30 minutes each time, and washed with double distilled water during the period. Then, the nematodes were soaked in 1g / L sodium thiothiosalicylate for another 30 minutes, washed with double distilled water 3 times after soaking, ground, and finally a small amount of nematode suspension was pipetted onto an NBTA plate and cultured at 28°C in the dark for 5 days. A blue-green single colony was picked up and continued to be cultured on a new NBTA plate, and purified repeatedly 3 times. After purification, a single colony was picked up and placed on an LB medium, cultured at 28°C and 400rpm for 8 hours to prepare a fermentation seed liquid, and the seed liquid was inoculated into a triangular flask containing LB liquid medium at a 1% inoculation amount, and fermented under the above conditions for 96 hours, and the supernatant was obtained by high-speed centrifugation. The fermentation supernatant was the fermentation liquid of insect pathogenic nematodes.

[0102] S2. Same as Example 1.

[0103] S3. Same as Example 1.

[0104] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0105] Table 6 Formula of the compound preparation of insect pathogenic nematodes of Example 3

[0106]

[0107]

[0108] The preparation method of the compound preparation of insect pathogenic nematodes is the same as that in Example 1.

[0109] 3. Simulated leaf tomato moth-attracting ball

[0110] As attached Figure 1 As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0111] The preparation method of the sponge stick soaked with 1-nonanol and ethyl octanoate is as follows: immerse the sponge stick in ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol (weight ratio 100:40), take it out, and dry it at room temperature until no liquid drips.

[0112] Then insert the sponge stick soaked with 1-nonanol and ethyl caprylate into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0113] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0114] Example 4

[0115] This embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition includes a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0116] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0117] Table 7 Formula of the compound agent of symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 4

[0118]

[0119]

[0120] The preparation method of the compound preparation of the fermentation product of the symbiotic bacteria of the original nematode is the same as that in Example 1.

[0121] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0122] Table 8 Formula of the compound preparation of insect pathogenic nematodes of Example 4

[0123]

[0124] The preparation method of the compound preparation of insect pathogenic nematodes is the same as that in Example 1.

[0125] 3. Simulated leaf tomato moth-attracting ball

[0126] As attached Figure 1 As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0127] The preparation method of the sponge stick soaked with 1-nonanol and ethyl octanoate is as follows: immerse the sponge stick in ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol (weight ratio 100:30), take it out, and dry it at room temperature until no liquid drips.

[0128] Then insert the sponge stick soaked with 1-nonanol and ethyl caprylate into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0129] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0130] Example 5

[0131] This embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition includes a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0132] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0133] Table 9 Formula of the compound agent of symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 5

[0134] composition content Fermentation broth of symbiotic bacteria of insect pathogenic nematodes 250ml / L Plant repellent for tomato leafminers: dried rosemary plants and powdered 40g / L Laccase inhibitor: 2,3-Bis-mercaptopropanol 3g / L Moisturizer-Trehalose 0.8g / L Ethoxy modified polytrisiloxane 4g / L Green orange oil 1.5ml / L water Make up the volume to 1L

[0135] The preparation method of the compound preparation of the fermentation product of the symbiotic bacteria of the original nematode is the same as that in Example 1.

[0136] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0137] Table 10 Formula of the compound preparation of insect pathogenic nematodes of Example 5

[0138]

[0139]

[0140] The preparation method of the compound preparation of insect pathogenic nematodes is the same as that in Example 1.

[0141] 3. Simulated leaf tomato moth-attracting ball

[0142] As attached Figure 1 As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0143] The preparation method of the sponge stick soaked with 1-nonanol is as follows: soak the sponge stick in ethyl octanoate, take it out, and let it dry at room temperature until no liquid drips.

[0144] Then insert the sponge stick soaked with 1-nonanol into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0145] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0146] Example 6

[0147] The embodiment discloses a preparation method of the insect pathogenic composition for controlling tomato leafminers of the present invention. The insect pathogenic composition comprises a compound of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound preparation of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-bearing tomato moth-attracting ball for trapping female tomato leafminers to be laid.

[0148] 1. The formula of the compound agent of the fermentation product of the symbiotic bacteria of the original nematode is shown in the following table:

[0149] Table 11 Formula of the compound agent of symbiotic bacteria fermentation product of insect pathogenic nematodes in Example 6

[0150] composition content Fermentation broth of symbiotic bacteria of insect pathogenic nematodes 150ml / L Plant repellent for tomato leafminers: dried rosemary plants and powdered 65g / L Laccase inhibitor: 2,3-Bis-mercaptopropanol 8g / L Moisturizer-Trehalose 2g / L Ethoxy modified polytrisiloxane 2.5 / L Green orange oil 5ml / L water Make up the volume to 1L

[0151] The preparation method of the compound preparation of the fermentation product of the symbiotic bacteria of the original nematode is the same as that in Example 1.

[0152] 2. The formula of the compound preparation of insect pathogenic nematodes is shown in the following table:

[0153] Table 12 Formula of the compound preparation of insect pathogenic nematodes of Example 6

[0154]

[0155]

[0156] The preparation method of the compound preparation of insect pathogenic nematodes is the same as that in Example 1.

[0157] 3. Simulated leaf tomato moth-attracting ball

[0158] As attached Figure 1As shown, the simulated leaf tomato moth-attracting ball includes a simulated leaf tomato and a sponge rod soaked with 1-nonanol and ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato and the leaf surface are coated with strong adhesive.

[0159] The preparation method of the sponge stick soaked with 1-nonanol and ethyl octanoate is as follows: immerse the sponge stick in ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol (weight ratio 100:40), take it out, and dry it at room temperature until no liquid drips.

[0160] Then insert the sponge stick soaked with 1-nonanol and ethyl caprylate into the top of the simulated leaf tomato, and then coat the surface of the simulated leaf tomato and the leaves with super glue.

[0161] When the insect pathogenic composition of this embodiment is used, the compound preparation of the symbiotic bacteria fermentation product of the insect pathogenic nematode is sprayed on the tomato leaves, and 15 to 20 L is sprayed per mu of land; when the tomato seedlings are transplanted, the compound preparation of the insect pathogenic nematode is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center, and the dripping amount is 20 to 25 ml per seedling; the simulated leaf tomato moth-attracting balls are hung in the tomato field; the hanging height is 20 to 40 cm from the ground; 20 to 30 are hung per mu of land.

[0162] Comparative Example 1

[0163] Compared with Example 1, this comparative example does not contain the fermentation product compound of insect pathogenic nematodes, and other conditions are the same.

[0164] Comparative Example 2

[0165] Compared with Example 1, the compound preparation of the fermentation product of the symbiotic bacteria of insect pathogenic nematodes in this comparative example does not contain the laccase inhibitor: 2,3-dimercaptopropanol, and the other conditions are the same.

[0166] Comparative Example 3

[0167] Compared with Example 1, the fermentation product compound of insect pathogenic nematodes in this comparative example does not contain rosemary plant dry powder, and other conditions are the same.

[0168] Comparative Example 4

[0169] Compared with Example 1, this comparative example does not contain the compound preparation of insect pathogenic nematodes, and other conditions are the same.

[0170] Comparative Example 5

[0171] Compared with Example 1, the simulated leaf tomato moth-attracting ball in this comparative example is not provided with a sponge rod soaked with 1-nonanol and ethyl octanoate, and the other conditions are the same.

[0172] Test Example 1

[0173] This test example examines the effects of the insect pathogenic composition of Example 1, Comparative Examples 1 to 5, and commercially available preparations on controlling tomato leafminers. The details are as follows:

[0174] 1. Trial Groups and Drugs

[0175] Table 13 Trial groups and drugs

[0176] Grouping drug Treatment 1 group The insect pathogenic composition of Example 1 Treatment 2 groups The insect pathogenic composition of comparative example 1 Treatment 3 groups The insect pathogenic composition of comparative example 2 Treatment 4 groups The insect pathogenic composition of comparative example 3 Treatment 5 groups The insect pathogenic composition of Comparative Example 4 Treatment 6 groups The insect pathogenic composition of comparative example 5 Blank control group No medication Conventional control group Chlorantraniliprole SC

[0177] 2. Test methods

[0178] 2.1 Test site

[0179] The field test site is located in the tomato planting base in Qingliu Town, Xindu District, Chengdu. The base is a greenhouse, facing north and south, 40m long from east to west and 12m wide. Three greenhouses that were severely damaged by tomato leafminers in previous years were selected for the test. The tomato seedlings in the greenhouses were transplanted in early March. Conventional pesticide treatment: 200g·L -1 Chlorantraniliprole suspension was used at a dosage of 20 ml / mu (FMC, USA), and was sprayed using a Delixi 16L electric backpack sprayer with a water consumption of 45L per mu; blank control group: no pesticide was applied.

[0180] 2.2 Test methods

[0181] The experiment was set up in 3 greenhouses, with 3 replicates. Each greenhouse was divided into 8 experimental plots, with 2m between each plot and 1m from the edge of the greenhouse. Each experimental plot was 8m long and 4m wide, and the treatment plots in the greenhouse were arranged in random blocks. The layout of the experimental plots and the survey layout in the tomato greenhouse are shown in the figure below. Figure 2 shown.

[0182] During the tomato harvest period in July, "W"-shaped evenly selected points were used in each plot. Five survey points were selected in each area, and five tomato plants were randomly surveyed at each point to record the number of plants and fruits damaged by tomato leafminers. Two leaves were randomly selected from the upper, middle and lower parts of each tomato plant, and the number of damaged leaves was recorded.

[0183] 2.3 Data Statistics and Analysis

[0184] The survey data were sorted by Excel 2019, and SPSS25.0 was used to perform one-way ANOVA (LSD test) to compare the difference levels among the treatments.

[0185] 3. Statistics and results

[0186] Table 14 Results of different preparations for controlling tomato leafminer

[0187] Number of damaged plants Number of damaged fruits Number of damaged leaves Treatment 1 group 5.0±1.0e 22.3±2.5e 33.3±3.2d Treatment 2 groups 9.7±1.5bc 32.7±2.1cd 43.3±4.0cd Treatment 3 groups 6.3±1.5de 43.7±2.1b 37.3±4.0cd Treatment 4 groups 8.0±2.0cd 36.7±4.7bcd 39.3±3.1cd Treatment 5 groups 11.0±1.0b 31.0±3.6d 46.3±4.7bc Treatment 6 groups 11.0±1.0b 41.7±3.1bc 54.7±6.5b Blank control group 13.7±1.5a 64.3±9.3a 77.3±9.7a Conventional control group 4.0±1.0e 33.7±6.7cd 23.0±5.6e

[0188] It can be seen from the above table:

[0189] (1) In terms of the number of damaged plants, treatment group 1 and the conventional control group performed best, with no significant difference between the two groups. They were significantly better than treatment group 2 (compound preparation of symbiotic bacteria fermentation products without insect pathogenic nematodes), treatment group 5 (compound preparation without insect pathogenic nematodes) and treatment group 6 (the simulated leaf tomato moth-attracting ball was not provided with a sponge stick soaked in 1-nonanol and ethyl octanoate). The blank control group had the highest number of damaged plants, which was significantly worse than all treatment groups. This indicates that the compound preparation of symbiotic bacteria fermentation products of insect pathogenic nematodes, the compound preparation of insect pathogenic nematodes and the sponge stick soaked in 1-nonanol and ethyl octanoate all played an extremely critical role in preventing and controlling the damage of tomato leafminers to tomato plants and were indispensable.

[0190] (2) In terms of the number of damaged fruits, treatment 1 had the best control effect and was significantly better than the conventional control control group and other treatment groups. The number of damaged fruits in treatment 6 (the simulated leaf tomato moth-attracting ball was not provided with a sponge bar soaked in 1-nonanol and ethyl octanoate) was significantly higher than that in treatment 1 and treatment 5, indicating that the conventional sponge bar soaked in 1-nonanol and ethyl octanoate played an extremely important role in protecting the fruit and was indispensable.

[0191] (3) In terms of the number of damaged leaves, the conventional control group performed best, followed by treatment 1. The blank control group had the highest number of damaged leaves, followed by treatment 6 and treatment 5. This indicates that the compound preparation of insect pathogenic nematodes and the sponge stick impregnated with 1-nonanol and ethyl octanoate play an extremely critical role in preventing and controlling the damage of tomato leafminers to tomato leaves and are indispensable.

[0192] 4. Conclusion

[0193] The group 1 treated with the insect pathogenic composition for controlling tomato leafminer of the present invention performed best in terms of the number of damaged plants and the number of damaged fruits, and had the best comprehensive control effect.

[0194] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An insect pathogenic composition for controlling tomato leafminer, characterized in that: It includes a compound agent of symbiotic bacteria fermentation products of insect pathogenic nematodes sprayed on tomato leaves, a compound agent of insect pathogenic nematodes applied in holes when tomato seedlings are transplanted, and a simulated leaf-carrying tomato moth-attracting ball for trapping female tomato leaf miners waiting to give birth; Each 1L of the compound agent of the symbiotic bacteria fermentation product of insect pathogenic nematodes contains 100-400ml of the symbiotic bacteria fermentation liquid of insect pathogenic nematodes, 25-100g of the plant repellent of tomato leafminer, 2.5-10g of the laccase inhibitor, 0.5-2g of the moisturizer, 1.5-6g of the ethoxy-modified polytrisiloxane, and 1.5-6ml of the green peel orange oil, and the volume is made up with water; Each 1L of the compound preparation of insect pathogenic nematodes contains 5,000,000-20,000,000 IJs of insect pathogenic nematodes infected and propagated by 5th-6th instar larvae of greater wax moth, 0.5-2 mol of polyphenol oxidase inhibitor, 2.5-10g of nematode moisturizer, and 50-300g of pest attractant, and the volume is made up with water; The simulated leaf tomato moth-attracting ball comprises a simulated leaf tomato and a sponge rod soaked with 1-nonanol and / or ethyl octanoate arranged thereon, and the surface of the simulated leaf tomato is coated with strong adhesive.

2. The insect pathogenic composition for controlling tomato leafminer according to claim 1, characterized in that: Each 1L of the compound agent of the symbiotic fermentation product of insect pathogenic nematodes contains 200ml of the fermentation liquid of the symbiotic bacteria of insect pathogenic nematodes, 50g of the plant repellent of tomato leafminer, 5g of the laccase inhibitor, 1g of the moisturizer, 3g of the ethoxy-modified polytrisiloxane, and 3ml of the green peel orange oil, and the volume is made up with water; Or / and each 1L of the compound preparation of insect pathogenic nematodes contains 10,000,000 IJs of insect pathogenic nematodes infected and propagated by 5th to 6th instar larvae of greater wax moth, 1 mol of polyphenol oxidase inhibitor, 5g of nematode moisturizer, and 65.5-262g of insect attractant, and the volume is made up with water; preferably, the insect attractant is 131g; Preferably, the insect attractants contained in each 1L of the compound preparation of insect pathogenic nematodes are as follows: 10-40g / L tomato paste, 15-60g / L casein, 10-40g / L wheat germ powder, 5-20g / L soybean powder, 2.5-10g / L sucrose, 4-16g / L glucose, 2.5-10g / L linoleic acid, 1.5-6g / L soybean oil, 5-20g / L vitamin C, and 10-40g / L cellulose; Further preferably, the pest attractants contained in each 1L of the compound preparation of insect pathogenic nematodes are as follows: 20g / L tomato sauce, 30g / L casein, 20g / L wheat germ powder, 10g / L soybean powder, 5g / L sucrose, 8g / L glucose, 5g / L linoleic acid, 3g / L soybean oil, 10g / L vitamin C, and 20g / L cellulose.

3. An insect pathogenic composition for controlling tomato leafminer according to claim 1 or 2, characterized in that: The preparation method of the symbiotic bacteria fermentation liquid of insect pathogenic nematodes comprises the following steps: disinfecting and grinding the insect pathogenic nematodes in the infection stage, absorbing a small amount of nematode suspension onto an NBTA plate, culturing for 3 to 5 days at 28±0.5°C and in the dark, picking a blue-green single colony onto a new NBTA plate for further culturing, purifying repeatedly for 2 to 3 times, picking a single colony onto an LB culture medium after purification, culturing at 28±0.5°C and 100 to 400 rpm for 8 to 24 hours to prepare a fermentation seed liquid, inoculating the seed liquid into a triangular flask containing an LB liquid culture medium at an inoculation amount of 1 to 4%, fermenting for 24 to 96 hours under the above conditions, and obtaining a supernatant by high-speed centrifugation, wherein the fermentation supernatant is the symbiotic bacteria fermentation liquid of insect pathogenic nematodes; or / and the tomato leafminer plant repellent comprises rosemary powder; or / and the laccase inhibitor comprises 2,3-dimercaptopropanol; Or / and the humectant includes trehalose.

4. The insect pathogenic composition for controlling tomato leafminer according to claim 1 or 2, characterized in that: The polyphenol oxidase inhibitor comprises lycopene; or / and the nematode moisturizing agent comprises polyacrylamide.

5. The insect pathogenic composition for controlling tomato leafminer according to claim 1 or 2, characterized in that: The preparation method of insect pathogenic nematodes propagated by infection with 5th to 6th instar larvae of greater wax moth is as follows: the third instar larvae (IJs) of insect pathogenic nematodes propagated by infection with 5th to 6th instar larvae of greater wax moth are collected into distilled water by White trap method and stored in a refrigerator at 4°C for later use.

6. The method for preparing the insect pathogenic composition for controlling tomato leafminer according to any one of claims 1 to 5, characterized in that: A compound preparation of symbiotic bacteria fermentation products, a compound preparation of insect pathogenic nematodes and a simulated leaf-carrying tomato moth-attracting ball are prepared respectively; The preparation method of the compound agent of the fermentation product of the symbiotic bacteria of the insect pathogenic nematode comprises the following steps: taking the fermentation liquid of the symbiotic bacteria of the insect pathogenic nematode, the plant repellent of the tomato leafminer, the laccase inhibitor, the moisturizer, the ethoxy-modified polytrisiloxane and the green peel orange oil according to the formula, mixing them evenly, and making up the volume with water; The compound preparation of insect pathogenic nematodes comprises the following steps: taking the components of insect pathogenic nematodes, polyphenol oxidase inhibitors, nematode moisturizers and pest attractants according to the formula, mixing them evenly, and making up the volume with water; or first mixing the components of the pest attractant evenly, and then mixing them evenly with the formulated amount of insect pathogenic nematodes, polyphenol oxidase inhibitors and nematode moisturizers, and making up the volume with water; The preparation method of the simulated leaf tomato moth-attracting ball comprises the following steps: firstly, immersing a sponge stick into ethyl octanoate or 1-nonanol or a mixture of ethyl octanoate and 1-nonanol, taking it out, drying it at room temperature until no liquid drips, and then inserting the sponge stick above the simulated leaf tomato.

7. The method for preparing an insect pathogenic composition for controlling tomato leafminer according to claim 6, characterized in that: When the sponge stick is immersed in the mixed solution of ethyl octanoate and 1-nonanol, the weight ratio of ethyl octanoate to 1-nonanol is 100:10-40, preferably 100:

20.

8. Use of the insect pathogenic composition for controlling tomato leafminer according to any one of claims 1 to 5 in controlling tomato leafminer.

9. A method for controlling tomato leafminer, characterized in that: The method is carried out using the insect pathogen composition described in any one of claims 1 to 5.

10. The method according to claim 9, characterized in that Spray the compound of the fermentation product of the symbiotic bacteria of insect pathogenic nematodes on tomato leaves, spraying 15-20L per mu of land; When transplanting tomato seedlings, the compound preparation of insect pathogenic nematodes is evenly dripped on the roots of tomato seedlings. Preferably, the compound preparation of insect pathogenic nematodes is evenly dripped in a circle with a diameter of 10 to 15 cm with the base of the seedling root as the center. More preferably, the dripping amount is 20 to 25 ml per seedling. The simulated leaf-attached tomato moth-attracting balls are hung in a tomato field; preferably, the hanging height is 20 to 40 cm from the ground; preferably, 20 to 30 moth-attracting balls are hung per acre of land.

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